Novel methods for the preparation and characterization of hyaluronan oligosaccharides of defined length.
Identifieur interne : 002403 ( PubMed/Checkpoint ); précédent : 002402; suivant : 002404Novel methods for the preparation and characterization of hyaluronan oligosaccharides of defined length.
Auteurs : D J Mahoney [Royaume-Uni] ; R T Aplin ; A. Calabro ; V C Hascall ; A J DaySource :
- Glycobiology [ 0959-6658 ] ; 2001.
Descripteurs français
- KwdFr :
- Acide hyaluronique (), Acide hyaluronique (isolement et purification), Animaux, Chondroïtines sulfate (), Chromatographie d'échange d'ions (), Chromatographie en phase liquide à haute performance (), Chromatographie sur gel (), Colorants fluorescents (), Cordon ombilical (), Humains, Hyaluronoglucosaminidase (métabolisme), Masse moléculaire, Mâle, Nouveau-né, Oligosaccharides (analyse), Oligosaccharides (isolement et purification), Ovis, Spectrométrie de masse MALDI (), Séquence glucidique, Électrophorèse ().
- MESH :
- analyse : Oligosaccharides.
- isolement et purification : Acide hyaluronique, Oligosaccharides.
- métabolisme : Hyaluronoglucosaminidase.
- Acide hyaluronique, Animaux, Chondroïtines sulfate, Chromatographie d'échange d'ions, Chromatographie en phase liquide à haute performance, Chromatographie sur gel, Colorants fluorescents, Cordon ombilical, Humains, Masse moléculaire, Mâle, Nouveau-né, Ovis, Spectrométrie de masse MALDI, Séquence glucidique, Électrophorèse.
English descriptors
- KwdEn :
- Animals, Carbohydrate Sequence, Chondroitin Sulfates (chemistry), Chromatography, Gel (methods), Chromatography, High Pressure Liquid (methods), Chromatography, Ion Exchange (methods), Electrophoresis (methods), Fluorescent Dyes (chemistry), Humans, Hyaluronic Acid (chemistry), Hyaluronic Acid (isolation & purification), Hyaluronoglucosaminidase (metabolism), Infant, Newborn, Male, Molecular Weight, Oligosaccharides (analysis), Oligosaccharides (isolation & purification), Sheep, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods), Umbilical Cord (chemistry).
- MESH :
- chemical , analysis : Oligosaccharides.
- chemical , chemistry : Chondroitin Sulfates, Fluorescent Dyes, Hyaluronic Acid.
- chemical , isolation & purification : Hyaluronic Acid, Oligosaccharides.
- chemical , metabolism : Hyaluronoglucosaminidase.
- chemistry : Umbilical Cord.
- methods : Chromatography, Gel, Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Electrophoresis, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
- Animals, Carbohydrate Sequence, Humans, Infant, Newborn, Male, Molecular Weight, Sheep.
Abstract
Hyaluronan is a ubiquitous glycosaminoglycan of high molecular weight that acts as a structural component of extracellular matrices and mediates cell adhesion. There have been numerous recent reports that fragments of hyaluronan have different properties compared to the intact molecule. Though many of these results may be genuine, it is possible that some activities are due to minor components in the preparations used. Therefore, it is important that well-characterized and highly purified oligosaccharides are used in cell biological and structural studies so that erroneous results are avoided. We present methods for the purification of hyaluronan oligomers of defined size using size exclusion and anion-exchange chromatography following digestion of hyaluronan with testicular hyaluronidase. These preparations were characterized by a combination of electrospray ionization mass spectrometry, matrix-assisted laser desorption/ionization mass spectrometry with time-of-flight analysis, and fluorophore-assisted carbohydrate electrophoresis. Hyaluronan oligomers ranging from tetrasaccharides to 34-mers were separated. The 4- to 16-mers were shown to be homogeneous with regard to length but did contain varying amounts of chondroitin sulfate. This contaminant could have been minimized if digestion had been performed with medical-grade hyaluronan rather than the relatively impure starting material used here. The 18- to 34-mer preparations were mixtures of oligosaccharides of different lengths (e.g., the latter contained 87% 34-mer, 10% 32-mer, and 3% 30-mer) but were free of detectable chondroitin sulfate. In addition to oligomers with even numbers of sugar rings, novel 5- and 7-mers with terminal glucuronic acid residues were identified.
DOI: 10.1093/glycob/11.12.1025
PubMed: 11805075
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:11805075Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Novel methods for the preparation and characterization of hyaluronan oligosaccharides of defined length.</title>
<author><name sortKey="Mahoney, D J" sort="Mahoney, D J" uniqKey="Mahoney D" first="D J" last="Mahoney">D J Mahoney</name>
<affiliation wicri:level="4"><nlm:affiliation>MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU</wicri:regionArea>
<orgName type="university">Université d'Oxford</orgName>
<placeName><settlement type="city">Oxford</settlement>
<region type="nation">Angleterre</region>
<region type="région" nuts="1">Oxfordshire</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Aplin, R T" sort="Aplin, R T" uniqKey="Aplin R" first="R T" last="Aplin">R T Aplin</name>
</author>
<author><name sortKey="Calabro, A" sort="Calabro, A" uniqKey="Calabro A" first="A" last="Calabro">A. Calabro</name>
</author>
<author><name sortKey="Hascall, V C" sort="Hascall, V C" uniqKey="Hascall V" first="V C" last="Hascall">V C Hascall</name>
</author>
<author><name sortKey="Day, A J" sort="Day, A J" uniqKey="Day A" first="A J" last="Day">A J Day</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2001">2001</date>
<idno type="RBID">pubmed:11805075</idno>
<idno type="pmid">11805075</idno>
<idno type="doi">10.1093/glycob/11.12.1025</idno>
<idno type="wicri:Area/PubMed/Corpus">002524</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002524</idno>
<idno type="wicri:Area/PubMed/Curation">002524</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002524</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002403</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002403</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Novel methods for the preparation and characterization of hyaluronan oligosaccharides of defined length.</title>
<author><name sortKey="Mahoney, D J" sort="Mahoney, D J" uniqKey="Mahoney D" first="D J" last="Mahoney">D J Mahoney</name>
<affiliation wicri:level="4"><nlm:affiliation>MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU</wicri:regionArea>
<orgName type="university">Université d'Oxford</orgName>
<placeName><settlement type="city">Oxford</settlement>
<region type="nation">Angleterre</region>
<region type="région" nuts="1">Oxfordshire</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Aplin, R T" sort="Aplin, R T" uniqKey="Aplin R" first="R T" last="Aplin">R T Aplin</name>
</author>
<author><name sortKey="Calabro, A" sort="Calabro, A" uniqKey="Calabro A" first="A" last="Calabro">A. Calabro</name>
</author>
<author><name sortKey="Hascall, V C" sort="Hascall, V C" uniqKey="Hascall V" first="V C" last="Hascall">V C Hascall</name>
</author>
<author><name sortKey="Day, A J" sort="Day, A J" uniqKey="Day A" first="A J" last="Day">A J Day</name>
</author>
</analytic>
<series><title level="j">Glycobiology</title>
<idno type="ISSN">0959-6658</idno>
<imprint><date when="2001" type="published">2001</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Carbohydrate Sequence</term>
<term>Chondroitin Sulfates (chemistry)</term>
<term>Chromatography, Gel (methods)</term>
<term>Chromatography, High Pressure Liquid (methods)</term>
<term>Chromatography, Ion Exchange (methods)</term>
<term>Electrophoresis (methods)</term>
<term>Fluorescent Dyes (chemistry)</term>
<term>Humans</term>
<term>Hyaluronic Acid (chemistry)</term>
<term>Hyaluronic Acid (isolation & purification)</term>
<term>Hyaluronoglucosaminidase (metabolism)</term>
<term>Infant, Newborn</term>
<term>Male</term>
<term>Molecular Weight</term>
<term>Oligosaccharides (analysis)</term>
<term>Oligosaccharides (isolation & purification)</term>
<term>Sheep</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)</term>
<term>Umbilical Cord (chemistry)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acide hyaluronique ()</term>
<term>Acide hyaluronique (isolement et purification)</term>
<term>Animaux</term>
<term>Chondroïtines sulfate ()</term>
<term>Chromatographie d'échange d'ions ()</term>
<term>Chromatographie en phase liquide à haute performance ()</term>
<term>Chromatographie sur gel ()</term>
<term>Colorants fluorescents ()</term>
<term>Cordon ombilical ()</term>
<term>Humains</term>
<term>Hyaluronoglucosaminidase (métabolisme)</term>
<term>Masse moléculaire</term>
<term>Mâle</term>
<term>Nouveau-né</term>
<term>Oligosaccharides (analyse)</term>
<term>Oligosaccharides (isolement et purification)</term>
<term>Ovis</term>
<term>Spectrométrie de masse MALDI ()</term>
<term>Séquence glucidique</term>
<term>Électrophorèse ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Oligosaccharides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Chondroitin Sulfates</term>
<term>Fluorescent Dyes</term>
<term>Hyaluronic Acid</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Hyaluronic Acid</term>
