MersV1, PubMed, Checkpoint, bibRecord, 001890

Host species restriction of Middle East respiratory syndrome coronavirus through its receptor, dipeptidyl peptidase 4.

Identifieur interne : 001890 ( PubMed/Checkpoint ); précédent : 001889; suivant : 001891

Host species restriction of Middle East respiratory syndrome coronavirus through its receptor, dipeptidyl peptidase 4.

Auteurs : Neeltje Van Doremalen [États-Unis] ; Kerri L. Miazgowicz [États-Unis] ; Shauna Milne-Price [États-Unis] ; Trenton Bushmaker [États-Unis] ; Shelly Robertson [États-Unis] ; Dana Scott [États-Unis] ; Joerg Kinne [Émirats arabes unis] ; Jason S. Mclellan [États-Unis] ; Jiang Zhu [États-Unis] ; Vincent J. Munster

Source :

Journal of virology [ 1098-5514 ] ; 2014.

RBID : pubmed:24899185

Descripteurs français

KwdFr :
- Animaux, Bovins, Bétail (métabolisme), Bétail (virologie), Capra (métabolisme), Capra (virologie), Cellules Vero, Chameaux (métabolisme), Chameaux (virologie), Coronavirus (métabolisme), Coronavirus (pathogénicité), Cricetinae, Dipeptidyl peptidase 4 (métabolisme), Furets (métabolisme), Furets (virologie), Humains, Liaison aux protéines, Lignée cellulaire, Lignée cellulaire tumorale, Macaca mulatta (métabolisme), Macaca mulatta (virologie), Moyen Orient, Ovis (métabolisme), Ovis (virologie), Primates (métabolisme), Primates (virologie), Récepteurs viraux (métabolisme), Réplication virale (génétique), Souris, Souris de lignée C57BL, Spécificité d'hôte, Tropisme viral, Virus respiratoires syncytiaux (métabolisme), Virus respiratoires syncytiaux (pathogénicité).
MESH :
- génétique : Réplication virale.
- métabolisme : Bétail, Capra, Chameaux, Coronavirus, Dipeptidyl peptidase 4, Furets, Macaca mulatta, Ovis, Primates, Récepteurs viraux, Virus respiratoires syncytiaux.
- pathogénicité : Coronavirus, Virus respiratoires syncytiaux.
- virologie : Bétail, Capra, Chameaux, Furets, Macaca mulatta, Ovis, Primates.
- Animaux, Bovins, Cellules Vero, Cricetinae, Humains, Liaison aux protéines, Lignée cellulaire, Lignée cellulaire tumorale, Moyen Orient, Souris, Souris de lignée C57BL, Spécificité d'hôte, Tropisme viral.

English descriptors

KwdEn :
- Animals, Camelus (metabolism), Camelus (virology), Cattle, Cell Line, Cell Line, Tumor, Coronavirus (metabolism), Coronavirus (pathogenicity), Cricetinae, Dipeptidyl Peptidase 4 (metabolism), Ferrets (metabolism), Ferrets (virology), Goats (metabolism), Goats (virology), Host Specificity, Humans, Livestock (metabolism), Livestock (virology), Macaca mulatta (metabolism), Macaca mulatta (virology), Mice, Mice, Inbred C57BL, Middle East, Primates (metabolism), Primates (virology), Protein Binding, Receptors, Virus (metabolism), Respiratory Syncytial Viruses (metabolism), Respiratory Syncytial Viruses (pathogenicity), Sheep (metabolism), Sheep (virology), Vero Cells, Viral Tropism, Virus Replication (genetics).
MESH :
- chemical , metabolism : Dipeptidyl Peptidase 4, Receptors, Virus.
- geographic : Middle East.
- genetics : Virus Replication.
- metabolism : Camelus, Coronavirus, Ferrets, Goats, Livestock, Macaca mulatta, Primates, Respiratory Syncytial Viruses, Sheep.
- pathogenicity : Coronavirus, Respiratory Syncytial Viruses.
- virology : Camelus, Ferrets, Goats, Livestock, Macaca mulatta, Primates, Sheep.
- Animals, Cattle, Cell Line, Cell Line, Tumor, Cricetinae, Host Specificity, Humans, Mice, Mice, Inbred C57BL, Protein Binding, Vero Cells, Viral Tropism.

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in 2012. Recently, the MERS-CoV receptor dipeptidyl peptidase 4 (DPP4) was identified and the specific interaction of the receptor-binding domain (RBD) of MERS-CoV spike protein and DPP4 was determined by crystallography. Animal studies identified rhesus macaques but not hamsters, ferrets, or mice to be susceptible for MERS-CoV. Here, we investigated the role of DPP4 in this observed species tropism. Cell lines of human and nonhuman primate origin were permissive of MERS-CoV, whereas hamster, ferret, or mouse cell lines were not, despite the presence of DPP4. Expression of human DPP4 in nonsusceptible BHK and ferret cells enabled MERS-CoV replication, whereas expression of hamster or ferret DPP4 did not. Modeling the binding energies of MERS-CoV spike protein RBD to DPP4 of human (susceptible) or hamster (nonsusceptible) identified five amino acid residues involved in the DPP4-RBD interaction. Expression of hamster DPP4 containing the five human DPP4 amino acids rendered BHK cells susceptible to MERS-CoV, whereas expression of human DPP4 containing the five hamster DPP4 amino acids did not. Using the same approach, the potential of MERS-CoV to utilize the DPP4s of common Middle Eastern livestock was investigated. Modeling of the DPP4 and MERS-CoV RBD interaction predicted the ability of MERS-CoV to bind the DPP4s of camel, goat, cow, and sheep. Expression of the DPP4s of these species on BHK cells supported MERS-CoV replication. This suggests, together with the abundant DPP4 presence in the respiratory tract, that these species might be able to function as a MERS-CoV intermediate reservoir.

DOI: 10.1128/JVI.00676-14
PubMed: 24899185

Affiliations:

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Le document en format XML

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<author><name sortKey="Zhu, Jiang" sort="Zhu, Jiang" uniqKey="Zhu J" first="Jiang" last="Zhu">Jiang Zhu</name>
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<country xml:lang="fr">États-Unis</country>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Camelus (metabolism)</term>
<term>Camelus (virology)</term>
<term>Cattle</term>
<term>Cell Line</term>
<term>Cell Line, Tumor</term>
<term>Coronavirus (metabolism)</term>
<term>Coronavirus (pathogenicity)</term>
<term>Cricetinae</term>
<term>Dipeptidyl Peptidase 4 (metabolism)</term>
<term>Ferrets (metabolism)</term>
<term>Ferrets (virology)</term>
<term>Goats (metabolism)</term>
<term>Goats (virology)</term>
<term>Host Specificity</term>
<term>Humans</term>
<term>Livestock (metabolism)</term>
<term>Livestock (virology)</term>
<term>Macaca mulatta (metabolism)</term>
<term>Macaca mulatta (virology)</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Middle East</term>
<term>Primates (metabolism)</term>
<term>Primates (virology)</term>
<term>Protein Binding</term>
<term>Receptors, Virus (metabolism)</term>
<term>Respiratory Syncytial Viruses (metabolism)</term>
<term>Respiratory Syncytial Viruses (pathogenicity)</term>
<term>Sheep (metabolism)</term>
<term>Sheep (virology)</term>
<term>Vero Cells</term>
<term>Viral Tropism</term>
<term>Virus Replication (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Bovins</term>
<term>Bétail (métabolisme)</term>
<term>Bétail (virologie)</term>
<term>Capra (métabolisme)</term>
<term>Capra (virologie)</term>
<term>Cellules Vero</term>
<term>Chameaux (métabolisme)</term>
<term>Chameaux (virologie)</term>
<term>Coronavirus (métabolisme)</term>
<term>Coronavirus (pathogénicité)</term>
<term>Cricetinae</term>
<term>Dipeptidyl peptidase 4 (métabolisme)</term>
<term>Furets (métabolisme)</term>
<term>Furets (virologie)</term>
<term>Humains</term>
<term>Liaison aux protéines</term>
<term>Lignée cellulaire</term>
<term>Lignée cellulaire tumorale</term>
<term>Macaca mulatta (métabolisme)</term>
<term>Macaca mulatta (virologie)</term>
<term>Moyen Orient</term>
<term>Ovis (métabolisme)</term>
<term>Ovis (virologie)</term>
<term>Primates (métabolisme)</term>
<term>Primates (virologie)</term>
<term>Récepteurs viraux (métabolisme)</term>
<term>Réplication virale (génétique)</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Spécificité d'hôte</term>
<term>Tropisme viral</term>
<term>Virus respiratoires syncytiaux (métabolisme)</term>
<term>Virus respiratoires syncytiaux (pathogénicité)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Dipeptidyl Peptidase 4</term>
<term>Receptors, Virus</term>
</keywords>
<keywords scheme="MESH" type="geographic" xml:lang="en"><term>Middle East</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Réplication virale</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Camelus</term>
<term>Coronavirus</term>
<term>Ferrets</term>
<term>Goats</term>
<term>Livestock</term>
<term>Macaca mulatta</term>
<term>Primates</term>
<term>Respiratory Syncytial Viruses</term>
<term>Sheep</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bétail</term>
<term>Capra</term>
<term>Chameaux</term>
<term>Coronavirus</term>
<term>Dipeptidyl peptidase 4</term>
<term>Furets</term>
<term>Macaca mulatta</term>
<term>Ovis</term>
<term>Primates</term>
<term>Récepteurs viraux</term>
<term>Virus respiratoires syncytiaux</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Coronavirus</term>
<term>Respiratory Syncytial Viruses</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus</term>
<term>Virus respiratoires syncytiaux</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Bétail</term>
<term>Capra</term>
<term>Chameaux</term>
<term>Furets</term>
<term>Macaca mulatta</term>
<term>Ovis</term>
<term>Primates</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Camelus</term>
<term>Ferrets</term>
<term>Goats</term>
<term>Livestock</term>
<term>Macaca mulatta</term>
<term>Primates</term>
<term>Sheep</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cattle</term>
<term>Cell Line</term>
<term>Cell Line, Tumor</term>
<term>Cricetinae</term>
<term>Host Specificity</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Protein Binding</term>
<term>Vero Cells</term>
<term>Viral Tropism</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Bovins</term>
<term>Cellules Vero</term>
<term>Cricetinae</term>
<term>Humains</term>
<term>Liaison aux protéines</term>
<term>Lignée cellulaire</term>
<term>Lignée cellulaire tumorale</term>
<term>Moyen Orient</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Spécificité d'hôte</term>
<term>Tropisme viral</term>
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<front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in 2012. Recently, the MERS-CoV receptor dipeptidyl peptidase 4 (DPP4) was identified and the specific interaction of the receptor-binding domain (RBD) of MERS-CoV spike protein and DPP4 was determined by crystallography. Animal studies identified rhesus macaques but not hamsters, ferrets, or mice to be susceptible for MERS-CoV. Here, we investigated the role of DPP4 in this observed species tropism. Cell lines of human and nonhuman primate origin were permissive of MERS-CoV, whereas hamster, ferret, or mouse cell lines were not, despite the presence of DPP4. Expression of human DPP4 in nonsusceptible BHK and ferret cells enabled MERS-CoV replication, whereas expression of hamster or ferret DPP4 did not. Modeling the binding energies of MERS-CoV spike protein RBD to DPP4 of human (susceptible) or hamster (nonsusceptible) identified five amino acid residues involved in the DPP4-RBD interaction. Expression of hamster DPP4 containing the five human DPP4 amino acids rendered BHK cells susceptible to MERS-CoV, whereas expression of human DPP4 containing the five hamster DPP4 amino acids did not. Using the same approach, the potential of MERS-CoV to utilize the DPP4s of common Middle Eastern livestock was investigated. Modeling of the DPP4 and MERS-CoV RBD interaction predicted the ability of MERS-CoV to bind the DPP4s of camel, goat, cow, and sheep. Expression of the DPP4s of these species on BHK cells supported MERS-CoV replication. This suggests, together with the abundant DPP4 presence in the respiratory tract, that these species might be able to function as a MERS-CoV intermediate reservoir.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24899185</PMID>
<DateCompleted><Year>2014</Year>
<Month>09</Month>
<Day>25</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>03</Month>
<Day>20</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-5514</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>88</Volume>
<Issue>16</Issue>
<PubDate><Year>2014</Year>
<Month>Aug</Month>
</PubDate>
</JournalIssue>
<Title>Journal of virology</Title>
<ISOAbbreviation>J. Virol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Host species restriction of Middle East respiratory syndrome coronavirus through its receptor, dipeptidyl peptidase 4.</ArticleTitle>
<Pagination><MedlinePgn>9220-32</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1128/JVI.00676-14</ELocationID>
<Abstract><AbstractText Label="UNLABELLED">Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in 2012. Recently, the MERS-CoV receptor dipeptidyl peptidase 4 (DPP4) was identified and the specific interaction of the receptor-binding domain (RBD) of MERS-CoV spike protein and DPP4 was determined by crystallography. Animal studies identified rhesus macaques but not hamsters, ferrets, or mice to be susceptible for MERS-CoV. Here, we investigated the role of DPP4 in this observed species tropism. Cell lines of human and nonhuman primate origin were permissive of MERS-CoV, whereas hamster, ferret, or mouse cell lines were not, despite the presence of DPP4. Expression of human DPP4 in nonsusceptible BHK and ferret cells enabled MERS-CoV replication, whereas expression of hamster or ferret DPP4 did not. Modeling the binding energies of MERS-CoV spike protein RBD to DPP4 of human (susceptible) or hamster (nonsusceptible) identified five amino acid residues involved in the DPP4-RBD interaction. Expression of hamster DPP4 containing the five human DPP4 amino acids rendered BHK cells susceptible to MERS-CoV, whereas expression of human DPP4 containing the five hamster DPP4 amino acids did not. Using the same approach, the potential of MERS-CoV to utilize the DPP4s of common Middle Eastern livestock was investigated. Modeling of the DPP4 and MERS-CoV RBD interaction predicted the ability of MERS-CoV to bind the DPP4s of camel, goat, cow, and sheep. Expression of the DPP4s of these species on BHK cells supported MERS-CoV replication. This suggests, together with the abundant DPP4 presence in the respiratory tract, that these species might be able to function as a MERS-CoV intermediate reservoir.</AbstractText>
<AbstractText Label="IMPORTANCE" NlmCategory="OBJECTIVE">The ongoing outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) has caused 701 laboratory-confirmed cases to date, with 249 fatalities. Although bats and dromedary camels have been identified as potential MERS-CoV hosts, the virus has so far not been isolated from any species other than humans. The inability of MERS-CoV to infect commonly used animal models, such as hamster, mice, and ferrets, indicates the presence of a species barrier. We show that the MERS-CoV receptor DPP4 plays a pivotal role in the observed species tropism of MERS-CoV and subsequently identified the amino acids in DPP4 responsible for this restriction. Using a combined modeling and experimental approach, we predict that, based on the ability of MERS-CoV to utilize the DPP4 of common Middle East livestock species, such as camels, goats, sheep, and cows, these form a potential MERS-CoV intermediate host reservoir species.</AbstractText>
<CopyrightInformation>Copyright © 2014, American Society for Microbiology. All Rights Reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>van Doremalen</LastName>
<ForeName>Neeltje</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Miazgowicz</LastName>
<ForeName>Kerri L</ForeName>
<Initials>KL</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Milne-Price</LastName>
<ForeName>Shauna</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bushmaker</LastName>
<ForeName>Trenton</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Robertson</LastName>
<ForeName>Shelly</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Scott</LastName>
<ForeName>Dana</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kinne</LastName>
<ForeName>Joerg</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Central Veterinary Research Laboratories, Dubai, Dubai, United Arab Emirates.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>McLellan</LastName>
<ForeName>Jason S</ForeName>
<Initials>JS</Initials>
<AffiliationInfo><Affiliation>Department of Biochemistry, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhu</LastName>
<ForeName>Jiang</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Department of Immunology and Microbial Science and Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Munster</LastName>
<ForeName>Vincent J</ForeName>
<Initials>VJ</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA vincent.munster@nih.gov.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><Agency>Intramural NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D052060">Research Support, N.I.H., Intramural</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2014</Year>
<Month>06</Month>
<Day>04</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>J Virol</MedlineTA>
<NlmUniqueID>0113724</NlmUniqueID>
<ISSNLinking>0022-538X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011991">Receptors, Virus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C078034">coronavirus receptors</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.14.5</RegistryNumber>
<NameOfSubstance UI="D018819">Dipeptidyl Peptidase 4</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002162" MajorTopicYN="N">Camelus</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002417" MajorTopicYN="N">Cattle</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045744" MajorTopicYN="N">Cell Line, Tumor</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017934" MajorTopicYN="N">Coronavirus</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006224" MajorTopicYN="N">Cricetinae</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018819" MajorTopicYN="N">Dipeptidyl Peptidase 4</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005289" MajorTopicYN="N">Ferrets</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006041" MajorTopicYN="N">Goats</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D058507" MajorTopicYN="Y">Host Specificity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D058751" MajorTopicYN="N">Livestock</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008253" MajorTopicYN="N">Macaca mulatta</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008810" MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008877" MajorTopicYN="N" Type="Geographic">Middle East</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011323" MajorTopicYN="N">Primates</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011991" MajorTopicYN="N">Receptors, Virus</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012136" MajorTopicYN="N">Respiratory Syncytial Viruses</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012756" MajorTopicYN="N">Sheep</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D056189" MajorTopicYN="N">Viral Tropism</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
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</ArticleIdList>
<ReferenceList><Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2014;5(3):e01146-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24781747</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Crit Rev Clin Lab Sci. 2003 Jun;40(3):209-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12892317</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteins. 2003;53 Suppl 6:430-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14579332</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2007 Nov 1;23(21):2947-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17846036</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2012;3(6). pii: e00515-12. doi: 10.1128/mBio.00515-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23232719</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2013;4(1):e00611-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23422412</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2013 Mar 14;495(7440):251-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23486063</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2013 Apr 5;340(6128):17-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23559228</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2013 Apr 18;368(16):1560-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23550601</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Rep. 2013;3:1686</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23594967</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2013 Mar;19(3):456-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23622767</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2013 Jun 1;207(11):1743-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23532101</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2013 Jun 1;207(11):1630-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23532103</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Jun;87(12):6604-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23552422</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2013 Jun 29;381(9885):2265-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23727167</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013;8(7):e69127</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23844250</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Aug;87(16):9379-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23785207</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Res. 2013 Aug;23(8):986-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23835475</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2013 Aug 8;500(7461):227-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23831647</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet Infect Dis. 2013 Sep;13(9):745-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23782859</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2013;4(5):e00650-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24023385</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2013 Oct;19(10):1697-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24050621</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet Infect Dis. 2013 Oct;13(10):859-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23933067</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2013;18(36):pii=20574</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24079378</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16157-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24043791</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Nov;87(21):11950-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23986586</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2013 Oct;19(10):1313-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24013700</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16598-603</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24062443</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2013 Nov;19(11):1819-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24206838</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2013 Dec 14;382(9909):1993-2002</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24055451</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2013;18(50):20662</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24342516</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2014 Feb;88(3):1834-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24257613</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet Infect Dis. 2014 Feb;14(2):140-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24355866</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2014;5(1). pii: e01062-13. doi: 10.1128/mBio.01062-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24549846</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2014;5(2):e00884-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24570370</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2014 May;88(9):4953-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24554656</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2014 May;88(9):5195-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24574399</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):4970-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24599590</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Protein Sci. 2002 Nov;11(11):2714-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12381853</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Émirats arabes unis</li>
<li>États-Unis</li>
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<li>Montana</li>
<li>New Hampshire</li>
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<tree><noCountry><name sortKey="Munster, Vincent J" sort="Munster, Vincent J" uniqKey="Munster V" first="Vincent J" last="Munster">Vincent J. Munster</name>
</noCountry>
<country name="États-Unis"><region name="Montana"><name sortKey="Van Doremalen, Neeltje" sort="Van Doremalen, Neeltje" uniqKey="Van Doremalen N" first="Neeltje" last="Van Doremalen">Neeltje Van Doremalen</name>
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<name sortKey="Bushmaker, Trenton" sort="Bushmaker, Trenton" uniqKey="Bushmaker T" first="Trenton" last="Bushmaker">Trenton Bushmaker</name>
<name sortKey="Mclellan, Jason S" sort="Mclellan, Jason S" uniqKey="Mclellan J" first="Jason S" last="Mclellan">Jason S. Mclellan</name>
<name sortKey="Miazgowicz, Kerri L" sort="Miazgowicz, Kerri L" uniqKey="Miazgowicz K" first="Kerri L" last="Miazgowicz">Kerri L. Miazgowicz</name>
<name sortKey="Milne Price, Shauna" sort="Milne Price, Shauna" uniqKey="Milne Price S" first="Shauna" last="Milne-Price">Shauna Milne-Price</name>
<name sortKey="Robertson, Shelly" sort="Robertson, Shelly" uniqKey="Robertson S" first="Shelly" last="Robertson">Shelly Robertson</name>
<name sortKey="Scott, Dana" sort="Scott, Dana" uniqKey="Scott D" first="Dana" last="Scott">Dana Scott</name>
<name sortKey="Zhu, Jiang" sort="Zhu, Jiang" uniqKey="Zhu J" first="Jiang" last="Zhu">Jiang Zhu</name>
</country>
<country name="Émirats arabes unis"><noRegion><name sortKey="Kinne, Joerg" sort="Kinne, Joerg" uniqKey="Kinne J" first="Joerg" last="Kinne">Joerg Kinne</name>
</noRegion>
</country>
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HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i   -Sk "pubmed:24899185" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

Host species restriction of Middle East respiratory syndrome coronavirus through its receptor, dipeptidyl peptidase 4.

Host species restriction of Middle East respiratory syndrome coronavirus through its receptor, dipeptidyl peptidase 4.

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