Minimizing off-target signals in RNA fluorescent in situ hybridization.
Identifieur interne : 001E30 ( PubMed/Checkpoint ); précédent : 001E29; suivant : 001E31Minimizing off-target signals in RNA fluorescent in situ hybridization.
Auteurs : Aaron Arvey [États-Unis] ; Anita Hermann ; Cheryl C. Hsia ; Eugene Ie ; Yoav Freund ; William McginnisSource :
- Nucleic acids research [ 1362-4962 ] ; 2010.
Descripteurs français
- KwdFr :
- ARN messager (), ARN messager (analyse), Animaux, Drosophila melanogaster (embryologie), Drosophila melanogaster (génétique), Embryon non mammalien (), Facteurs de transcription (génétique), Protéines de Drosophila (génétique), Protéines nucléaires (génétique), Sondes d'ARN (), Séquences répétées d'acides nucléiques, Technique FISH ().
- MESH :
- analyse : ARN messager.
- embryologie : Drosophila melanogaster.
- génétique : Drosophila melanogaster, Facteurs de transcription, Protéines de Drosophila, Protéines nucléaires.
- ARN messager, Animaux, Embryon non mammalien, Sondes d'ARN, Séquences répétées d'acides nucléiques, Technique FISH.
English descriptors
- KwdEn :
- Animals, Drosophila Proteins (genetics), Drosophila melanogaster (embryology), Drosophila melanogaster (genetics), Embryo, Nonmammalian (chemistry), In Situ Hybridization, Fluorescence (methods), Nuclear Proteins (genetics), RNA Probes (chemistry), RNA, Messenger (analysis), RNA, Messenger (chemistry), Repetitive Sequences, Nucleic Acid, Transcription Factors (genetics).
- MESH :
- chemical , analysis : RNA, Messenger.
- chemical , chemistry : RNA Probes, RNA, Messenger.
- chemical , genetics : Drosophila Proteins, Nuclear Proteins, Transcription Factors.
- chemistry : Embryo, Nonmammalian.
- embryology : Drosophila melanogaster.
- genetics : Drosophila melanogaster.
- methods : In Situ Hybridization, Fluorescence.
- Animals, Repetitive Sequences, Nucleic Acid.
Abstract
Fluorescent in situ hybridization (FISH) techniques are becoming extremely sensitive, to the point where individual RNA or DNA molecules can be detected with small probes. At this level of sensitivity, the elimination of 'off-target' hybridization is of crucial importance, but typical probes used for RNA and DNA FISH contain sequences repeated elsewhere in the genome. We find that very short (e.g. 20 nt) perfect repeated sequences within much longer probes (e.g. 350-1500 nt) can produce significant off-target signals. The extent of noise is surprising given the long length of the probes and the short length of non-specific regions. When we removed the small regions of repeated sequence from either short or long probes, we find that the signal-to-noise ratio is increased by orders of magnitude, putting us in a regime where fluorescent signals can be considered to be a quantitative measure of target transcript numbers. As the majority of genes in complex organisms contain repeated k-mers, we provide genome-wide annotations of k-mer-uniqueness at http://cbio.mskcc.org/ approximately aarvey/repeatmap.
DOI: 10.1093/nar/gkq042
PubMed: 20164092
Affiliations:
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Hsia</name></author><author><name sortKey="Ie, Eugene" sort="Ie, Eugene" uniqKey="Ie E" first="Eugene" last="Ie">Eugene Ie</name></author><author><name sortKey="Freund, Yoav" sort="Freund, Yoav" uniqKey="Freund Y" first="Yoav" last="Freund">Yoav Freund</name></author><author><name sortKey="Mcginnis, William" sort="Mcginnis, William" uniqKey="Mcginnis W" first="William" last="Mcginnis">William Mcginnis</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20164092</idno><idno type="pmid">20164092</idno><idno type="doi">10.1093/nar/gkq042</idno><idno type="wicri:Area/PubMed/Corpus">001F66</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001F66</idno><idno type="wicri:Area/PubMed/Curation">001F66</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001F66</idno><idno type="wicri:Area/PubMed/Checkpoint">001E30</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001E30</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Minimizing off-target signals in RNA fluorescent in situ hybridization.</title><author><name sortKey="Arvey, Aaron" sort="Arvey, Aaron" uniqKey="Arvey A" first="Aaron" last="Arvey">Aaron Arvey</name><affiliation wicri:level="2"><nlm:affiliation>Computational and Systems Biology Center, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Computational and Systems Biology Center, Memorial Sloan-Kettering Cancer Center, New York, NY 10065</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Hermann, Anita" sort="Hermann, Anita" uniqKey="Hermann A" first="Anita" last="Hermann">Anita Hermann</name></author><author><name sortKey="Hsia, Cheryl C" sort="Hsia, Cheryl C" uniqKey="Hsia C" first="Cheryl C" last="Hsia">Cheryl C. Hsia</name></author><author><name sortKey="Ie, Eugene" sort="Ie, Eugene" uniqKey="Ie E" first="Eugene" last="Ie">Eugene Ie</name></author><author><name sortKey="Freund, Yoav" sort="Freund, Yoav" uniqKey="Freund Y" first="Yoav" last="Freund">Yoav Freund</name></author><author><name sortKey="Mcginnis, William" sort="Mcginnis, William" uniqKey="Mcginnis W" first="William" last="Mcginnis">William Mcginnis</name></author></analytic><series><title level="j">Nucleic acids research</title><idno type="eISSN">1362-4962</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Drosophila Proteins (genetics)</term><term>Drosophila melanogaster (embryology)</term><term>Drosophila melanogaster (genetics)</term><term>Embryo, Nonmammalian (chemistry)</term><term>In Situ Hybridization, Fluorescence (methods)</term><term>Nuclear Proteins (genetics)</term><term>RNA Probes (chemistry)</term><term>RNA, Messenger (analysis)</term><term>RNA, Messenger (chemistry)</term><term>Repetitive Sequences, Nucleic Acid</term><term>Transcription Factors (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager ()</term><term>ARN messager (analyse)</term><term>Animaux</term><term>Drosophila melanogaster (embryologie)</term><term>Drosophila melanogaster (génétique)</term><term>Embryon non mammalien ()</term><term>Facteurs de transcription (génétique)</term><term>Protéines de Drosophila (génétique)</term><term>Protéines nucléaires (génétique)</term><term>Sondes d'ARN ()</term><term>Séquences répétées d'acides nucléiques</term><term>Technique FISH ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA Probes</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Drosophila Proteins</term><term>Nuclear Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN messager</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Embryo, Nonmammalian</term></keywords><keywords scheme="MESH" qualifier="embryologie" xml:lang="fr"><term>Drosophila melanogaster</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Drosophila melanogaster</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Drosophila melanogaster</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Drosophila melanogaster</term><term>Facteurs de transcription</term><term>Protéines de Drosophila</term><term>Protéines nucléaires</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>In Situ Hybridization, Fluorescence</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Repetitive Sequences, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN messager</term><term>Animaux</term><term>Embryon non mammalien</term><term>Sondes d'ARN</term><term>Séquences répétées d'acides nucléiques</term><term>Technique FISH</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fluorescent in situ hybridization (FISH) techniques are becoming extremely sensitive, to the point where individual RNA or DNA molecules can be detected with small probes. At this level of sensitivity, the elimination of 'off-target' hybridization is of crucial importance, but typical probes used for RNA and DNA FISH contain sequences repeated elsewhere in the genome. We find that very short (e.g. 20 nt) perfect repeated sequences within much longer probes (e.g. 350-1500 nt) can produce significant off-target signals. The extent of noise is surprising given the long length of the probes and the short length of non-specific regions. When we removed the small regions of repeated sequence from either short or long probes, we find that the signal-to-noise ratio is increased by orders of magnitude, putting us in a regime where fluorescent signals can be considered to be a quantitative measure of target transcript numbers. As the majority of genes in complex organisms contain repeated k-mers, we provide genome-wide annotations of k-mer-uniqueness at http://cbio.mskcc.org/ approximately aarvey/repeatmap.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20164092</PMID><DateCompleted><Year>2010</Year><Month>06</Month><Day>22</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1362-4962</ISSN><JournalIssue CitedMedium="Internet"><Volume>38</Volume><Issue>10</Issue><PubDate><Year>2010</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Nucleic acids research</Title><ISOAbbreviation>Nucleic Acids Res.</ISOAbbreviation></Journal><ArticleTitle>Minimizing off-target signals in RNA fluorescent in situ hybridization.</ArticleTitle><Pagination><MedlinePgn>e115</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/nar/gkq042</ELocationID><Abstract><AbstractText>Fluorescent in situ hybridization (FISH) techniques are becoming extremely sensitive, to the point where individual RNA or DNA molecules can be detected with small probes. At this level of sensitivity, the elimination of 'off-target' hybridization is of crucial importance, but typical probes used for RNA and DNA FISH contain sequences repeated elsewhere in the genome. We find that very short (e.g. 20 nt) perfect repeated sequences within much longer probes (e.g. 350-1500 nt) can produce significant off-target signals. The extent of noise is surprising given the long length of the probes and the short length of non-specific regions. When we removed the small regions of repeated sequence from either short or long probes, we find that the signal-to-noise ratio is increased by orders of magnitude, putting us in a regime where fluorescent signals can be considered to be a quantitative measure of target transcript numbers. As the majority of genes in complex organisms contain repeated k-mers, we provide genome-wide annotations of k-mer-uniqueness at http://cbio.mskcc.org/ approximately aarvey/repeatmap.