<term>Oligosaccharides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hyaluronoglucosaminidase</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Oligosaccharides</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Umbilical Cord</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Acide hyaluronique</term>
<term>Oligosaccharides</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Gel</term>
<term>Chromatography, High Pressure Liquid</term>
<term>Chromatography, Ion Exchange</term>
<term>Electrophoresis</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Hyaluronoglucosaminidase</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Carbohydrate Sequence</term>
<term>Humans</term>
<term>Infant, Newborn</term>
<term>Male</term>
<term>Molecular Weight</term>
<term>Sheep</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Acide hyaluronique</term>
<term>Animaux</term>
<term>Chondroïtines sulfate</term>
<term>Chromatographie d'échange d'ions</term>
<term>Chromatographie en phase liquide à haute performance</term>
<term>Chromatographie sur gel</term>
<term>Colorants fluorescents</term>
<term>Cordon ombilical</term>
<term>Humains</term>
<term>Masse moléculaire</term>
<term>Mâle</term>
<term>Nouveau-né</term>
<term>Ovis</term>
<term>Spectrométrie de masse MALDI</term>
<term>Séquence glucidique</term>
<term>Électrophorèse</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Hyaluronan is a ubiquitous glycosaminoglycan of high molecular weight that acts as a structural component of extracellular matrices and mediates cell adhesion. There have been numerous recent reports that fragments of hyaluronan have different properties compared to the intact molecule. Though many of these results may be genuine, it is possible that some activities are due to minor components in the preparations used. Therefore, it is important that well-characterized and highly purified oligosaccharides are used in cell biological and structural studies so that erroneous results are avoided. We present methods for the purification of hyaluronan oligomers of defined size using size exclusion and anion-exchange chromatography following digestion of hyaluronan with testicular hyaluronidase. These preparations were characterized by a combination of electrospray ionization mass spectrometry, matrix-assisted laser desorption/ionization mass spectrometry with time-of-flight analysis, and fluorophore-assisted carbohydrate electrophoresis. Hyaluronan oligomers ranging from tetrasaccharides to 34-mers were separated. The 4- to 16-mers were shown to be homogeneous with regard to length but did contain varying amounts of chondroitin sulfate. This contaminant could have been minimized if digestion had been performed with medical-grade hyaluronan rather than the relatively impure starting material used here. The 18- to 34-mer preparations were mixtures of oligosaccharides of different lengths (e.g., the latter contained 87% 34-mer, 10% 32-mer, and 3% 30-mer) but were free of detectable chondroitin sulfate. In addition to oligomers with even numbers of sugar rings, novel 5- and 7-mers with terminal glucuronic acid residues were identified.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11805075</PMID>
<DateCompleted><Year>2002</Year>
<Month>05</Month>
<Day>17</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>05</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0959-6658</ISSN>
<JournalIssue CitedMedium="Print"><Volume>11</Volume>
<Issue>12</Issue>
<PubDate><Year>2001</Year>
<Month>Dec</Month>
</PubDate>
</JournalIssue>
<Title>Glycobiology</Title>
<ISOAbbreviation>Glycobiology</ISOAbbreviation>
</Journal>
<ArticleTitle>Novel methods for the preparation and characterization of hyaluronan oligosaccharides of defined length.</ArticleTitle>
<Pagination><MedlinePgn>1025-33</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Hyaluronan is a ubiquitous glycosaminoglycan of high molecular weight that acts as a structural component of extracellular matrices and mediates cell adhesion. There have been numerous recent reports that fragments of hyaluronan have different properties compared to the intact molecule. Though many of these results may be genuine, it is possible that some activities are due to minor components in the preparations used. Therefore, it is important that well-characterized and highly purified oligosaccharides are used in cell biological and structural studies so that erroneous results are avoided. We present methods for the purification of hyaluronan oligomers of defined size using size exclusion and anion-exchange chromatography following digestion of hyaluronan with testicular hyaluronidase. These preparations were characterized by a combination of electrospray ionization mass spectrometry, matrix-assisted laser desorption/ionization mass spectrometry with time-of-flight analysis, and fluorophore-assisted carbohydrate electrophoresis. Hyaluronan oligomers ranging from tetrasaccharides to 34-mers were separated. The 4- to 16-mers were shown to be homogeneous with regard to length but did contain varying amounts of chondroitin sulfate. This contaminant could have been minimized if digestion had been performed with medical-grade hyaluronan rather than the relatively impure starting material used here. The 18- to 34-mer preparations were mixtures of oligosaccharides of different lengths (e.g., the latter contained 87% 34-mer, 10% 32-mer, and 3% 30-mer) but were free of detectable chondroitin sulfate. In addition to oligomers with even numbers of sugar rings, novel 5- and 7-mers with terminal glucuronic acid residues were identified.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mahoney</LastName>
<ForeName>D J</ForeName>
<Initials>DJ</Initials>
<AffiliationInfo><Affiliation>MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Aplin</LastName>
<ForeName>R T</ForeName>
<Initials>RT</Initials>
</Author>
<Author ValidYN="Y"><LastName>Calabro</LastName>
<ForeName>A</ForeName>
<Initials>A</Initials>
</Author>
<Author ValidYN="Y"><LastName>Hascall</LastName>
<ForeName>V C</ForeName>
<Initials>VC</Initials>
</Author>
<Author ValidYN="Y"><LastName>Day</LastName>
<ForeName>A J</ForeName>
<Initials>AJ</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Glycobiology</MedlineTA>
<NlmUniqueID>9104124</NlmUniqueID>
<ISSNLinking>0959-6658</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D005456">Fluorescent Dyes</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D009844">Oligosaccharides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9004-61-9</RegistryNumber>
<NameOfSubstance UI="D006820">Hyaluronic Acid</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9007-28-7</RegistryNumber>
<NameOfSubstance UI="D002809">Chondroitin Sulfates</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.2.1.35</RegistryNumber>
<NameOfSubstance UI="D006821">Hyaluronoglucosaminidase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002240" MajorTopicYN="N">Carbohydrate Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002809" MajorTopicYN="N">Chondroitin Sulfates</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002850" MajorTopicYN="N">Chromatography, Gel</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002852" MajorTopicYN="N">Chromatography, Ion Exchange</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004586" MajorTopicYN="N">Electrophoresis</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005456" MajorTopicYN="N">Fluorescent Dyes</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006820" MajorTopicYN="N">Hyaluronic Acid</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006821" MajorTopicYN="N">Hyaluronoglucosaminidase</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007231" MajorTopicYN="N">Infant, Newborn</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009844" MajorTopicYN="N">Oligosaccharides</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012756" MajorTopicYN="N">Sheep</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019032" MajorTopicYN="N">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014470" MajorTopicYN="N">Umbilical Cord</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year>
<Month>1</Month>
<Day>24</Day>
<Hour>10</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2002</Year>
<Month>5</Month>
<Day>23</Day>
<Hour>10</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2002</Year>
<Month>1</Month>
<Day>24</Day>
<Hour>10</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">11805075</ArticleId>
<ArticleId IdType="doi">10.1093/glycob/11.12.1025</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Royaume-Uni</li>
</country>
<region><li>Angleterre</li>
<li>Oxfordshire</li>
</region>
<settlement><li>Oxford</li>
</settlement>
<orgName><li>Université d'Oxford</li>
</orgName>
</list>
<tree><noCountry><name sortKey="Aplin, R T" sort="Aplin, R T" uniqKey="Aplin R" first="R T" last="Aplin">R T Aplin</name>
<name sortKey="Calabro, A" sort="Calabro, A" uniqKey="Calabro A" first="A" last="Calabro">A. Calabro</name>
<name sortKey="Day, A J" sort="Day, A J" uniqKey="Day A" first="A J" last="Day">A J Day</name>
<name sortKey="Hascall, V C" sort="Hascall, V C" uniqKey="Hascall V" first="V C" last="Hascall">V C Hascall</name>
</noCountry>
<country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Mahoney, D J" sort="Mahoney, D J" uniqKey="Mahoney D" first="D J" last="Mahoney">D J Mahoney</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002403 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 002403 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= PubMed |étape= Checkpoint |type= RBID |clé= pubmed:11805075 |texte= Novel methods for the preparation and characterization of hyaluronan oligosaccharides of defined length. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:11805075" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |