MersV1, PubMed, Checkpoint, bibRecord, 002317

Hyaluronan oligosaccharides induce CD44 cleavage and promote cell migration in CD44-expressing tumor cells.

Identifieur interne : 002317 ( PubMed/Checkpoint ); précédent : 002316; suivant : 002318

Hyaluronan oligosaccharides induce CD44 cleavage and promote cell migration in CD44-expressing tumor cells.

Auteurs : Kazuki N. Sugahara [Japon] ; Toshiyuki Murai ; Hitomi Nishinakamura ; Hiroto Kawashima ; Hideyuki Saya ; Masayuki Miyasaka

Source :

The Journal of biological chemistry [ 0021-9258 ] ; 2003.

RBID : pubmed:12801931

Descripteurs français

KwdFr :
- Acide hyaluronique (physiologie), Cellules cancéreuses en culture, Humains, Hydrolyse, Mouvement cellulaire, Oligosaccharides (physiologie).
MESH :
- physiologie : Acide hyaluronique, Oligosaccharides.
- Cellules cancéreuses en culture, Humains, Hydrolyse, Mouvement cellulaire.

English descriptors

KwdEn :
- Cell Movement, Humans, Hyaluronan Receptors (metabolism), Hyaluronic Acid (physiology), Hydrolysis, Oligosaccharides (physiology), Tumor Cells, Cultured.
MESH :
- chemical , metabolism : Hyaluronan Receptors.
- chemical , physiology : Hyaluronic Acid, Oligosaccharides.
- Cell Movement, Humans, Hydrolysis, Tumor Cells, Cultured.

Abstract

CD44 is an adhesion molecule that serves as a cell surface receptor for several extracellular matrix components, including hyaluronan (HA). The proteolytic cleavage of CD44 from the cell surface plays a critical role in the migration of tumor cells. Although this cleavage can be induced by certain stimuli such as phorbol ester and anti-CD44 antibodies in vitro, the physiological inducer of CD44 cleavage in vivo is unknown. Here, we demonstrate that HA oligosaccharides of a specific size range induce CD44 cleavage from tumor cells. Fragmented HA containing 6-mers to 14-mers enhanced CD44 cleavage dose-dependently by interacting with CD44, whereas a large polymer HA failed to enhance CD44 cleavage, although it bound to CD44. Examination using uniformly sized HA oligosaccharides revealed that HAs smaller than 36 kDa significantly enhanced CD44 cleavage. In particular, the 6.9-kDa HA (36-mers) not only enhanced CD44 cleavage but also promoted tumor cell motility, which was completely inhibited by an anti-CD44 monoclonal antibody. These results raise the possibility that small HA oligosaccharides, which are known to occur in various tumor tissues, promote tumor invasion by enhancing the tumor cell motility that may be driven by CD44 cleavage.

DOI: 10.1074/jbc.M300347200
PubMed: 12801931

Affiliations:

Japon

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pubmed:12801931

Le document en format XML

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<term>Oligosaccharides (physiology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Acide hyaluronique (physiologie)</term>
<term>Cellules cancéreuses en culture</term>
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<term>Hydrolyse</term>
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<front><div type="abstract" xml:lang="en">CD44 is an adhesion molecule that serves as a cell surface receptor for several extracellular matrix components, including hyaluronan (HA). The proteolytic cleavage of CD44 from the cell surface plays a critical role in the migration of tumor cells. Although this cleavage can be induced by certain stimuli such as phorbol ester and anti-CD44 antibodies in vitro, the physiological inducer of CD44 cleavage in vivo is unknown. Here, we demonstrate that HA oligosaccharides of a specific size range induce CD44 cleavage from tumor cells. Fragmented HA containing 6-mers to 14-mers enhanced CD44 cleavage dose-dependently by interacting with CD44, whereas a large polymer HA failed to enhance CD44 cleavage, although it bound to CD44. Examination using uniformly sized HA oligosaccharides revealed that HAs smaller than 36 kDa significantly enhanced CD44 cleavage. In particular, the 6.9-kDa HA (36-mers) not only enhanced CD44 cleavage but also promoted tumor cell motility, which was completely inhibited by an anti-CD44 monoclonal antibody. These results raise the possibility that small HA oligosaccharides, which are known to occur in various tumor tissues, promote tumor invasion by enhancing the tumor cell motility that may be driven by CD44 cleavage.</div>
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<Title>The Journal of biological chemistry</Title>
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<Abstract><AbstractText>CD44 is an adhesion molecule that serves as a cell surface receptor for several extracellular matrix components, including hyaluronan (HA). The proteolytic cleavage of CD44 from the cell surface plays a critical role in the migration of tumor cells. Although this cleavage can be induced by certain stimuli such as phorbol ester and anti-CD44 antibodies in vitro, the physiological inducer of CD44 cleavage in vivo is unknown. Here, we demonstrate that HA oligosaccharides of a specific size range induce CD44 cleavage from tumor cells. Fragmented HA containing 6-mers to 14-mers enhanced CD44 cleavage dose-dependently by interacting with CD44, whereas a large polymer HA failed to enhance CD44 cleavage, although it bound to CD44. Examination using uniformly sized HA oligosaccharides revealed that HAs smaller than 36 kDa significantly enhanced CD44 cleavage. In particular, the 6.9-kDa HA (36-mers) not only enhanced CD44 cleavage but also promoted tumor cell motility, which was completely inhibited by an anti-CD44 monoclonal antibody. These results raise the possibility that small HA oligosaccharides, which are known to occur in various tumor tissues, promote tumor invasion by enhancing the tumor cell motility that may be driven by CD44 cleavage.</AbstractText>
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Hyaluronan oligosaccharides induce CD44 cleavage and promote cell migration in CD44-expressing tumor cells.

Hyaluronan oligosaccharides induce CD44 cleavage and promote cell migration in CD44-expressing tumor cells.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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Pour générer des pages wiki