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Arvey</LastName><ForeName>Aaron</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Computational and Systems Biology Center, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hermann</LastName><ForeName>Anita</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Hsia</LastName><ForeName>Cheryl C</ForeName><Initials>CC</Initials></Author><Author ValidYN="Y"><LastName>Ie</LastName><ForeName>Eugene</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Freund</LastName><ForeName>Yoav</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>McGinnis</LastName><ForeName>William</ForeName><Initials>W</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R01 GM077197</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R37 HD028315</GrantID><Acronym>HD</Acronym><Agency>NICHD NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R37HD28315</GrantID><Acronym>HD</Acronym><Agency>NICHD NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>T32GM07240</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType></PublicationTypeList><ArticleDate 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MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>2</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>2</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>6</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20164092</ArticleId><ArticleId IdType="pii">gkq042</ArticleId><ArticleId IdType="doi">10.1093/nar/gkq042</ArticleId><ArticleId IdType="pmc">PMC2879521</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nucleic Acids Res. 2009 Jan;37(Database issue):D555-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18948289</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2008 Oct;5(10):877-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18806792</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2000 Nov 15;28(22):4552-7</Citation><ArticleIdList><ArticleId IdType="pubmed">11071945</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Enzymol. 2003;361:245-304</Citation><ArticleIdList><ArticleId IdType="pubmed">12624916</ArticleId></ArticleIdList></Reference><Reference><Citation>J Cell Sci. 2003 Jul 15;116(Pt 14):2833-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12808017</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2003 Oct;13(10):2306-15</Citation><ArticleIdList><ArticleId IdType="pubmed">12975312</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2004 Aug 6;305(5685):846</Citation><ArticleIdList><ArticleId IdType="pubmed">15297669</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1978 Dec;75(12):6125-9</Citation><ArticleIdList><ArticleId IdType="pubmed">216014</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1981 Jun 25;9(12):2819-40</Citation><ArticleIdList><ArticleId IdType="pubmed">6169002</ArticleId></ArticleIdList></Reference><Reference><Citation>Dev Biol. 1984 Feb;101(2):485-502</Citation><ArticleIdList><ArticleId IdType="pubmed">6692991</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 1987 Sep;117(1):51-60</Citation><ArticleIdList><ArticleId IdType="pubmed">3117618</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1990 Oct 5;215(3):403-10</Citation><ArticleIdList><ArticleId IdType="pubmed">2231712</ArticleId></ArticleIdList></Reference><Reference><Citation>Development. 1991 Feb;111(2):437-49</Citation><ArticleIdList><ArticleId IdType="pubmed">1680047</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1998 Apr 24;280(5363):585-90</Citation><ArticleIdList><ArticleId IdType="pubmed">9554849</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2005 Jul;71(7):3753-60</Citation><ArticleIdList><ArticleId IdType="pubmed">16000786</ArticleId></ArticleIdList></Reference><Reference><Citation>Gastroenterology. 2005 Aug;129(2):626-38</Citation><ArticleIdList><ArticleId IdType="pubmed">16083717</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6605-10</Citation><ArticleIdList><ArticleId IdType="pubmed">16636294</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2006 Oct 1;22(19):2437-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16740623</ArticleId></ArticleIdList></Reference><Reference><Citation>Cancer Res. 2008 Jul 1;68(13):4977-82</Citation><ArticleIdList><ArticleId IdType="pubmed">18593893</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2008;420:289-302</Citation><ArticleIdList><ArticleId IdType="pubmed">18641955</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2009;10(3):R25</Citation><ArticleIdList><ArticleId IdType="pubmed">19261174</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><noCountry><name sortKey="Freund, Yoav" sort="Freund, Yoav" uniqKey="Freund Y" first="Yoav" last="Freund">Yoav Freund</name><name sortKey="Hermann, Anita" sort="Hermann, Anita" uniqKey="Hermann A" first="Anita" last="Hermann">Anita Hermann</name><name sortKey="Hsia, Cheryl C" sort="Hsia, Cheryl C" uniqKey="Hsia C" first="Cheryl C" last="Hsia">Cheryl C. Hsia</name><name sortKey="Ie, Eugene" sort="Ie, Eugene" uniqKey="Ie E" first="Eugene" last="Ie">Eugene Ie</name><name sortKey="Mcginnis, William" sort="Mcginnis, William" uniqKey="Mcginnis W" first="William" last="Mcginnis">William Mcginnis</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Arvey, Aaron" sort="Arvey, Aaron" uniqKey="Arvey A" first="Aaron" last="Arvey">Aaron Arvey</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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