Engineering a Novel Antibody-Peptide Bispecific Fusion Protein Against MERS-CoV.
Identifieur interne : 000585 ( PubMed/Checkpoint ); précédent : 000584; suivant : 000586Engineering a Novel Antibody-Peptide Bispecific Fusion Protein Against MERS-CoV.
Auteurs : Lili Wang [République populaire de Chine] ; Jiyan Xu [République populaire de Chine] ; Yu Kong [République populaire de Chine] ; Ruiying Liang [République populaire de Chine] ; Wei Li [États-Unis] ; Jinyao Li [République populaire de Chine] ; Jun Lu [Nouvelle-Zélande] ; Dimiter S. Dimitrov [États-Unis] ; Fei Yu [République populaire de Chine] ; Yanling Wu [République populaire de Chine] ; Tianlei Ying [République populaire de Chine]Source :
- Antibodies (Basel, Switzerland) [ 2073-4468 ] ; 2019.
Abstract
In recent years, tremendous efforts have been made in the engineering of bispecific or multi-specific antibody-based therapeutics by combining two or more functional antigen-recognizing elements into a single construct. However, to the best of our knowledge there has been no reported cases of effective antiviral antibody-peptide bispecific fusion proteins. We previously developed potent fully human monoclonal antibodies and inhibitory peptides against Middle East Respiratory Syndrome Coronavirus (MERS-CoV), a novel coronavirus that causes severe acute respiratory illness with high mortality. Here, we describe the generation of antibody-peptide bispecific fusion proteins, each of which contains an anti-MERS-CoV single-chain antibody m336 (or normal human IgG1 CH3 domain as a control) linked with, or without, a MERS-CoV fusion inhibitory peptide HR2P. We found that one of these fusion proteins, designated as m336 diabody-pep, exhibited more potent inhibitory activity than the antibody or the peptide alone against pseudotyped MERS-CoV infection and MERS-CoV S protein-mediated cell-cell fusion, suggesting its potential to be developed as an effective bispecific immunotherapeutic for clinical use.
DOI: 10.3390/antib8040053
PubMed: 31690009
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:31690009Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Engineering a Novel Antibody-Peptide Bispecific Fusion Protein Against MERS-CoV.</title><author><name sortKey="Wang, Lili" sort="Wang, Lili" uniqKey="Wang L" first="Lili" last="Wang">Lili Wang</name><affiliation wicri:level="1"><nlm:affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. liliwang@fudan.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Jiyan" sort="Xu, Jiyan" uniqKey="Xu J" first="Jiyan" last="Xu">Jiyan Xu</name><affiliation wicri:level="1"><nlm:affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. 13301050116@fudan.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Kong, Yu" sort="Kong, Yu" uniqKey="Kong Y" first="Yu" last="Kong">Yu Kong</name><affiliation wicri:level="1"><nlm:affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. kongyu@fudan.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Liang, Ruiying" sort="Liang, Ruiying" uniqKey="Liang R" first="Ruiying" last="Liang">Ruiying Liang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life and Science, Hebei Agricultural University, Baoding 071001, China. ruiyingliang@outlook.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life and Science, Hebei Agricultural University, Baoding 071001</wicri:regionArea><wicri:noRegion>Baoding 071001</wicri:noRegion></affiliation></author><author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name><affiliation wicri:level="2"><nlm:affiliation>Center for Antibody Therapeutics, University of Pittsburgh Medical School, Pittsburgh, PA 15261, USA. LIWEI171@pitt.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Antibody Therapeutics, University of Pittsburgh Medical School, Pittsburgh, PA 15261</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Li, Jinyao" sort="Li, Jinyao" uniqKey="Li J" first="Jinyao" last="Li">Jinyao Li</name><affiliation wicri:level="1"><nlm:affiliation>Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. ljyxju@xju.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046</wicri:regionArea><wicri:noRegion>Urumqi 830046</wicri:noRegion></affiliation></author><author><name sortKey="Lu, Jun" sort="Lu, Jun" uniqKey="Lu J" first="Jun" last="Lu">Jun Lu</name><affiliation wicri:level="1"><nlm:affiliation>School of Science, and School of Interprofessional Health Studies, Faculty of Health & Environmental Sciences, Auckland University of Technology, Auckland 1142, New Zealand. jun.lu@aut.ac.nz.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>School of Science, and School of Interprofessional Health Studies, Faculty of Health & Environmental Sciences, Auckland University of Technology, Auckland 1142</wicri:regionArea><wicri:noRegion>Auckland 1142</wicri:noRegion></affiliation></author><author><name sortKey="Dimitrov, Dimiter S" sort="Dimitrov, Dimiter S" uniqKey="Dimitrov D" first="Dimiter S" last="Dimitrov">Dimiter S. Dimitrov</name><affiliation wicri:level="2"><nlm:affiliation>Center for Antibody Therapeutics, University of Pittsburgh Medical School, Pittsburgh, PA 15261, USA. dsd116@pitt.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Antibody Therapeutics, University of Pittsburgh Medical School, Pittsburgh, PA 15261</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Yu, Fei" sort="Yu, Fei" uniqKey="Yu F" first="Fei" last="Yu">Fei Yu</name><affiliation wicri:level="1"><nlm:affiliation>College of Life and Science, Hebei Agricultural University, Baoding 071001, China. shmyf@hebau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life and Science, Hebei Agricultural University, Baoding 071001</wicri:regionArea><wicri:noRegion>Baoding 071001</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Yanling" sort="Wu, Yanling" uniqKey="Wu Y" first="Yanling" last="Wu">Yanling Wu</name><affiliation wicri:level="1"><nlm:affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. yanlingwu@fudan.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Ying, Tianlei" sort="Ying, Tianlei" uniqKey="Ying T" first="Tianlei" last="Ying">Tianlei Ying</name><affiliation wicri:level="1"><nlm:affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. tlying@fudan.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31690009</idno><idno type="pmid">31690009</idno><idno type="doi">10.3390/antib8040053</idno><idno type="wicri:Area/PubMed/Corpus">000376</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000376</idno><idno type="wicri:Area/PubMed/Curation">000376</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000376</idno><idno type="wicri:Area/PubMed/Checkpoint">000585</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000585</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Engineering a Novel Antibody-Peptide Bispecific Fusion Protein Against MERS-CoV.</title><author><name sortKey="Wang, Lili" sort="Wang, Lili" uniqKey="Wang L" first="Lili" last="Wang">Lili Wang</name><affiliation wicri:level="1"><nlm:affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. liliwang@fudan.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Jiyan" sort="Xu, Jiyan" uniqKey="Xu J" first="Jiyan" last="Xu">Jiyan Xu</name><affiliation wicri:level="1"><nlm:affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. 13301050116@fudan.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Kong, Yu" sort="Kong, Yu" uniqKey="Kong Y" first="Yu" last="Kong">Yu Kong</name><affiliation wicri:level="1"><nlm:affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. kongyu@fudan.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Liang, Ruiying" sort="Liang, Ruiying" uniqKey="Liang R" first="Ruiying" last="Liang">Ruiying Liang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life and Science, Hebei Agricultural University, Baoding 071001, China. ruiyingliang@outlook.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life and Science, Hebei Agricultural University, Baoding 071001</wicri:regionArea><wicri:noRegion>Baoding 071001</wicri:noRegion></affiliation></author><author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name><affiliation wicri:level="2"><nlm:affiliation>Center for Antibody Therapeutics, University of Pittsburgh Medical School, Pittsburgh, PA 15261, USA. LIWEI171@pitt.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Antibody Therapeutics, University of Pittsburgh Medical School, Pittsburgh, PA 15261</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Li, Jinyao" sort="Li, Jinyao" uniqKey="Li J" first="Jinyao" last="Li">Jinyao Li</name><affiliation wicri:level="1"><nlm:affiliation>Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. ljyxju@xju.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046</wicri:regionArea><wicri:noRegion>Urumqi 830046</wicri:noRegion></affiliation></author><author><name sortKey="Lu, Jun" sort="Lu, Jun" uniqKey="Lu J" first="Jun" last="Lu">Jun Lu</name><affiliation wicri:level="1"><nlm:affiliation>School of Science, and School of Interprofessional Health Studies, Faculty of Health & Environmental Sciences, Auckland University of Technology, Auckland 1142, New Zealand. jun.lu@aut.ac.nz.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>School of Science, and School of Interprofessional Health Studies, Faculty of Health & Environmental Sciences, Auckland University of Technology, Auckland 1142</wicri:regionArea><wicri:noRegion>Auckland 1142</wicri:noRegion></affiliation></author><author><name sortKey="Dimitrov, Dimiter S" sort="Dimitrov, Dimiter S" uniqKey="Dimitrov D" first="Dimiter S" last="Dimitrov">Dimiter S. Dimitrov</name><affiliation wicri:level="2"><nlm:affiliation>Center for Antibody Therapeutics, University of Pittsburgh Medical School, Pittsburgh, PA 15261, USA. dsd116@pitt.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Antibody Therapeutics, University of Pittsburgh Medical School, Pittsburgh, PA 15261</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Yu, Fei" sort="Yu, Fei" uniqKey="Yu F" first="Fei" last="Yu">Fei Yu</name><affiliation wicri:level="1"><nlm:affiliation>College of Life and Science, Hebei Agricultural University, Baoding 071001, China. shmyf@hebau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life and Science, Hebei Agricultural University, Baoding 071001</wicri:regionArea><wicri:noRegion>Baoding 071001</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Yanling" sort="Wu, Yanling" uniqKey="Wu Y" first="Yanling" last="Wu">Yanling Wu</name><affiliation wicri:level="1"><nlm:affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. yanlingwu@fudan.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author><author><name sortKey="Ying, Tianlei" sort="Ying, Tianlei" uniqKey="Ying T" first="Tianlei" last="Ying">Tianlei Ying</name><affiliation wicri:level="1"><nlm:affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. tlying@fudan.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032</wicri:regionArea><wicri:noRegion>Shanghai 200032</wicri:noRegion></affiliation></author></analytic><series><title level="j">Antibodies (Basel, Switzerland)</title><idno type="eISSN">2073-4468</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In recent years, tremendous efforts have been made in the engineering of bispecific or multi-specific antibody-based therapeutics by combining two or more functional antigen-recognizing elements into a single construct. However, to the best of our knowledge there has been no reported cases of effective antiviral antibody-peptide bispecific fusion proteins. We previously developed potent fully human monoclonal antibodies and inhibitory peptides against Middle East Respiratory Syndrome Coronavirus (MERS-CoV), a novel coronavirus that causes severe acute respiratory illness with high mortality. Here, we describe the generation of antibody-peptide bispecific fusion proteins, each of which contains an anti-MERS-CoV single-chain antibody m336 (or normal human IgG1 CH3 domain as a control) linked with, or without, a MERS-CoV fusion inhibitory peptide HR2P. We found that one of these fusion proteins, designated as m336 diabody-pep, exhibited more potent inhibitory activity than the antibody or the peptide alone against pseudotyped MERS-CoV infection and MERS-CoV S protein-mediated cell-cell fusion, suggesting its potential to be developed as an effective bispecific immunotherapeutic for clinical use.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31690009</PMID><DateRevised><Year>2020</Year><Month>01</Month><Day>28</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2073-4468</ISSN><JournalIssue CitedMedium="Internet"><Volume>8</Volume><Issue>4</Issue><PubDate><Year>2019</Year><Month>Nov</Month><Day>04</Day></PubDate></JournalIssue><Title>Antibodies (Basel, Switzerland)</Title><ISOAbbreviation>Antibodies (Basel)</ISOAbbreviation></Journal><ArticleTitle>Engineering a Novel Antibody-Peptide Bispecific Fusion Protein Against MERS-CoV.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E53</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/antib8040053</ELocationID><Abstract><AbstractText>In recent years, tremendous efforts have been made in the engineering of bispecific or multi-specific antibody-based therapeutics by combining two or more functional antigen-recognizing elements into a single construct. However, to the best of our knowledge there has been no reported cases of effective antiviral antibody-peptide bispecific fusion proteins. We previously developed potent fully human monoclonal antibodies and inhibitory peptides against Middle East Respiratory Syndrome Coronavirus (MERS-CoV), a novel coronavirus that causes severe acute respiratory illness with high mortality. Here, we describe the generation of antibody-peptide bispecific fusion proteins, each of which contains an anti-MERS-CoV single-chain antibody m336 (or normal human IgG1 CH3 domain as a control) linked with, or without, a MERS-CoV fusion inhibitory peptide HR2P. We found that one of these fusion proteins, designated as m336 diabody-pep, exhibited more potent inhibitory activity than the antibody or the peptide alone against pseudotyped MERS-CoV infection and MERS-CoV S protein-mediated cell-cell fusion, suggesting its potential to be developed as an effective bispecific immunotherapeutic for clinical use.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Lili</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. liliwang@fudan.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Research Center of Chinese Jujube, Hebei Agricultural University, Baoding 071001, China. liliwang@fudan.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Jiyan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. 13301050116@fudan.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kong</LastName><ForeName>Yu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. kongyu@fudan.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Ruiying</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>College of Life and Science, Hebei Agricultural University, Baoding 071001, China. ruiyingliang@outlook.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Wei</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Center for Antibody Therapeutics, University of Pittsburgh Medical School, Pittsburgh, PA 15261, USA. LIWEI171@pitt.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jinyao</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0002-3295-6096</Identifier><AffiliationInfo><Affiliation>Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. ljyxju@xju.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Jun</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0003-0225-6643</Identifier><AffiliationInfo><Affiliation>School of Science, and School of Interprofessional Health Studies, Faculty of Health & Environmental Sciences, Auckland University of Technology, Auckland 1142, New Zealand. jun.lu@aut.ac.nz.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dimitrov</LastName><ForeName>Dimiter S</ForeName><Initials>DS</Initials><AffiliationInfo><Affiliation>Center for Antibody Therapeutics, University of Pittsburgh Medical School, Pittsburgh, PA 15261, USA. dsd116@pitt.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Fei</ForeName><Initials>F</Initials><Identifier Source="ORCID">0000-0001-6332-4326</Identifier><AffiliationInfo><Affiliation>College of Life and Science, Hebei Agricultural University, Baoding 071001, China. shmyf@hebau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Yanling</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. yanlingwu@fudan.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ying</LastName><ForeName>Tianlei</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. tlying@fudan.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>11</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Antibodies (Basel)</MedlineTA><NlmUniqueID>101587489</NlmUniqueID><ISSNLinking>2073-4468</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">MERS-CoV</Keyword><Keyword MajorTopicYN="N">bispecific</Keyword><Keyword MajorTopicYN="N">immunotherapeutics</Keyword><Keyword MajorTopicYN="N">mAbs</Keyword><Keyword MajorTopicYN="N">polypeptides</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>08</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>10</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>10</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>11</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>11</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>11</Month><Day>7</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31690009</ArticleId><ArticleId IdType="pii">antib8040053</ArticleId><ArticleId IdType="doi">10.3390/antib8040053</ArticleId><ArticleId IdType="pmc">PMC6963733</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Antiviral Res. 2017 Jul;143:30-37</Citation><ArticleIdList><ArticleId IdType="pubmed">28389142</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2006 Jan;80(2):891-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16378991</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Emerg Microbes Infect. 2017 Jun 7;6(6):e51</Citation><ArticleIdList><ArticleId IdType="pubmed">28588290</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect Dis. 2015 Dec 15;212(12):1894-903</Citation><ArticleIdList><ArticleId IdType="pubmed">26164863</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2007 Apr;81(8):4012-20</Citation><ArticleIdList><ArticleId IdType="pubmed">17267506</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Emerg Infect Dis. 2013 Mar;19(3):456-9</Citation><ArticleIdList><ArticleId IdType="pubmed">23622767</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 2013 Aug 30;288(35):25154-64</Citation><ArticleIdList><ArticleId IdType="pubmed">23867459</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Infect Disord Drug Targets. 2019;19(3):213-223</Citation><ArticleIdList><ArticleId IdType="pubmed">29952267</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Exp Biol Med (Maywood). 2009 Oct;234(10):1117-27</Citation><ArticleIdList><ArticleId IdType="pubmed">19546349</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochim Biophys Acta. 2014 Nov;1844(11):1977-1982</Citation><ArticleIdList><ArticleId IdType="pubmed">24792384</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>MAbs. 2014;6(5):1201-10</Citation><ArticleIdList><ArticleId IdType="pubmed">25517305</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>MAbs. 2017 Feb/Mar;9(2):182-212</Citation><ArticleIdList><ArticleId IdType="pubmed">28071970</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>AIDS. 2018 Aug 24;32(13):1749-1761</Citation><ArticleIdList><ArticleId IdType="pubmed">29762173</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Lancet Infect Dis. 2013 Oct;13(10):859-66</Citation><ArticleIdList><ArticleId IdType="pubmed">23933067</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Microbiol Mol Biol Rev. 2005 Dec;69(4):635-64</Citation><ArticleIdList><ArticleId IdType="pubmed">16339739</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect. 2012 Dec;65(6):477-89</Citation><ArticleIdList><ArticleId IdType="pubmed">23072791</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nature. 2014 Oct 2;514(7520):47-53</Citation><ArticleIdList><ArticleId IdType="pubmed">25171469</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Microbiol Rev. 2015 Apr;28(2):465-522</Citation><ArticleIdList><ArticleId IdType="pubmed">25810418</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2017 Sep 27;91(20):</Citation><ArticleIdList><ArticleId IdType="pubmed">28794022</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Eur J Cell Biol. 2003 Feb;82(2):53-73</Citation><ArticleIdList><ArticleId IdType="pubmed">12647932</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>MAbs. 2015;7(5):922-30</Citation><ArticleIdList><ArticleId IdType="pubmed">26179052</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Commun. 2014;5:3067</Citation><ArticleIdList><ArticleId IdType="pubmed">24473083</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>PLoS One. 2013 Dec 04;8(12):e81587</Citation><ArticleIdList><ArticleId IdType="pubmed">24324708</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Commun. 2015 Sep 15;6:8223</Citation><ArticleIdList><ArticleId IdType="pubmed">26370782</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell Host Microbe. 2017 Oct 11;22(4):471-483.e5</Citation><ArticleIdList><ArticleId IdType="pubmed">28966056</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Sci Transl Med. 2011 Oct 19;3(105):105ra103</Citation><ArticleIdList><ArticleId IdType="pubmed">22013123</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Sci Rep. 2016 Aug 19;6:31629</Citation><ArticleIdList><ArticleId IdType="pubmed">27538452</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virus Res. 2014 Dec 19;194:200-10</Citation><ArticleIdList><ArticleId IdType="pubmed">25451066</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Oncotarget. 2017 Aug 3;8(47):81860-81872</Citation><ArticleIdList><ArticleId IdType="pubmed">29137228</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virol J. 2013 Oct 10;10:304</Citation><ArticleIdList><ArticleId IdType="pubmed">24107353</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Sci Transl Med. 2014 Apr 30;6(234):234ra59</Citation><ArticleIdList><ArticleId IdType="pubmed">24778414</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2014 Jul;88(14):7796-805</Citation><ArticleIdList><ArticleId IdType="pubmed">24789777</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Viruses. 2019 Jan 06;11(1):</Citation><ArticleIdList><ArticleId IdType="pubmed">30621343</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation><ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antiviral Res. 2015 Oct;122:28-38</Citation><ArticleIdList><ArticleId IdType="pubmed">26192750</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect. 2013 May;66(5):464-6</Citation><ArticleIdList><ArticleId IdType="pubmed">23266463</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Viruses. 2019 Aug 01;11(8):null</Citation><ArticleIdList><ArticleId IdType="pubmed">31374953</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect Dis. 2016 May 15;213(10):1557-61</Citation><ArticleIdList><ArticleId IdType="pubmed">26941283</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>PLoS Pathog. 2019 Jun 26;15(6):e1007836</Citation><ArticleIdList><ArticleId IdType="pubmed">31242272</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2014 May 13;111(19):E2018-26</Citation><ArticleIdList><ArticleId IdType="pubmed">24778221</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Front Immunol. 2017 Dec 13;8:1802</Citation><ArticleIdList><ArticleId IdType="pubmed">29326699</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nature. 2013 Mar 14;495(7440):251-4</Citation><ArticleIdList><ArticleId IdType="pubmed">23486063</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2014 Jun;88(12):7045-53</Citation><ArticleIdList><ArticleId IdType="pubmed">24719424</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Emerg Infect Dis. 2013 Nov;19(11):1819-23</Citation><ArticleIdList><ArticleId IdType="pubmed">24206838</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Lancet Infect Dis. 2013 Sep;13(9):752-61</Citation><ArticleIdList><ArticleId IdType="pubmed">23891402</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Front Med. 2017 Dec;11(4):462-470</Citation><ArticleIdList><ArticleId IdType="pubmed">29159596</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Emerg Microbes Infect. 2012 Aug;1(8):e13</Citation><ArticleIdList><ArticleId IdType="pubmed">26038424</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virol Sin. 2016 Feb;31(1):89-93</Citation><ArticleIdList><ArticleId IdType="pubmed">26810660</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2019 Sep 30;93(20):</Citation><ArticleIdList><ArticleId IdType="pubmed">31375583</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Pathog Dis. 2014 Jul;71(2):121-36</Citation><ArticleIdList><ArticleId IdType="pubmed">24585737</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2014 Jun 26;370(26):2499-505</Citation><ArticleIdList><ArticleId IdType="pubmed">24896817</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Emerg Microbes Infect. 2015 Jun 17;4:e37</Citation><ArticleIdList><ArticleId IdType="pubmed">26954884</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2014 May;88(9):5195-9</Citation><ArticleIdList><ArticleId IdType="pubmed">24574399</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Int J Mol Sci. 2018 Feb 06;19(2):null</Citation><ArticleIdList><ArticleId IdType="pubmed">29415501</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Emerg Microbes Infect. 2016 Dec 21;5(12):e128</Citation><ArticleIdList><ArticleId IdType="pubmed">27999424</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Nouvelle-Zélande</li><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Pennsylvanie</li></region></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Lili" sort="Wang, Lili" uniqKey="Wang L" first="Lili" last="Wang">Lili Wang</name></noRegion><name sortKey="Kong, Yu" sort="Kong, Yu" uniqKey="Kong Y" first="Yu" last="Kong">Yu Kong</name><name sortKey="Li, Jinyao" sort="Li, Jinyao" uniqKey="Li J" first="Jinyao" last="Li">Jinyao Li</name><name sortKey="Liang, Ruiying" sort="Liang, Ruiying" uniqKey="Liang R" first="Ruiying" last="Liang">Ruiying Liang</name><name sortKey="Wu, Yanling" sort="Wu, Yanling" uniqKey="Wu Y" first="Yanling" last="Wu">Yanling Wu</name><name sortKey="Xu, Jiyan" sort="Xu, Jiyan" uniqKey="Xu J" first="Jiyan" last="Xu">Jiyan Xu</name><name sortKey="Ying, Tianlei" sort="Ying, Tianlei" uniqKey="Ying T" first="Tianlei" last="Ying">Tianlei Ying</name><name sortKey="Yu, Fei" sort="Yu, Fei" uniqKey="Yu F" first="Fei" last="Yu">Fei Yu</name></country><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name></region><name sortKey="Dimitrov, Dimiter S" sort="Dimitrov, Dimiter S" uniqKey="Dimitrov D" first="Dimiter S" last="Dimitrov">Dimiter S. Dimitrov</name></country><country name="Nouvelle-Zélande"><noRegion><name sortKey="Lu, Jun" sort="Lu, Jun" uniqKey="Lu J" first="Jun" last="Lu">Jun Lu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000585 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000585 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:31690009
|texte= Engineering a Novel Antibody-Peptide Bispecific Fusion Protein Against MERS-CoV.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:31690009" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |