First international external quality assessment of molecular diagnostics for Mers-CoV
Identifieur interne : 000D96 ( Pmc/Curation ); précédent : 000D95; suivant : 000D97First international external quality assessment of molecular diagnostics for Mers-CoV
Auteurs : Suzan D. Pas [Pays-Bas] ; Pranav Patel [Allemagne] ; Chantal Reusken [Pays-Bas] ; Cristina Domingo [Allemagne] ; Victor M. Corman [Allemagne] ; Christian Drosten [Allemagne] ; Ronald Dijkman [Suisse] ; Volker Thiel [Suisse] ; Norbert Nowotny [Oman, Autriche] ; Marion P. G. Koopmans [Pays-Bas] ; Matthias Niedrig [Allemagne]Source :
- Journal of Clinical Virology [ 1386-6532 ] ; 2015.
Abstract
We describe results of the first HCoV-MERS external quality control panel. 85% of the 189 returned results had a 100% score. 8.1% of laboratories produced false positive results. ORF1b target is not recommended for screenings PCR.
Url:
DOI: 10.1016/j.jcv.2015.05.022
PubMed: 26209385
PubMed Central: 7106520
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Pas</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Patel, Pranav" sort="Patel, Pranav" uniqKey="Patel P" first="Pranav" last="Patel">Pranav Patel</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Domingo, Cristina" sort="Domingo, Cristina" uniqKey="Domingo C" first="Cristina" last="Domingo">Cristina Domingo</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Corman, Victor M" sort="Corman, Victor M" uniqKey="Corman V" first="Victor M." last="Corman">Victor M. Corman</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Institute for virology, University of Bonn, Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for virology, University of Bonn, Bonn</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0020">German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn</wicri:regionArea></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Institute for virology, University of Bonn, Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for virology, University of Bonn, Bonn</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0020">German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn</wicri:regionArea></affiliation></author><author><name sortKey="Dijkman, Ronald" sort="Dijkman, Ronald" uniqKey="Dijkman R" first="Ronald" last="Dijkman">Ronald Dijkman</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Federal Institute of Virology and Immunology, Berne and Mittelhäusern, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Federal Institute of Virology and Immunology, Berne and Mittelhäusern</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0030">Vetsuisse Faculty, University of Berne, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Vetsuisse Faculty, University of Berne</wicri:regionArea></affiliation></author><author><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Federal Institute of Virology and Immunology, Berne and Mittelhäusern, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Federal Institute of Virology and Immunology, Berne and Mittelhäusern</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0030">Vetsuisse Faculty, University of Berne, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Vetsuisse Faculty, University of Berne</wicri:regionArea></affiliation></author><author><name sortKey="Nowotny, Norbert" sort="Nowotny, Norbert" uniqKey="Nowotny N" first="Norbert" last="Nowotny">Norbert Nowotny</name><affiliation wicri:level="1"><nlm:aff id="aff0035">Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman</nlm:aff><country xml:lang="fr">Oman</country><wicri:regionArea>Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0040">Institute of Virology, University of Veterinary Medicine Vienna, Vienna, Austria</nlm:aff><country xml:lang="fr">Autriche</country><wicri:regionArea>Institute of Virology, University of Veterinary Medicine Vienna, Vienna</wicri:regionArea></affiliation></author><author><name sortKey="Koopmans, Marion P G" sort="Koopmans, Marion P G" uniqKey="Koopmans M" first="Marion P. G." last="Koopmans">Marion P. G. Koopmans</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Niedrig, Matthias" sort="Niedrig, Matthias" uniqKey="Niedrig M" first="Matthias" last="Niedrig">Matthias Niedrig</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26209385</idno><idno type="pmc">7106520</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106520</idno><idno type="RBID">PMC:7106520</idno><idno type="doi">10.1016/j.jcv.2015.05.022</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000D96</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000D96</idno><idno type="wicri:Area/Pmc/Curation">000D96</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000D96</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">First international external quality assessment of molecular diagnostics for Mers-CoV</title><author><name sortKey="Pas, Suzan D" sort="Pas, Suzan D" uniqKey="Pas S" first="Suzan D." last="Pas">Suzan D. Pas</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Patel, Pranav" sort="Patel, Pranav" uniqKey="Patel P" first="Pranav" last="Patel">Pranav Patel</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Domingo, Cristina" sort="Domingo, Cristina" uniqKey="Domingo C" first="Cristina" last="Domingo">Cristina Domingo</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Corman, Victor M" sort="Corman, Victor M" uniqKey="Corman V" first="Victor M." last="Corman">Victor M. Corman</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Institute for virology, University of Bonn, Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for virology, University of Bonn, Bonn</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0020">German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn</wicri:regionArea></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Institute for virology, University of Bonn, Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for virology, University of Bonn, Bonn</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0020">German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn</wicri:regionArea></affiliation></author><author><name sortKey="Dijkman, Ronald" sort="Dijkman, Ronald" uniqKey="Dijkman R" first="Ronald" last="Dijkman">Ronald Dijkman</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Federal Institute of Virology and Immunology, Berne and Mittelhäusern, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Federal Institute of Virology and Immunology, Berne and Mittelhäusern</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0030">Vetsuisse Faculty, University of Berne, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Vetsuisse Faculty, University of Berne</wicri:regionArea></affiliation></author><author><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Federal Institute of Virology and Immunology, Berne and Mittelhäusern, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Federal Institute of Virology and Immunology, Berne and Mittelhäusern</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0030">Vetsuisse Faculty, University of Berne, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Vetsuisse Faculty, University of Berne</wicri:regionArea></affiliation></author><author><name sortKey="Nowotny, Norbert" sort="Nowotny, Norbert" uniqKey="Nowotny N" first="Norbert" last="Nowotny">Norbert Nowotny</name><affiliation wicri:level="1"><nlm:aff id="aff0035">Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman</nlm:aff><country xml:lang="fr">Oman</country><wicri:regionArea>Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0040">Institute of Virology, University of Veterinary Medicine Vienna, Vienna, Austria</nlm:aff><country xml:lang="fr">Autriche</country><wicri:regionArea>Institute of Virology, University of Veterinary Medicine Vienna, Vienna</wicri:regionArea></affiliation></author><author><name sortKey="Koopmans, Marion P G" sort="Koopmans, Marion P G" uniqKey="Koopmans M" first="Marion P. G." last="Koopmans">Marion P. G. Koopmans</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Niedrig, Matthias" sort="Niedrig, Matthias" uniqKey="Niedrig M" first="Matthias" last="Niedrig">Matthias Niedrig</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of Clinical Virology</title><idno type="ISSN">1386-6532</idno><idno type="eISSN">1873-5967</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">We describe results of the first HCoV-MERS external quality control panel.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">85% of the 189 returned results had a 100% score.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">8.1% of laboratories produced false positive results.</p></list-item><list-item id="lsti0020"><label>•</label><p id="par0020">ORF1b target is not recommended for screenings PCR.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. Zaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, X" uniqKey="Lu X">X. Lu</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Pereyaslov, D" uniqKey="Pereyaslov D">D. Pereyaslov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pyrc, K" uniqKey="Pyrc K">K. Pyrc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dijkman, R" uniqKey="Dijkman R">R. Dijkman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kapoor, M" uniqKey="Kapoor M">M. Kapoor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kraaij Dirkzwager, M" uniqKey="Kraaij Dirkzwager M">M. Kraaij-Dirkzwager</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Virol</journal-id><journal-id journal-id-type="iso-abbrev">J. Clin. Virol</journal-id><journal-title-group><journal-title>Journal of Clinical Virology</journal-title></journal-title-group><issn pub-type="ppub">1386-6532</issn><issn pub-type="epub">1873-5967</issn><publisher><publisher-name>The Authors. Published by Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26209385</article-id><article-id pub-id-type="pmc">7106520</article-id><article-id pub-id-type="publisher-id">S1386-6532(15)00167-5</article-id><article-id pub-id-type="doi">10.1016/j.jcv.2015.05.022</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>First international external quality assessment of molecular diagnostics for Mers-CoV</article-title></title-group><contrib-group><contrib contrib-type="author" id="aut0005"><name><surname>Pas</surname><given-names>Suzan D.</given-names></name><xref rid="aff0005" ref-type="aff">a</xref><xref rid="cor0010" ref-type="corresp">⁎⁎</xref></contrib><contrib contrib-type="author" id="aut0010"><name><surname>Patel</surname><given-names>Pranav</given-names></name><xref rid="aff0010" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="aut0015"><name><surname>Reusken</surname><given-names>Chantal</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="aut0020"><name><surname>Domingo</surname><given-names>Cristina</given-names></name><xref rid="aff0010" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="aut0025"><name><surname>Corman</surname><given-names>Victor M.</given-names></name><xref rid="aff0015" ref-type="aff">c</xref><xref rid="aff0020" ref-type="aff">d</xref></contrib><contrib contrib-type="author" id="aut0030"><name><surname>Drosten</surname><given-names>Christian</given-names></name><xref rid="aff0015" ref-type="aff">c</xref><xref rid="aff0020" ref-type="aff">d</xref></contrib><contrib contrib-type="author" id="aut0035"><name><surname>Dijkman</surname><given-names>Ronald</given-names></name><xref rid="aff0025" ref-type="aff">e</xref><xref rid="aff0030" ref-type="aff">f</xref></contrib><contrib contrib-type="author" id="aut0040"><name><surname>Thiel</surname><given-names>Volker</given-names></name><xref rid="aff0025" ref-type="aff">e</xref><xref rid="aff0030" ref-type="aff">f</xref></contrib><contrib contrib-type="author" id="aut0045"><name><surname>Nowotny</surname><given-names>Norbert</given-names></name><xref rid="aff0035" ref-type="aff">g</xref><xref rid="aff0040" ref-type="aff">h</xref></contrib><contrib contrib-type="author" id="aut0050"><name><surname>Koopmans</surname><given-names>Marion P.G.</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="aut0055"><name><surname>Niedrig</surname><given-names>Matthias</given-names></name><email>niedrigm@rki.de</email><xref rid="aff0010" ref-type="aff">b</xref><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib></contrib-group><aff id="aff0005"><label>a</label>Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands</aff><aff id="aff0010"><label>b</label>Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany</aff><aff id="aff0015"><label>c</label>Institute for virology, University of Bonn, Bonn, Germany</aff><aff id="aff0020"><label>d</label>German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn, Germany</aff><aff id="aff0025"><label>e</label>Federal Institute of Virology and Immunology, Berne and Mittelhäusern, Switzerland</aff><aff id="aff0030"><label>f</label>Vetsuisse Faculty, University of Berne, Switzerland</aff><aff id="aff0035"><label>g</label>Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman</aff><aff id="aff0040"><label>h</label>Institute of Virology, University of Veterinary Medicine Vienna, Vienna, Austria</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author at: Robert Koch – Institute, Nordufer 20, 13353 Berlin, Germany. Tel.: +49 30 18754 2370; fax: +49 30 18754 2625. <email>niedrigm@rki.de</email></corresp><corresp id="cor0010"><label>⁎⁎</label>Corresponding author at: Department of Viroscience, Erasmus MC, Room Na1017, Wytemaweg 80, 3015CN Rotterdam, The Netherlands.</corresp></author-notes><pub-date pub-type="pmc-release"><day>30</day><month>5</month><year>2015</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>8</month><year>2015</year></pub-date><pub-date pub-type="epub"><day>30</day><month>5</month><year>2015</year></pub-date><volume>69</volume><fpage>81</fpage><lpage>85</lpage><history><date date-type="received"><day>4</day><month>3</month><year>2015</year></date><date date-type="rev-recd"><day>26</day><month>5</month><year>2015</year></date><date date-type="accepted"><day>28</day><month>5</month><year>2015</year></date></history><permissions><copyright-statement>© 2015 The Authors</copyright-statement><copyright-year>2015</copyright-year><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract abstract-type="author-highlights" id="abs0005"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">We describe results of the first HCoV-MERS external quality control panel.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">85% of the 189 returned results had a 100% score.</p></list-item><list-item id="lsti0015"><label>•</label><p id="par0015">8.1% of laboratories produced false positive results.</p></list-item><list-item id="lsti0020"><label>•</label><p id="par0020">ORF1b target is not recommended for screenings PCR.</p></list-item></list></p></abstract><abstract id="abs0010"><sec><title>Background</title><p>Since the discovery of Middle East respiratory syndrome coronavirus (MERS-CoV) in 2012, diagnostic protocols were quickly published and deployed globally.</p></sec><sec><title>Objectives</title><p>We set out to assess the quality of MERS-CoV molecular diagnostics worldwide.</p></sec><sec><title>Study design</title><p>Both sensitivity and specificity were assessed using 12 samples containing different viral loads of MERS-CoV or common coronaviruses (OC43, 229E, NL63, HKU1).</p></sec><sec><title>Results</title><p>The panel was sent to more than 106 participants, of which 99 laboratories from 6 continents returned 189 panel results.Scores ranged from 100% (84 laboratories) to 33% (1 laboratory). 15% of respondents reported quantitative results, 61% semi-quantitative (Ct-values or time to positivity) and 24% reported qualitative results. The major specific technique used was real-time RT-PCR using the WHO recommended targets upE, ORF1a and ORF1b. The evaluation confirmed that RT-PCRs targeting the ORF1b are less sensitive, and therefore not advised for primary diagnostics.</p></sec><sec><title>Conclusions</title><p>The first external quality assessment MERS-CoV panel gives a good insight in molecular diagnostic techniques and their performances for sensitive and specific detection of MERS-CoV RNA globally. Overall, all laboratories were capable of detecting MERS-CoV with some differences in sensitivity. The observation that 8% of laboratories reported false MERS-CoV positive single assay results shows room for improvement, and the importance of using confirmatory targets.</p></sec></abstract><kwd-group id="kwd0005"><title>Keywords</title><kwd>MERS-CoV</kwd><kwd>Real-time RT-PCR</kwd><kwd>EQA</kwd><kwd>QPCR</kwd><kwd>Molecular</kwd><kwd>Diagnosis</kwd><kwd>Viral load</kwd><kwd>Quality control</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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