Estrogens exert a rapid apoptotic action in anterior pituitary cells.
Identifieur interne : 001F14 ( PubMed/Checkpoint ); précédent : 001F13; suivant : 001F15Estrogens exert a rapid apoptotic action in anterior pituitary cells.
Auteurs : S. Zárate [Argentine] ; G. Jaita ; V. Zaldivar ; D B Radl ; G. Eijo ; J. Ferraris ; D. Pisera ; A. SeilicovichSource :
- American journal of physiology. Endocrinology and metabolism [ 0193-1849 ] ; 2009.
Descripteurs français
- KwdFr :
- Adénohypophyse (), Adénohypophyse (métabolisme), Adénohypophyse (physiologie), Animaux, Apoptose (), Cellules cultivées, Facteurs temps, Femelle, Hormone de croissance (métabolisme), Oestradiol (pharmacologie), Oestrogènes (pharmacologie), Oestrènes (pharmacologie), Prolactine (métabolisme), Rat Wistar, Rats, Récepteurs des oestrogènes (métabolisme).
- MESH :
- métabolisme : Adénohypophyse, Hormone de croissance, Prolactine, Récepteurs des oestrogènes.
- pharmacologie : Oestradiol, Oestrogènes, Oestrènes.
- physiologie : Adénohypophyse.
- Adénohypophyse, Animaux, Apoptose, Cellules cultivées, Facteurs temps, Femelle, Rat Wistar, Rats.
English descriptors
- KwdEn :
- Animals, Apoptosis (drug effects), Cells, Cultured, Estradiol (pharmacology), Estrenes (pharmacology), Estrogens (pharmacology), Female, Growth Hormone (metabolism), Pituitary Gland, Anterior (drug effects), Pituitary Gland, Anterior (metabolism), Pituitary Gland, Anterior (physiology), Prolactin (metabolism), Rats, Rats, Wistar, Receptors, Estrogen (metabolism), Time Factors.
- MESH :
- chemical , metabolism : Growth Hormone, Prolactin, Receptors, Estrogen.
- chemical , pharmacology : Estradiol, Estrenes, Estrogens.
- drug effects : Apoptosis, Pituitary Gland, Anterior.
- metabolism : Pituitary Gland, Anterior.
- physiology : Pituitary Gland, Anterior.
- Animals, Cells, Cultured, Female, Rats, Rats, Wistar, Time Factors.
Abstract
It is now accepted that estrogens not only stimulate lactotrope proliferation but also sensitize anterior pituitary cells to proapoptotic stimuli. In addition to their classical mechanism of action through binding to intracellular estrogen receptors (ERs), there is increasing evidence that estrogens exert rapid actions mediated by cell membrane-localized ERs (mERs). In the present study, we examined the involvement of membrane-initiated steroid signaling in the proapoptotic action of estradiol in primary cultures of anterior pituitary cells from ovariectomized rats by using estren, a synthetic estrogen with no effect on classical transcription and a cell-impermeable 17beta-estradiol conjugate (E2-BSA). Both compounds induced cell death of anterior pituitary cells after 60 min of incubation as assessed by flow cytometry and the [3-(4,5-dimethylthiazol-2-yl)]-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. Estren, E2, and E2-BSA induced apoptosis of lactotropes and somatotropes as evaluated by the deoxynucleotidyltransferase-mediated dUTP nick end-labeling assay and immunodetection of prolactin (PRL) and growth hormone (GH). The proapoptotic effect of E2-BSA was abrogated by ICI-182,780, an antagonist of ERs. The expression of membrane-associated ERalpha was observed in PRL- and GH-bearing cells. Our results indicate that estradiol is able to exert a rapid apoptotic action in anterior pituitary cells, especially lactotropes and somatotropes, by a mechanism triggered by mERs. This mechanism could be involved in anterior pituitary cell turnover.
DOI: 10.1152/ajpendo.90785.2008
PubMed: 19158323
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:19158323Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Estrogens exert a rapid apoptotic action in anterior pituitary cells.</title><author><name sortKey="Zarate, S" sort="Zarate, S" uniqKey="Zarate S" first="S" last="Zárate">S. Zárate</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, piso 10, Buenos Aires, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Instituto de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, piso 10, Buenos Aires</wicri:regionArea><wicri:noRegion>Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Jaita, G" sort="Jaita, G" uniqKey="Jaita G" first="G" last="Jaita">G. Jaita</name></author><author><name sortKey="Zaldivar, V" sort="Zaldivar, V" uniqKey="Zaldivar V" first="V" last="Zaldivar">V. Zaldivar</name></author><author><name sortKey="Radl, D B" sort="Radl, D B" uniqKey="Radl D" first="D B" last="Radl">D B Radl</name></author><author><name sortKey="Eijo, G" sort="Eijo, G" uniqKey="Eijo G" first="G" last="Eijo">G. Eijo</name></author><author><name sortKey="Ferraris, J" sort="Ferraris, J" uniqKey="Ferraris J" first="J" last="Ferraris">J. Ferraris</name></author><author><name sortKey="Pisera, D" sort="Pisera, D" uniqKey="Pisera D" first="D" last="Pisera">D. Pisera</name></author><author><name sortKey="Seilicovich, A" sort="Seilicovich, A" uniqKey="Seilicovich A" first="A" last="Seilicovich">A. Seilicovich</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19158323</idno><idno type="pmid">19158323</idno><idno type="doi">10.1152/ajpendo.90785.2008</idno><idno type="wicri:Area/PubMed/Corpus">002037</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002037</idno><idno type="wicri:Area/PubMed/Curation">002037</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002037</idno><idno type="wicri:Area/PubMed/Checkpoint">001F14</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001F14</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Estrogens exert a rapid apoptotic action in anterior pituitary cells.</title><author><name sortKey="Zarate, S" sort="Zarate, S" uniqKey="Zarate S" first="S" last="Zárate">S. Zárate</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, piso 10, Buenos Aires, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Instituto de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, piso 10, Buenos Aires</wicri:regionArea><wicri:noRegion>Buenos Aires</wicri:noRegion></affiliation></author><author><name sortKey="Jaita, G" sort="Jaita, G" uniqKey="Jaita G" first="G" last="Jaita">G. Jaita</name></author><author><name sortKey="Zaldivar, V" sort="Zaldivar, V" uniqKey="Zaldivar V" first="V" last="Zaldivar">V. Zaldivar</name></author><author><name sortKey="Radl, D B" sort="Radl, D B" uniqKey="Radl D" first="D B" last="Radl">D B Radl</name></author><author><name sortKey="Eijo, G" sort="Eijo, G" uniqKey="Eijo G" first="G" last="Eijo">G. Eijo</name></author><author><name sortKey="Ferraris, J" sort="Ferraris, J" uniqKey="Ferraris J" first="J" last="Ferraris">J. Ferraris</name></author><author><name sortKey="Pisera, D" sort="Pisera, D" uniqKey="Pisera D" first="D" last="Pisera">D. Pisera</name></author><author><name sortKey="Seilicovich, A" sort="Seilicovich, A" uniqKey="Seilicovich A" first="A" last="Seilicovich">A. Seilicovich</name></author></analytic><series><title level="j">American journal of physiology. Endocrinology and metabolism</title><idno type="ISSN">0193-1849</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Apoptosis (drug effects)</term><term>Cells, Cultured</term><term>Estradiol (pharmacology)</term><term>Estrenes (pharmacology)</term><term>Estrogens (pharmacology)</term><term>Female</term><term>Growth Hormone (metabolism)</term><term>Pituitary Gland, Anterior (drug effects)</term><term>Pituitary Gland, Anterior (metabolism)</term><term>Pituitary Gland, Anterior (physiology)</term><term>Prolactin (metabolism)</term><term>Rats</term><term>Rats, Wistar</term><term>Receptors, Estrogen (metabolism)</term><term>Time Factors</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adénohypophyse ()</term><term>Adénohypophyse (métabolisme)</term><term>Adénohypophyse (physiologie)</term><term>Animaux</term><term>Apoptose ()</term><term>Cellules cultivées</term><term>Facteurs temps</term><term>Femelle</term><term>Hormone de croissance (métabolisme)</term><term>Oestradiol (pharmacologie)</term><term>Oestrogènes (pharmacologie)</term><term>Oestrènes (pharmacologie)</term><term>Prolactine (métabolisme)</term><term>Rat Wistar</term><term>Rats</term><term>Récepteurs des oestrogènes (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Growth Hormone</term><term>Prolactin</term><term>Receptors, Estrogen</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Estradiol</term><term>Estrenes</term><term>Estrogens</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Apoptosis</term><term>Pituitary Gland, Anterior</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Pituitary Gland, Anterior</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Adénohypophyse</term><term>Hormone de croissance</term><term>Prolactine</term><term>Récepteurs des oestrogènes</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Oestradiol</term><term>Oestrogènes</term><term>Oestrènes</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Adénohypophyse</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Pituitary Gland, Anterior</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Female</term><term>Rats</term><term>Rats, Wistar</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adénohypophyse</term><term>Animaux</term><term>Apoptose</term><term>Cellules cultivées</term><term>Facteurs temps</term><term>Femelle</term><term>Rat Wistar</term><term>Rats</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">It is now accepted that estrogens not only stimulate lactotrope proliferation but also sensitize anterior pituitary cells to proapoptotic stimuli. In addition to their classical mechanism of action through binding to intracellular estrogen receptors (ERs), there is increasing evidence that estrogens exert rapid actions mediated by cell membrane-localized ERs (mERs). In the present study, we examined the involvement of membrane-initiated steroid signaling in the proapoptotic action of estradiol in primary cultures of anterior pituitary cells from ovariectomized rats by using estren, a synthetic estrogen with no effect on classical transcription and a cell-impermeable 17beta-estradiol conjugate (E2-BSA). Both compounds induced cell death of anterior pituitary cells after 60 min of incubation as assessed by flow cytometry and the [3-(4,5-dimethylthiazol-2-yl)]-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. Estren, E2, and E2-BSA induced apoptosis of lactotropes and somatotropes as evaluated by the deoxynucleotidyltransferase-mediated dUTP nick end-labeling assay and immunodetection of prolactin (PRL) and growth hormone (GH). The proapoptotic effect of E2-BSA was abrogated by ICI-182,780, an antagonist of ERs. The expression of membrane-associated ERalpha was observed in PRL- and GH-bearing cells. Our results indicate that estradiol is able to exert a rapid apoptotic action in anterior pituitary cells, especially lactotropes and somatotropes, by a mechanism triggered by mERs. This mechanism could be involved in anterior pituitary cell turnover.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19158323</PMID><DateCompleted><Year>2009</Year><Month>05</Month><Day>21</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0193-1849</ISSN><JournalIssue CitedMedium="Print"><Volume>296</Volume><Issue>4</Issue><PubDate><Year>2009</Year><Month>Apr</Month></PubDate></JournalIssue><Title>American journal of physiology. Endocrinology and metabolism</Title><ISOAbbreviation>Am. J. Physiol. Endocrinol. Metab.</ISOAbbreviation></Journal><ArticleTitle>Estrogens exert a rapid apoptotic action in anterior pituitary cells.</ArticleTitle><Pagination><MedlinePgn>E664-71</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1152/ajpendo.90785.2008</ELocationID><Abstract><AbstractText>It is now accepted that estrogens not only stimulate lactotrope proliferation but also sensitize anterior pituitary cells to proapoptotic stimuli. In addition to their classical mechanism of action through binding to intracellular estrogen receptors (ERs), there is increasing evidence that estrogens exert rapid actions mediated by cell membrane-localized ERs (mERs). In the present study, we examined the involvement of membrane-initiated steroid signaling in the proapoptotic action of estradiol in primary cultures of anterior pituitary cells from ovariectomized rats by using estren, a synthetic estrogen with no effect on classical transcription and a cell-impermeable 17beta-estradiol conjugate (E2-BSA). Both compounds induced cell death of anterior pituitary cells after 60 min of incubation as assessed by flow cytometry and the [3-(4,5-dimethylthiazol-2-yl)]-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. Estren, E2, and E2-BSA induced apoptosis of lactotropes and somatotropes as evaluated by the deoxynucleotidyltransferase-mediated dUTP nick end-labeling assay and immunodetection of prolactin (PRL) and growth hormone (GH). The proapoptotic effect of E2-BSA was abrogated by ICI-182,780, an antagonist of ERs. The expression of membrane-associated ERalpha was observed in PRL- and GH-bearing cells. Our results indicate that estradiol is able to exert a rapid apoptotic action in anterior pituitary cells, especially lactotropes and somatotropes, by a mechanism triggered by mERs. This mechanism could be involved in anterior pituitary cell turnover.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zárate</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Instituto de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, piso 10, Buenos Aires, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jaita</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Zaldivar</LastName><ForeName>V</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Radl</LastName><ForeName>D B</ForeName><Initials>DB</Initials></Author><Author ValidYN="Y"><LastName>Eijo</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Ferraris</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Pisera</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Seilicovich</LastName><ForeName>A</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>01</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Am J Physiol Endocrinol Metab</MedlineTA><NlmUniqueID>100901226</NlmUniqueID><ISSNLinking>0193-1849</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C468666">4-estren-3,17-diol</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004963">Estrenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004967">Estrogens</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011960">Receptors, Estrogen</NameOfSubstance></Chemical><Chemical><RegistryNumber>4TI98Z838E</RegistryNumber><NameOfSubstance UI="D004958">Estradiol</NameOfSubstance></Chemical><Chemical><RegistryNumber>9002-62-4</RegistryNumber><NameOfSubstance UI="D011388">Prolactin</NameOfSubstance></Chemical><Chemical><RegistryNumber>9002-72-6</RegistryNumber><NameOfSubstance UI="D013006">Growth Hormone</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017209" MajorTopicYN="N">Apoptosis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004958" MajorTopicYN="N">Estradiol</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004963" MajorTopicYN="N">Estrenes</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004967" MajorTopicYN="N">Estrogens</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013006" MajorTopicYN="N">Growth Hormone</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010903" MajorTopicYN="N">Pituitary Gland, Anterior</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011388" MajorTopicYN="N">Prolactin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017208" MajorTopicYN="N">Rats, Wistar</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011960" MajorTopicYN="N">Receptors, Estrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>1</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>1</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>5</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19158323</ArticleId><ArticleId IdType="pii">90785.2008</ArticleId><ArticleId IdType="doi">10.1152/ajpendo.90785.2008</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Argentine</li></country></list><tree><noCountry><name sortKey="Eijo, G" sort="Eijo, G" uniqKey="Eijo G" first="G" last="Eijo">G. Eijo</name><name sortKey="Ferraris, J" sort="Ferraris, J" uniqKey="Ferraris J" first="J" last="Ferraris">J. Ferraris</name><name sortKey="Jaita, G" sort="Jaita, G" uniqKey="Jaita G" first="G" last="Jaita">G. Jaita</name><name sortKey="Pisera, D" sort="Pisera, D" uniqKey="Pisera D" first="D" last="Pisera">D. Pisera</name><name sortKey="Radl, D B" sort="Radl, D B" uniqKey="Radl D" first="D B" last="Radl">D B Radl</name><name sortKey="Seilicovich, A" sort="Seilicovich, A" uniqKey="Seilicovich A" first="A" last="Seilicovich">A. Seilicovich</name><name sortKey="Zaldivar, V" sort="Zaldivar, V" uniqKey="Zaldivar V" first="V" last="Zaldivar">V. Zaldivar</name></noCountry><country name="Argentine"><noRegion><name sortKey="Zarate, S" sort="Zarate, S" uniqKey="Zarate S" first="S" last="Zárate">S. Zárate</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001F14 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 001F14 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:19158323
|texte= Estrogens exert a rapid apoptotic action in anterior pituitary cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:19158323" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |