Elastogenic effects of exogenous hyaluronan oligosaccharides on vascular smooth muscle cells.
Identifieur interne : 002134 ( PubMed/Checkpoint ); précédent : 002133; suivant : 002135Elastogenic effects of exogenous hyaluronan oligosaccharides on vascular smooth muscle cells.
Auteurs : Binata Joddar [États-Unis] ; Anand RamamurthiSource :
- Biomaterials [ 0142-9612 ] ; 2006.
Descripteurs français
- KwdFr :
- Acide hyaluronique (), Acide hyaluronique (métabolisme), Acide hyaluronique (pharmacologie), Animaux, Aorte (cytologie), Cellules cultivées, Collagène (métabolisme), Collagène (ultrastructure), Fibrillines, Matrice extracellulaire (), Matrice extracellulaire (métabolisme), Muscles lisses vasculaires (cytologie), Myocytes du muscle lisse (), Myocytes du muscle lisse (cytologie), Myocytes du muscle lisse (métabolisme), Oligosaccharides (), Oligosaccharides (métabolisme), Oligosaccharides (pharmacologie), Prolifération cellulaire, Protéines des microfilaments (métabolisme), Rats, Régénération tissulaire guidée (), Élastine (métabolisme), Élastine (ultrastructure).
- MESH :
- cytologie : Aorte, Muscles lisses vasculaires, Myocytes du muscle lisse.
- métabolisme : Acide hyaluronique, Collagène, Matrice extracellulaire, Myocytes du muscle lisse, Oligosaccharides, Protéines des microfilaments, Élastine.
- pharmacologie : Acide hyaluronique, Oligosaccharides.
- Acide hyaluronique, Animaux, Cellules cultivées, Collagène, Fibrillines, Matrice extracellulaire, Myocytes du muscle lisse, Oligosaccharides, Prolifération cellulaire, Rats, Régénération tissulaire guidée, Élastine.
English descriptors
- KwdEn :
- Animals, Aorta (cytology), Cell Proliferation, Cells, Cultured, Collagen (metabolism), Collagen (ultrastructure), Elastin (metabolism), Elastin (ultrastructure), Extracellular Matrix (chemistry), Extracellular Matrix (metabolism), Fibrillins, Guided Tissue Regeneration (methods), Hyaluronic Acid (chemistry), Hyaluronic Acid (metabolism), Hyaluronic Acid (pharmacology), Microfilament Proteins (metabolism), Muscle, Smooth, Vascular (cytology), Myocytes, Smooth Muscle (cytology), Myocytes, Smooth Muscle (drug effects), Myocytes, Smooth Muscle (metabolism), Oligosaccharides (chemistry), Oligosaccharides (metabolism), Oligosaccharides (pharmacology), Rats.
- MESH :
- chemical , chemistry : Hyaluronic Acid, Oligosaccharides.
- chemical , metabolism : Collagen, Elastin, Hyaluronic Acid, Microfilament Proteins, Oligosaccharides.
- chemistry : Extracellular Matrix.
- cytology : Aorta, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle.
- drug effects : Myocytes, Smooth Muscle.
- metabolism : Extracellular Matrix, Myocytes, Smooth Muscle.
- methods : Guided Tissue Regeneration.
- chemical , pharmacology : Hyaluronic Acid, Oligosaccharides.
- chemical , ultrastructure : Collagen, Elastin.
- Animals, Cell Proliferation, Cells, Cultured, Fibrillins, Rats.
Abstract
Prior studies suggest that hyaluronan (HA), a glycosaminoglycan, may upregulate innately poor elastin matrix synthesis by adult vascular smooth muscle cells (SMCs). HA scaffolds could thus be useful to regenerate damaged vascular elastin. In an earlier study, we established that the elastogenic effects of non-oligomeric HA are fragment size- and/or dose-specific. We currently investigate the pro-elastogenic effects of exogenous HA oligomers on rat aortic smooth muscle cells (RASMCs). RASMCs were cultured with pure HA oligomers (4-mers) and mixtures (4-8mers) obtained by enzymatic digestion of long-chain HA (MW approximately 2000kDa). Polyacrylamide gel electrophoresis (PAGE)/Matrix Assisted Laser Desorption/Ionization Spectroscopy Time-Of-Flight Analysis (MALDI-TOF) showed HA digestates to contain a mixture of 4-8mers with a predominance of 4-mers (75+/-0.4% w/w). Cell layers supplemented with both pure HA 4-mers or oligomer mixtures showed proliferation levels similar to non-HA controls over 21 days of culture. Pure 4-mers and oligomer mixtures enhanced DNA-normalized output of tropoelastin by 1.6 and 1.8 times, respectively, and that of matrix elastin by approximately 2.7 times relative to controls. Sodium dodecyl sulfate (SDS)-PAGE/Western Blot and a desmosine assay semi-quantitatively confirmed the observed biochemical trends for tropoelastin and matrix elastin, respectively. HA oligomers induced enhanced synthesis of the elastin crosslinker, desmosine, and appeared to stabilize the elastin matrix by suppression of elastin-laminin receptor (ELR) activity relative to controls. Transmission electron micrographs (TEMs) showed elastin deposits within oligomer-supplemented cultures to be distinct, longitudinally oriented, aggregating fibrils, and clumps, and to be less abundant and mostly amorphous in controls. HA oligomers preserved normal fibrillin-mediated elastin matrix deposition. Results suggest that HA oligos are highly pro-elastogenic, promote elastin fibril formation, and stabilize elastin matrix and may thus be usefully incorporated into scaffolds for guided elastin regeneration.
DOI: 10.1016/j.biomaterials.2006.07.020
PubMed: 16899292
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:16899292Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Elastogenic effects of exogenous hyaluronan oligosaccharides on vascular smooth muscle cells.</title>
<author><name sortKey="Joddar, Binata" sort="Joddar, Binata" uniqKey="Joddar B" first="Binata" last="Joddar">Binata Joddar</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Bioengineering, Clemson University, Charleston, SC 29425, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Bioengineering, Clemson University, Charleston, SC 29425</wicri:regionArea>
<placeName><region type="state">Caroline du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ramamurthi, Anand" sort="Ramamurthi, Anand" uniqKey="Ramamurthi A" first="Anand" last="Ramamurthi">Anand Ramamurthi</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2006">2006</date>
<idno type="RBID">pubmed:16899292</idno>
<idno type="pmid">16899292</idno>
<idno type="doi">10.1016/j.biomaterials.2006.07.020</idno>
<idno type="wicri:Area/PubMed/Corpus">002232</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002232</idno>
<idno type="wicri:Area/PubMed/Curation">002232</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002232</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002134</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002134</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Elastogenic effects of exogenous hyaluronan oligosaccharides on vascular smooth muscle cells.</title>
<author><name sortKey="Joddar, Binata" sort="Joddar, Binata" uniqKey="Joddar B" first="Binata" last="Joddar">Binata Joddar</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Bioengineering, Clemson University, Charleston, SC 29425, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Bioengineering, Clemson University, Charleston, SC 29425</wicri:regionArea>
<placeName><region type="state">Caroline du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ramamurthi, Anand" sort="Ramamurthi, Anand" uniqKey="Ramamurthi A" first="Anand" last="Ramamurthi">Anand Ramamurthi</name>
</author>
</analytic>
<series><title level="j">Biomaterials</title>
<idno type="ISSN">0142-9612</idno>
<imprint><date when="2006" type="published">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Aorta (cytology)</term>
<term>Cell Proliferation</term>
<term>Cells, Cultured</term>
<term>Collagen (metabolism)</term>
<term>Collagen (ultrastructure)</term>
<term>Elastin (metabolism)</term>
<term>Elastin (ultrastructure)</term>
<term>Extracellular Matrix (chemistry)</term>
<term>Extracellular Matrix (metabolism)</term>
<term>Fibrillins</term>
<term>Guided Tissue Regeneration (methods)</term>
<term>Hyaluronic Acid (chemistry)</term>
<term>Hyaluronic Acid (metabolism)</term>
<term>Hyaluronic Acid (pharmacology)</term>
<term>Microfilament Proteins (metabolism)</term>
<term>Muscle, Smooth, Vascular (cytology)</term>
<term>Myocytes, Smooth Muscle (cytology)</term>
<term>Myocytes, Smooth Muscle (drug effects)</term>
<term>Myocytes, Smooth Muscle (metabolism)</term>
<term>Oligosaccharides (chemistry)</term>
<term>Oligosaccharides (metabolism)</term>
<term>Oligosaccharides (pharmacology)</term>
<term>Rats</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acide hyaluronique ()</term>
<term>Acide hyaluronique (métabolisme)</term>
<term>Acide hyaluronique (pharmacologie)</term>
<term>Animaux</term>
<term>Aorte (cytologie)</term>
<term>Cellules cultivées</term>
<term>Collagène (métabolisme)</term>
<term>Collagène (ultrastructure)</term>
<term>Fibrillines</term>
<term>Matrice extracellulaire ()</term>
<term>Matrice extracellulaire (métabolisme)</term>
<term>Muscles lisses vasculaires (cytologie)</term>
<term>Myocytes du muscle lisse ()</term>
<term>Myocytes du muscle lisse (cytologie)</term>
<term>Myocytes du muscle lisse (métabolisme)</term>
<term>Oligosaccharides ()</term>
<term>Oligosaccharides (métabolisme)</term>
<term>Oligosaccharides (pharmacologie)</term>
<term>Prolifération cellulaire</term>
<term>Protéines des microfilaments (métabolisme)</term>
<term>Rats</term>
<term>Régénération tissulaire guidée ()</term>
<term>Élastine (métabolisme)</term>
<term>Élastine (ultrastructure)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Hyaluronic Acid</term>
<term>Oligosaccharides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Collagen</term>
<term>Elastin</term>
<term>Hyaluronic Acid</term>
<term>Microfilament Proteins</term>
<term>Oligosaccharides</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Extracellular Matrix</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Aorte</term>
<term>Muscles lisses vasculaires</term>
<term>Myocytes du muscle lisse</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Aorta</term>
<term>Muscle, Smooth, Vascular</term>
<term>Myocytes, Smooth Muscle</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Myocytes, Smooth Muscle</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Extracellular Matrix</term>
<term>Myocytes, Smooth Muscle</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Guided Tissue Regeneration</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acide hyaluronique</term>
<term>Collagène</term>
<term>Matrice extracellulaire</term>
<term>Myocytes du muscle lisse</term>
<term>Oligosaccharides</term>
<term>Protéines des microfilaments</term>
<term>Élastine</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acide hyaluronique</term>
<term>Oligosaccharides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Hyaluronic Acid</term>
<term>Oligosaccharides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Collagen</term>
<term>Elastin</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Proliferation</term>
<term>Cells, Cultured</term>
<term>Fibrillins</term>
<term>Rats</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Acide hyaluronique</term>
<term>Animaux</term>
<term>Cellules cultivées</term>
<term>Collagène</term>
<term>Fibrillines</term>
<term>Matrice extracellulaire</term>
<term>Myocytes du muscle lisse</term>
<term>Oligosaccharides</term>
<term>Prolifération cellulaire</term>
<term>Rats</term>
<term>Régénération tissulaire guidée</term>
<term>Élastine</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Prior studies suggest that hyaluronan (HA), a glycosaminoglycan, may upregulate innately poor elastin matrix synthesis by adult vascular smooth muscle cells (SMCs). HA scaffolds could thus be useful to regenerate damaged vascular elastin. In an earlier study, we established that the elastogenic effects of non-oligomeric HA are fragment size- and/or dose-specific. We currently investigate the pro-elastogenic effects of exogenous HA oligomers on rat aortic smooth muscle cells (RASMCs). RASMCs were cultured with pure HA oligomers (4-mers) and mixtures (4-8mers) obtained by enzymatic digestion of long-chain HA (MW approximately 2000kDa). Polyacrylamide gel electrophoresis (PAGE)/Matrix Assisted Laser Desorption/Ionization Spectroscopy Time-Of-Flight Analysis (MALDI-TOF) showed HA digestates to contain a mixture of 4-8mers with a predominance of 4-mers (75+/-0.4% w/w). Cell layers supplemented with both pure HA 4-mers or oligomer mixtures showed proliferation levels similar to non-HA controls over 21 days of culture. Pure 4-mers and oligomer mixtures enhanced DNA-normalized output of tropoelastin by 1.6 and 1.8 times, respectively, and that of matrix elastin by approximately 2.7 times relative to controls. Sodium dodecyl sulfate (SDS)-PAGE/Western Blot and a desmosine assay semi-quantitatively confirmed the observed biochemical trends for tropoelastin and matrix elastin, respectively. HA oligomers induced enhanced synthesis of the elastin crosslinker, desmosine, and appeared to stabilize the elastin matrix by suppression of elastin-laminin receptor (ELR) activity relative to controls. Transmission electron micrographs (TEMs) showed elastin deposits within oligomer-supplemented cultures to be distinct, longitudinally oriented, aggregating fibrils, and clumps, and to be less abundant and mostly amorphous in controls. HA oligomers preserved normal fibrillin-mediated elastin matrix deposition. Results suggest that HA oligos are highly pro-elastogenic, promote elastin fibril formation, and stabilize elastin matrix and may thus be usefully incorporated into scaffolds for guided elastin regeneration.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16899292</PMID>
<DateCompleted><Year>2007</Year>
<Month>03</Month>
<Day>15</Day>
</DateCompleted>
<DateRevised><Year>2016</Year>
<Month>11</Month>
<Day>24</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0142-9612</ISSN>
<JournalIssue CitedMedium="Print"><Volume>27</Volume>
<Issue>33</Issue>
<PubDate><Year>2006</Year>
<Month>Nov</Month>
</PubDate>
</JournalIssue>
<Title>Biomaterials</Title>
<ISOAbbreviation>Biomaterials</ISOAbbreviation>
</Journal>
<ArticleTitle>Elastogenic effects of exogenous hyaluronan oligosaccharides on vascular smooth muscle cells.</ArticleTitle>
<Pagination><MedlinePgn>5698-707</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Prior studies suggest that hyaluronan (HA), a glycosaminoglycan, may upregulate innately poor elastin matrix synthesis by adult vascular smooth muscle cells (SMCs). HA scaffolds could thus be useful to regenerate damaged vascular elastin. In an earlier study, we established that the elastogenic effects of non-oligomeric HA are fragment size- and/or dose-specific. We currently investigate the pro-elastogenic effects of exogenous HA oligomers on rat aortic smooth muscle cells (RASMCs). RASMCs were cultured with pure HA oligomers (4-mers) and mixtures (4-8mers) obtained by enzymatic digestion of long-chain HA (MW approximately 2000kDa). Polyacrylamide gel electrophoresis (PAGE)/Matrix Assisted Laser Desorption/Ionization Spectroscopy Time-Of-Flight Analysis (MALDI-TOF) showed HA digestates to contain a mixture of 4-8mers with a predominance of 4-mers (75+/-0.4% w/w). Cell layers supplemented with both pure HA 4-mers or oligomer mixtures showed proliferation levels similar to non-HA controls over 21 days of culture. Pure 4-mers and oligomer mixtures enhanced DNA-normalized output of tropoelastin by 1.6 and 1.8 times, respectively, and that of matrix elastin by approximately 2.7 times relative to controls. Sodium dodecyl sulfate (SDS)-PAGE/Western Blot and a desmosine assay semi-quantitatively confirmed the observed biochemical trends for tropoelastin and matrix elastin, respectively. HA oligomers induced enhanced synthesis of the elastin crosslinker, desmosine, and appeared to stabilize the elastin matrix by suppression of elastin-laminin receptor (ELR) activity relative to controls. Transmission electron micrographs (TEMs) showed elastin deposits within oligomer-supplemented cultures to be distinct, longitudinally oriented, aggregating fibrils, and clumps, and to be less abundant and mostly amorphous in controls. HA oligomers preserved normal fibrillin-mediated elastin matrix deposition. Results suggest that HA oligos are highly pro-elastogenic, promote elastin fibril formation, and stabilize elastin matrix and may thus be usefully incorporated into scaffolds for guided elastin regeneration.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Joddar</LastName>
<ForeName>Binata</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Department of Bioengineering, Clemson University, Charleston, SC 29425, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ramamurthi</LastName>
<ForeName>Anand</ForeName>
<Initials>A</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>CO6 RR018823</GrantID>
<Acronym>CO</Acronym>
<Agency>NCI NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2006</Year>
<Month>08</Month>
<Day>08</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>Biomaterials</MedlineTA>
<NlmUniqueID>8100316</NlmUniqueID>
<ISSNLinking>0142-9612</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000071837">Fibrillins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008840">Microfilament Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D009844">Oligosaccharides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9004-61-9</RegistryNumber>
<NameOfSubstance UI="D006820">Hyaluronic Acid</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9007-34-5</RegistryNumber>
<NameOfSubstance UI="D003094">Collagen</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9007-58-3</RegistryNumber>
<NameOfSubstance UI="D004549">Elastin</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001011" MajorTopicYN="N">Aorta</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D049109" MajorTopicYN="N">Cell Proliferation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003094" MajorTopicYN="N">Collagen</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004549" MajorTopicYN="N">Elastin</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
<QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005109" MajorTopicYN="N">Extracellular Matrix</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000071837" MajorTopicYN="N">Fibrillins</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D048091" MajorTopicYN="N">Guided Tissue Regeneration</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006820" MajorTopicYN="N">Hyaluronic Acid</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008840" MajorTopicYN="N">Microfilament Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009131" MajorTopicYN="N">Muscle, Smooth, Vascular</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032389" MajorTopicYN="N">Myocytes, Smooth Muscle</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009844" MajorTopicYN="N">Oligosaccharides</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2006</Year>
<Month>05</Month>
<Day>24</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2006</Year>
<Month>07</Month>
<Day>20</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2006</Year>
<Month>8</Month>
<Day>11</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2007</Year>
<Month>3</Month>
<Day>16</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2006</Year>
<Month>8</Month>
<Day>11</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16899292</ArticleId>
<ArticleId IdType="pii">S0142-9612(06)00644-2</ArticleId>
<ArticleId IdType="doi">10.1016/j.biomaterials.2006.07.020</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Caroline du Sud</li>
</region>
</list>
<tree><noCountry><name sortKey="Ramamurthi, Anand" sort="Ramamurthi, Anand" uniqKey="Ramamurthi A" first="Anand" last="Ramamurthi">Anand Ramamurthi</name>
</noCountry>
<country name="États-Unis"><region name="Caroline du Sud"><name sortKey="Joddar, Binata" sort="Joddar, Binata" uniqKey="Joddar B" first="Binata" last="Joddar">Binata Joddar</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002134 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 002134 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= PubMed |étape= Checkpoint |type= RBID |clé= pubmed:16899292 |texte= Elastogenic effects of exogenous hyaluronan oligosaccharides on vascular smooth muscle cells. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:16899292" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |