Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10.
Identifieur interne : 000817 ( PubMed/Checkpoint ); précédent : 000816; suivant : 000818Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10.
Auteurs : James Odame Aboagye [Singapour] ; Chow Wenn Yew [Singapour] ; Oi-Wing Ng [Singapour] ; Vanessa M. Monteil [Suède] ; Ali Mirazimi [Suède] ; Yee-Joo Tan [Singapour]Source :
- Bioscience reports [ 1573-4935 ] ; 2018.
Descripteurs français
- KwdFr :
- Activation de la transcription (génétique), Cellules A549, Chimiokine CXCL10 (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité), Domaines protéiques (génétique), Humains, Infections à coronavirus (anatomopathologie), Infections à coronavirus (génétique), Infections à coronavirus (virologie), Interactions hôte-pathogène (génétique), Protéines nucléocapside (génétique), Régulation de l'expression des gènes viraux (génétique).
- MESH :
- anatomopathologie : Infections à coronavirus.
- génétique : Activation de la transcription, Chimiokine CXCL10, Coronavirus du syndrome respiratoire du Moyen-Orient, Domaines protéiques, Infections à coronavirus, Interactions hôte-pathogène, Protéines nucléocapside, Régulation de l'expression des gènes viraux.
- pathogénicité : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Infections à coronavirus.
- Cellules A549, Humains.
English descriptors
- KwdEn :
- A549 Cells, Chemokine CXCL10 (genetics), Coronavirus Infections (genetics), Coronavirus Infections (pathology), Coronavirus Infections (virology), Gene Expression Regulation, Viral (genetics), Host-Pathogen Interactions (genetics), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (pathogenicity), Nucleocapsid Proteins (genetics), Protein Domains (genetics), Transcriptional Activation (genetics).
- MESH :
- chemical , genetics : Chemokine CXCL10, Nucleocapsid Proteins.
- genetics : Coronavirus Infections, Gene Expression Regulation, Viral, Host-Pathogen Interactions, Middle East Respiratory Syndrome Coronavirus, Protein Domains, Transcriptional Activation.
- pathogenicity : Middle East Respiratory Syndrome Coronavirus.
- pathology : Coronavirus Infections.
- virology : Coronavirus Infections.
- A549 Cells, Humans.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) causes respiratory diseases in humans and has a high mortality rate. During infection, MERS-CoV regulates several host cellular processes including antiviral response genes. In order to determine if the nucleocapsid protein of MERS-CoV (MERS-N) plays a role in viral-host interactions, a murine monoclonal antibody was generated so as to allow detection of the protein in infected cells as well as in overexpression system. Then, MERS-N was stably overexpressed in A549 cells, and a PCR array containing 84 genes was used to screen for genes transcriptionally regulated by it. Several up-regulated antiviral genes, namely TNF, IL6, IL8, and CXCL10, were selected for independent validation in transiently transfected 293FT cells. Out of these, the overexpression of MERS-N was found to up-regulate CXCL10 at both transcriptional and translational levels. Interestingly, CXCL10 has been reported to be up-regulated in MERS-CoV infected airway epithelial cells and lung fibroblast cells, as well as monocyte-derived macrophages and dendritic cells. High secretions and persistent increase of CXCL10 in MERS-CoV patients have been also associated with severity of disease. To our knowledge, this is the first report showing that the MERS-N protein is one of the contributing factors for CXCL10 up-regulation during infection. In addition, our results showed that a fragment consisting of residues 196-413 in MERS-N is sufficient to up-regulate CXCL10, while the N-terminal domain and serine-arginine (SR)-rich motif of MERS-N do not play a role in this up-regulation.
DOI: 10.1042/BSR20181059
PubMed: 30242057
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:30242057Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10.</title>
<author><name sortKey="Aboagye, James Odame" sort="Aboagye, James Odame" uniqKey="Aboagye J" first="James Odame" last="Aboagye">James Odame Aboagye</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR)</wicri:regionArea>
<wicri:noRegion>Technology and Research (A*STAR)</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yew, Chow Wenn" sort="Yew, Chow Wenn" uniqKey="Yew C" first="Chow Wenn" last="Yew">Chow Wenn Yew</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR)</wicri:regionArea>
<wicri:noRegion>Technology and Research (A*STAR)</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ng, Oi Wing" sort="Ng, Oi Wing" uniqKey="Ng O" first="Oi-Wing" last="Ng">Oi-Wing Ng</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore</wicri:regionArea>
<orgName type="university">Université nationale de Singapour</orgName>
</affiliation>
</author>
<author><name sortKey="Monteil, Vanessa M" sort="Monteil, Vanessa M" uniqKey="Monteil V" first="Vanessa M" last="Monteil">Vanessa M. Monteil</name>
<affiliation wicri:level="1"><nlm:affiliation>Department for Laboratory Medicine, Karolinska Institute and Karolinska Hospital University, Solna, Sweden.</nlm:affiliation>
<country xml:lang="fr">Suède</country>
<wicri:regionArea>Department for Laboratory Medicine, Karolinska Institute and Karolinska Hospital University, Solna</wicri:regionArea>
<wicri:noRegion>Solna</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mirazimi, Ali" sort="Mirazimi, Ali" uniqKey="Mirazimi A" first="Ali" last="Mirazimi">Ali Mirazimi</name>
<affiliation wicri:level="1"><nlm:affiliation>Department for Laboratory Medicine, Karolinska Institute and Karolinska Hospital University, Solna, Sweden.</nlm:affiliation>
<country xml:lang="fr">Suède</country>
<wicri:regionArea>Department for Laboratory Medicine, Karolinska Institute and Karolinska Hospital University, Solna</wicri:regionArea>
<wicri:noRegion>Solna</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore yee_joo_tan@nuhs.edu.sg.</nlm:affiliation>
<country wicri:rule="url">Singapour</country>
<wicri:regionArea>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR)</wicri:regionArea>
<wicri:noRegion>Technology and Research (A*STAR)</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2018">2018</date>
<idno type="RBID">pubmed:30242057</idno>
<idno type="pmid">30242057</idno>
<idno type="doi">10.1042/BSR20181059</idno>
<idno type="wicri:Area/PubMed/Corpus">000773</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000773</idno>
<idno type="wicri:Area/PubMed/Curation">000773</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000773</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000817</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000817</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10.</title>
<author><name sortKey="Aboagye, James Odame" sort="Aboagye, James Odame" uniqKey="Aboagye J" first="James Odame" last="Aboagye">James Odame Aboagye</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR)</wicri:regionArea>
<wicri:noRegion>Technology and Research (A*STAR)</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yew, Chow Wenn" sort="Yew, Chow Wenn" uniqKey="Yew C" first="Chow Wenn" last="Yew">Chow Wenn Yew</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR)</wicri:regionArea>
<wicri:noRegion>Technology and Research (A*STAR)</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ng, Oi Wing" sort="Ng, Oi Wing" uniqKey="Ng O" first="Oi-Wing" last="Ng">Oi-Wing Ng</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore</wicri:regionArea>
<orgName type="university">Université nationale de Singapour</orgName>
</affiliation>
</author>
<author><name sortKey="Monteil, Vanessa M" sort="Monteil, Vanessa M" uniqKey="Monteil V" first="Vanessa M" last="Monteil">Vanessa M. Monteil</name>
<affiliation wicri:level="1"><nlm:affiliation>Department for Laboratory Medicine, Karolinska Institute and Karolinska Hospital University, Solna, Sweden.</nlm:affiliation>
<country xml:lang="fr">Suède</country>
<wicri:regionArea>Department for Laboratory Medicine, Karolinska Institute and Karolinska Hospital University, Solna</wicri:regionArea>
<wicri:noRegion>Solna</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mirazimi, Ali" sort="Mirazimi, Ali" uniqKey="Mirazimi A" first="Ali" last="Mirazimi">Ali Mirazimi</name>
<affiliation wicri:level="1"><nlm:affiliation>Department for Laboratory Medicine, Karolinska Institute and Karolinska Hospital University, Solna, Sweden.</nlm:affiliation>
<country xml:lang="fr">Suède</country>
<wicri:regionArea>Department for Laboratory Medicine, Karolinska Institute and Karolinska Hospital University, Solna</wicri:regionArea>
<wicri:noRegion>Solna</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore yee_joo_tan@nuhs.edu.sg.</nlm:affiliation>
<country wicri:rule="url">Singapour</country>
<wicri:regionArea>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR)</wicri:regionArea>
<wicri:noRegion>Technology and Research (A*STAR)</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Bioscience reports</title>
<idno type="eISSN">1573-4935</idno>
<imprint><date when="2018" type="published">2018</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>A549 Cells</term>
<term>Chemokine CXCL10 (genetics)</term>
<term>Coronavirus Infections (genetics)</term>
<term>Coronavirus Infections (pathology)</term>
<term>Coronavirus Infections (virology)</term>
<term>Gene Expression Regulation, Viral (genetics)</term>
<term>Host-Pathogen Interactions (genetics)</term>
<term>Humans</term>
<term>Middle East Respiratory Syndrome Coronavirus (genetics)</term>
<term>Middle East Respiratory Syndrome Coronavirus (pathogenicity)</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Protein Domains (genetics)</term>
<term>Transcriptional Activation (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Activation de la transcription (génétique)</term>
<term>Cellules A549</term>
<term>Chimiokine CXCL10 (génétique)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité)</term>
<term>Domaines protéiques (génétique)</term>
<term>Humains</term>
<term>Infections à coronavirus (anatomopathologie)</term>
<term>Infections à coronavirus (génétique)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Interactions hôte-pathogène (génétique)</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Régulation de l'expression des gènes viraux (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chemokine CXCL10</term>
<term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus Infections</term>
<term>Gene Expression Regulation, Viral</term>
<term>Host-Pathogen Interactions</term>
<term>Middle East Respiratory Syndrome Coronavirus</term>
<term>Protein Domains</term>
<term>Transcriptional Activation</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Activation de la transcription</term>
<term>Chimiokine CXCL10</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Domaines protéiques</term>
<term>Infections à coronavirus</term>
<term>Interactions hôte-pathogène</term>
<term>Protéines nucléocapside</term>
<term>Régulation de l'expression des gènes viraux</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>A549 Cells</term>
<term>Humans</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Cellules A549</term>
<term>Humains</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) causes respiratory diseases in humans and has a high mortality rate. During infection, MERS-CoV regulates several host cellular processes including antiviral response genes. In order to determine if the nucleocapsid protein of MERS-CoV (MERS-N) plays a role in viral-host interactions, a murine monoclonal antibody was generated so as to allow detection of the protein in infected cells as well as in overexpression system. Then, MERS-N was stably overexpressed in A549 cells, and a PCR array containing 84 genes was used to screen for genes transcriptionally regulated by it. Several up-regulated antiviral genes, namely <i>TNF, IL6, IL8</i>
, and <i>CXCL10</i>
, were selected for independent validation in transiently transfected 293FT cells. Out of these, the overexpression of MERS-N was found to up-regulate CXCL10 at both transcriptional and translational levels. Interestingly, CXCL10 has been reported to be up-regulated in MERS-CoV infected airway epithelial cells and lung fibroblast cells, as well as monocyte-derived macrophages and dendritic cells. High secretions and persistent increase of CXCL10 in MERS-CoV patients have been also associated with severity of disease. To our knowledge, this is the first report showing that the MERS-N protein is one of the contributing factors for CXCL10 up-regulation during infection. In addition, our results showed that a fragment consisting of residues 196-413 in MERS-N is sufficient to up-regulate CXCL10, while the N-terminal domain and serine-arginine (SR)-rich motif of MERS-N do not play a role in this up-regulation.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30242057</PMID>
<DateCompleted><Year>2019</Year>
<Month>05</Month>
<Day>17</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>05</Month>
<Day>17</Day>
</DateRevised>
<Article PubModel="Electronic-Print"><Journal><ISSN IssnType="Electronic">1573-4935</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>38</Volume>
<Issue>5</Issue>
<PubDate><Year>2018</Year>
<Month>10</Month>
<Day>31</Day>
</PubDate>
</JournalIssue>
<Title>Bioscience reports</Title>
<ISOAbbreviation>Biosci. Rep.</ISOAbbreviation>
</Journal>
<ArticleTitle>Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10.</ArticleTitle>
<ELocationID EIdType="pii" ValidYN="Y">BSR20181059</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.1042/BSR20181059</ELocationID>
<Abstract><AbstractText>Middle East respiratory syndrome coronavirus (MERS-CoV) causes respiratory diseases in humans and has a high mortality rate. During infection, MERS-CoV regulates several host cellular processes including antiviral response genes. In order to determine if the nucleocapsid protein of MERS-CoV (MERS-N) plays a role in viral-host interactions, a murine monoclonal antibody was generated so as to allow detection of the protein in infected cells as well as in overexpression system. Then, MERS-N was stably overexpressed in A549 cells, and a PCR array containing 84 genes was used to screen for genes transcriptionally regulated by it. Several up-regulated antiviral genes, namely <i>TNF, IL6, IL8</i>
, and <i>CXCL10</i>
, were selected for independent validation in transiently transfected 293FT cells. Out of these, the overexpression of MERS-N was found to up-regulate CXCL10 at both transcriptional and translational levels. Interestingly, CXCL10 has been reported to be up-regulated in MERS-CoV infected airway epithelial cells and lung fibroblast cells, as well as monocyte-derived macrophages and dendritic cells. High secretions and persistent increase of CXCL10 in MERS-CoV patients have been also associated with severity of disease. To our knowledge, this is the first report showing that the MERS-N protein is one of the contributing factors for CXCL10 up-regulation during infection. In addition, our results showed that a fragment consisting of residues 196-413 in MERS-N is sufficient to up-regulate CXCL10, while the N-terminal domain and serine-arginine (SR)-rich motif of MERS-N do not play a role in this up-regulation.</AbstractText>
<CopyrightInformation>© 2018 The Author(s).</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Aboagye</LastName>
<ForeName>James Odame</ForeName>
<Initials>JO</Initials>
<AffiliationInfo><Affiliation>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, Singapore.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yew</LastName>
<ForeName>Chow Wenn</ForeName>
<Initials>CW</Initials>
<AffiliationInfo><Affiliation>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ng</LastName>
<ForeName>Oi-Wing</ForeName>
<Initials>OW</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, Singapore.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Monteil</LastName>
<ForeName>Vanessa M</ForeName>
<Initials>VM</Initials>
<AffiliationInfo><Affiliation>Department for Laboratory Medicine, Karolinska Institute and Karolinska Hospital University, Solna, Sweden.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Public Health Agency of Sweden, Stockholm, Sweden.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mirazimi</LastName>
<ForeName>Ali</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Department for Laboratory Medicine, Karolinska Institute and Karolinska Hospital University, Solna, Sweden.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Public Health Agency of Sweden, Stockholm, Sweden.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tan</LastName>
<ForeName>Yee-Joo</ForeName>
<Initials>YJ</Initials>
<Identifier Source="ORCID">0000-0002-2278-4497</Identifier>
<AffiliationInfo><Affiliation>Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore yee_joo_tan@nuhs.edu.sg.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, Singapore.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2018</Year>
<Month>10</Month>
<Day>17</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Biosci Rep</MedlineTA>
<NlmUniqueID>8102797</NlmUniqueID>
<ISSNLinking>0144-8463</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C114753">CXCL10 protein, human</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D054357">Chemokine CXCL10</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000072283" MajorTopicYN="N">A549 Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054357" MajorTopicYN="N">Chemokine CXCL10</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015967" MajorTopicYN="N">Gene Expression Regulation, Viral</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000072417" MajorTopicYN="N">Protein Domains</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015533" MajorTopicYN="N">Transcriptional Activation</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">CXCL10</Keyword>
<Keyword MajorTopicYN="Y">Middle East Respiratory Syndrome coronavirus</Keyword>
<Keyword MajorTopicYN="Y">nucleocapsid</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year>
<Month>07</Month>
<Day>01</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2018</Year>
<Month>09</Month>
<Day>16</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2018</Year>
<Month>09</Month>
<Day>18</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2018</Year>
<Month>9</Month>
<Day>23</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2019</Year>
<Month>5</Month>
<Day>18</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2018</Year>
<Month>9</Month>
<Day>23</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">30242057</ArticleId>
<ArticleId IdType="pii">BSR20181059</ArticleId>
<ArticleId IdType="doi">10.1042/BSR20181059</ArticleId>
<ArticleId IdType="pmc">PMC6200698</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Korean Med Sci. 2016 Nov;31(11):1717-1725</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27709848</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Viruses. 2014 Aug 07;6(8):2991-3018</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25105276</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Med Chem. 2014 Mar 27;57(6):2247-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24564608</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Pathol. 2015 Jan;235(2):175-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25294366</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2010 Aug;84(16):8275-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20519402</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2014 May 1;209(9):1331-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24065148</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2013 Jun 1;207(11):1743-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23532101</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virol J. 2015 Dec 22;12:218</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26690369</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Semin Immunopathol. 2017 Jul;39(5):529-539</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28466096</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2014 Apr;454-455:197-205</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24725946</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Wiley Interdiscip Rev RNA. 2016 Mar-Apr;7(2):213-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26749541</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cytokine Growth Factor Rev. 2011 Jun;22(3):121-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21802343</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2013 Dec;94(Pt 12):2679-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24077366</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Struct Biol. 2016 Feb;72(Pt 2):192-202</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26894667</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Microbiol Rev. 2015 Apr;28(2):465-522</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25810418</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2016 Feb;97(2):344-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26602089</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biomed Sci. 2018 May 24;25(1):47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29793506</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2010 Nov;84(21):11575-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20739524</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2014 Feb 14;9(2):e88716</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24551142</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Antimicrob Chemother. 2016 Dec;71(12):3340-3350</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27585965</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2015 Sep;89(17):9029-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26085159</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2018 Apr;517:62-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29199039</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Singapour</li>
<li>Suède</li>
</country>
<orgName><li>Université nationale de Singapour</li>
</orgName>
</list>
<tree><country name="Singapour"><noRegion><name sortKey="Aboagye, James Odame" sort="Aboagye, James Odame" uniqKey="Aboagye J" first="James Odame" last="Aboagye">James Odame Aboagye</name>
</noRegion>
<name sortKey="Ng, Oi Wing" sort="Ng, Oi Wing" uniqKey="Ng O" first="Oi-Wing" last="Ng">Oi-Wing Ng</name>
<name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name>
<name sortKey="Yew, Chow Wenn" sort="Yew, Chow Wenn" uniqKey="Yew C" first="Chow Wenn" last="Yew">Chow Wenn Yew</name>
</country>
<country name="Suède"><noRegion><name sortKey="Monteil, Vanessa M" sort="Monteil, Vanessa M" uniqKey="Monteil V" first="Vanessa M" last="Monteil">Vanessa M. Monteil</name>
</noRegion>
<name sortKey="Mirazimi, Ali" sort="Mirazimi, Ali" uniqKey="Mirazimi A" first="Ali" last="Mirazimi">Ali Mirazimi</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000817 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000817 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= PubMed |étape= Checkpoint |type= RBID |clé= pubmed:30242057 |texte= Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:30242057" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
This area was generated with Dilib version V0.6.33. |