Accurate differentiation of Escherichia coli and Shigella serogroups: challenges and strategies.
Identifieur interne : 000A26 ( PubMed/Checkpoint ); précédent : 000A25; suivant : 000A27Accurate differentiation of Escherichia coli and Shigella serogroups: challenges and strategies.
Auteurs : N K Devanga Ragupathi [Inde] ; D P Muthuirulandi Sethuvel [Inde] ; F Y Inbanathan [Inde] ; B. Veeraraghavan [Inde]Source :
- New microbes and new infections [ 2052-2975 ] ; 2018.
Abstract
Shigella spp. and Escherichia coli are closely related; both belong to the family Enterobacteriaceae. Phenotypically, Shigella spp. and E. coli share many common characteristics, yet they have separate entities in epidemiology and clinical disease, which poses a diagnostic challenge. We collated information for the best possible approach to differentiate clinically relevant E. coli from Shigella spp. We found that a molecular approach is required for confirmation. High discriminatory potential is seen with whole genome sequencing analysed for k-mers and single nucleotide polymorphism. Among these, identification using single nucleotide polymorphism is easy to perform and analyse, and it thus appears more promising. Among the nonmolecular methods, matrix-assisted desorption ionization-time of flight mass spectrometry may be applicable when data analysis is assisted with advanced analytic tools.
DOI: 10.1016/j.nmni.2017.09.003
PubMed: 29204286
Affiliations:
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<front><div type="abstract" xml:lang="en"><i>Shigella</i>
spp. and <i>Escherichia coli</i>
are closely related; both belong to the family <i>Enterobacteriaceae.</i>
Phenotypically, <i>Shigella</i>
spp. and <i>E. coli</i>
share many common characteristics, yet they have separate entities in epidemiology and clinical disease, which poses a diagnostic challenge. We collated information for the best possible approach to differentiate clinically relevant <i>E. coli</i>
from <i>Shigella</i>
spp. We found that a molecular approach is required for confirmation. High discriminatory potential is seen with whole genome sequencing analysed for k-mers and single nucleotide polymorphism. Among these, identification using single nucleotide polymorphism is easy to perform and analyse, and it thus appears more promising. Among the nonmolecular methods, matrix-assisted desorption ionization-time of flight mass spectrometry may be applicable when data analysis is assisted with advanced analytic tools.</div>
</front>
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<Abstract><AbstractText><i>Shigella</i>
spp. and <i>Escherichia coli</i>
are closely related; both belong to the family <i>Enterobacteriaceae.</i>
Phenotypically, <i>Shigella</i>
spp. and <i>E. coli</i>
share many common characteristics, yet they have separate entities in epidemiology and clinical disease, which poses a diagnostic challenge. We collated information for the best possible approach to differentiate clinically relevant <i>E. coli</i>
from <i>Shigella</i>
spp. We found that a molecular approach is required for confirmation. High discriminatory potential is seen with whole genome sequencing analysed for k-mers and single nucleotide polymorphism. Among these, identification using single nucleotide polymorphism is easy to perform and analyse, and it thus appears more promising. Among the nonmolecular methods, matrix-assisted desorption ionization-time of flight mass spectrometry may be applicable when data analysis is assisted with advanced analytic tools.</AbstractText>
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