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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Vaccines for the prevention against the threat of MERS-CoV</title><author><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name><affiliation><nlm:aff id="AFF0001"><institution>Lindsley F. Kimball Research Institute, New York Blood Center</institution>, New York, NY,<country>USA</country></nlm:aff></affiliation></author><author><name sortKey="Tai, Wanbo" sort="Tai, Wanbo" uniqKey="Tai W" first="Wanbo" last="Tai">Wanbo Tai</name><affiliation><nlm:aff id="AFF0001"><institution>Lindsley F. Kimball Research Institute, New York Blood Center</institution>, New York, NY,<country>USA</country></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"><institution>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology</institution>, Beijing,<country>China</country></nlm:aff></affiliation></author><author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name><affiliation><nlm:aff id="AFF0002"><institution>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology</institution>, Beijing,<country>China</country></nlm:aff></affiliation></author><author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><affiliation><nlm:aff id="AFF0001"><institution>Lindsley F. Kimball Research Institute, New York Blood Center</institution>, New York, NY,<country>USA</country></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"><institution>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University</institution>, Shanghai,<country>China</country></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26985862</idno><idno type="pmc">5097835</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5097835</idno><idno type="RBID">PMC:5097835</idno><idno type="doi">10.1586/14760584.2016.1167603</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">001326</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001326</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Vaccines for the prevention against the threat of MERS-CoV</title><author><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name><affiliation><nlm:aff id="AFF0001"><institution>Lindsley F. Kimball Research Institute, New York Blood Center</institution>, New York, NY,<country>USA</country></nlm:aff></affiliation></author><author><name sortKey="Tai, Wanbo" sort="Tai, Wanbo" uniqKey="Tai W" first="Wanbo" last="Tai">Wanbo Tai</name><affiliation><nlm:aff id="AFF0001"><institution>Lindsley F. Kimball Research Institute, New York Blood Center</institution>, New York, NY,<country>USA</country></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"><institution>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology</institution>, Beijing,<country>China</country></nlm:aff></affiliation></author><author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name><affiliation><nlm:aff id="AFF0002"><institution>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology</institution>, Beijing,<country>China</country></nlm:aff></affiliation></author><author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><affiliation><nlm:aff id="AFF0001"><institution>Lindsley F. Kimball Research Institute, New York Blood Center</institution>, New York, NY,<country>USA</country></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"><institution>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University</institution>, Shanghai,<country>China</country></nlm:aff></affiliation></author></analytic><series><title level="j">Expert Review of Vaccines</title><idno type="ISSN">1476-0584</idno><idno type="eISSN">1744-8395</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>ABSTRACT</title><p>First identified in 2012, Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) is listed as a new Category C Priority Pathogen. While the high mortality of MERS-CoV infection is further intensified by potential human-to-human transmissibility, no MERS vaccines are available for human use. This review explains immune responses resulting from MERS-CoV infection, describes MERS vaccine criteria, and presents available small animal models to evaluate the efficacy of MERS vaccines. Current advances in vaccine development are summarized, focusing on specific applications and limitations of each vaccine category. Taken together, this review provides valuable guidelines toward the development of an effective and safe MERS vaccine. This article is written for a Special Focus Issue of <italic>Expert Review of Vaccines</italic> on ‘Vaccines for Biodefence’.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Ki, M" uniqKey="Ki M">M. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Expert Rev Vaccines</journal-id><journal-id journal-id-type="iso-abbrev">Expert Rev Vaccines</journal-id><journal-id journal-id-type="publisher-id">IERV</journal-id><journal-id journal-id-type="publisher-id">ierv20</journal-id><journal-title-group><journal-title>Expert Review of Vaccines</journal-title></journal-title-group><issn pub-type="ppub">1476-0584</issn><issn pub-type="epub">1744-8395</issn><publisher><publisher-name>Taylor & Francis</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26985862</article-id><article-id pub-id-type="pmc">5097835</article-id><article-id pub-id-type="publisher-id">1167603</article-id><article-id pub-id-type="doi">10.1586/14760584.2016.1167603</article-id><article-categories><subj-group subj-group-type="heading"><subject>Review</subject></subj-group></article-categories><title-group><article-title>Vaccines for the prevention against the threat of MERS-CoV</article-title><alt-title alt-title-type="running-authors">L. DU ET AL.</alt-title><alt-title alt-title-type="running-title">EXPERT REVIEW OF VACCINES</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Du</surname><given-names>Lanying</given-names></name><xref ref-type="aff" rid="AFF0001"><sup>a</sup></xref><xref ref-type="corresp" rid="AN0001"></xref></contrib><contrib contrib-type="author"><name><surname>Tai</surname><given-names>Wanbo</given-names></name><xref ref-type="aff" rid="AFF0001"><sup>a</sup></xref><xref ref-type="aff" rid="AFF0002"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zhou</surname><given-names>Yusen</given-names></name><xref ref-type="aff" rid="AFF0002"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Jiang</surname><given-names>Shibo</given-names></name><xref ref-type="aff" rid="AFF0001"><sup>a</sup></xref><xref ref-type="aff" rid="AFF0003"><sup>c</sup></xref></contrib><aff id="AFF0001"><label>a</label><institution>Lindsley F. Kimball Research Institute, New York Blood Center</institution>, New York, NY,<country>USA</country></aff><aff id="AFF0002"><label>b</label><institution>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology</institution>, Beijing,<country>China</country></aff><aff id="AFF0003"><label>c</label><institution>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University</institution>, Shanghai,<country>China</country></aff></contrib-group><author-notes><corresp id="AN0001"><bold>CONTACT</bold> Lanying Du <email xlink:href="ldu@nybc.org">ldu@nybc.org</email>; Shibo