Performance Evaluation of the PowerChek MERS (upE & ORF1a) Real-Time PCR Kit for the Detection of Middle East Respiratory Syndrome Coronavirus RNA.
Identifieur interne : 000B74 ( PubMed/Checkpoint ); précédent : 000B73; suivant : 000B75Performance Evaluation of the PowerChek MERS (upE & ORF1a) Real-Time PCR Kit for the Detection of Middle East Respiratory Syndrome Coronavirus RNA.
Auteurs : Hee Jae Huh [Corée du Sud] ; Ji Youn Kim [Corée du Sud] ; Hyeon Jeong Kwon [Corée du Sud] ; Sun Ae Yun [Corée du Sud] ; Myoung Keun Lee [Corée du Sud] ; Chang Seok Ki [Corée du Sud] ; Nam Yong Lee [Corée du Sud] ; Jong Won Kim [Corée du Sud]Source :
- Annals of laboratory medicine [ 2234-3814 ] ; 2017.
Descripteurs français
- KwdFr :
- ARN viral (analyse), ARN viral (métabolisme), Analyse de séquence d'ARN, Cadres ouverts de lecture (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Expectoration (virologie), Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Partie nasale du pharynx (virologie), Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Trousses de réactifs pour diagnostic.
- MESH :
- analyse : ARN viral.
- diagnostic : Infections à coronavirus.
- génétique : Cadres ouverts de lecture, Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- métabolisme : ARN viral.
- virologie : Expectoration, Infections à coronavirus, Partie nasale du pharynx.
- Analyse de séquence d'ARN, Humains, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Trousses de réactifs pour diagnostic.
English descriptors
- KwdEn :
- Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Nasopharynx (virology), Open Reading Frames (genetics), RNA, Viral (analysis), RNA, Viral (metabolism), Reagent Kits, Diagnostic, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Analysis, RNA, Sputum (virology).
- MESH :
- chemical , analysis : RNA, Viral.
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus, Open Reading Frames.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- chemical , metabolism : RNA, Viral.
- virology : Coronavirus Infections, Nasopharynx, Sputum.
- Humans, Reagent Kits, Diagnostic, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Analysis, RNA.
Abstract
Molecular detection of Middle East respiratory syndrome coronavirus (MERS-CoV) using real-time reverse transcription (rRT)-PCR assays is the method of choice for diagnosis of MERS. We evaluated the performance of the PowerChek MERS (upE & ORF1a) real-time PCR Kit (PowerChek MERS assay; Kogene Biotech, Korea) a one-step rRT-PCR assay for the qualitative detection of MERS-CoV.
DOI: 10.3343/alm.2017.37.6.494
PubMed: 28840986
Affiliations:
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pubmed:28840986Le document en format XML
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<front><div type="abstract" xml:lang="en">Molecular detection of Middle East respiratory syndrome coronavirus (MERS-CoV) using real-time reverse transcription (rRT)-PCR assays is the method of choice for diagnosis of MERS. We evaluated the performance of the PowerChek MERS (upE & ORF1a) real-time PCR Kit (PowerChek MERS assay; Kogene Biotech, Korea) a one-step rRT-PCR assay for the qualitative detection of MERS-CoV.</div>
</front>
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<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28840986</PMID>
<DateCompleted><Year>2018</Year>
<Month>05</Month>
<Day>11</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Electronic">2234-3814</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>37</Volume>
<Issue>6</Issue>
<PubDate><Year>2017</Year>
<Month>Nov</Month>
</PubDate>
</JournalIssue>
<Title>Annals of laboratory medicine</Title>
<ISOAbbreviation>Ann Lab Med</ISOAbbreviation>
</Journal>
<ArticleTitle>Performance Evaluation of the PowerChek MERS (upE & ORF1a) Real-Time PCR Kit for the Detection of Middle East Respiratory Syndrome Coronavirus RNA.</ArticleTitle>
<Pagination><MedlinePgn>494-498</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.3343/alm.2017.37.6.494</ELocationID>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Molecular detection of Middle East respiratory syndrome coronavirus (MERS-CoV) using real-time reverse transcription (rRT)-PCR assays is the method of choice for diagnosis of MERS. We evaluated the performance of the PowerChek MERS (upE & ORF1a) real-time PCR Kit (PowerChek MERS assay; Kogene Biotech, Korea) a one-step rRT-PCR assay for the qualitative detection of MERS-CoV.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">We evaluated PowerChek MERS assay performance in comparison with nested RT-PCR and sequencing of the RNA-dependent RNA polymerase (RdRp) and N genes. To evaluate diagnostic sensitivity and specificity, 100 clinical specimens (50 positive and 50 negative for MERS-CoV) were simultaneously tested by using the PowerChek MERS and sequencing assays. Assay performance, including limit of detection and precision, was evaluated in vitro by using MERS-CoV RNA transcripts. Analytical specificity was evaluated with a diverse collection of 16 respiratory virus-positive clinical specimens and 14 respiratory bacterial isolates.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">The 95% limits of detection of the PowerChek MERS assay for the upE and the open rading frame (ORF)1a were 16.2 copies/μL and 8.2 copies/μL, respectively. No cross-reactivity was observed. The diagnostic sensitivity and specificity of the PowerChek MERS assay were both 100% (95% confidence interval, 91.1-100%).</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The PowerChek MERS assay is a straightforward and accurate assay for detecting MERS-CoV RNA. The assay will be a useful tool for the rapid diagnosis of MERS and could prove especially important for MERS outbreak control.</AbstractText>
<CopyrightInformation>© The Korean Society for Laboratory Medicine</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Huh</LastName>
<ForeName>Hee Jae</ForeName>
<Initials>HJ</Initials>
<AffiliationInfo><Affiliation>Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Ji Youn</ForeName>
<Initials>JY</Initials>
<AffiliationInfo><Affiliation>Center for Clinical Medicine, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kwon</LastName>
<ForeName>Hyeon Jeong</ForeName>
<Initials>HJ</Initials>
<AffiliationInfo><Affiliation>Center for Clinical Medicine, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yun</LastName>
<ForeName>Sun Ae</ForeName>
<Initials>SA</Initials>
<AffiliationInfo><Affiliation>Center for Clinical Medicine, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lee</LastName>
<ForeName>Myoung Keun</ForeName>
<Initials>MK</Initials>
<AffiliationInfo><Affiliation>Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ki</LastName>
<ForeName>Chang Seok</ForeName>
<Initials>CS</Initials>
<AffiliationInfo><Affiliation>Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. changski@skku.edu.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lee</LastName>
<ForeName>Nam Yong</ForeName>
<Initials>NY</Initials>
<AffiliationInfo><Affiliation>Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Jong Won</ForeName>
<Initials>JW</Initials>
<AffiliationInfo><Affiliation>Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
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<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType>
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<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009305" MajorTopicYN="N">Nasopharynx</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016366" MajorTopicYN="N">Open Reading Frames</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011933" MajorTopicYN="N">Reagent Kits, Diagnostic</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017423" MajorTopicYN="N">Sequence Analysis, RNA</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013183" MajorTopicYN="N">Sputum</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">MERS-CoV</Keyword>
<Keyword MajorTopicYN="N">Performance</Keyword>
<Keyword MajorTopicYN="N">Real-time reverse-transcription PCR</Keyword>
</KeywordList>
<CoiStatement>No potential conflicts of interest relevant to this article were reported.</CoiStatement>
</MedlineCitation>
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<Month>03</Month>
<Day>26</Day>
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<PubMedPubDate PubStatus="accepted"><Year>2017</Year>
<Month>07</Month>
<Day>12</Day>
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<affiliations><list><country><li>Corée du Sud</li>
</country>
<region><li>Région capitale de Séoul</li>
</region>
<settlement><li>Séoul</li>
</settlement>
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<tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Huh, Hee Jae" sort="Huh, Hee Jae" uniqKey="Huh H" first="Hee Jae" last="Huh">Hee Jae Huh</name>
</region>
<name sortKey="Ki, Chang Seok" sort="Ki, Chang Seok" uniqKey="Ki C" first="Chang Seok" last="Ki">Chang Seok Ki</name>
<name sortKey="Kim, Ji Youn" sort="Kim, Ji Youn" uniqKey="Kim J" first="Ji Youn" last="Kim">Ji Youn Kim</name>
<name sortKey="Kim, Jong Won" sort="Kim, Jong Won" uniqKey="Kim J" first="Jong Won" last="Kim">Jong Won Kim</name>
<name sortKey="Kwon, Hyeon Jeong" sort="Kwon, Hyeon Jeong" uniqKey="Kwon H" first="Hyeon Jeong" last="Kwon">Hyeon Jeong Kwon</name>
<name sortKey="Lee, Myoung Keun" sort="Lee, Myoung Keun" uniqKey="Lee M" first="Myoung Keun" last="Lee">Myoung Keun Lee</name>
<name sortKey="Lee, Nam Yong" sort="Lee, Nam Yong" uniqKey="Lee N" first="Nam Yong" last="Lee">Nam Yong Lee</name>
<name sortKey="Yun, Sun Ae" sort="Yun, Sun Ae" uniqKey="Yun S" first="Sun Ae" last="Yun">Sun Ae Yun</name>
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