Generation and Characterization of Eptesicus fuscus (Big brown bat) kidney cell lines immortalized using the Myotis polyomavirus large T-antigen.
Identifieur interne : 001088 ( PubMed/Checkpoint ); précédent : 001087; suivant : 001089Generation and Characterization of Eptesicus fuscus (Big brown bat) kidney cell lines immortalized using the Myotis polyomavirus large T-antigen.
Auteurs : Arinjay Banerjee [Canada] ; Noreen Rapin [Canada] ; Megan Miller [États-Unis] ; Philip Griebel [Canada] ; Yan Zhou [Canada] ; Vincent Munster [États-Unis] ; Vikram Misra [Canada]Source :
- Journal of virological methods [ 1879-0984 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Antigènes transformants de polyomavirus, Cellules épithéliales (virologie), Chiroptera, Coronavirus du syndrome respiratoire du Moyen-Orient (croissance et développement), Fibroblastes (virologie), Kératines (génétique), Lignée cellulaire, Polyomavirus (croissance et développement), Polyomavirus (physiologie), Rein, Simplexvirus (croissance et développement), Techniques de culture cellulaire, Transformation cellulaire virale, Vesiculovirus (croissance et développement), Vimentine (génétique), Virus de la diarrhée porcine épidémique (croissance et développement).
- MESH :
- croissance et développement : Coronavirus du syndrome respiratoire du Moyen-Orient, Polyomavirus, Simplexvirus, Vesiculovirus, Virus de la diarrhée porcine épidémique.
- génétique : Kératines, Vimentine.
- physiologie : Polyomavirus.
- virologie : Cellules épithéliales, Fibroblastes.
- Animaux, Antigènes transformants de polyomavirus, Chiroptera, Lignée cellulaire, Rein, Techniques de culture cellulaire, Transformation cellulaire virale.
English descriptors
- KwdEn :
- Animals, Antigens, Polyomavirus Transforming, Cell Culture Techniques, Cell Line, Cell Transformation, Viral, Chiroptera, Epithelial Cells (virology), Fibroblasts (virology), Keratins (genetics), Kidney, Middle East Respiratory Syndrome Coronavirus (growth & development), Polyomavirus (growth & development), Polyomavirus (physiology), Porcine epidemic diarrhea virus (growth & development), Simplexvirus (growth & development), Vesiculovirus (growth & development), Vimentin (genetics).
- MESH :
- chemical , genetics : Keratins, Vimentin.
- chemical : Antigens, Polyomavirus Transforming.
- growth & development : Middle East Respiratory Syndrome Coronavirus, Polyomavirus, Porcine epidemic diarrhea virus, Simplexvirus, Vesiculovirus.
- physiology : Polyomavirus.
- virology : Epithelial Cells, Fibroblasts.
- Animals, Cell Culture Techniques, Cell Line, Cell Transformation, Viral, Chiroptera, Kidney.
Abstract
It is speculated that bats are important reservoir hosts for numerous viruses, with 27 viral families reportedly detected in bats. Majority of these viruses have not been isolated and there is little information regarding their biology in bats. Establishing a well-characterized bat cell line supporting the replication of bat-borne viruses would facilitate the analysis of virus-host interactions in an in vitro model. Currently, few bat cell lines have been developed and only Tb1-Lu, derived from Tadarida brasiliensis is commercially available. Here we describe a method to establish and immortalize big brown bat (Eptesicus fuscus) kidney (Efk3) cells using the Myotis polyomavirus T-antigen. Subclones of this cell line expressed both epithelial and fibroblast markers to varying extents. Cell clones expressed interferon beta in response to poly(I:C) stimulation and supported the replication of four different viruses, namely, vesicular stomatitis virus (VSV), porcine epidemic diarrhea coronavirus (PED-CoV), Middle-East respiratory syndrome coronavirus (MERS-CoV) and herpes simplex virus (HSV). To our knowledge, this is the first bat cell line from a northern latitude insectivorous bat developed using a novel technology. The cell line has the potential to be used for isolation of bat viruses and for studying virus-bat interactions in culture.
DOI: 10.1016/j.jviromet.2016.09.008
PubMed: 27639955
Affiliations:
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International Vaccine Center (VIDO-InterVac), University of Saskatchewan, Saskatoon, Saskatchewan, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Vaccine and Infectious Disease Organization - International Vaccine Center (VIDO-InterVac), University of Saskatchewan, Saskatoon, Saskatchewan</wicri:regionArea><wicri:noRegion>Saskatchewan</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Yan" sort="Zhou, Yan" uniqKey="Zhou Y" first="Yan" last="Zhou">Yan Zhou</name><affiliation wicri:level="1"><nlm:affiliation>Vaccine and Infectious Disease Organization - International Vaccine Center (VIDO-InterVac), University of Saskatchewan, Saskatoon, Saskatchewan, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Vaccine and Infectious Disease Organization - International Vaccine Center (VIDO-InterVac), University of Saskatchewan, Saskatoon, Saskatchewan</wicri:regionArea><wicri:noRegion>Saskatchewan</wicri:noRegion></affiliation></author><author><name sortKey="Munster, Vincent" sort="Munster, Vincent" uniqKey="Munster V" first="Vincent" last="Munster">Vincent Munster</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Misra, Vikram" sort="Misra, Vikram" uniqKey="Misra V" first="Vikram" last="Misra">Vikram Misra</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. Electronic address: vikram.misra@usask.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan</wicri:regionArea><wicri:noRegion>Saskatchewan</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of virological methods</title><idno type="eISSN">1879-0984</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, Polyomavirus Transforming</term><term>Cell Culture Techniques</term><term>Cell Line</term><term>Cell Transformation, Viral</term><term>Chiroptera</term><term>Epithelial Cells (virology)</term><term>Fibroblasts (virology)</term><term>Keratins (genetics)</term><term>Kidney</term><term>Middle East Respiratory Syndrome Coronavirus (growth & development)</term><term>Polyomavirus (growth & development)</term><term>Polyomavirus (physiology)</term><term>Porcine epidemic diarrhea virus (growth & development)</term><term>Simplexvirus (growth & development)</term><term>Vesiculovirus (growth & development)</term><term>Vimentin (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antigènes transformants de polyomavirus</term><term>Cellules épithéliales (virologie)</term><term>Chiroptera</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (croissance et développement)</term><term>Fibroblastes (virologie)</term><term>Kératines (génétique)</term><term>Lignée cellulaire</term><term>Polyomavirus (croissance et développement)</term><term>Polyomavirus (physiologie)</term><term>Rein</term><term>Simplexvirus (croissance et développement)</term><term>Techniques de culture cellulaire</term><term>Transformation cellulaire virale</term><term>Vesiculovirus (croissance et développement)</term><term>Vimentine (génétique)</term><term>Virus de la diarrhée porcine épidémique (croissance et développement)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Keratins</term><term>Vimentin</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antigens, Polyomavirus Transforming</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Polyomavirus</term><term>Simplexvirus</term><term>Vesiculovirus</term><term>Virus de la diarrhée porcine épidémique</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term><term>Polyomavirus</term><term>Porcine epidemic diarrhea virus</term><term>Simplexvirus</term><term>Vesiculovirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Kératines</term><term>Vimentine</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Polyomavirus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Polyomavirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term><term>Fibroblastes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term><term>Fibroblasts</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Culture Techniques</term><term>Cell Line</term><term>Cell Transformation, Viral</term><term>Chiroptera</term><term>Kidney</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Antigènes transformants de polyomavirus</term><term>Chiroptera</term><term>Lignée cellulaire</term><term>Rein</term><term>Techniques de culture cellulaire</term><term>Transformation cellulaire virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">It is speculated that bats are important reservoir hosts for numerous viruses, with 27 viral families reportedly detected in bats. Majority of these viruses have not been isolated and there is little information regarding their biology in bats. Establishing a well-characterized bat cell line supporting the replication of bat-borne viruses would facilitate the analysis of virus-host interactions in an in vitro model. Currently, few bat cell lines have been developed and only Tb1-Lu, derived from Tadarida brasiliensis is commercially available. Here we describe a method to establish and immortalize big brown bat (Eptesicus fuscus) kidney (Efk3) cells using the Myotis polyomavirus T-antigen. Subclones of this cell line expressed both epithelial and fibroblast markers to varying extents. Cell clones expressed interferon beta in response to poly(I:C) stimulation and supported the replication of four different viruses, namely, vesicular stomatitis virus (VSV), porcine epidemic diarrhea coronavirus (PED-CoV), Middle-East respiratory syndrome coronavirus (MERS-CoV) and herpes simplex virus (HSV). To our knowledge, this is the first bat cell line from a northern latitude insectivorous bat developed using a novel technology. The cell line has the potential to be used for isolation of bat viruses and for studying virus-bat interactions in culture.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27639955</PMID><DateCompleted><Year>2017</Year><Month>07</Month><Day>31</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0984</ISSN><JournalIssue CitedMedium="Internet"><Volume>237</Volume><PubDate><Year>2016</Year><Month>11</Month></PubDate></JournalIssue><Title>Journal of virological methods</Title><ISOAbbreviation>J. Virol. Methods</ISOAbbreviation></Journal><ArticleTitle>Generation and Characterization of Eptesicus fuscus (Big brown bat) kidney cell lines immortalized using the Myotis polyomavirus large T-antigen.</ArticleTitle><Pagination><MedlinePgn>166-173</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0166-0934(16)30244-0</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jviromet.2016.09.008</ELocationID><Abstract><AbstractText>It is speculated that bats are important reservoir hosts for numerous viruses, with 27 viral families reportedly detected in bats. Majority of these viruses have not been isolated and there is little information regarding their biology in bats. Establishing a well-characterized bat cell line supporting the replication of bat-borne viruses would facilitate the analysis of virus-host interactions in an in vitro model. Currently, few bat cell lines have been developed and only Tb1-Lu, derived from Tadarida brasiliensis is commercially available. Here we describe a method to establish and immortalize big brown bat (Eptesicus fuscus) kidney (Efk3) cells using the Myotis polyomavirus T-antigen. Subclones of this cell line expressed both epithelial and fibroblast markers to varying extents. Cell clones expressed interferon beta in response to poly(I:C) stimulation and supported the replication of four different viruses, namely, vesicular stomatitis virus (VSV), porcine epidemic diarrhea coronavirus (PED-CoV), Middle-East respiratory syndrome coronavirus (MERS-CoV) and herpes simplex virus (HSV). To our knowledge, this is the first bat cell line from a northern latitude insectivorous bat developed using a novel technology. The cell line has the potential to be used for isolation of bat viruses and for studying virus-bat interactions in culture.</AbstractText><CopyrightInformation>Copyright © 2016 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Banerjee</LastName><ForeName>Arinjay</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rapin</LastName><ForeName>Noreen</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miller</LastName><ForeName>Megan</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Griebel</LastName><ForeName>Philip</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Vaccine and Infectious Disease Organization - International Vaccine Center (VIDO-InterVac), University of Saskatchewan, Saskatoon, Saskatchewan, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Yan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Vaccine and Infectious Disease Organization - International Vaccine Center (VIDO-InterVac), University of Saskatchewan, Saskatoon, Saskatchewan, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Munster</LastName><ForeName>Vincent</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Misra</LastName><ForeName>Vikram</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. Electronic address: vikram.misra@usask.ca.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D052060">Research Support, N.I.H., Intramural</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>09</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Virol Methods</MedlineTA><NlmUniqueID>8005839</NlmUniqueID><ISSNLinking>0166-0934</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000952">Antigens, Polyomavirus Transforming</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014746">Vimentin</NameOfSubstance></Chemical><Chemical><RegistryNumber>68238-35-7</RegistryNumber><NameOfSubstance UI="D007633">Keratins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000952" MajorTopicYN="Y">Antigens, Polyomavirus Transforming</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018929" MajorTopicYN="N">Cell Culture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="Y">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002472" MajorTopicYN="Y">Cell Transformation, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002685" MajorTopicYN="Y">Chiroptera</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004847" MajorTopicYN="N">Epithelial Cells</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005347" MajorTopicYN="N">Fibroblasts</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007633" MajorTopicYN="N">Keratins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007668" MajorTopicYN="Y">Kidney</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011120" MajorTopicYN="N">Polyomavirus</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName 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