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ARK: Aggregation of Reads by K-Means for Estimation of Bacterial Community Composition.

Identifieur interne : 001652 ( PubMed/Checkpoint ); précédent : 001651; suivant : 001653

ARK: Aggregation of Reads by K-Means for Estimation of Bacterial Community Composition.

Auteurs : David Koslicki [États-Unis] ; Saikat Chatterjee [Suède] ; Damon Shahrivar [Suède] ; Alan W. Walker [Royaume-Uni] ; Suzanna C. Francis [Royaume-Uni] ; Louise J. Fraser [Royaume-Uni] ; Mikko Vehkaper [Royaume-Uni] ; Yueheng Lan [République populaire de Chine] ; Jukka Corander [Finlande]

Source :

RBID : pubmed:26496191

Descripteurs français

English descriptors

Abstract

Estimation of bacterial community composition from high-throughput sequenced 16S rRNA gene amplicons is a key task in microbial ecology. Since the sequence data from each sample typically consist of a large number of reads and are adversely impacted by different levels of biological and technical noise, accurate analysis of such large datasets is challenging.

DOI: 10.1371/journal.pone.0140644
PubMed: 26496191


Affiliations:


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pubmed:26496191

Le document en format XML

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<name sortKey="Corander, Jukka" sort="Corander, Jukka" uniqKey="Corander J" first="Jukka" last="Corander">Jukka Corander</name>
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<keywords scheme="KwdEn" xml:lang="en">
<term>Algorithms</term>
<term>Bacteria (classification)</term>
<term>Bacteria (genetics)</term>
<term>Cluster Analysis</term>
<term>DNA, Bacterial (chemistry)</term>
<term>DNA, Bacterial (genetics)</term>
<term>Feces (microbiology)</term>
<term>Humans</term>
<term>Internet</term>
<term>Metagenomics (methods)</term>
<term>Microbiota (genetics)</term>
<term>Polymerase Chain Reaction</term>
<term>RNA, Ribosomal, 16S (genetics)</term>
<term>Reproducibility of Results</term>
<term>Sequence Analysis, DNA</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr">
<term>ADN bactérien ()</term>
<term>ADN bactérien (génétique)</term>
<term>ARN ribosomique 16S (génétique)</term>
<term>Algorithmes</term>
<term>Analyse de regroupements</term>
<term>Analyse de séquence d'ADN</term>
<term>Bactéries ()</term>
<term>Bactéries (génétique)</term>
<term>Fèces (microbiologie)</term>
<term>Humains</term>
<term>Internet</term>
<term>Microbiote (génétique)</term>
<term>Métagénomique ()</term>
<term>Reproductibilité des résultats</term>
<term>Réaction de polymérisation en chaîne</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en">
<term>DNA, Bacterial</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en">
<term>Bacteria</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>Bacteria</term>
<term>DNA, Bacterial</term>
<term>Microbiota</term>
<term>RNA, Ribosomal, 16S</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr">
<term>ADN bactérien</term>
<term>ARN ribosomique 16S</term>
<term>Bactéries</term>
<term>Microbiote</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en">
<term>Metagenomics</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr">
<term>Fèces</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en">
<term>Feces</term>
</keywords>
<keywords scheme="MESH" xml:lang="en">
<term>Algorithms</term>
<term>Cluster Analysis</term>
<term>Humans</term>
<term>Internet</term>
<term>Polymerase Chain Reaction</term>
<term>Reproducibility of Results</term>
<term>Sequence Analysis, DNA</term>
</keywords>
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<term>Algorithmes</term>
<term>Analyse de regroupements</term>
<term>Analyse de séquence d'ADN</term>
<term>Bactéries</term>
<term>Humains</term>
<term>Internet</term>
<term>Métagénomique</term>
<term>Reproductibilité des résultats</term>
<term>Réaction de polymérisation en chaîne</term>
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<front>
<div type="abstract" xml:lang="en">Estimation of bacterial community composition from high-throughput sequenced 16S rRNA gene amplicons is a key task in microbial ecology. Since the sequence data from each sample typically consist of a large number of reads and are adversely impacted by different levels of biological and technical noise, accurate analysis of such large datasets is challenging.</div>
</front>
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<PMID Version="1">26496191</PMID>
<DateCompleted>
<Year>2016</Year>
<Month>06</Month>
<Day>15</Day>
</DateCompleted>
<DateRevised>
<Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
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<Journal>
<ISSN IssnType="Electronic">1932-6203</ISSN>
<JournalIssue CitedMedium="Internet">
<Volume>10</Volume>
<Issue>10</Issue>
<PubDate>
<Year>2015</Year>
</PubDate>
</JournalIssue>
<Title>PloS one</Title>
<ISOAbbreviation>PLoS ONE</ISOAbbreviation>
</Journal>
<ArticleTitle>ARK: Aggregation of Reads by K-Means for Estimation of Bacterial Community Composition.</ArticleTitle>
<Pagination>
<MedlinePgn>e0140644</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0140644</ELocationID>
<Abstract>
<AbstractText Label="MOTIVATION" NlmCategory="BACKGROUND">Estimation of bacterial community composition from high-throughput sequenced 16S rRNA gene amplicons is a key task in microbial ecology. Since the sequence data from each sample typically consist of a large number of reads and are adversely impacted by different levels of biological and technical noise, accurate analysis of such large datasets is challenging.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">There has been a recent surge of interest in using compressed sensing inspired and convex-optimization based methods to solve the estimation problem for bacterial community composition. These methods typically rely on summarizing the sequence data by frequencies of low-order k-mers and matching this information statistically with a taxonomically structured database. Here we show that the accuracy of the resulting community composition estimates can be substantially improved by aggregating the reads from a sample with an unsupervised machine learning approach prior to the estimation phase. The aggregation of reads is a pre-processing approach where we use a standard K-means clustering algorithm that partitions a large set of reads into subsets with reasonable computational cost to provide several vectors of first order statistics instead of only single statistical summarization in terms of k-mer frequencies. The output of the clustering is then processed further to obtain the final estimate for each sample. The resulting method is called Aggregation of Reads by K-means (ARK), and it is based on a statistical argument via mixture density formulation. ARK is found to improve the fidelity and robustness of several recently introduced methods, with only a modest increase in computational complexity.</AbstractText>
<AbstractText Label="AVAILABILITY" NlmCategory="BACKGROUND">An open source, platform-independent implementation of the method in the Julia programming language is freely available at https://github.com/dkoslicki/ARK. A Matlab implementation is available at http://www.ee.kth.se/ctsoftware.</AbstractText>
</Abstract>
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<Author ValidYN="Y">
<LastName>Koslicki</LastName>
<ForeName>David</ForeName>
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<AffiliationInfo>
<Affiliation>Dept of Mathematics, Oregon State University, Corvallis, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Chatterjee</LastName>
<ForeName>Saikat</ForeName>
<Initials>S</Initials>
<AffiliationInfo>
<Affiliation>Dept of Communication Theory, KTH Royal Institute of Technology, Stockholm, Sweden.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Shahrivar</LastName>
<ForeName>Damon</ForeName>
<Initials>D</Initials>
<AffiliationInfo>
<Affiliation>Dept of Communication Theory, KTH Royal Institute of Technology, Stockholm, Sweden.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Walker</LastName>
<ForeName>Alan W</ForeName>
<Initials>AW</Initials>
<AffiliationInfo>
<Affiliation>Microbiology Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Aberdeen, United Kingdom.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Francis</LastName>
<ForeName>Suzanna C</ForeName>
<Initials>SC</Initials>
<AffiliationInfo>
<Affiliation>MRC Tropical Epidemiology Group, London School of Hygiene and Tropical Medicine, London, United Kingdom.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Fraser</LastName>
<ForeName>Louise J</ForeName>
<Initials>LJ</Initials>
<AffiliationInfo>
<Affiliation>Illumina Cambridge Ltd., Chesterford Research Park, Essex, United Kingdom.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Vehkaperä</LastName>
<ForeName>Mikko</ForeName>
<Initials>M</Initials>
<AffiliationInfo>
<Affiliation>Dept of Electronic and Electrical Engineering, University of Sheffield, Sheffield, United Kingdom.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Lan</LastName>
<ForeName>Yueheng</ForeName>
<Initials>Y</Initials>
<AffiliationInfo>
<Affiliation>Dept of Physics, Tsinghua University, Beijing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Corander</LastName>
<ForeName>Jukka</ForeName>
<Initials>J</Initials>
<AffiliationInfo>
<Affiliation>Dept of Mathematics and Statistics, University of Helsinki, Helsinki, Finland.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y">
<Grant>
<Agency>Wellcome Trust</Agency>
<Country>United Kingdom</Country>
</Grant>
<Grant>
<GrantID>G0701039</GrantID>
<Agency>Medical Research Council</Agency>
<Country>United Kingdom</Country>
</Grant>
<Grant>
<GrantID>MR/K012126/1</GrantID>
<Agency>Medical Research Council</Agency>
<Country>United Kingdom</Country>
</Grant>
<Grant>
<GrantID>G1002369</GrantID>
<Agency>Medical Research Council</Agency>
<Country>United Kingdom</Country>
</Grant>
</GrantList>
<PublicationTypeList>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic">
<Year>2015</Year>
<Month>10</Month>
<Day>23</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo>
<Country>United States</Country>
<MedlineTA>PLoS One</MedlineTA>
<NlmUniqueID>101285081</NlmUniqueID>
<ISSNLinking>1932-6203</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList>
<MeshHeading>
<DescriptorName UI="D000465" MajorTopicYN="Y">Algorithms</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName>
<QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D016000" MajorTopicYN="N">Cluster Analysis</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D005243" MajorTopicYN="N">Feces</DescriptorName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D020407" MajorTopicYN="N">Internet</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D056186" MajorTopicYN="N">Metagenomics</DescriptorName>
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