The SARS-CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform that Perturbs Membrane Order in a Calcium-Dependent Manner
Identifieur interne : 000E73 ( Pmc/Curation ); précédent : 000E72; suivant : 000E74The SARS-CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform that Perturbs Membrane Order in a Calcium-Dependent Manner
Auteurs : Alex L. Lai [États-Unis] ; Jean K. Millet [États-Unis] ; Susan Daniel [États-Unis] ; Jack H. Freed [États-Unis] ; Gary R. Whittaker [États-Unis]Source :
- Journal of Molecular Biology [ 0022-2836 ] ; 2017.
Abstract
Coronaviruses (CoVs) are a major infectious disease threat and include the pathogenic human pathogens of zoonotic origin: severe acute respiratory syndrome CoV (SARS-CoV) and Middle East respiratory syndrome CoV (MERS-CoV). Entry of CoVs into host cells is mediated by the viral spike (S) protein, which is structurally categorized as a class I viral fusion protein, within the same group as influenza virus and HIV. However, S proteins have two distinct cleavage sites that can be activated by a much wider range of proteases. The exact location of the CoV fusion peptide (FP) has been disputed. However, most evidence suggests that the domain immediately downstream of the S2′ cleavage site is the FP (amino acids 798–818 SFIEDLLFNKVTLADAGFMKQY for SARS-CoV, FP1). In our previous electron spin resonance spectroscopic studies, the membrane-ordering effect of influenza virus, HIV, and Dengue virus FPs has been consistently observed. In this study, we used this effect as a criterion to identify and characterize the
Url:
DOI: 10.1016/j.jmb.2017.10.017
PubMed: 29056462
PubMed Central: 5705393
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000E73
Links to Exploration step
PMC:5705393Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The SARS-CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform that Perturbs Membrane Order in a Calcium-Dependent Manner</title><author><name sortKey="Lai, Alex L" sort="Lai, Alex L" uniqKey="Lai A" first="Alex L." last="Lai">Alex L. Lai</name><affiliation wicri:level="1"><nlm:aff id="af0005">Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853</wicri:regionArea></affiliation></author><author><name sortKey="Millet, Jean K" sort="Millet, Jean K" uniqKey="Millet J" first="Jean K." last="Millet">Jean K. Millet</name><affiliation wicri:level="1"><nlm:aff id="af0010">Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853</wicri:regionArea></affiliation></author><author><name sortKey="Daniel, Susan" sort="Daniel, Susan" uniqKey="Daniel S" first="Susan" last="Daniel">Susan Daniel</name><affiliation wicri:level="1"><nlm:aff id="af0015">School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853</wicri:regionArea></affiliation></author><author><name sortKey="Freed, Jack H" sort="Freed, Jack H" uniqKey="Freed J" first="Jack H." last="Freed">Jack H. Freed</name><affiliation wicri:level="1"><nlm:aff id="af0005">Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853</wicri:regionArea></affiliation></author><author><name sortKey="Whittaker, Gary R" sort="Whittaker, Gary R" uniqKey="Whittaker G" first="Gary R." last="Whittaker">Gary R. Whittaker</name><affiliation wicri:level="1"><nlm:aff id="af0010">Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">29056462</idno><idno type="pmc">5705393</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705393</idno><idno type="RBID">PMC:5705393</idno><idno type="doi">10.1016/j.jmb.2017.10.017</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000E73</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000E73</idno><idno type="wicri:Area/Pmc/Curation">000E73</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000E73</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The SARS-CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform that Perturbs Membrane Order in a Calcium-Dependent Manner</title><author><name sortKey="Lai, Alex L" sort="Lai, Alex L" uniqKey="Lai A" first="Alex L." last="Lai">Alex L. Lai</name><affiliation wicri:level="1"><nlm:aff id="af0005">Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853</wicri:regionArea></affiliation></author><author><name sortKey="Millet, Jean K" sort="Millet, Jean K" uniqKey="Millet J" first="Jean K." last="Millet">Jean K. Millet</name><affiliation wicri:level="1"><nlm:aff id="af0010">Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853</wicri:regionArea></affiliation></author><author><name sortKey="Daniel, Susan" sort="Daniel, Susan" uniqKey="Daniel S" first="Susan" last="Daniel">Susan Daniel</name><affiliation wicri:level="1"><nlm:aff id="af0015">School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853</wicri:regionArea></affiliation></author><author><name sortKey="Freed, Jack H" sort="Freed, Jack H" uniqKey="Freed J" first="Jack H." last="Freed">Jack H. Freed</name><affiliation wicri:level="1"><nlm:aff id="af0005">Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853</wicri:regionArea></affiliation></author><author><name sortKey="Whittaker, Gary R" sort="Whittaker, Gary R" uniqKey="Whittaker G" first="Gary R." last="Whittaker">Gary R. Whittaker</name><affiliation wicri:level="1"><nlm:aff id="af0010">Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of Molecular Biology</title><idno type="ISSN">0022-2836</idno><idno type="eISSN">1089-8638</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Coronaviruses (CoVs) are a major infectious disease threat and include the pathogenic human pathogens of zoonotic origin: severe acute respiratory syndrome CoV (SARS-CoV) and Middle East respiratory syndrome CoV (MERS-CoV). Entry of CoVs into host cells is mediated by the viral spike (S) protein, which is structurally categorized as a class I viral fusion protein, within the same group as influenza virus and HIV. However, S proteins have two distinct cleavage sites that can be activated by a much wider range of proteases. The exact location of the CoV fusion peptide (FP) has been disputed. However, most evidence suggests that the domain immediately downstream of the S2′ cleavage site is the FP (amino acids 798–818 SFIEDLLFNKVTLADAGFMKQY for SARS-CoV, FP1). In our previous electron spin resonance spectroscopic studies, the membrane-ordering effect of influenza virus, HIV, and Dengue virus FPs has been consistently observed. In this study, we used this effect as a criterion to identify and characterize the <italic>bona fide</italic> SARS-CoV FP. Our results indicate that both FP1 and the region immediately downstream (amino acids 816–835 KQYGECLGDINARDLICAQKF, FP2) induce significant membrane ordering. Furthermore, their effects are calcium dependent, which is consistent with <italic>in vivo</italic> data showing that calcium is required for SARS-CoV S-mediated fusion. Isothermal titration calorimetry showed a direct interaction between calcium cations and both FPs. This Ca<sup>2 +</sup>-dependency membrane ordering was not observed with influenza FP, indicating that the CoV FP exhibits a mechanistically different behavior. Membrane-ordering effects are greater and penetrate deeper into membranes when FP1 and FP2 act in a concerted manner, suggesting that they form an extended fusion “platform.”</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="White, J" uniqKey="White J">J. White</name></author><author><name sortKey="Kartenbeck, J" uniqKey="Kartenbeck J">J. Kartenbeck</name></author><author><name sortKey="Helenius, A" uniqKey="Helenius A">A. Helenius</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sieczkarski, S B" uniqKey="Sieczkarski S">S.B. Sieczkarski</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guillen, J" uniqKey="Guillen J">J. Guillén</name></author><author><name sortKey="Perez Berna, A J" uniqKey="Perez Berna A">A.J. Pérez-Berná</name></author><author><name sortKey="Moreno, M R" uniqKey="Moreno M">M.R. Moreno</name></author><author><name sortKey="Villalain, J" uniqKey="Villalain J">J. Villalaín</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guillen, J" uniqKey="Guillen J">J. Guillén</name></author><author><name sortKey="Kinnunen, P K J" uniqKey="Kinnunen P">P.K.J. Kinnunen</name></author><author><name sortKey="Villalain, J" uniqKey="Villalain J">J. Villalaín</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Madu, I G" uniqKey="Madu I">I.G. Madu</name></author><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S. Belouzard</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S. Belouzard</name></author><author><name sortKey="Millet, J K" uniqKey="Millet J">J.K. Millet</name></author><author><name sortKey="Licitra, B N" uniqKey="Licitra B">B.N. Licitra</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sainz, B" uniqKey="Sainz B">B. Sainz</name></author><author><name sortKey="Rausch, J M" uniqKey="Rausch J">J.M. Rausch</name></author><author><name sortKey="Gallaher, W R" uniqKey="Gallaher W">W.R. Gallaher</name></author><author><name sortKey="Garry, R F" uniqKey="Garry R">R.F. Garry</name></author><author><name sortKey="Wimley, W C" uniqKey="Wimley W">W.C. Wimley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lorieau, J L" uniqKey="Lorieau J">J.L. Lorieau</name></author><author><name sortKey="Louis, J M" uniqKey="Louis J">J.M. Louis</name></author><author><name sortKey="Bax, A" uniqKey="Bax A">A. Bax</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Han, X" uniqKey="Han X">X. Han</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Han, X" uniqKey="Han X">X. Han</name></author><author><name sortKey="Bushweller, J H" uniqKey="Bushweller J">J.H. Bushweller</name></author><author><name sortKey="Cafiso, D S" uniqKey="Cafiso D">D.S. Cafiso</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S. Belouzard</name></author><author><name sortKey="Chu, V C" uniqKey="Chu V">V.C. Chu</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Basso, L G M" uniqKey="Basso L">L.G.M. Basso</name></author><author><name sortKey="Vicente, E F" uniqKey="Vicente E">E.F. Vicente</name></author><author><name sortKey="Crusca, E" uniqKey="Crusca E">E. Crusca</name></author><author><name sortKey="Cilli, E M" uniqKey="Cilli E">E.M. Cilli</name></author><author><name sortKey="Costa Filho, A J" uniqKey="Costa Filho A">A.J. Costa-Filho</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mahajan, M" uniqKey="Mahajan M">M. Mahajan</name></author><author><name sortKey="Bhattacharjya, S" uniqKey="Bhattacharjya S">S. Bhattacharjya</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Madu, I G" uniqKey="Madu I">I.G. Madu</name></author><author><name sortKey="Roth, S L" uniqKey="Roth S">S.L. Roth</name></author><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S. Belouzard</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, A C" uniqKey="Walls A">A.C. Walls</name></author><author><name sortKey="Tortorici, M A" uniqKey="Tortorici M">M.A. Tortorici</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Frenz, B" uniqKey="Frenz B">B. Frenz</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J. Rottier</name></author><author><name sortKey="Dimaio, F" uniqKey="Dimaio F">F. DiMaio</name></author><author><name sortKey="Rey, F A" uniqKey="Rey F">F.A. Rey</name></author><author><name sortKey="Veesler, D" uniqKey="Veesler D">D. Veesler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kirchdoerfer, R N" uniqKey="Kirchdoerfer R">R.N. Kirchdoerfer</name></author><author><name sortKey="Cottrell, C A" uniqKey="Cottrell C">C.A. Cottrell</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N. Wang</name></author><author><name sortKey="Pallesen, J" uniqKey="Pallesen J">J. Pallesen</name></author><author><name sortKey="Yassine, H M" uniqKey="Yassine H">H.M. Yassine</name></author><author><name sortKey="Turner, H L" uniqKey="Turner H">H.L. Turner</name></author><author><name sortKey="Corbett, K S" uniqKey="Corbett K">K.S. Corbett</name></author><author><name sortKey="Graham, B S" uniqKey="Graham B">B.S. Graham</name></author><author><name sortKey="Mclellan, J S" uniqKey="Mclellan J">J.S. McLellan</name></author><author><name sortKey="Ward, A B" uniqKey="Ward A">A.B. Ward</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, A C" uniqKey="Walls A">A.C. Walls</name></author><author><name sortKey="Tortorici, M A" uniqKey="Tortorici M">M.A. Tortorici</name></author><author><name sortKey="Frenz, B" uniqKey="Frenz B">B. Frenz</name></author><author><name sortKey="Snijder, J" uniqKey="Snijder J">J. Snijder</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Rey, F A" uniqKey="Rey F">F.A. Rey</name></author><author><name sortKey="Dimaio, F" uniqKey="Dimaio F">F. DiMaio</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.-J. Bosch</name></author><author><name sortKey="Veesler, D" uniqKey="Veesler D">D. Veesler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Panaretakis, T" uniqKey="Panaretakis T">T. Panaretakis</name></author><author><name sortKey="Kepp, O" uniqKey="Kepp O">O. Kepp</name></author><author><name sortKey="Brockmeier, U" uniqKey="Brockmeier U">U. Brockmeier</name></author><author><name sortKey="Tesniere, A" uniqKey="Tesniere A">A. Tesniere</name></author><author><name sortKey="Bjorklund, A C" uniqKey="Bjorklund A">A.C. Bjorklund</name></author><author><name sortKey="Chapman, D C" uniqKey="Chapman D">D.C. Chapman</name></author><author><name sortKey="Durchschlag, M" uniqKey="Durchschlag M">M. Durchschlag</name></author><author><name sortKey="Joza, N" uniqKey="Joza N">N. Joza</name></author><author><name sortKey="Pierron, G" uniqKey="Pierron G">G. Pierron</name></author><author><name sortKey="Van Endert, P" uniqKey="Van Endert P">P. van Endert</name></author><author><name sortKey="Yuan, J" uniqKey="Yuan J">J. Yuan</name></author><author><name sortKey="Zitvogel, L" uniqKey="Zitvogel L">L. Zitvogel</name></author><author><name sortKey="Madeo, F" uniqKey="Madeo F">F. Madeo</name></author><author><name sortKey="Williams, D B" uniqKey="Williams D">D.B. Williams</name></author><author><name sortKey="Kroemer, G" uniqKey="Kroemer G">G. Kroemer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gui, M" uniqKey="Gui M">M. Gui</name></author><author><name sortKey="Song, W" uniqKey="Song W">W. Song</name></author><author><name sortKey="Zhou, H" uniqKey="Zhou H">H. Zhou</name></author><author><name sortKey="Xu, J" uniqKey="Xu J">J. Xu</name></author><author><name sortKey="Chen, S" uniqKey="Chen S">S. Chen</name></author><author><name sortKey="Xiang, Y" uniqKey="Xiang Y">Y. Xiang</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, Y" uniqKey="Yuan Y">Y. Yuan</name></author><author><name sortKey="Cao, D" uniqKey="Cao D">D. Cao</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y. Zhang</name></author><author><name sortKey="Ma, J" uniqKey="Ma J">J. Ma</name></author><author><name sortKey="Qi, J" uniqKey="Qi J">J. Qi</name></author><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q. Wang</name></author><author><name sortKey="Lu, G" uniqKey="Lu G">G. Lu</name></author><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y. Wu</name></author><author><name sortKey="Yan, J" uniqKey="Yan J">J. Yan</name></author><author><name sortKey="Shi, Y" uniqKey="Shi Y">Y. Shi</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X. Zhang</name></author><author><name sortKey="Gao, G F" uniqKey="Gao G">G.F. Gao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, A L" uniqKey="Lai A">A.L. Lai</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, A L" uniqKey="Lai A">A.L. Lai</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pinello, J F" uniqKey="Pinello J">J.F. Pinello</name></author><author><name sortKey="Lai, A L" uniqKey="Lai A">A.L. Lai</name></author><author><name sortKey="Millet, J K" uniqKey="Millet J">J.K. Millet</name></author><author><name sortKey="Cassidy Hanley, D" uniqKey="Cassidy Hanley D">D. Cassidy-Hanley</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author><author><name sortKey="Clark, T G" uniqKey="Clark T">T.G. Clark</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, A L" uniqKey="Lai A">A.L. Lai</name></author><author><name sortKey="Park, H" uniqKey="Park H">H. Park</name></author><author><name sortKey="White, J M" uniqKey="White J">J.M. White</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Han, X" uniqKey="Han X">X. Han</name></author><author><name sortKey="Steinhauer, D A" uniqKey="Steinhauer D">D.A. Steinhauer</name></author><author><name sortKey="Wharton, S A" uniqKey="Wharton S">S.A. Wharton</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Meer, G" uniqKey="Van Meer G">G. van Meer</name></author><author><name sortKey="Voelker, D R" uniqKey="Voelker D">D.R. Voelker</name></author><author><name sortKey="Feigenson, G W" uniqKey="Feigenson G">G.W. Feigenson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, A L" uniqKey="Lai A">A.L. Lai</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author><author><name sortKey="Ellena, J F" uniqKey="Ellena J">J.F. Ellena</name></author><author><name sortKey="Cafiso, D S" uniqKey="Cafiso D">D.S. Cafiso</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takamori, S" uniqKey="Takamori S">S. Takamori</name></author><author><name sortKey="Holt, M" uniqKey="Holt M">M. Holt</name></author><author><name sortKey="Stenius, K" uniqKey="Stenius K">K. Stenius</name></author><author><name sortKey="Lemke, E A" uniqKey="Lemke E">E.A. Lemke</name></author><author><name sortKey="Gronborg, M" uniqKey="Gronborg M">M. Gronborg</name></author><author><name sortKey="Riedel, D" uniqKey="Riedel D">D. Riedel</name></author><author><name sortKey="Urlaub, H" uniqKey="Urlaub H">H. Urlaub</name></author><author><name sortKey="Schenck, S" uniqKey="Schenck S">S. Schenck</name></author><author><name sortKey="Brugger, B" uniqKey="Brugger B">B. Brugger</name></author><author><name sortKey="Ringler, P" uniqKey="Ringler P">P. Ringler</name></author><author><name sortKey="Muller, S A" uniqKey="Muller S">S.A. Muller</name></author><author><name sortKey="Rammner, B" uniqKey="Rammner B">B. Rammner</name></author><author><name sortKey="Grater, F" uniqKey="Grater F">F. Grater</name></author><author><name sortKey="Hub, J S" uniqKey="Hub J">J.S. Hub</name></author><author><name sortKey="De Groot, B L" uniqKey="De Groot B">B.L. De Groot</name></author><author><name sortKey="Mieskes, G" uniqKey="Mieskes G">G. Mieskes</name></author><author><name sortKey="Moriyama, Y" uniqKey="Moriyama Y">Y. Moriyama</name></author><author><name sortKey="Klingauf, J" uniqKey="Klingauf J">J. Klingauf</name></author><author><name sortKey="Grubmuller, H" uniqKey="Grubmuller H">H. Grubmuller</name></author><author><name sortKey="Heuser, J" uniqKey="Heuser J">J. Heuser</name></author><author><name sortKey="Wieland, F" uniqKey="Wieland F">F. Wieland</name></author><author><name sortKey="Jahn, R" uniqKey="Jahn R">R. Jahn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Frazier, A A" uniqKey="Frazier A">A.A. Frazier</name></author><author><name sortKey="Roller, C R" uniqKey="Roller C">C.R. Roller</name></author><author><name sortKey="Havelka, J J" uniqKey="Havelka J">J.J. Havelka</name></author><author><name sortKey="Hinderliter, A" uniqKey="Hinderliter A">A. Hinderliter</name></author><author><name sortKey="Cafiso, D S" uniqKey="Cafiso D">D.S. Cafiso</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Patel, A" uniqKey="Patel A">A. Patel</name></author><author><name sortKey="Mohl, B P" uniqKey="Mohl B">B.-P. Mohl</name></author><author><name sortKey="Roy, P" uniqKey="Roy P">P. Roy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matos, P M" uniqKey="Matos P">P.M. Matos</name></author><author><name sortKey="Marin, M" uniqKey="Marin M">M. Marin</name></author><author><name sortKey="Ahn, B" uniqKey="Ahn B">B. Ahn</name></author><author><name sortKey="Lam, W" uniqKey="Lam W">W. Lam</name></author><author><name sortKey="Santos, N C" uniqKey="Santos N">N.C. Santos</name></author><author><name sortKey="Melikyan, G B" uniqKey="Melikyan G">G.B. Melikyan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaitseva, E" uniqKey="Zaitseva E">E. Zaitseva</name></author><author><name sortKey="Zaitsev, E" uniqKey="Zaitsev E">E. Zaitsev</name></author><author><name sortKey="Melikov, K" uniqKey="Melikov K">K. Melikov</name></author><author><name sortKey="Arakelyan, A" uniqKey="Arakelyan A">A. Arakelyan</name></author><author><name sortKey="Marin, M" uniqKey="Marin M">M. Marin</name></author><author><name sortKey="Villasmil, R" uniqKey="Villasmil R">R. Villasmil</name></author><author><name sortKey="Margolis, L B" uniqKey="Margolis L">L.B. Margolis</name></author><author><name sortKey="Melikyan, G B" uniqKey="Melikyan G">G.B. Melikyan</name></author><author><name sortKey="Chernomordik, L V" uniqKey="Chernomordik L">L.V. Chernomordik</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hu, Y" uniqKey="Hu Y">Y. Hu</name></author><author><name sortKey="Patel, S" uniqKey="Patel S">S. Patel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, R" uniqKey="Yang R">R. Yang</name></author><author><name sortKey="Prorok, M" uniqKey="Prorok M">M. Prorok</name></author><author><name sortKey="Castellino, F J" uniqKey="Castellino F">F.J. Castellino</name></author><author><name sortKey="Weliky, D P" uniqKey="Weliky D">D.P. Weliky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Budil, D E" uniqKey="Budil D">D.E. Budil</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Budil, D E" uniqKey="Budil D">D.E. Budil</name></author><author><name sortKey="Lee, S" uniqKey="Lee S">S. Lee</name></author><author><name sortKey="Saxena, S" uniqKey="Saxena S">S. Saxena</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lou, Y" uniqKey="Lou Y">Y. Lou</name></author><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Freed, J" uniqKey="Freed J">J. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Seelig, J" uniqKey="Seelig J">J. Seelig</name></author><author><name sortKey="Nebel, S" uniqKey="Nebel S">S. Nebel</name></author><author><name sortKey="Ganz, P" uniqKey="Ganz P">P. Ganz</name></author><author><name sortKey="Bruns, C" uniqKey="Bruns C">C. Bruns</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Han, X" uniqKey="Han X">X. Han</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, J" uniqKey="Yang J">J. Yang</name></author><author><name sortKey="Gabrys, C M" uniqKey="Gabrys C">C.M. Gabrys</name></author><author><name sortKey="Weliky, D P" uniqKey="Weliky D">D.P. Weliky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, A L" uniqKey="Lai A">A.L. Lai</name></author><author><name sortKey="Tamm, L K" uniqKey="Tamm L">L.K. Tamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brennan, S C" uniqKey="Brennan S">S.C. Brennan</name></author><author><name sortKey="Wilkinson, W J" uniqKey="Wilkinson W">W.J. Wilkinson</name></author><author><name sortKey="Tseng, H E" uniqKey="Tseng H">H.-E. Tseng</name></author><author><name sortKey="Finney, B" uniqKey="Finney B">B. Finney</name></author><author><name sortKey="Monk, B" uniqKey="Monk B">B. Monk</name></author><author><name sortKey="Dibble, H" uniqKey="Dibble H">H. Dibble</name></author><author><name sortKey="Quilliam, S" uniqKey="Quilliam S">S. Quilliam</name></author><author><name sortKey="Warburton, D" uniqKey="Warburton D">D. Warburton</name></author><author><name sortKey="Galietta, L J" uniqKey="Galietta L">L.J. Galietta</name></author><author><name sortKey="Kemp, P J" uniqKey="Kemp P">P.J. Kemp</name></author><author><name sortKey="Riccardi, D" uniqKey="Riccardi D">D. Riccardi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Millet, J K" uniqKey="Millet J">J.K. Millet</name></author><author><name sortKey="Whittaker, G R" uniqKey="Whittaker G">G.R. Whittaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bhalla, A" uniqKey="Bhalla A">A. Bhalla</name></author><author><name sortKey="Chicka, M C" uniqKey="Chicka M">M.C. Chicka</name></author><author><name sortKey="Tucker, W C" uniqKey="Tucker W">W.C. Tucker</name></author><author><name sortKey="Chapman, E R" uniqKey="Chapman E">E.R. Chapman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brunger, A T" uniqKey="Brunger A">A.T. Brunger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chicka, M C" uniqKey="Chicka M">M.C. Chicka</name></author><author><name sortKey="Hui, E" uniqKey="Hui E">E. Hui</name></author><author><name sortKey="Liu, H" uniqKey="Liu H">H. Liu</name></author><author><name sortKey="Chapman, E R" uniqKey="Chapman E">E.R. Chapman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suarez, T" uniqKey="Suarez T">T. Suarez</name></author><author><name sortKey="Gallaher, W R" uniqKey="Gallaher W">W.R. Gallaher</name></author><author><name sortKey="Agirre, A" uniqKey="Agirre A">A. Agirre</name></author><author><name sortKey="Goni, F M" uniqKey="Goni F">F.M. Goni</name></author><author><name sortKey="Nieva, J L" uniqKey="Nieva J">J.L. Nieva</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dube, M" uniqKey="Dube M">M. Dubé</name></author><author><name sortKey="Rey, F A" uniqKey="Rey F">F.A. Rey</name></author><author><name sortKey="Kielian, M" uniqKey="Kielian M">M. Kielian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Budil, D E" uniqKey="Budil D">D.E. Budil</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Field, K A" uniqKey="Field K">K.A. Field</name></author><author><name sortKey="Aneja, R" uniqKey="Aneja R">R. Aneja</name></author><author><name sortKey="Holowka, D" uniqKey="Holowka D">D. Holowka</name></author><author><name sortKey="Baird, B" uniqKey="Baird B">B. Baird</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, M" uniqKey="Ge M">M. Ge</name></author><author><name sortKey="Gidwani, A" uniqKey="Gidwani A">A. Gidwani</name></author><author><name sortKey="Brown, H A" uniqKey="Brown H">H.A. Brown</name></author><author><name sortKey="Holowka, D" uniqKey="Holowka D">D. Holowka</name></author><author><name sortKey="Baird, B" uniqKey="Baird B">B. Baird</name></author><author><name sortKey="Freed, J H" uniqKey="Freed J">J.H. Freed</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Mol Biol</journal-id><journal-id journal-id-type="iso-abbrev">J. Mol. Biol</journal-id><journal-title-group><journal-title>Journal of Molecular Biology</journal-title></journal-title-group><issn pub-type="ppub">0022-2836</issn><issn pub-type="epub">1089-8638</issn><publisher><publisher-name>Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">29056462</article-id><article-id pub-id-type="pmc">5705393</article-id><article-id pub-id-type="publisher-id">S0022-2836(17)30499-0</article-id><article-id pub-id-type="doi">10.1016/j.jmb.2017.10.017</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>The SARS-CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform that Perturbs Membrane Order in a Calcium-Dependent Manner</article-title></title-group><contrib-group><contrib contrib-type="author" id="au0005"><name><surname>Lai</surname><given-names>Alex L.</given-names></name><xref rid="af0005" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="au0010"><name><surname>Millet</surname><given-names>Jean K.</given-names></name><xref rid="af0010" ref-type="aff">2</xref></contrib><contrib contrib-type="author" id="au0015"><name><surname>Daniel</surname><given-names>Susan</given-names></name><xref rid="af0015" ref-type="aff">3</xref></contrib><contrib contrib-type="author" id="au0020"><name><surname>Freed</surname><given-names>Jack H.</given-names></name><xref rid="af0005" ref-type="aff">1</xref><xref rid="cr0005" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author" id="au0025"><name><surname>Whittaker</surname><given-names>Gary R.</given-names></name><email>gary.whittaker@cornell.edu</email><email>jhf3@cornell.edu</email><xref rid="af0010" ref-type="aff">2</xref><xref rid="cr0005" ref-type="corresp">⁎</xref></contrib></contrib-group><aff id="af0005"><label>1</label>Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, United States</aff><aff id="af0010"><label>2</label>Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, United States</aff><aff id="af0015"><label>3</label>School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, United States</aff><author-notes><corresp id="cr0005"><label>⁎</label>Corresponding authors <email>gary.whittaker@cornell.edu</email><email>jhf3@cornell.edu</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>19</day><month>10</month><year>2017</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>8</day><month>12</month><year>2017</year></pub-date><pub-date pub-type="epub"><day>19</day><month>10</month><year>2017</year></pub-date><volume>429</volume><issue>24</issue><fpage>3875</fpage><lpage>3892</lpage><history><date date-type="received"><day>21</day><month>6</month><year>2017</year></date><date date-type="rev-recd"><day>2</day><month>10</month><year>2017</year></date><date date-type="accepted"><day>12</day><month>10</month><year>2017</year></date></history><permissions><copyright-statement>© 2017 Elsevier Ltd. All rights reserved.</copyright-statement><copyright-year>2017</copyright-year><copyright-holder>Elsevier Ltd</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract id="ab0005"><p>Coronaviruses (CoVs) are a major infectious disease threat and include the pathogenic human pathogens of zoonotic origin: severe acute respiratory syndrome CoV (SARS-CoV) and Middle East respiratory syndrome CoV (MERS-CoV). Entry of CoVs into host cells is mediated by the viral spike (S) protein, which is structurally categorized as a class I viral fusion protein, within the same group as influenza virus and HIV. However, S proteins have two distinct cleavage sites that can be activated by a much wider range of proteases. The exact location of the CoV fusion peptide (FP) has been disputed. However, most evidence suggests that the domain immediately downstream of the S2′ cleavage site is the FP (amino acids 798–818 SFIEDLLFNKVTLADAGFMKQY for SARS-CoV, FP1). In our previous electron spin resonance spectroscopic studies, the membrane-ordering effect of influenza virus, HIV, and Dengue virus FPs has been consistently observed. In this study, we used this effect as a criterion to identify and characterize the <italic>bona fide</italic> SARS-CoV FP. Our results indicate that both FP1 and the region immediately downstream (amino acids 816–835 KQYGECLGDINARDLICAQKF, FP2) induce significant membrane ordering. Furthermore, their effects are calcium dependent, which is consistent with <italic>in vivo</italic> data showing that calcium is required for SARS-CoV S-mediated fusion. Isothermal titration calorimetry showed a direct interaction between calcium cations and both FPs. This Ca<sup>2 +</sup>-dependency membrane ordering was not observed with influenza FP, indicating that the CoV FP exhibits a mechanistically different behavior. Membrane-ordering effects are greater and penetrate deeper into membranes when FP1 and FP2 act in a concerted manner, suggesting that they form an extended fusion “platform.”</p></abstract><abstract abstract-type="graphical" id="ab0010"><title>Graphical Abstract</title><p><fig id="f0045" position="anchor"><alt-text id="al0050">Image 1</alt-text><graphic xlink:href="fx1_lrg"></graphic></fig></p></abstract><abstract abstract-type="author-highlights" id="ab0015"><title>Highlights</title><p><list list-type="simple" id="l0005"><list-item id="li0005"><label>•</label><p id="p0005">ESR and infectivity assays were used to study the SARS-CoV fusion peptide.</p></list-item><list-item id="li0010"><label>•</label><p id="p0010">Membrane-ordering ability was used as a criterion for identifying bona fide FP.</p></list-item><list-item id="li0015"><label>•</label><p id="p0015">FP1 (downstream of S2′ site, aa 798–818) and FP2 (aa 816–835) were identified.</p></list-item><list-item id="li0020"><label>•</label><p id="p0020">FP1 and FP2 act in a cooperative manner to induce greater membrane ordering.</p></list-item><list-item id="li0025"><label>•</label><p id="p0025">FP1- and FP2-mediated membrane fusion is calcium dependent.</p></list-item></list></p></abstract><kwd-group id="ks0005"><title>Keywords</title><kwd>membrane fusion</kwd><kwd>viral entry</kwd><kwd>electron spin resonance</kwd><kwd>coronavirus</kwd><kwd>spike glycoprotein</kwd></kwd-group><kwd-group id="ks0010"><title>Abbreviations</title><kwd>BAPTA-AM, 1,2-bis(2-aminophenoxy)ethane-<italic>N</italic>,<italic>N</italic>,<italic>N</italic>′,<italic>N</italic>′-tetraacetic acid tetrakis</kwd><kwd>CD, circular dichroism</kwd><kwd>Chol, cholesterol</kwd><kwd>CoV, coronavirus</kwd><kwd>ESR, electron spin resonance</kwd><kwd>FP, fusion peptide</kwd><kwd>ITC, isothermal titration calorimetry</kwd><kwd>MERS, Middle East respiratory syndrome</kwd><kwd>MLVs, multilamellar vesicles</kwd><kwd>POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine</kwd><kwd>POPS, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine</kwd><kwd>SARS, severe acute respiratory syndrome</kwd><kwd>SUVs, small unilamellar vesicles</kwd></kwd-group></article-meta><notes><p id="mi0005">Edited by P-Y Lozach</p></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E73 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000E73 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:5705393
|texte= The SARS-CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform that Perturbs Membrane Order in a Calcium-Dependent Manner
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:29056462" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |