MersV1, PubMed, Checkpoint, bibRecord, 000A47

A Human DPP4-Knockin Mouse's Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV.

Identifieur interne : 000A47 ( PubMed/Checkpoint ); précédent : 000A46; suivant : 000A48

A Human DPP4-Knockin Mouse's Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV.

Source :

Viruses [ 1999-4915 ] ; 2018.

RBID : pubmed:30142928

Descripteurs français

KwdFr :
- Animaux, Anticorps antiviraux (administration et posologie), Anticorps neutralisants (administration et posologie), Clustered regularly interspaced short palindromic repeats, Coronavirus du syndrome respiratoire du Moyen-Orient (croissance et développement), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Dipeptidyl peptidase 4 (génétique), Dipeptidyl peptidase 4 (métabolisme), Endonucleases (métabolisme), Humains, Infections à coronavirus (), Infections à coronavirus (immunologie), Infections à coronavirus (virologie), Modèles animaux de maladie humaine, Protéines bactériennes (métabolisme), Recombinaison génétique, Souris, Susceptibilité à une maladie, Techniques de knock-in de gènes.
MESH :
- administration et posologie : Anticorps antiviraux, Anticorps neutralisants.
- croissance et développement : Coronavirus du syndrome respiratoire du Moyen-Orient.
- génétique : Dipeptidyl peptidase 4.
- immunologie : Coronavirus du syndrome respiratoire du Moyen-Orient, Infections à coronavirus.
- métabolisme : Dipeptidyl peptidase 4, Endonucleases, Protéines bactériennes.
- virologie : Infections à coronavirus.
- Animaux, Clustered regularly interspaced short palindromic repeats, Humains, Infections à coronavirus, Modèles animaux de maladie humaine, Recombinaison génétique, Souris, Susceptibilité à une maladie, Techniques de knock-in de gènes.

English descriptors

KwdEn :
- Animals, Antibodies, Neutralizing (administration & dosage), Antibodies, Viral (administration & dosage), Bacterial Proteins (metabolism), CRISPR-Associated Protein 9, Clustered Regularly Interspaced Short Palindromic Repeats, Coronavirus Infections (immunology), Coronavirus Infections (prevention & control), Coronavirus Infections (virology), Dipeptidyl Peptidase 4 (genetics), Dipeptidyl Peptidase 4 (metabolism), Disease Models, Animal, Disease Susceptibility, Endonucleases (metabolism), Gene Knock-In Techniques, Humans, Mice, Middle East Respiratory Syndrome Coronavirus (growth & development), Middle East Respiratory Syndrome Coronavirus (immunology), Recombination, Genetic.
MESH :
- chemical , administration & dosage : Antibodies, Neutralizing, Antibodies, Viral.
- chemical , genetics : Dipeptidyl Peptidase 4.
- chemical , metabolism : Bacterial Proteins, Dipeptidyl Peptidase 4, Endonucleases.
- growth & development : Middle East Respiratory Syndrome Coronavirus.
- immunology : Coronavirus Infections, Middle East Respiratory Syndrome Coronavirus.
- prevention & control : Coronavirus Infections.
- virology : Coronavirus Infections.
- Animals, CRISPR-Associated Protein 9, Clustered Regularly Interspaced Short Palindromic Repeats, Disease Models, Animal, Disease Susceptibility, Gene Knock-In Techniques, Humans, Mice, Recombination, Genetic.

Abstract

Infection by the Middle East respiratory syndrome coronavirus (MERS-CoV) causes respiratory illness and has a high mortality rate (~35%). The requirement for the virus to be manipulated in a biosafety level three (BSL-3) facility has impeded development of urgently-needed antiviral agents. Here, we established anovel mouse model by inserting human dipeptidyl peptidase 4 (hDPP4) into the Rosa26 locus using CRISPR/Cas9, resulting in global expression of the transgene in a genetically stable mouse line. The mice were highly susceptible to infection by MERS-CoV clinical strain hCoV-EMC, which induced severe diffuse pulmonary disease in the animals, and could also be infected by an optimized pseudotyped MERS-CoV. Administration of the neutralizing monoclonal antibodies, H111-1 and m336, as well as a fusion inhibitor peptide, HR2P-M2, protected mice from challenge with authentic and pseudotyped MERS-CoV. These results confirmed that the hDPP4-knockin mouse is a novel model for studies of MERS-CoV pathogenesis and anti-MERS-CoV antiviral agents in BSL-3 and BSL-2facilities, respectively.

DOI: 10.3390/v10090448
PubMed: 30142928

Affiliations:

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to stream PubMed, to step Corpus: 000801
to stream PubMed, to step Curation: 000801

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Le document en format XML

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<author><name sortKey="Lu, Jianjun" sort="Lu, Jianjun" uniqKey="Lu J" first="Jianjun" last="Lu">Jianjun Lu</name>
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<author><name sortKey="Cao, Yuan" sort="Cao, Yuan" uniqKey="Cao Y" first="Yuan" last="Cao">Yuan Cao</name>
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<author><name sortKey="Huang, Weijin" sort="Huang, Weijin" uniqKey="Huang W" first="Weijin" last="Huang">Weijin Huang</name>
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<author><name sortKey="Ying, Tianlei" sort="Ying, Tianlei" uniqKey="Ying T" first="Tianlei" last="Ying">Tianlei Ying</name>
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<author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name>
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<wicri:regionArea>Key Laboratory of Medical Molecular Virology of the Ministries of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032</wicri:regionArea>
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<author><name sortKey="Wang, Youchun" sort="Wang, Youchun" uniqKey="Wang Y" first="Youchun" last="Wang">Youchun Wang</name>
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<author><name sortKey="Fan, Changfa" sort="Fan, Changfa" uniqKey="Fan C" first="Changfa" last="Fan">Changfa Fan</name>
<affiliation wicri:level="1"><nlm:affiliation>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China. fancf@nifdc.org.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050</wicri:regionArea>
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<author><name sortKey="Wu, Xi" sort="Wu, Xi" uniqKey="Wu X" first="Xi" last="Wu">Xi Wu</name>
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<author><name sortKey="Liu, Qiang" sort="Liu, Qiang" uniqKey="Liu Q" first="Qiang" last="Liu">Qiang Liu</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Division of HIV/AIDS and Sex-Transmitted Virus Vaccines, National Institutes for Food and Drug Control, Beijing 100050</wicri:regionArea>
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<author><name sortKey="Li, Qianqian" sort="Li, Qianqian" uniqKey="Li Q" first="Qianqian" last="Li">Qianqian Li</name>
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<country xml:lang="fr">République populaire de Chine</country>
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<author><name sortKey="Lu, Jianjun" sort="Lu, Jianjun" uniqKey="Lu J" first="Jianjun" last="Lu">Jianjun Lu</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Center for Safety Evaluation of Drugs, Institute for Food and Drug Safety Evaluation, National Institutes for Food and Drug Control, A8 Hongda Middle Street, Beijing Economic-Technological Development Area, Beijing 100176</wicri:regionArea>
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<author><name sortKey="Yang, Yanwei" sort="Yang, Yanwei" uniqKey="Yang Y" first="Yanwei" last="Yang">Yanwei Yang</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Center for Safety Evaluation of Drugs, Institute for Food and Drug Safety Evaluation, National Institutes for Food and Drug Control, A8 Hongda Middle Street, Beijing Economic-Technological Development Area, Beijing 100176</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
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<author><name sortKey="Cao, Yuan" sort="Cao, Yuan" uniqKey="Cao Y" first="Yuan" last="Cao">Yuan Cao</name>
<affiliation wicri:level="1"><nlm:affiliation>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China. caoyuan0512@163.com.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Huang, Weijin" sort="Huang, Weijin" uniqKey="Huang W" first="Weijin" last="Huang">Weijin Huang</name>
<affiliation wicri:level="1"><nlm:affiliation>Division of HIV/AIDS and Sex-Transmitted Virus Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China. huangweijin@nifdc.org.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Division of HIV/AIDS and Sex-Transmitted Virus Vaccines, National Institutes for Food and Drug Control, Beijing 100050</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Liang, Chunnan" sort="Liang, Chunnan" uniqKey="Liang C" first="Chunnan" last="Liang">Chunnan Liang</name>
<affiliation wicri:level="1"><nlm:affiliation>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China. chunnan_liang@nifdc.org.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
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<author><name sortKey="Ying, Tianlei" sort="Ying, Tianlei" uniqKey="Ying T" first="Tianlei" last="Ying">Tianlei Ying</name>
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<country xml:lang="fr">République populaire de Chine</country>
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<wicri:noRegion>Shanghai 200032</wicri:noRegion>
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</author>
<author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name>
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<country xml:lang="fr">République populaire de Chine</country>
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<wicri:noRegion>Shanghai 200032</wicri:noRegion>
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</author>
<author><name sortKey="Wang, Youchun" sort="Wang, Youchun" uniqKey="Wang Y" first="Youchun" last="Wang">Youchun Wang</name>
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<term>Antibodies, Neutralizing (administration & dosage)</term>
<term>Antibodies, Viral (administration & dosage)</term>
<term>Bacterial Proteins (metabolism)</term>
<term>CRISPR-Associated Protein 9</term>
<term>Clustered Regularly Interspaced Short Palindromic Repeats</term>
<term>Coronavirus Infections (immunology)</term>
<term>Coronavirus Infections (prevention & control)</term>
<term>Coronavirus Infections (virology)</term>
<term>Dipeptidyl Peptidase 4 (genetics)</term>
<term>Dipeptidyl Peptidase 4 (metabolism)</term>
<term>Disease Models, Animal</term>
<term>Disease Susceptibility</term>
<term>Endonucleases (metabolism)</term>
<term>Gene Knock-In Techniques</term>
<term>Humans</term>
<term>Mice</term>
<term>Middle East Respiratory Syndrome Coronavirus (growth & development)</term>
<term>Middle East Respiratory Syndrome Coronavirus (immunology)</term>
<term>Recombination, Genetic</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps antiviraux (administration et posologie)</term>
<term>Anticorps neutralisants (administration et posologie)</term>
<term>Clustered regularly interspaced short palindromic repeats</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (croissance et développement)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term>
<term>Dipeptidyl peptidase 4 (génétique)</term>
<term>Dipeptidyl peptidase 4 (métabolisme)</term>
<term>Endonucleases (métabolisme)</term>
<term>Humains</term>
<term>Infections à coronavirus ()</term>
<term>Infections à coronavirus (immunologie)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Modèles animaux de maladie humaine</term>
<term>Protéines bactériennes (métabolisme)</term>
<term>Recombinaison génétique</term>
<term>Souris</term>
<term>Susceptibilité à une maladie</term>
<term>Techniques de knock-in de gènes</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Antibodies, Neutralizing</term>
<term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Dipeptidyl Peptidase 4</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term>
<term>Dipeptidyl Peptidase 4</term>
<term>Endonucleases</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Anticorps neutralisants</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Dipeptidyl peptidase 4</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dipeptidyl peptidase 4</term>
<term>Endonucleases</term>
<term>Protéines bactériennes</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>CRISPR-Associated Protein 9</term>
<term>Clustered Regularly Interspaced Short Palindromic Repeats</term>
<term>Disease Models, Animal</term>
<term>Disease Susceptibility</term>
<term>Gene Knock-In Techniques</term>
<term>Humans</term>
<term>Mice</term>
<term>Recombination, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Clustered regularly interspaced short palindromic repeats</term>
<term>Humains</term>
<term>Infections à coronavirus</term>
<term>Modèles animaux de maladie humaine</term>
<term>Recombinaison génétique</term>
<term>Souris</term>
<term>Susceptibilité à une maladie</term>
<term>Techniques de knock-in de gènes</term>
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<front><div type="abstract" xml:lang="en">Infection by the Middle East respiratory syndrome coronavirus (MERS-CoV) causes respiratory illness and has a high mortality rate (~35%). The requirement for the virus to be manipulated in a biosafety level three (BSL-3) facility has impeded development of urgently-needed antiviral agents. Here, we established anovel mouse model by inserting human dipeptidyl peptidase 4 (hDPP4) into the Rosa26 locus using CRISPR/Cas9, resulting in global expression of the transgene in a genetically stable mouse line. The mice were highly susceptible to infection by MERS-CoV clinical strain hCoV-EMC, which induced severe diffuse pulmonary disease in the animals, and could also be infected by an optimized pseudotyped MERS-CoV. Administration of the neutralizing monoclonal antibodies, H111-1 and m336, as well as a fusion inhibitor peptide, HR2P-M2, protected mice from challenge with authentic and pseudotyped MERS-CoV. These results confirmed that the hDPP4-knockin mouse is a novel model for studies of MERS-CoV pathogenesis and anti-MERS-CoV antiviral agents in BSL-3 and BSL-2facilities, respectively.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30142928</PMID>
<DateCompleted><Year>2018</Year>
<Month>11</Month>
<Day>12</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>12</Month>
<Day>02</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1999-4915</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>10</Volume>
<Issue>9</Issue>
<PubDate><Year>2018</Year>
<Month>08</Month>
<Day>23</Day>
</PubDate>
</JournalIssue>
<Title>Viruses</Title>
<ISOAbbreviation>Viruses</ISOAbbreviation>
</Journal>
<ArticleTitle>A Human DPP4-Knockin Mouse's Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV.</ArticleTitle>
<ELocationID EIdType="pii" ValidYN="Y">E448</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.3390/v10090448</ELocationID>
<Abstract><AbstractText>Infection by the Middle East respiratory syndrome coronavirus (MERS-CoV) causes respiratory illness and has a high mortality rate (~35%). The requirement for the virus to be manipulated in a biosafety level three (BSL-3) facility has impeded development of urgently-needed antiviral agents. Here, we established anovel mouse model by inserting human dipeptidyl peptidase 4 (hDPP4) into the Rosa26 locus using CRISPR/Cas9, resulting in global expression of the transgene in a genetically stable mouse line. The mice were highly susceptible to infection by MERS-CoV clinical strain hCoV-EMC, which induced severe diffuse pulmonary disease in the animals, and could also be infected by an optimized pseudotyped MERS-CoV. Administration of the neutralizing monoclonal antibodies, H111-1 and m336, as well as a fusion inhibitor peptide, HR2P-M2, protected mice from challenge with authentic and pseudotyped MERS-CoV. These results confirmed that the hDPP4-knockin mouse is a novel model for studies of MERS-CoV pathogenesis and anti-MERS-CoV antiviral agents in BSL-3 and BSL-2facilities, respectively.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fan</LastName>
<ForeName>Changfa</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China. fancf@nifdc.org.cn.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Xi</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China. wuxi@nifdc.org.cn.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Qiang</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>Division of HIV/AIDS and Sex-Transmitted Virus Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China. liuqiang@nifdc.org.cn.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Qianqian</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>Division of HIV/AIDS and Sex-Transmitted Virus Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China. liqianqian1199@163.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Susu</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China. liususu@nifdc.org.cn.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lu</LastName>
<ForeName>Jianjun</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>National Center for Safety Evaluation of Drugs, Institute for Food and Drug Safety Evaluation, National Institutes for Food and Drug Control, A8 Hongda Middle Street, Beijing Economic-Technological Development Area, Beijing 100176, China. lujianjun@nifdc.org.cn.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Yanwei</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>National Center for Safety Evaluation of Drugs, Institute for Food and Drug Safety Evaluation, National Institutes for Food and Drug Control, A8 Hongda Middle Street, Beijing Economic-Technological Development Area, Beijing 100176, China. yangyanwei@nifdc.org.cn.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cao</LastName>
<ForeName>Yuan</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China. caoyuan0512@163.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Huang</LastName>
<ForeName>Weijin</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>Division of HIV/AIDS and Sex-Transmitted Virus Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China. huangweijin@nifdc.org.cn.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Liang</LastName>
<ForeName>Chunnan</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing 100050, China. chunnan_liang@nifdc.org.cn.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ying</LastName>
<ForeName>Tianlei</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Key Laboratory of Medical Molecular Virology of the Ministries of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China. tlying@fudan.edu.cn.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jiang</LastName>
<ForeName>Shibo</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Key Laboratory of Medical Molecular Virology of the Ministries of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China. shibojiang@fudan.edu.cn.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Youchun</ForeName>
<Initials>Y</Initials>
<Identifier Source="ORCID">0000-0001-9769-5141</Identifier>
<AffiliationInfo><Affiliation>Division of HIV/AIDS and Sex-Transmitted Virus Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China. wangyc@nifdc.org.cn.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2018</Year>
<Month>08</Month>
<Day>23</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Switzerland</Country>
<MedlineTA>Viruses</MedlineTA>
<NlmUniqueID>101509722</NlmUniqueID>
<ISSNLinking>1999-4915</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D057134">Antibodies, Neutralizing</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.1.-</RegistryNumber>
<NameOfSubstance UI="D000076987">CRISPR-Associated Protein 9</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.1.-</RegistryNumber>
<NameOfSubstance UI="C000606107">Cas9 endonuclease Streptococcus pyogenes</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.1.-</RegistryNumber>
<NameOfSubstance UI="D004720">Endonucleases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.14.5</RegistryNumber>
<NameOfSubstance UI="C042807">DPP4 protein, human</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.14.5</RegistryNumber>
<NameOfSubstance UI="D018819">Dipeptidyl Peptidase 4</NameOfSubstance>
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<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057134" MajorTopicYN="N">Antibodies, Neutralizing</DescriptorName>
<QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName>
<QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName>
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<MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName>
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</MeshHeading>
<MeshHeading><DescriptorName UI="D000076987" MajorTopicYN="N">CRISPR-Associated Protein 9</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D064112" MajorTopicYN="N">Clustered Regularly Interspaced Short Palindromic Repeats</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018819" MajorTopicYN="N">Dipeptidyl Peptidase 4</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004195" MajorTopicYN="Y">Disease Models, Animal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004198" MajorTopicYN="Y">Disease Susceptibility</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004720" MajorTopicYN="N">Endonucleases</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D055879" MajorTopicYN="Y">Gene Knock-In Techniques</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName>
<QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011995" MajorTopicYN="N">Recombination, Genetic</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">MERS-CoV</Keyword>
<Keyword MajorTopicYN="Y">authentic virus</Keyword>
<Keyword MajorTopicYN="Y">hDPP4</Keyword>
<Keyword MajorTopicYN="Y">mouse model</Keyword>
<Keyword MajorTopicYN="Y">pseudotyped virus</Keyword>
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<Month>06</Month>
<Day>24</Day>
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<PubMedPubDate PubStatus="revised"><Year>2018</Year>
<Month>08</Month>
<Day>13</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2018</Year>
<Month>08</Month>
<Day>17</Day>
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<PubMedPubDate PubStatus="entrez"><Year>2018</Year>
<Month>8</Month>
<Day>26</Day>
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<affiliations><list><country><li>République populaire de Chine</li>
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<tree><country name="République populaire de Chine"><noRegion><name sortKey="Fan, Changfa" sort="Fan, Changfa" uniqKey="Fan C" first="Changfa" last="Fan">Changfa Fan</name>
</noRegion>
<name sortKey="Cao, Yuan" sort="Cao, Yuan" uniqKey="Cao Y" first="Yuan" last="Cao">Yuan Cao</name>
<name sortKey="Huang, Weijin" sort="Huang, Weijin" uniqKey="Huang W" first="Weijin" last="Huang">Weijin Huang</name>
<name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name>
<name sortKey="Li, Qianqian" sort="Li, Qianqian" uniqKey="Li Q" first="Qianqian" last="Li">Qianqian Li</name>
<name sortKey="Liang, Chunnan" sort="Liang, Chunnan" uniqKey="Liang C" first="Chunnan" last="Liang">Chunnan Liang</name>
<name sortKey="Liu, Qiang" sort="Liu, Qiang" uniqKey="Liu Q" first="Qiang" last="Liu">Qiang Liu</name>
<name sortKey="Liu, Susu" sort="Liu, Susu" uniqKey="Liu S" first="Susu" last="Liu">Susu Liu</name>
<name sortKey="Lu, Jianjun" sort="Lu, Jianjun" uniqKey="Lu J" first="Jianjun" last="Lu">Jianjun Lu</name>
<name sortKey="Wang, Youchun" sort="Wang, Youchun" uniqKey="Wang Y" first="Youchun" last="Wang">Youchun Wang</name>
<name sortKey="Wu, Xi" sort="Wu, Xi" uniqKey="Wu X" first="Xi" last="Wu">Xi Wu</name>
<name sortKey="Yang, Yanwei" sort="Yang, Yanwei" uniqKey="Yang Y" first="Yanwei" last="Yang">Yanwei Yang</name>
<name sortKey="Ying, Tianlei" sort="Ying, Tianlei" uniqKey="Ying T" first="Tianlei" last="Ying">Tianlei Ying</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A47 | SxmlIndent | more

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Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    PubMed
   |étape=   Checkpoint
   |type=    RBID
   |clé=     pubmed:30142928
   |texte=   A Human DPP4-Knockin Mouse's Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i   -Sk "pubmed:30142928" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

A Human DPP4-Knockin Mouse's Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV.

A Human DPP4-Knockin Mouse's Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV.

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Abstract

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