Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection.
Identifieur interne : 000154 ( PubMed/Checkpoint ); précédent : 000153; suivant : 000155Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection.
Auteurs : Carrie D. Nicora [États-Unis] ; Amy C. Sims [États-Unis] ; Kent J. Bloodsworth [États-Unis] ; Young-Mo Kim [États-Unis] ; Ronald J. Moore [États-Unis] ; Jennifer E. Kyle [États-Unis] ; Ernesto S. Nakayasu [États-Unis] ; Thomas O. Metz [États-Unis]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2020.
Abstract
Mass spectrometry (MS)-based, integrated proteomics, metabolomics, and lipidomics (collectively, multi-omics) studies provide a very detailed snapshot of virus-induced changes to the host following infection and can lead to the identification of novel prophylactic and therapeutic targets for preventing or lessening disease severity. Multi-omics studies with Middle East respiratory syndrome coronavirus (MERS-CoV) are challenging as the requirements of biosafety level 3 containment limit the numbers of samples that can be safely managed. To address these issues, the multi-omics sample preparation technique MPLEx (metabolite, protein, and lipid extraction) was developed to partition a single sample into three distinct parts (metabolites, proteins, and lipids) for multi-omics analysis, while simultaneously inactivating MERS-CoV by solubilizing and disrupting the viral envelope and denaturing viral proteins. Here we describe the MPLEx protocol, highlight the step of inactivation, and describe the details of downstream processing, instrumental analysis of the three separate analytes, and their subsequent informatics pipelines.
DOI: 10.1007/978-1-0716-0211-9_14
PubMed: 31883096
Affiliations:
- États-Unis
- Caroline du Nord, Washington (État)
- Chapel Hill (Caroline du Nord)
- Université de Caroline du Nord à Chapel Hill
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Nicora</name><affiliation wicri:level="2"><nlm:affiliation>Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Sims, Amy C" sort="Sims, Amy C" uniqKey="Sims A" first="Amy C" last="Sims">Amy C. Sims</name><affiliation wicri:level="4"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region><settlement type="city">Chapel Hill (Caroline du Nord)</settlement></placeName><orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName></affiliation></author><author><name sortKey="Bloodsworth, Kent J" sort="Bloodsworth, Kent J" uniqKey="Bloodsworth K" first="Kent J" last="Bloodsworth">Kent J. 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Nakayasu</name><affiliation wicri:level="2"><nlm:affiliation>Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Metz, Thomas O" sort="Metz, Thomas O" uniqKey="Metz T" first="Thomas O" last="Metz">Thomas O. Metz</name><affiliation wicri:level="2"><nlm:affiliation>Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. Thomas.metz@pnnl.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author></analytic><series><title level="j">Methods in molecular biology (Clifton, N.J.)</title><idno type="eISSN">1940-6029</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mass spectrometry (MS)-based, integrated proteomics, metabolomics, and lipidomics (collectively, multi-omics) studies provide a very detailed snapshot of virus-induced changes to the host following infection and can lead to the identification of novel prophylactic and therapeutic targets for preventing or lessening disease severity. Multi-omics studies with Middle East respiratory syndrome coronavirus (MERS-CoV) are challenging as the requirements of biosafety level 3 containment limit the numbers of samples that can be safely managed. To address these issues, the multi-omics sample preparation technique MPLEx (metabolite, protein, and lipid extraction) was developed to partition a single sample into three distinct parts (metabolites, proteins, and lipids) for multi-omics analysis, while simultaneously inactivating MERS-CoV by solubilizing and disrupting the viral envelope and denaturing viral proteins. Here we describe the MPLEx protocol, highlight the step of inactivation, and describe the details of downstream processing, instrumental analysis of the three separate analytes, and their subsequent informatics pipelines.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31883096</PMID><DateRevised><Year>2020</Year><Month>04</Month><Day>18</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>2099</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol. Biol.</ISOAbbreviation></Journal><ArticleTitle>Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection.</ArticleTitle><Pagination><MedlinePgn>173-194</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-0716-0211-9_14</ELocationID><Abstract><AbstractText>Mass spectrometry (MS)-based, integrated proteomics, metabolomics, and lipidomics (collectively, multi-omics) studies provide a very detailed snapshot of virus-induced changes to the host following infection and can lead to the identification of novel prophylactic and therapeutic targets for preventing or lessening disease severity. Multi-omics studies with Middle East respiratory syndrome coronavirus (MERS-CoV) are challenging as the requirements of biosafety level 3 containment limit the numbers of samples that can be safely managed. To address these issues, the multi-omics sample preparation technique MPLEx (metabolite, protein, and lipid extraction) was developed to partition a single sample into three distinct parts (metabolites, proteins, and lipids) for multi-omics analysis, while simultaneously inactivating MERS-CoV by solubilizing and disrupting the viral envelope and denaturing viral proteins. Here we describe the MPLEx protocol, highlight the step of inactivation, and describe the details of downstream processing, instrumental analysis of the three separate analytes, and their subsequent informatics pipelines.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nicora</LastName><ForeName>Carrie D</ForeName><Initials>CD</Initials><AffiliationInfo><Affiliation>Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sims</LastName><ForeName>Amy C</ForeName><Initials>AC</Initials><AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bloodsworth</LastName><ForeName>Kent J</ForeName><Initials>KJ</Initials><AffiliationInfo><Affiliation>Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Young-Mo</ForeName><Initials>YM</Initials><AffiliationInfo><Affiliation>Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moore</LastName><ForeName>Ronald J</ForeName><Initials>RJ</Initials><AffiliationInfo><Affiliation>Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kyle</LastName><ForeName>Jennifer E</ForeName><Initials>JE</Initials><AffiliationInfo><Affiliation>Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nakayasu</LastName><ForeName>Ernesto S</ForeName><Initials>ES</Initials><AffiliationInfo><Affiliation>Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Metz</LastName><ForeName>Thomas O</ForeName><Initials>TO</Initials><AffiliationInfo><Affiliation>Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. Thomas.metz@pnnl.gov.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>P41 GM103493</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U19 AI106772</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Lipidomics</Keyword><Keyword 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IdType="pmc">PMC7121680</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Cell Host Microbe. 2017 Dec 13;22(6):817-829.e8</Citation><ArticleIdList><ArticleId IdType="pubmed">29154144</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell Microbiol. 2010 Jun;12(6):844-61</Citation><ArticleIdList><ArticleId IdType="pubmed">20088951</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proteomics. 2011 May;11(10):2019-26</Citation><ArticleIdList><ArticleId IdType="pubmed">21500348</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Analyst. 2017 Jan 26;142(3):442-448</Citation><ArticleIdList><ArticleId IdType="pubmed">28091625</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 1957 May;226(1):497-509</Citation><ArticleIdList><ArticleId IdType="pubmed">13428781</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Gen Virol. 2013 Aug;94(Pt 8):1749-1760</Citation><ArticleIdList><ArticleId IdType="pubmed">23620378</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell Host Microbe. 2016 Feb 10;19(2):254-66</Citation><ArticleIdList><ArticleId IdType="pubmed">26867183</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Rev Microbiol. 2008 May;6(5):363-74</Citation><ArticleIdList><ArticleId IdType="pubmed">18414501</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>mSystems. 2016 May 10;1(3):</Citation><ArticleIdList><ArticleId IdType="pubmed">27822525</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Microbiol. 2016 Nov 28;2:16226</Citation><ArticleIdList><ArticleId IdType="pubmed">27892925</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>mBio. 2011 Mar 01;2(1):e00325-10</Citation><ArticleIdList><ArticleId IdType="pubmed">21285433</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Methods Mol Biol. 2017;1550:339-368</Citation><ArticleIdList><ArticleId IdType="pubmed">28188540</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Curr Opin Microbiol. 2017 Oct;39:7-15</Citation><ArticleIdList><ArticleId IdType="pubmed">28806587</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Viruses. 2010 May;2(5):1055-68</Citation><ArticleIdList><ArticleId IdType="pubmed">21994671</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Signal Transduct Target Ther. 2018 Nov 9;3:30</Citation><ArticleIdList><ArticleId IdType="pubmed">30416760</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Anal Chem. 2009 Dec 15;81(24):10038-48</Citation><ArticleIdList><ArticleId IdType="pubmed">19928838</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>mBio. 2014 May 20;5(3):e01174-14</Citation><ArticleIdList><ArticleId IdType="pubmed">24846384</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Commun. 2014 Oct 31;5:5277</Citation><ArticleIdList><ArticleId IdType="pubmed">25358478</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Anal Chem. 2009 May 1;81(9):3429-39</Citation><ArticleIdList><ArticleId IdType="pubmed">19358599</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2019 Feb 26;116(9):3919-3928</Citation><ArticleIdList><ArticleId IdType="pubmed">30808769</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>PLoS Biol. 2008 Sep 16;6(9):e226</Citation><ArticleIdList><ArticleId IdType="pubmed">18798692</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Med. 2016 Jul 7;22(7):701-5</Citation><ArticleIdList><ArticleId IdType="pubmed">27387881</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Comput Biol Chem. 2008 Jun;32(3):215-7</Citation><ArticleIdList><ArticleId IdType="pubmed">18440872</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Bioinformatics. 2015 Dec 1;31(23):3838-40</Citation><ArticleIdList><ArticleId IdType="pubmed">26243018</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell Microbiol. 2015 Jan;17(1):45-50</Citation><ArticleIdList><ArticleId IdType="pubmed">25287059</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2015 May;479-480:609-18</Citation><ArticleIdList><ArticleId IdType="pubmed">25812764</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):E1012-E1021</Citation><ArticleIdList><ArticleId IdType="pubmed">29339515</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Bioinformatics. 2017 Jun 1;33(11):1744-1746</Citation><ArticleIdList><ArticleId IdType="pubmed">28158427</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>PLoS One. 2013 Jul 25;8(7):e69374</Citation><ArticleIdList><ArticleId IdType="pubmed">23935999</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>mBio. 2013 Aug 06;4(4):</Citation><ArticleIdList><ArticleId IdType="pubmed">23919993</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2010 Jan;84(2):833-46</Citation><ArticleIdList><ArticleId IdType="pubmed">19889777</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2013 Apr;87(7):3885-902</Citation><ArticleIdList><ArticleId IdType="pubmed">23365422</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell. 2012 Mar 16;148(6):1132-44</Citation><ArticleIdList><ArticleId IdType="pubmed">22424225</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>BMC Bioinformatics. 2010 Jul 23;11:395</Citation><ArticleIdList><ArticleId IdType="pubmed">20650010</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li><li>Washington (État)</li></region><settlement><li>Chapel Hill 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|texte= Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection.
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