Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein
Identifieur interne : 000740 ( Pmc/Curation ); précédent : 000739; suivant : 000741Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein
Auteurs : Ann-Kathrin Haverkamp [Allemagne] ; Berend J. Bosch [Pays-Bas] ; Ingo Spitzbarth [Allemagne] ; Annika Lehmbecker [Allemagne] ; Nigeer Te [Espagne] ; Albert Bensaid [Espagne] ; Joaquim Segalés [Espagne] ; Wolfgang Baumg Rtner [Allemagne]Source :
- Veterinary Immunology and Immunopathology [ 0165-2427 ] ; 2019.
Abstract
Middle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective antibodies which could be used as prophylaxis in prospective human epidemics. In the current study, eight human monoclonal antibodies (mAbs) with neutralizing and non-neutralizing capabilities, directed against different epitopes of the MERS-coronavirus (MERS-CoV) spike (MERS-S) protein, were investigated with regard to their ability to immunohistochemically detect respective epitopes on formalin-fixed paraffin-embedded (FFPE) nasal tissue sections of MERS-CoV experimentally infected alpacas. The most intense immunoreaction was detected using a neutralizing antibody directed against the receptor binding domain S1B of the MERS-S protein, which produced an immunosignal in the cytoplasm of ciliated respiratory epithelium and along the apical membranous region. A similar staining was obtained by two other mAbs which recognize the sialic acid-binding domain and the ectodomain of the membrane fusion subunit S2, respectively. Five mAbs lacked immunoreactivity for MERS-CoV antigen on FFPE tissue, even though they belong, at least in part, to the same epitope group. In summary, three tested human mAbs demonstrated capacity for detection of MERS-CoV antigen on FFPE samples and may be implemented in double or triple immunohistochemical methods.
Url:
DOI: 10.1016/j.vetimm.2019.109939
PubMed: 31526954
PubMed Central: 7112921
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000740
Links to Exploration step
PMC:7112921Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein</title><author><name sortKey="Haverkamp, Ann Kathrin" sort="Haverkamp, Ann Kathrin" uniqKey="Haverkamp A" first="Ann-Kathrin" last="Haverkamp">Ann-Kathrin Haverkamp</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Bosch, Berend J" sort="Bosch, Berend J" uniqKey="Bosch B" first="Berend J." last="Bosch">Berend J. Bosch</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht</wicri:regionArea></affiliation></author><author><name sortKey="Spitzbarth, Ingo" sort="Spitzbarth, Ingo" uniqKey="Spitzbarth I" first="Ingo" last="Spitzbarth">Ingo Spitzbarth</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0015">Center for Systems Neuroscience, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Systems Neuroscience, 30559 Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Lehmbecker, Annika" sort="Lehmbecker, Annika" uniqKey="Lehmbecker A" first="Annika" last="Lehmbecker">Annika Lehmbecker</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0015">Center for Systems Neuroscience, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Systems Neuroscience, 30559 Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Te, Nigeer" sort="Te, Nigeer" uniqKey="Te N" first="Nigeer" last="Te">Nigeer Te</name><affiliation wicri:level="1"><nlm:aff id="aff0020">IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra</wicri:regionArea></affiliation></author><author><name sortKey="Bensaid, Albert" sort="Bensaid, Albert" uniqKey="Bensaid A" first="Albert" last="Bensaid">Albert Bensaid</name><affiliation wicri:level="1"><nlm:aff id="aff0020">IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra</wicri:regionArea></affiliation></author><author><name sortKey="Segales, Joaquim" sort="Segales, Joaquim" uniqKey="Segales J" first="Joaquim" last="Segalés">Joaquim Segalés</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Bellaterra, Barcelona, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Bellaterra, Barcelona</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0030">UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra</wicri:regionArea></affiliation></author><author><name sortKey="Baumg Rtner, Wolfgang" sort="Baumg Rtner, Wolfgang" uniqKey="Baumg Rtner W" first="Wolfgang" last="Baumg Rtner">Wolfgang Baumg Rtner</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0015">Center for Systems Neuroscience, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Systems Neuroscience, 30559 Hannover</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31526954</idno><idno type="pmc">7112921</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112921</idno><idno type="RBID">PMC:7112921</idno><idno type="doi">10.1016/j.vetimm.2019.109939</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000740</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000740</idno><idno type="wicri:Area/Pmc/Curation">000740</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000740</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein</title><author><name sortKey="Haverkamp, Ann Kathrin" sort="Haverkamp, Ann Kathrin" uniqKey="Haverkamp A" first="Ann-Kathrin" last="Haverkamp">Ann-Kathrin Haverkamp</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Bosch, Berend J" sort="Bosch, Berend J" uniqKey="Bosch B" first="Berend J." last="Bosch">Berend J. Bosch</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht</wicri:regionArea></affiliation></author><author><name sortKey="Spitzbarth, Ingo" sort="Spitzbarth, Ingo" uniqKey="Spitzbarth I" first="Ingo" last="Spitzbarth">Ingo Spitzbarth</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0015">Center for Systems Neuroscience, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Systems Neuroscience, 30559 Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Lehmbecker, Annika" sort="Lehmbecker, Annika" uniqKey="Lehmbecker A" first="Annika" last="Lehmbecker">Annika Lehmbecker</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0015">Center for Systems Neuroscience, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Systems Neuroscience, 30559 Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Te, Nigeer" sort="Te, Nigeer" uniqKey="Te N" first="Nigeer" last="Te">Nigeer Te</name><affiliation wicri:level="1"><nlm:aff id="aff0020">IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra</wicri:regionArea></affiliation></author><author><name sortKey="Bensaid, Albert" sort="Bensaid, Albert" uniqKey="Bensaid A" first="Albert" last="Bensaid">Albert Bensaid</name><affiliation wicri:level="1"><nlm:aff id="aff0020">IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra</wicri:regionArea></affiliation></author><author><name sortKey="Segales, Joaquim" sort="Segales, Joaquim" uniqKey="Segales J" first="Joaquim" last="Segalés">Joaquim Segalés</name><affiliation wicri:level="1"><nlm:aff id="aff0025">Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Bellaterra, Barcelona, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Bellaterra, Barcelona</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0030">UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra</wicri:regionArea></affiliation></author><author><name sortKey="Baumg Rtner, Wolfgang" sort="Baumg Rtner, Wolfgang" uniqKey="Baumg Rtner W" first="Wolfgang" last="Baumg Rtner">Wolfgang Baumg Rtner</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0015">Center for Systems Neuroscience, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Systems Neuroscience, 30559 Hannover</wicri:regionArea></affiliation></author></analytic><series><title level="j">Veterinary Immunology and Immunopathology</title><idno type="ISSN">0165-2427</idno><idno type="eISSN">1873-2534</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Middle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective antibodies which could be used as prophylaxis in prospective human epidemics. In the current study, eight human monoclonal antibodies (mAbs) with neutralizing and non-neutralizing capabilities, directed against different epitopes of the MERS-coronavirus (MERS-CoV) spike (MERS-S) protein, were investigated with regard to their ability to immunohistochemically detect respective epitopes on formalin-fixed paraffin-embedded (FFPE) nasal tissue sections of MERS-CoV experimentally infected alpacas. The most intense immunoreaction was detected using a neutralizing antibody directed against the receptor binding domain S1B of the MERS-S protein, which produced an immunosignal in the cytoplasm of ciliated respiratory epithelium and along the apical membranous region. A similar staining was obtained by two other mAbs which recognize the sialic acid-binding domain and the ectodomain of the membrane fusion subunit S2, respectively. Five mAbs lacked immunoreactivity for MERS-CoV antigen on FFPE tissue, even though they belong, at least in part, to the same epitope group. In summary, three tested human mAbs demonstrated capacity for detection of MERS-CoV antigen on FFPE samples and may be implemented in double or triple immunohistochemical methods.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Adney, D R" uniqKey="Adney D">D.R. Adney</name></author><author><name sortKey="Bielefeldt Ohmann, H" uniqKey="Bielefeldt Ohmann H">H. Bielefeldt-Ohmann</name></author><author><name sortKey="Hartwig, A E" uniqKey="Hartwig A">A.E. Hartwig</name></author><author><name sortKey="Bowen, R A" uniqKey="Bowen R">R.A. Bowen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Adney, D R" uniqKey="Adney D">D.R. Adney</name></author><author><name sortKey="Brown, V R" uniqKey="Brown V">V.R. Brown</name></author><author><name sortKey="Porter, S M" uniqKey="Porter S">S.M. Porter</name></author><author><name sortKey="Bielefeldt Ohmann, H" uniqKey="Bielefeldt Ohmann H">H. Bielefeldt-Ohmann</name></author><author><name sortKey="Hartwig, A E" uniqKey="Hartwig A">A.E. Hartwig</name></author><author><name sortKey="Bowen, R A" uniqKey="Bowen R">R.A. Bowen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Adney, D R" uniqKey="Adney D">D.R. Adney</name></author><author><name sortKey="Van Doremalen, N" uniqKey="Van Doremalen N">N. van Doremalen</name></author><author><name sortKey="Brown, V R" uniqKey="Brown V">V.R. Brown</name></author><author><name sortKey="Bushmaker, T" uniqKey="Bushmaker T">T. Bushmaker</name></author><author><name sortKey="Scott, D" uniqKey="Scott D">D. Scott</name></author><author><name sortKey="De Wit, E" uniqKey="De Wit E">E. de Wit</name></author><author><name sortKey="Bowen, R A" uniqKey="Bowen R">R.A. Bowen</name></author><author><name sortKey="Munster, V J" uniqKey="Munster V">V.J. Munster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Agrawal, A S" uniqKey="Agrawal A">A.S. Agrawal</name></author><author><name sortKey="Ying, T" uniqKey="Ying T">T. Ying</name></author><author><name sortKey="Tao, X" uniqKey="Tao X">X. Tao</name></author><author><name sortKey="Garron, T" uniqKey="Garron T">T. Garron</name></author><author><name sortKey="Algaissi, A" uniqKey="Algaissi A">A. Algaissi</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L. Wang</name></author><author><name sortKey="Peng, B H" uniqKey="Peng B">B.H. Peng</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S. Jiang</name></author><author><name sortKey="Dimitrov, D S" uniqKey="Dimitrov D">D.S. Dimitrov</name></author><author><name sortKey="Tseng, C T" uniqKey="Tseng C">C.T. Tseng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Ithete, N L" uniqKey="Ithete N">N.L. Ithete</name></author><author><name sortKey="Richards, L R" uniqKey="Richards L">L.R. Richards</name></author><author><name sortKey="Schoeman, M C" uniqKey="Schoeman M">M.C. Schoeman</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W. Preiser</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Drexler, J F" uniqKey="Drexler J">J.F. Drexler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corti, D" uniqKey="Corti D">D. Corti</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J. Zhao</name></author><author><name sortKey="Pedotti, M" uniqKey="Pedotti M">M. Pedotti</name></author><author><name sortKey="Simonelli, L" uniqKey="Simonelli L">L. Simonelli</name></author><author><name sortKey="Agnihothram, S" uniqKey="Agnihothram S">S. Agnihothram</name></author><author><name sortKey="Fett, C" uniqKey="Fett C">C. Fett</name></author><author><name sortKey="Fernandez Rodriguez, B" uniqKey="Fernandez Rodriguez B">B. Fernandez-Rodriguez</name></author><author><name sortKey="Foglierini, M" uniqKey="Foglierini M">M. Foglierini</name></author><author><name sortKey="Agatic, G" uniqKey="Agatic G">G. Agatic</name></author><author><name sortKey="Vanzetta, F" uniqKey="Vanzetta F">F. Vanzetta</name></author><author><name sortKey="Gopal, R" uniqKey="Gopal R">R. Gopal</name></author><author><name sortKey="Langrish, C J" uniqKey="Langrish C">C.J. Langrish</name></author><author><name sortKey="Barrett, N A" uniqKey="Barrett N">N.A. Barrett</name></author><author><name sortKey="Sallusto, F" uniqKey="Sallusto F">F. Sallusto</name></author><author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name></author><author><name sortKey="Varani, L" uniqKey="Varani L">L. Varani</name></author><author><name sortKey="Zambon, M" uniqKey="Zambon M">M. Zambon</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S. Perlman</name></author><author><name sortKey="Lanzavecchia, A" uniqKey="Lanzavecchia A">A. Lanzavecchia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cui, J" uniqKey="Cui J">J. Cui</name></author><author><name sortKey="Eden, J S" uniqKey="Eden J">J.-S. Eden</name></author><author><name sortKey="Holmes, E C" uniqKey="Holmes E">E.C. Holmes</name></author><author><name sortKey="Wang, L F" uniqKey="Wang L">L.-F. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Wit, E" uniqKey="De Wit E">E. de Wit</name></author><author><name sortKey="Feldmann, F" uniqKey="Feldmann F">F. Feldmann</name></author><author><name sortKey="Horne, E" uniqKey="Horne E">E. Horne</name></author><author><name sortKey="Martellaro, C" uniqKey="Martellaro C">C. Martellaro</name></author><author><name sortKey="Haddock, E" uniqKey="Haddock E">E. Haddock</name></author><author><name sortKey="Bushmaker, T" uniqKey="Bushmaker T">T. Bushmaker</name></author><author><name sortKey="Rosenke, K" uniqKey="Rosenke K">K. Rosenke</name></author><author><name sortKey="Okumura, A" uniqKey="Okumura A">A. Okumura</name></author><author><name sortKey="Rosenke, R" uniqKey="Rosenke R">R. Rosenke</name></author><author><name sortKey="Saturday, G" uniqKey="Saturday G">G. Saturday</name></author><author><name sortKey="Scott, D" uniqKey="Scott D">D. Scott</name></author><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H. Feldmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Wit, E" uniqKey="De Wit E">E. de Wit</name></author><author><name sortKey="Munster, V J" uniqKey="Munster V">V.J. Munster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L. Du</name></author><author><name sortKey="Yang, Y" uniqKey="Yang Y">Y. Yang</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y. Zhou</name></author><author><name sortKey="Lu, L" uniqKey="Lu L">L. Lu</name></author><author><name sortKey="Li, F" uniqKey="Li F">F. Li</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S. Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Duraiyan, J" uniqKey="Duraiyan J">J. Duraiyan</name></author><author><name sortKey="Govindarajan, R" uniqKey="Govindarajan R">R. Govindarajan</name></author><author><name sortKey="Kaliyappan, K" uniqKey="Kaliyappan K">K. Kaliyappan</name></author><author><name sortKey="Palanisamy, M" uniqKey="Palanisamy M">M. Palanisamy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Falzarano, D" uniqKey="Falzarano D">D. Falzarano</name></author><author><name sortKey="Kamissoko, B" uniqKey="Kamissoko B">B. Kamissoko</name></author><author><name sortKey="De Wit, E" uniqKey="De Wit E">E. de Wit</name></author><author><name sortKey="Maiga, O" uniqKey="Maiga O">O. Maiga</name></author><author><name sortKey="Cronin, J" uniqKey="Cronin J">J. Cronin</name></author><author><name sortKey="Samake, K" uniqKey="Samake K">K. Samake</name></author><author><name sortKey="Traore, A" uniqKey="Traore A">A. Traore</name></author><author><name sortKey="Milne Price, S" uniqKey="Milne Price S">S. Milne-Price</name></author><author><name sortKey="Munster, V J" uniqKey="Munster V">V.J. Munster</name></author><author><name sortKey="Sogoba, N" uniqKey="Sogoba N">N. Sogoba</name></author><author><name sortKey="Niang, M" uniqKey="Niang M">M. Niang</name></author><author><name sortKey="Safronetz, D" uniqKey="Safronetz D">D. Safronetz</name></author><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H. Feldmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author><author><name sortKey="Al Dhahiry, S H" uniqKey="Al Dhahiry S">S.H. Al Dhahiry</name></author><author><name sortKey="Reusken, C B" uniqKey="Reusken C">C.B. Reusken</name></author><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="Galiano, M" uniqKey="Galiano M">M. Galiano</name></author><author><name sortKey="Myers, R" uniqKey="Myers R">R. Myers</name></author><author><name sortKey="Godeke, G J" uniqKey="Godeke G">G.J. Godeke</name></author><author><name sortKey="Jonges, M" uniqKey="Jonges M">M. Jonges</name></author><author><name sortKey="Farag, E" uniqKey="Farag E">E. Farag</name></author><author><name sortKey="Diab, A" uniqKey="Diab A">A. Diab</name></author><author><name sortKey="Ghobashy, H" uniqKey="Ghobashy H">H. Ghobashy</name></author><author><name sortKey="Alhajri, F" uniqKey="Alhajri F">F. Alhajri</name></author><author><name sortKey="Al Thani, M" uniqKey="Al Thani M">M. Al-Thani</name></author><author><name sortKey="Al Marri, S A" uniqKey="Al Marri S">S.A. Al-Marri</name></author><author><name sortKey="Al Romaihi, H E" uniqKey="Al Romaihi H">H.E. Al Romaihi</name></author><author><name sortKey="Al Khal, A" uniqKey="Al Khal A">A. Al Khal</name></author><author><name sortKey="Bermingham, A" uniqKey="Bermingham A">A. Bermingham</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author><author><name sortKey="Alhajri, M M" uniqKey="Alhajri M">M.M. AlHajri</name></author><author><name sortKey="Koopmans, M P" uniqKey="Koopmans M">M.P. Koopmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author><author><name sortKey="Van Den Brand, J M" uniqKey="Van Den Brand J">J.M. van den Brand</name></author><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="Volz, A" uniqKey="Volz A">A. Volz</name></author><author><name sortKey="Wohlsein, P" uniqKey="Wohlsein P">P. Wohlsein</name></author><author><name sortKey="Smits, S L" uniqKey="Smits S">S.L. Smits</name></author><author><name sortKey="Schipper, D" uniqKey="Schipper D">D. Schipper</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Okba, N" uniqKey="Okba N">N. Okba</name></author><author><name sortKey="Fux, R" uniqKey="Fux R">R. Fux</name></author><author><name sortKey="Bensaid, A" uniqKey="Bensaid A">A. Bensaid</name></author><author><name sortKey="Solanes Foz, D" uniqKey="Solanes Foz D">D. Solanes Foz</name></author><author><name sortKey="Kuiken, T" uniqKey="Kuiken T">T. Kuiken</name></author><author><name sortKey="Baumgartner, W" uniqKey="Baumgartner W">W. Baumgartner</name></author><author><name sortKey="Segales, J" uniqKey="Segales J">J. Segales</name></author><author><name sortKey="Sutter, G" uniqKey="Sutter G">G. Sutter</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haverkamp, A K" uniqKey="Haverkamp A">A.K. Haverkamp</name></author><author><name sortKey="Lehmbecker, A" uniqKey="Lehmbecker A">A. Lehmbecker</name></author><author><name sortKey="Spitzbarth, I" uniqKey="Spitzbarth I">I. Spitzbarth</name></author><author><name sortKey="Widagdo, W" uniqKey="Widagdo W">W. Widagdo</name></author><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author><author><name sortKey="Segales, J" uniqKey="Segales J">J. Segales</name></author><author><name sortKey="Vergara Alert, J" uniqKey="Vergara Alert J">J. Vergara-Alert</name></author><author><name sortKey="Bensaid, A" uniqKey="Bensaid A">A. Bensaid</name></author><author><name sortKey="Van Den Brand, J M A" uniqKey="Van Den Brand J">J.M.A. van den Brand</name></author><author><name sortKey="Osterhaus, A" uniqKey="Osterhaus A">A. Osterhaus</name></author><author><name sortKey="Baumgartner, W" uniqKey="Baumgartner W">W. Baumgartner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hemida, M G" uniqKey="Hemida M">M.G. Hemida</name></author><author><name sortKey="Elmoslemany, A" uniqKey="Elmoslemany A">A. Elmoslemany</name></author><author><name sortKey="Al Hizab, F" uniqKey="Al Hizab F">F. Al-Hizab</name></author><author><name sortKey="Alnaeem, A" uniqKey="Alnaeem A">A. Alnaeem</name></author><author><name sortKey="Almathen, F" uniqKey="Almathen F">F. Almathen</name></author><author><name sortKey="Faye, B" uniqKey="Faye B">B. Faye</name></author><author><name sortKey="Chu, D K W" uniqKey="Chu D">D.K.W. Chu</name></author><author><name sortKey="Perera, R A P M" uniqKey="Perera R">R.A.P.M. Perera</name></author><author><name sortKey="Peiris, M" uniqKey="Peiris M">M. Peiris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Houser, K V" uniqKey="Houser K">K.V. Houser</name></author><author><name sortKey="Gretebeck, L" uniqKey="Gretebeck L">L. Gretebeck</name></author><author><name sortKey="Ying, T" uniqKey="Ying T">T. Ying</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Vogel, L" uniqKey="Vogel L">L. Vogel</name></author><author><name sortKey="Lamirande, E W" uniqKey="Lamirande E">E.W. Lamirande</name></author><author><name sortKey="Bock, K W" uniqKey="Bock K">K.W. Bock</name></author><author><name sortKey="Moore, I N" uniqKey="Moore I">I.N. Moore</name></author><author><name sortKey="Dimitrov, D S" uniqKey="Dimitrov D">D.S. Dimitrov</name></author><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K. Subbarao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, L" uniqKey="Jiang L">L. Jiang</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N. Wang</name></author><author><name sortKey="Zuo, T" uniqKey="Zuo T">T. Zuo</name></author><author><name sortKey="Shi, X" uniqKey="Shi X">X. Shi</name></author><author><name sortKey="Poon, K M" uniqKey="Poon K">K.M. Poon</name></author><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y. Wu</name></author><author><name sortKey="Gao, F" uniqKey="Gao F">F. Gao</name></author><author><name sortKey="Li, D" uniqKey="Li D">D. Li</name></author><author><name sortKey="Wang, R" uniqKey="Wang R">R. Wang</name></author><author><name sortKey="Guo, J" uniqKey="Guo J">J. Guo</name></author><author><name sortKey="Fu, L" uniqKey="Fu L">L. Fu</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author><author><name sortKey="Zheng, B J" uniqKey="Zheng B">B.J. Zheng</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X. Wang</name></author><author><name sortKey="Zhang, L" uniqKey="Zhang L">L. Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Johnson, R F" uniqKey="Johnson R">R.F. Johnson</name></author><author><name sortKey="Bagci, U" uniqKey="Bagci U">U. Bagci</name></author><author><name sortKey="Keith, L" uniqKey="Keith L">L. Keith</name></author><author><name sortKey="Tang, X" uniqKey="Tang X">X. Tang</name></author><author><name sortKey="Mollura, D J" uniqKey="Mollura D">D.J. Mollura</name></author><author><name sortKey="Zeitlin, L" uniqKey="Zeitlin L">L. Zeitlin</name></author><author><name sortKey="Qin, J" uniqKey="Qin J">J. Qin</name></author><author><name sortKey="Huzella, L" uniqKey="Huzella L">L. Huzella</name></author><author><name sortKey="Bartos, C J" uniqKey="Bartos C">C.J. Bartos</name></author><author><name sortKey="Bohorova, N" uniqKey="Bohorova N">N. Bohorova</name></author><author><name sortKey="Bohorov, O" uniqKey="Bohorov O">O. Bohorov</name></author><author><name sortKey="Goodman, C" uniqKey="Goodman C">C. Goodman</name></author><author><name sortKey="Kim, D H" uniqKey="Kim D">D.H. Kim</name></author><author><name sortKey="Paulty, M H" uniqKey="Paulty M">M.H. Paulty</name></author><author><name sortKey="Velasco, J" uniqKey="Velasco J">J. Velasco</name></author><author><name sortKey="Whaley, K J" uniqKey="Whaley K">K.J. Whaley</name></author><author><name sortKey="Johnson, J C" uniqKey="Johnson J">J.C. Johnson</name></author><author><name sortKey="Pettitt, J" uniqKey="Pettitt J">J. Pettitt</name></author><author><name sortKey="Ork, B L" uniqKey="Ork B">B.L. Ork</name></author><author><name sortKey="Solomon, J" uniqKey="Solomon J">J. Solomon</name></author><author><name sortKey="Oberlander, N" uniqKey="Oberlander N">N. Oberlander</name></author><author><name sortKey="Zhu, Q" uniqKey="Zhu Q">Q. Zhu</name></author><author><name sortKey="Sun, J" uniqKey="Sun J">J. Sun</name></author><author><name sortKey="Holbrook, M R" uniqKey="Holbrook M">M.R. Holbrook</name></author><author><name sortKey="Olinger, G G" uniqKey="Olinger G">G.G. Olinger</name></author><author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name></author><author><name sortKey="Hensley, L E" uniqKey="Hensley L">L.E. Hensley</name></author><author><name sortKey="Jahrling, P B" uniqKey="Jahrling P">P.B. Jahrling</name></author><author><name sortKey="Marasco, W A" uniqKey="Marasco W">W.A. Marasco</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Hulswit, R J G" uniqKey="Hulswit R">R.J.G. Hulswit</name></author><author><name sortKey="Widjaja, I" uniqKey="Widjaja I">I. Widjaja</name></author><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="Mcbride, R" uniqKey="Mcbride R">R. McBride</name></author><author><name sortKey="Peng, W" uniqKey="Peng W">W. Peng</name></author><author><name sortKey="Widagdo, W" uniqKey="Widagdo W">W. Widagdo</name></author><author><name sortKey="Tortorici, M A" uniqKey="Tortorici M">M.A. Tortorici</name></author><author><name sortKey="Van Dieren, B" uniqKey="Van Dieren B">B. van Dieren</name></author><author><name sortKey="Lang, Y" uniqKey="Lang Y">Y. Lang</name></author><author><name sortKey="Van Lent, J W M" uniqKey="Van Lent J">J.W.M. van Lent</name></author><author><name sortKey="Paulson, J C" uniqKey="Paulson J">J.C. Paulson</name></author><author><name sortKey="De Haan, C A M" uniqKey="De Haan C">C.A.M. de Haan</name></author><author><name sortKey="De Groot, R J" uniqKey="De Groot R">R.J. de Groot</name></author><author><name sortKey="Van Kuppeveld, F J M" uniqKey="Van Kuppeveld F">F.J.M. van Kuppeveld</name></author><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.-J. Bosch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Wan, Y" uniqKey="Wan Y">Y. Wan</name></author><author><name sortKey="Liu, P" uniqKey="Liu P">P. Liu</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J. Zhao</name></author><author><name sortKey="Lu, G" uniqKey="Lu G">G. Lu</name></author><author><name sortKey="Qi, J" uniqKey="Qi J">J. Qi</name></author><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q. Wang</name></author><author><name sortKey="Lu, X" uniqKey="Lu X">X. Lu</name></author><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y. Wu</name></author><author><name sortKey="Liu, W" uniqKey="Liu W">W. Liu</name></author><author><name sortKey="Zhang, B" uniqKey="Zhang B">B. Zhang</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S. Perlman</name></author><author><name sortKey="Gao, G F" uniqKey="Gao G">G.F. Gao</name></author><author><name sortKey="Yan, J" uniqKey="Yan J">J. Yan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, G" uniqKey="Lu G">G. Lu</name></author><author><name sortKey="Hu, Y" uniqKey="Hu Y">Y. Hu</name></author><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q. Wang</name></author><author><name sortKey="Qi, J" uniqKey="Qi J">J. Qi</name></author><author><name sortKey="Gao, F" uniqKey="Gao F">F. Gao</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y. Zhang</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W. Zhang</name></author><author><name sortKey="Yuan, Y" uniqKey="Yuan Y">Y. Yuan</name></author><author><name sortKey="Bao, J" uniqKey="Bao J">J. Bao</name></author><author><name sortKey="Zhang, B" uniqKey="Zhang B">B. Zhang</name></author><author><name sortKey="Shi, Y" uniqKey="Shi Y">Y. Shi</name></author><author><name sortKey="Yan, J" uniqKey="Yan J">J. Yan</name></author><author><name sortKey="Gao, G F" uniqKey="Gao G">G.F. Gao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name></author><author><name sortKey="Mishra, N" uniqKey="Mishra N">N. Mishra</name></author><author><name sortKey="Olival, K J" uniqKey="Olival K">K.J. Olival</name></author><author><name sortKey="Fagbo, S F" uniqKey="Fagbo S">S.F. Fagbo</name></author><author><name sortKey="Kapoor, V" uniqKey="Kapoor V">V. Kapoor</name></author><author><name sortKey="Epstein, J H" uniqKey="Epstein J">J.H. Epstein</name></author><author><name sortKey="Alhakeem, R" uniqKey="Alhakeem R">R. AlHakeem</name></author><author><name sortKey="Durosinloun, A" uniqKey="Durosinloun A">A. Durosinloun</name></author><author><name sortKey="Al Asmari, M" uniqKey="Al Asmari M">M. Al Asmari</name></author><author><name sortKey="Islam, A" uniqKey="Islam A">A. Islam</name></author><author><name sortKey="Kapoor, A" uniqKey="Kapoor A">A. Kapoor</name></author><author><name sortKey="Briese, T" uniqKey="Briese T">T. Briese</name></author><author><name sortKey="Daszak, P" uniqKey="Daszak P">P. Daszak</name></author><author><name sortKey="Al Rabeeah, A A" uniqKey="Al Rabeeah A">A.A. Al Rabeeah</name></author><author><name sortKey="Lipkin, W I" uniqKey="Lipkin W">W.I. Lipkin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mou, H" uniqKey="Mou H">H. Mou</name></author><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="Van Kuppeveld, F J" uniqKey="Van Kuppeveld F">F.J. van Kuppeveld</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J. Rottier</name></author><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Munster, V J" uniqKey="Munster V">V.J. Munster</name></author><author><name sortKey="Adney, D R" uniqKey="Adney D">D.R. Adney</name></author><author><name sortKey="Van Doremalen, N" uniqKey="Van Doremalen N">N. van Doremalen</name></author><author><name sortKey="Brown, V R" uniqKey="Brown V">V.R. Brown</name></author><author><name sortKey="Miazgowicz, K L" uniqKey="Miazgowicz K">K.L. Miazgowicz</name></author><author><name sortKey="Milne Price, S" uniqKey="Milne Price S">S. Milne-Price</name></author><author><name sortKey="Bushmaker, T" uniqKey="Bushmaker T">T. Bushmaker</name></author><author><name sortKey="Rosenke, R" uniqKey="Rosenke R">R. Rosenke</name></author><author><name sortKey="Scott, D" uniqKey="Scott D">D. Scott</name></author><author><name sortKey="Hawkinson, A" uniqKey="Hawkinson A">A. Hawkinson</name></author><author><name sortKey="De Wit, E" uniqKey="De Wit E">E. de Wit</name></author><author><name sortKey="Schountz, T" uniqKey="Schountz T">T. Schountz</name></author><author><name sortKey="Bowen, R A" uniqKey="Bowen R">R.A. Bowen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pallesen, J" uniqKey="Pallesen J">J. Pallesen</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N. Wang</name></author><author><name sortKey="Corbett, K S" uniqKey="Corbett K">K.S. Corbett</name></author><author><name sortKey="Wrapp, D" uniqKey="Wrapp D">D. Wrapp</name></author><author><name sortKey="Kirchdoerfer, R N" uniqKey="Kirchdoerfer R">R.N. Kirchdoerfer</name></author><author><name sortKey="Turner, H L" uniqKey="Turner H">H.L. Turner</name></author><author><name sortKey="Cottrell, C A" uniqKey="Cottrell C">C.A. Cottrell</name></author><author><name sortKey="Becker, M M" uniqKey="Becker M">M.M. Becker</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L. Wang</name></author><author><name sortKey="Shi, W" uniqKey="Shi W">W. Shi</name></author><author><name sortKey="Kong, W P" uniqKey="Kong W">W.-P. Kong</name></author><author><name sortKey="Andres, E L" uniqKey="Andres E">E.L. Andres</name></author><author><name sortKey="Kettenbach, A N" uniqKey="Kettenbach A">A.N. Kettenbach</name></author><author><name sortKey="Denison, M R" uniqKey="Denison M">M.R. Denison</name></author><author><name sortKey="Chappell, J D" uniqKey="Chappell J">J.D. Chappell</name></author><author><name sortKey="Graham, B S" uniqKey="Graham B">B.S. Graham</name></author><author><name sortKey="Ward, A B" uniqKey="Ward A">A.B. Ward</name></author><author><name sortKey="Mclellan, J S" uniqKey="Mclellan J">J.S. McLellan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pascal, K E" uniqKey="Pascal K">K.E. Pascal</name></author><author><name sortKey="Coleman, C M" uniqKey="Coleman C">C.M. Coleman</name></author><author><name sortKey="Mujica, A O" uniqKey="Mujica A">A.O. Mujica</name></author><author><name sortKey="Kamat, V" uniqKey="Kamat V">V. Kamat</name></author><author><name sortKey="Badithe, A" uniqKey="Badithe A">A. Badithe</name></author><author><name sortKey="Fairhurst, J" uniqKey="Fairhurst J">J. Fairhurst</name></author><author><name sortKey="Hunt, C" uniqKey="Hunt C">C. Hunt</name></author><author><name sortKey="Strein, J" uniqKey="Strein J">J. Strein</name></author><author><name sortKey="Berrebi, A" uniqKey="Berrebi A">A. Berrebi</name></author><author><name sortKey="Sisk, J M" uniqKey="Sisk J">J.M. Sisk</name></author><author><name sortKey="Matthews, K L" uniqKey="Matthews K">K.L. Matthews</name></author><author><name sortKey="Babb, R" uniqKey="Babb R">R. Babb</name></author><author><name sortKey="Chen, G" uniqKey="Chen G">G. Chen</name></author><author><name sortKey="Lai, K M" uniqKey="Lai K">K.M. Lai</name></author><author><name sortKey="Huang, T T" uniqKey="Huang T">T.T. Huang</name></author><author><name sortKey="Olson, W" uniqKey="Olson W">W. Olson</name></author><author><name sortKey="Yancopoulos, G D" uniqKey="Yancopoulos G">G.D. Yancopoulos</name></author><author><name sortKey="Stahl, N" uniqKey="Stahl N">N. Stahl</name></author><author><name sortKey="Frieman, M B" uniqKey="Frieman M">M.B. Frieman</name></author><author><name sortKey="Kyratsous, C A" uniqKey="Kyratsous C">C.A. Kyratsous</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qiu, H" uniqKey="Qiu H">H. Qiu</name></author><author><name sortKey="Sun, S" uniqKey="Sun S">S. Sun</name></author><author><name sortKey="Xiao, H" uniqKey="Xiao H">H. Xiao</name></author><author><name sortKey="Feng, J" uniqKey="Feng J">J. Feng</name></author><author><name sortKey="Guo, Y" uniqKey="Guo Y">Y. Guo</name></author><author><name sortKey="Tai, W" uniqKey="Tai W">W. Tai</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Du, L" uniqKey="Du L">L. Du</name></author><author><name sortKey="Zhao, G" uniqKey="Zhao G">G. Zhao</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y. Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="Mou, H" uniqKey="Mou H">H. Mou</name></author><author><name sortKey="Smits, S L" uniqKey="Smits S">S.L. Smits</name></author><author><name sortKey="Dekkers, D H W" uniqKey="Dekkers D">D.H.W. Dekkers</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name></author><author><name sortKey="Dijkman, R" uniqKey="Dijkman R">R. Dijkman</name></author><author><name sortKey="Muth, D" uniqKey="Muth D">D. Muth</name></author><author><name sortKey="Demmers, J A A" uniqKey="Demmers J">J.A.A. Demmers</name></author><author><name sortKey="Zaki, A" uniqKey="Zaki A">A. Zaki</name></author><author><name sortKey="Fouchier, R A M" uniqKey="Fouchier R">R.A.M. Fouchier</name></author><author><name sortKey="Thiel, V" uniqKey="Thiel V">V. Thiel</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Rottier, P J M" uniqKey="Rottier P">P.J.M. Rottier</name></author><author><name sortKey="Osterhaus, A D M E" uniqKey="Osterhaus A">A.D.M.E. Osterhaus</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reusken, C B" uniqKey="Reusken C">C.B. Reusken</name></author><author><name sortKey="Ababneh, M" uniqKey="Ababneh M">M. Ababneh</name></author><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name></author><author><name sortKey="Eljarah, A" uniqKey="Eljarah A">A. Eljarah</name></author><author><name sortKey="Abutarbush, S" uniqKey="Abutarbush S">S. Abutarbush</name></author><author><name sortKey="Godeke, G J" uniqKey="Godeke G">G.J. Godeke</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Zutt, I" uniqKey="Zutt I">I. Zutt</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J. Rottier</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author><author><name sortKey="Koopmans, M P" uniqKey="Koopmans M">M.P. Koopmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reusken, C B" uniqKey="Reusken C">C.B. Reusken</name></author><author><name sortKey="Schilp, C" uniqKey="Schilp C">C. Schilp</name></author><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="De Bruin, E" uniqKey="De Bruin E">E. De Bruin</name></author><author><name sortKey="Kohl, R H" uniqKey="Kohl R">R.H. Kohl</name></author><author><name sortKey="Farag, E A" uniqKey="Farag E">E.A. Farag</name></author><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author><author><name sortKey="Al Romaihi, H" uniqKey="Al Romaihi H">H. Al-Romaihi</name></author><author><name sortKey="Le Grange, F" uniqKey="Le Grange F">F. Le Grange</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Koopmans, M P" uniqKey="Koopmans M">M.P. Koopmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saqib, M" uniqKey="Saqib M">M. Saqib</name></author><author><name sortKey="Sieberg, A" uniqKey="Sieberg A">A. Sieberg</name></author><author><name sortKey="Hussain, M H" uniqKey="Hussain M">M.H. Hussain</name></author><author><name sortKey="Mansoor, M K" uniqKey="Mansoor M">M.K. Mansoor</name></author><author><name sortKey="Zohaib, A" uniqKey="Zohaib A">A. Zohaib</name></author><author><name sortKey="Lattwein, E" uniqKey="Lattwein E">E. Lattwein</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shi, S R" uniqKey="Shi S">S.-R. Shi</name></author><author><name sortKey="Shi, Y" uniqKey="Shi Y">Y. Shi</name></author><author><name sortKey="Taylor, C R" uniqKey="Taylor C">C.R. Taylor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smedley, R C" uniqKey="Smedley R">R.C. Smedley</name></author><author><name sortKey="Patterson, J S" uniqKey="Patterson J">J.S. Patterson</name></author><author><name sortKey="Miller, R" uniqKey="Miller R">R. Miller</name></author><author><name sortKey="Massey, J P" uniqKey="Massey J">J.P. Massey</name></author><author><name sortKey="Wise, A G" uniqKey="Wise A">A.G. Wise</name></author><author><name sortKey="Maes, R K" uniqKey="Maes R">R.K. Maes</name></author><author><name sortKey="Wu, P" uniqKey="Wu P">P. Wu</name></author><author><name sortKey="Kaneene, J B" uniqKey="Kaneene J">J.B. Kaneene</name></author><author><name sortKey="Kiupel, M" uniqKey="Kiupel M">M. Kiupel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tang, X C" uniqKey="Tang X">X.C. Tang</name></author><author><name sortKey="Agnihothram, S S" uniqKey="Agnihothram S">S.S. Agnihothram</name></author><author><name sortKey="Jiao, Y" uniqKey="Jiao Y">Y. Jiao</name></author><author><name sortKey="Stanhope, J" uniqKey="Stanhope J">J. Stanhope</name></author><author><name sortKey="Graham, R L" uniqKey="Graham R">R.L. Graham</name></author><author><name sortKey="Peterson, E C" uniqKey="Peterson E">E.C. Peterson</name></author><author><name sortKey="Avnir, Y" uniqKey="Avnir Y">Y. Avnir</name></author><author><name sortKey="Tallarico, A S" uniqKey="Tallarico A">A.S. Tallarico</name></author><author><name sortKey="Sheehan, J" uniqKey="Sheehan J">J. Sheehan</name></author><author><name sortKey="Zhu, Q" uniqKey="Zhu Q">Q. Zhu</name></author><author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name></author><author><name sortKey="Marasco, W A" uniqKey="Marasco W">W.A. Marasco</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name></author><author><name sortKey="De Graaf, M" uniqKey="De Graaf M">M. de Graaf</name></author><author><name sortKey="Lauber, C" uniqKey="Lauber C">C. Lauber</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. Zaki</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author><author><name sortKey="Gorbalenya, A E" uniqKey="Gorbalenya A">A.E. Gorbalenya</name></author><author><name sortKey="Snijder, E J" uniqKey="Snijder E">E.J. Snijder</name></author><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vergara Alert, J" uniqKey="Vergara Alert J">J. Vergara-Alert</name></author><author><name sortKey="Van Den Brand, J M" uniqKey="Van Den Brand J">J.M. van den Brand</name></author><author><name sortKey="Widagdo, W" uniqKey="Widagdo W">W. Widagdo</name></author><author><name sortKey="Munoz, Mt" uniqKey="Munoz M">Mt. Munoz</name></author><author><name sortKey="Raj, S" uniqKey="Raj S">S. Raj</name></author><author><name sortKey="Schipper, D" uniqKey="Schipper D">D. Schipper</name></author><author><name sortKey="Solanes, D" uniqKey="Solanes D">D. Solanes</name></author><author><name sortKey="Cordon, I" uniqKey="Cordon I">I. Cordon</name></author><author><name sortKey="Bensaid, A" uniqKey="Bensaid A">A. Bensaid</name></author><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author><author><name sortKey="Segales, J" uniqKey="Segales J">J. Segales</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walls, A C" uniqKey="Walls A">A.C. Walls</name></author><author><name sortKey="Tortorici, M A" uniqKey="Tortorici M">M.A. Tortorici</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Frenz, B" uniqKey="Frenz B">B. Frenz</name></author><author><name sortKey="Rottier, P J M" uniqKey="Rottier P">P.J.M. Rottier</name></author><author><name sortKey="Dimaio, F" uniqKey="Dimaio F">F. DiMaio</name></author><author><name sortKey="Rey, F A" uniqKey="Rey F">F.A. Rey</name></author><author><name sortKey="Veesler, D" uniqKey="Veesler D">D. Veesler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, N" uniqKey="Wang N">N. Wang</name></author><author><name sortKey="Shi, X" uniqKey="Shi X">X. Shi</name></author><author><name sortKey="Jiang, L" uniqKey="Jiang L">L. Jiang</name></author><author><name sortKey="Zhang, S" uniqKey="Zhang S">S. Zhang</name></author><author><name sortKey="Wang, D" uniqKey="Wang D">D. Wang</name></author><author><name sortKey="Tong, P" uniqKey="Tong P">P. Tong</name></author><author><name sortKey="Guo, D" uniqKey="Guo D">D. Guo</name></author><author><name sortKey="Fu, L" uniqKey="Fu L">L. Fu</name></author><author><name sortKey="Cui, Y" uniqKey="Cui Y">Y. Cui</name></author><author><name sortKey="Liu, X" uniqKey="Liu X">X. Liu</name></author><author><name sortKey="Arledge, K C" uniqKey="Arledge K">K.C. Arledge</name></author><author><name sortKey="Chen, Y H" uniqKey="Chen Y">Y.H. Chen</name></author><author><name sortKey="Zhang, L" uniqKey="Zhang L">L. Zhang</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Widjaja, I" uniqKey="Widjaja I">I. Widjaja</name></author><author><name sortKey="Wang, C" uniqKey="Wang C">C. Wang</name></author><author><name sortKey="Van Haperen, R" uniqKey="Van Haperen R">R. van Haperen</name></author><author><name sortKey="Gutierrez Lvarez, J" uniqKey="Gutierrez Lvarez J">J. Gutiérrez-Álvarez</name></author><author><name sortKey="Van Dieren, B" uniqKey="Van Dieren B">B. van Dieren</name></author><author><name sortKey="Okba, N M A" uniqKey="Okba N">N.M.A. Okba</name></author><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Fernandez Delgado, R" uniqKey="Fernandez Delgado R">R. Fernandez-Delgado</name></author><author><name sortKey="Grosveld, F" uniqKey="Grosveld F">F. Grosveld</name></author><author><name sortKey="Van Kuppeveld, F J M" uniqKey="Van Kuppeveld F">F.J.M. van Kuppeveld</name></author><author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L. Enjuanes</name></author><author><name sortKey="Drabek, D" uniqKey="Drabek D">D. Drabek</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.-J. Bosch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zumla, A" uniqKey="Zumla A">A. Zumla</name></author><author><name sortKey="Hui, D S" uniqKey="Hui D">D.S. Hui</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S. Perlman</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="brief-report"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Vet Immunol Immunopathol</journal-id><journal-id journal-id-type="iso-abbrev">Vet. Immunol. Immunopathol</journal-id><journal-title-group><journal-title>Veterinary Immunology and Immunopathology</journal-title></journal-title-group><issn pub-type="ppub">0165-2427</issn><issn pub-type="epub">1873-2534</issn><publisher><publisher-name>Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">31526954</article-id><article-id pub-id-type="pmc">7112921</article-id><article-id pub-id-type="publisher-id">S0165-2427(19)30171-0</article-id><article-id pub-id-type="doi">10.1016/j.vetimm.2019.109939</article-id><article-id pub-id-type="publisher-id">109939</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein</article-title></title-group><contrib-group><contrib contrib-type="author" id="aut0005"><name><surname>Haverkamp</surname><given-names>Ann-Kathrin</given-names></name><xref rid="aff0005" ref-type="aff">a</xref><xref rid="fn0005" ref-type="fn">1</xref></contrib><contrib contrib-type="author" id="aut0010"><name><surname>Bosch</surname><given-names>Berend J.</given-names></name><xref rid="aff0010" ref-type="aff">b</xref><xref rid="fn0005" ref-type="fn">1</xref></contrib><contrib contrib-type="author" id="aut0015"><name><surname>Spitzbarth</surname><given-names>Ingo</given-names></name><xref rid="aff0005" ref-type="aff">a</xref><xref rid="aff0015" ref-type="aff">c</xref></contrib><contrib contrib-type="author" id="aut0020"><name><surname>Lehmbecker</surname><given-names>Annika</given-names></name><xref rid="aff0005" ref-type="aff">a</xref><xref rid="aff0015" ref-type="aff">c</xref></contrib><contrib contrib-type="author" id="aut0025"><name><surname>Te</surname><given-names>Nigeer</given-names></name><xref rid="aff0020" ref-type="aff">d</xref></contrib><contrib contrib-type="author" id="aut0030"><name><surname>Bensaid</surname><given-names>Albert</given-names></name><xref rid="aff0020" ref-type="aff">d</xref></contrib><contrib contrib-type="author" id="aut0035"><name><surname>Segalés</surname><given-names>Joaquim</given-names></name><xref rid="aff0025" ref-type="aff">e</xref><xref rid="aff0030" ref-type="aff">f</xref></contrib><contrib contrib-type="author" id="aut0040"><name><surname>Baumgärtner</surname><given-names>Wolfgang</given-names></name><email>Wolfgang.Baumgaertner@tiho-hannover.de</email><xref rid="aff0005" ref-type="aff">a</xref><xref rid="aff0015" ref-type="aff">c</xref><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib></contrib-group><aff id="aff0005"><label>a</label>Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany</aff><aff id="aff0010"><label>b</label>Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, the Netherlands</aff><aff id="aff0015"><label>c</label>Center for Systems Neuroscience, 30559 Hannover, Germany</aff><aff id="aff0020"><label>d</label>IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain</aff><aff id="aff0025"><label>e</label>Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Bellaterra, Barcelona, Spain</aff><aff id="aff0030"><label>f</label>UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author at: Department of Pathology, University of Veterinary Medicine Hannover Foundation, Bünteweg 17, 30559 Hannover, Germany. <email>Wolfgang.Baumgaertner@tiho-hannover.de</email></corresp><fn id="fn0005"><label>1</label><p id="npar0005">both authors contributed equally.</p></fn></author-notes><pub-date pub-type="pmc-release"><day>9</day><month>9</month><year>2019</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>12</month><year>2019</year></pub-date><pub-date pub-type="epub"><day>9</day><month>9</month><year>2019</year></pub-date><volume>218</volume><fpage>109939</fpage><lpage>109939</lpage><history><date date-type="received"><day>10</day><month>5</month><year>2019</year></date><date date-type="rev-recd"><day>2</day><month>9</month><year>2019</year></date><date date-type="accepted"><day>8</day><month>9</month><year>2019</year></date></history><permissions><copyright-statement>© 2019 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2019</copyright-year><copyright-holder>Elsevier B.V.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract id="abs0005"><p>Middle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective antibodies which could be used as prophylaxis in prospective human epidemics. In the current study, eight human monoclonal antibodies (mAbs) with neutralizing and non-neutralizing capabilities, directed against different epitopes of the MERS-coronavirus (MERS-CoV) spike (MERS-S) protein, were investigated with regard to their ability to immunohistochemically detect respective epitopes on formalin-fixed paraffin-embedded (FFPE) nasal tissue sections of MERS-CoV experimentally infected alpacas. The most intense immunoreaction was detected using a neutralizing antibody directed against the receptor binding domain S1B of the MERS-S protein, which produced an immunosignal in the cytoplasm of ciliated respiratory epithelium and along the apical membranous region. A similar staining was obtained by two other mAbs which recognize the sialic acid-binding domain and the ectodomain of the membrane fusion subunit S2, respectively. Five mAbs lacked immunoreactivity for MERS-CoV antigen on FFPE tissue, even though they belong, at least in part, to the same epitope group. In summary, three tested human mAbs demonstrated capacity for detection of MERS-CoV antigen on FFPE samples and may be implemented in double or triple immunohistochemical methods.</p></abstract><kwd-group id="kwd0005"><title>Keywords</title><kwd>Immunohistochemistry</kwd><kwd>Middle East respiratory syndrome coronavirus</kwd><kwd>Spike protein</kwd><kwd>Monoclonal human antibodies</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000740 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000740 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:7112921
|texte= Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:31526954" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |