MersV1, PubMed, Checkpoint, bibRecord, 000C77

Human intestinal tract serves as an alternative infection route for Middle East respiratory syndrome coronavirus.

Identifieur interne : 000C77 ( PubMed/Checkpoint ); précédent : 000C76; suivant : 000C78

Human intestinal tract serves as an alternative infection route for Middle East respiratory syndrome coronavirus.

Source :

Science advances [ 2375-2548 ] ; 2017.

RBID : pubmed:29152574

Descripteurs français

KwdFr :
- ARN viral (génétique), ARN viral (métabolisme), Animaux, Cellules Caco-2, Cellules épithéliales (cytologie), Cellules épithéliales (virologie), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité), Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie), Dipeptidyl peptidase 4 (génétique), Dipeptidyl peptidase 4 (métabolisme), Femelle, Fèces (virologie), Humains, Infections à coronavirus (anatomopathologie), Infections à coronavirus (traitement médicamenteux), Infections à coronavirus (virologie), Intestin grêle (anatomopathologie), Intestin grêle (cytologie), Intestin grêle (virologie), Poumon (anatomopathologie), Poumon (virologie), Souris, Souris transgéniques.
MESH :
- anatomopathologie : Infections à coronavirus, Intestin grêle, Poumon.
- cytologie : Cellules épithéliales, Intestin grêle.
- génétique : ARN viral, Coronavirus du syndrome respiratoire du Moyen-Orient, Dipeptidyl peptidase 4.
- métabolisme : ARN viral, Dipeptidyl peptidase 4.
- pathogénicité : Coronavirus du syndrome respiratoire du Moyen-Orient.
- physiologie : Coronavirus du syndrome respiratoire du Moyen-Orient.
- traitement médicamenteux : Infections à coronavirus.
- virologie : Cellules épithéliales, Fèces, Infections à coronavirus, Intestin grêle, Poumon.
- Animaux, Cellules Caco-2, Femelle, Humains, Souris, Souris transgéniques.

English descriptors

KwdEn :
- Animals, Caco-2 Cells, Coronavirus Infections (drug therapy), Coronavirus Infections (pathology), Coronavirus Infections (virology), Dipeptidyl Peptidase 4 (genetics), Dipeptidyl Peptidase 4 (metabolism), Epithelial Cells (cytology), Epithelial Cells (virology), Feces (virology), Female, Humans, Intestine, Small (cytology), Intestine, Small (pathology), Intestine, Small (virology), Lung (pathology), Lung (virology), Mice, Mice, Transgenic, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (pathogenicity), Middle East Respiratory Syndrome Coronavirus (physiology), Pantoprazole (therapeutic use), RNA, Viral (genetics), RNA, Viral (metabolism).
MESH :
- chemical , genetics : Dipeptidyl Peptidase 4, RNA, Viral.
- cytology : Epithelial Cells, Intestine, Small.
- drug therapy : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- chemical , metabolism : Dipeptidyl Peptidase 4, RNA, Viral.
- pathogenicity : Middle East Respiratory Syndrome Coronavirus.
- pathology : Coronavirus Infections, Intestine, Small, Lung.
- physiology : Middle East Respiratory Syndrome Coronavirus.
- chemical , therapeutic use : Pantoprazole.
- virology : Coronavirus Infections, Epithelial Cells, Feces, Intestine, Small, Lung.
- Animals, Caco-2 Cells, Female, Humans, Mice, Mice, Transgenic.

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) has caused human respiratory infections with a high case fatality rate since 2012. However, the mode of virus transmission is not well understood. The findings of epidemiological and virological studies prompted us to hypothesize that the human gastrointestinal tract could serve as an alternative route to acquire MERS-CoV infection. We demonstrated that human primary intestinal epithelial cells, small intestine explants, and intestinal organoids were highly susceptible to MERS-CoV and can sustain robust viral replication. We also identified the evidence of enteric MERS-CoV infection in the stool specimen of a clinical patient. MERS-CoV was considerably resistant to fed-state gastrointestinal fluids but less tolerant to highly acidic fasted-state gastric fluid. In polarized Caco-2 cells cultured in Transwell inserts, apical MERS-CoV inoculation was more effective in establishing infection than basolateral inoculation. Notably, direct intragastric inoculation of MERS-CoV caused a lethal infection in human DPP4 transgenic mice. Histological examination revealed MERS-CoV enteric infection in all inoculated mice, as shown by the presence of virus-positive cells, progressive inflammation, and epithelial degeneration in small intestines, which were exaggerated in the mice pretreated with the proton pump inhibitor pantoprazole. With the progression of the enteric infection, inflammation, virus-positive cells, and live viruses emerged in the lung tissues, indicating the development of sequential respiratory infection. Taken together, these data suggest that the human intestinal tract may serve as an alternative infection route for MERS-CoV.

DOI: 10.1126/sciadv.aao4966
PubMed: 29152574

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 000A85
to stream PubMed, to step Curation: 000A85

Links to Exploration step

pubmed:29152574

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Human intestinal tract serves as an alternative infection route for Middle East respiratory syndrome coronavirus.</title>
<author><name sortKey="Zhou, Jie" sort="Zhou, Jie" uniqKey="Zhou J" first="Jie" last="Zhou">Jie Zhou</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Cun" sort="Li, Cun" uniqKey="Li C" first="Cun" last="Li">Cun Li</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name>
<affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chu, Hin" sort="Chu, Hin" uniqKey="Chu H" first="Hin" last="Chu">Hin Chu</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Dong" sort="Wang, Dong" uniqKey="Wang D" first="Dong" last="Wang">Dong Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yan, Helen Hoi Ning" sort="Yan, Helen Hoi Ning" uniqKey="Yan H" first="Helen Hoi-Ning" last="Yan">Helen Hoi-Ning Yan</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Poon, Vincent Kwok Man" sort="Poon, Vincent Kwok Man" uniqKey="Poon V" first="Vincent Kwok-Man" last="Poon">Vincent Kwok-Man Poon</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wen, Lei" sort="Wen, Lei" uniqKey="Wen L" first="Lei" last="Wen">Lei Wen</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wong, Bosco Ho Yin" sort="Wong, Bosco Ho Yin" uniqKey="Wong B" first="Bosco Ho-Yin" last="Wong">Bosco Ho-Yin Wong</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhao, Xiaoyu" sort="Zhao, Xiaoyu" uniqKey="Zhao X" first="Xiaoyu" last="Zhao">Xiaoyu Zhao</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chiu, Man Chun" sort="Chiu, Man Chun" uniqKey="Chiu M" first="Man Chun" last="Chiu">Man Chun Chiu</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yang, Dong" sort="Yang, Dong" uniqKey="Yang D" first="Dong" last="Yang">Dong Yang</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Yixin" sort="Wang, Yixin" uniqKey="Wang Y" first="Yixin" last="Wang">Yixin Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Au Yeung, Rex K H" sort="Au Yeung, Rex K H" uniqKey="Au Yeung R" first="Rex K H" last="Au-Yeung">Rex K H. Au-Yeung</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chan, Ivy Hau Yee" sort="Chan, Ivy Hau Yee" uniqKey="Chan I" first="Ivy Hau-Yee" last="Chan">Ivy Hau-Yee Chan</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Surgery, Queen Mary Hospital, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Surgery, Queen Mary Hospital, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sun, Shihui" sort="Sun, Shihui" uniqKey="Sun S" first="Shihui" last="Sun">Shihui Sun</name>
<affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chan, Jasper Fuk Woo" sort="Chan, Jasper Fuk Woo" uniqKey="Chan J" first="Jasper Fuk-Woo" last="Chan">Jasper Fuk-Woo Chan</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Memish, Ziad A" sort="Memish, Ziad A" uniqKey="Memish Z" first="Ziad A" last="Memish">Ziad A. Memish</name>
<affiliation><nlm:affiliation>Ministry of Health and College of Medicine, Alfaisal University, Riyadh, Kingdom of Saudi Arabia.</nlm:affiliation>
<wicri:noCountry code="subField">Kingdom of Saudi Arabia</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Corman, Victor M" sort="Corman, Victor M" uniqKey="Corman V" first="Victor M" last="Corman">Victor M. Corman</name>
<affiliation wicri:level="3"><nlm:affiliation>Institute of Virology, Charité-Universitätsmedizin Berlin, Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Virology, Charité-Universitätsmedizin Berlin, Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name>
<affiliation wicri:level="3"><nlm:affiliation>Institute of Virology, Charité-Universitätsmedizin Berlin, Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Virology, Charité-Universitätsmedizin Berlin, Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hung, Ivan Fan Ngai" sort="Hung, Ivan Fan Ngai" uniqKey="Hung I" first="Ivan Fan-Ngai" last="Hung">Ivan Fan-Ngai Hung</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medicine, University of Hong Kong, Queen Mary Hospital, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Medicine, University of Hong Kong, Queen Mary Hospital, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name>
<affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Leung, Suet Yi" sort="Leung, Suet Yi" uniqKey="Leung S" first="Suet Yi" last="Leung">Suet Yi Leung</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2017">2017</date>
<idno type="RBID">pubmed:29152574</idno>
<idno type="pmid">29152574</idno>
<idno type="doi">10.1126/sciadv.aao4966</idno>
<idno type="wicri:Area/PubMed/Corpus">000A85</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000A85</idno>
<idno type="wicri:Area/PubMed/Curation">000A85</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000A85</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000C77</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000C77</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Human intestinal tract serves as an alternative infection route for Middle East respiratory syndrome coronavirus.</title>
<author><name sortKey="Zhou, Jie" sort="Zhou, Jie" uniqKey="Zhou J" first="Jie" last="Zhou">Jie Zhou</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Cun" sort="Li, Cun" uniqKey="Li C" first="Cun" last="Li">Cun Li</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name>
<affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chu, Hin" sort="Chu, Hin" uniqKey="Chu H" first="Hin" last="Chu">Hin Chu</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Dong" sort="Wang, Dong" uniqKey="Wang D" first="Dong" last="Wang">Dong Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yan, Helen Hoi Ning" sort="Yan, Helen Hoi Ning" uniqKey="Yan H" first="Helen Hoi-Ning" last="Yan">Helen Hoi-Ning Yan</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Poon, Vincent Kwok Man" sort="Poon, Vincent Kwok Man" uniqKey="Poon V" first="Vincent Kwok-Man" last="Poon">Vincent Kwok-Man Poon</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wen, Lei" sort="Wen, Lei" uniqKey="Wen L" first="Lei" last="Wen">Lei Wen</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wong, Bosco Ho Yin" sort="Wong, Bosco Ho Yin" uniqKey="Wong B" first="Bosco Ho-Yin" last="Wong">Bosco Ho-Yin Wong</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhao, Xiaoyu" sort="Zhao, Xiaoyu" uniqKey="Zhao X" first="Xiaoyu" last="Zhao">Xiaoyu Zhao</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chiu, Man Chun" sort="Chiu, Man Chun" uniqKey="Chiu M" first="Man Chun" last="Chiu">Man Chun Chiu</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yang, Dong" sort="Yang, Dong" uniqKey="Yang D" first="Dong" last="Yang">Dong Yang</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Yixin" sort="Wang, Yixin" uniqKey="Wang Y" first="Yixin" last="Wang">Yixin Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Au Yeung, Rex K H" sort="Au Yeung, Rex K H" uniqKey="Au Yeung R" first="Rex K H" last="Au-Yeung">Rex K H. Au-Yeung</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chan, Ivy Hau Yee" sort="Chan, Ivy Hau Yee" uniqKey="Chan I" first="Ivy Hau-Yee" last="Chan">Ivy Hau-Yee Chan</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Surgery, Queen Mary Hospital, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Surgery, Queen Mary Hospital, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sun, Shihui" sort="Sun, Shihui" uniqKey="Sun S" first="Shihui" last="Sun">Shihui Sun</name>
<affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chan, Jasper Fuk Woo" sort="Chan, Jasper Fuk Woo" uniqKey="Chan J" first="Jasper Fuk-Woo" last="Chan">Jasper Fuk-Woo Chan</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Memish, Ziad A" sort="Memish, Ziad A" uniqKey="Memish Z" first="Ziad A" last="Memish">Ziad A. Memish</name>
<affiliation><nlm:affiliation>Ministry of Health and College of Medicine, Alfaisal University, Riyadh, Kingdom of Saudi Arabia.</nlm:affiliation>
<wicri:noCountry code="subField">Kingdom of Saudi Arabia</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Corman, Victor M" sort="Corman, Victor M" uniqKey="Corman V" first="Victor M" last="Corman">Victor M. Corman</name>
<affiliation wicri:level="3"><nlm:affiliation>Institute of Virology, Charité-Universitätsmedizin Berlin, Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Virology, Charité-Universitätsmedizin Berlin, Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name>
<affiliation wicri:level="3"><nlm:affiliation>Institute of Virology, Charité-Universitätsmedizin Berlin, Berlin, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Virology, Charité-Universitätsmedizin Berlin, Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hung, Ivan Fan Ngai" sort="Hung, Ivan Fan Ngai" uniqKey="Hung I" first="Ivan Fan-Ngai" last="Hung">Ivan Fan-Ngai Hung</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medicine, University of Hong Kong, Queen Mary Hospital, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Medicine, University of Hong Kong, Queen Mary Hospital, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name>
<affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Leung, Suet Yi" sort="Leung, Suet Yi" uniqKey="Leung S" first="Suet Yi" last="Leung">Suet Yi Leung</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong</wicri:regionArea>
<wicri:noRegion>Hong Kong</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Science advances</title>
<idno type="eISSN">2375-2548</idno>
<imprint><date when="2017" type="published">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Caco-2 Cells</term>
<term>Coronavirus Infections (drug therapy)</term>
<term>Coronavirus Infections (pathology)</term>
<term>Coronavirus Infections (virology)</term>
<term>Dipeptidyl Peptidase 4 (genetics)</term>
<term>Dipeptidyl Peptidase 4 (metabolism)</term>
<term>Epithelial Cells (cytology)</term>
<term>Epithelial Cells (virology)</term>
<term>Feces (virology)</term>
<term>Female</term>
<term>Humans</term>
<term>Intestine, Small (cytology)</term>
<term>Intestine, Small (pathology)</term>
<term>Intestine, Small (virology)</term>
<term>Lung (pathology)</term>
<term>Lung (virology)</term>
<term>Mice</term>
<term>Mice, Transgenic</term>
<term>Middle East Respiratory Syndrome Coronavirus (genetics)</term>
<term>Middle East Respiratory Syndrome Coronavirus (pathogenicity)</term>
<term>Middle East Respiratory Syndrome Coronavirus (physiology)</term>
<term>Pantoprazole (therapeutic use)</term>
<term>RNA, Viral (genetics)</term>
<term>RNA, Viral (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term>
<term>ARN viral (métabolisme)</term>
<term>Animaux</term>
<term>Cellules Caco-2</term>
<term>Cellules épithéliales (cytologie)</term>
<term>Cellules épithéliales (virologie)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie)</term>
<term>Dipeptidyl peptidase 4 (génétique)</term>
<term>Dipeptidyl peptidase 4 (métabolisme)</term>
<term>Femelle</term>
<term>Fèces (virologie)</term>
<term>Humains</term>
<term>Infections à coronavirus (anatomopathologie)</term>
<term>Infections à coronavirus (traitement médicamenteux)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Intestin grêle (anatomopathologie)</term>
<term>Intestin grêle (cytologie)</term>
<term>Intestin grêle (virologie)</term>
<term>Poumon (anatomopathologie)</term>
<term>Poumon (virologie)</term>
<term>Souris</term>
<term>Souris transgéniques</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Dipeptidyl Peptidase 4</term>
<term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Intestin grêle</term>
<term>Poumon</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules épithéliales</term>
<term>Intestin grêle</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Epithelial Cells</term>
<term>Intestine, Small</term>
</keywords>
<keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Dipeptidyl peptidase 4</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Dipeptidyl Peptidase 4</term>
<term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term>
<term>Dipeptidyl peptidase 4</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Intestine, Small</term>
<term>Lung</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Pantoprazole</term>
</keywords>
<keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term>
<term>Fèces</term>
<term>Infections à coronavirus</term>
<term>Intestin grêle</term>
<term>Poumon</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Epithelial Cells</term>
<term>Feces</term>
<term>Intestine, Small</term>
<term>Lung</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Caco-2 Cells</term>
<term>Female</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Transgenic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules Caco-2</term>
<term>Femelle</term>
<term>Humains</term>
<term>Souris</term>
<term>Souris transgéniques</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) has caused human respiratory infections with a high case fatality rate since 2012. However, the mode of virus transmission is not well understood. The findings of epidemiological and virological studies prompted us to hypothesize that the human gastrointestinal tract could serve as an alternative route to acquire MERS-CoV infection. We demonstrated that human primary intestinal epithelial cells, small intestine explants, and intestinal organoids were highly susceptible to MERS-CoV and can sustain robust viral replication. We also identified the evidence of enteric MERS-CoV infection in the stool specimen of a clinical patient. MERS-CoV was considerably resistant to fed-state gastrointestinal fluids but less tolerant to highly acidic fasted-state gastric fluid. In polarized Caco-2 cells cultured in Transwell inserts, apical MERS-CoV inoculation was more effective in establishing infection than basolateral inoculation. Notably, direct intragastric inoculation of MERS-CoV caused a lethal infection in human DPP4 transgenic mice. Histological examination revealed MERS-CoV enteric infection in all inoculated mice, as shown by the presence of virus-positive cells, progressive inflammation, and epithelial degeneration in small intestines, which were exaggerated in the mice pretreated with the proton pump inhibitor pantoprazole. With the progression of the enteric infection, inflammation, virus-positive cells, and live viruses emerged in the lung tissues, indicating the development of sequential respiratory infection. Taken together, these data suggest that the human intestinal tract may serve as an alternative infection route for MERS-CoV.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29152574</PMID>
<DateCompleted><Year>2019</Year>
<Month>06</Month>
<Day>27</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>06</Month>
<Day>27</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">2375-2548</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>3</Volume>
<Issue>11</Issue>
<PubDate><Year>2017</Year>
<Month>11</Month>
</PubDate>
</JournalIssue>
<Title>Science advances</Title>
<ISOAbbreviation>Sci Adv</ISOAbbreviation>
</Journal>
<ArticleTitle>Human intestinal tract serves as an alternative infection route for Middle East respiratory syndrome coronavirus.</ArticleTitle>
<Pagination><MedlinePgn>eaao4966</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1126/sciadv.aao4966</ELocationID>
<Abstract><AbstractText>Middle East respiratory syndrome coronavirus (MERS-CoV) has caused human respiratory infections with a high case fatality rate since 2012. However, the mode of virus transmission is not well understood. The findings of epidemiological and virological studies prompted us to hypothesize that the human gastrointestinal tract could serve as an alternative route to acquire MERS-CoV infection. We demonstrated that human primary intestinal epithelial cells, small intestine explants, and intestinal organoids were highly susceptible to MERS-CoV and can sustain robust viral replication. We also identified the evidence of enteric MERS-CoV infection in the stool specimen of a clinical patient. MERS-CoV was considerably resistant to fed-state gastrointestinal fluids but less tolerant to highly acidic fasted-state gastric fluid. In polarized Caco-2 cells cultured in Transwell inserts, apical MERS-CoV inoculation was more effective in establishing infection than basolateral inoculation. Notably, direct intragastric inoculation of MERS-CoV caused a lethal infection in human DPP4 transgenic mice. Histological examination revealed MERS-CoV enteric infection in all inoculated mice, as shown by the presence of virus-positive cells, progressive inflammation, and epithelial degeneration in small intestines, which were exaggerated in the mice pretreated with the proton pump inhibitor pantoprazole. With the progression of the enteric infection, inflammation, virus-positive cells, and live viruses emerged in the lung tissues, indicating the development of sequential respiratory infection. Taken together, these data suggest that the human intestinal tract may serve as an alternative infection route for MERS-CoV.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhou</LastName>
<ForeName>Jie</ForeName>
<Initials>J</Initials>
<Identifier Source="ORCID">0000-0002-2948-3873</Identifier>
<AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Research Centre of Infection and Immunology, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Cun</ForeName>
<Initials>C</Initials>
<Identifier Source="ORCID">0000-0003-3003-0000</Identifier>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhao</LastName>
<ForeName>Guangyu</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chu</LastName>
<ForeName>Hin</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Research Centre of Infection and Immunology, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Dong</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yan</LastName>
<ForeName>Helen Hoi-Ning</ForeName>
<Initials>HH</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Poon</LastName>
<ForeName>Vincent Kwok-Man</ForeName>
<Initials>VK</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wen</LastName>
<ForeName>Lei</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wong</LastName>
<ForeName>Bosco Ho-Yin</ForeName>
<Initials>BH</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhao</LastName>
<ForeName>Xiaoyu</ForeName>
<Initials>X</Initials>
<Identifier Source="ORCID">0000-0002-0347-9104</Identifier>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chiu</LastName>
<ForeName>Man Chun</ForeName>
<Initials>MC</Initials>
<Identifier Source="ORCID">0000-0001-9503-6396</Identifier>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Dong</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Yixin</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Au-Yeung</LastName>
<ForeName>Rex K H</ForeName>
<Initials>RKH</Initials>
<Identifier Source="ORCID">0000-0002-3137-0886</Identifier>
<AffiliationInfo><Affiliation>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chan</LastName>
<ForeName>Ivy Hau-Yee</ForeName>
<Initials>IH</Initials>
<AffiliationInfo><Affiliation>Department of Surgery, Queen Mary Hospital, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sun</LastName>
<ForeName>Shihui</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chan</LastName>
<ForeName>Jasper Fuk-Woo</ForeName>
<Initials>JF</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Research Centre of Infection and Immunology, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Carol Yu Centre for Infection, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>To</LastName>
<ForeName>Kelvin Kai-Wang</ForeName>
<Initials>KK</Initials>
<Identifier Source="ORCID">0000-0002-1921-5824</Identifier>
<AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Research Centre of Infection and Immunology, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Carol Yu Centre for Infection, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Memish</LastName>
<ForeName>Ziad A</ForeName>
<Initials>ZA</Initials>
<AffiliationInfo><Affiliation>Ministry of Health and College of Medicine, Alfaisal University, Riyadh, Kingdom of Saudi Arabia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Hubert Department of Global Health, Rollins School of Public Health, Emory University, Atlanta, GA 30322, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Corman</LastName>
<ForeName>Victor M</ForeName>
<Initials>VM</Initials>
<Identifier Source="ORCID">0000-0002-3605-0136</Identifier>
<AffiliationInfo><Affiliation>Institute of Virology, Charité-Universitätsmedizin Berlin, Berlin, Germany.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>German Centre for Infection Research (DZIF), Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Drosten</LastName>
<ForeName>Christian</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Institute of Virology, Charité-Universitätsmedizin Berlin, Berlin, Germany.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>German Centre for Infection Research (DZIF), Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hung</LastName>
<ForeName>Ivan Fan-Ngai</ForeName>
<Initials>IF</Initials>
<AffiliationInfo><Affiliation>Department of Medicine, University of Hong Kong, Queen Mary Hospital, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhou</LastName>
<ForeName>Yusen</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Leung</LastName>
<ForeName>Suet Yi</ForeName>
<Initials>SY</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yuen</LastName>
<ForeName>Kwok-Yung</ForeName>
<Initials>KY</Initials>
<Identifier Source="ORCID">0000-0002-2948-3873</Identifier>
<AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Research Centre of Infection and Immunology, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Carol Yu Centre for Infection, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2017</Year>
<Month>11</Month>
<Day>15</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Sci Adv</MedlineTA>
<NlmUniqueID>101653440</NlmUniqueID>
<ISSNLinking>2375-2548</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>D8TST4O562</RegistryNumber>
<NameOfSubstance UI="D000077402">Pantoprazole</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.14.5</RegistryNumber>
<NameOfSubstance UI="C042807">DPP4 protein, human</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.14.5</RegistryNumber>
<NameOfSubstance UI="D018819">Dipeptidyl Peptidase 4</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018938" MajorTopicYN="N">Caco-2 Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName>
<QualifierName UI="Q000188" MajorTopicYN="N">drug therapy</QualifierName>
<QualifierName UI="Q000473" MajorTopicYN="Y">pathology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018819" MajorTopicYN="N">Dipeptidyl Peptidase 4</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004847" MajorTopicYN="N">Epithelial Cells</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005243" MajorTopicYN="N">Feces</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007421" MajorTopicYN="N">Intestine, Small</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008168" MajorTopicYN="N">Lung</DescriptorName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008822" MajorTopicYN="N">Mice, Transgenic</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000077402" MajorTopicYN="N">Pantoprazole</DescriptorName>
<QualifierName UI="Q000627" MajorTopicYN="N">therapeutic use</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year>
<Month>07</Month>
<Day>27</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2017</Year>
<Month>10</Month>
<Day>20</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>11</Month>
<Day>21</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>11</Month>
<Day>21</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2019</Year>
<Month>6</Month>
<Day>30</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">29152574</ArticleId>
<ArticleId IdType="doi">10.1126/sciadv.aao4966</ArticleId>
<ArticleId IdType="pii">aao4966</ArticleId>
<ArticleId IdType="pmc">PMC5687858</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Hepatology. 2006 Feb;43(2 Suppl 1):S164-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16447259</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Infect Dis. 2003 Apr 15;36(8):985-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12684910</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2014 Dec;20(12 ):1999-2005</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25418529</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2016 Mar 1;213(5):712-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26486634</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Infect Drug Resist. 2014 Nov 03;7:281-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25395865</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Pathol. 2004 Jun;203(2):622-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15141376</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2012 Nov 8;367 (19):1814-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2014 May 1;209(9):1331-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24065148</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Curr. 2013 Nov 13;5:null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24270586</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2014 Jun;20(6):1012-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24857749</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2013 Jun 27;368(26):2487-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23718156</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):9081-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27457935</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2014 Jun 12;19(23 ):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24957745</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 1985 Dec;66 ( Pt 12):2725-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2999315</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2015 Dec 23;10(12):e0145561</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26701103</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2012 Jul 03;3(4):e00159-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22761392</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2016 Mar 15;213(6):904-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26203058</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Exp Mol Med. 2015 Aug 28;47:e181</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26315600</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Pharmacol. 2013 Jul 08;4:79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23847534</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2013 Jun 13;18(24):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23787161</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gastroenterology. 2011 Nov;141(5):1762-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21889923</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Genet. 2014 Jun;46(6):573-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24816253</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virol J. 2015 Dec 22;12 :218</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26690369</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gastroenterology. 2003 Oct;125(4):1011-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14517783</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hepatology. 2011 Jul;54(1):109-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21503941</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Infect Dis. 2016 Mar 15;62(6):755-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26679623</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Microbiol Rev. 2015 Apr;28(2):465-522</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25810418</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mucosal Immunol. 2009 Mar;2(2):100-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19129753</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2013 Aug 1;369(5):407-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23782161</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet Infect Dis. 2014 Nov;14 (11):1113-1122</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25189350</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2004 Sep;10(9):1550-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15498155</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antiviral Res. 2015 Nov;123:120-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26408355</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vet Clin North Am Food Anim Pract. 2010 Jul;26(2):349-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20619189</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2014 Jul;20(7):1263-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24960335</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Jun;87(12):6604-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23552422</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2015 Oct 07;90(1):57-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26446606</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet Infect Dis. 2013 Sep;13(9):752-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23891402</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Infect Dis. 2015 Sep;38:65-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26216766</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 May;87(9):5300-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23449793</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2011 May;85(10):4673-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21411541</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2013 Sep 19;18(38):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24084338</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Pathog. 2012;2012:738590</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23097708</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Infect Dis. 2016 Feb 15;62(4):477-483</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26565003</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Allemagne</li>
<li>République populaire de Chine</li>
</country>
<region><li>Berlin</li>
</region>
<settlement><li>Berlin</li>
<li>Pékin</li>
</settlement>
</list>
<tree><noCountry><name sortKey="Memish, Ziad A" sort="Memish, Ziad A" uniqKey="Memish Z" first="Ziad A" last="Memish">Ziad A. Memish</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Zhou, Jie" sort="Zhou, Jie" uniqKey="Zhou J" first="Jie" last="Zhou">Jie Zhou</name>
</noRegion>
<name sortKey="Au Yeung, Rex K H" sort="Au Yeung, Rex K H" uniqKey="Au Yeung R" first="Rex K H" last="Au-Yeung">Rex K H. Au-Yeung</name>
<name sortKey="Chan, Ivy Hau Yee" sort="Chan, Ivy Hau Yee" uniqKey="Chan I" first="Ivy Hau-Yee" last="Chan">Ivy Hau-Yee Chan</name>
<name sortKey="Chan, Jasper Fuk Woo" sort="Chan, Jasper Fuk Woo" uniqKey="Chan J" first="Jasper Fuk-Woo" last="Chan">Jasper Fuk-Woo Chan</name>
<name sortKey="Chiu, Man Chun" sort="Chiu, Man Chun" uniqKey="Chiu M" first="Man Chun" last="Chiu">Man Chun Chiu</name>
<name sortKey="Chu, Hin" sort="Chu, Hin" uniqKey="Chu H" first="Hin" last="Chu">Hin Chu</name>
<name sortKey="Hung, Ivan Fan Ngai" sort="Hung, Ivan Fan Ngai" uniqKey="Hung I" first="Ivan Fan-Ngai" last="Hung">Ivan Fan-Ngai Hung</name>
<name sortKey="Leung, Suet Yi" sort="Leung, Suet Yi" uniqKey="Leung S" first="Suet Yi" last="Leung">Suet Yi Leung</name>
<name sortKey="Li, Cun" sort="Li, Cun" uniqKey="Li C" first="Cun" last="Li">Cun Li</name>
<name sortKey="Poon, Vincent Kwok Man" sort="Poon, Vincent Kwok Man" uniqKey="Poon V" first="Vincent Kwok-Man" last="Poon">Vincent Kwok-Man Poon</name>
<name sortKey="Sun, Shihui" sort="Sun, Shihui" uniqKey="Sun S" first="Shihui" last="Sun">Shihui Sun</name>
<name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name>
<name sortKey="Wang, Dong" sort="Wang, Dong" uniqKey="Wang D" first="Dong" last="Wang">Dong Wang</name>
<name sortKey="Wang, Yixin" sort="Wang, Yixin" uniqKey="Wang Y" first="Yixin" last="Wang">Yixin Wang</name>
<name sortKey="Wen, Lei" sort="Wen, Lei" uniqKey="Wen L" first="Lei" last="Wen">Lei Wen</name>
<name sortKey="Wong, Bosco Ho Yin" sort="Wong, Bosco Ho Yin" uniqKey="Wong B" first="Bosco Ho-Yin" last="Wong">Bosco Ho-Yin Wong</name>
<name sortKey="Yan, Helen Hoi Ning" sort="Yan, Helen Hoi Ning" uniqKey="Yan H" first="Helen Hoi-Ning" last="Yan">Helen Hoi-Ning Yan</name>
<name sortKey="Yang, Dong" sort="Yang, Dong" uniqKey="Yang D" first="Dong" last="Yang">Dong Yang</name>
<name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name>
<name sortKey="Zhao, Xiaoyu" sort="Zhao, Xiaoyu" uniqKey="Zhao X" first="Xiaoyu" last="Zhao">Xiaoyu Zhao</name>
<name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name>
</country>
<country name="Allemagne"><region name="Berlin"><name sortKey="Corman, Victor M" sort="Corman, Victor M" uniqKey="Corman V" first="Victor M" last="Corman">Victor M. Corman</name>
</region>
<name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C77 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000C77 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    PubMed
   |étape=   Checkpoint
   |type=    RBID
   |clé=     pubmed:29152574
   |texte=   Human intestinal tract serves as an alternative infection route for Middle East respiratory syndrome coronavirus.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i   -Sk "pubmed:29152574" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

Human intestinal tract serves as an alternative infection route for Middle East respiratory syndrome coronavirus.

Human intestinal tract serves as an alternative infection route for Middle East respiratory syndrome coronavirus.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki