A25 Phylogenetic analysis of the nucleocapsid and RNA-dependent RNA polymerase fragments of the first imported case of middle east respiratory syndrome coronavirus (MERS-CoV) infection in the Philippines from Saudi Arabia, February 2015
Identifieur interne : 001190 ( Pmc/Corpus ); précédent : 001189; suivant : 001191A25 Phylogenetic analysis of the nucleocapsid and RNA-dependent RNA polymerase fragments of the first imported case of middle east respiratory syndrome coronavirus (MERS-CoV) infection in the Philippines from Saudi Arabia, February 2015
Auteurs : I. A. P. Medado ; J. R. C. Orbina ; N. T. M. Yabut ; L. L. M. Dancel ; T. C. Tan ; M. A. U. Igoy ; A. M. R. Mojica ; I. C. Lirio ; A. C. Ablola ; B. C. Mateo ; M. A. J. Biol ; A. D. Nicolasora ; H. L. E. Morito ; K. I. M. Cruz ; C. M. F. Roldan ; P. B. Medina ; E. S. Mercado ; C. S. Demetria ; R. J. Capistrano ; S. P. LupisanSource :
- Virus Evolution [ 2057-1577 ] ; 2017.
Url:
DOI: 10.1093/ve/vew036.024
PubMed: 28845258
PubMed Central: 5565944
Links to Exploration step
PMC:5565944Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A25 Phylogenetic analysis of the nucleocapsid and RNA-dependent RNA polymerase fragments of the first imported case of middle east respiratory syndrome coronavirus (MERS-CoV) infection in the Philippines from Saudi Arabia, February 2015</title><author><name sortKey="Medado, I A P" sort="Medado, I A P" uniqKey="Medado I" first="I. A. P." last="Medado">I. A. P. Medado</name></author><author><name sortKey="Orbina, J R C" sort="Orbina, J R C" uniqKey="Orbina J" first="J. R. C." last="Orbina">J. R. C. Orbina</name></author><author><name sortKey="Yabut, N T M" sort="Yabut, N T M" uniqKey="Yabut N" first="N. T. M." last="Yabut">N. T. M. Yabut</name></author><author><name sortKey="Dancel, L L M" sort="Dancel, L L M" uniqKey="Dancel L" first="L. L. M." last="Dancel">L. L. M. Dancel</name></author><author><name sortKey="Tan, T C" sort="Tan, T C" uniqKey="Tan T" first="T. C." last="Tan">T. C. Tan</name></author><author><name sortKey="Igoy, M A U" sort="Igoy, M A U" uniqKey="Igoy M" first="M. A. U." last="Igoy">M. A. U. Igoy</name></author><author><name sortKey="Mojica, A M R" sort="Mojica, A M R" uniqKey="Mojica A" first="A. M. R." last="Mojica">A. M. R. Mojica</name></author><author><name sortKey="Lirio, I C" sort="Lirio, I C" uniqKey="Lirio I" first="I. C." last="Lirio">I. C. Lirio</name></author><author><name sortKey="Ablola, A C" sort="Ablola, A C" uniqKey="Ablola A" first="A. C." last="Ablola">A. C. Ablola</name></author><author><name sortKey="Mateo, B C" sort="Mateo, B C" uniqKey="Mateo B" first="B. C." last="Mateo">B. C. Mateo</name></author><author><name sortKey="Biol, M A J" sort="Biol, M A J" uniqKey="Biol M" first="M. A. J." last="Biol">M. A. J. Biol</name></author><author><name sortKey="Nicolasora, A D" sort="Nicolasora, A D" uniqKey="Nicolasora A" first="A. D." last="Nicolasora">A. D. Nicolasora</name></author><author><name sortKey="Morito, H L E" sort="Morito, H L E" uniqKey="Morito H" first="H. L. E." last="Morito">H. L. E. Morito</name></author><author><name sortKey="Cruz, K I M" sort="Cruz, K I M" uniqKey="Cruz K" first="K. I. M." last="Cruz">K. I. M. Cruz</name></author><author><name sortKey="Roldan, C M F" sort="Roldan, C M F" uniqKey="Roldan C" first="C. M. F." last="Roldan">C. M. F. Roldan</name></author><author><name sortKey="Medina, P B" sort="Medina, P B" uniqKey="Medina P" first="P. B." last="Medina">P. B. Medina</name></author><author><name sortKey="Mercado, E S" sort="Mercado, E S" uniqKey="Mercado E" first="E. S." last="Mercado">E. S. Mercado</name></author><author><name sortKey="Demetria, C S" sort="Demetria, C S" uniqKey="Demetria C" first="C. S." last="Demetria">C. S. Demetria</name></author><author><name sortKey="Capistrano, R J" sort="Capistrano, R J" uniqKey="Capistrano R" first="R. J." last="Capistrano">R. J. Capistrano</name></author><author><name sortKey="Lupisan, S P" sort="Lupisan, S P" uniqKey="Lupisan S" first="S. P." last="Lupisan">S. P. Lupisan</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28845258</idno><idno type="pmc">5565944</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5565944</idno><idno type="RBID">PMC:5565944</idno><idno type="doi">10.1093/ve/vew036.024</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">001190</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001190</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A25 Phylogenetic analysis of the nucleocapsid and RNA-dependent RNA polymerase fragments of the first imported case of middle east respiratory syndrome coronavirus (MERS-CoV) infection in the Philippines from Saudi Arabia, February 2015</title><author><name sortKey="Medado, I A P" sort="Medado, I A P" uniqKey="Medado I" first="I. A. P." last="Medado">I. A. P. Medado</name></author><author><name sortKey="Orbina, J R C" sort="Orbina, J R C" uniqKey="Orbina J" first="J. R. C." last="Orbina">J. R. C. Orbina</name></author><author><name sortKey="Yabut, N T M" sort="Yabut, N T M" uniqKey="Yabut N" first="N. T. M." last="Yabut">N. T. M. Yabut</name></author><author><name sortKey="Dancel, L L M" sort="Dancel, L L M" uniqKey="Dancel L" first="L. L. M." last="Dancel">L. L. M. Dancel</name></author><author><name sortKey="Tan, T C" sort="Tan, T C" uniqKey="Tan T" first="T. C." last="Tan">T. C. Tan</name></author><author><name sortKey="Igoy, M A U" sort="Igoy, M A U" uniqKey="Igoy M" first="M. A. U." last="Igoy">M. A. U. Igoy</name></author><author><name sortKey="Mojica, A M R" sort="Mojica, A M R" uniqKey="Mojica A" first="A. M. R." last="Mojica">A. M. R. Mojica</name></author><author><name sortKey="Lirio, I C" sort="Lirio, I C" uniqKey="Lirio I" first="I. C." last="Lirio">I. C. Lirio</name></author><author><name sortKey="Ablola, A C" sort="Ablola, A C" uniqKey="Ablola A" first="A. C." last="Ablola">A. C. Ablola</name></author><author><name sortKey="Mateo, B C" sort="Mateo, B C" uniqKey="Mateo B" first="B. C." last="Mateo">B. C. Mateo</name></author><author><name sortKey="Biol, M A J" sort="Biol, M A J" uniqKey="Biol M" first="M. A. J." last="Biol">M. A. J. Biol</name></author><author><name sortKey="Nicolasora, A D" sort="Nicolasora, A D" uniqKey="Nicolasora A" first="A. D." last="Nicolasora">A. D. Nicolasora</name></author><author><name sortKey="Morito, H L E" sort="Morito, H L E" uniqKey="Morito H" first="H. L. E." last="Morito">H. L. E. Morito</name></author><author><name sortKey="Cruz, K I M" sort="Cruz, K I M" uniqKey="Cruz K" first="K. I. M." last="Cruz">K. I. M. Cruz</name></author><author><name sortKey="Roldan, C M F" sort="Roldan, C M F" uniqKey="Roldan C" first="C. M. F." last="Roldan">C. M. F. Roldan</name></author><author><name sortKey="Medina, P B" sort="Medina, P B" uniqKey="Medina P" first="P. B." last="Medina">P. B. Medina</name></author><author><name sortKey="Mercado, E S" sort="Mercado, E S" uniqKey="Mercado E" first="E. S." last="Mercado">E. S. Mercado</name></author><author><name sortKey="Demetria, C S" sort="Demetria, C S" uniqKey="Demetria C" first="C. S." last="Demetria">C. S. Demetria</name></author><author><name sortKey="Capistrano, R J" sort="Capistrano, R J" uniqKey="Capistrano R" first="R. J." last="Capistrano">R. J. Capistrano</name></author><author><name sortKey="Lupisan, S P" sort="Lupisan, S P" uniqKey="Lupisan S" first="S. P." last="Lupisan">S. P. Lupisan</name></author></analytic><series><title level="j">Virus Evolution</title><idno type="eISSN">2057-1577</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Virus Evol</journal-id><journal-id journal-id-type="iso-abbrev">Virus Evol</journal-id><journal-id journal-id-type="publisher-id">vevolu</journal-id><journal-title-group><journal-title>Virus Evolution</journal-title></journal-title-group><issn pub-type="epub">2057-1577</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28845258</article-id><article-id pub-id-type="pmc">5565944</article-id><article-id pub-id-type="doi">10.1093/ve/vew036.024</article-id><article-id pub-id-type="publisher-id">vew036.024</article-id><article-categories><subj-group subj-group-type="heading"><subject>Abstract Overview</subject><subj-group subj-group-type="category-toc-heading"><subject>21st International BioInformatics Workshop on Virus Evolution and Molecular Epidemiology</subject></subj-group></subj-group></article-categories><title-group><article-title>A25 Phylogenetic analysis of the nucleocapsid and RNA-dependent RNA polymerase fragments of the first imported case of middle east respiratory syndrome coronavirus (MERS-CoV) infection in the Philippines from Saudi Arabia, February 2015</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Medado</surname><given-names>I.A.P.</given-names></name><xref ref-type="aff" rid="AFF200">1</xref><aff id="AFF200"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Orbina</surname><given-names>J.R.C.</given-names></name><xref ref-type="aff" rid="AFF201">1</xref><aff id="AFF201"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Yabut</surname><given-names>N.T.M.</given-names></name><xref ref-type="aff" rid="AFF202">1</xref><aff id="AFF202"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Dancel</surname><given-names>L.L.M.</given-names></name><xref ref-type="aff" rid="AFF203">1</xref><aff id="AFF203"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Tan</surname><given-names>T.C.</given-names></name><xref ref-type="aff" rid="AFF204">1</xref><aff id="AFF204"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Igoy</surname><given-names>M.A.U.</given-names></name><xref ref-type="aff" rid="AFF205">1</xref><aff id="AFF205"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Mojica</surname><given-names>A.M.R.</given-names></name><xref ref-type="aff" rid="AFF206">1</xref><aff id="AFF206"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Lirio</surname><given-names>I.C.</given-names></name><xref ref-type="aff" rid="AFF207">1</xref><aff id="AFF207"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Ablola</surname><given-names>A.C.</given-names></name><xref ref-type="aff" rid="AFF208">1</xref><aff id="AFF208"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Mateo</surname><given-names>B.C.</given-names></name><xref ref-type="aff" rid="AFF209">1</xref><aff id="AFF209"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Biol</surname><given-names>M.A.J.</given-names></name><xref ref-type="aff" rid="AFF210">1</xref><aff id="AFF210"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Nicolasora</surname><given-names>A.D.</given-names></name><xref ref-type="aff" rid="AFF211">1</xref><aff id="AFF211"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Morito</surname><given-names>H.L.E.</given-names></name><xref ref-type="aff" rid="AFF212">1</xref><aff id="AFF212"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Cruz</surname><given-names>K.I.M.</given-names></name><xref ref-type="aff" rid="AFF213">1</xref><aff id="AFF213"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Roldan</surname><given-names>C.M.F.</given-names></name><xref ref-type="aff" rid="AFF214">1</xref><aff id="AFF214"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Medina</surname><given-names>P.B.</given-names></name><xref ref-type="aff" rid="AFF215">2</xref><aff id="AFF215"><label>2</label>Special Pathogens Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Mercado</surname><given-names>E.S.</given-names></name><xref ref-type="aff" rid="AFF216">1</xref><aff id="AFF216"><label>1</label>Molecular Biology Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Demetria</surname><given-names>C.S.</given-names></name><xref ref-type="aff" rid="AFF217">2</xref><aff id="AFF217"><label>2</label>Special Pathogens Laboratory, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Capistrano</surname><given-names>R.J.</given-names></name><xref ref-type="aff" rid="AFF218">3</xref><aff id="AFF218"><label>3</label>Surveillance and Response Unit, Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib><contrib contrib-type="author"><name><surname>Lupisan</surname><given-names>S.P.</given-names></name><xref ref-type="aff" rid="AFF219">4</xref><aff id="AFF219"><label>4</label>Research Institute for Tropical Medicine - Department of Health, Philippines</aff></contrib></contrib-group><pub-date pub-type="collection"><month>3</month><year>2017</year></pub-date><pub-date pub-type="epub" iso-8601-date="2017-03-05"><day>05</day><month>3</month><year>2017</year></pub-date><pub-date pub-type="pmc-release"><day>05</day><month>3</month><year>2017</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>3</volume><issue>Suppl 1</issue><elocation-id>vew036.024</elocation-id><permissions><copyright-statement>© Published by Oxford University Press.</copyright-statement><copyright-year>2017</copyright-year><license license-type="cc-by-nc" xlink:href="http://creativecommons.org/licenses/by-nc/4.0/"><license-p>This is an Open Access publication distributed under the terms of the Creative Commons Attribution Non-Commercial License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/4.0/">http://creativecommons.org/licenses/by-nc/4.0/</ext-link>), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com</license-p></license></permissions><self-uri xlink:href="vew036.024.pdf"></self-uri><counts><page-count count="2"></page-count></counts></article-meta></front><body><p>We report the first laboratory-confirmed case of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) infection from a patient returning to the Philippines from the Kingdom of Saudi Arabia (KSA). MERS-CoV was first identified in 2012 circulating in Middle Eastern countries with outbreaks occurring in KSA, the United Arab Emirates (UAE), and South Korea, plus sporadic imported cases in at least 20 other countries. The Philippines is at risk for MERS-CoV transmission from frequent travelers, such as overseas Filipino workers and Hajj pilgrims, coming from Middle Eastern countries. Throat swabs, sputum samples, and a rectal swab were collected from the index case within 13 to 22 days after the onset of symptoms. MERS-CoV testing was performed using a real-time reverse transcription polymerase chain reaction (RT-qPCR) screening assay targeting regions upstream of the envelope gene (upE) and the nucleocapsid gene (N2), a confirmatory RT-qPCR assay targeting regions within the open reading frame 1a gene (ORF1a) and another region of the N gene (N3), and Sanger sequencing of regions of the N and RNA-dependent polymerase (RdRp) genes. The index case tested weakly positive for MERS-CoV in a sputum sample until day 19 of illness. Sequences of the N and RdRp gene regions reveal 100 and 99% similarity with MERS-CoV sequences obtained in KSA and UAE, respectively, confirming that the infection originated from Middle Eastern strains. Two unique synonymous/silent mutations (T15259A and T15265C) were identified in the RdRp sequence fragments. Whole genome sequencing of the strain may identify other mutations across the genome and determine the most probable origin of the strain.</p></body></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001190 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 001190 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé= PMC:5565944
|texte= A25 Phylogenetic analysis of the nucleocapsid and RNA-dependent RNA polymerase fragments of the first imported case of middle east respiratory syndrome coronavirus (MERS-CoV) infection in the Philippines from Saudi Arabia, February 2015
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:28845258" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |