Development and Evaluation of a Multiplexed Immunoassay for Simultaneous Detection of Serum IgG Antibodies to Six Human Coronaviruses.
Identifieur interne : 000607 ( PubMed/Checkpoint ); précédent : 000606; suivant : 000608Development and Evaluation of a Multiplexed Immunoassay for Simultaneous Detection of Serum IgG Antibodies to Six Human Coronaviruses.
Auteurs : Suvang U. Trivedi [États-Unis] ; Congrong Miao [États-Unis] ; Joseph E. Sanchez [États-Unis] ; Hayat Caidi [États-Unis] ; Azaibi Tamin [États-Unis] ; Lia Haynes [États-Unis] ; Natalie J. Thornburg [États-Unis]Source :
- Scientific reports [ 2045-2322 ] ; 2019.
Abstract
Known human coronaviruses (hCoV) usually cause mild to moderate upper-respiratory tract illnesses, except SARS-CoV and MERS-CoV, which, in addition to mild illness can also be associated with severe respiratory diseases and high mortality rates. Well-characterized multiplexed serologic assays are needed to aid in rapid detection and surveillance of hCoVs. The present study describes development and evaluation of a multiplexed magnetic microsphere immunoassay (MMIA) to simultaneously detect immunoglobulin G (IgG) antibodies specific for recombinant nucleocapsid proteins (recN) from hCoVs 229E, NL63, OC43, HKU1, SARS-CoV, and MERS-CoV. We used paired human sera to screen for IgG with reactivity against six hCoVs to determine assay sensitivity, specificity and reproducibility. We found no signal interference between monoplex and multiplex assay formats (R2 range = 0.87-0.97). Screening of paired human sera using MMIA, resulted in 92 of 106 (sensitivity: 86%) as positive and 68 of 80 (specificity: 84%) as negative. This study serves as a proof of concept that it is feasible to develop and use a multiplexed microsphere immunoassay as a next generation screening tool for use in large scale seroprevalence studies of hCoVs.
DOI: 10.1038/s41598-018-37747-5
PubMed: 30718599
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Trivedi</name><affiliation wicri:level="1"><nlm:affiliation>Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities, 100 ORAU way, Oak Ridge, TN, 37830, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities, 100 ORAU way, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation></author><author><name sortKey="Miao, Congrong" sort="Miao, Congrong" uniqKey="Miao C" first="Congrong" last="Miao">Congrong Miao</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention 1600 Clifton Rd NE, Atlanta, GA, 30329, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention 1600 Clifton Rd NE, Atlanta, GA, 30329</wicri:regionArea><wicri:noRegion>30329</wicri:noRegion></affiliation></author><author><name sortKey="Sanchez, Joseph E" sort="Sanchez, Joseph E" uniqKey="Sanchez J" first="Joseph E" last="Sanchez">Joseph E. 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Thornburg</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention 1600 Clifton Rd NE, Atlanta, GA, 30329, USA. nax3@cdc.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention 1600 Clifton Rd NE, Atlanta, GA, 30329</wicri:regionArea><wicri:noRegion>30329</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Known human coronaviruses (hCoV) usually cause mild to moderate upper-respiratory tract illnesses, except SARS-CoV and MERS-CoV, which, in addition to mild illness can also be associated with severe respiratory diseases and high mortality rates. Well-characterized multiplexed serologic assays are needed to aid in rapid detection and surveillance of hCoVs. The present study describes development and evaluation of a multiplexed magnetic microsphere immunoassay (MMIA) to simultaneously detect immunoglobulin G (IgG) antibodies specific for recombinant nucleocapsid proteins (recN) from hCoVs 229E, NL63, OC43, HKU1, SARS-CoV, and MERS-CoV. We used paired human sera to screen for IgG with reactivity against six hCoVs to determine assay sensitivity, specificity and reproducibility. We found no signal interference between monoplex and multiplex assay formats (R<sup>2</sup> range = 0.87-0.97). Screening of paired human sera using MMIA, resulted in 92 of 106 (sensitivity: 86%) as positive and 68 of 80 (specificity: 84%) as negative. This study serves as a proof of concept that it is feasible to develop and use a multiplexed microsphere immunoassay as a next generation screening tool for use in large scale seroprevalence studies of hCoVs.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">30718599</PMID><DateRevised><Year>2020</Year><Month>03</Month><Day>12</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>02</Month><Day>04</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Development and Evaluation of a Multiplexed Immunoassay for Simultaneous Detection of Serum IgG Antibodies to Six Human Coronaviruses.</ArticleTitle><Pagination><MedlinePgn>1390</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-018-37747-5</ELocationID><Abstract><AbstractText>Known human coronaviruses (hCoV) usually cause mild to moderate upper-respiratory tract illnesses, except SARS-CoV and MERS-CoV, which, in addition to mild illness can also be associated with severe respiratory diseases and high mortality rates. Well-characterized multiplexed serologic assays are needed to aid in rapid detection and surveillance of hCoVs. The present study describes development and evaluation of a multiplexed magnetic microsphere immunoassay (MMIA) to simultaneously detect immunoglobulin G (IgG) antibodies specific for recombinant nucleocapsid proteins (recN) from hCoVs 229E, NL63, OC43, HKU1, SARS-CoV, and MERS-CoV. We used paired human sera to screen for IgG with reactivity against six hCoVs to determine assay sensitivity, specificity and reproducibility. We found no signal interference between monoplex and multiplex assay formats (R<sup>2</sup> range = 0.87-0.97). Screening of paired human sera using MMIA, resulted in 92 of 106 (sensitivity: 86%) as positive and 68 of 80 (specificity: 84%) as negative. This study serves as a proof of concept that it is feasible to develop and use a multiplexed microsphere immunoassay as a next generation screening tool for use in large scale seroprevalence studies of hCoVs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Trivedi</LastName><ForeName>Suvang U</ForeName><Initials>SU</Initials><AffiliationInfo><Affiliation>Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities, 100 ORAU way, Oak Ridge, TN, 37830, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miao</LastName><ForeName>Congrong</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention 1600 Clifton Rd NE, Atlanta, GA, 30329, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sanchez</LastName><ForeName>Joseph E</ForeName><Initials>JE</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention 1600 Clifton Rd NE, Atlanta, GA, 30329, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Caidi</LastName><ForeName>Hayat</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention 1600 Clifton Rd NE, Atlanta, GA, 30329, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tamin</LastName><ForeName>Azaibi</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention 1600 Clifton Rd NE, Atlanta, GA, 30329, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Haynes</LastName><ForeName>Lia</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention 1600 Clifton Rd NE, Atlanta, GA, 30329, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thornburg</LastName><ForeName>Natalie J</ForeName><Initials>NJ</Initials><Identifier Source="ORCID">0000-0003-3523-3616</Identifier><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention 1600 Clifton Rd NE, Atlanta, GA, 30329, USA. nax3@cdc.gov.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>02</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>09</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>12</Month><Day>03</Day></PubMedPubDate><PubMedPubDate 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name="États-Unis"><noRegion><name sortKey="Trivedi, Suvang U" sort="Trivedi, Suvang U" uniqKey="Trivedi S" first="Suvang U" last="Trivedi">Suvang U. Trivedi</name></noRegion><name sortKey="Caidi, Hayat" sort="Caidi, Hayat" uniqKey="Caidi H" first="Hayat" last="Caidi">Hayat Caidi</name><name sortKey="Haynes, Lia" sort="Haynes, Lia" uniqKey="Haynes L" first="Lia" last="Haynes">Lia Haynes</name><name sortKey="Miao, Congrong" sort="Miao, Congrong" uniqKey="Miao C" first="Congrong" last="Miao">Congrong Miao</name><name sortKey="Sanchez, Joseph E" sort="Sanchez, Joseph E" uniqKey="Sanchez J" first="Joseph E" last="Sanchez">Joseph E. Sanchez</name><name sortKey="Tamin, Azaibi" sort="Tamin, Azaibi" uniqKey="Tamin A" first="Azaibi" last="Tamin">Azaibi Tamin</name><name sortKey="Thornburg, Natalie J" sort="Thornburg, Natalie J" uniqKey="Thornburg N" first="Natalie J" last="Thornburg">Natalie J. Thornburg</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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