Performance Evaluation of the PowerChek MERS (upE & ORF1a) Real-Time PCR Kit for the Detection of Middle East Respiratory Syndrome Coronavirus RNA.
Identifieur interne : 000B74 ( PubMed/Checkpoint ); précédent : 000B73; suivant : 000B75Performance Evaluation of the PowerChek MERS (upE & ORF1a) Real-Time PCR Kit for the Detection of Middle East Respiratory Syndrome Coronavirus RNA.
Auteurs : Hee Jae Huh [Corée du Sud] ; Ji Youn Kim [Corée du Sud] ; Hyeon Jeong Kwon [Corée du Sud] ; Sun Ae Yun [Corée du Sud] ; Myoung Keun Lee [Corée du Sud] ; Chang Seok Ki [Corée du Sud] ; Nam Yong Lee [Corée du Sud] ; Jong Won Kim [Corée du Sud]Source :
- Annals of laboratory medicine [ 2234-3814 ] ; 2017.
Descripteurs français
- KwdFr :
- ARN viral (analyse), ARN viral (métabolisme), Analyse de séquence d'ARN, Cadres ouverts de lecture (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Expectoration (virologie), Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Partie nasale du pharynx (virologie), Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Trousses de réactifs pour diagnostic.
- MESH :
- analyse : ARN viral.
- diagnostic : Infections à coronavirus.
- génétique : Cadres ouverts de lecture, Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- métabolisme : ARN viral.
- virologie : Expectoration, Infections à coronavirus, Partie nasale du pharynx.
- Analyse de séquence d'ARN, Humains, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Trousses de réactifs pour diagnostic.
English descriptors
- KwdEn :
- Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Nasopharynx (virology), Open Reading Frames (genetics), RNA, Viral (analysis), RNA, Viral (metabolism), Reagent Kits, Diagnostic, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Analysis, RNA, Sputum (virology).
- MESH :
- chemical , analysis : RNA, Viral.
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus, Open Reading Frames.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- chemical , metabolism : RNA, Viral.
- virology : Coronavirus Infections, Nasopharynx, Sputum.
- Humans, Reagent Kits, Diagnostic, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Analysis, RNA.
Abstract
Molecular detection of Middle East respiratory syndrome coronavirus (MERS-CoV) using real-time reverse transcription (rRT)-PCR assays is the method of choice for diagnosis of MERS. We evaluated the performance of the PowerChek MERS (upE & ORF1a) real-time PCR Kit (PowerChek MERS assay; Kogene Biotech, Korea) a one-step rRT-PCR assay for the qualitative detection of MERS-CoV.
DOI: 10.3343/alm.2017.37.6.494
PubMed: 28840986
Affiliations:
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xml:lang="en"><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (virology)</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Nasopharynx (virology)</term><term>Open Reading Frames (genetics)</term><term>RNA, Viral (analysis)</term><term>RNA, Viral (metabolism)</term><term>Reagent Kits, Diagnostic</term><term>Real-Time Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term><term>Sequence Analysis, RNA</term><term>Sputum (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>ARN viral (métabolisme)</term><term>Analyse de séquence d'ARN</term><term>Cadres ouverts de lecture (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Expectoration (virologie)</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (virologie)</term><term>Partie nasale du pharynx (virologie)</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Trousses de réactifs pour diagnostic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term><term>Open Reading Frames</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cadres ouverts de lecture</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Expectoration</term><term>Infections à coronavirus</term><term>Partie nasale du pharynx</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term><term>Nasopharynx</term><term>Sputum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Reagent Kits, Diagnostic</term><term>Real-Time Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term><term>Sequence Analysis, RNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ARN</term><term>Humains</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Trousses de réactifs pour diagnostic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Molecular detection of Middle East respiratory syndrome coronavirus (MERS-CoV) using real-time reverse transcription (rRT)-PCR assays is the method of choice for diagnosis of MERS. We evaluated the performance of the PowerChek MERS (upE & ORF1a) real-time PCR Kit (PowerChek MERS assay; Kogene Biotech, Korea) a one-step rRT-PCR assay for the qualitative detection of MERS-CoV.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28840986</PMID><DateCompleted><Year>2018</Year><Month>05</Month><Day>11</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">2234-3814</ISSN><JournalIssue CitedMedium="Internet"><Volume>37</Volume><Issue>6</Issue><PubDate><Year>2017</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Annals of laboratory medicine</Title><ISOAbbreviation>Ann Lab Med</ISOAbbreviation></Journal><ArticleTitle>Performance Evaluation of the PowerChek MERS (upE & ORF1a) Real-Time PCR Kit for the Detection of Middle East Respiratory Syndrome Coronavirus RNA.</ArticleTitle><Pagination><MedlinePgn>494-498</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3343/alm.2017.37.6.494</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Molecular detection of Middle East respiratory syndrome coronavirus (MERS-CoV) using real-time reverse transcription (rRT)-PCR assays is the method of choice for diagnosis of MERS. We evaluated the performance of the PowerChek MERS (upE & ORF1a) real-time PCR Kit (PowerChek MERS assay; Kogene Biotech, Korea) a one-step rRT-PCR assay for the qualitative detection of MERS-CoV.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">We evaluated PowerChek MERS assay performance in comparison with nested RT-PCR and sequencing of the RNA-dependent RNA polymerase (RdRp) and N genes. To evaluate diagnostic sensitivity and specificity, 100 clinical specimens (50 positive and 50 negative for MERS-CoV) were simultaneously tested by using the PowerChek MERS and sequencing assays. Assay performance, including limit of detection and precision, was evaluated in vitro by using MERS-CoV RNA transcripts. Analytical specificity was evaluated with a diverse collection of 16 respiratory virus-positive clinical specimens and 14 respiratory bacterial isolates.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The 95% limits of detection of the PowerChek MERS assay for the upE and the open rading frame (ORF)1a were 16.2 copies/μL and 8.2 copies/μL, respectively. No cross-reactivity was observed. The diagnostic sensitivity and specificity of the PowerChek MERS assay were both 100% (95% confidence interval, 91.1-100%).</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The PowerChek MERS assay is a straightforward and accurate assay for detecting MERS-CoV RNA. The assay will be a useful tool for the rapid diagnosis of MERS and could prove especially important for MERS outbreak control.</AbstractText><CopyrightInformation>© The Korean Society for Laboratory Medicine</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Huh</LastName><ForeName>Hee Jae</ForeName><Initials>HJ</Initials><AffiliationInfo><Affiliation>Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Ji Youn</ForeName><Initials>JY</Initials><AffiliationInfo><Affiliation>Center for Clinical Medicine, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kwon</LastName><ForeName>Hyeon Jeong</ForeName><Initials>HJ</Initials><AffiliationInfo><Affiliation>Center for Clinical Medicine, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yun</LastName><ForeName>Sun Ae</ForeName><Initials>SA</Initials><AffiliationInfo><Affiliation>Center for Clinical Medicine, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Myoung Keun</ForeName><Initials>MK</Initials><AffiliationInfo><Affiliation>Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ki</LastName><ForeName>Chang Seok</ForeName><Initials>CS</Initials><AffiliationInfo><Affiliation>Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. changski@skku.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Nam Yong</ForeName><Initials>NY</Initials><AffiliationInfo><Affiliation>Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Jong Won</ForeName><Initials>JW</Initials><AffiliationInfo><Affiliation>Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Korea (South)</Country><MedlineTA>Ann Lab Med</MedlineTA><NlmUniqueID>101571172</NlmUniqueID><ISSNLinking>2234-3806</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011933">Reagent Kits, Diagnostic</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009305" MajorTopicYN="N">Nasopharynx</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016366" MajorTopicYN="N">Open Reading Frames</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011933" MajorTopicYN="N">Reagent Kits, Diagnostic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017423" MajorTopicYN="N">Sequence Analysis, RNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013183" MajorTopicYN="N">Sputum</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">MERS-CoV</Keyword><Keyword MajorTopicYN="N">Performance</Keyword><Keyword MajorTopicYN="N">Real-time reverse-transcription PCR</Keyword></KeywordList><CoiStatement>No potential conflicts of interest relevant to this article were reported.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>02</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>03</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>07</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>8</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>8</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28840986</ArticleId><ArticleId IdType="pii">37.494</ArticleId><ArticleId IdType="doi">10.3343/alm.2017.37.6.494</ArticleId><ArticleId IdType="pmc">PMC5587821</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nat Med. 2016 Jul 7;22(7):701-5</Citation><ArticleIdList><ArticleId IdType="pubmed">27387881</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2012 Sep 27;17(39):null</Citation><ArticleIdList><ArticleId 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Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Huh, Hee Jae" sort="Huh, Hee Jae" uniqKey="Huh H" first="Hee Jae" last="Huh">Hee Jae Huh</name></region><name sortKey="Ki, Chang Seok" sort="Ki, Chang Seok" uniqKey="Ki C" first="Chang Seok" last="Ki">Chang Seok Ki</name><name sortKey="Kim, Ji Youn" sort="Kim, Ji Youn" uniqKey="Kim J" first="Ji Youn" last="Kim">Ji Youn Kim</name><name sortKey="Kim, Jong Won" sort="Kim, Jong Won" uniqKey="Kim J" first="Jong Won" last="Kim">Jong Won Kim</name><name sortKey="Kwon, Hyeon Jeong" sort="Kwon, Hyeon Jeong" uniqKey="Kwon H" first="Hyeon Jeong" last="Kwon">Hyeon Jeong Kwon</name><name sortKey="Lee, Myoung Keun" sort="Lee, Myoung Keun" uniqKey="Lee M" first="Myoung Keun" last="Lee">Myoung Keun Lee</name><name sortKey="Lee, Nam Yong" sort="Lee, Nam Yong" uniqKey="Lee N" first="Nam Yong" last="Lee">Nam Yong Lee</name><name sortKey="Yun, Sun Ae" sort="Yun, Sun Ae" uniqKey="Yun S" first="Sun Ae" last="Yun">Sun Ae Yun</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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