Genetic Variation between Biomphalaria alexandrina Snails Susceptible and Resistant to Schistosoma mansoni Infection
Identifieur interne : 000B30 ( Pmc/Curation ); précédent : 000B29; suivant : 000B31Genetic Variation between Biomphalaria alexandrina Snails Susceptible and Resistant to Schistosoma mansoni Infection
Auteurs : Suzanne M. F. El-Nassery [Égypte] ; Iman F. Abou-El-Naga [Égypte] ; Sonia R. Allam [Égypte] ; Eman A. Shaat [Égypte] ; Rasha F. M. Mady [Égypte]Source :
- BioMed Research International [ 2314-6133 ] ; 2013.
Abstract
Much effort has been made to control schistosomiasis infection in Egypt. However, enduring effects from such strategies have not yet been achieved. In this study, we sought to determine the genetic variability related to the interaction between
Url:
DOI: 10.1155/2013/160320
PubMed: 23878796
PubMed Central: 3708400
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genetic Variation between <italic>Biomphalaria alexandrina</italic> Snails Susceptible and Resistant to <italic>Schistosoma mansoni</italic> Infection</title><author><name sortKey="El Nassery, Suzanne M F" sort="El Nassery, Suzanne M F" uniqKey="El Nassery S" first="Suzanne M. F." last="El-Nassery">Suzanne M. F. El-Nassery</name><affiliation wicri:level="1"><nlm:aff id="I1">Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria</wicri:regionArea></affiliation></author><author><name sortKey="Abou El Naga, Iman F" sort="Abou El Naga, Iman F" uniqKey="Abou El Naga I" first="Iman F." last="Abou-El-Naga">Iman F. Abou-El-Naga</name><affiliation wicri:level="1"><nlm:aff id="I1">Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria</wicri:regionArea></affiliation></author><author><name sortKey="Allam, Sonia R" sort="Allam, Sonia R" uniqKey="Allam S" first="Sonia R." last="Allam">Sonia R. Allam</name><affiliation wicri:level="1"><nlm:aff id="I1">Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria</wicri:regionArea></affiliation></author><author><name sortKey="Shaat, Eman A" sort="Shaat, Eman A" uniqKey="Shaat E" first="Eman A." last="Shaat">Eman A. Shaat</name><affiliation wicri:level="1"><nlm:aff id="I2">Medical Biochemistry Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Medical Biochemistry Department, Faculty of Medicine, University of Alexandria, Alexandria</wicri:regionArea></affiliation></author><author><name sortKey="Mady, Rasha F M" sort="Mady, Rasha F M" uniqKey="Mady R" first="Rasha F. M." last="Mady">Rasha F. M. Mady</name><affiliation wicri:level="1"><nlm:aff id="I1">Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23878796</idno><idno type="pmc">3708400</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708400</idno><idno type="RBID">PMC:3708400</idno><idno type="doi">10.1155/2013/160320</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000B30</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000B30</idno><idno type="wicri:Area/Pmc/Curation">000B30</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000B30</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Genetic Variation between <italic>Biomphalaria alexandrina</italic> Snails Susceptible and Resistant to <italic>Schistosoma mansoni</italic> Infection</title><author><name sortKey="El Nassery, Suzanne M F" sort="El Nassery, Suzanne M F" uniqKey="El Nassery S" first="Suzanne M. F." last="El-Nassery">Suzanne M. F. El-Nassery</name><affiliation wicri:level="1"><nlm:aff id="I1">Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria</wicri:regionArea></affiliation></author><author><name sortKey="Abou El Naga, Iman F" sort="Abou El Naga, Iman F" uniqKey="Abou El Naga I" first="Iman F." last="Abou-El-Naga">Iman F. Abou-El-Naga</name><affiliation wicri:level="1"><nlm:aff id="I1">Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria</wicri:regionArea></affiliation></author><author><name sortKey="Allam, Sonia R" sort="Allam, Sonia R" uniqKey="Allam S" first="Sonia R." last="Allam">Sonia R. Allam</name><affiliation wicri:level="1"><nlm:aff id="I1">Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria</wicri:regionArea></affiliation></author><author><name sortKey="Shaat, Eman A" sort="Shaat, Eman A" uniqKey="Shaat E" first="Eman A." last="Shaat">Eman A. Shaat</name><affiliation wicri:level="1"><nlm:aff id="I2">Medical Biochemistry Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Medical Biochemistry Department, Faculty of Medicine, University of Alexandria, Alexandria</wicri:regionArea></affiliation></author><author><name sortKey="Mady, Rasha F M" sort="Mady, Rasha F M" uniqKey="Mady R" first="Rasha F. M." last="Mady">Rasha F. M. Mady</name><affiliation wicri:level="1"><nlm:aff id="I1">Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria</wicri:regionArea></affiliation></author></analytic><series><title level="j">BioMed Research International</title><idno type="ISSN">2314-6133</idno><idno type="eISSN">2314-6141</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Much effort has been made to control schistosomiasis infection in Egypt. However, enduring effects from such strategies have not yet been achieved. In this study, we sought to determine the genetic variability related to the interaction between <italic>Biomphalaria alexandrina</italic> snails and <italic>Schistosoma mansoni</italic>. Using RAPD-PCR with eight (10 mers) random primers, we were able to determine the polymorphic markers that differed between snails susceptible and resistant to <italic>Schistosoma mansoni</italic> infection using five primers out of the eight. Our results suggest that the RAPD-PCR technique is an efficient means by which to compare genomes and to detect genetic variations between schistosomiasis intermediate hosts. The RAPD technique with the above-noted primers can identify genomic markers that are specifically related to the <italic>Biomphalaria alexandrina/Schistosoma mansoni</italic> relationship in the absence of specific nucleotide sequence information. This approach could be used in epidemiologic surveys to investigate genetic diversity among <italic>Biomphalaria alexandrina</italic> snails. The ability to determine resistant markers in <italic>Biomphalaria alexandrina</italic> snails could potentially lead to further studies that use refractory snails as agents to control the spread of schistosomiasis.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chitsulo, L" uniqKey="Chitsulo L">L Chitsulo</name></author><author><name sortKey="Engels, D" uniqKey="Engels D">D Engels</name></author><author><name sortKey="Montresor, A" uniqKey="Montresor A">A Montresor</name></author><author><name sortKey="Savioli, L" uniqKey="Savioli L">L Savioli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Steinmann, P" uniqKey="Steinmann P">P Steinmann</name></author><author><name sortKey="Keiser, J" uniqKey="Keiser J">J Keiser</name></author><author><name sortKey="Bos, R" uniqKey="Bos R">R Bos</name></author><author><name sortKey="Tanner, M" uniqKey="Tanner M">M Tanner</name></author><author><name 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Biomed Res Int</journal-id><journal-id journal-id-type="iso-abbrev">Biomed Res Int</journal-id><journal-id journal-id-type="publisher-id">BMRI</journal-id><journal-title-group><journal-title>BioMed Research International</journal-title></journal-title-group><issn pub-type="ppub">2314-6133</issn><issn pub-type="epub">2314-6141</issn><publisher><publisher-name>Hindawi Publishing Corporation</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23878796</article-id><article-id pub-id-type="pmc">3708400</article-id><article-id pub-id-type="doi">10.1155/2013/160320</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Genetic Variation between <italic>Biomphalaria alexandrina</italic> Snails Susceptible and Resistant to <italic>Schistosoma mansoni</italic> Infection</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>El-Nassery</surname><given-names>Suzanne M. F.</given-names></name><xref ref-type="aff" rid="I1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Abou-El-Naga</surname><given-names>Iman F.</given-names></name><xref ref-type="aff" rid="I1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Allam</surname><given-names>Sonia R.</given-names></name><xref ref-type="aff" rid="I1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Shaat</surname><given-names>Eman A.</given-names></name><xref ref-type="aff" rid="I2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Mady</surname><given-names>Rasha F. M.</given-names></name><xref ref-type="aff" rid="I1"><sup>1</sup></xref></contrib></contrib-group><aff id="I1"><sup>1</sup>Medical Parasitology Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt</aff><aff id="I2"><sup>2</sup>Medical Biochemistry Department, Faculty of Medicine, University of Alexandria, Alexandria, Egypt</aff><author-notes><corresp id="cor1">*Iman F. Abou-El-Naga: <email>imanabouelnaga@hotmail.com</email></corresp><fn fn-type="other"><p>Academic Editor: Robert Stoehr</p></fn></author-notes><pub-date pub-type="ppub"><year>2013</year></pub-date><pub-date pub-type="epub"><day>26</day><month>6</month><year>2013</year></pub-date><volume>2013</volume><elocation-id>160320</elocation-id><history><date date-type="received"><day>10</day><month>4</month><year>2013</year></date><date date-type="rev-recd"><day>13</day><month>6</month><year>2013</year></date><date date-type="accepted"><day>15</day><month>6</month><year>2013</year></date></history><permissions><copyright-statement>Copyright © 2013 Suzanne M. F. El-Nassery et al.</copyright-statement><copyright-year>2013</copyright-year><license xlink:href="https://creativecommons.org/licenses/by/3.0/"><license-p>This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><p>Much effort has been made to control schistosomiasis infection in Egypt. However, enduring effects from such strategies have not yet been achieved. In this study, we sought to determine the genetic variability related to the interaction between <italic>Biomphalaria alexandrina</italic> snails and <italic>Schistosoma mansoni</italic>. Using RAPD-PCR with eight (10 mers) random primers, we were able to determine the polymorphic markers that differed between snails susceptible and resistant to <italic>Schistosoma mansoni</italic> infection using five primers out of the eight. Our results suggest that the RAPD-PCR technique is an efficient means by which to compare genomes and to detect genetic variations between schistosomiasis intermediate hosts. The RAPD technique with the above-noted primers can identify genomic markers that are specifically related to the <italic>Biomphalaria alexandrina/Schistosoma mansoni</italic> relationship in the absence of specific nucleotide sequence information. This approach could be used in epidemiologic surveys to investigate genetic diversity among <italic>Biomphalaria alexandrina</italic> snails. The ability to determine resistant markers in <italic>Biomphalaria alexandrina</italic> snails could potentially lead to further studies that use refractory snails as agents to control the spread of schistosomiasis.</p></abstract></article-meta></front><floats-group><fig id="fig1" orientation="portrait" position="float"><label>Figure 1</label><caption><p>Random-amplified PCR of genomic DNA of two different snail strains (resistant and susceptible) of <italic>Biomphalaria alexandrina </italic>with arbitrary primer OPB-18 (5′-CCACAGCAGT-3′). Lane 1: 100 bp DNA ladder and lanes 2–7: individual of resistant snails and lanes 8–13: individual of susceptible <italic>B. alexandrina</italic>. The lanes from 1 to 6 and from 8 to 13 were on the same gel run, but lane 7 was on a separate one.</p></caption><graphic xlink:href="BMRI2013-160320.001"></graphic></fig><fig id="fig2" orientation="portrait" position="float"><label>Figure 2</label><caption><p>Random-amplified PCR of genomic DNA of two different snail strains (resistant and susceptible) of <italic>Biomphalaria alexandrina </italic>with arbitrary primer OPC-11 (5′-AAAGCTGCGG-3′). Lane 1: 100 bp DNA ladder, lanes 2–7; individuals of resistant snails, and lanes 8–13: individuals of susceptible <italic>B. alexandrina</italic>. The lanes from 1 to 13 were on the same gel run, and the space between the marker and lane 2 was wide, so it was cut and the lanes were spliced.</p></caption><graphic xlink:href="BMRI2013-160320.002"></graphic></fig><table-wrap id="tab1" orientation="portrait" position="float"><label>Table 1</label><caption><p>Dice's similarity coefficient (<italic>S</italic>) between susceptible and resistant strains of <italic>Biomphalaria alexandrina </italic>snails.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="1" colspan="1"> </th><th align="center" rowspan="1" colspan="1">OPA-2</th><th align="center" rowspan="1" colspan="1">OPB-18</th><th align="center" rowspan="1" colspan="1">OPC-11</th><th align="center" rowspan="1" colspan="1">OPD-10</th><th align="center" rowspan="1" colspan="1">OPD-18</th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1">Number of shared bands (<italic>a</italic>)</td><td align="center" rowspan="1" colspan="1">2</td><td align="center" rowspan="1" colspan="1">2</td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">2</td><td align="center" rowspan="1" colspan="1">1</td></tr><tr><td align="left" rowspan="1" colspan="1">Number of bands in susceptible not in resistant strain (<italic>b</italic>)</td><td align="center" rowspan="1" colspan="1">1</td><td align="center" rowspan="1" colspan="1">1</td><td align="center" rowspan="1" colspan="1">1</td><td align="center" rowspan="1" colspan="1">1</td><td align="center" rowspan="1" colspan="1">2</td></tr><tr><td align="left" rowspan="1" colspan="1">Number of bands in resistant not in susceptible strain (<italic>c</italic>)</td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">4</td><td align="center" rowspan="1" colspan="1">1</td><td align="center" rowspan="1" colspan="1">3</td></tr><tr><td align="left" rowspan="1" colspan="1">Similarity coefficient (<italic>S</italic>)</td><td align="center" rowspan="1" colspan="1">50%</td><td align="center" rowspan="1" colspan="1">50%</td><td align="center" rowspan="1" colspan="1">55%</td><td align="center" rowspan="1" colspan="1">66%</td><td align="center" rowspan="1" colspan="1">29%</td></tr></tbody></table><table-wrap-foot><fn><p><italic>S</italic> = 2<italic>a</italic>/2<italic>a</italic> + <italic>b</italic> + <italic>c</italic>.</p></fn></table-wrap-foot></table-wrap></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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