Jiang <email xlink:href="sjiang@nybc.org">sjiang@nybc.org</email></corresp></author-notes><pub-date pub-type="collection"><year>2016</year></pub-date><pub-date pub-type="epub"><day>06</day><month>4</month><year>2016</year></pub-date><volume>15</volume><issue>9</issue><issue-title content-type="special-issue">Vaccines for Biodefense</issue-title><fpage seq="8">1123</fpage><lpage>1134</lpage><history><date date-type="received"><day>01</day><month>1</month><year>2016</year></date><date date-type="accepted"><day>15</day><month>3</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 Informa UK Limited, trading as Taylor & Francis Group</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Informa UK Limited, trading as Taylor & Francis Group</copyright-holder><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="IERV_15_1167603.pdf"></self-uri><abstract><title>ABSTRACT</title><p>First identified in 2012, Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) is listed as a new Category C Priority Pathogen. While the high mortality of MERS-CoV infection is further intensified by potential human-to-human transmissibility, no MERS vaccines are available for human use. This review explains immune responses resulting from MERS-CoV infection, describes MERS vaccine criteria, and presents available small animal models to evaluate the efficacy of MERS vaccines. Current advances in vaccine development are summarized, focusing on specific applications and limitations of each vaccine category. Taken together, this review provides valuable guidelines toward the development of an effective and safe MERS vaccine. This article is written for a Special Focus Issue of <italic>Expert Review of Vaccines</italic> on ‘Vaccines for Biodefence’.</p></abstract><kwd-group kwd-group-type="author"><title>KEYWORDS</title><kwd>Animal models</kwd><kwd>immune responses</kwd><kwd>MERS</kwd><kwd>MERS-CoV</kwd><kwd>neutralizing antibody</kwd><kwd>protection</kwd><kwd>spike protein</kwd><kwd>vaccines</kwd></kwd-group><funding-group><award-group><funding-source><named-content content-type="funder-name">National Institute of Allergy and Infectious Diseases</named-content><named-content content-type="funder-identifier">10.13039/100000060</named-content></funding-source><award-id>AI109094</award-id></award-group></funding-group><counts><fig-count count="4"></fig-count><table-count count="2"></table-count><ref-count count="134"></ref-count><page-count count="12"></page-count></counts></article-meta></front><body><sec sec-type="intro" id="S0001"><title>Introduction</title><p>Since first emerging in Saudi Arabia in June 2012, cases of Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) infection have been reported from 26 countries. Saudi Arabia has the largest number of MERS cases, followed by South Korea. As of 2 February 2016, 1638 MERS cases, including 587 deaths (case fatality rate: ~36%), have been reported to the WHO [<xref rid="CIT0001">1</xref>–<xref rid="CIT0003">3</xref>]. Cases of MERS kept increasing in Saudi Arabia and reached at 1297 as of 24 February 2016 [<xref rid="CIT0004">4</xref>].</p><p>Similar to other coronaviruses (CoVs), including severe acute respiratory syndrome coronavirus (SARS-CoV) [<xref rid="CIT0005">5</xref>] and porcine epidemic diarrhea virus [<xref rid="CIT0006">6</xref>], MERS-CoV is a zoonotic virus and originates from bats, suggesting that bats are the most likely natural reservoir of MERS-CoV [<xref rid="CIT0007">7</xref>–<xref rid="CIT0010">10</xref>]. However, unlike SARS-CoV, which utilizes small animals, such as palm civets and raccoon dogs, as its intermediate hosts [<xref rid="CIT0011">11</xref>,<xref rid="CIT0012">12</xref>], MERS-CoV depends on dromedary camels [<xref rid="CIT0013">13</xref>–<xref rid="CIT0017">17</xref>]. Nevertheless, human-to-human transmission of MERS-CoV does occur, and unprepared health-care facilities could become a major source for human infection and transmission (<xref rid="F0001">Figure 1</xref>), as shown by the incidence of MERS-CoV cases in South Korea in 2015 [<xref rid="CIT0001">1</xref>,<xref rid="CIT0018">18</xref>–<xref rid="CIT0021">21</xref>]. Several family clusters infected with MERS-CoV have also been revealed [<xref rid="CIT0022">22</xref>–<xref rid="CIT0025">25</xref>]. Recently, MERS-CoV has been added to the NIAID list as a Category C Priority Pathogen [<xref rid="CIT0026">26</xref>]. This places it in the same category as SARS-CoV and, as such, it has the potential to be used as a biological weapon. Therefore, steps toward prevention strategies need to be taken, particularly the development of effective and safe vaccines.<fig id="F0001" fig-type="figure" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1586/14760584.2016.1167603-F0001</object-id><label>Figure 1.</label><caption><p>Potential MERS-CoV transmission routes and MERS-CoV-infection hosts.</p><p>Bats are the most likely natural reservoir of MERS-CoV, and dromedary camels are potential intermediate hosts. Human-to-human transmission of MERS-CoV may easily occur through healthcare facilities or within family clusters.</p></caption><graphic xlink:href="IERV_A_1167603_F0001_OC"></graphic></fig></p><p>CoV genera are classified as α, β, γ, and δ (<xref rid="F0002">Figure 2</xref>) [<xref rid="CIT0002">2</xref>,<xref rid="CIT0027">27</xref>–<xref rid="CIT0031">31</xref>]. The δ CoV, a new genus in the Coronaviridae family, usually infects birds, including HKU16, HKU17, and HKU21, or mammals, such as porcine deltacoronavirus HKU15 and new strains recently identified in United States, which causes porcine diarrhea [<xref rid="CIT0028">28</xref>,<xref rid="CIT0031">31</xref>–<xref rid="CIT0034">34</xref>]. Several α and β CoVs can infect humans, but only MERS-CoV and SARS-CoV have led to regional or global human outbreaks [<xref rid="CIT0001">1</xref>,<xref rid="CIT0002">2</xref>,<xref rid="CIT0020">20</xref>,<xref rid="CIT0035">35</xref>–<xref rid="CIT0040">40</xref>]. Although MERS-CoV and SARS-CoV are β CoVs, it is interesting to note that MERS-CoV is phylogenetically related to bat-CoVs HKU4 and HKU5 (<xref rid="F0002">Figure 2</xref>) [<xref rid="CIT0002">2</xref>,<xref rid="CIT0041">41</xref>–<xref rid="CIT0043">43</xref>]. Unlike HKU5, HKU4 can bind to bat and human dipeptidyl peptidase 4 (DPP4), the receptor of MERS-CoV, to directly infect bat cells, or indirectly infect a number of human cells by exogenous stimulation [<xref rid="CIT0008">8</xref>,<xref rid="CIT0044">44</xref>,<xref rid="CIT0045">45</xref>]. Two mutations, S746R and N762A, in the surface spike (S) protein may be responsible for transmitting MERS-CoV from bats to humans (<xref rid="F0001">Figure 1</xref>), thus explaining the origins of MERS-CoV in bats [<xref rid="CIT0007">7</xref>,<xref rid="CIT0009">9</xref>,<xref rid="CIT0044">44</xref>].<fig id="F0002" fig-type="figure" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1586/14760584.2016.1167603-F0002</object-id><label>Figure 2.</label><caption><p>Classification of coronavirus genera.</p><p>The four coronavirus genera are α, β, γ, and δ coronaviruses. Each coronavirus genus contains different subclasses. Letters in blue indicate coronaviruses that have caused human infection.</p></caption><graphic xlink:href="IERV_A_1167603_F0002_OC"></graphic></fig></p><p>Similar to other CoVs, the MERS-CoV genome is a single positive-stranded RNA. It contains two large replicase open reading frames (ORFs), ORF1a and ORF1b, encoding two proteases, a papain-like protease and 3C-like protease, which are conserved in all other CoVs [<xref rid="CIT0002">2</xref>,<xref rid="CIT0043">43</xref>,<xref rid="CIT0046">46</xref>]. The downstream ORF2, ORF6, ORF7, and ORF8 of MERS-CoV genome are believed to encode S, envelope (E), membrane (M), and nucleocapsid (N) structural proteins, respectively, all having different functions (<xref rid="F0003">Figure 3A</xref>,<xref rid="F0003">B</xref>) [<xref rid="CIT0002">2</xref>,<xref rid="CIT0043">43</xref>]. MERS-CoV E protein, for example, promotes virulence [<xref rid="CIT0046">46</xref>,<xref rid="CIT0047">47</xref>]. The surface S protein is composed of S1 and S2 subunits, respectively, responsible for cellular receptor DPP4 binding via the receptor-binding domain (RBD), and fusion of virus and cell membranes, thereby mediating the entry of MERS-CoV into target cells (<xref rid="F0003">Figure 3C</xref>) [<xref rid="CIT0045">45</xref>,<xref rid="CIT0048">48</xref>–<xref rid="CIT0050">50</xref>]. The MERS-CoV RBD consists of a core structure, which is homologous to that of the SARS-CoV S protein RBD, and a receptor-binding motif, which is unique to MERS-CoV, thus determining viral pathogenesis and receptor recognition [<xref rid="CIT0051">51</xref>–<xref rid="CIT0054">54</xref>]. In addition to the aforementioned major structural proteins, the MERS-CoV genome also encodes several accessory proteins, including 3, 4a, 4b, and 5, which, however, might not be essential for virus replication [<xref rid="CIT0027">27</xref>,<xref rid="CIT0043">43</xref>,<xref rid="CIT0047">47</xref>]. Recent studies have revealed that whole-genome consensus sequences of MERS-CoV from dromedary camels and humans are identical, further confirming MERS-CoV transmission from dromedary camels to humans [<xref rid="CIT0015">15</xref>,<xref rid="CIT0055">55</xref>].<fig id="F0003" fig-type="figure" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1586/14760584.2016.1167603-F0003</object-id><label>Figure 3.</label><caption><p>MERS-CoV genome and schematic structure of viral proteins.</p><p><bold>(A)</bold> The MERS-CoV genome consists of 2 partially overlapping replicase open reading frames (OFR1a and 1b) and several downstream ORFs that encode viral functional structural proteins and other proteins with unknown function. (<bold>B</bold>) Schematic structure of major MERS-CoV structural proteins. (<bold>C</bold>) Schematic structure of MERS-CoV S protein. SP, signal peptide; RBD, receptor-binding domain; RBM, receptor-binding motif; FP, fusion peptide; HR1 and HR2, heptad repeat 1 and 2; TM, transmembrane domain; CP, cytoplasmic tail.</p></caption><graphic xlink:href="IERV_A_1167603_F0003_OC"></graphic></fig></p></sec><sec id="S0002"><title>MERS-CoV infection and resulting immune responses</title><p>MERS-CoV infection may trigger antigen-specific humoral immune responses and neutralizing antibodies in camels and humans [<xref rid="CIT0056">56</xref>–<xref rid="CIT0059">59</xref>]. MERS-CoV- or S-specific antibodies, including those with neutralizing activity, were identified in dromedary camels from MERS-affected regions, including Saudi Arabia, Jordan, Qatar and, the United Arab Emirates [<xref rid="CIT0013">13</xref>,<xref rid="CIT0055">55</xref>,<xref rid="CIT0056">56</xref>,<xref rid="CIT0060">60</xref>]. In addition, the seroprevalence of MERS-CoV-specific antibodies was shown to be significantly higher in individuals exposed to camels than that found among the general population [<xref rid="CIT0014">14</xref>]. Studies on 37 MERS-CoV-infected adult patients indicated that all survivals had serum IgG and neutralizing antibodies, and the levels of such antibodies were weakly but inversely correlated with viral loads in the lower respiratory tracts [<xref rid="CIT0061">61</xref>]. In South Korea, MERS-CoV-infected humans also demonstrated a clear kinetics of serologic responses, including robust antibody responses developed at the early stage of the disease onset, but delayed antibody responses with neutralizing activity associated with later, more severe stages of the disease [<xref rid="CIT0057">57</xref>]. The above studies suggest that humoral immune responses, including neutralizing antibodies, play an important role in preventing MERS-CoV infection.</p><p>In addition to B-cell-mediated antibody responses, cellular immune responses mediated by specific T cells may play a supplementary role. The absence of IFNα in a patient who died from MERS-CoV infection could have impaired the production of antiviral adaptive IL-12- and IFN-γ-mediated Th1 immune responses, suggesting that IFNα might be important in the induction of robust cellular immune responses during the initial stage of disease progression [<xref rid="CIT0062">62</xref>]. Moreover, MERS-CoV infection may drive IL-17 production in humans, as well as the expression of cytokines and chemokines, including IL-12, IFN-γ, IP-10, and RANTES, in dendritic cells [<xref rid="CIT0063">63</xref>], effectively modulating innate immune responses.</p></sec><sec id="S0003"><title>Vaccine-induced immune responses and criteria for evaluating MERS vaccines</title><p>MERS vaccines can also induce humoral and cellular immune responses. Specifically, a good MERS vaccine should be able to induce strong humoral immune responses, particularly neutralizing antibodies, in vaccinated animals and humans, completely protecting immunized subjects from MERS-CoV challenge. Depending on the immunization routes, MERS vaccination may activate B cells to produce systemic IgG and/or secretory IgA (sIgA) antibodies, both of which can bind to the virus and, respectively, mediate systemic and mucosal immune responses [<xref rid="CIT0064">64</xref>–<xref rid="CIT0066">66</xref>]. Serum IgA could also be induced upon vaccination, particularly through the mucosal or intranasal (i.n.) route [<xref rid="CIT0065">65</xref>]. Antibodies with neutralizing activity can then neutralize MERS-CoV infection by blocking virus–target cell binding via cellular receptor DPP4, thus inhibiting virus entry (<xref rid="F0004">Figure 4</xref>) [<xref rid="CIT0067">67</xref>,<xref rid="CIT0068">68</xref>]. It is likely that some B cells will become antigen-specific memory B cells capable of activation by further boost immunization or other stimulation factors to induce rapid recall antibody responses [<xref rid="CIT0069">69</xref>], but this outcome has not been extensively studied in MERS-CoV-directed vaccines.<fig id="F0004" fig-type="figure" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1586/14760584.2016.1167603-F0004</object-id><label>Figure 4.</label><caption><p>Schematic diagram of MERS vaccine-induced immune responses and neutralization.