Virological and serological analysis of a recent Middle East respiratory syndrome coronavirus infection case on a triple combination antiviral regimen.
Identifieur interne : 001691 ( PubMed/Checkpoint ); précédent : 001690; suivant : 001692Virological and serological analysis of a recent Middle East respiratory syndrome coronavirus infection case on a triple combination antiviral regimen.
Auteurs : Nikolaos Spanakis [Grèce] ; Sotirios Tsiodras [Grèce] ; Bart L. Haagmans [Pays-Bas] ; V Stalin Raj [Pays-Bas] ; Kostantinos Pontikis [Grèce] ; Antonia Koutsoukou [Grèce] ; Nikolaos G. Koulouris [Grèce] ; Albert D M E. Osterhaus [Pays-Bas] ; Marion P G. Koopmans [Pays-Bas] ; Athanassios Tsakris [Grèce]Source :
- International journal of antimicrobial agents [ 1872-7913 ] ; 2014.
Descripteurs français
- KwdFr :
- Antiviraux (usage thérapeutique), Association de médicaments, Association médicamenteuse, Coronavirus du syndrome respiratoire du Moyen-Orient (), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Excrétion virale, Grèce, Humains, Infections de l'appareil respiratoire (traitement médicamenteux), Infections de l'appareil respiratoire (virologie), Infections à coronavirus (traitement médicamenteux), Infections à coronavirus (virologie), Lopinavir, Mâle, Phylogénie, Ribavirine (usage thérapeutique), Ritonavir, Virémie.
- MESH :
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient.
- immunologie : Coronavirus du syndrome respiratoire du Moyen-Orient.
- traitement médicamenteux : Infections de l'appareil respiratoire, Infections à coronavirus.
- usage thérapeutique : Antiviraux, Ribavirine.
- virologie : Infections de l'appareil respiratoire, Infections à coronavirus.
- Association de médicaments, Association médicamenteuse, Coronavirus du syndrome respiratoire du Moyen-Orient, Excrétion virale, Grèce, Humains, Lopinavir, Mâle, Phylogénie, Ritonavir, Virémie.
English descriptors
- KwdEn :
- Antiviral Agents (therapeutic use), Coronavirus Infections (drug therapy), Coronavirus Infections (virology), Drug Combinations, Drug Therapy, Combination, Greece, Humans, Lopinavir, Male, Middle East Respiratory Syndrome Coronavirus (drug effects), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Phylogeny, Respiratory Tract Infections (drug therapy), Respiratory Tract Infections (virology), Ribavirin (therapeutic use), Ritonavir, Viremia, Virus Shedding.
- MESH :
- chemical , therapeutic use : Antiviral Agents, Ribavirin.
- drug effects : Middle East Respiratory Syndrome Coronavirus.
- drug therapy : Coronavirus Infections, Respiratory Tract Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- virology : Coronavirus Infections, Respiratory Tract Infections.
- chemical : Drug Combinations, Drug Therapy, Combination, Greece, Humans, Lopinavir, Male, Phylogeny, Ritonavir, Viremia, Virus Shedding.
Abstract
Serological, molecular and phylogenetic analyses of a recently imported case of Middle East respiratory syndrome coronavirus (MERS-CoV) in Greece are reported. Although MERS-CoV remained detectable in the respiratory tract secretions of the patient until the fourth week of illness, viraemia was last detected 2 days after initiation of triple combination therapy with pegylated interferon, ribavirin and lopinavir/ritonavir, administered from Day 13 of illness. Phylogenetic analysis of the virus showed close similarity with other human MERS-CoVs from the recent Jeddah outbreak in Saudi Arabia. Immunoglobulin G (IgG) titres peaked 3 weeks after the onset of illness, whilst IgM levels remained constantly elevated during the follow-up period (second to fifth week of illness). Serological testing confirmed by virus neutralisation assay detected an additional case that was a close contact of the patient.
DOI: 10.1016/j.ijantimicag.2014.07.026
PubMed: 25288266
Affiliations:
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Koulouris</name><affiliation wicri:level="3"><nlm:affiliation>First Department of Respiratory Medicine, University of Athens Medical School and 'Sotiria' Chest Diseases Hospital, Athens, Greece.</nlm:affiliation><country xml:lang="fr">Grèce</country><wicri:regionArea>First Department of Respiratory Medicine, University of Athens Medical School and 'Sotiria' Chest Diseases Hospital, Athens</wicri:regionArea><placeName><settlement type="city">Athènes</settlement><region nuts="2" type="region">Attique (région)</region></placeName></affiliation></author><author><name sortKey="Osterhaus, Albert D M E" sort="Osterhaus, Albert D M E" uniqKey="Osterhaus A" first="Albert D M E" last="Osterhaus">Albert D M E. Osterhaus</name><affiliation wicri:level="3"><nlm:affiliation>Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus Medical Center, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Koopmans, Marion P G" sort="Koopmans, Marion P G" uniqKey="Koopmans M" first="Marion P G" last="Koopmans">Marion P G. Koopmans</name><affiliation wicri:level="3"><nlm:affiliation>Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus Medical Center, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Tsakris, Athanassios" sort="Tsakris, Athanassios" uniqKey="Tsakris A" first="Athanassios" last="Tsakris">Athanassios Tsakris</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Athens, Medical School, 75 Mikras Asias Street, 115 27 Athens, Greece. 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Although MERS-CoV remained detectable in the respiratory tract secretions of the patient until the fourth week of illness, viraemia was last detected 2 days after initiation of triple combination therapy with pegylated interferon, ribavirin and lopinavir/ritonavir, administered from Day 13 of illness. Phylogenetic analysis of the virus showed close similarity with other human MERS-CoVs from the recent Jeddah outbreak in Saudi Arabia. Immunoglobulin G (IgG) titres peaked 3 weeks after the onset of illness, whilst IgM levels remained constantly elevated during the follow-up period (second to fifth week of illness). Serological testing confirmed by virus neutralisation assay detected an additional case that was a close contact of the patient. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25288266</PMID><DateCompleted><Year>2017</Year><Month>04</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-7913</ISSN><JournalIssue CitedMedium="Internet"><Volume>44</Volume><Issue>6</Issue><PubDate><Year>2014</Year><Month>Dec</Month></PubDate></JournalIssue><Title>International journal of antimicrobial agents</Title><ISOAbbreviation>Int. J. Antimicrob. Agents</ISOAbbreviation></Journal><ArticleTitle>Virological and serological analysis of a recent Middle East respiratory syndrome coronavirus infection case on a triple combination antiviral regimen.</ArticleTitle><Pagination><MedlinePgn>528-32</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ijantimicag.2014.07.026</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0924-8579(14)00278-7</ELocationID><Abstract><AbstractText>Serological, molecular and phylogenetic analyses of a recently imported case of Middle East respiratory syndrome coronavirus (MERS-CoV) in Greece are reported. Although MERS-CoV remained detectable in the respiratory tract secretions of the patient until the fourth week of illness, viraemia was last detected 2 days after initiation of triple combination therapy with pegylated interferon, ribavirin and lopinavir/ritonavir, administered from Day 13 of illness. Phylogenetic analysis of the virus showed close similarity with other human MERS-CoVs from the recent Jeddah outbreak in Saudi Arabia. Immunoglobulin G (IgG) titres peaked 3 weeks after the onset of illness, whilst IgM levels remained constantly elevated during the follow-up period (second to fifth week of illness). Serological testing confirmed by virus neutralisation assay detected an additional case that was a close contact of the patient. </AbstractText><CopyrightInformation>Copyright © 2014. Published by Elsevier B.V.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Spanakis</LastName><ForeName>Nikolaos</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Microbiology, University of Athens, Medical School, 75 Mikras Asias Street, 115 27 Athens, Greece.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tsiodras</LastName><ForeName>Sotirios</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Hellenic Centre for Disease Control and Prevention, Athens, Greece; Department of Internal Medicine, University of Athens Medical School, Athens, Greece.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Haagmans</LastName><ForeName>Bart L</ForeName><Initials>BL</Initials><AffiliationInfo><Affiliation>Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Raj</LastName><ForeName>V Stalin</ForeName><Initials>VS</Initials><AffiliationInfo><Affiliation>Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pontikis</LastName><ForeName>Kostantinos</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>First Department of Respiratory Medicine, University of Athens Medical School and 'Sotiria' Chest Diseases Hospital, Athens, Greece.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Koutsoukou</LastName><ForeName>Antonia</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>First Department of Respiratory Medicine, University of Athens Medical School and 'Sotiria' Chest Diseases Hospital, Athens, Greece.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Koulouris</LastName><ForeName>Nikolaos G</ForeName><Initials>NG</Initials><AffiliationInfo><Affiliation>First Department of Respiratory Medicine, University of Athens Medical School and 'Sotiria' Chest Diseases Hospital, Athens, Greece.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Osterhaus</LastName><ForeName>Albert D M E</ForeName><Initials>AD</Initials><AffiliationInfo><Affiliation>Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Koopmans</LastName><ForeName>Marion P G</ForeName><Initials>MP</Initials><AffiliationInfo><Affiliation>Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tsakris</LastName><ForeName>Athanassios</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Microbiology, University of Athens, Medical School, 75 Mikras Asias Street, 115 27 Athens, Greece. Electronic address: atsakris@med.uoa.gr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D002363">Case Reports</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>09</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Int J Antimicrob Agents</MedlineTA><NlmUniqueID>9111860</NlmUniqueID><ISSNLinking>0924-8579</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000998">Antiviral Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004338">Drug Combinations</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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