MersV1, PubMed, Checkpoint, bibRecord, 000817

Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10.

Identifieur interne : 000817 ( PubMed/Checkpoint ); précédent : 000816; suivant : 000818

Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10.

Auteurs : James Odame Aboagye [Singapour] ; Chow Wenn Yew [Singapour] ; Oi-Wing Ng [Singapour] ; Vanessa M. Monteil [Suède] ; Ali Mirazimi [Suède] ; Yee-Joo Tan [Singapour]

Source :

Bioscience reports [ 1573-4935 ] ; 2018.

RBID : pubmed:30242057

Descripteurs français

English descriptors

KwdEn :
- A549 Cells, Chemokine CXCL10 (genetics), Coronavirus Infections (genetics), Coronavirus Infections (pathology), Coronavirus Infections (virology), Gene Expression Regulation, Viral (genetics), Host-Pathogen Interactions (genetics), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (pathogenicity), Nucleocapsid Proteins (genetics), Protein Domains (genetics), Transcriptional Activation (genetics).
MESH :
- chemical , genetics : Chemokine CXCL10, Nucleocapsid Proteins.
- genetics : Coronavirus Infections, Gene Expression Regulation, Viral, Host-Pathogen Interactions, Middle East Respiratory Syndrome Coronavirus, Protein Domains, Transcriptional Activation.
- pathogenicity : Middle East Respiratory Syndrome Coronavirus.
- pathology : Coronavirus Infections.
- virology : Coronavirus Infections.
- A549 Cells, Humans.

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) causes respiratory diseases in humans and has a high mortality rate. During infection, MERS-CoV regulates several host cellular processes including antiviral response genes. In order to determine if the nucleocapsid protein of MERS-CoV (MERS-N) plays a role in viral-host interactions, a murine monoclonal antibody was generated so as to allow detection of the protein in infected cells as well as in overexpression system. Then, MERS-N was stably overexpressed in A549 cells, and a PCR array containing 84 genes was used to screen for genes transcriptionally regulated by it. Several up-regulated antiviral genes, namely TNF, IL6, IL8, and CXCL10, were selected for independent validation in transiently transfected 293FT cells. Out of these, the overexpression of MERS-N was found to up-regulate CXCL10 at both transcriptional and translational levels. Interestingly, CXCL10 has been reported to be up-regulated in MERS-CoV infected airway epithelial cells and lung fibroblast cells, as well as monocyte-derived macrophages and dendritic cells. High secretions and persistent increase of CXCL10 in MERS-CoV patients have been also associated with severity of disease. To our knowledge, this is the first report showing that the MERS-N protein is one of the contributing factors for CXCL10 up-regulation during infection. In addition, our results showed that a fragment consisting of residues 196-413 in MERS-N is sufficient to up-regulate CXCL10, while the N-terminal domain and serine-arginine (SR)-rich motif of MERS-N do not play a role in this up-regulation.

DOI: 10.1042/BSR20181059
PubMed: 30242057

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) causes respiratory diseases in humans and has a high mortality rate. During infection, MERS-CoV regulates several host cellular processes including antiviral response genes. In order to determine if the nucleocapsid protein of MERS-CoV (MERS-N) plays a role in viral-host interactions, a murine monoclonal antibody was generated so as to allow detection of the protein in infected cells as well as in overexpression system. Then, MERS-N was stably overexpressed in A549 cells, and a PCR array containing 84 genes was used to screen for genes transcriptionally regulated by it. Several up-regulated antiviral genes, namely <i>TNF, IL6, IL8</i>
, and <i>CXCL10</i>
, were selected for independent validation in transiently transfected 293FT cells. Out of these, the overexpression of MERS-N was found to up-regulate CXCL10 at both transcriptional and translational levels. Interestingly, CXCL10 has been reported to be up-regulated in MERS-CoV infected airway epithelial cells and lung fibroblast cells, as well as monocyte-derived macrophages and dendritic cells. High secretions and persistent increase of CXCL10 in MERS-CoV patients have been also associated with severity of disease. To our knowledge, this is the first report showing that the MERS-N protein is one of the contributing factors for CXCL10 up-regulation during infection. In addition, our results showed that a fragment consisting of residues 196-413 in MERS-N is sufficient to up-regulate CXCL10, while the N-terminal domain and serine-arginine (SR)-rich motif of MERS-N do not play a role in this up-regulation.</div>
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<Abstract><AbstractText>Middle East respiratory syndrome coronavirus (MERS-CoV) causes respiratory diseases in humans and has a high mortality rate. During infection, MERS-CoV regulates several host cellular processes including antiviral response genes. In order to determine if the nucleocapsid protein of MERS-CoV (MERS-N) plays a role in viral-host interactions, a murine monoclonal antibody was generated so as to allow detection of the protein in infected cells as well as in overexpression system. Then, MERS-N was stably overexpressed in A549 cells, and a PCR array containing 84 genes was used to screen for genes transcriptionally regulated by it. Several up-regulated antiviral genes, namely <i>TNF, IL6, IL8</i>
, and <i>CXCL10</i>
, were selected for independent validation in transiently transfected 293FT cells. Out of these, the overexpression of MERS-N was found to up-regulate CXCL10 at both transcriptional and translational levels. Interestingly, CXCL10 has been reported to be up-regulated in MERS-CoV infected airway epithelial cells and lung fibroblast cells, as well as monocyte-derived macrophages and dendritic cells. High secretions and persistent increase of CXCL10 in MERS-CoV patients have been also associated with severity of disease. To our knowledge, this is the first report showing that the MERS-N protein is one of the contributing factors for CXCL10 up-regulation during infection. In addition, our results showed that a fragment consisting of residues 196-413 in MERS-N is sufficient to up-regulate CXCL10, while the N-terminal domain and serine-arginine (SR)-rich motif of MERS-N do not play a role in this up-regulation.</AbstractText>
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<ArticleId IdType="doi">10.1042/BSR20181059</ArticleId>
<ArticleId IdType="pmc">PMC6200698</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Korean Med Sci. 2016 Nov;31(11):1717-1725</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27709848</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Viruses. 2014 Aug 07;6(8):2991-3018</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25105276</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Med Chem. 2014 Mar 27;57(6):2247-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24564608</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Pathol. 2015 Jan;235(2):175-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25294366</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2010 Aug;84(16):8275-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20519402</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2014 May 1;209(9):1331-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24065148</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2013 Jun 1;207(11):1743-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23532101</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virol J. 2015 Dec 22;12:218</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26690369</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Semin Immunopathol. 2017 Jul;39(5):529-539</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28466096</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2014 Apr;454-455:197-205</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24725946</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Wiley Interdiscip Rev RNA. 2016 Mar-Apr;7(2):213-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26749541</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cytokine Growth Factor Rev. 2011 Jun;22(3):121-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21802343</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2013 Dec;94(Pt 12):2679-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24077366</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Struct Biol. 2016 Feb;72(Pt 2):192-202</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26894667</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Microbiol Rev. 2015 Apr;28(2):465-522</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25810418</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2016 Feb;97(2):344-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26602089</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biomed Sci. 2018 May 24;25(1):47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29793506</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2010 Nov;84(21):11575-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20739524</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2014 Feb 14;9(2):e88716</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24551142</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Antimicrob Chemother. 2016 Dec;71(12):3340-3350</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27585965</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2015 Sep;89(17):9029-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26085159</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2018 Apr;517:62-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29199039</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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<name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name>
<name sortKey="Yew, Chow Wenn" sort="Yew, Chow Wenn" uniqKey="Yew C" first="Chow Wenn" last="Yew">Chow Wenn Yew</name>
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<country name="Suède"><noRegion><name sortKey="Monteil, Vanessa M" sort="Monteil, Vanessa M" uniqKey="Monteil V" first="Vanessa M" last="Monteil">Vanessa M. Monteil</name>
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Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10.

Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10.

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