RAD tag sequencing as a source of SNP markers in Cynara cardunculus L.
Identifieur interne : 001C45 ( PubMed/Checkpoint ); précédent : 001C44; suivant : 001C46RAD tag sequencing as a source of SNP markers in Cynara cardunculus L.
Auteurs : Davide Scaglione [Italie] ; Alberto Acquadro ; Ezio Portis ; Matteo Tirone ; Steven J. Knapp ; Sergio LanteriSource :
- BMC genomics [ 1471-2164 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Genetic Markers.
- chemical , metabolism : DNA Restriction Enzymes.
- genetics : Cynara.
- Contig Mapping, Gene Frequency, Genetic Linkage, Heterozygote, Polymorphism, Single Nucleotide, Sequence Analysis, DNA.
Abstract
The globe artichoke (Cynara cardunculus L. var. scolymus) genome is relatively poorly explored, especially compared to those of the other major Asteraceae crops sunflower and lettuce. No SNP markers are in the public domain. We have combined the recently developed restriction-site associated DNA (RAD) approach with the Illumina DNA sequencing platform to effect the rapid and mass discovery of SNP markers for C. cardunculus.
DOI: 10.1186/1471-2164-13-3
PubMed: 22214349
Affiliations:
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Plant Genetics and Breeding, University of Torino, via L. da Vinci44, 10095 Grugliasco (Torino)</wicri:regionArea><wicri:noRegion>10095 Grugliasco (Torino)</wicri:noRegion></affiliation></author><author><name sortKey="Acquadro, Alberto" sort="Acquadro, Alberto" uniqKey="Acquadro A" first="Alberto" last="Acquadro">Alberto Acquadro</name></author><author><name sortKey="Portis, Ezio" sort="Portis, Ezio" uniqKey="Portis E" first="Ezio" last="Portis">Ezio Portis</name></author><author><name sortKey="Tirone, Matteo" sort="Tirone, Matteo" uniqKey="Tirone M" first="Matteo" last="Tirone">Matteo Tirone</name></author><author><name sortKey="Knapp, Steven J" sort="Knapp, Steven J" uniqKey="Knapp S" first="Steven J" last="Knapp">Steven J. Knapp</name></author><author><name sortKey="Lanteri, Sergio" sort="Lanteri, Sergio" uniqKey="Lanteri S" first="Sergio" last="Lanteri">Sergio Lanteri</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22214349</idno><idno type="pmid">22214349</idno><idno type="doi">10.1186/1471-2164-13-3</idno><idno type="wicri:Area/PubMed/Corpus">001E21</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001E21</idno><idno type="wicri:Area/PubMed/Curation">001E21</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001E21</idno><idno type="wicri:Area/PubMed/Checkpoint">001C45</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001C45</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">RAD tag sequencing as a source of SNP markers in Cynara cardunculus L.</title><author><name sortKey="Scaglione, Davide" sort="Scaglione, Davide" uniqKey="Scaglione D" first="Davide" last="Scaglione">Davide Scaglione</name><affiliation wicri:level="1"><nlm:affiliation>Di.Va.P.R.A. Plant Genetics and Breeding, University of Torino, via L. da Vinci44, 10095 Grugliasco (Torino), Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Di.Va.P.R.A. Plant Genetics and Breeding, University of Torino, via L. da Vinci44, 10095 Grugliasco (Torino)</wicri:regionArea><wicri:noRegion>10095 Grugliasco (Torino)</wicri:noRegion></affiliation></author><author><name sortKey="Acquadro, Alberto" sort="Acquadro, Alberto" uniqKey="Acquadro A" first="Alberto" last="Acquadro">Alberto Acquadro</name></author><author><name sortKey="Portis, Ezio" sort="Portis, Ezio" uniqKey="Portis E" first="Ezio" last="Portis">Ezio Portis</name></author><author><name sortKey="Tirone, Matteo" sort="Tirone, Matteo" uniqKey="Tirone M" first="Matteo" last="Tirone">Matteo Tirone</name></author><author><name sortKey="Knapp, Steven J" sort="Knapp, Steven J" uniqKey="Knapp S" first="Steven J" last="Knapp">Steven J. Knapp</name></author><author><name sortKey="Lanteri, Sergio" sort="Lanteri, Sergio" uniqKey="Lanteri S" first="Sergio" last="Lanteri">Sergio Lanteri</name></author></analytic><series><title level="j">BMC genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Contig Mapping</term><term>Cynara (genetics)</term><term>DNA Restriction Enzymes (metabolism)</term><term>Gene Frequency</term><term>Genetic Linkage</term><term>Genetic Markers (genetics)</term><term>Heterozygote</term><term>Polymorphism, Single Nucleotide</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Cartographie de contigs</term><term>Cynara (génétique)</term><term>DNA restriction enzymes (métabolisme)</term><term>Fréquence d'allèle</term><term>Hétérozygote</term><term>Liaison génétique</term><term>Marqueurs génétiques (génétique)</term><term>Polymorphisme de nucléotide simple</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Genetic Markers</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA Restriction Enzymes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cynara</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cynara</term><term>Marqueurs génétiques</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>DNA restriction enzymes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Contig Mapping</term><term>Gene Frequency</term><term>Genetic Linkage</term><term>Heterozygote</term><term>Polymorphism, Single Nucleotide</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Cartographie de contigs</term><term>Fréquence d'allèle</term><term>Hétérozygote</term><term>Liaison génétique</term><term>Polymorphisme de nucléotide simple</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The globe artichoke (Cynara cardunculus L. var. scolymus) genome is relatively poorly explored, especially compared to those of the other major Asteraceae crops sunflower and lettuce. No SNP markers are in the public domain. We have combined the recently developed restriction-site associated DNA (RAD) approach with the Illumina DNA sequencing platform to effect the rapid and mass discovery of SNP markers for C. cardunculus.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22214349</PMID><DateCompleted><Year>2012</Year><Month>05</Month><Day>23</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2164</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><PubDate><Year>2012</Year><Month>Jan</Month><Day>03</Day></PubDate></JournalIssue><Title>BMC genomics</Title><ISOAbbreviation>BMC Genomics</ISOAbbreviation></Journal><ArticleTitle>RAD tag sequencing as a source of SNP markers in Cynara cardunculus L.</ArticleTitle><Pagination><MedlinePgn>3</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/1471-2164-13-3</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The globe artichoke (Cynara cardunculus L. var. scolymus) genome is relatively poorly explored, especially compared to those of the other major Asteraceae crops sunflower and lettuce. No SNP markers are in the public domain. We have combined the recently developed restriction-site associated DNA (RAD) approach with the Illumina DNA sequencing platform to effect the rapid and mass discovery of SNP markers for C. cardunculus.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">RAD tags were sequenced from the genomic DNA of three C. cardunculus mapping population parents, generating 9.7 million reads, corresponding to ~1 Gbp of sequence. An assembly based on paired ends produced ~6.0 Mbp of genomic sequence, separated into ~19,000 contigs (mean length 312 bp), of which ~21% were fragments of putative coding sequence. The shared sequences allowed for the discovery of ~34,000 SNPs and nearly 800 indels, equivalent to a SNP frequency of 5.6 per 1,000 nt, and an indel frequency of 0.2 per 1,000 nt. A sample of heterozygous SNP loci was mapped by CAPS assays and this exercise provided validation of our mining criteria. The repetitive fraction of the genome had a high representation of retrotransposon sequence, followed by simple repeats, AT-low complexity regions and mobile DNA elements. The genomic k-mers distribution and CpG rate of C. cardunculus, compared with data derived from three whole genome-sequenced dicots species, provided a further evidence of the random representation of the C. cardunculus genome generated by RAD sampling.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The RAD tag sequencing approach is a cost-effective and rapid method to develop SNP markers in a highly heterozygous species. Our approach permitted to generate a large and robust SNP datasets by the adoption of optimized filtering criteria.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Scaglione</LastName><ForeName>Davide</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Di.Va.P.R.A. Plant Genetics and Breeding, University of Torino, via L. da Vinci44, 10095 Grugliasco (Torino), Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Acquadro</LastName><ForeName>Alberto</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Portis</LastName><ForeName>Ezio</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Tirone</LastName><ForeName>Matteo</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Knapp</LastName><ForeName>Steven J</ForeName><Initials>SJ</Initials></Author><Author ValidYN="Y"><LastName>Lanteri</LastName><ForeName>Sergio</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>01</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Genomics</MedlineTA><NlmUniqueID>100965258</NlmUniqueID><ISSNLinking>1471-2164</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005819">Genetic Markers</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.21.-</RegistryNumber><NameOfSubstance UI="D004262">DNA Restriction Enzymes</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020451" MajorTopicYN="N">Contig Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D031942" MajorTopicYN="N">Cynara</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004262" MajorTopicYN="N">DNA Restriction Enzymes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005787" MajorTopicYN="N">Gene Frequency</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008040" MajorTopicYN="N">Genetic Linkage</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005819" MajorTopicYN="N">Genetic Markers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006579" MajorTopicYN="N">Heterozygote</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020641" MajorTopicYN="Y">Polymorphism, Single Nucleotide</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="Y">Sequence Analysis, DNA</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>08</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>01</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>5</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22214349</ArticleId><ArticleId IdType="pii">1471-2164-13-3</ArticleId><ArticleId IdType="doi">10.1186/1471-2164-13-3</ArticleId><ArticleId IdType="pmc">PMC3269995</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Genome Res. 2007 Feb;17(2):240-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17189378</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2007 Sep 27;449(7161):463-7</Citation><ArticleIdList><ArticleId IdType="pubmed">17721507</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2007;2(12):e1326</Citation><ArticleIdList><ArticleId IdType="pubmed">18094749</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2008 May;18(5):821-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18349386</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Bioinformatics. 2008;9:253</Citation><ArticleIdList><ArticleId IdType="pubmed">18510760</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2008;3(10):e3376</Citation><ArticleIdList><ArticleId IdType="pubmed">18852878</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2008 Nov;18(11):1851-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18714091</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2009 Feb 6;136(3):461-72</Citation><ArticleIdList><ArticleId IdType="pubmed">19203581</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Plant Biol. 2009;9:30</Citation><ArticleIdList><ArticleId IdType="pubmed">19292932</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2009;60:43-66</Citation><ArticleIdList><ArticleId IdType="pubmed">19007329</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2009;10:454</Citation><ArticleIdList><ArticleId IdType="pubmed">19785740</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2009;10(10):R108</Citation><ArticleIdList><ArticleId IdType="pubmed">19814784</ArticleId></ArticleIdList></Reference><Reference><Citation>Theor Appl Genet. 2009 Dec;120(1):59-70</Citation><ArticleIdList><ArticleId IdType="pubmed">19787331</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2011;12:4</Citation><ArticleIdList><ArticleId IdType="pubmed">21205322</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 2011 Feb;43(2):109-16</Citation><ArticleIdList><ArticleId IdType="pubmed">21186353</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2011 Mar 15;27(6):764-70</Citation><ArticleIdList><ArticleId IdType="pubmed">21217122</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ecol Resour. 2011 Mar;11 Suppl 1:117-22</Citation><ArticleIdList><ArticleId IdType="pubmed">21429168</ArticleId></ArticleIdList></Reference><Reference><Citation>Theor Appl Genet. 2011 May;122(8):1467-80</Citation><ArticleIdList><ArticleId IdType="pubmed">21344184</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2011;6(4):e18561</Citation><ArticleIdList><ArticleId IdType="pubmed">21541009</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2011;6(4):e19315</Citation><ArticleIdList><ArticleId IdType="pubmed">21541297</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2011;12:304</Citation><ArticleIdList><ArticleId IdType="pubmed">21663628</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2011 Aug 15;27(16):2187-93</Citation><ArticleIdList><ArticleId IdType="pubmed">21712251</ArticleId></ArticleIdList></Reference><Reference><Citation>Theor Appl Genet. 2011 Nov;123(7):1215-29</Citation><ArticleIdList><ArticleId IdType="pubmed">21800142</ArticleId></ArticleIdList></Reference><Reference><Citation>J Hered. 2002 Jan-Feb;93(1):77-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12011185</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytother Res. 2002 Jun;16(4):368-72</Citation><ArticleIdList><ArticleId IdType="pubmed">12112295</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioorg Med Chem Lett. 2003 Jan 20;13(2):223-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12482428</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2003 Apr 1;170(7):3614-20</Citation><ArticleIdList><ArticleId IdType="pubmed">12646625</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 Dec 19;302(5653):2115-7</Citation><ArticleIdList><ArticleId IdType="pubmed">14684820</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2004;32(1):e5</Citation><ArticleIdList><ArticleId IdType="pubmed">14704362</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2004 Jun 30;52(13):4090-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15212452</ArticleId></ArticleIdList></Reference><Reference><Citation>Biol Cell. 1993;78(1-2):41-51</Citation><ArticleIdList><ArticleId IdType="pubmed">8220226</ArticleId></ArticleIdList></Reference><Reference><Citation>Toxicol Appl Pharmacol. 1997 Jun;144(2):279-86</Citation><ArticleIdList><ArticleId IdType="pubmed">9194411</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10227-32</Citation><ArticleIdList><ArticleId IdType="pubmed">9294192</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2005 Sep 15;21(18):3674-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16081474</ArticleId></ArticleIdList></Reference><Reference><Citation>Theor Appl Genet. 2006 May;112(8):1532-42</Citation><ArticleIdList><ArticleId IdType="pubmed">16565844</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Acquadro, Alberto" sort="Acquadro, Alberto" uniqKey="Acquadro A" first="Alberto" last="Acquadro">Alberto Acquadro</name><name sortKey="Knapp, Steven J" sort="Knapp, Steven J" uniqKey="Knapp S" first="Steven J" last="Knapp">Steven J. Knapp</name><name sortKey="Lanteri, Sergio" sort="Lanteri, Sergio" uniqKey="Lanteri S" first="Sergio" last="Lanteri">Sergio Lanteri</name><name sortKey="Portis, Ezio" sort="Portis, Ezio" uniqKey="Portis E" first="Ezio" last="Portis">Ezio Portis</name><name sortKey="Tirone, Matteo" sort="Tirone, Matteo" uniqKey="Tirone M" first="Matteo" last="Tirone">Matteo Tirone</name></noCountry><country name="Italie"><noRegion><name sortKey="Scaglione, Davide" sort="Scaglione, Davide" uniqKey="Scaglione D" first="Davide" last="Scaglione">Davide Scaglione</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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