MersV1, PubMed, Checkpoint, bibRecord, 000457

Migrating a lab-developed MERS-CoV real-time PCR to 3 "Sample to Result" systems: experiences on optimization and validation.

Identifieur interne : 000457 ( PubMed/Checkpoint ); précédent : 000456; suivant : 000458

Migrating a lab-developed MERS-CoV real-time PCR to 3 "Sample to Result" systems: experiences on optimization and validation.

Auteurs : Glynis Frans [Belgique] ; Kurt Beuselinck [Belgique] ; Bart Peeters [Belgique] ; Marc Van Ranst [Belgique] ; Veroniek Saegeman [Belgique] ; Stefanie Desmet [Belgique] ; Katrien Lagrou [Belgique]

Source :

Diagnostic microbiology and infectious disease [ 1879-0070 ] ; 2019.

RBID : pubmed:30929995

Descripteurs français

KwdFr :
- ARN viral (génétique), Amorces ADN, Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Infections à coronavirus (diagnostic), Réaction de polymérisation en chaine en temps réel (), Réaction de polymérisation en chaine en temps réel (normes), Techniques de laboratoire clinique.
MESH :
- diagnostic : Infections à coronavirus.
- génétique : ARN viral, Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- normes : Réaction de polymérisation en chaine en temps réel.
- Amorces ADN, Réaction de polymérisation en chaine en temps réel, Techniques de laboratoire clinique.

English descriptors

KwdEn :
- Clinical Laboratory Techniques, Coronavirus Infections (diagnosis), DNA Primers, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), RNA, Viral (genetics), Real-Time Polymerase Chain Reaction (methods), Real-Time Polymerase Chain Reaction (standards).
MESH :
- chemical , genetics : RNA, Viral.
- chemical : DNA Primers.
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Real-Time Polymerase Chain Reaction.
- standards : Real-Time Polymerase Chain Reaction.
- Clinical Laboratory Techniques.

Abstract

The goal of the study was to adapt our Middle East respiratory syndrome coronavirus (MERS-CoV) lab-developed test (LDT) to 3 "Sample to Result" (S2R) systems: BD MAX (BD), ELITe InGenius (ELITechGroup), and ARIES (Luminex). The BD MAX and InGenius system allowed use of lab-developed primers and TaqMan probes, while ARIES required conversion to MultiCode primers for melting curve analysis. Each device required ≤1 day of training and assay optimization. No discordant results were noted after analysis of 32 External Quality Control (EQC) samples. On a 10-fold dilution series of a MERS-CoV-positive EQC sample, InGenius obtained the highest detection rate. Laboratory technicians rated the ARIES as the user-friendliest. It also required the least hands-on time. BD MAX had the lowest turnaround time and highest throughput. While each device had distinguishing system properties with associated (dis)advantages, the 3 S2R systems were comparable in terms of assay development and validation.

DOI: 10.1016/j.diagmicrobio.2019.02.006
PubMed: 30929995

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">The goal of the study was to adapt our Middle East respiratory syndrome coronavirus (MERS-CoV) lab-developed test (LDT) to 3 "Sample to Result" (S2R) systems: BD MAX (BD), ELITe InGenius (ELITechGroup), and ARIES (Luminex). The BD MAX and InGenius system allowed use of lab-developed primers and TaqMan probes, while ARIES required conversion to MultiCode primers for melting curve analysis. Each device required ≤1 day of training and assay optimization. No discordant results were noted after analysis of 32 External Quality Control (EQC) samples. On a 10-fold dilution series of a MERS-CoV-positive EQC sample, InGenius obtained the highest detection rate. Laboratory technicians rated the ARIES as the user-friendliest. It also required the least hands-on time. BD MAX had the lowest turnaround time and highest throughput. While each device had distinguishing system properties with associated (dis)advantages, the 3 S2R systems were comparable in terms of assay development and validation.</div>
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<Abstract><AbstractText>The goal of the study was to adapt our Middle East respiratory syndrome coronavirus (MERS-CoV) lab-developed test (LDT) to 3 "Sample to Result" (S2R) systems: BD MAX (BD), ELITe InGenius (ELITechGroup), and ARIES (Luminex). The BD MAX and InGenius system allowed use of lab-developed primers and TaqMan probes, while ARIES required conversion to MultiCode primers for melting curve analysis. Each device required ≤1 day of training and assay optimization. No discordant results were noted after analysis of 32 External Quality Control (EQC) samples. On a 10-fold dilution series of a MERS-CoV-positive EQC sample, InGenius obtained the highest detection rate. Laboratory technicians rated the ARIES as the user-friendliest. It also required the least hands-on time. BD MAX had the lowest turnaround time and highest throughput. While each device had distinguishing system properties with associated (dis)advantages, the 3 S2R systems were comparable in terms of assay development and validation.</AbstractText>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Frans</LastName>
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<AffiliationInfo><Affiliation>Department of Laboratory Medicine, University Hospitals Leuven, Herestraat 49, 3000 Leuven, Belgium. Electronic address: veroniek.saegeman@uzleuven.be.</Affiliation>
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<Keyword MajorTopicYN="N">ELITe InGenius</Keyword>
<Keyword MajorTopicYN="N">Middle East respiratory syndrome coronavirus</Keyword>
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<Month>10</Month>
<Day>16</Day>
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<PubMedPubDate PubStatus="revised"><Year>2019</Year>
<Month>02</Month>
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<ReferenceList><Reference><Citation>J Virol. 2013 Jul;87(14):7790-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23678167</ArticleId>
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<ReferenceList><Reference><Citation>Expert Rev Mol Diagn. 2016;16(3):323-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26689497</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Euro Surveill. 2012 Sep 27;17(39):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23041020</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Am Chem Soc. 2004 Apr 14;126(14):4550-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15070373</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Eur J Clin Microbiol Infect Dis. 2008 Dec;27(12):1177-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18551325</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Clin Microbiol. 2017 Aug;55(8):2431-2438</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28539342</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nucleic Acids Res. 2004 Mar 29;32(6):1937-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15051811</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Clin Virol. 2016 Dec;85:65-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27835760</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>mBio. 2012 Nov 20;3(6):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23170002</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Clin Microbiol. 2014 Jan;52(1):67-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24153118</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2014 Jun 26;370(26):2499-505</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24896817</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Lab Autom. 2014 Oct;19(5):468-73</Citation>
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</ArticleIdList>
</Reference>
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   |texte=   Migrating a lab-developed MERS-CoV real-time PCR to 3 "Sample to Result" systems: experiences on optimization and validation.
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Migrating a lab-developed MERS-CoV real-time PCR to 3 "Sample to Result" systems: experiences on optimization and validation.

Migrating a lab-developed MERS-CoV real-time PCR to 3 "Sample to Result" systems: experiences on optimization and validation.

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