Entry of Scotophilus Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells.
Identifieur interne : 000582 ( PubMed/Checkpoint ); précédent : 000581; suivant : 000583Entry of Scotophilus Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells.
Auteurs : Yi-Ning Chen [Taïwan] ; Hsiao-Chin Hsu [Taïwan] ; Sheng-Wei Wang [Taïwan] ; Hao-Chiang Lien [Taïwan] ; Hsin-Ti Lu [Taïwan] ; Sheng-Kai Peng [Taïwan]Source :
- Pathogens (Basel, Switzerland) [ 2076-0817 ] ; 2019.
Abstract
Bats are natural reservoirs of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome CoV (MERS-CoV). Scotophilus bat CoV-512 demonstrates potential for cross-species transmission because its viral RNA and specific antibodies have been detected in three bat species of Taiwan. Understanding the cell tropism of Scotophilus bat CoV-512 is the first step for studying the mechanism of cross-species transmission. In this study, a lentivirus-based pseudovirus was produced using the spike (S) protein of Scotophilus bat CoV-512 or SARS-CoV as a surface protein to test the interaction between coronaviral S protein and its cell receptor on 11 different cells. Susceptible cells expressed red fluorescence protein (RFP) after the entry of RFP-bound green fluorescence protein (GFP)-fused S protein of Scotophilus bat CoV-512 (RFP-Sco-S-eGFP) or RFP-SARS-S pseudovirus, and firefly luciferase (FLuc) activity expressed by cells infected with FLuc-Sco-S-eGFP or FLuc-SARS-S pseudovirus was quantified. Scotophilus bat CoV-512 pseudovirus had significantly higher entry efficiencies in Madin Darby dog kidney epithelial cells (MDCK), black flying fox brain cells (Pabr), and rat small intestine epithelial cells (IEC-6). SARS-CoV pseudovirus had significantly higher entry efficiencies in human embryonic kidney epithelial cells (HEK-293T), pig kidney epithelial cells (PK15), and MDCK cells. These findings demonstrated that Scotophilus bat CoV-512 had a broad host range for cross-species transmission like SARS-CoV.
DOI: 10.3390/pathogens8040259
PubMed: 31766704
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wicri:step="PubMed">000582</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Entry of <i>Scotophilus</i> Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells.</title><author><name sortKey="Chen, Yi Ning" sort="Chen, Yi Ning" uniqKey="Chen Y" first="Yi-Ning" last="Chen">Yi-Ning Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020</wicri:regionArea><wicri:noRegion>Taoyuan 32020</wicri:noRegion></affiliation></author><author><name sortKey="Hsu, Hsiao Chin" sort="Hsu, Hsiao Chin" uniqKey="Hsu H" first="Hsiao-Chin" last="Hsu">Hsiao-Chin Hsu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020</wicri:regionArea><wicri:noRegion>Taoyuan 32020</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Sheng Wei" sort="Wang, Sheng Wei" uniqKey="Wang S" first="Sheng-Wei" last="Wang">Sheng-Wei Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020</wicri:regionArea><wicri:noRegion>Taoyuan 32020</wicri:noRegion></affiliation></author><author><name sortKey="Lien, Hao Chiang" sort="Lien, Hao Chiang" uniqKey="Lien H" first="Hao-Chiang" last="Lien">Hao-Chiang Lien</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020</wicri:regionArea><wicri:noRegion>Taoyuan 32020</wicri:noRegion></affiliation></author><author><name sortKey="Lu, Hsin Ti" sort="Lu, Hsin Ti" uniqKey="Lu H" first="Hsin-Ti" last="Lu">Hsin-Ti Lu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020</wicri:regionArea><wicri:noRegion>Taoyuan 32020</wicri:noRegion></affiliation></author><author><name sortKey="Peng, Sheng Kai" sort="Peng, Sheng Kai" uniqKey="Peng S" first="Sheng-Kai" last="Peng">Sheng-Kai Peng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020</wicri:regionArea><wicri:noRegion>Taoyuan 32020</wicri:noRegion></affiliation></author></analytic><series><title level="j">Pathogens (Basel, Switzerland)</title><idno type="ISSN">2076-0817</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bats are natural reservoirs of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome CoV (MERS-CoV). <i>Scotophilus</i> bat CoV-512 demonstrates potential for cross-species transmission because its viral RNA and specific antibodies have been detected in three bat species of Taiwan. Understanding the cell tropism of <i>Scotophilus</i> bat CoV-512 is the first step for studying the mechanism of cross-species transmission. In this study, a lentivirus-based pseudovirus was produced using the spike (S) protein of <i>Scotophilus</i> bat CoV-512 or SARS-CoV as a surface protein to test the interaction between coronaviral S protein and its cell receptor on 11 different cells. Susceptible cells expressed red fluorescence protein (RFP) after the entry of RFP-bound green fluorescence protein (GFP)-fused S protein of <i>Scotophilus</i> bat CoV-512 (RFP-Sco-S-eGFP) or RFP-SARS-S pseudovirus, and firefly luciferase (FLuc) activity expressed by cells infected with FLuc-Sco-S-eGFP or FLuc-SARS-S pseudovirus was quantified. <i>Scotophilus</i> bat CoV-512 pseudovirus had significantly higher entry efficiencies in Madin Darby dog kidney epithelial cells (MDCK), black flying fox brain cells (Pabr), and rat small intestine epithelial cells (IEC-6). SARS-CoV pseudovirus had significantly higher entry efficiencies in human embryonic kidney epithelial cells (HEK-293T), pig kidney epithelial cells (PK15), and MDCK cells. These findings demonstrated that <i>Scotophilus</i> bat CoV-512 had a broad host range for cross-species transmission like SARS-CoV.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31766704</PMID><DateRevised><Year>2020</Year><Month>02</Month><Day>27</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2076-0817</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>4</Issue><PubDate><Year>2019</Year><Month>Nov</Month><Day>22</Day></PubDate></JournalIssue><Title>Pathogens (Basel, Switzerland)</Title><ISOAbbreviation>Pathogens</ISOAbbreviation></Journal><ArticleTitle>Entry of <i>Scotophilus</i> Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E259</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/pathogens8040259</ELocationID><Abstract><AbstractText>Bats are natural reservoirs of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome CoV (MERS-CoV). <i>Scotophilus</i> bat CoV-512 demonstrates potential for cross-species transmission because its viral RNA and specific antibodies have been detected in three bat species of Taiwan. Understanding the cell tropism of <i>Scotophilus</i> bat CoV-512 is the first step for studying the mechanism of cross-species transmission. In this study, a lentivirus-based pseudovirus was produced using the spike (S) protein of <i>Scotophilus</i> bat CoV-512 or SARS-CoV as a surface protein to test the interaction between coronaviral S protein and its cell receptor on 11 different cells. Susceptible cells expressed red fluorescence protein (RFP) after the entry of RFP-bound green fluorescence protein (GFP)-fused S protein of <i>Scotophilus</i> bat CoV-512 (RFP-Sco-S-eGFP) or RFP-SARS-S pseudovirus, and firefly luciferase (FLuc) activity expressed by cells infected with FLuc-Sco-S-eGFP or FLuc-SARS-S pseudovirus was quantified. <i>Scotophilus</i> bat CoV-512 pseudovirus had significantly higher entry efficiencies in Madin Darby dog kidney epithelial cells (MDCK), black flying fox brain cells (Pabr), and rat small intestine epithelial cells (IEC-6). SARS-CoV pseudovirus had significantly higher entry efficiencies in human embryonic kidney epithelial cells (HEK-293T), pig kidney epithelial cells (PK15), and MDCK cells. These findings demonstrated that <i>Scotophilus</i> bat CoV-512 had a broad host range for cross-species transmission like SARS-CoV.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yi-Ning</ForeName><Initials>YN</Initials><Identifier Source="ORCID">0000-0003-4821-8511</Identifier><AffiliationInfo><Affiliation>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hsu</LastName><ForeName>Hsiao-Chin</ForeName><Initials>HC</Initials><AffiliationInfo><Affiliation>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Sheng-Wei</ForeName><Initials>SW</Initials><AffiliationInfo><Affiliation>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lien</LastName><ForeName>Hao-Chiang</ForeName><Initials>HC</Initials><AffiliationInfo><Affiliation>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Hsin-Ti</ForeName><Initials>HT</Initials><AffiliationInfo><Affiliation>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peng</LastName><ForeName>Sheng-Kai</ForeName><Initials>SK</Initials><AffiliationInfo><Affiliation>Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan 32020, Taiwan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>MOST 107-2313-B-033-001</GrantID><Agency>Ministry of Science and Technology, Taiwan</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>11</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Pathogens</MedlineTA><NlmUniqueID>101596317</NlmUniqueID><ISSNLinking>2076-0817</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Scotophilus bat coronavirus-512</Keyword><Keyword MajorTopicYN="N">cell receptor</Keyword><Keyword MajorTopicYN="N">pseudovirus</Keyword><Keyword MajorTopicYN="N">severe acute respiratory syndrome-coronavirus</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>10</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>11</Month><Day>20</Day></PubMedPubDate><PubMedPubDate 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Hsin Ti" uniqKey="Lu H" first="Hsin-Ti" last="Lu">Hsin-Ti Lu</name><name sortKey="Peng, Sheng Kai" sort="Peng, Sheng Kai" uniqKey="Peng S" first="Sheng-Kai" last="Peng">Sheng-Kai Peng</name><name sortKey="Wang, Sheng Wei" sort="Wang, Sheng Wei" uniqKey="Wang S" first="Sheng-Wei" last="Wang">Sheng-Wei Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000582 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000582 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:31766704
|texte= Entry of Scotophilus Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:31766704" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |