MERS-CoV diagnosis: An update
Identifieur interne : 000E39 ( Pmc/Curation ); précédent : 000E38; suivant : 000E40MERS-CoV diagnosis: An update
Auteurs : Sameera Al Johani ; Ali H. HajeerSource :
- Journal of Infection and Public Health [ 1876-0341 ] ; 2016.
Abstract
Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient's testings.
Url:
DOI: 10.1016/j.jiph.2016.04.005
PubMed: 27106390
PubMed Central: 7102781
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">MERS-CoV diagnosis: An update</title><author><name sortKey="Al Johani, Sameera" sort="Al Johani, Sameera" uniqKey="Al Johani S" first="Sameera" last="Al Johani">Sameera Al Johani</name></author><author><name sortKey="Hajeer, Ali H" sort="Hajeer, Ali H" uniqKey="Hajeer A" first="Ali H." last="Hajeer">Ali H. Hajeer</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27106390</idno><idno type="pmc">7102781</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102781</idno><idno type="RBID">PMC:7102781</idno><idno type="doi">10.1016/j.jiph.2016.04.005</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000E39</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000E39</idno><idno type="wicri:Area/Pmc/Curation">000E39</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000E39</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">MERS-CoV diagnosis: An update</title><author><name sortKey="Al Johani, Sameera" sort="Al Johani, Sameera" uniqKey="Al Johani S" first="Sameera" last="Al Johani">Sameera Al Johani</name></author><author><name sortKey="Hajeer, Ali H" sort="Hajeer, Ali H" uniqKey="Hajeer A" first="Ali H." last="Hajeer">Ali H. Hajeer</name></author></analytic><series><title level="j">Journal of Infection and Public Health</title><idno type="ISSN">1876-0341</idno><idno type="eISSN">1876-035X</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Summary</title><p>Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient's testings.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name></author><author><name sortKey="Al Tawfiq, J A" uniqKey="Al Tawfiq J">J.A. Al-Tawfiq</name></author><author><name sortKey="Makhdoom, H Q" uniqKey="Makhdoom H">H.Q. Makhdoom</name></author><author><name sortKey="Assiri, A" uniqKey="Assiri A">A. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Infect Public Health</journal-id><journal-id journal-id-type="iso-abbrev">J Infect Public Health</journal-id><journal-title-group><journal-title>Journal of Infection and Public Health</journal-title></journal-title-group><issn pub-type="ppub">1876-0341</issn><issn pub-type="epub">1876-035X</issn><publisher><publisher-name>Elsevier</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27106390</article-id><article-id pub-id-type="pmc">7102781</article-id><article-id pub-id-type="publisher-id">S1876-0341(16)30022-3</article-id><article-id pub-id-type="doi">10.1016/j.jiph.2016.04.005</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>MERS-CoV diagnosis: An update</article-title></title-group><contrib-group><contrib contrib-type="author" id="aut0005"><name><surname>Al Johani</surname><given-names>Sameera</given-names></name><email>johanis@ngha.med.sa</email><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author" id="aut0010"><name><surname>Hajeer</surname><given-names>Ali H.</given-names></name></contrib></contrib-group><aff id="aff0005">Department of Pathology and Laboratory Medicine, King Saud University for Health Sciences, Riyadh, Saudi Arabia</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author at: Division of Microbiology, Pathology and Laboratory Medicine, Mail Code 1122, King Abdulaziz Medical City, P.O. Box 22490, Riyadh 11426, Saudi Arabia. Tel.: +966 558891414. <email>johanis@ngha.med.sa</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>20</day><month>4</month><year>2016</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><season>May-Jun</season><year>2016</year></pub-date><pub-date pub-type="epub"><day>20</day><month>4</month><year>2016</year></pub-date><volume>9</volume><issue>3</issue><fpage>216</fpage><lpage>219</lpage><history><date date-type="received"><day>4</day><month>4</month><year>2016</year></date><date date-type="accepted"><day>5</day><month>4</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 King Saud Bin Abdulaziz University for Health Sciences. Production and hosting by Elsevier Limited.</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>King Saud Bin Abdulaziz University for Health Sciences</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract id="abs1005"><title>Summary</title><p>Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient's testings.</p></abstract><kwd-group id="kwd0005"><title>Keywords</title><kwd>MERS-COV</kwd><kwd>RT-PCR</kwd><kwd>Serology</kwd><kwd>Sequencing</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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