Short locked nucleic acid antisense oligonucleotides potently reduce apolipoprotein B mRNA and serum cholesterol in mice and non-human primates.
Identifieur interne : 001E19 ( PubMed/Checkpoint ); précédent : 001E18; suivant : 001E20Short locked nucleic acid antisense oligonucleotides potently reduce apolipoprotein B mRNA and serum cholesterol in mice and non-human primates.
Auteurs : Ellen Marie Straarup [Danemark] ; Niels Fisker ; Maj Hedtj Rn ; Marie W. Lindholm ; Christoph Rosenbohm ; Vibeke Aarup ; Henrik Frydenlund Hansen ; Henrik Rum ; Jens B Rode Hansen ; Troels KochSource :
- Nucleic acids research [ 1362-4962 ] ; 2010.
Descripteurs français
- KwdFr :
- ARN messager (métabolisme), Animaux, Apolipoprotéines B (génétique), Apolipoprotéines B (métabolisme), Autoradiographie, Cholestérol (sang), Femelle, Humains, Lignée cellulaire tumorale, Macaca fascicularis, Mésappariement de bases, Oligonucléotides (), Oligonucléotides (pharmacocinétique), Oligonucléotides (pharmacologie), Oligonucléotides antisens (), Oligonucléotides antisens (pharmacocinétique), Oligonucléotides antisens (pharmacologie), Souris, Souris de lignée C57BL.
- MESH :
- génétique : Apolipoprotéines B.
- métabolisme : ARN messager, Apolipoprotéines B.
- pharmacocinétique : Oligonucléotides, Oligonucléotides antisens.
- pharmacologie : Oligonucléotides, Oligonucléotides antisens.
- sang : Cholestérol.
- Animaux, Autoradiographie, Femelle, Humains, Lignée cellulaire tumorale, Macaca fascicularis, Mésappariement de bases, Oligonucléotides, Oligonucléotides antisens, Souris, Souris de lignée C57BL.
English descriptors
- KwdEn :
- Animals, Apolipoproteins B (genetics), Apolipoproteins B (metabolism), Autoradiography, Base Pair Mismatch, Cell Line, Tumor, Cholesterol (blood), Female, Humans, Macaca fascicularis, Mice, Mice, Inbred C57BL, Oligonucleotides (chemistry), Oligonucleotides (pharmacokinetics), Oligonucleotides (pharmacology), Oligonucleotides, Antisense (chemistry), Oligonucleotides, Antisense (pharmacokinetics), Oligonucleotides, Antisense (pharmacology), RNA, Messenger (metabolism).
- MESH :
- chemical , blood : Cholesterol.
- chemical , chemistry : Oligonucleotides, Oligonucleotides, Antisense.
- chemical , genetics : Apolipoproteins B.
- chemical , metabolism : Apolipoproteins B, RNA, Messenger.
- chemical , pharmacokinetics : Oligonucleotides, Oligonucleotides, Antisense.
- chemical , pharmacology : Oligonucleotides, Oligonucleotides, Antisense.
- Animals, Autoradiography, Base Pair Mismatch, Cell Line, Tumor, Female, Humans, Macaca fascicularis, Mice, Mice, Inbred C57BL.
Abstract
The potency and specificity of locked nucleic acid (LNA) antisense oligonucleotides was investigated as a function of length and affinity. The oligonucleotides were designed to target apolipoprotein B (apoB) and were investigated both in vitro and in vivo. The high affinity of LNA enabled the design of short antisense oligonucleotides (12- to 13-mers) that possessed high affinity and increased potency both in vitro and in vivo compared to longer oligonucleotides. The short LNA oligonucleotides were more target specific, and they exhibited the same biodistribution and tissue half-life as longer oligonucleotides. Pharmacology studies in both mice and non-human primates were conducted with a 13-mer LNA oligonucleotide against apoB, and the data showed that repeated dosing of the 13-mer at 1-2 mg/kg/week was sufficient to provide a significant and long lasting lowering of non-high-density lipoprotein (non-HDL) cholesterol without increasing serum liver toxicity markers. The data presented here show that oligonucleotide length as a parameter needs to be considered in the design of antisense oligonucleotide and that potent short oligonucleotides with sufficient target affinity can be generated using the LNA chemistry. Conclusively, we present a 13-mer LNA oligonucleotide with therapeutic potential that produce beneficial cholesterol lowering effect in non-human primates.
