Synthesis of capped RNA using a DMT group as a purification handle.
Identifieur interne : 001726 ( PubMed/Checkpoint ); précédent : 001725; suivant : 001727Synthesis of capped RNA using a DMT group as a purification handle.
Auteurs : Elizabeth Veliath [États-Unis] ; Barbara L. Gaffney ; Roger A. JonesSource :
- Nucleosides, nucleotides & nucleic acids [ 1532-2335 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- génétique : ARN messager.
- isolement et purification : ARN.
- ARN, Coiffes des ARN, Oligonucléotides, Stabilité de l'ARN.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Oligonucleotides, RNA, RNA Caps.
- chemical , genetics : RNA, Messenger.
- chemical , isolation & purification : RNA.
- RNA Stability.
Abstract
We report a new method for synthesis of capped RNA or 2'-OMe RNA that uses a N(2-)4,4'-dimethoxytrityl (DMT) group as a lipophilic purification handle to allow convenient isolation and purification of the capped RNA. The DMT group is easily removed under mild conditions without degradation of the cap. We have used this approach to prepare capped 10- and 20-mers. This method is compatible with the many condensation reactions that have been reported for preparation of capped RNA or cap analogues.
DOI: 10.1080/15257770.2013.864417
PubMed: 24588755
Affiliations:
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Gaffney</name><name sortKey="Jones, Roger A" sort="Jones, Roger A" uniqKey="Jones R" first="Roger A" last="Jones">Roger A. Jones</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Veliath, Elizabeth" sort="Veliath, Elizabeth" uniqKey="Veliath E" first="Elizabeth" last="Veliath">Elizabeth Veliath</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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