</p><p>Immunization of MERS vaccines may activate naïve B cells to differentiate into plasma cells and produce serum IgG, IgA, and/or secretory immunoglobulin A (sIgA) antibodies to bind MERS-CoV. Antibodies with neutralizing activity will block binding between MERS-CoV and its receptor dipeptidyl peptidase-4 (DPP4) at the cell surface, thus inhibiting virus entry into target cells. Naïve CD4+ and CD8<sup>+</sup> T cells can also be activated to produce cytokines and/or function as cytotoxic T lymphocytes (CTLs) to destroy MERS-CoV-infected target cells. Some memory B (Bm) and T (Tm) cells may be activated after further stimulation or boost vaccination, and play a role in humoral and cellular immune responses.</p></caption><graphic xlink:href="IERV_A_1167603_F0004_OC"></graphic></fig></p><p>Immunization of MERS vaccines could induce antigen-specific T-cell immune responses as well. As such, CD4<sup>+</sup> T cells can be activated to secrete Th1, including IL-2, IFN-γ, and TNFα, and/or Th2, such as IL-4, IL-5, and IL-10, cytokines, in turn promoting cytotoxic T cells, such as T lymphocytes or CD8<sup>+</sup> T cells, to kill target cells infected with MERS-CoV (<xref rid="F0004">Figure 4</xref>) [<xref rid="CIT0065">65</xref>,<xref rid="CIT0070">70</xref>–<xref rid="CIT0072">72</xref>]. However, in animal models, neutralizing antibody alone was able to protect against challenge from MERS-CoV [<xref rid="CIT0064">64</xref>,<xref rid="CIT0073">73</xref>]; therefore, T-cell-mediated cellular immune responses, if needed, may play a supplementary role in preventing MERS-CoV infection [<xref rid="CIT0074">74</xref>,<xref rid="CIT0075">75</xref>].</p><p>An ideal MERS vaccine candidate should have high immunogenicity and strong potency, as judged by the ability to induce potent immune responses and neutralizing antibodies, as well as complete protection against MERS-CoV infection, with the lowest dosage and least injection time through an appropriate route. In addition, MERS vaccines need to maintain good safety without inducing virus-enhancing antibody or harmful immune responses, or causing immunopathological effects [<xref rid="CIT0027">27</xref>,<xref rid="CIT0075">75</xref>].</p></sec><sec id="S0004"><title>Current animal models for evaluating the <italic>in vivo</italic> efficacy of MERS vaccines</title><p>MERS vaccines need to be evaluated in appropriate animal models before proceeding to human clinical trials. Substantial progress has been made in the development of MERS animal models, including non-human primates (NHPs), such as rhesus macaques and common marmosets [<xref rid="CIT0076">76</xref>–<xref rid="CIT0079">79</xref>], as well as small animal models, such as hDPP4-transduced and transgenic (Tg) mice [<xref rid="CIT0073">73</xref>,<xref rid="CIT0080">80</xref>–<xref rid="CIT0083">83</xref>]. <xref rid="T0001">Table 1</xref> summarizes the currently available animal models, their characteristics, and potential applications for evaluating the efficacy of MERS vaccines.
<table-wrap id="T0001" orientation="landscape" position="float"><object-id pub-id-type="doi">10.1586/14760584.2016.1167603-T0001</object-id><label>Table 1.</label><caption><p>Current animal models being developed to evaluate the efficacy of MERS vaccines<sup>a</sup>.</p></caption><pmc-comment>OASIS TABLE HERE</pmc-comment><table frame="hsides" rules="groups"><colgroup><col width="1*" align="left"></col><col width="1*" align="left"></col><col width="1*" align="left"></col><col width="1*" align="left"></col><col width="1*" align="left"></col></colgroup><thead><tr><th align="left">Animal models</th><th align="center">Symptoms</th><th align="center">Characteristics</th><th align="center">Vaccines tested</th><th align="center">Refs.</th></tr></thead><tbody><tr><td align="left">Rhesus macaques</td><td align="left">Animals developed lower respiratory tract infection; MERS-CoV caused mild-to-marked interstitial pneumonia, with virus replication in alveolar pneumocytes; presented clinical signs; produced neutralizing antibodies</td><td align="left">Lacked severe disease; MERS-CoV tropism limited to lower respiratory tract</td><td align="left">DNA, DNA/protein, subunit vaccines</td><td align="left">[<xref rid="CIT0070">70</xref>,<xref rid="CIT0077">77</xref>,<xref rid="CIT0078">78</xref>,<xref rid="CIT0084">84</xref>]</td></tr><tr><td align="left">Common marmosets</td><td align="left">Animals developed severe pneumonia; extensive lesions and high viral loads in lungs detected</td><td align="left">Severe, partially lethal, disease model</td><td align="left">Not identified</td><td align="left">[<xref rid="CIT0079">79</xref>]</td></tr><tr><td align="left">Camels</td><td align="left">Animals developed upper respiratory tract infection with virus replication and clinical signs</td><td align="left">MERS-CoV tropism extended to upper respiratory tract</td><td align="left">DNA, viral vector vaccines</td><td align="left">[<xref rid="CIT0016">16</xref>,<xref rid="CIT0064">64</xref>,<xref rid="CIT0070">70</xref>]</td></tr><tr><td align="left">Rabbits</td><td align="left">Infectious virus detected in lungs and upper respiratory tract</td><td align="left">No significant histopathological changes or clinical signs of disease</td><td align="left">Not identified</td><td align="left">[<xref rid="CIT0085">85</xref>]</td></tr><tr><td align="left">Ad5-hDPP4 mice</td><td align="left">Animals developed pneumonia; showed clinical disease and histopathological changes in lungs</td><td align="left">Transient expression; no mortality</td><td align="left">Viral vector, subunit vaccines</td><td align="left">[<xref rid="CIT0072">72</xref>,<xref rid="CIT0083">83</xref>]</td></tr><tr><td align="left">Humanized (HuDPP4) mice</td><td align="left">Expressed HuDPP4 in lungs; virus replication and pathology detected in lungs</td><td align="left">No clinical signs of disease or mortality</td><td align="left">Not identified</td><td align="left">[<xref rid="CIT0086">86</xref>]</td></tr><tr><td align="left">hDPP4-Tg mice</td><td align="left">Animals developed progressive pneumonia; viral replication detected in lung and brain; produced neutralizing antibodies</td><td align="left">Lethal disease model</td><td align="left">Subunit vaccines; VRP vaccines</td><td align="left">[<xref rid="CIT0073">73</xref>,<xref rid="CIT0080">80</xref>–<xref rid="CIT0082">82</xref>]</td></tr></tbody></table><table-wrap-foot><fn><p><sup>a</sup>Ad5-hDPP4: Adenovirus 5-human DPP4-transduced mice; hDPP4: human dipeptidyl peptidase-4; hDPP4-Tg mice: human DPP4-transgenic mice; MERS: Middle East respiratory syndrome; MERS-CoV: Middle East respiratory syndrome coronavirus; VRP: virus replicon particle.</p></fn></table-wrap-foot></table-wrap></p><p>NHP models have been initially established as an effective vehicle for MERS-CoV infection and vaccine evaluation. Rhesus macaques infected with MERS-CoV developed lower respiratory tract symptoms with mild-to-moderate interstitial pneumonia, and virus replication mainly occurred in alveolar pneumocytes. Also, clinical signs of disease and neutralizing antibodies were produced in these animals upon virus infection [<xref rid="CIT0077">77</xref>,<xref rid="CIT0078">78</xref>]. In contrast, marmosets, as a new MERS-CoV infection model, developed a much more severe disease with progressive severe pneumonia, leading to significant viral replication in the lungs and partial lethality [<xref rid="CIT0079">79</xref>]. However, other reports demonstrated a mild-to-moderate nonlethal respiratory disease in common marmosets with limited additional clinical signs upon inoculation with MERS-CoV [<xref rid="CIT0087">87</xref>]. Except for NHPs, camels infected with MERS-CoV may present upper respiratory tract symptoms with virus replication and shedding in the upper respiratory tract of inoculated dromedary camels [<xref rid="CIT0016">16</xref>].</p><p>Unlike SARS-CoV, which easily infects commonly used laboratory animals, including Syrian hamsters, ferrets, and mice [<xref rid="CIT0088">88</xref>–<xref rid="CIT0091">91</xref>], MERS-CoV does not normally infect these animal species because of the differences in binding viral receptor [<xref rid="CIT0092">92</xref>–<xref rid="CIT0095">95</xref>]. Recently, a number of small animal models were developed for MERS-CoV [<xref rid="CIT0073">73</xref>,<xref rid="CIT0080">80</xref>–<xref rid="CIT0083">83</xref>]. For example, after prior transduction with adenovirus 5 expressing human DPP4 (Ad5-hDPP4), mice became sensitive to MERS-CoV infection and developed pneumonia accompanied by clinical disease and histopathological changes in the lungs [<xref rid="CIT0083">83</xref>]. In addition, a humanized (HuDPP4) mouse model was established, in which mouse DPP4 was replaced by human DPP4 [<xref rid="CIT0086">86</xref>]. In particular, a human DPP4 transgenic (hDPP4-Tg) mouse model globally expresses the hDPP4 receptor, and it is fully permissive to MERS-CoV infection [<xref rid="CIT0073">73</xref>,<xref rid="CIT0081">81</xref>]. Infected animals developed progressive pneumonia and demonstrated significant weight loss and death upon virus infection. Virus replication was detected in lung and brain [<xref rid="CIT0080">80</xref>–<xref rid="CIT0082">82</xref>]. Thus, such small animal models provide an economical, readily available method of testing the efficacy of MERS-CoV candidate vaccines.</p></sec><sec id="S0005"><title>Current advances in MERS vaccine development</title><p>No vaccines against MERS-CoV are currently available for human use. Nevertheless, progress has been made since the emergence of MERS-CoV in 2012, and a number of MERS vaccines have been developed and tested in preclinical stages [<xref rid="CIT0064">64</xref>,<xref rid="CIT0066">66</xref>,<xref rid="CIT0070">70</xref>,<xref rid="CIT0072">72</xref>,<xref rid="CIT0084">84</xref>,<xref rid="CIT0096">96</xref>,<xref rid="CIT0097">97</xref>], two of which are scheduled for human clinical trials [<xref rid="CIT0098">98</xref>,<xref rid="CIT0099">99</xref>]. These vaccines are based on recombinant virus; viral vectors, including modified vaccinia virus Ankara (MVA), adenovirus (Ad), and measles virus (MV); nanoparticles; DNA and DNA/protein; as well as subunit vaccines. <xref rid="T0002">Table 2</xref> summarizes current MERS vaccines under development.
<table-wrap id="T0002" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1586/14760584.2016.1167603-T0002</object-id><label>Table 2.</label><caption><p>Summary of current vaccines being developed to prevent MERS-CoV infection<sup>a</sup>.</p></caption><pmc-comment>OASIS TABLE HERE</pmc-comment><table frame="hsides" rules="groups"><colgroup><col width="1*" align="left"></col><col width="1*" align="left"></col><col width="1*" align="left"></col><col width="1*" align="left"></col><col width="1*" align="left"></col><col width="1*" align="left"></col></colgroup><thead><tr><th align="left">Vaccine categories</th><th align="center">Immunogenicity and protection</th><th align="center">Immunization routes</th><th align="center">Adjuvant needed</th><th align="center">Potential limitations</th><th align="center">Refs.</th></tr></thead><tbody><tr><td align="left">Recombinant MERS-CoV vaccines</td><td align="left">Not indicated</td><td align="left">N/A</td><td align="left">N/A</td><td align="left">Possibility of recovering virulence</td><td align="left">[<xref rid="CIT0046">46</xref>,<xref rid="CIT0047">47</xref>]</td></tr><tr><td align="left">Viral-vector-based vaccines</td><td align="left">Induced antigen-specific humoral (IgG) and/or T-cell immune responses, and neutralizing antibody in mice; protected hDPP4-transduced mice against MERS-CoV challenge; reduced MERS-CoV excretion after virus infection in dromedary camels</td><td align="left">i.g., i.m., s.c., or i.n.</td><td align="left">No</td><td align="left">Preexisting immunity; antivector responses; potential harmful immune responses by non-neutralizing epitopes of full-length S</td><td align="left">[<xref rid="CIT0064">64</xref>,<xref rid="CIT0072">72</xref>,<xref rid="CIT0097">97</xref>,<xref rid="CIT0100">100</xref>–<xref rid="CIT0102">102</xref>]</td></tr><tr><td align="left">Nanoparticles</td><td align="left">Induced MERS-CoV neutralizing antibody in mice in the presence of adjuvants, particularly Matrix M1</td><td align="left">i.m.</td><td align="left">Yes: Alum, Matrix M1</td><td align="left">Potential harmful immune responses by non-neutralizing epitopes of full-length S</td><td align="left">[<xref rid="CIT0096">96</xref>]</td></tr><tr><td align="left">DNA vaccines</td><td align="left">Induced antigen-specific neutralizing antibody and cellular immunity in mice, NHPs and camels; protected NHPs from MERS-CoV challenge</td><td align="left">i.m./AP system</td><td align="left">No</td><td align="left">Potential side effects; harmful immune responses by non-neutralizing epitopes of full-length S</td><td align="left">[<xref rid="CIT0070">70</xref>]</td></tr><tr><td align="left">DNA prime/protein-boost vaccines</td><td align="left">Induced robust serum-neutralizing antibody in mice and NHPs; protected NHPs from MERS-CoV challenge</td><td align="left">i.m./AP system</td><td align="left">Yes: Ribi, Alum, AlPO<sub>4</sub></td><td align="left">Potential harmful immune responses by non-neutralizing epitopes of full-length S</td><td align="left">[<xref rid="CIT0084">84</xref>]</td></tr><tr><td align="left">Subunit vaccines</td><td align="left">Induced strong humoral and mucosal immune responses and potent neutralizing antibody in mice and/or rabbits; elicited T-cell responses in mice; protected hDPP4-mice and NHPs from MERS-CoV challenge</td><td align="left">i.m., s.c., or i.n.</td><td align="left">Yes: Alum, MF59, Montanide, Poly(I:C)</td><td align="left">Need to maintain suitable protein conformation; require appropriate adjuvant, route, or dose</td><td align="left">[<xref rid="CIT0065">65</xref>,<xref rid="CIT0066">66</xref>,<xref rid="CIT0071">71</xref>,<xref rid="CIT0073">73</xref>,<xref rid="CIT0074">74</xref>,<xref rid="CIT0103">103</xref>,<xref rid="CIT0104">104</xref>]</td></tr></tbody></table><table-wrap-foot><fn><p><sup>a</sup>Alum: aluminum hydroxide; AlPO<sub>4</sub>: aluminum phosphate; AP system: AgilePulse® <italic>i</italic><italic>n vivo</italic> electroporation; hDPP4: human dipeptidyl peptidase-4; i.g.: intragastric; i.m.: intramuscular; i.n.: intranasal; s.c.: subcutaneous; MERS-CoV: Middle East respiratory syndrome coronavirus; S: spike.