Pathogenicity and Viral Shedding of MERS-CoV in Immunocompromised Rhesus Macaques.
Identifieur interne : 000814 ( PubMed/Checkpoint ); précédent : 000813; suivant : 000815Pathogenicity and Viral Shedding of MERS-CoV in Immunocompromised Rhesus Macaques.
Auteurs : Joseph Prescott [États-Unis] ; Darryl Falzarano [États-Unis] ; Emmie De Wit [États-Unis] ; Kath Hardcastle [États-Unis] ; Friederike Feldmann [États-Unis] ; Elaine Haddock [États-Unis] ; Dana Scott [États-Unis] ; Heinz Feldmann [États-Unis] ; Vincent Jacobus Munster [États-Unis]Source :
- Frontiers in immunology [ 1664-3224 ] ; 2018.
Descripteurs français
- KwdFr :
- ARN viral (isolement et purification), Animaux, Cellules Vero, Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité), Excrétion virale (immunologie), Femelle, Humains, Infections à coronavirus (immunologie), Infections à coronavirus (virologie), Macaca mulatta, Modèles animaux de maladie humaine, Poumon (immunologie), Poumon (virologie), Réplication virale (immunologie), Sujet immunodéprimé (immunologie).
- MESH :
- immunologie : Coronavirus du syndrome respiratoire du Moyen-Orient, Excrétion virale, Infections à coronavirus, Poumon, Réplication virale, Sujet immunodéprimé.
- isolement et purification : ARN viral, Coronavirus du syndrome respiratoire du Moyen-Orient.
- pathogénicité : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Infections à coronavirus, Poumon.
- Animaux, Cellules Vero, Femelle, Humains, Macaca mulatta, Modèles animaux de maladie humaine.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Coronavirus Infections (immunology), Coronavirus Infections (virology), Disease Models, Animal, Female, Host Microbial Interactions (immunology), Humans, Immunocompromised Host (immunology), Lung (immunology), Lung (virology), Macaca mulatta, Middle East Respiratory Syndrome Coronavirus (immunology), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Middle East Respiratory Syndrome Coronavirus (pathogenicity), RNA, Viral (isolation & purification), Vero Cells, Virus Replication (immunology), Virus Shedding (immunology).
- MESH :
- chemical , isolation & purification : RNA, Viral.
- immunology : Coronavirus Infections, Host Microbial Interactions, Immunocompromised Host, Lung, Middle East Respiratory Syndrome Coronavirus, Virus Replication, Virus Shedding.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- pathogenicity : Middle East Respiratory Syndrome Coronavirus.
- virology : Coronavirus Infections, Lung.
- Animals, Chlorocebus aethiops, Disease Models, Animal, Female, Humans, Macaca mulatta, Vero Cells.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) has recently emerged in the Middle East. Since 2012, there have been approximately 2,100 confirmed cases, with a 35% case fatality rate. Disease severity has been linked to patient health status, as people with chronic diseases or an immunocompromised status fare worse, although the mechanisms of disease have yet to be elucidated. We used the rhesus macaque model of mild MERS to investigate whether the immune response plays a role in the pathogenicity in relation to MERS-CoV shedding. Immunosuppressed macaques were inoculated with MERS-CoV and sampled daily for 6 days to assess their immune statues and to measure viral shedding and replication. Immunosuppressed macaques supported significantly higher levels of MERS-CoV replication in respiratory tissues and shed more virus, and virus disseminated to tissues outside of the respiratory tract, whereas viral RNA was confined to respiratory tissues in non-immunosuppressed animals. Despite increased viral replication, pathology in the lungs was significantly lower in immunosuppressed animals. The observation that the virus was less pathogenic in these animals suggests that disease has an immunopathogenic component and shows that inflammatory responses elicited by the virus contribute to disease.
DOI: 10.3389/fimmu.2018.00205
PubMed: 29483914
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:29483914Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Pathogenicity and Viral Shedding of MERS-CoV in Immunocompromised Rhesus Macaques.</title>
<author><name sortKey="Prescott, Joseph" sort="Prescott, Joseph" uniqKey="Prescott J" first="Joseph" last="Prescott">Joseph Prescott</name>
<affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Falzarano, Darryl" sort="Falzarano, Darryl" uniqKey="Falzarano D" first="Darryl" last="Falzarano">Darryl Falzarano</name>
<affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="De Wit, Emmie" sort="De Wit, Emmie" uniqKey="De Wit E" first="Emmie" last="De Wit">Emmie De Wit</name>
<affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hardcastle, Kath" sort="Hardcastle, Kath" uniqKey="Hardcastle K" first="Kath" last="Hardcastle">Kath Hardcastle</name>
<affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Feldmann, Friederike" sort="Feldmann, Friederike" uniqKey="Feldmann F" first="Friederike" last="Feldmann">Friederike Feldmann</name>
<affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Haddock, Elaine" sort="Haddock, Elaine" uniqKey="Haddock E" first="Elaine" last="Haddock">Elaine Haddock</name>
<affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Scott, Dana" sort="Scott, Dana" uniqKey="Scott D" first="Dana" last="Scott">Dana Scott</name>
<affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name>
<affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Munster, Vincent Jacobus" sort="Munster, Vincent Jacobus" uniqKey="Munster V" first="Vincent Jacobus" last="Munster">Vincent Jacobus Munster</name>
<affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2018">2018</date>
<idno type="RBID">pubmed:29483914</idno>
<idno type="pmid">29483914</idno>
<idno type="doi">10.3389/fimmu.2018.00205</idno>
<idno type="wicri:Area/PubMed/Corpus">000991</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000991</idno>
<idno type="wicri:Area/PubMed/Curation">000991</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000991</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000814</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000814</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Pathogenicity and Viral Shedding of MERS-CoV in Immunocompromised Rhesus Macaques.</title>
<author><name sortKey="Prescott, Joseph" sort="Prescott, Joseph" uniqKey="Prescott J" first="Joseph" last="Prescott">Joseph Prescott</name>
<affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Falzarano, Darryl" sort="Falzarano, Darryl" uniqKey="Falzarano D" first="Darryl" last="Falzarano">Darryl Falzarano</name>
<affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="De Wit, Emmie" sort="De Wit, Emmie" uniqKey="De Wit E" first="Emmie" last="De Wit">Emmie De Wit</name>
<affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hardcastle, Kath" sort="Hardcastle, Kath" uniqKey="Hardcastle K" first="Kath" last="Hardcastle">Kath Hardcastle</name>
<affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Feldmann, Friederike" sort="Feldmann, Friederike" uniqKey="Feldmann F" first="Friederike" last="Feldmann">Friederike Feldmann</name>
<affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Haddock, Elaine" sort="Haddock, Elaine" uniqKey="Haddock E" first="Elaine" last="Haddock">Elaine Haddock</name>
<affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Scott, Dana" sort="Scott, Dana" uniqKey="Scott D" first="Dana" last="Scott">Dana Scott</name>
<affiliation wicri:level="2"><nlm:affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name>
<affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Munster, Vincent Jacobus" sort="Munster, Vincent Jacobus" uniqKey="Munster V" first="Vincent Jacobus" last="Munster">Vincent Jacobus Munster</name>
<affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea>
<placeName><region type="state">Montana</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Frontiers in immunology</title>
<idno type="ISSN">1664-3224</idno>
<imprint><date when="2018" type="published">2018</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Coronavirus Infections (immunology)</term>
<term>Coronavirus Infections (virology)</term>
<term>Disease Models, Animal</term>
<term>Female</term>
<term>Host Microbial Interactions (immunology)</term>
<term>Humans</term>
<term>Immunocompromised Host (immunology)</term>
<term>Lung (immunology)</term>
<term>Lung (virology)</term>
<term>Macaca mulatta</term>
<term>Middle East Respiratory Syndrome Coronavirus (immunology)</term>
<term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term>
<term>Middle East Respiratory Syndrome Coronavirus (pathogenicity)</term>
<term>RNA, Viral (isolation & purification)</term>
<term>Vero Cells</term>
<term>Virus Replication (immunology)</term>
<term>Virus Shedding (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (isolement et purification)</term>
<term>Animaux</term>
<term>Cellules Vero</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité)</term>
<term>Excrétion virale (immunologie)</term>
<term>Femelle</term>
<term>Humains</term>
<term>Infections à coronavirus (immunologie)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Macaca mulatta</term>
<term>Modèles animaux de maladie humaine</term>
<term>Poumon (immunologie)</term>
<term>Poumon (virologie)</term>
<term>Réplication virale (immunologie)</term>
<term>Sujet immunodéprimé (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Excrétion virale</term>
<term>Infections à coronavirus</term>
<term>Poumon</term>
<term>Réplication virale</term>
<term>Sujet immunodéprimé</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Host Microbial Interactions</term>
<term>Immunocompromised Host</term>
<term>Lung</term>
<term>Middle East Respiratory Syndrome Coronavirus</term>
<term>Virus Replication</term>
<term>Virus Shedding</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Poumon</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Lung</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Disease Models, Animal</term>
<term>Female</term>
<term>Humans</term>
<term>Macaca mulatta</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Femelle</term>
<term>Humains</term>
<term>Macaca mulatta</term>
<term>Modèles animaux de maladie humaine</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) has recently emerged in the Middle East. Since 2012, there have been approximately 2,100 confirmed cases, with a 35% case fatality rate. Disease severity has been linked to patient health status, as people with chronic diseases or an immunocompromised status fare worse, although the mechanisms of disease have yet to be elucidated. We used the rhesus macaque model of mild MERS to investigate whether the immune response plays a role in the pathogenicity in relation to MERS-CoV shedding. Immunosuppressed macaques were inoculated with MERS-CoV and sampled daily for 6 days to assess their immune statues and to measure viral shedding and replication. Immunosuppressed macaques supported significantly higher levels of MERS-CoV replication in respiratory tissues and shed more virus, and virus disseminated to tissues outside of the respiratory tract, whereas viral RNA was confined to respiratory tissues in non-immunosuppressed animals. Despite increased viral replication, pathology in the lungs was significantly lower in immunosuppressed animals. The observation that the virus was less pathogenic in these animals suggests that disease has an immunopathogenic component and shows that inflammatory responses elicited by the virus contribute to disease.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29483914</PMID>
<DateCompleted><Year>2019</Year>
<Month>04</Month>
<Day>03</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-3224</ISSN>
<JournalIssue CitedMedium="Print"><Volume>9</Volume>
<PubDate><Year>2018</Year>
</PubDate>
</JournalIssue>
<Title>Frontiers in immunology</Title>
<ISOAbbreviation>Front Immunol</ISOAbbreviation>
</Journal>
<ArticleTitle>Pathogenicity and Viral Shedding of MERS-CoV in Immunocompromised Rhesus Macaques.</ArticleTitle>
<Pagination><MedlinePgn>205</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.3389/fimmu.2018.00205</ELocationID>
<Abstract><AbstractText>Middle East respiratory syndrome coronavirus (MERS-CoV) has recently emerged in the Middle East. Since 2012, there have been approximately 2,100 confirmed cases, with a 35% case fatality rate. Disease severity has been linked to patient health status, as people with chronic diseases or an immunocompromised status fare worse, although the mechanisms of disease have yet to be elucidated. We used the rhesus macaque model of mild MERS to investigate whether the immune response plays a role in the pathogenicity in relation to MERS-CoV shedding. Immunosuppressed macaques were inoculated with MERS-CoV and sampled daily for 6 days to assess their immune statues and to measure viral shedding and replication. Immunosuppressed macaques supported significantly higher levels of MERS-CoV replication in respiratory tissues and shed more virus, and virus disseminated to tissues outside of the respiratory tract, whereas viral RNA was confined to respiratory tissues in non-immunosuppressed animals. Despite increased viral replication, pathology in the lungs was significantly lower in immunosuppressed animals. The observation that the virus was less pathogenic in these animals suggests that disease has an immunopathogenic component and shows that inflammatory responses elicited by the virus contribute to disease.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Prescott</LastName>
<ForeName>Joseph</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Falzarano</LastName>
<ForeName>Darryl</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>de Wit</LastName>
<ForeName>Emmie</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hardcastle</LastName>
<ForeName>Kath</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Feldmann</LastName>
<ForeName>Friederike</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Haddock</LastName>
<ForeName>Elaine</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Scott</LastName>
<ForeName>Dana</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Rocky Mountain Veterinary Branch, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Feldmann</LastName>
<ForeName>Heinz</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Munster</LastName>
<ForeName>Vincent Jacobus</ForeName>
<Initials>VJ</Initials>
<AffiliationInfo><Affiliation>Laboratory of Virology, Division of Intramural Research, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D052060">Research Support, N.I.H., Intramural</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2018</Year>
<Month>02</Month>
<Day>12</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Switzerland</Country>
<MedlineTA>Front Immunol</MedlineTA>
<NlmUniqueID>101560960</NlmUniqueID>
<ISSNLinking>1664-3224</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004195" MajorTopicYN="N">Disease Models, Animal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000076662" MajorTopicYN="N">Host Microbial Interactions</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016867" MajorTopicYN="N">Immunocompromised Host</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008168" MajorTopicYN="N">Lung</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008253" MajorTopicYN="N">Macaca mulatta</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017201" MajorTopicYN="N">Virus Shedding</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Middle East respiratory syndrome coronavirus</Keyword>
<Keyword MajorTopicYN="Y">immunosuppression</Keyword>
<Keyword MajorTopicYN="Y">macaque monkey</Keyword>
<Keyword MajorTopicYN="Y">pathology</Keyword>
<Keyword MajorTopicYN="Y">shedding</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year>
<Month>11</Month>
<Day>13</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2018</Year>
<Month>01</Month>
<Day>24</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2018</Year>
<Month>2</Month>
<Day>28</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2018</Year>
<Month>2</Month>
<Day>28</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2018</Year>
<Month>2</Month>
<Day>28</Day>
<Hour>6</Hour>
<Minute>1</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">29483914</ArticleId>
<ArticleId IdType="doi">10.3389/fimmu.2018.00205</ArticleId>
<ArticleId IdType="pmc">PMC5816332</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Int J Infect Dis. 2014 Dec;29:301-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25303830</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Pulm Med. 2014 May;20(3):233-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24626235</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Infect Drug Resist. 2014 Nov 03;7:281-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25395865</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin HIV AIDS. 2010 Mar;5(2):189-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20543599</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2012 Nov 8;367 (19):1814-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Chemother. 2017 Nov;23 (11):769-773</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28545936</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Viruses. 2014 May 26;6(5):2214-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24859344</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2014 May 1;209(9):1331-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24065148</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2013 Jun 29;381(9885):2265-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23727167</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Semin Respir Crit Care Med. 2010 Oct;31(5):561-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20941657</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2016 Sep 29;375(13):1303-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27682053</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Pediatr Infect Dis J. 2014 Sep;33(9):904-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24763193</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2012 Dec 06;17(49):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23231891</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Infect Dis. 2016 Dec 10;64(5):551-557</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27940937</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Microbiol. 2016 Aug;14 (8):523-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27344959</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2015 Apr;89(7):3859-69</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25609809</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet Infect Dis. 2013 Sep;13(9):745-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23782859</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16598-603</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24062443</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013 Jul 02;8(7):e69127</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23844250</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2013 Apr 18;368(16):1560-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23550601</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2013 Mar 14;495(7440):251-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23486063</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur Cytokine Netw. 2006 Mar;17(1):4-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16613757</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2013 Aug 1;369(5):407-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23782161</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Am J Pathol. 2016 Mar;186(3):630-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26724387</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunity. 2013 Oct 17;39(4):633-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24138880</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet Respir Med. 2014 Oct;2(10 ):813-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25174549</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Immunol. 2004 Jan;25(1):4-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14698276</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2014 Aug 21;10(8):e1004250</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25144235</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Jun;87(12):6604-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23552422</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Ann Intern Med. 2014 Mar 18;160(6):389-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24474051</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet Infect Dis. 2013 Sep;13(9):752-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23891402</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2013 Feb 19;4(1):e00611-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23422412</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5133-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23479618</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Montana</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Montana"><name sortKey="Prescott, Joseph" sort="Prescott, Joseph" uniqKey="Prescott J" first="Joseph" last="Prescott">Joseph Prescott</name>
</region>
<name sortKey="De Wit, Emmie" sort="De Wit, Emmie" uniqKey="De Wit E" first="Emmie" last="De Wit">Emmie De Wit</name>
<name sortKey="Falzarano, Darryl" sort="Falzarano, Darryl" uniqKey="Falzarano D" first="Darryl" last="Falzarano">Darryl Falzarano</name>
<name sortKey="Feldmann, Friederike" sort="Feldmann, Friederike" uniqKey="Feldmann F" first="Friederike" last="Feldmann">Friederike Feldmann</name>
<name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name>
<name sortKey="Haddock, Elaine" sort="Haddock, Elaine" uniqKey="Haddock E" first="Elaine" last="Haddock">Elaine Haddock</name>
<name sortKey="Hardcastle, Kath" sort="Hardcastle, Kath" uniqKey="Hardcastle K" first="Kath" last="Hardcastle">Kath Hardcastle</name>
<name sortKey="Munster, Vincent Jacobus" sort="Munster, Vincent Jacobus" uniqKey="Munster V" first="Vincent Jacobus" last="Munster">Vincent Jacobus Munster</name>
<name sortKey="Scott, Dana" sort="Scott, Dana" uniqKey="Scott D" first="Dana" last="Scott">Dana Scott</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000814 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000814 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= PubMed |étape= Checkpoint |type= RBID |clé= pubmed:29483914 |texte= Pathogenicity and Viral Shedding of MERS-CoV in Immunocompromised Rhesus Macaques. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:29483914" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |