Evaluation of Phage Display Discovered Peptides as Ligands for Prostate-Specific Membrane Antigen (PSMA)
Identifieur interne : 001081 ( Pmc/Curation ); précédent : 001080; suivant : 001082Evaluation of Phage Display Discovered Peptides as Ligands for Prostate-Specific Membrane Antigen (PSMA)
Auteurs : Duanwen Shen [États-Unis] ; Fei Xie [États-Unis] ; W. Barry Edwards [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
The aim of this study was to identify potential ligands of PSMA suitable for further development as novel PSMA-targeted peptides using phage display technology. The human PSMA protein was immobilized as a target followed by incubation with a 15-mer phage display random peptide library. After one round of prescreening and two rounds of screening, high-stringency screening at the third round of panning was performed to identify the highest affinity binders. Phages which had a specific binding activity to PSMA in human prostate cancer cells were isolated and the DNA corresponding to the 15-mers were sequenced to provide three consensus sequences: GDHSPFT, SHFSVGS and EVPRLSLLAVFL as well as other sequences that did not display consensus. Two of the peptide sequences deduced from DNA sequencing of binding phages, SHSFSVGSGDHSPFT and GRFLTGGTGRLLRIS were labeled with 5-carboxyfluorescein and shown to bind and co-internalize with PSMA on human prostate cancer cells by fluorescence microscopy. The high stringency requirements yielded peptides with affinities KD∼1 µM or greater which are suitable starting points for affinity maturation. While these values were less than anticipated, the high stringency did yield peptide sequences that apparently bound to different surfaces on PSMA. These peptide sequences could be the basis for further development of peptides for prostate cancer tumor imaging and therapy.
Url:
DOI: 10.1371/journal.pone.0068339
PubMed: 23935860
PubMed Central: 3723849
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evaluation of Phage Display Discovered Peptides as Ligands for Prostate-Specific Membrane Antigen (PSMA)</title><author><name sortKey="Shen, Duanwen" sort="Shen, Duanwen" uniqKey="Shen D" first="Duanwen" last="Shen">Duanwen Shen</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Radiology, Washington University, St. Louis, Missouri, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Radiology, Washington University, St. Louis, Missouri</wicri:regionArea></affiliation></author><author><name sortKey="Xie, Fei" sort="Xie, Fei" uniqKey="Xie F" first="Fei" last="Xie">Fei Xie</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Radiology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Radiology, University of Pittsburgh, Pittsburgh, Pennsylvania</wicri:regionArea></affiliation></author><author><name sortKey="Edwards, W Barry" sort="Edwards, W Barry" uniqKey="Edwards W" first="W. 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Barry Edwards</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Radiology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Radiology, University of Pittsburgh, Pittsburgh, Pennsylvania</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23935860</idno><idno type="pmc">3723849</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3723849</idno><idno type="RBID">PMC:3723849</idno><idno type="doi">10.1371/journal.pone.0068339</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">001081</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001081</idno><idno type="wicri:Area/Pmc/Curation">001081</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001081</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Evaluation of Phage Display Discovered Peptides as Ligands for Prostate-Specific Membrane Antigen (PSMA)</title><author><name sortKey="Shen, Duanwen" sort="Shen, Duanwen" uniqKey="Shen D" first="Duanwen" last="Shen">Duanwen Shen</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Radiology, Washington University, St. Louis, Missouri, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Radiology, Washington University, St. Louis, Missouri</wicri:regionArea></affiliation></author><author><name sortKey="Xie, Fei" sort="Xie, Fei" uniqKey="Xie F" first="Fei" last="Xie">Fei Xie</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Radiology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Radiology, University of Pittsburgh, Pittsburgh, Pennsylvania</wicri:regionArea></affiliation></author><author><name sortKey="Edwards, W Barry" sort="Edwards, W Barry" uniqKey="Edwards W" first="W. Barry" last="Edwards">W. Barry Edwards</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Radiology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Radiology, University of Pittsburgh, Pittsburgh, Pennsylvania</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The aim of this study was to identify potential ligands of PSMA suitable for further development as novel PSMA-targeted peptides using phage display technology. The human PSMA protein was immobilized as a target followed by incubation with a 15-mer phage display random peptide library. After one round of prescreening and two rounds of screening, high-stringency screening at the third round of panning was performed to identify the highest affinity binders. Phages which had a specific binding activity to PSMA in human prostate cancer cells were isolated and the DNA corresponding to the 15-mers were sequenced to provide three consensus sequences: GDHSPFT, SHFSVGS and EVPRLSLLAVFL as well as other sequences that did not display consensus. Two of the peptide sequences deduced from DNA sequencing of binding phages, SHSFSVGSGDHSPFT and GRFLTGGTGRLLRIS were labeled with 5-carboxyfluorescein and shown to bind and co-internalize with PSMA on human prostate cancer cells by fluorescence microscopy. The high stringency requirements yielded peptides with affinities K<sub>D</sub>∼1 µM or greater which are suitable starting points for affinity maturation. While these values were less than anticipated, the high stringency did yield peptide sequences that apparently bound to different surfaces on PSMA. These peptide sequences could be the basis for further development of peptides for prostate cancer tumor imaging and therapy.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Nam, Rk" uniqKey="Nam R">RK Nam</name></author><author><name sortKey="Oliver, Tk" uniqKey="Oliver T">TK Oliver</name></author><author><name sortKey="Vickers, Aj" uniqKey="Vickers A">AJ Vickers</name></author><author><name sortKey="Thompson, I" uniqKey="Thompson I">I Thompson</name></author><author><name sortKey="Kantoff, Pw" uniqKey="Kantoff P">PW Kantoff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Basch, E" uniqKey="Basch E">E Basch</name></author><author><name sortKey="Oliver, Tk" uniqKey="Oliver T">TK Oliver</name></author><author><name sortKey="Vickers, A" uniqKey="Vickers A">A Vickers</name></author><author><name sortKey="Thompson, I" uniqKey="Thompson I">I Thompson</name></author><author><name sortKey="Kantoff, P" uniqKey="Kantoff P">P Kantoff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hessels, D" uniqKey="Hessels D">D Hessels</name></author><author><name sortKey="Verhaegh, Gw" uniqKey="Verhaegh G">GW Verhaegh</name></author><author><name sortKey="Schalken, Ja" uniqKey="Schalken J">JA Schalken</name></author><author><name sortKey="Witjes, Ja" uniqKey="Witjes J">JA Witjes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roobol, Mj" uniqKey="Roobol M">MJ Roobol</name></author><author><name sortKey="Kranse, R" uniqKey="Kranse R">R Kranse</name></author><author><name sortKey="De Koning, Hj" uniqKey="De Koning H">HJ de Koning</name></author><author><name sortKey="Schroder, Fh" uniqKey="Schroder F">FH Schroder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thompson, Im" uniqKey="Thompson I">IM Thompson</name></author><author><name sortKey="Pauler, Dk" uniqKey="Pauler D">DK Pauler</name></author><author><name sortKey="Goodman, Pj" uniqKey="Goodman P">PJ Goodman</name></author><author><name sortKey="Tangen, Cm" uniqKey="Tangen C">CM Tangen</name></author><author><name sortKey="Lucia, Ms" uniqKey="Lucia M">MS Lucia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Minner, S" uniqKey="Minner S">S Minner</name></author><author><name sortKey="Wittmer, C" uniqKey="Wittmer C">C Wittmer</name></author><author><name sortKey="Graefen, M" uniqKey="Graefen M">M Graefen</name></author><author><name sortKey="Salomon, G" uniqKey="Salomon G">G Salomon</name></author><author><name sortKey="Steuber, T" uniqKey="Steuber T">T Steuber</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiao, Z" uniqKey="Xiao Z">Z Xiao</name></author><author><name sortKey="Adam, Bl" uniqKey="Adam B">BL Adam</name></author><author><name sortKey="Cazares, Lh" uniqKey="Cazares L">LH Cazares</name></author><author><name sortKey="Clements, Ma" uniqKey="Clements M">MA Clements</name></author><author><name sortKey="Davis, Jw" uniqKey="Davis J">JW Davis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ghosh, A" uniqKey="Ghosh A">A Ghosh</name></author><author><name sortKey="Heston, Wd" uniqKey="Heston W">WD Heston</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morris, Mj" uniqKey="Morris M">MJ Morris</name></author><author><name sortKey="Pandit Taskar, N" uniqKey="Pandit Taskar N">N Pandit-Taskar</name></author><author><name sortKey="Divgi, Cr" uniqKey="Divgi C">CR Divgi</name></author><author><name sortKey="Bender, S" uniqKey="Bender S">S Bender</name></author><author><name sortKey="O Donoghue, Ja" uniqKey="O Donoghue J">JA O'Donoghue</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chang, Ss" uniqKey="Chang S">SS Chang</name></author><author><name sortKey="O Keefe, Ds" uniqKey="O Keefe D">DS O'Keefe</name></author><author><name sortKey="Bacich, Dj" uniqKey="Bacich D">DJ Bacich</name></author><author><name sortKey="Reuter, Ve" uniqKey="Reuter V">VE Reuter</name></author><author><name sortKey="Heston, Wd" uniqKey="Heston W">WD Heston</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, H" uniqKey="Liu H">H Liu</name></author><author><name sortKey="Moy, P" uniqKey="Moy P">P Moy</name></author><author><name sortKey="Kim, S" uniqKey="Kim S">S Kim</name></author><author><name sortKey="Xia, Y" uniqKey="Xia Y">Y Xia</name></author><author><name sortKey="Rajasekaran, A" uniqKey="Rajasekaran A">A Rajasekaran</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morris, Mj" uniqKey="Morris M">MJ Morris</name></author><author><name sortKey="Divgi, Cr" uniqKey="Divgi C">CR Divgi</name></author><author><name sortKey="Pandit Taskar, N" uniqKey="Pandit Taskar N">N Pandit-Taskar</name></author><author><name sortKey="Batraki, M" uniqKey="Batraki M">M Batraki</name></author><author><name sortKey="Warren, N" uniqKey="Warren N">N Warren</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Milowsky, Mi" uniqKey="Milowsky M">MI Milowsky</name></author><author><name sortKey="Nanus, Dm" uniqKey="Nanus D">DM Nanus</name></author><author><name sortKey="Kostakoglu, L" uniqKey="Kostakoglu L">L Kostakoglu</name></author><author><name sortKey="Vallabhajosula, S" uniqKey="Vallabhajosula S">S Vallabhajosula</name></author><author><name sortKey="Goldsmith, Sj" uniqKey="Goldsmith S">SJ Goldsmith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bander, Nh" uniqKey="Bander N">NH Bander</name></author><author><name sortKey="Milowsky, Mi" uniqKey="Milowsky M">MI Milowsky</name></author><author><name sortKey="Nanus, Dm" uniqKey="Nanus D">DM Nanus</name></author><author><name sortKey="Kostakoglu, L" uniqKey="Kostakoglu L">L Kostakoglu</name></author><author><name sortKey="Vallabhajosula, S" uniqKey="Vallabhajosula S">S Vallabhajosula</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, H" uniqKey="Liu H">H Liu</name></author><author><name sortKey="Rajasekaran, Ak" uniqKey="Rajasekaran A">AK Rajasekaran</name></author><author><name sortKey="Moy, P" uniqKey="Moy P">P Moy</name></author><author><name sortKey="Xia, Y" uniqKey="Xia Y">Y Xia</name></author><author><name sortKey="Kim, S" uniqKey="Kim S">S Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sodee, Db" uniqKey="Sodee D">DB Sodee</name></author><author><name sortKey="Faulhaber, Pf" uniqKey="Faulhaber P">PF Faulhaber</name></author><author><name sortKey="Nelson, Ad" uniqKey="Nelson A">AD Nelson</name></author><author><name sortKey="Bakale, G" uniqKey="Bakale G">G Bakale</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Banerjee, Sr" uniqKey="Banerjee S">SR Banerjee</name></author><author><name sortKey="Foss, Ca" uniqKey="Foss C">CA Foss</name></author><author><name sortKey="Castanares, M" uniqKey="Castanares M">M Castanares</name></author><author><name sortKey="Mease, Rc" uniqKey="Mease R">RC Mease</name></author><author><name sortKey="Byun, Y" uniqKey="Byun Y">Y Byun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Banerjee, Sr" uniqKey="Banerjee S">SR Banerjee</name></author><author><name sortKey="Pullambhatla, M" uniqKey="Pullambhatla M">M Pullambhatla</name></author><author><name sortKey="Byun, Y" uniqKey="Byun Y">Y Byun</name></author><author><name sortKey="Nimmagadda, S" uniqKey="Nimmagadda S">S Nimmagadda</name></author><author><name sortKey="Green, G" uniqKey="Green G">G Green</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Foss, Ca" uniqKey="Foss C">CA Foss</name></author><author><name sortKey="Byun, Y" uniqKey="Byun Y">Y Byun</name></author><author><name sortKey="Nimmagadda, S" uniqKey="Nimmagadda S">S Nimmagadda</name></author><author><name sortKey="Pullambhatla, M" uniqKey="Pullambhatla M">M Pullambhatla</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Pullambhatla, M" uniqKey="Pullambhatla M">M Pullambhatla</name></author><author><name sortKey="Foss, Ca" uniqKey="Foss C">CA Foss</name></author><author><name sortKey="Byun, Y" uniqKey="Byun Y">Y Byun</name></author><author><name sortKey="Nimmagadda, S" uniqKey="Nimmagadda S">S Nimmagadda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Dhara, S" uniqKey="Dhara S">S Dhara</name></author><author><name sortKey="Banerjee, Sr" uniqKey="Banerjee S">SR Banerjee</name></author><author><name sortKey="Byun, Y" uniqKey="Byun Y">Y Byun</name></author><author><name sortKey="Pullambhatla, M" uniqKey="Pullambhatla M">M Pullambhatla</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Humblet, V" uniqKey="Humblet V">V Humblet</name></author><author><name sortKey="Lapidus, R" uniqKey="Lapidus R">R Lapidus</name></author><author><name sortKey="Williams, Lr" uniqKey="Williams L">LR Williams</name></author><author><name sortKey="Tsukamoto, T" uniqKey="Tsukamoto T">T Tsukamoto</name></author><author><name sortKey="Rojas, C" uniqKey="Rojas C">C Rojas</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Noren, Ka" uniqKey="Noren K">KA Noren</name></author><author><name sortKey="Noren, Cj" uniqKey="Noren C">CJ Noren</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Newton, Jr" uniqKey="Newton J">JR Newton</name></author><author><name sortKey="Kelly, Ka" uniqKey="Kelly K">KA Kelly</name></author><author><name sortKey="Mahmood, U" uniqKey="Mahmood U">U Mahmood</name></author><author><name sortKey="Weissleder, R" uniqKey="Weissleder R">R Weissleder</name></author><author><name sortKey="Deutscher, Sl" uniqKey="Deutscher S">SL Deutscher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Sz" uniqKey="Li S">SZ Li</name></author><author><name sortKey="Mcguire, Mj" uniqKey="Mcguire M">MJ McGuire</name></author><author><name sortKey="Lin, M" uniqKey="Lin M">M Lin</name></author><author><name sortKey="Liu, Yh" uniqKey="Liu Y">YH Liu</name></author><author><name sortKey="Oyama, T" uniqKey="Oyama T">T Oyama</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Shen, D" uniqKey="Shen D">D Shen</name></author><author><name sortKey="Liang, K" uniqKey="Liang K">K Liang</name></author><author><name sortKey="Ye, Y" uniqKey="Ye Y">Y Ye</name></author><author><name sortKey="Tetteh, E" uniqKey="Tetteh E">E Tetteh</name></author><author><name sortKey="Achilefu, S" uniqKey="Achilefu S">S Achilefu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yi, F" uniqKey="Yi F">F Yi</name></author><author><name sortKey="Zhu, P" uniqKey="Zhu P">P Zhu</name></author><author><name sortKey="Southall, N" uniqKey="Southall N">N Southall</name></author><author><name sortKey="Inglese, J" uniqKey="Inglese J">J Inglese</name></author><author><name sortKey="Austin, Cp" uniqKey="Austin C">CP Austin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Quinn, Am" uniqKey="Quinn A">AM Quinn</name></author><author><name sortKey="Bedford, Mt" uniqKey="Bedford M">MT Bedford</name></author><author><name sortKey="Espejo, A" uniqKey="Espejo A">A Espejo</name></author><author><name sortKey="Spannhoff, A" uniqKey="Spannhoff A">A Spannhoff</name></author><author><name sortKey="Austin, Cp" uniqKey="Austin C">CP Austin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wigle, Tj" uniqKey="Wigle T">TJ Wigle</name></author><author><name sortKey="Herold, Jm" uniqKey="Herold J">JM Herold</name></author><author><name sortKey="Senisterra, Ga" uniqKey="Senisterra G">GA Senisterra</name></author><author><name sortKey="Vedadi, M" uniqKey="Vedadi M">M Vedadi</name></author><author><name sortKey="Kireev, Db" uniqKey="Kireev D">DB Kireev</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aggarwal, S" uniqKey="Aggarwal S">S Aggarwal</name></author><author><name sortKey="Singh, P" uniqKey="Singh P">P Singh</name></author><author><name sortKey="Topaloglu, O" uniqKey="Topaloglu O">O Topaloglu</name></author><author><name sortKey="Isaacs, Jt" uniqKey="Isaacs J">JT Isaacs</name></author><author><name sortKey="Denmeade, Sr" uniqKey="Denmeade S">SR Denmeade</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Banerjee, Sr" uniqKey="Banerjee S">SR Banerjee</name></author><author><name sortKey="Pullambhatla, M" uniqKey="Pullambhatla M">M Pullambhatla</name></author><author><name sortKey="Byun, Y" uniqKey="Byun Y">Y Byun</name></author><author><name sortKey="Nimmagadda, S" uniqKey="Nimmagadda S">S Nimmagadda</name></author><author><name sortKey="Foss, Ca" uniqKey="Foss C">CA Foss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, J" uniqKey="Yu J">J Yu</name></author><author><name sortKey="Smith, Gp" uniqKey="Smith G">GP Smith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Davis, Mi" uniqKey="Davis M">MI Davis</name></author><author><name sortKey="Bennett, Mj" uniqKey="Bennett M">MJ Bennett</name></author><author><name sortKey="Thomas, Lm" uniqKey="Thomas L">LM Thomas</name></author><author><name sortKey="Bjorkman, Pj" uniqKey="Bjorkman P">PJ Bjorkman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hillier, Sm" uniqKey="Hillier S">SM Hillier</name></author><author><name sortKey="Maresca, Kp" uniqKey="Maresca K">KP Maresca</name></author><author><name sortKey="Femia, Fj" uniqKey="Femia F">FJ Femia</name></author><author><name sortKey="Marquis, Jc" uniqKey="Marquis J">JC Marquis</name></author><author><name sortKey="Foss, Ca" uniqKey="Foss C">CA Foss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lazar, Ga" uniqKey="Lazar G">GA Lazar</name></author><author><name sortKey="Dang, W" uniqKey="Dang W">W Dang</name></author><author><name sortKey="Karki, S" uniqKey="Karki S">S Karki</name></author><author><name sortKey="Vafa, O" uniqKey="Vafa O">O Vafa</name></author><author><name sortKey="Peng, Js" uniqKey="Peng J">JS Peng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pawlyk, Ac" uniqKey="Pawlyk A">AC Pawlyk</name></author><author><name sortKey="Cassel, Ja" uniqKey="Cassel J">JA Cassel</name></author><author><name sortKey="Blass, Be" uniqKey="Blass B">BE Blass</name></author><author><name sortKey="Reitz, Ab" uniqKey="Reitz A">AB Reitz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mesters, Jr" uniqKey="Mesters J">JR Mesters</name></author><author><name sortKey="Barinka, C" uniqKey="Barinka C">C Barinka</name></author><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author><author><name sortKey="Tsukamoto, T" uniqKey="Tsukamoto T">T Tsukamoto</name></author><author><name sortKey="Majer, P" uniqKey="Majer P">P Majer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arter, Ja" uniqKey="Arter J">JA Arter</name></author><author><name sortKey="Diaz, Je" uniqKey="Diaz J">JE Diaz</name></author><author><name sortKey="Donavan, Kc" uniqKey="Donavan K">KC Donavan</name></author><author><name sortKey="Yuan, T" uniqKey="Yuan T">T Yuan</name></author><author><name sortKey="Penner, Rm" uniqKey="Penner R">RM Penner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lupold, Se" uniqKey="Lupold S">SE Lupold</name></author><author><name sortKey="Rodriguez, R" uniqKey="Rodriguez R">R Rodriguez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, Zy" uniqKey="Zhu Z">ZY Zhu</name></author><author><name sortKey="Zhong, Cp" uniqKey="Zhong C">CP Zhong</name></author><author><name sortKey="Xu, Wf" uniqKey="Xu W">WF Xu</name></author><author><name sortKey="Lin, Gm" uniqKey="Lin G">GM Lin</name></author><author><name sortKey="Ye, Gqw" uniqKey="Ye G">GQW Ye</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Banerjee, Sr" uniqKey="Banerjee S">SR Banerjee</name></author><author><name sortKey="Pullambhatla, M" uniqKey="Pullambhatla M">M Pullambhatla</name></author><author><name sortKey="Shallal, H" uniqKey="Shallal H">H Shallal</name></author><author><name sortKey="Lisok, A" uniqKey="Lisok A">A Lisok</name></author><author><name sortKey="Mease, Rc" uniqKey="Mease R">RC Mease</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Diehnelt, Cw" uniqKey="Diehnelt C">CW Diehnelt</name></author><author><name sortKey="Shah, M" uniqKey="Shah M">M Shah</name></author><author><name sortKey="Gupta, N" uniqKey="Gupta N">N Gupta</name></author><author><name sortKey="Belcher, Pe" uniqKey="Belcher P">PE Belcher</name></author><author><name sortKey="Greving, Mp" uniqKey="Greving M">MP Greving</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, S" uniqKey="Kim S">S Kim</name></author><author><name sortKey="Kim, D" uniqKey="Kim D">D Kim</name></author><author><name sortKey="Jung, Hh" uniqKey="Jung H">HH Jung</name></author><author><name sortKey="Lee, I H" uniqKey="Lee I">I-H Lee</name></author><author><name sortKey="Kim, Jil" uniqKey="Kim J">JIL Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Agnew, Hd" uniqKey="Agnew H">HD Agnew</name></author><author><name sortKey="Rohde, Rd" uniqKey="Rohde R">RD Rohde</name></author><author><name sortKey="Millward, Sw" uniqKey="Millward S">SW Millward</name></author><author><name sortKey="Nag, A" uniqKey="Nag A">A Nag</name></author><author><name sortKey="Yeo, W S" uniqKey="Yeo W">W-S Yeo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lim, H S" uniqKey="Lim H">H-S Lim</name></author><author><name sortKey="Reddy, Mm" uniqKey="Reddy M">MM Reddy</name></author><author><name sortKey="Xiao, X" uniqKey="Xiao X">X Xiao</name></author><author><name sortKey="Wilson, J" uniqKey="Wilson J">J Wilson</name></author><author><name sortKey="Wilson, R" uniqKey="Wilson R">R Wilson</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23935860</article-id><article-id pub-id-type="pmc">3723849</article-id><article-id pub-id-type="publisher-id">PONE-D-13-03639</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0068339</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Biomacromolecule-Ligand Interactions</subject><subject>Chemical Biology</subject><subject>Drug Discovery</subject></subj-group></subj-group><subj-group><subject>Proteomics</subject><subj-group><subject>Synthetic Peptide</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Medicine</subject><subj-group><subject>Drugs and Devices</subject><subj-group><subject>Drug Research and Development</subject></subj-group></subj-group><subj-group><subject>Oncology</subject><subj-group><subject>Oncology Agents</subject></subj-group></subj-group><subj-group><subject>Urology</subject><subj-group><subject>Prostate Diseases</subject><subj-group><subject>Prostate Cancer</subject></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Evaluation of Phage Display Discovered Peptides as Ligands for Prostate-Specific Membrane Antigen (PSMA)</article-title><alt-title alt-title-type="running-head">Discovery of PSMA Peptide-Based Ligands</alt-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Shen</surname><given-names>Duanwen</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Xie</surname><given-names>Fei</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Edwards</surname><given-names>W. Barry</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Radiology, Washington University, St. Louis, Missouri, United States of America</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Radiology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Agoulnik</surname><given-names>Irina U.</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Florida International University, United States of America</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>edwardsw@upmc.edu</email></corresp><fn fn-type="COI-statement"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: DS FX. Performed the experiments: DS FX. Analyzed the data: DS FX. Wrote the paper: DS FX WBE.</p></fn></author-notes><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>25</day><month>7</month><year>2013</year></pub-date><volume>8</volume><issue>7</issue><elocation-id>e68339</elocation-id><history><date date-type="received"><day>24</day><month>1</month><year>2013</year></date><date date-type="accepted"><day>28</day><month>5</month><year>2013</year></date></history><permissions><copyright-statement>© 2013 Shen et al</copyright-statement><copyright-year>2013</copyright-year><copyright-holder>Shen et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.</license-p></license></permissions><abstract><p>The aim of this study was to identify potential ligands of PSMA suitable for further development as novel PSMA-targeted peptides using phage display technology. The human PSMA protein was immobilized as a target followed by incubation with a 15-mer phage display random peptide library. After one round of prescreening and two rounds of screening, high-stringency screening at the third round of panning was performed to identify the highest affinity binders. Phages which had a specific binding activity to PSMA in human prostate cancer cells were isolated and the DNA corresponding to the 15-mers were sequenced to provide three consensus sequences: GDHSPFT, SHFSVGS and EVPRLSLLAVFL as well as other sequences that did not display consensus. Two of the peptide sequences deduced from DNA sequencing of binding phages, SHSFSVGSGDHSPFT and GRFLTGGTGRLLRIS were labeled with 5-carboxyfluorescein and shown to bind and co-internalize with PSMA on human prostate cancer cells by fluorescence microscopy. The high stringency requirements yielded peptides with affinities K<sub>D</sub>∼1 µM or greater which are suitable starting points for affinity maturation. While these values were less than anticipated, the high stringency did yield peptide sequences that apparently bound to different surfaces on PSMA. These peptide sequences could be the basis for further development of peptides for prostate cancer tumor imaging and therapy.</p></abstract><funding-group><funding-statement>This work was funded by the Department of Defense Prostate Cancer Research Program New Investigator Award (W81XWH-08-1-0387, WBE) and a Department of Energy Integrated Research Training Program of Excellence in Radiochemistry (DESC0002032, FX). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="8"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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