One-Pot Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) for Detecting MERS-CoV.
Identifieur interne : 000F57 ( PubMed/Checkpoint ); précédent : 000F56; suivant : 000F58One-Pot Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) for Detecting MERS-CoV.
Auteurs : Se Hee Lee [Corée du Sud] ; Yun Hee Baek [Corée du Sud] ; Yang-Hoon Kim [Corée du Sud] ; Young-Ki Choi [Corée du Sud] ; Min-Suk Song [Corée du Sud] ; Ji-Young Ahn [Corée du Sud]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2016.
Abstract
Due to the limitation of rapid development of specific antiviral drug or vaccine for novel emerging viruses, an accurate and rapid diagnosis is a key to manage the virus spread. We developed an efficient and rapid method with high specificity for the Middle East Respiratory Syndrome coronavirus (MERS-CoV), based on one-pot reverse transcription loop-mediated isothermal amplification (one-pot RT-LAMP). A set of six LAMP primers [F3, B3, FIP, BIP, LF (Loop-F), and LB (Loop-B)] were designed using the sequence of nucleocapsid (N) gene with optimized RT-LAMP enzyme conditions: 100 U M-MLV RTase and 4 U Bst polymerase, implying that the reaction was able to detect four infectious viral genome copies of MERS-CoV within a 60 min reaction time period. Significantly, EvaGreen dye has better signal read-out properties in one-pot RT-LAMP reaction and is more compatible with DNA polymerase than SYBR green I. Isothermally amplified specific N genes were further evaluated using field-deployable microchamber devices, leading to the specific identification of as few as 0.4 infectious viral genome copies, with no cross-reaction to the other acute respiratory disease viruses, including influenza type A (H1N1 and H3N2), type B, human coronavirus 229E, and human metapneumovirus. This sensitive, specific and feasible method provides a large-scale technical support in emergencies, and is also applied as a sample-to-detection module in Point of Care Testing devices.
DOI: 10.3389/fmicb.2016.02166
PubMed: 28119682
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:28119682Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">One-Pot Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) for Detecting MERS-CoV.</title><author><name sortKey="Lee, Se Hee" sort="Lee, Se Hee" uniqKey="Lee S" first="Se Hee" last="Lee">Se Hee Lee</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Chungbuk National University Cheongju, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>School of Biological Sciences, Chungbuk National University Cheongju</wicri:regionArea><wicri:noRegion>Chungbuk National University Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Baek, Yun Hee" sort="Baek, Yun Hee" uniqKey="Baek Y" first="Yun Hee" last="Baek">Yun Hee Baek</name><affiliation wicri:level="1"><nlm:affiliation>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju</wicri:regionArea><wicri:noRegion>Chungbuk National University Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Yang Hoon" sort="Kim, Yang Hoon" uniqKey="Kim Y" first="Yang-Hoon" last="Kim">Yang-Hoon Kim</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Chungbuk National University Cheongju, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>School of Biological Sciences, Chungbuk National University Cheongju</wicri:regionArea><wicri:noRegion>Chungbuk National University Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Choi, Young Ki" sort="Choi, Young Ki" uniqKey="Choi Y" first="Young-Ki" last="Choi">Young-Ki Choi</name><affiliation wicri:level="1"><nlm:affiliation>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju</wicri:regionArea><wicri:noRegion>Chungbuk National University Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Song, Min Suk" sort="Song, Min Suk" uniqKey="Song M" first="Min-Suk" last="Song">Min-Suk Song</name><affiliation wicri:level="1"><nlm:affiliation>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju</wicri:regionArea><wicri:noRegion>Chungbuk National University Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Ahn, Ji Young" sort="Ahn, Ji Young" uniqKey="Ahn J" first="Ji-Young" last="Ahn">Ji-Young Ahn</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Chungbuk National University Cheongju, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>School of Biological Sciences, Chungbuk National University Cheongju</wicri:regionArea><wicri:noRegion>Chungbuk National University Cheongju</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:28119682</idno><idno type="pmid">28119682</idno><idno type="doi">10.3389/fmicb.2016.02166</idno><idno type="wicri:Area/PubMed/Corpus">000E06</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000E06</idno><idno type="wicri:Area/PubMed/Curation">000E06</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000E06</idno><idno type="wicri:Area/PubMed/Checkpoint">000F57</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000F57</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">One-Pot Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) for Detecting MERS-CoV.</title><author><name sortKey="Lee, Se Hee" sort="Lee, Se Hee" uniqKey="Lee S" first="Se Hee" last="Lee">Se Hee Lee</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Chungbuk National University Cheongju, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>School of Biological Sciences, Chungbuk National University Cheongju</wicri:regionArea><wicri:noRegion>Chungbuk National University Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Baek, Yun Hee" sort="Baek, Yun Hee" uniqKey="Baek Y" first="Yun Hee" last="Baek">Yun Hee Baek</name><affiliation wicri:level="1"><nlm:affiliation>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju</wicri:regionArea><wicri:noRegion>Chungbuk National University Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Yang Hoon" sort="Kim, Yang Hoon" uniqKey="Kim Y" first="Yang-Hoon" last="Kim">Yang-Hoon Kim</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Chungbuk National University Cheongju, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>School of Biological Sciences, Chungbuk National University Cheongju</wicri:regionArea><wicri:noRegion>Chungbuk National University Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Choi, Young Ki" sort="Choi, Young Ki" uniqKey="Choi Y" first="Young-Ki" last="Choi">Young-Ki Choi</name><affiliation wicri:level="1"><nlm:affiliation>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju</wicri:regionArea><wicri:noRegion>Chungbuk National University Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Song, Min Suk" sort="Song, Min Suk" uniqKey="Song M" first="Min-Suk" last="Song">Min-Suk Song</name><affiliation wicri:level="1"><nlm:affiliation>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju</wicri:regionArea><wicri:noRegion>Chungbuk National University Cheongju</wicri:noRegion></affiliation></author><author><name sortKey="Ahn, Ji Young" sort="Ahn, Ji Young" uniqKey="Ahn J" first="Ji-Young" last="Ahn">Ji-Young Ahn</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Chungbuk National University Cheongju, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>School of Biological Sciences, Chungbuk National University Cheongju</wicri:regionArea><wicri:noRegion>Chungbuk National University Cheongju</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Due to the limitation of rapid development of specific antiviral drug or vaccine for novel emerging viruses, an accurate and rapid diagnosis is a key to manage the virus spread. We developed an efficient and rapid method with high specificity for the Middle East Respiratory Syndrome coronavirus (MERS-CoV), based on one-pot reverse transcription loop-mediated isothermal amplification (one-pot RT-LAMP). A set of six LAMP primers [F3, B3, FIP, BIP, LF (Loop-F), and LB (Loop-B)] were designed using the sequence of nucleocapsid (N) gene with optimized RT-LAMP enzyme conditions: 100 U M-MLV RTase and 4 U <i>Bst</i> polymerase, implying that the reaction was able to detect four infectious viral genome copies of MERS-CoV within a 60 min reaction time period. Significantly, EvaGreen dye has better signal read-out properties in one-pot RT-LAMP reaction and is more compatible with DNA polymerase than SYBR green I. Isothermally amplified specific N genes were further evaluated using field-deployable microchamber devices, leading to the specific identification of as few as 0.4 infectious viral genome copies, with no cross-reaction to the other acute respiratory disease viruses, including influenza type A (H1N1 and H3N2), type B, human coronavirus 229E, and human metapneumovirus. This sensitive, specific and feasible method provides a large-scale technical support in emergencies, and is also applied as a sample-to-detection module in Point of Care Testing devices.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">28119682</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>One-Pot Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) for Detecting MERS-CoV.</ArticleTitle><Pagination><MedlinePgn>2166</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2016.02166</ELocationID><Abstract><AbstractText>Due to the limitation of rapid development of specific antiviral drug or vaccine for novel emerging viruses, an accurate and rapid diagnosis is a key to manage the virus spread. We developed an efficient and rapid method with high specificity for the Middle East Respiratory Syndrome coronavirus (MERS-CoV), based on one-pot reverse transcription loop-mediated isothermal amplification (one-pot RT-LAMP). A set of six LAMP primers [F3, B3, FIP, BIP, LF (Loop-F), and LB (Loop-B)] were designed using the sequence of nucleocapsid (N) gene with optimized RT-LAMP enzyme conditions: 100 U M-MLV RTase and 4 U <i>Bst</i> polymerase, implying that the reaction was able to detect four infectious viral genome copies of MERS-CoV within a 60 min reaction time period. Significantly, EvaGreen dye has better signal read-out properties in one-pot RT-LAMP reaction and is more compatible with DNA polymerase than SYBR green I. Isothermally amplified specific N genes were further evaluated using field-deployable microchamber devices, leading to the specific identification of as few as 0.4 infectious viral genome copies, with no cross-reaction to the other acute respiratory disease viruses, including influenza type A (H1N1 and H3N2), type B, human coronavirus 229E, and human metapneumovirus. This sensitive, specific and feasible method provides a large-scale technical support in emergencies, and is also applied as a sample-to-detection module in Point of Care Testing devices.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Se Hee</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>School of Biological Sciences, Chungbuk National University Cheongju, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Baek</LastName><ForeName>Yun Hee</ForeName><Initials>YH</Initials><AffiliationInfo><Affiliation>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Yang-Hoon</ForeName><Initials>YH</Initials><AffiliationInfo><Affiliation>School of Biological Sciences, Chungbuk National University Cheongju, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Young-Ki</ForeName><Initials>YK</Initials><AffiliationInfo><Affiliation>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Min-Suk</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>College of Medicine and Medical Research Institute, Chungbuk National University Cheongju, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ahn</LastName><ForeName>Ji-Young</ForeName><Initials>JY</Initials><AffiliationInfo><Affiliation>School of Biological Sciences, Chungbuk National University Cheongju, South Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>01</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Microbiol</MedlineTA><NlmUniqueID>101548977</NlmUniqueID><ISSNLinking>1664-302X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">EvaGreen</Keyword><Keyword MajorTopicYN="N">MERS-CoV</Keyword><Keyword MajorTopicYN="N">loop-mediated isothermal amplification (LAMP)</Keyword><Keyword MajorTopicYN="N">nucleocapsid (N) protein</Keyword><Keyword MajorTopicYN="N">one-pot reverse transcription (RT)</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>10</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>12</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28119682</ArticleId><ArticleId IdType="doi">10.3389/fmicb.2016.02166</ArticleId><ArticleId IdType="pmc">PMC5220095</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Euro Surveill. 2012 Sep 27;17(39):null</Citation><ArticleIdList><ArticleId IdType="pubmed">23041020</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Biotechnol. 2007 Nov 09;7:76</Citation><ArticleIdList><ArticleId IdType="pubmed">17996102</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2012 Nov 8;367 (19):1814-20</Citation><ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2009 Feb;37(2):473-81</Citation><ArticleIdList><ArticleId IdType="pubmed">19056821</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Virol. 2006 Oct;37(2):128-33</Citation><ArticleIdList><ArticleId IdType="pubmed">16973412</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2004 May;42(5):1956-61</Citation><ArticleIdList><ArticleId IdType="pubmed">15131154</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2005 Oct 28;310(5748):676-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16195424</ArticleId></ArticleIdList></Reference><Reference><Citation>Rev Med Virol. 2008 Nov-Dec;18(6):407-21</Citation><ArticleIdList><ArticleId IdType="pubmed">18716992</ArticleId></ArticleIdList></Reference><Reference><Citation>MMWR Morb Mortal Wkly Rep. 2014 May 16;63(19):431-6</Citation><ArticleIdList><ArticleId IdType="pubmed">24827411</ArticleId></ArticleIdList></Reference><Reference><Citation>Virol J. 2016 Jun 03;13:87</Citation><ArticleIdList><ArticleId IdType="pubmed">27255185</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2013 Jun 13;18(24):null</Citation><ArticleIdList><ArticleId IdType="pubmed">23787161</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Virol. 2008 Apr;41(4):270-6</Citation><ArticleIdList><ArticleId IdType="pubmed">18296109</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2003 Dec;41(12):5517-24</Citation><ArticleIdList><ArticleId IdType="pubmed">14662933</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2014 Feb 25;5(2):e00884-14</Citation><ArticleIdList><ArticleId IdType="pubmed">24570370</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Infect Dis. 2016 Mar 15;62(6):755-60</Citation><ArticleIdList><ArticleId IdType="pubmed">26679623</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2005 Jul;43(7):3290-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16000450</ArticleId></ArticleIdList></Reference><Reference><Citation>Lab Chip. 2006 Jan;6(1):46-53</Citation><ArticleIdList><ArticleId IdType="pubmed">16372068</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Virol. 2014 Jun;60(2):168-71</Citation><ArticleIdList><ArticleId IdType="pubmed">24726679</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2007;35(13):4223-37</Citation><ArticleIdList><ArticleId IdType="pubmed">17576684</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2015 Apr 09;10(4):e0123126</Citation><ArticleIdList><ArticleId IdType="pubmed">25856093</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2005 Jan;43(1):427-30</Citation><ArticleIdList><ArticleId IdType="pubmed">15635005</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2010 Mar;84(6):2808-19</Citation><ArticleIdList><ArticleId IdType="pubmed">20071579</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Curr. 2013 Dec 12;5:null</Citation><ArticleIdList><ArticleId IdType="pubmed">24459611</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 2012 Aug;157(8):1463-9</Citation><ArticleIdList><ArticleId IdType="pubmed">22566005</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2012 Nov 20;3(6):null</Citation><ArticleIdList><ArticleId IdType="pubmed">23170002</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Infect Dis. 2014 Dec;29:307-8</Citation><ArticleIdList><ArticleId IdType="pubmed">25448335</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biotechnol. 2006 Oct 1;125(4):484-91</Citation><ArticleIdList><ArticleId IdType="pubmed">16790287</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2005 Jun;43(6):2895-903</Citation><ArticleIdList><ArticleId IdType="pubmed">15956414</ArticleId></ArticleIdList></Reference><Reference><Citation>Virol J. 2014 Aug 08;11:139</Citation><ArticleIdList><ArticleId IdType="pubmed">25103205</ArticleId></ArticleIdList></Reference><Reference><Citation>Virol J. 2010 Jan 21;7:14</Citation><ArticleIdList><ArticleId IdType="pubmed">20092637</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2016 Mar 22;11(3):e0151769</Citation><ArticleIdList><ArticleId IdType="pubmed">27003504</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Lee, Se Hee" sort="Lee, Se Hee" uniqKey="Lee S" first="Se Hee" last="Lee">Se Hee Lee</name></noRegion><name sortKey="Ahn, Ji Young" sort="Ahn, Ji Young" uniqKey="Ahn J" first="Ji-Young" last="Ahn">Ji-Young Ahn</name><name sortKey="Baek, Yun Hee" sort="Baek, Yun Hee" uniqKey="Baek Y" first="Yun Hee" last="Baek">Yun Hee Baek</name><name sortKey="Choi, Young Ki" sort="Choi, Young Ki" uniqKey="Choi Y" first="Young-Ki" last="Choi">Young-Ki Choi</name><name sortKey="Kim, Yang Hoon" sort="Kim, Yang Hoon" uniqKey="Kim Y" first="Yang-Hoon" last="Kim">Yang-Hoon Kim</name><name sortKey="Song, Min Suk" sort="Song, Min Suk" uniqKey="Song M" first="Min-Suk" last="Song">Min-Suk Song</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F57 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000F57 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:28119682
|texte= One-Pot Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) for Detecting MERS-CoV.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:28119682" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |