RAPD method useful for distinguishing Leishmania species: design of specific primers for L. braziliensis.
Identifieur interne : 002305 ( PubMed/Checkpoint ); précédent : 002304; suivant : 002306RAPD method useful for distinguishing Leishmania species: design of specific primers for L. braziliensis.
Auteurs : E. Martinez [Espagne] ; V. Alonso ; A. Quispe ; M C Thomas ; R. Alonso ; J E Pi Ero ; A C González ; A. Ortega ; B. ValladaresSource :
- Parasitology [ 0031-1820 ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Leishmaniose.
- génétique : ADN des protozoaires, Leishmania.
- ADN des protozoaires, Amorces ADN, Animaux, Clonage moléculaire, Humains, Hybridation d'acides nucléiques, Leishmania, Technique RAPD, Variation génétique.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA, Protozoan.
- chemical , genetics : DNA, Protozoan.
- chemical : DNA Primers.
- classification : Leishmania.
- diagnosis : Leishmaniasis.
- genetics : Leishmania.
- methods : Random Amplified Polymorphic DNA Technique.
- Animals, Cloning, Molecular, Genetic Variation, Humans, Nucleic Acid Hybridization.
Abstract
The technique of Random Amplification Polymorphic DNA allows fragments of the genome to be amplified by means of polymerase chain reaction (PCR) without previous knowledge of their sequences. The protozoa of the genus Leishmania present great genetic variability, making it difficult to characterize the different species. A method is developed with a single 10-mers long primer, which allows the species L. braziliensis, L. mexicana, L. infantum, L. tropica, L. chagasi, L. amazonensis and L. major to be differentiated. These products amplified by RAPD have also facilitated the design of some primers that amplify L. braziliensis DNA exclusively.
DOI: 10.1017/s0031182003004104
PubMed: 14700186
Affiliations:
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Quispe</name></author><author><name sortKey="Thomas, M C" sort="Thomas, M C" uniqKey="Thomas M" first="M C" last="Thomas">M C Thomas</name></author><author><name sortKey="Alonso, R" sort="Alonso, R" uniqKey="Alonso R" first="R" last="Alonso">R. Alonso</name></author><author><name sortKey="Pi Ero, J E" sort="Pi Ero, J E" uniqKey="Pi Ero J" first="J E" last="Pi Ero">J E Pi Ero</name></author><author><name sortKey="Gonzalez, A C" sort="Gonzalez, A C" uniqKey="Gonzalez A" first="A C" last="González">A C González</name></author><author><name sortKey="Ortega, A" sort="Ortega, A" uniqKey="Ortega A" first="A" last="Ortega">A. Ortega</name></author><author><name sortKey="Valladares, B" sort="Valladares, B" uniqKey="Valladares B" first="B" last="Valladares">B. 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The protozoa of the genus Leishmania present great genetic variability, making it difficult to characterize the different species. A method is developed with a single 10-mers long primer, which allows the species L. braziliensis, L. mexicana, L. infantum, L. tropica, L. chagasi, L. amazonensis and L. major to be differentiated. These products amplified by RAPD have also facilitated the design of some primers that amplify L. braziliensis DNA exclusively.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Martinez</LastName><ForeName>E</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Parasitology, Faculty of Pharmacy, University of La Laguna, Avda. 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Alonso</name><name sortKey="Alonso, V" sort="Alonso, V" uniqKey="Alonso V" first="V" last="Alonso">V. Alonso</name><name sortKey="Gonzalez, A C" sort="Gonzalez, A C" uniqKey="Gonzalez A" first="A C" last="González">A C González</name><name sortKey="Ortega, A" sort="Ortega, A" uniqKey="Ortega A" first="A" last="Ortega">A. Ortega</name><name sortKey="Pi Ero, J E" sort="Pi Ero, J E" uniqKey="Pi Ero J" first="J E" last="Pi Ero">J E Pi Ero</name><name sortKey="Quispe, A" sort="Quispe, A" uniqKey="Quispe A" first="A" last="Quispe">A. Quispe</name><name sortKey="Thomas, M C" sort="Thomas, M C" uniqKey="Thomas M" first="M C" last="Thomas">M C Thomas</name><name sortKey="Valladares, B" sort="Valladares, B" uniqKey="Valladares B" first="B" last="Valladares">B. Valladares</name></noCountry><country name="Espagne"><noRegion><name sortKey="Martinez, E" sort="Martinez, E" uniqKey="Martinez E" first="E" last="Martinez">E. 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