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Mining statistically-solid k-mers for accurate NGS error correction.

Identifieur interne : 000832 ( PubMed/Checkpoint ); précédent : 000831; suivant : 000833

Mining statistically-solid k-mers for accurate NGS error correction.

Auteurs : Liang Zhao [République populaire de Chine] ; Jin Xie [République populaire de Chine] ; Lin Bai [République populaire de Chine] ; Wen Chen [République populaire de Chine] ; Mingju Wang [République populaire de Chine] ; Zhonglei Zhang [République populaire de Chine] ; Yiqi Wang [République populaire de Chine] ; Zhe Zhao [République populaire de Chine] ; Jinyan Li [Australie]

Source :

RBID : pubmed:30598110

Descripteurs français

English descriptors

Abstract

NGS data contains many machine-induced errors. The most advanced methods for the error correction heavily depend on the selection of solid k-mers. A solid k-mer is a k-mer frequently occurring in NGS reads. The other k-mers are called weak k-mers. A solid k-mer does not likely contain errors, while a weak k-mer most likely contains errors. An intensively investigated problem is to find a good frequency cutoff f0 to balance the numbers of solid and weak k-mers. Once the cutoff is determined, a more challenging but less-studied problem is to: (i) remove a small subset of solid k-mers that are likely to contain errors, and (ii) add a small subset of weak k-mers, that are likely to contain no errors, into the remaining set of solid k-mers. Identification of these two subsets of k-mers can improve the correction performance.

DOI: 10.1186/s12864-018-5272-y
PubMed: 30598110


Affiliations:


Links toward previous steps (curation, corpus...)


Links to Exploration step

pubmed:30598110

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<div type="abstract" xml:lang="en">NGS data contains many machine-induced errors. The most advanced methods for the error correction heavily depend on the selection of solid k-mers. A solid k-mer is a k-mer frequently occurring in NGS reads. The other k-mers are called weak k-mers. A solid k-mer does not likely contain errors, while a weak k-mer most likely contains errors. An intensively investigated problem is to find a good frequency cutoff f
<sub>0</sub>
to balance the numbers of solid and weak k-mers. Once the cutoff is determined, a more challenging but less-studied problem is to: (i) remove a small subset of solid k-mers that are likely to contain errors, and (ii) add a small subset of weak k-mers, that are likely to contain no errors, into the remaining set of solid k-mers. Identification of these two subsets of k-mers can improve the correction performance.</div>
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<AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">NGS data contains many machine-induced errors. The most advanced methods for the error correction heavily depend on the selection of solid k-mers. A solid k-mer is a k-mer frequently occurring in NGS reads. The other k-mers are called weak k-mers. A solid k-mer does not likely contain errors, while a weak k-mer most likely contains errors. An intensively investigated problem is to find a good frequency cutoff f
<sub>0</sub>
to balance the numbers of solid and weak k-mers. Once the cutoff is determined, a more challenging but less-studied problem is to: (i) remove a small subset of solid k-mers that are likely to contain errors, and (ii) add a small subset of weak k-mers, that are likely to contain no errors, into the remaining set of solid k-mers. Identification of these two subsets of k-mers can improve the correction performance.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">We propose to use a Gamma distribution to model the frequencies of erroneous k-mers and a mixture of Gaussian distributions to model correct k-mers, and combine them to determine f
<sub>0</sub>
. To identify the two special subsets of k-mers, we use the z-score of k-mers which measures the number of standard deviations a k-mer's frequency is from the mean. Then these statistically-solid k-mers are used to construct a Bloom filter for error correction. Our method is markedly superior to the state-of-art methods, tested on both real and synthetic NGS data sets.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The z-score is adequate to distinguish solid k-mers from weak k-mers, particularly useful for pinpointing out solid k-mers having very low frequency. Applying z-score on k-mer can markedly improve the error correction accuracy.</AbstractText>
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