DOI: 10.1093/nar/gkq457
PubMed: 20615897
Affiliations:
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pubmed:20615897Le document en format XML
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The oligonucleotides were designed to target apolipoprotein B (apoB) and were investigated both in vitro and in vivo. The high affinity of LNA enabled the design of short antisense oligonucleotides (12- to 13-mers) that possessed high affinity and increased potency both in vitro and in vivo compared to longer oligonucleotides. The short LNA oligonucleotides were more target specific, and they exhibited the same biodistribution and tissue half-life as longer oligonucleotides. Pharmacology studies in both mice and non-human primates were conducted with a 13-mer LNA oligonucleotide against apoB, and the data showed that repeated dosing of the 13-mer at 1-2 mg/kg/week was sufficient to provide a significant and long lasting lowering of non-high-density lipoprotein (non-HDL) cholesterol without increasing serum liver toxicity markers. The data presented here show that oligonucleotide length as a parameter needs to be considered in the design of antisense oligonucleotide and that potent short oligonucleotides with sufficient target affinity can be generated using the LNA chemistry. Conclusively, we present a 13-mer LNA oligonucleotide with therapeutic potential that produce beneficial cholesterol lowering effect in non-human primates.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20615897</PMID><DateCompleted><Year>2011</Year><Month>01</Month><Day>04</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1362-4962</ISSN><JournalIssue CitedMedium="Internet"><Volume>38</Volume><Issue>20</Issue><PubDate><Year>2010</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Nucleic acids research</Title><ISOAbbreviation>Nucleic Acids Res.</ISOAbbreviation></Journal><ArticleTitle>Short locked nucleic acid antisense oligonucleotides potently reduce apolipoprotein B mRNA and serum cholesterol in mice and non-human primates.</ArticleTitle><Pagination><MedlinePgn>7100-11</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/nar/gkq457</ELocationID><Abstract><AbstractText>The potency and specificity of locked nucleic acid (LNA) antisense oligonucleotides was investigated as a function of length and affinity. The oligonucleotides were designed to target apolipoprotein B (apoB) and were investigated both in vitro and in vivo. The high affinity of LNA enabled the design of short antisense oligonucleotides (12- to 13-mers) that possessed high affinity and increased potency both in vitro and in vivo compared to longer oligonucleotides. The short LNA oligonucleotides were more target specific, and they exhibited the same biodistribution and tissue half-life as longer oligonucleotides. Pharmacology studies in both mice and non-human primates were conducted with a 13-mer LNA oligonucleotide against apoB, and the data showed that repeated dosing of the 13-mer at 1-2 mg/kg/week was sufficient to provide a significant and long lasting lowering of non-high-density lipoprotein (non-HDL) cholesterol without increasing serum liver toxicity markers. The data presented here show that oligonucleotide length as a parameter needs to be considered in the design of antisense oligonucleotide and that potent short oligonucleotides with sufficient target affinity can be generated using the LNA chemistry. Conclusively, we present a 13-mer LNA oligonucleotide with therapeutic potential that produce beneficial cholesterol lowering effect in non-human primates.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Straarup</LastName><ForeName>Ellen Marie</ForeName><Initials>EM</Initials><AffiliationInfo><Affiliation>Santaris Pharma A/S, Kogle Allé 6, DK-2970 Hørsholm, Denmark.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fisker</LastName><ForeName>Niels</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Hedtjärn</LastName><ForeName>Maj</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Lindholm</LastName><ForeName>Marie W</ForeName><Initials>MW</Initials></Author><Author ValidYN="Y"><LastName>Rosenbohm</LastName><ForeName>Christoph</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Aarup</LastName><ForeName>Vibeke</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Hansen</LastName><ForeName>Henrik Frydenlund</ForeName><Initials>HF</Initials></Author><Author ValidYN="Y"><LastName>Ørum</LastName><ForeName>Henrik</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Hansen</LastName><ForeName>Jens B Rode</ForeName><Initials>JB</Initials></Author><Author ValidYN="Y"><LastName>Koch</LastName><ForeName>Troels</ForeName><Initials>T</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>07</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nucleic Acids Res</MedlineTA><NlmUniqueID>0411011</NlmUniqueID><ISSNLinking>0305-1048</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001055">Apolipoproteins B</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009841">Oligonucleotides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016376">Oligonucleotides, Antisense</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C477371">locked nucleic acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>97C5T2UQ7J</RegistryNumber><NameOfSubstance UI="D002784">Cholesterol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001055" MajorTopicYN="N">Apolipoproteins B</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001345" MajorTopicYN="N">Autoradiography</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020137" MajorTopicYN="N">Base Pair Mismatch</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045744" MajorTopicYN="N">Cell Line, Tumor</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002784" MajorTopicYN="N">Cholesterol</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008252" MajorTopicYN="N">Macaca fascicularis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008810" MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009841" MajorTopicYN="N">Oligonucleotides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000493" MajorTopicYN="N">pharmacokinetics</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016376" MajorTopicYN="N">Oligonucleotides, Antisense</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000493" MajorTopicYN="N">pharmacokinetics</QualifierName><QualifierName UI="Q000494" 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Acids Res. 2002 May 1;30(9):1911-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11972327</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2004 Nov 11;432(7014):173-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15538359</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2002 Dec 1;30(23):5160-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12466540</ArticleId></ArticleIdList></Reference><Reference><Citation>J Lipid Res. 2003 Mar;44(3):470-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12562873</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2003 Jun 15;31(12):3185-93</Citation><ArticleIdList><ArticleId IdType="pubmed">12799446</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1041-3</Citation><ArticleIdList><ArticleId IdType="pubmed">14565339</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2003 Nov 1;31(21):6365-72</Citation><ArticleIdList><ArticleId IdType="pubmed">14576324</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):15206-11</Citation><ArticleIdList><ArticleId IdType="pubmed">14638944</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Endocrinol Metab. 2004 Apr;89(4):1849-57</Citation><ArticleIdList><ArticleId IdType="pubmed">15070955</ArticleId></ArticleIdList></Reference><Reference><Citation>Oligonucleotides. 2004;14(2):147-56</Citation><ArticleIdList><ArticleId IdType="pubmed">15294077</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Pharmacol. 1992 Feb;41(2):223-9</Citation><ArticleIdList><ArticleId IdType="pubmed">1371582</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 2004 Dec 17;578(3):285-90</Citation><ArticleIdList><ArticleId IdType="pubmed">15589834</ArticleId></ArticleIdList></Reference><Reference><Citation>Oligonucleotides. 2004;14(3):199-209</Citation><ArticleIdList><ArticleId IdType="pubmed">15625915</ArticleId></ArticleIdList></Reference><Reference><Citation>J Lipid Res. 2005 May;46(5):872-84</Citation><ArticleIdList><ArticleId IdType="pubmed">15716585</ArticleId></ArticleIdList></Reference><Reference><Citation>Chembiochem. 2005 Jun;6(6):1104-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15861430</ArticleId></ArticleIdList></Reference><Reference><Citation>Oligonucleotides. 2006 Spring;16(1):26-42</Citation><ArticleIdList><ArticleId IdType="pubmed">16584293</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2006 May 4;441(7089):111-4</Citation><ArticleIdList><ArticleId IdType="pubmed">16565705</ArticleId></ArticleIdList></Reference><Reference><Citation>Oligonucleotides. 2006 Summer;16(2):169-80</Citation><ArticleIdList><ArticleId IdType="pubmed">16764540</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Immunol. 2006 Jul;6(7):508-19</Citation><ArticleIdList><ArticleId IdType="pubmed">16778830</ArticleId></ArticleIdList></Reference><Reference><Citation>Circulation. 2006 Oct 17;114(16):1729-35</Citation><ArticleIdList><ArticleId IdType="pubmed">17030687</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2007;35(2):687-700</Citation><ArticleIdList><ArticleId IdType="pubmed">17182632</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Lipidol. 2007 Aug;18(4):397-400</Citation><ArticleIdList><ArticleId IdType="pubmed">17620855</ArticleId></ArticleIdList></Reference><Reference><Citation>BioDrugs. 2007;21(4):235-43</Citation><ArticleIdList><ArticleId IdType="pubmed">17628121</ArticleId></ArticleIdList></Reference><Reference><Citation>Vasc Health Risk Manag. 2007;3(4):491-502</Citation><ArticleIdList><ArticleId IdType="pubmed">17969379</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ther. 2008 Apr;16(4):734-40</Citation><ArticleIdList><ArticleId IdType="pubmed">18362929</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Pharmacokinet. 2009;48(1):39-50</Citation><ArticleIdList><ArticleId IdType="pubmed">19071883</ArticleId></ArticleIdList></Reference><Reference><Citation>J Med Chem. 2009 Jan 8;52(1):10-3</Citation><ArticleIdList><ArticleId IdType="pubmed">19086780</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Pharmacol. 2009 Mar 1;77(5):910-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19056355</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2010 Jan;38(1):e3</Citation><ArticleIdList><ArticleId IdType="pubmed">19854938</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2010 Apr 2;285(14):10583-90</Citation><ArticleIdList><ArticleId IdType="pubmed">20103594</ArticleId></ArticleIdList></Reference><Reference><Citation>J Lipid Res. 1993 Aug;34(8):1367-83</Citation><ArticleIdList><ArticleId IdType="pubmed">8409768</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 2002 Aug 6;41(31):9973-81</Citation><ArticleIdList><ArticleId IdType="pubmed">12146961</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Danemark</li></country></list><tree><noCountry><name sortKey=" Rum, Henrik" sort=" Rum, Henrik" uniqKey=" Rum H" first="Henrik" last=" Rum">Henrik Rum</name><name sortKey="Aarup, Vibeke" sort="Aarup, Vibeke" uniqKey="Aarup V" first="Vibeke" last="Aarup">Vibeke Aarup</name><name sortKey="Fisker, Niels" sort="Fisker, Niels" uniqKey="Fisker N" first="Niels" last="Fisker">Niels Fisker</name><name sortKey="Hansen, Henrik Frydenlund" sort="Hansen, Henrik Frydenlund" uniqKey="Hansen H" first="Henrik Frydenlund" last="Hansen">Henrik Frydenlund Hansen</name><name sortKey="Hansen, Jens B Rode" sort="Hansen, Jens B Rode" uniqKey="Hansen J" first="Jens B Rode" last="Hansen">Jens B Rode Hansen</name><name sortKey="Hedtj Rn, Maj" sort="Hedtj Rn, Maj" uniqKey="Hedtj Rn M" first="Maj" last="Hedtj Rn">Maj Hedtj Rn</name><name sortKey="Koch, Troels" sort="Koch, Troels" uniqKey="Koch T" first="Troels" last="Koch">Troels Koch</name><name sortKey="Lindholm, Marie W" sort="Lindholm, Marie W" uniqKey="Lindholm M" first="Marie W" last="Lindholm">Marie W. Lindholm</name><name sortKey="Rosenbohm, Christoph" sort="Rosenbohm, Christoph" uniqKey="Rosenbohm C" first="Christoph" last="Rosenbohm">Christoph Rosenbohm</name></noCountry><country name="Danemark"><noRegion><name sortKey="Straarup, Ellen Marie" sort="Straarup, Ellen Marie" uniqKey="Straarup E" first="Ellen Marie" last="Straarup">Ellen Marie Straarup</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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