</p></fn></table-wrap-foot></table-wrap></p><sec id="S0005-S2001"><title>Recombinant MERS-CoV as vaccines</title><p>Unlike SARS vaccines that are usually developed based on attenuated or inactivated SARS-CoV, thus having a potential to recover virulence [<xref rid="CIT0011">11</xref>,<xref rid="CIT0105">105</xref>–<xref rid="CIT0108">108</xref>], MERS-CoV vaccines could be constructed based on recombinant viruses using reverse genetics. Accordingly, a recombinant MERS-CoV with expected marker mutations was generated using a panel of contiguous cDNAs spanning the entire viral genome and replicated to high titers with broad tissue tropism. Also, an engineered mutant MERS-CoV lacking the structural E protein was rescued and propagated in cells expressing the viral E protein in <italic>trans</italic> [<xref rid="CIT0046">46</xref>,<xref rid="CIT0047">47</xref>]. Using reverse genetics, it is possible to develop a replication-competent, propagation-defective MERS-CoV candidate vaccine, providing a platform for the design of live attenuated MERS-CoV vaccines. Since such recombinant MERS viruses still contain major virus components, their safety needs to be tested extensively, and their immunogenicity requires further evaluation in appropriate animal models.</p></sec><sec id="S0005-S2002"><title>Viral-vector-based MERS vaccines</title><p>Similar to viral vector-based SARS vaccines [<xref rid="CIT0109">109</xref>–<xref rid="CIT0111">111</xref>], MERS vaccines can also be constructed using viral vectors that express major MERS-CoV proteins, normally the S protein. Several such MERS vaccine candidates have been developed and/or tested for efficacy in hDPP4-expressing mouse models or camels [<xref rid="CIT0064">64</xref>,<xref rid="CIT0097">97</xref>,<xref rid="CIT0100">100</xref>,<xref rid="CIT0101">101</xref>].</p><p>Ad5 or Ad41 vector expressing full-length S or S1 protein of MERS-CoV-induced S-specific antibody responses and/or T-cell responses in a mouse model via the intramuscular (i.m.) or intragastric route, effectively neutralizing MERS-CoV infection <italic>in vitro</italic> [<xref rid="CIT0097">97</xref>,<xref rid="CIT0102">102</xref>]. Also, i.m. or subcutaneous (s.c.) vaccination of mice with a MVA-based full-length S vaccine elicited MERS-CoV-specific CD8<sup>+</sup> T-cell response and neutralizing antibodies, protecting hDPP4-transduced mice against MERS-CoV challenge [<xref rid="CIT0100">100</xref>,<xref rid="CIT0101">101</xref>]. Intranasally or intramuscularly administered MVA-S vaccine induced mucosal immunity, particularly the neutralizing antibodies, in dromedary camels, resulting in significant reduction of excreted infectious virus and viral RNA transcripts after MERS-CoV challenge [<xref rid="CIT0064">64</xref>]. Similarly, a recombinant MV-based MERS vaccine expressing full-length, or truncated, S protein of MERS-CoV induced robust MERS-CoV neutralizing antibodies and T-cell responses, protecting mice transduced with hDPP4 from MERS-CoV challenge [<xref rid="CIT0072">72</xref>].</p><p>Although able to elicit strong immune responses and/or protection, viral-vector-based vaccines might have some unwanted limitations in terms of safety and potency. For example, preexisting immunity to Ad in the general human population may cause some adverse effects by the induction of vaccine antigen-specific immune responses, thus reducing the overall efficacy of this vaccine type [<xref rid="CIT0112">112</xref>–<xref rid="CIT0114">114</xref>]. In addition, production of neutralizing antibodies against viral vectors themselves has been demonstrated in MV-S- and MVA-S-based MERS vaccines [<xref rid="CIT0064">64</xref>,<xref rid="CIT0072">72</xref>]. Furthermore, full-length S protein of SARS-CoV encoded by the vectors can also induce non-neutralizing antibodies that may mediate enhancement of virus infection or cause harmful immune responses, such as inflammation and enhanced hepatitis [<xref rid="CIT0115">115</xref>–<xref rid="CIT0117">117</xref>], special attention should be drawn when developing MERS-CoV full-length S protein-based viral vectored vaccines.</p></sec><sec id="S0005-S2003"><title>Nanoparticle-based MERS vaccines</title><p>Nanoparticles can be used as a delivery vehicle to develop MERS vaccines. Nanoparticles containing MERS-CoV full-length S protein can be prepared and purified from pellets of infected baculovirus insect cells. In the absence of adjuvants, these nanoparticles induced a lower level of MERS-CoV neutralizing antibodies in mice, while in the presence of adjuvants, such as aluminum hydroxide (Alum) or Matrix M1, such neutralizing antibodies were significantly increased and maintained. Also, Matrix M1 significantly promoted the production of neutralizing antibodies as compared with Alum [<xref rid="CIT0096">96</xref>]. Thus, adjuvants are required for MERS nanoparticle vaccines, and different adjuvants function differently in promoting the immunogenicity of these vaccines. Thus far, efficacy and protection have not been evaluated for this vaccine type in MERS-CoV challenge models.</p></sec><sec id="S0005-S2004"><title>DNA-based MERS vaccines</title><p>Like the full-length S gene of SARS-CoV, DNA encoding full-length S protein of MERS-CoV can also be utilized to develop MERS vaccines [<xref rid="CIT0070">70</xref>,<xref rid="CIT0118">118</xref>]. Indeed, i.m./electroporation of mice and rhesus macaques with a synthetic DNA encoding full-length S protein of MERS-CoV elicited potent virus-neutralizing antibodies and cellular immune responses, as represented by the secretion of INF-γ, TNF-α, and/or IL-2 cytokines in CD4<sup>+</sup> and/or CD8<sup>+</sup> T cells, as well as the production of neutralizing antibodies in immunized camels. In addition, immunized NHPs were protected against MERS-CoV challenge without demonstrating clinical or radiographic signs of pneumonia [<xref rid="CIT0070">70</xref>]. Since such DNA vaccines encode MERS-CoV full-length S protein, the potential induction of virus-enhancing antibody and harmful immune responses is possible.</p></sec><sec id="S0005-S2005"><title>DNA prime/protein boosted MERS vaccines</title><p>In addition to a DNA-alone vaccination strategy, DNA priming followed by protein boosting could be used to develop MERS vaccines and, as a result, expand the immunogenicity and efficacy generated by DNA. In this combinational vaccination strategy, DNA was constructed to encode full-length S protein of MERS-CoV, while protein was expressed as the viral S1 subunit [<xref rid="CIT0084">84</xref>]. Results demonstrated that i.m./electroporation priming of full-length S DNA and i.m. boosting of S1 protein of MERS-CoV with Ribi or Alum (aluminum phosphate, AlPO<sub>4</sub>) adjuvant in mice and rhesus macaques, respectively, induced robust neutralizing antibodies against MERS-CoV infection, conferring protection of NHPs against MERS-CoV-induced radiographic pneumonia. However, because of the containment of full-length S DNA in the vaccination regimen, the potential of vaccine-caused immunopathology needs to be investigated.</p></sec><sec id="S0005-S2006"><title>Subunit MERS vaccines</title><p>Protein-based subunit vaccines against MERS-CoV have been developed [<xref rid="CIT0066">66</xref>,<xref rid="CIT0067">67</xref>,<xref rid="CIT0071">71</xref>,<xref rid="CIT0103">103</xref>]. While some subunit vaccines are designed on the basis of the full-length S1 protein [<xref rid="CIT0084">84</xref>], the majority of them are based on viral RBD [<xref rid="CIT0066">66</xref>,<xref rid="CIT0067">67</xref>,<xref rid="CIT0071">71</xref>,<xref rid="CIT0103">103</xref>,<xref rid="CIT0119">119</xref>]. These RBD-based vaccines are evaluated for immunogenicity and protective immunity in a number of MERS-CoV animal models, including hDPP4-transduced and hDPP4-Tg mice, as well as NHPs [<xref rid="CIT0071">71</xref>,<xref rid="CIT0073">73</xref>,<xref rid="CIT0074">74</xref>,<xref rid="CIT0103">103</xref>,<xref rid="CIT0119">119</xref>,<xref rid="CIT0120">120</xref>]. The antigenicity and functionality of these RBD proteins have also been extensively investigated.</p><p>In general, subunit vaccines might not induce immune responses as strong as those induced by other vaccine types mentioned above. However, the immunogenicity of subunit vaccines could be significantly promoted in the presence of an ideal adjuvant via an appropriate route [<xref rid="CIT0065">65</xref>,<xref rid="CIT0074">74</xref>]. In addition, it is also essential to maintain a suitable conformation of the protein antigen in the vaccine, such as the MERS-CoV RBD proteins [<xref rid="CIT0066">66</xref>,<xref rid="CIT0067">67</xref>]. For example, both s.c. and i.n. immunization of MERS-CoV RBD protein adjuvanted with Montanide ISA51 or Poly(I:C) induced long-term, high titers of S-specific systemic IgG, IgA, and mucosal sIgA antibodies, potently neutralizing MERS-CoV infection [<xref rid="CIT0065">65</xref>]. After comparing several different RBD fragments of MERS-CoV S protein, a fragment containing residues 377–588 of RBD elicited the highest neutralizing antibody in mice and rabbits and was therefore identified as a critical neutralizing domain [<xref rid="CIT0066">66</xref>,<xref rid="CIT0068">68</xref>]. Moreover, since MF59 adjuvant improved the ability of RBD protein to elicit the highest titer of neutralizing antibodies of all adjuvants tested, it is considered an ideal adjuvant to use with RBD subunit vaccines [<xref rid="CIT0074">74</xref>]. Even low doses of the RBD antigen plus MF59 adjuvant elicited sufficient neutralizing antibodies against MERS-CoV infection [<xref rid="CIT0104">104</xref>]. In the presence of MF59 adjuvant, this RBD protein protected Ad5-hDPP4-transduced and hDPP4-Tg mice from MERS-CoV challenge [<xref rid="CIT0073">73</xref>,<xref rid="CIT0074">74</xref>]. Clearly, the identified critical neutralizing domain of MERS-CoV RBD protein maintained good conformational structure, strong antigenicity to bind specifically to MERS-CoV RBD-specific sera and neutralizing monoclonal antibodies, as well as intact functionality to interact with soluble and cell-associated hDPP4 receptors [<xref rid="CIT0066">66</xref>,<xref rid="CIT0068">68</xref>,<xref rid="CIT0121">121</xref>].</p><p>In terms of safety consideration, subunit vaccines should be accounted as the safest vaccine type. They do not contain viral genetic materials, but only include essential antigens for eliciting protective immune responses, thus excluding the possibility of recovering virulence or inducing adverse reactions [<xref rid="CIT0122">122</xref>–<xref rid="CIT0126">126</xref>]. Different from the vaccines based on the full-length S or S1 protein, RBD-based MERS subunit vaccines contain the major neutralizing epitopes and lack non-neutralizing immunodominant domains, thus having minimum risk to induce non-neutralizing antibodies with potential to cause harmful immune responses or enhancement of virus infection [<xref rid="CIT0027">27</xref>,<xref rid="CIT0066">66</xref>,<xref rid="CIT0075">75</xref>].</p></sec></sec><sec id="S0006"><title>Summary and conclusions</title><p>MERS-CoV, a newly emerging infectious CoV and a new Category C Priority Pathogen, has caused high mortality in humans, thus posing continual threats to public health and global safety. Since the emergence of MERS-CoV in 2012, tremendous progress has been made in the development of MERS vaccines and the evaluation of their efficacy in suitable animal models. Presently, no MERS vaccines are available for human use. This review explains immune responses resulting from MERS-CoV infection, describes MERS vaccine criteria, and presents available small animal models to evaluate the efficacy of MERS vaccines. Current advances in vaccine development are summarized, focusing on specific applications and limitations of each vaccine category. These MERS vaccines were categorized as recombinant virus, viral vectors, nanoparticles, DNAs, DNAs/proteins, and subunit vaccines, denoting specific applications and limitations of each category. Taken together, this review provides valuable guidelines toward the development of an effective and safe MERS vaccine.</p></sec><sec id="S0007"><title>Expert commentary</title><p>Several MERS candidate vaccines in development have demonstrated the ability to induce immune responses and/or neutralizing antibodies that protect against MERS-CoV infection. Based on the limitations of some of these vaccine candidates, as discussed above, it might be fruitful to establish standards against which to measure the specific role of humoral and cellular immune responses relative to protection against MERS-CoV infection, and further evaluate the efficacy and correlation between immunogenicity and protection.</p><p>In addition to efficacy, safety is an important issue for any MERS vaccine. Experience garnered from SARS vaccine studies has demonstrated that vaccines based on the full-length S protein of SARS-CoV may induce non-neutralizing antibodies with enhancing effect on virus infection or harmful immune responses, or cause immunopathological effect, such as inflammation and increased severity [<xref rid="CIT0115">115</xref>,<xref rid="CIT0116">116</xref>]. Thus, when developing MERS vaccines based on the full-length S protein, precautions should be taken against the induction of harmful immune responses and/or virus-enhancing antibodies potentially resulting from its non-neutralizing epitopes in the immunodominant domains. Concomitantly, the immunopathological effects of these MERS vaccines should be investigated. Other safety tests, such as toxicity experiments, are also recommended before moving a MERS vaccine candidate to human clinical trials or patient use.</p><p>One may argue that no virus-enhancing antibody induced by full-length S protein of MERS-CoV has been reported so far. Indeed, there had been no report on antibody-mediated enhancement of SARS-CoV infection for 8 years since the virus was first identified in Guangdong Province, China in 2003. However, Jaume et al. [<xref rid="CIT0117">117</xref>] reported in 2012 that a SARS vaccine based on the full-length S protein could induce in mice virus-neutralizing antibodies tested in Vero E6 cell culture, and virus-enhancing antibodies, via an FcγR-dependent manner, detected in cultures of THP-1, Raji, and Daudi cells that express FcγR. This finding suggests that virus-enhancing antibodies induced by the full-length S protein of MERS-CoV may be detectable if an appropriate assay system is used. Therefore, it would be especially important to investigate the potential of these MERS vaccines to induce virus-enhancing antibodies and harmful immune responses, and to cause immunopathological effects before moving a MERS vaccine candidate into human clinical trials. A lesson should be learned from the development of SARS vaccines – a shift from developing vaccines based on the full-length S protein at the beginning to developing RBD-based vaccines at the end.</p><p>Different from the full-length S protein, RBD of MERS-CoV S protein contains a critical neutralizing domain and lacks immunodominant domains with non-neutralizing epitopes, thus is safe and highly immunogenic to induce potent neutralizing antibodies and protective immunity against MERS-CoV infection. In comparison with other vaccine categories, such as recombinant viruses and viral vectored vaccines, subunit vaccines, including those based on the RBD, maintain higher safety profile due to the absence of viral genetic materials from the infectious viruses. The major conformational neutralizing epitopes in MERS-CoV RBD may attribute to RBD’s ability to induce neutralizing antibodies against both wild-type and mutant viral strains, since a viral strain with mutations in one epitope may still be sensitive to the neutralizing antibodies induced by other epitopes in RBD [<xref rid="CIT0075">75</xref>,<xref rid="CIT0084">84</xref>], demonstrating RBD’s capacity to elicit broad-spectrum neutralizing antibodies and cross-protective immunity. Therefore, similar to RBD-based SARS vaccines [<xref rid="CIT0011">11</xref>,<xref rid="CIT0127">127</xref>–<xref rid="CIT0133">133</xref>], subunit vaccines based on MERS-CoV RBD have the greatest potential for further development as an effective and safe vaccine candidate. It is noted that in addition to RBD, other regions, such as N-terminal domain, in S1 fragment of MERS-CoV may also possess some neutralizing epitopes [<xref rid="CIT0119">119</xref>,<xref rid="CIT0134">134</xref>]. Thus, combining RBD and S1 N-terminal domain in a subunit vaccine may result in synergistic effect in inducing broadly cross-neutralizing antibodies against divergent MERS-CoV strains.</p><p>At present, two full-length MERS-CoV S candidate vaccines, one based on MVA and the other on DNA, have been scheduled for clinical trials [<xref rid="CIT0098">98</xref>,<xref rid="CIT0099">99</xref>]. With the continual increase and extensive research of promising MERS vaccines in preclinical studies, more and more candidates with high efficacy and strong safety should be pushed forward to clinical trials for prevention of MERS-CoV infection.</p></sec><sec id="S0008"><title>Five-year view</title><p>In the next 5 years, more robust, affordable small animal models should be developed to help evaluate the efficacy of MERS vaccines. Comprehensive studies on the efficacy and safety of MERS vaccines are expected. Since MERS-CoV RBD-based subunit vaccines induce strong immune responses and neutralizing antibodies and maintain the highest safety profile, such vaccines are expected to increase in number, and with investment from government and Big Pharma, it is further expected that such vaccines will be brought to clinical trials in an expeditious manner and, upon approval, be used for preventing MERS-CoV infection in humans and for building biodefense stockpiles.</p></sec><sec id="S0009"><title>Key issues</title><p><list list-type="bullet"><list-item><p>Since its first identification in Saudi Arabia in 2012, MERS-CoV has infected at least 1638 persons worldwide, including 587 deaths, as of 2 February 2016, with Saudi Arabia and South Korea having the first and second largest MERS cases, respectively.</p></list-item><list-item><p>MERS-CoV uses bats and dromedary camels as the most likely natural reservoirs and intermediate transmission hosts. Human-to-human transmission has been confirmed. Therefore, as a newly added Category C Priority Pathogen, MERS-CoV poses a threat to public health and global safety, highlighting the importance of developing effective and safe MERS vaccines.</p></list-item><list-item><p>Among the four major structural proteins of MERS-CoV, S protein is the most important in viral pathogenesis. MERS-CoV depends on the S protein (S1 and S2 subunits) to bind the cellular receptor DPP4 through the RBD, followed by mediating MERS-CoV entry into target cells. As such, the viral S protein and RBD are major vaccine targets.</p></list-item><list-item><p>Similar to MERS-CoV infection, MERS vaccines can trigger antigen-specific humoral, mucosal, and/or cellular immune responses. While cellular immune responses might be required to clear or kill virus, humoral immune responses, particularly neutralizing antibodies, play critical roles in protecting against MERS-CoV infection.</p></list-item><list-item><p>Several animal models, including small animal models expressing hDPP4 receptor, have been developed to evaluate the efficacy of MERS vaccines.</p></list-item><list-item><p>No MERS vaccines are available for human use. MERS vaccines under development are in preclinical stages, some of which are scheduled for human clinical trials. These vaccines are categorized as recombinant virus, viral vectors, nanoparticles, DNAs, DNAs/proteins, and subunit vaccines, the majority of which are based on the viral S protein.</p></list-item><list-item><p>In addition to the major neutralizing epitopes in RBD, the full-length S protein also contains some immunodominant domains with non-neutralizing epitopes that can induce non-neutralizing antibodies, some of which may mediate enhancement of viral infection or harmful immune responses, or cause immunopathological effects, as those induced by the full-length S protein of SARS-CoV.</p></list-item><list-item><p>The RBD in the S1 subunit of MERS-CoV S protein contains major neutralizing epitopes and lacks immunodominant domains with non-neutralizing epitopes, thus having much less risk to induce virus-enhancing antibody or harmful immune responses and better potential than full-length S protein to be developed as an effective and safe MERS vaccine.</p></list-item></list></p></sec></body><back><sec id="S0010"><title>Financial & competing interests disclosure</title><p>This study was supported by the grant from the NIAID of the NIH (AI109094) and intramural funds from the New York Blood Center (NYB000348). 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