Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles.
Identifieur interne : 000605 ( PubMed/Checkpoint ); précédent : 000604; suivant : 000606Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles.
Auteurs : Hanbi Kim ; Minseon Park [Corée du Sud] ; Joonki Hwang ; Jin Hwa Kim ; Doo-Ryeon Chung [Corée du Sud] ; Kyu-Sung Lee [Corée du Sud] ; Minhee Kang [Corée du Sud]Source :
- ACS sensors [ 2379-3694 ] ; 2019.
Descripteurs français
- KwdFr :
- Cadres ouverts de lecture (génétique), Colorimétrie (), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Disulfures (), Limite de détection, Nanoparticules métalliques (), Or (), Protéines de l'enveloppe virale (génétique), Techniques de biocapteur ().
- MESH :
- génétique : Cadres ouverts de lecture, Coronavirus du syndrome respiratoire du Moyen-Orient, Protéines de l'enveloppe virale.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- Colorimétrie, Disulfures, Limite de détection, Nanoparticules métalliques, Or, Techniques de biocapteur.
English descriptors
- KwdEn :
- Biosensing Techniques (methods), Colorimetry (methods), Disulfides (chemistry), Gold (chemistry), Limit of Detection, Metal Nanoparticles (chemistry), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Open Reading Frames (genetics), Viral Envelope Proteins (genetics).
- MESH :
- chemical , chemistry : Disulfides, Gold.
- chemistry : Metal Nanoparticles.
- genetics : Middle East Respiratory Syndrome Coronavirus, Open Reading Frames, Viral Envelope Proteins.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Biosensing Techniques, Colorimetry.
- Limit of Detection.
Abstract
Worldwide outbreaks of infectious diseases necessitate the development of rapid and accurate diagnostic methods. Colorimetric assays are a representative tool to simply identify the target molecules in specimens through color changes of an indicator (e.g., nanosized metallic particle, and dye molecules). The detection method is used to confirm the presence of biomarkers visually and measure absorbance of the colored compounds at a specific wavelength. In this study, we propose a colorimetric assay based on an extended form of double-stranded DNA (dsDNA) self-assembly shielded gold nanoparticles (AuNPs) under positive electrolyte (e.g., 0.1 M MgCl2) for detection of Middle East respiratory syndrome coronavirus (MERS-CoV). This platform is able to verify the existence of viral molecules through a localized surface plasmon resonance (LSPR) shift and color changes of AuNPs in the UV-vis wavelength range. We designed a pair of thiol-modified probes at either the 5' end or 3' end to organize complementary base pairs with upstream of the E protein gene (upE) and open reading frames (ORF) 1a on MERS-CoV. The dsDNA of the target and probes forms a disulfide-induced long self-assembled complex, which protects AuNPs from salt-induced aggregation and transition of optical properties. This colorimetric assay could discriminate down to 1 pmol/μL of 30 bp MERS-CoV and further be adapted for convenient on-site detection of other infectious diseases, especially in resource-limited settings.
DOI: 10.1021/acssensors.9b00175
PubMed: 31062580
Affiliations:
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last="Chung">Doo-Ryeon Chung</name><affiliation wicri:level="1"><nlm:affiliation>Asia Pacific Foundation for Infectious Diseases (APFID) , Seoul , Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Asia Pacific Foundation for Infectious Diseases (APFID) , Seoul </wicri:regionArea><wicri:noRegion>Seoul </wicri:noRegion></affiliation></author><author><name sortKey="Lee, Kyu Sung" sort="Lee, Kyu Sung" uniqKey="Lee K" first="Kyu-Sung" last="Lee">Kyu-Sung Lee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Device Management and Research, SAIHST (Samsung Advanced Institute for Health Sciences & Technology) , Sungkyunkwan University , Seoul , Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Medical Device Management and Research, SAIHST (Samsung Advanced Institute for Health Sciences & Technology) , Sungkyunkwan University , Seoul </wicri:regionArea><wicri:noRegion>Seoul </wicri:noRegion></affiliation></author><author><name sortKey="Kang, Minhee" sort="Kang, Minhee" uniqKey="Kang M" first="Minhee" last="Kang">Minhee Kang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Device Management and Research, SAIHST (Samsung Advanced Institute for Health Sciences & Technology) , Sungkyunkwan University , Seoul , Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Medical Device Management and Research, SAIHST (Samsung Advanced Institute for Health Sciences & Technology) , Sungkyunkwan University , Seoul </wicri:regionArea><wicri:noRegion>Seoul </wicri:noRegion></affiliation></author></analytic><series><title level="j">ACS sensors</title><idno type="eISSN">2379-3694</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (methods)</term><term>Colorimetry (methods)</term><term>Disulfides (chemistry)</term><term>Gold (chemistry)</term><term>Limit of Detection</term><term>Metal Nanoparticles (chemistry)</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Open Reading Frames (genetics)</term><term>Viral Envelope Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cadres ouverts de lecture (génétique)</term><term>Colorimétrie ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Disulfures ()</term><term>Limite de détection</term><term>Nanoparticules métalliques ()</term><term>Or ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Techniques de biocapteur ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Disulfides</term><term>Gold</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Metal Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term><term>Open Reading Frames</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cadres ouverts de lecture</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term><term>Colorimetry</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Limit of Detection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Colorimétrie</term><term>Disulfures</term><term>Limite de détection</term><term>Nanoparticules métalliques</term><term>Or</term><term>Techniques de biocapteur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Worldwide outbreaks of infectious diseases necessitate the development of rapid and accurate diagnostic methods. Colorimetric assays are a representative tool to simply identify the target molecules in specimens through color changes of an indicator (e.g., nanosized metallic particle, and dye molecules). The detection method is used to confirm the presence of biomarkers visually and measure absorbance of the colored compounds at a specific wavelength. In this study, we propose a colorimetric assay based on an extended form of double-stranded DNA (dsDNA) self-assembly shielded gold nanoparticles (AuNPs) under positive electrolyte (e.g., 0.1 M MgCl<sub>2</sub>) for detection of Middle East respiratory syndrome coronavirus (MERS-CoV). This platform is able to verify the existence of viral molecules through a localized surface plasmon resonance (LSPR) shift and color changes of AuNPs in the UV-vis wavelength range. We designed a pair of thiol-modified probes at either the 5' end or 3' end to organize complementary base pairs with upstream of the E protein gene (upE) and open reading frames (ORF) 1a on MERS-CoV. The dsDNA of the target and probes forms a disulfide-induced long self-assembled complex, which protects AuNPs from salt-induced aggregation and transition of optical properties. This colorimetric assay could discriminate down to 1 pmol/μL of 30 bp MERS-CoV and further be adapted for convenient on-site detection of other infectious diseases, especially in resource-limited settings.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31062580</PMID><DateCompleted><Year>2020</Year><Month>04</Month><Day>16</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2379-3694</ISSN><JournalIssue CitedMedium="Internet"><Volume>4</Volume><Issue>5</Issue><PubDate><Year>2019</Year><Month>05</Month><Day>24</Day></PubDate></JournalIssue><Title>ACS sensors</Title><ISOAbbreviation>ACS Sens</ISOAbbreviation></Journal><ArticleTitle>Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles.</ArticleTitle><Pagination><MedlinePgn>1306-1312</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acssensors.9b00175</ELocationID><Abstract><AbstractText>Worldwide outbreaks of infectious diseases necessitate the development of rapid and accurate diagnostic methods. Colorimetric assays are a representative tool to simply identify the target molecules in specimens through color changes of an indicator (e.g., nanosized metallic particle, and dye molecules). The detection method is used to confirm the presence of biomarkers visually and measure absorbance of the colored compounds at a specific wavelength. In this study, we propose a colorimetric assay based on an extended form of double-stranded DNA (dsDNA) self-assembly shielded gold nanoparticles (AuNPs) under positive electrolyte (e.g., 0.1 M MgCl<sub>2</sub>) for detection of Middle East respiratory syndrome coronavirus (MERS-CoV). This platform is able to verify the existence of viral molecules through a localized surface plasmon resonance (LSPR) shift and color changes of AuNPs in the UV-vis wavelength range. We designed a pair of thiol-modified probes at either the 5' end or 3' end to organize complementary base pairs with upstream of the E protein gene (upE) and open reading frames (ORF) 1a on MERS-CoV. The dsDNA of the target and probes forms a disulfide-induced long self-assembled complex, which protects AuNPs from salt-induced aggregation and transition of optical properties. This colorimetric assay could discriminate down to 1 pmol/μL of 30 bp MERS-CoV and further be adapted for convenient on-site detection of other infectious diseases, especially in resource-limited settings.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Hanbi</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Minseon</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Medical Device Management and Research, SAIHST (Samsung Advanced Institute for Health Sciences & Technology) , Sungkyunkwan University , Seoul , Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hwang</LastName><ForeName>Joonki</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Jin Hwa</ForeName><Initials>JH</Initials></Author><Author ValidYN="Y"><LastName>Chung</LastName><ForeName>Doo-Ryeon</ForeName><Initials>DR</Initials><AffiliationInfo><Affiliation>Asia Pacific Foundation for Infectious Diseases (APFID) , Seoul , Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Kyu-Sung</ForeName><Initials>KS</Initials><AffiliationInfo><Affiliation>Department of Medical Device Management and Research, SAIHST (Samsung Advanced Institute for Health Sciences & Technology) , Sungkyunkwan University , Seoul , Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Minhee</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0003-0330-7828</Identifier><AffiliationInfo><Affiliation>Department of Medical Device Management and Research, SAIHST (Samsung Advanced Institute for Health Sciences & Technology) , Sungkyunkwan University , Seoul , Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>05</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>ACS Sens</MedlineTA><NlmUniqueID>101669031</NlmUniqueID><ISSNLinking>2379-3694</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004220">Disulfides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-57-5</RegistryNumber><NameOfSubstance UI="D006046">Gold</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>ACS Sens. 2019 Sep 27;4(9):2554</RefSource><PMID Version="1">31483133</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D015374" MajorTopicYN="N">Biosensing Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003124" MajorTopicYN="N">Colorimetry</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004220" MajorTopicYN="N">Disulfides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006046" MajorTopicYN="N">Gold</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057230" MajorTopicYN="N">Limit of Detection</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053768" MajorTopicYN="N">Metal Nanoparticles</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016366" MajorTopicYN="N">Open Reading Frames</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Middle East respiratory syndrome coronavirus (MERS-CoV)</Keyword><Keyword MajorTopicYN="Y">colorimetric assay</Keyword><Keyword MajorTopicYN="Y">gold nanoparticle</Keyword><Keyword MajorTopicYN="Y">label-free detection</Keyword><Keyword MajorTopicYN="Y">molecular diagnosis</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>5</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>5</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31062580</ArticleId><ArticleId 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Chung</name><name sortKey="Kang, Minhee" sort="Kang, Minhee" uniqKey="Kang M" first="Minhee" last="Kang">Minhee Kang</name><name sortKey="Lee, Kyu Sung" sort="Lee, Kyu Sung" uniqKey="Lee K" first="Kyu-Sung" last="Lee">Kyu-Sung Lee</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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