MersV1, PubMed, Checkpoint, bibRecord, 001555

Evaluation of candidate vaccine approaches for MERS-CoV.

Identifieur interne : 001555 ( PubMed/Checkpoint ); précédent : 001554; suivant : 001556

Evaluation of candidate vaccine approaches for MERS-CoV.

Auteurs : Lingshu Wang [États-Unis] ; Wei Shi [États-Unis] ; M Gordon Joyce [États-Unis] ; Kayvon Modjarrad [États-Unis] ; Yi Zhang [États-Unis] ; Kwanyee Leung [États-Unis] ; Christopher R. Lees [États-Unis] ; Tongqing Zhou [États-Unis] ; Hadi M. Yassine [États-Unis] ; Masaru Kanekiyo [États-Unis] ; Zhi-Yong Yang [États-Unis] ; Xuejun Chen [États-Unis] ; Michelle M. Becker [États-Unis] ; Megan Freeman [États-Unis] ; Leatrice Vogel [États-Unis] ; Joshua C. Johnson [États-Unis] ; Gene Olinger [États-Unis] ; John P. Todd [États-Unis] ; Ulas Bagci [États-Unis] ; Jeffrey Solomon [États-Unis] ; Daniel J. Mollura [États-Unis] ; Lisa Hensley [États-Unis] ; Peter Jahrling [États-Unis] ; Mark R. Denison [États-Unis] ; Srinivas S. Rao [États-Unis] ; Kanta Subbarao [États-Unis] ; Peter D. Kwong [États-Unis] ; John R. Mascola [États-Unis] ; Wing-Pui Kong [États-Unis] ; Barney S. Graham [États-Unis]

Source :

Nature communications [ 2041-1723 ] ; 2015.

RBID : pubmed:26218507

Descripteurs français

English descriptors

KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Antibodies, Neutralizing (immunology), Antibodies, Viral (immunology), Coronavirus Infections (prevention & control), DNA, Viral (immunology), Female, HEK293 Cells, Humans, Immunoglobulin G (immunology), Macaca mulatta, Male, Mice, Mice, Inbred BALB C, Middle East Respiratory Syndrome Coronavirus (immunology), Spike Glycoprotein, Coronavirus (immunology), Vaccines, DNA (immunology), Viral Vaccines (immunology).
MESH :
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Neutralizing, Antibodies, Viral, DNA, Viral, Immunoglobulin G, Spike Glycoprotein, Coronavirus, Vaccines, DNA, Viral Vaccines.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- prevention & control : Coronavirus Infections.
- Animals, Female, HEK293 Cells, Humans, Macaca mulatta, Male, Mice, Mice, Inbred BALB C.

Abstract

The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) as a cause of severe respiratory disease highlights the need for effective approaches to CoV vaccine development. Efforts focused solely on the receptor-binding domain (RBD) of the viral Spike (S) glycoprotein may not optimize neutralizing antibody (NAb) responses. Here we show that immunogens based on full-length S DNA and S1 subunit protein elicit robust serum-neutralizing activity against several MERS-CoV strains in mice and non-human primates. Serological analysis and isolation of murine monoclonal antibodies revealed that immunization elicits NAbs to RBD and, non-RBD portions of S1 and S2 subunit. Multiple neutralization mechanisms were demonstrated by solving the atomic structure of a NAb-RBD complex, through sequencing of neutralization escape viruses and by constructing MERS-CoV S variants for serological assays. Immunization of rhesus macaques confers protection against MERS-CoV-induced radiographic pneumonia, as assessed using computerized tomography, supporting this strategy as a promising approach for MERS-CoV vaccine development.

DOI: 10.1038/ncomms8712
PubMed: 26218507

Affiliations:

États-Unis

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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Modjarrad, Kayvon" sort="Modjarrad, Kayvon" uniqKey="Modjarrad K" first="Kayvon" last="Modjarrad">Kayvon Modjarrad</name>
<affiliation wicri:level="1"><nlm:affiliation>1] Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA [2] U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA [3] Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland 20817, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>1] Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA [2] U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA [3] Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland 20817</wicri:regionArea>
<wicri:noRegion>Maryland 20817</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Yi" sort="Zhang, Yi" uniqKey="Zhang Y" first="Yi" last="Zhang">Yi Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Leung, Kwanyee" sort="Leung, Kwanyee" uniqKey="Leung K" first="Kwanyee" last="Leung">Kwanyee Leung</name>
<affiliation wicri:level="1"><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lees, Christopher R" sort="Lees, Christopher R" uniqKey="Lees C" first="Christopher R" last="Lees">Christopher R. Lees</name>
<affiliation wicri:level="1"><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhou, Tongqing" sort="Zhou, Tongqing" uniqKey="Zhou T" first="Tongqing" last="Zhou">Tongqing Zhou</name>
<affiliation wicri:level="1"><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yassine, Hadi M" sort="Yassine, Hadi M" uniqKey="Yassine H" first="Hadi M" last="Yassine">Hadi M. Yassine</name>
<affiliation wicri:level="1"><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kanekiyo, Masaru" sort="Kanekiyo, Masaru" uniqKey="Kanekiyo M" first="Masaru" last="Kanekiyo">Masaru Kanekiyo</name>
<affiliation wicri:level="1"><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yang, Zhi Yong" sort="Yang, Zhi Yong" uniqKey="Yang Z" first="Zhi-Yong" last="Yang">Zhi-Yong Yang</name>
<affiliation wicri:level="1"><nlm:affiliation>1] Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA [2] Sanofi-Aventis, 270 Albany Street, Cambridge, Massachusetts 02139, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>1] Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA [2] Sanofi-Aventis, 270 Albany Street, Cambridge, Massachusetts 02139</wicri:regionArea>
<wicri:noRegion>Massachusetts 02139</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Xuejun" sort="Chen, Xuejun" uniqKey="Chen X" first="Xuejun" last="Chen">Xuejun Chen</name>
<affiliation wicri:level="1"><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Becker, Michelle M" sort="Becker, Michelle M" uniqKey="Becker M" first="Michelle M" last="Becker">Michelle M. Becker</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232</wicri:regionArea>
<wicri:noRegion>Tennessee 37232</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Freeman, Megan" sort="Freeman, Megan" uniqKey="Freeman M" first="Megan" last="Freeman">Megan Freeman</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232</wicri:regionArea>
<wicri:noRegion>Tennessee 37232</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Vogel, Leatrice" sort="Vogel, Leatrice" uniqKey="Vogel L" first="Leatrice" last="Vogel">Leatrice Vogel</name>
<affiliation wicri:level="1"><nlm:affiliation>Emerging Respiratory Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Emerging Respiratory Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Johnson, Joshua C" sort="Johnson, Joshua C" uniqKey="Johnson J" first="Joshua C" last="Johnson">Joshua C. Johnson</name>
<affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland,21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland,21702</wicri:regionArea>
<wicri:noRegion>21702</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Olinger, Gene" sort="Olinger, Gene" uniqKey="Olinger G" first="Gene" last="Olinger">Gene Olinger</name>
<affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland,21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland,21702</wicri:regionArea>
<wicri:noRegion>21702</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Todd, John P" sort="Todd, John P" uniqKey="Todd J" first="John P" last="Todd">John P. Todd</name>
<affiliation wicri:level="1"><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bagci, Ulas" sort="Bagci, Ulas" uniqKey="Bagci U" first="Ulas" last="Bagci">Ulas Bagci</name>
<affiliation wicri:level="1"><nlm:affiliation>1] Center for Infectious Disease Imaging, Department of Radiology and Imaging Sciences, National Institutes of Health, Bethesda, Maryland 20892, USA [2] Center for Research in Computer Vision (CRCV), University of Central Florida, Orlando, Florida 32816, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>1] Center for Infectious Disease Imaging, Department of Radiology and Imaging Sciences, National Institutes of Health, Bethesda, Maryland 20892, USA [2] Center for Research in Computer Vision (CRCV), University of Central Florida, Orlando, Florida 32816</wicri:regionArea>
<wicri:noRegion>Florida 32816</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Solomon, Jeffrey" sort="Solomon, Jeffrey" uniqKey="Solomon J" first="Jeffrey" last="Solomon">Jeffrey Solomon</name>
<affiliation wicri:level="1"><nlm:affiliation>Center for Infectious Disease Imaging, Department of Radiology and Imaging Sciences, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Infectious Disease Imaging, Department of Radiology and Imaging Sciences, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mollura, Daniel J" sort="Mollura, Daniel J" uniqKey="Mollura D" first="Daniel J" last="Mollura">Daniel J. Mollura</name>
<affiliation wicri:level="1"><nlm:affiliation>Center for Infectious Disease Imaging, Department of Radiology and Imaging Sciences, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Infectious Disease Imaging, Department of Radiology and Imaging Sciences, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hensley, Lisa" sort="Hensley, Lisa" uniqKey="Hensley L" first="Lisa" last="Hensley">Lisa Hensley</name>
<affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland,21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland,21702</wicri:regionArea>
<wicri:noRegion>21702</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jahrling, Peter" sort="Jahrling, Peter" uniqKey="Jahrling P" first="Peter" last="Jahrling">Peter Jahrling</name>
<affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland,21702, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland,21702</wicri:regionArea>
<wicri:noRegion>21702</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Denison, Mark R" sort="Denison, Mark R" uniqKey="Denison M" first="Mark R" last="Denison">Mark R. Denison</name>
<affiliation wicri:level="1"><nlm:affiliation>1] Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA [2] Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>1] Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA [2] Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37232</wicri:regionArea>
<wicri:noRegion>Tennessee 37232</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rao, Srinivas S" sort="Rao, Srinivas S" uniqKey="Rao S" first="Srinivas S" last="Rao">Srinivas S. Rao</name>
<affiliation wicri:level="1"><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name>
<affiliation wicri:level="1"><nlm:affiliation>Emerging Respiratory Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Emerging Respiratory Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kwong, Peter D" sort="Kwong, Peter D" uniqKey="Kwong P" first="Peter D" last="Kwong">Peter D. Kwong</name>
<affiliation wicri:level="1"><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mascola, John R" sort="Mascola, John R" uniqKey="Mascola J" first="John R" last="Mascola">John R. Mascola</name>
<affiliation wicri:level="1"><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kong, Wing Pui" sort="Kong, Wing Pui" uniqKey="Kong W" first="Wing-Pui" last="Kong">Wing-Pui Kong</name>
<affiliation wicri:level="1"><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Graham, Barney S" sort="Graham, Barney S" uniqKey="Graham B" first="Barney S" last="Graham">Barney S. Graham</name>
<affiliation wicri:level="1"><nlm:affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892</wicri:regionArea>
<wicri:noRegion>Maryland 20892</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Nature communications</title>
<idno type="eISSN">2041-1723</idno>
<imprint><date when="2015" type="published">2015</date>
</imprint>
</series>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Monoclonal (immunology)</term>
<term>Antibodies, Neutralizing (immunology)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Coronavirus Infections (prevention & control)</term>
<term>DNA, Viral (immunology)</term>
<term>Female</term>
<term>HEK293 Cells</term>
<term>Humans</term>
<term>Immunoglobulin G (immunology)</term>
<term>Macaca mulatta</term>
<term>Male</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Middle East Respiratory Syndrome Coronavirus (immunology)</term>
<term>Spike Glycoprotein, Coronavirus (immunology)</term>
<term>Vaccines, DNA (immunology)</term>
<term>Viral Vaccines (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (immunologie)</term>
<term>Animaux</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps monoclonaux (immunologie)</term>
<term>Anticorps neutralisants (immunologie)</term>
<term>Cellules HEK293</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term>
<term>Femelle</term>
<term>Glycoprotéine de spicule des coronavirus (immunologie)</term>
<term>Humains</term>
<term>Immunoglobuline G (immunologie)</term>
<term>Infections à coronavirus ()</term>
<term>Macaca mulatta</term>
<term>Mâle</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Vaccins antiviraux (immunologie)</term>
<term>Vaccins à ADN (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term>
<term>Antibodies, Neutralizing</term>
<term>Antibodies, Viral</term>
<term>DNA, Viral</term>
<term>Immunoglobulin G</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vaccines, DNA</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>ADN viral</term>
<term>Anticorps antiviraux</term>
<term>Anticorps monoclonaux</term>
<term>Anticorps neutralisants</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Immunoglobuline G</term>
<term>Vaccins antiviraux</term>
<term>Vaccins à ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Female</term>
<term>HEK293 Cells</term>
<term>Humans</term>
<term>Macaca mulatta</term>
<term>Male</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules HEK293</term>
<term>Femelle</term>
<term>Humains</term>
<term>Infections à coronavirus</term>
<term>Macaca mulatta</term>
<term>Mâle</term>
<term>Souris</term>
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<front><div type="abstract" xml:lang="en">The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) as a cause of severe respiratory disease highlights the need for effective approaches to CoV vaccine development. Efforts focused solely on the receptor-binding domain (RBD) of the viral Spike (S) glycoprotein may not optimize neutralizing antibody (NAb) responses. Here we show that immunogens based on full-length S DNA and S1 subunit protein elicit robust serum-neutralizing activity against several MERS-CoV strains in mice and non-human primates. Serological analysis and isolation of murine monoclonal antibodies revealed that immunization elicits NAbs to RBD and, non-RBD portions of S1 and S2 subunit. Multiple neutralization mechanisms were demonstrated by solving the atomic structure of a NAb-RBD complex, through sequencing of neutralization escape viruses and by constructing MERS-CoV S variants for serological assays. Immunization of rhesus macaques confers protection against MERS-CoV-induced radiographic pneumonia, as assessed using computerized tomography, supporting this strategy as a promising approach for MERS-CoV vaccine development. </div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26218507</PMID>
<DateCompleted><Year>2016</Year>
<Month>04</Month>
<Day>15</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>01</Month>
<Day>08</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2041-1723</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>6</Volume>
<PubDate><Year>2015</Year>
<Month>Jul</Month>
<Day>28</Day>
</PubDate>
</JournalIssue>
<Title>Nature communications</Title>
<ISOAbbreviation>Nat Commun</ISOAbbreviation>
</Journal>
<ArticleTitle>Evaluation of candidate vaccine approaches for MERS-CoV.</ArticleTitle>
<Pagination><MedlinePgn>7712</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1038/ncomms8712</ELocationID>
<Abstract><AbstractText>The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) as a cause of severe respiratory disease highlights the need for effective approaches to CoV vaccine development. Efforts focused solely on the receptor-binding domain (RBD) of the viral Spike (S) glycoprotein may not optimize neutralizing antibody (NAb) responses. Here we show that immunogens based on full-length S DNA and S1 subunit protein elicit robust serum-neutralizing activity against several MERS-CoV strains in mice and non-human primates. Serological analysis and isolation of murine monoclonal antibodies revealed that immunization elicits NAbs to RBD and, non-RBD portions of S1 and S2 subunit. Multiple neutralization mechanisms were demonstrated by solving the atomic structure of a NAb-RBD complex, through sequencing of neutralization escape viruses and by constructing MERS-CoV S variants for serological assays. Immunization of rhesus macaques confers protection against MERS-CoV-induced radiographic pneumonia, as assessed using computerized tomography, supporting this strategy as a promising approach for MERS-CoV vaccine development. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Lingshu</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shi</LastName>
<ForeName>Wei</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Joyce</LastName>
<ForeName>M Gordon</ForeName>
<Initials>MG</Initials>
<AffiliationInfo><Affiliation>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Modjarrad</LastName>
<ForeName>Kayvon</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>1] Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA [2] U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA [3] Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland 20817, USA.</Affiliation>
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<name sortKey="Chen, Xuejun" sort="Chen, Xuejun" uniqKey="Chen X" first="Xuejun" last="Chen">Xuejun Chen</name>
<name sortKey="Denison, Mark R" sort="Denison, Mark R" uniqKey="Denison M" first="Mark R" last="Denison">Mark R. Denison</name>
<name sortKey="Freeman, Megan" sort="Freeman, Megan" uniqKey="Freeman M" first="Megan" last="Freeman">Megan Freeman</name>
<name sortKey="Graham, Barney S" sort="Graham, Barney S" uniqKey="Graham B" first="Barney S" last="Graham">Barney S. Graham</name>
<name sortKey="Hensley, Lisa" sort="Hensley, Lisa" uniqKey="Hensley L" first="Lisa" last="Hensley">Lisa Hensley</name>
<name sortKey="Jahrling, Peter" sort="Jahrling, Peter" uniqKey="Jahrling P" first="Peter" last="Jahrling">Peter Jahrling</name>
<name sortKey="Johnson, Joshua C" sort="Johnson, Joshua C" uniqKey="Johnson J" first="Joshua C" last="Johnson">Joshua C. Johnson</name>
<name sortKey="Joyce, M Gordon" sort="Joyce, M Gordon" uniqKey="Joyce M" first="M Gordon" last="Joyce">M Gordon Joyce</name>
<name sortKey="Kanekiyo, Masaru" sort="Kanekiyo, Masaru" uniqKey="Kanekiyo M" first="Masaru" last="Kanekiyo">Masaru Kanekiyo</name>
<name sortKey="Kong, Wing Pui" sort="Kong, Wing Pui" uniqKey="Kong W" first="Wing-Pui" last="Kong">Wing-Pui Kong</name>
<name sortKey="Kwong, Peter D" sort="Kwong, Peter D" uniqKey="Kwong P" first="Peter D" last="Kwong">Peter D. Kwong</name>
<name sortKey="Lees, Christopher R" sort="Lees, Christopher R" uniqKey="Lees C" first="Christopher R" last="Lees">Christopher R. Lees</name>
<name sortKey="Leung, Kwanyee" sort="Leung, Kwanyee" uniqKey="Leung K" first="Kwanyee" last="Leung">Kwanyee Leung</name>
<name sortKey="Mascola, John R" sort="Mascola, John R" uniqKey="Mascola J" first="John R" last="Mascola">John R. Mascola</name>
<name sortKey="Modjarrad, Kayvon" sort="Modjarrad, Kayvon" uniqKey="Modjarrad K" first="Kayvon" last="Modjarrad">Kayvon Modjarrad</name>
<name sortKey="Mollura, Daniel J" sort="Mollura, Daniel J" uniqKey="Mollura D" first="Daniel J" last="Mollura">Daniel J. Mollura</name>
<name sortKey="Olinger, Gene" sort="Olinger, Gene" uniqKey="Olinger G" first="Gene" last="Olinger">Gene Olinger</name>
<name sortKey="Rao, Srinivas S" sort="Rao, Srinivas S" uniqKey="Rao S" first="Srinivas S" last="Rao">Srinivas S. Rao</name>
<name sortKey="Shi, Wei" sort="Shi, Wei" uniqKey="Shi W" first="Wei" last="Shi">Wei Shi</name>
<name sortKey="Solomon, Jeffrey" sort="Solomon, Jeffrey" uniqKey="Solomon J" first="Jeffrey" last="Solomon">Jeffrey Solomon</name>
<name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name>
<name sortKey="Todd, John P" sort="Todd, John P" uniqKey="Todd J" first="John P" last="Todd">John P. Todd</name>
<name sortKey="Vogel, Leatrice" sort="Vogel, Leatrice" uniqKey="Vogel L" first="Leatrice" last="Vogel">Leatrice Vogel</name>
<name sortKey="Yang, Zhi Yong" sort="Yang, Zhi Yong" uniqKey="Yang Z" first="Zhi-Yong" last="Yang">Zhi-Yong Yang</name>
<name sortKey="Yassine, Hadi M" sort="Yassine, Hadi M" uniqKey="Yassine H" first="Hadi M" last="Yassine">Hadi M. Yassine</name>
<name sortKey="Zhang, Yi" sort="Zhang, Yi" uniqKey="Zhang Y" first="Yi" last="Zhang">Yi Zhang</name>
<name sortKey="Zhou, Tongqing" sort="Zhou, Tongqing" uniqKey="Zhou T" first="Tongqing" last="Zhou">Tongqing Zhou</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001555 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 001555 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    PubMed
   |étape=   Checkpoint
   |type=    RBID
   |clé=     pubmed:26218507
   |texte=   Evaluation of candidate vaccine approaches for MERS-CoV.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i   -Sk "pubmed:26218507" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

Evaluation of candidate vaccine approaches for MERS-CoV.

Evaluation of candidate vaccine approaches for MERS-CoV.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki