Generation and Characterization of Eptesicus fuscus (Big brown bat) kidney cell lines immortalized using the Myotis polyomavirus large T-antigen.
Identifieur interne : 001088 ( PubMed/Checkpoint ); précédent : 001087; suivant : 001089Generation and Characterization of Eptesicus fuscus (Big brown bat) kidney cell lines immortalized using the Myotis polyomavirus large T-antigen.
Auteurs : Arinjay Banerjee [Canada] ; Noreen Rapin [Canada] ; Megan Miller [États-Unis] ; Philip Griebel [Canada] ; Yan Zhou [Canada] ; Vincent Munster [États-Unis] ; Vikram Misra [Canada]Source :
- Journal of virological methods [ 1879-0984 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Antigènes transformants de polyomavirus, Cellules épithéliales (virologie), Chiroptera, Coronavirus du syndrome respiratoire du Moyen-Orient (croissance et développement), Fibroblastes (virologie), Kératines (génétique), Lignée cellulaire, Polyomavirus (croissance et développement), Polyomavirus (physiologie), Rein, Simplexvirus (croissance et développement), Techniques de culture cellulaire, Transformation cellulaire virale, Vesiculovirus (croissance et développement), Vimentine (génétique), Virus de la diarrhée porcine épidémique (croissance et développement).
- MESH :
- croissance et développement : Coronavirus du syndrome respiratoire du Moyen-Orient, Polyomavirus, Simplexvirus, Vesiculovirus, Virus de la diarrhée porcine épidémique.
- génétique : Kératines, Vimentine.
- physiologie : Polyomavirus.
- virologie : Cellules épithéliales, Fibroblastes.
- Animaux, Antigènes transformants de polyomavirus, Chiroptera, Lignée cellulaire, Rein, Techniques de culture cellulaire, Transformation cellulaire virale.
English descriptors
- KwdEn :
- Animals, Antigens, Polyomavirus Transforming, Cell Culture Techniques, Cell Line, Cell Transformation, Viral, Chiroptera, Epithelial Cells (virology), Fibroblasts (virology), Keratins (genetics), Kidney, Middle East Respiratory Syndrome Coronavirus (growth & development), Polyomavirus (growth & development), Polyomavirus (physiology), Porcine epidemic diarrhea virus (growth & development), Simplexvirus (growth & development), Vesiculovirus (growth & development), Vimentin (genetics).
- MESH :
- chemical , genetics : Keratins, Vimentin.
- chemical : Antigens, Polyomavirus Transforming.
- growth & development : Middle East Respiratory Syndrome Coronavirus, Polyomavirus, Porcine epidemic diarrhea virus, Simplexvirus, Vesiculovirus.
- physiology : Polyomavirus.
- virology : Epithelial Cells, Fibroblasts.
- Animals, Cell Culture Techniques, Cell Line, Cell Transformation, Viral, Chiroptera, Kidney.
Abstract
It is speculated that bats are important reservoir hosts for numerous viruses, with 27 viral families reportedly detected in bats. Majority of these viruses have not been isolated and there is little information regarding their biology in bats. Establishing a well-characterized bat cell line supporting the replication of bat-borne viruses would facilitate the analysis of virus-host interactions in an in vitro model. Currently, few bat cell lines have been developed and only Tb1-Lu, derived from Tadarida brasiliensis is commercially available. Here we describe a method to establish and immortalize big brown bat (Eptesicus fuscus) kidney (Efk3) cells using the Myotis polyomavirus T-antigen. Subclones of this cell line expressed both epithelial and fibroblast markers to varying extents. Cell clones expressed interferon beta in response to poly(I:C) stimulation and supported the replication of four different viruses, namely, vesicular stomatitis virus (VSV), porcine epidemic diarrhea coronavirus (PED-CoV), Middle-East respiratory syndrome coronavirus (MERS-CoV) and herpes simplex virus (HSV). To our knowledge, this is the first bat cell line from a northern latitude insectivorous bat developed using a novel technology. The cell line has the potential to be used for isolation of bat viruses and for studying virus-bat interactions in culture.
DOI: 10.1016/j.jviromet.2016.09.008
PubMed: 27639955
Affiliations:
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pubmed:27639955Le document en format XML
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<front><div type="abstract" xml:lang="en">It is speculated that bats are important reservoir hosts for numerous viruses, with 27 viral families reportedly detected in bats. Majority of these viruses have not been isolated and there is little information regarding their biology in bats. Establishing a well-characterized bat cell line supporting the replication of bat-borne viruses would facilitate the analysis of virus-host interactions in an in vitro model. Currently, few bat cell lines have been developed and only Tb1-Lu, derived from Tadarida brasiliensis is commercially available. Here we describe a method to establish and immortalize big brown bat (Eptesicus fuscus) kidney (Efk3) cells using the Myotis polyomavirus T-antigen. Subclones of this cell line expressed both epithelial and fibroblast markers to varying extents. Cell clones expressed interferon beta in response to poly(I:C) stimulation and supported the replication of four different viruses, namely, vesicular stomatitis virus (VSV), porcine epidemic diarrhea coronavirus (PED-CoV), Middle-East respiratory syndrome coronavirus (MERS-CoV) and herpes simplex virus (HSV). To our knowledge, this is the first bat cell line from a northern latitude insectivorous bat developed using a novel technology. The cell line has the potential to be used for isolation of bat viruses and for studying virus-bat interactions in culture.</div>
</front>
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<Abstract><AbstractText>It is speculated that bats are important reservoir hosts for numerous viruses, with 27 viral families reportedly detected in bats. Majority of these viruses have not been isolated and there is little information regarding their biology in bats. Establishing a well-characterized bat cell line supporting the replication of bat-borne viruses would facilitate the analysis of virus-host interactions in an in vitro model. Currently, few bat cell lines have been developed and only Tb1-Lu, derived from Tadarida brasiliensis is commercially available. Here we describe a method to establish and immortalize big brown bat (Eptesicus fuscus) kidney (Efk3) cells using the Myotis polyomavirus T-antigen. Subclones of this cell line expressed both epithelial and fibroblast markers to varying extents. Cell clones expressed interferon beta in response to poly(I:C) stimulation and supported the replication of four different viruses, namely, vesicular stomatitis virus (VSV), porcine epidemic diarrhea coronavirus (PED-CoV), Middle-East respiratory syndrome coronavirus (MERS-CoV) and herpes simplex virus (HSV). To our knowledge, this is the first bat cell line from a northern latitude insectivorous bat developed using a novel technology. The cell line has the potential to be used for isolation of bat viruses and for studying virus-bat interactions in culture.</AbstractText>
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</ArticleIdList>
<ReferenceList><Reference><Citation>J Virol. 2010 Dec;84(24):13004-18</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20926577</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Exp Cell Res. 1995 Sep;220(1):161-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7664832</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Springerplus. 2014 Oct 08;3:588</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25332888</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Int J Legal Med. 2000;114(1-2):23-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11197623</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS One. 2014 Oct 08;9(10):e109795</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25295526</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 2007 Mar;88(Pt 3):956-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17325370</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Exp Cell Res. 1994 Sep;214(1):270-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8082730</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Science. 2013 Jan 25;339(6118):456-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23258410</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Zoonoses Public Health. 2013 Feb;60(1):104-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23302292</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Appl Microbiol Biotechnol. 2015 Nov;99(21):9135-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26156242</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Neurosci Methods. 2009 Jan 30;176(2):112-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18822316</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Commun. 2012 Apr 24;3:796</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22531181</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virol J. 2015 Dec 22;12:193</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26689811</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Clin Microbiol. 2003 May;41(5):1850-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12734216</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS One. 2014 Sep 09;9(9):e107395</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25203391</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Oncogene. 2005 Nov 21;24(52):7729-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16299533</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Exp Cell Res. 1986 Oct;166(2):391-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2427348</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Theriogenology. 2009 Jun;71(9):1417-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19303628</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Leukoc Biol. 2013 Nov;94(5):1025-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23911868</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 2009 Aug;90(Pt 8):2015-2022</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19357225</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Appl Environ Microbiol. 2001 Jul;67(7):3195-200</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11425741</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Rev Immunol. 2002 Sep;2(9):675-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12209136</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2010 May 11;107(19):8666-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20421465</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Annu Rev Microbiol. 2012;66:213-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22994493</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS One. 2009 Dec 11;4(12):e8266</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20011515</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS One. 2011;6(11):e28131</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22140523</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Exp Cell Res. 1986 Feb;162(2):530-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3002824</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Vis Exp. 2014 Jan 28;(83):e50203</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24513647</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2014 May;88(10):5209-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24600003</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virchows Arch A Pathol Anat Histopathol. 1988;413(2):91-101</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2455381</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol Methods. 2006 Jul;135(1):56-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16516983</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS One. 2014 Nov 12;9(11):e112285</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25391018</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2014 Jan;88(1):99-109</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24131711</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Mem Inst Oswaldo Cruz. 2015 Feb;110(1):1-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25742261</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS One. 2013 Oct 03;8(10):e76469</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24098509</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virol J. 2015 Apr 11;12:57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25884446</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS One. 2011;6(7):e22488</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21811620</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Microbiol Immunol. 2014 Sep;58(9):483-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25040642</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Viruses. 2012 May;4(5):889-900</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22754654</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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