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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Early endonuclease-mediated evasion of RNA sensing ensures efficient coronavirus replication</title><author><name sortKey="Kindler, Eveline" sort="Kindler, Eveline" uniqKey="Kindler E" first="Eveline" last="Kindler">Eveline Kindler</name><affiliation><nlm:aff id="aff001"><addr-line>Department of Infectious Diseases and Pathobiology, University of Bern, Bern, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Federal Department of Home Affairs, Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Gil Cruz, Cristina" sort="Gil Cruz, Cristina" uniqKey="Gil Cruz C" first="Cristina" last="Gil-Cruz">Cristina Gil-Cruz</name><affiliation><nlm:aff id="aff003"><addr-line>Institute of Immunobiology, Kantonsspital St.Gallen, St.Gallen, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Spanier, Julia" sort="Spanier, Julia" uniqKey="Spanier J" first="Julia" last="Spanier">Julia Spanier</name><affiliation><nlm:aff id="aff004"><addr-line>Institute for Experimental Infection Research, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Helmholtz Centre for Infection Research and the Hannover Medical School, Hannover, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Li, Yize" sort="Li, Yize" uniqKey="Li Y" first="Yize" last="Li">Yize Li</name><affiliation><nlm:aff id="aff005"><addr-line>Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Wilhelm, Jochen" sort="Wilhelm, Jochen" uniqKey="Wilhelm J" first="Jochen" last="Wilhelm">Jochen Wilhelm</name><affiliation><nlm:aff id="aff006"><addr-line>Universities Giessen & Marburg Lung Center (UGMLC), Deutsches Zentrum für Lungenforschung (DZL), Giessen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Rabouw, Huib H" sort="Rabouw, Huib H" uniqKey="Rabouw H" first="Huib H." last="Rabouw">Huib H. Rabouw</name><affiliation><nlm:aff id="aff007"><addr-line>Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands</addr-line></nlm:aff></affiliation></author><author><name sortKey="Zust, Roland" sort="Zust, Roland" uniqKey="Zust R" first="Roland" last="Züst">Roland Züst</name><affiliation><nlm:aff id="aff008"><addr-line>Singapore Immunology Network, Singapore</addr-line></nlm:aff></affiliation></author><author><name sortKey="Hwang, Mihyun" sort="Hwang, Mihyun" uniqKey="Hwang M" first="Mihyun" last="Hwang">Mihyun Hwang</name><affiliation><nlm:aff id="aff009"><addr-line>Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="V Ovski, Philip" sort="V Ovski, Philip" uniqKey="V Ovski P" first="Philip" last="V Ovski">Philip V Ovski</name><affiliation><nlm:aff id="aff001"><addr-line>Department of Infectious Diseases and Pathobiology, University of Bern, Bern, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Federal Department of Home Affairs, Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff010"><addr-line>Graduate School for Biomedical Science, University of Bern, Bern, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Stalder, Hanspeter" sort="Stalder, Hanspeter" uniqKey="Stalder H" first="Hanspeter" last="Stalder">Hanspeter Stalder</name><affiliation><nlm:aff id="aff001"><addr-line>Department of Infectious Diseases and Pathobiology, University of Bern, Bern, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Federal Department of Home Affairs, Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Marti, Sabrina" sort="Marti, Sabrina" uniqKey="Marti S" first="Sabrina" last="Marti">Sabrina Marti</name><affiliation><nlm:aff id="aff001"><addr-line>Department of Infectious Diseases and Pathobiology, University of Bern, Bern, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Federal Department of Home Affairs, Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Habjan, Matthias" sort="Habjan, Matthias" uniqKey="Habjan M" first="Matthias" last="Habjan">Matthias Habjan</name><affiliation><nlm:aff id="aff011"><addr-line>Max-Planck-Institute of Biochemistry, Martinsried, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Cervantes Barragan, Luisa" sort="Cervantes Barragan, Luisa" uniqKey="Cervantes Barragan L" first="Luisa" last="Cervantes-Barragan">Luisa Cervantes-Barragan</name><affiliation><nlm:aff id="aff012"><addr-line>Washington University School of Medicine, St. Louis, MO, USA</addr-line></nlm:aff></affiliation></author><author><name sortKey="Elliot, Ruth" sort="Elliot, Ruth" uniqKey="Elliot R" first="Ruth" last="Elliot">Ruth Elliot</name><affiliation><nlm:aff id="aff005"><addr-line>Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Karl, Nadja" sort="Karl, Nadja" uniqKey="Karl N" first="Nadja" last="Karl">Nadja Karl</name><affiliation><nlm:aff id="aff013"><addr-line>Institute for Medical Virology, Justus-Liebig-University, Giessen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Gaughan, Christina" sort="Gaughan, Christina" uniqKey="Gaughan C" first="Christina" last="Gaughan">Christina Gaughan</name><affiliation><nlm:aff id="aff014"><addr-line>Department of Cancer Biology, Lerner Research Institute, Cleveland, Ohio, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Van Kuppeveld, Frank J M" sort="Van Kuppeveld, Frank J M" uniqKey="Van Kuppeveld F" first="Frank J. M." last="Van Kuppeveld">Frank J. M. Van Kuppeveld</name><affiliation><nlm:aff id="aff007"><addr-line>Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands</addr-line></nlm:aff></affiliation></author><author><name sortKey="Silverman, Robert H" sort="Silverman, Robert H" uniqKey="Silverman R" first="Robert H." last="Silverman">Robert H. Silverman</name><affiliation><nlm:aff id="aff014"><addr-line>Department of Cancer Biology, Lerner Research Institute, Cleveland, Ohio, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Keller, Markus" sort="Keller, Markus" uniqKey="Keller M" first="Markus" last="Keller">Markus Keller</name><affiliation><nlm:aff id="aff015"><addr-line>Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ludewig, Burkhard" sort="Ludewig, Burkhard" uniqKey="Ludewig B" first="Burkhard" last="Ludewig">Burkhard Ludewig</name><affiliation><nlm:aff id="aff003"><addr-line>Institute of Immunobiology, Kantonsspital St.Gallen, St.Gallen, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Bergmann, Cornelia C" sort="Bergmann, Cornelia C" uniqKey="Bergmann C" first="Cornelia C." last="Bergmann">Cornelia C. Bergmann</name><affiliation><nlm:aff id="aff009"><addr-line>Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ziebuhr, John" sort="Ziebuhr, John" uniqKey="Ziebuhr J" first="John" last="Ziebuhr">John Ziebuhr</name><affiliation><nlm:aff id="aff013"><addr-line>Institute for Medical Virology, Justus-Liebig-University, Giessen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Weiss, Susan R" sort="Weiss, Susan R" uniqKey="Weiss S" first="Susan R." last="Weiss">Susan R. Weiss</name><affiliation><nlm:aff id="aff005"><addr-line>Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kalinke, Ulrich" sort="Kalinke, Ulrich" uniqKey="Kalinke U" first="Ulrich" last="Kalinke">Ulrich Kalinke</name><affiliation><nlm:aff id="aff004"><addr-line>Institute for Experimental Infection Research, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Helmholtz Centre for Infection Research and the Hannover Medical School, Hannover, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name><affiliation><nlm:aff id="aff001"><addr-line>Department of Infectious Diseases and Pathobiology, University of Bern, Bern, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Federal Department of Home Affairs, Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28158275</idno><idno type="pmc">5310923</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5310923</idno><idno type="RBID">PMC:5310923</idno><idno type="doi">10.1371/journal.ppat.1006195</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">001100</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001100</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Early endonuclease-mediated evasion of RNA sensing ensures efficient coronavirus replication</title><author><name sortKey="Kindler, Eveline" sort="Kindler, Eveline" uniqKey="Kindler E" first="Eveline" last="Kindler">Eveline Kindler</name><affiliation><nlm:aff id="aff001"><addr-line>Department of Infectious Diseases and Pathobiology, University of Bern, Bern, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Federal Department of Home Affairs, Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Gil Cruz, Cristina" sort="Gil Cruz, Cristina" uniqKey="Gil Cruz C" first="Cristina" last="Gil-Cruz">Cristina Gil-Cruz</name><affiliation><nlm:aff id="aff003"><addr-line>Institute of Immunobiology, Kantonsspital St.Gallen, St.Gallen, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Spanier, Julia" sort="Spanier, Julia" uniqKey="Spanier J" first="Julia" last="Spanier">Julia Spanier</name><affiliation><nlm:aff id="aff004"><addr-line>Institute for Experimental Infection Research, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Helmholtz Centre for Infection Research and the Hannover Medical School, Hannover, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Li, Yize" sort="Li, Yize" uniqKey="Li Y" first="Yize" last="Li">Yize Li</name><affiliation><nlm:aff id="aff005"><addr-line>Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Wilhelm, Jochen" sort="Wilhelm, Jochen" uniqKey="Wilhelm J" first="Jochen" last="Wilhelm">Jochen Wilhelm</name><affiliation><nlm:aff id="aff006"><addr-line>Universities Giessen & Marburg Lung Center (UGMLC), Deutsches Zentrum für Lungenforschung (DZL), Giessen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Rabouw, Huib H" sort="Rabouw, Huib H" uniqKey="Rabouw H" first="Huib H." last="Rabouw">Huib H. Rabouw</name><affiliation><nlm:aff id="aff007"><addr-line>Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands</addr-line></nlm:aff></affiliation></author><author><name sortKey="Zust, Roland" sort="Zust, Roland" uniqKey="Zust R" first="Roland" last="Züst">Roland Züst</name><affiliation><nlm:aff id="aff008"><addr-line>Singapore Immunology Network, Singapore</addr-line></nlm:aff></affiliation></author><author><name sortKey="Hwang, Mihyun" sort="Hwang, Mihyun" uniqKey="Hwang M" first="Mihyun" last="Hwang">Mihyun Hwang</name><affiliation><nlm:aff id="aff009"><addr-line>Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="V Ovski, Philip" sort="V Ovski, Philip" uniqKey="V Ovski P" first="Philip" last="V Ovski">Philip V Ovski</name><affiliation><nlm:aff id="aff001"><addr-line>Department of Infectious Diseases and Pathobiology, University of Bern, Bern, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Federal Department of Home Affairs, Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff010"><addr-line>Graduate School for Biomedical Science, University of Bern, Bern, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Stalder, Hanspeter" sort="Stalder, Hanspeter" uniqKey="Stalder H" first="Hanspeter" last="Stalder">Hanspeter Stalder</name><affiliation><nlm:aff id="aff001"><addr-line>Department of Infectious Diseases and Pathobiology, University of Bern, Bern, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Federal Department of Home Affairs, Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Marti, Sabrina" sort="Marti, Sabrina" uniqKey="Marti S" first="Sabrina" last="Marti">Sabrina Marti</name><affiliation><nlm:aff id="aff001"><addr-line>Department of Infectious Diseases and Pathobiology, University of Bern, Bern, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Federal Department of Home Affairs, Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Habjan, Matthias" sort="Habjan, Matthias" uniqKey="Habjan M" first="Matthias" last="Habjan">Matthias Habjan</name><affiliation><nlm:aff id="aff011"><addr-line>Max-Planck-Institute of Biochemistry, Martinsried, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Cervantes Barragan, Luisa" sort="Cervantes Barragan, Luisa" uniqKey="Cervantes Barragan L" first="Luisa" last="Cervantes-Barragan">Luisa Cervantes-Barragan</name><affiliation><nlm:aff id="aff012"><addr-line>Washington University School of Medicine, St. Louis, MO, USA</addr-line></nlm:aff></affiliation></author><author><name sortKey="Elliot, Ruth" sort="Elliot, Ruth" uniqKey="Elliot R" first="Ruth" last="Elliot">Ruth Elliot</name><affiliation><nlm:aff id="aff005"><addr-line>Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Karl, Nadja" sort="Karl, Nadja" uniqKey="Karl N" first="Nadja" last="Karl">Nadja Karl</name><affiliation><nlm:aff id="aff013"><addr-line>Institute for Medical Virology, Justus-Liebig-University, Giessen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Gaughan, Christina" sort="Gaughan, Christina" uniqKey="Gaughan C" first="Christina" last="Gaughan">Christina Gaughan</name><affiliation><nlm:aff id="aff014"><addr-line>Department of Cancer Biology, Lerner Research Institute, Cleveland, Ohio, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Van Kuppeveld, Frank J M" sort="Van Kuppeveld, Frank J M" uniqKey="Van Kuppeveld F" first="Frank J. M." last="Van Kuppeveld">Frank J. M. Van Kuppeveld</name><affiliation><nlm:aff id="aff007"><addr-line>Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands</addr-line></nlm:aff></affiliation></author><author><name sortKey="Silverman, Robert H" sort="Silverman, Robert H" uniqKey="Silverman R" first="Robert H." last="Silverman">Robert H. Silverman</name><affiliation><nlm:aff id="aff014"><addr-line>Department of Cancer Biology, Lerner Research Institute, Cleveland, Ohio, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Keller, Markus" sort="Keller, Markus" uniqKey="Keller M" first="Markus" last="Keller">Markus Keller</name><affiliation><nlm:aff id="aff015"><addr-line>Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ludewig, Burkhard" sort="Ludewig, Burkhard" uniqKey="Ludewig B" first="Burkhard" last="Ludewig">Burkhard Ludewig</name><affiliation><nlm:aff id="aff003"><addr-line>Institute of Immunobiology, Kantonsspital St.Gallen, St.Gallen, Switzerland</addr-line></nlm:aff></affiliation></author><author><name sortKey="Bergmann, Cornelia C" sort="Bergmann, Cornelia C" uniqKey="Bergmann C" first="Cornelia C." last="Bergmann">Cornelia C. Bergmann</name><affiliation><nlm:aff id="aff009"><addr-line>Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ziebuhr, John" sort="Ziebuhr, John" uniqKey="Ziebuhr J" first="John" last="Ziebuhr">John Ziebuhr</name><affiliation><nlm:aff id="aff013"><addr-line>Institute for Medical Virology, Justus-Liebig-University, Giessen, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Weiss, Susan R" sort="Weiss, Susan R" uniqKey="Weiss S" first="Susan R." last="Weiss">Susan R. Weiss</name><affiliation><nlm:aff id="aff005"><addr-line>Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kalinke, Ulrich" sort="Kalinke, Ulrich" uniqKey="Kalinke U" first="Ulrich" last="Kalinke">Ulrich Kalinke</name><affiliation><nlm:aff id="aff004"><addr-line>Institute for Experimental Infection Research, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Helmholtz Centre for Infection Research and the Hannover Medical School, Hannover, Germany</addr-line></nlm:aff></affiliation></author><author><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name><affiliation><nlm:aff id="aff001"><addr-line>Department of Infectious Diseases and Pathobiology, University of Bern, Bern, Switzerland</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff002"><addr-line>Federal Department of Home Affairs, Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">PLoS Pathogens</title><idno type="ISSN">1553-7366</idno><idno type="eISSN">1553-7374</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Coronaviruses are of veterinary and medical importance and include highly pathogenic zoonotic viruses, such as SARS-CoV and MERS-CoV. They are known to efficiently evade early innate immune responses, manifesting in almost negligible expression of type-I interferons (IFN-I). This evasion strategy suggests an evolutionary conserved viral function that has evolved to prevent RNA-based sensing of infection in vertebrate hosts. Here we show that the coronavirus endonuclease (EndoU) activity is key to prevent early induction of double-stranded RNA (dsRNA) host cell responses. Replication of EndoU-deficient coronaviruses is greatly attenuated <italic>in vivo</italic> and severely restricted in primary cells even during the early phase of the infection. In macrophages we found immediate induction of IFN-I expression and RNase L-mediated breakdown of ribosomal RNA. Accordingly, EndoU-deficient viruses can retain replication only in cells that are deficient in IFN-I expression or sensing, and in cells lacking both RNase L and PKR. Collectively our results demonstrate that the coronavirus EndoU efficiently prevents simultaneous activation of host cell dsRNA sensors, such as Mda5, OAS and PKR. The localization of the EndoU activity at the site of viral RNA synthesis–within the replicase complex—suggests that coronaviruses have evolved a viral RNA decay pathway to evade early innate and intrinsic antiviral host cell responses.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Tenoever, Br" uniqKey="Tenoever B">BR tenOever</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schneider, Wm" uniqKey="Schneider W">WM Schneider</name></author><author><name sortKey="Chevillotte, Md" uniqKey="Chevillotte M">MD Chevillotte</name></author><author><name sortKey="Rice, Cm" uniqKey="Rice C">CM Rice</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Channappanavar, R" uniqKey="Channappanavar R">R Channappanavar</name></author><author><name sortKey="Fehr, Ar" uniqKey="Fehr A">AR Fehr</name></author><author><name sortKey="Vijay, R" uniqKey="Vijay R">R 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS Pathog</journal-id><journal-id journal-id-type="iso-abbrev">PLoS Pathog</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plospath</journal-id><journal-title-group><journal-title>PLoS Pathogens</journal-title></journal-title-group><issn pub-type="ppub">1553-7366</issn><issn pub-type="epub">1553-7374</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28158275</article-id><article-id pub-id-type="pmc">5310923</article-id><article-id pub-id-type="doi">10.1371/journal.ppat.1006195</article-id><article-id pub-id-type="publisher-id">PPATHOGENS-D-16-01691</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cellular Types</subject><subj-group><subject>Animal Cells</subject><subj-group><subject>Blood Cells</subject><subj-group><subject>White Blood Cells</subject><subj-group><subject>Macrophages</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cellular Types</subject><subj-group><subject>Animal Cells</subject><subj-group><subject>Immune Cells</subject><subj-group><subject>White Blood Cells</subject><subj-group><subject>Macrophages</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Immunology</subject><subj-group><subject>Immune Cells</subject><subj-group><subject>White Blood Cells</subject><subj-group><subject>Macrophages</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Immunology</subject><subj-group><subject>Immune Cells</subject><subj-group><subject>White Blood Cells</subject><subj-group><subject>Macrophages</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Microbiology</subject><subj-group><subject>Virology</subject><subj-group><subject>Viral Replication</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and life sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Proteins</subject><subj-group><subject>DNA-binding proteins</subject><subj-group><subject>Nucleases</subject><subj-group><subject>Ribonucleases</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Enzymology</subject><subj-group><subject>Enzymes</subject><subj-group><subject>Hydrolases</subject><subj-group><subject>Nucleases</subject><subj-group><subject>Ribonucleases</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Proteins</subject><subj-group><subject>Enzymes</subject><subj-group><subject>Hydrolases</subject><subj-group><subject>Nucleases</subject><subj-group><subject>Ribonucleases</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and life sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Viruses</subject><subj-group><subject>RNA viruses</subject><subj-group><subject>Coronaviruses</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Microbiology</subject><subj-group><subject>Medical Microbiology</subject><subj-group><subject>Microbial Pathogens</subject><subj-group><subject>Viral Pathogens</subject><subj-group><subject>Coronaviruses</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Pathology and Laboratory Medicine</subject><subj-group><subject>Pathogens</subject><subj-group><subject>Microbial Pathogens</subject><subj-group><subject>Viral Pathogens</subject><subj-group><subject>Coronaviruses</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Viruses</subject><subj-group><subject>Viral Pathogens</subject><subj-group><subject>Coronaviruses</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and analysis methods</subject><subj-group><subject>Chemical synthesis</subject><subj-group><subject>Biosynthetic techniques</subject><subj-group><subject>Nucleic acid synthesis</subject><subj-group><subject>RNA synthesis</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and life sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Nucleic acids</subject><subj-group><subject>RNA</subject><subj-group><subject>RNA synthesis</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Microbiology</subject><subj-group><subject>Virology</subject><subj-group><subject>Viral Transmission and Infection</subject><subj-group><subject>Host Cells</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and life sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Nucleic acids</subject><subj-group><subject>RNA</subject><subj-group><subject>Non-coding RNA</subject><subj-group><subject>Ribosomal RNA</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and life sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Ribosomes</subject><subj-group><subject>Ribosomal RNA</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and life sciences</subject><subj-group><subject>Cell biology</subject><subj-group><subject>Cellular structures and organelles</subject><subj-group><subject>Ribosomes</subject><subj-group><subject>Ribosomal RNA</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Biological Cultures</subject><subj-group><subject>Cell Lines</subject><subj-group><subject>L929 Cells</subject></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Early endonuclease-mediated evasion of RNA sensing ensures efficient coronavirus replication</article-title><alt-title alt-title-type="running-head">Viral endonuclease and innate immunity</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Kindler</surname><given-names>Eveline</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Gil-Cruz</surname><given-names>Cristina</given-names></name><xref ref-type="aff" rid="aff003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Spanier</surname><given-names>Julia</given-names></name><xref ref-type="aff" rid="aff004"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Yize</given-names></name><xref ref-type="aff" rid="aff005"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Wilhelm</surname><given-names>Jochen</given-names></name><xref ref-type="aff" rid="aff006"><sup>6</sup></xref></contrib><contrib contrib-type="author"><name><surname>Rabouw</surname><given-names>Huib H.</given-names></name><xref ref-type="aff" rid="aff007"><sup>7</sup></xref></contrib><contrib contrib-type="author"><name><surname>Züst</surname><given-names>Roland</given-names></name><xref ref-type="aff" rid="aff008"><sup>8</sup></xref></contrib><contrib contrib-type="author"><contrib-id authenticated="true" contrib-id-type="orcid">http://orcid.org/0000-0003-2639-0265</contrib-id><name><surname>Hwang</surname><given-names>Mihyun</given-names></name><xref ref-type="aff" rid="aff009"><sup>9</sup></xref></contrib><contrib contrib-type="author"><contrib-id authenticated="true" contrib-id-type="orcid">http://orcid.org/0000-0002-8366-1220</contrib-id><name><surname>V’kovski</surname><given-names>Philip</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref><xref ref-type="aff" rid="aff010"><sup>10</sup></xref></contrib><contrib contrib-type="author"><contrib-id authenticated="true" contrib-id-type="orcid">http://orcid.org/0000-0003-0763-6469</contrib-id><name><surname>Stalder</surname><given-names>Hanspeter</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Marti</surname><given-names>Sabrina</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Habjan</surname><given-names>Matthias</given-names></name><xref ref-type="aff" rid="aff011"><sup>11</sup></xref></contrib><contrib contrib-type="author"><name><surname>Cervantes-Barragan</surname><given-names>Luisa</given-names></name><xref ref-type="aff" rid="aff012"><sup>12</sup></xref></contrib><contrib contrib-type="author"><name><surname>Elliot</surname><given-names>Ruth</given-names></name><xref ref-type="aff" rid="aff005"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Karl</surname><given-names>Nadja</given-names></name><xref ref-type="aff" rid="aff013"><sup>13</sup></xref></contrib><contrib contrib-type="author"><name><surname>Gaughan</surname><given-names>Christina</given-names></name><xref ref-type="aff" rid="aff014"><sup>14</sup></xref></contrib><contrib contrib-type="author"><contrib-id authenticated="true" contrib-id-type="orcid">http://orcid.org/0000-0001-5800-749X</contrib-id><name><surname>van Kuppeveld</surname><given-names>Frank J. M.</given-names></name><xref ref-type="aff" rid="aff007"><sup>7</sup></xref></contrib><contrib contrib-type="author"><name><surname>Silverman</surname><given-names>Robert H.</given-names></name><xref ref-type="aff" rid="aff014"><sup>14</sup></xref></contrib><contrib contrib-type="author"><name><surname>Keller</surname><given-names>Markus</given-names></name><xref ref-type="aff" rid="aff015"><sup>15</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ludewig</surname><given-names>Burkhard</given-names></name><xref ref-type="aff" rid="aff003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Bergmann</surname><given-names>Cornelia C.</given-names></name><xref ref-type="aff" rid="aff009"><sup>9</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ziebuhr</surname><given-names>John</given-names></name><xref ref-type="aff" rid="aff013"><sup>13</sup></xref></contrib><contrib contrib-type="author"><contrib-id authenticated="true" contrib-id-type="orcid">http://orcid.org/0000-0002-8155-4528</contrib-id><name><surname>Weiss</surname><given-names>Susan R.</given-names></name><xref ref-type="aff" rid="aff005"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kalinke</surname><given-names>Ulrich</given-names></name><xref ref-type="aff" rid="aff004"><sup>4</sup></xref></contrib><contrib contrib-type="author"><contrib-id authenticated="true" contrib-id-type="orcid">http://orcid.org/0000-0002-5783-0887</contrib-id><name><surname>Thiel</surname><given-names>Volker</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref><xref ref-type="corresp" rid="cor001">*</xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Department of Infectious Diseases and Pathobiology, University of Bern, Bern, Switzerland</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Federal Department of Home Affairs, Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland</addr-line></aff><aff id="aff003"><label>3</label><addr-line>Institute of Immunobiology, Kantonsspital St.Gallen, St.Gallen, Switzerland</addr-line></aff><aff id="aff004"><label>4</label><addr-line>Institute for Experimental Infection Research, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Helmholtz Centre for Infection Research and the Hannover Medical School, Hannover, Germany</addr-line></aff><aff id="aff005"><label>5</label><addr-line>Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States of America</addr-line></aff><aff id="aff006"><label>6</label><addr-line>Universities Giessen & Marburg Lung Center (UGMLC), Deutsches Zentrum für Lungenforschung (DZL), Giessen, Germany</addr-line></aff><aff id="aff007"><label>7</label><addr-line>Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands</addr-line></aff><aff id="aff008"><label>8</label><addr-line>Singapore Immunology Network, Singapore</addr-line></aff><aff id="aff009"><label>9</label><addr-line>Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, United States of America</addr-line></aff><aff id="aff010"><label>10</label><addr-line>Graduate School for Biomedical Science, University of Bern, Bern, Switzerland</addr-line></aff><aff id="aff011"><label>11</label><addr-line>Max-Planck-Institute of Biochemistry, Martinsried, Germany</addr-line></aff><aff id="aff012"><label>12</label><addr-line>Washington University School of Medicine, St. Louis, MO, USA</addr-line></aff><aff id="aff013"><label>13</label><addr-line>Institute for Medical Virology, Justus-Liebig-University, Giessen, Germany</addr-line></aff><aff id="aff014"><label>14</label><addr-line>Department of Cancer Biology, Lerner Research Institute, Cleveland, Ohio, United States of America</addr-line></aff><aff id="aff015"><label>15</label><addr-line>Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Perlman</surname><given-names>Stanley</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>University of Iowa, UNITED STATES</addr-line></aff><author-notes><fn fn-type="COI-statement" id="coi001"><p>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p><list list-type="simple"><list-item><p><bold>Conceptualization:</bold> EK RZ LCB BL VT.</p></list-item><list-item><p><bold>Formal analysis:</bold> EK JW MHw HHR PV SM.</p></list-item><list-item><p><bold>Funding acquisition:</bold> RHS SRW VT.</p></list-item><list-item><p><bold>Investigation:</bold> EK CGC JS YL HHR RZ MHw HS PV RE SM MHa LCB NK CG.</p></list-item><list-item><p><bold>Project administration:</bold> VT.</p></list-item><list-item><p><bold>Resources:</bold> RHS MK BL CCB FJMvK JZ SRW UK VT.</p></list-item><list-item><p><bold>Supervision:</bold> RHS MK BL CCB FJMvK JZ SRW UK VT.</p></list-item><list-item><p><bold>Visualization:</bold> EK VT.</p></list-item><list-item><p><bold>Writing – original draft:</bold> EK VT.</p></list-item><list-item><p><bold>Writing – review & editing:</bold> EK CGC JS YL JW RZ MHw MHa LCB RHS MK BL CCB JZ SRW UK VT.</p></list-item></list></p></fn><corresp id="cor001">* E-mail: <email>Volker.thiel@vetsuisse.unibe.ch</email></corresp></author-notes><pub-date pub-type="epub"><day>3</day><month>2</month><year>2017</year></pub-date><pub-date pub-type="collection"><month>2</month><year>2017</year></pub-date><volume>13</volume><issue>2</issue><elocation-id>e1006195</elocation-id><history><date date-type="received"><day>29</day><month>7</month><year>2016</year></date><date date-type="accepted"><day>20</day><month>1</month><year>2017</year></date></history><permissions><copyright-statement>© 2017 Kindler et al</copyright-statement><copyright-year>2017</copyright-year><copyright-holder>Kindler et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="ppat.1006195.pdf"></self-uri><abstract><p>Coronaviruses are of veterinary and medical importance and include highly pathogenic zoonotic viruses, such as SARS-CoV and MERS-CoV. They are known to efficiently evade early innate immune responses, manifesting in almost negligible expression of type-I interferons (IFN-I). This evasion strategy suggests an evolutionary conserved viral function that has evolved to prevent RNA-based sensing of infection in vertebrate hosts. Here we show that the coronavirus endonuclease (EndoU) activity is key to prevent early induction of double-stranded RNA (dsRNA) host cell responses. Replication of EndoU-deficient coronaviruses is greatly attenuated <italic>in vivo</italic> and severely restricted in primary cells even during the early phase of the infection. In macrophages we found immediate induction of IFN-I expression and RNase L-mediated breakdown of ribosomal RNA. Accordingly, EndoU-deficient viruses can retain replication only in cells that are deficient in IFN-I expression or sensing, and in cells lacking both RNase L and PKR. Collectively our results demonstrate that the coronavirus EndoU efficiently prevents simultaneous activation of host cell dsRNA sensors, such as Mda5, OAS and PKR. The localization of the EndoU activity at the site of viral RNA synthesis–within the replicase complex—suggests that coronaviruses have evolved a viral RNA decay pathway to evade early innate and intrinsic antiviral host cell responses.</p></abstract><abstract abstract-type="summary"><title>Author summary</title><p>Coronaviruses are long known to be particularly successful in evading host innate immune responses. This manifests in barely detectable interferon responses during the first hours of infection and greatly facilitates establishment of robust virus replication. This phenotype of early innate immune evasion is common to all coronaviruses and can have detrimental consequences particular for infections with highly pathogenic coronaviruses, such as SARS-CoV and MERS-CoV. We therefore hypothesized that there must be an evolutionary conserved viral function that has evolved to prevent sensing of coronavirus infection by infected host cells. Our study now describes this function, namely the highly conserved coronavirus endoribonuclease activity. We found that coronaviruses that lack this enzymatic activity are readily visible to infected host cells that can now mount a swift and potent host response restricting virus replication within hours. Our study provides a new paradigm of a first layer of RNA virus innate immune evasion during the early phase of infection, that takes place at the site of RNA synthesis, and is based on removal of dsRNA that would otherwise trigger the simultaneous activation of cytoplasmic host cell sensors.</p></abstract><funding-group><award-group id="award001"><funding-source><institution-wrap><institution-id institution-id-type="funder-id">http://dx.doi.org/10.13039/501100001711</institution-id><institution>Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung</institution></institution-wrap></funding-source><award-id>149784</award-id><principal-award-recipient><contrib-id authenticated="true" contrib-id-type="orcid">http://orcid.org/0000-0002-5783-0887</contrib-id><name><surname>Thiel</surname><given-names>Volker</given-names></name></principal-award-recipient></award-group><award-group id="award002"><funding-source><institution-wrap><institution-id institution-id-type="funder-id">http://dx.doi.org/10.13039/100000002</institution-id><institution>National Institutes of Health</institution></institution-wrap></funding-source><award-id>R01-AI104887</award-id><principal-award-recipient><contrib-id authenticated="true" contrib-id-type="orcid">http://orcid.org/0000-0002-8155-4528</contrib-id><name><surname>Weiss</surname><given-names>Susan R.</given-names></name></principal-award-recipient></award-group><award-group id="award003"><funding-source><institution-wrap><institution-id institution-id-type="funder-id">http://dx.doi.org/10.13039/100000002</institution-id><institution>National Institutes of Health</institution></institution-wrap></funding-source><award-id>R01-AI104887</award-id><principal-award-recipient><name><surname>Silverman</surname><given-names>Robert H.</given-names></name></principal-award-recipient></award-group><award-group id="award004"><funding-source><institution-wrap><institution-id institution-id-type="funder-id">http://dx.doi.org/10.13039/100000002</institution-id><institution>National Institutes of Health</institution></institution-wrap></funding-source><award-id>R01-NS081008</award-id><principal-award-recipient><contrib-id authenticated="true" contrib-id-type="orcid">http://orcid.org/0000-0002-8155-4528</contrib-id><name><surname>Weiss</surname><given-names>Susan R.</given-names></name></principal-award-recipient></award-group><funding-statement>This work was supported by the Swiss National Science Foundation (SNF project 149784), and by NIH Grants R01-AI104887 (to SRW and RHS), R01-NS081008 (to SRW), and R01-CA044059 (to RHS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><fig-count count="8"></fig-count><table-count count="0"></table-count><page-count count="26"></page-count></counts><custom-meta-group><custom-meta><meta-name>PLOS Publication Stage</meta-name><meta-value>vor-update-to-uncorrected-proof</meta-value></custom-meta><custom-meta><meta-name>Publication Update</meta-name><meta-value>2017-02-15</meta-value></custom-meta><custom-meta id="data-availability"><meta-name>Data availability</meta-name><meta-value>All relevant data are within the paper and its Supporting Information files.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data availability</title><p>All relevant data are within the paper and its Supporting Information files.</p></notes></front><body><sec sec-type="intro" id="sec001"><title>Introduction</title><p>Host innate immune responses are of particular importance during the early phase of virus infection to restrict virus replication and spread. They rely on the ability to differentiate between immunological “self” and “non-self” in order to swiftly activate diverse antiviral effector mechanisms. Conceptually, sensing of virus infection is mainly mediated through recognition of viral nucleic acids, which are considered to comprise pathogen-associated molecular patterns (PAMPs) that are recognized by specialized host cell pathogen recognition receptors (PRRs) [<xref rid="ppat.1006195.ref001" ref-type="bibr">1</xref>]. Double-stranded (ds) RNA, an obligate replication intermediate of positive-stranded RNA viruses that is accumulating during replication, is known as an important PAMP within the cytoplasm of infected cells. Host cell responses to dsRNA are versatile and include the expression of IFN-I by activating RIG-I like helicases (RLRs), such as Rig-I and Mda5, the inhibition of host cell translation by activating PKR, and the degradation of viral and host cell-derived RNA by activating the OAS/RNase L pathway [<xref rid="ppat.1006195.ref002" ref-type="bibr">2</xref>].</p><p>Coronaviruses are positive-stranded RNA viruses that replicate in the host cell cytoplasm. They are well known to evade innate immune activation, particularly during the early phase of the infection [<xref rid="ppat.1006195.ref003" ref-type="bibr">3</xref>–<xref rid="ppat.1006195.ref006" ref-type="bibr">6</xref>]. Coronavirus innate immune evasion is multifaceted and involves ribose-2’-O methylation of viral RNA, as well as compartmentalised RNA synthesis at virus-induced membrane structures comprised of convoluted membranes and double membrane vesicles [<xref rid="ppat.1006195.ref007" ref-type="bibr">7</xref>–<xref rid="ppat.1006195.ref009" ref-type="bibr">9</xref>]. The importance of functions encoded by the CoV replicase gene is further exemplified by non-structural protein (nsp) 1 that suppresses host gene expression by mediating host mRNA degradation [<xref rid="ppat.1006195.ref010" ref-type="bibr">10</xref>, <xref rid="ppat.1006195.ref011" ref-type="bibr">11</xref>], and nsp3 that contains a papain-like proteinase with deubiquitination activity interfering with IFN-I host cell responses [<xref rid="ppat.1006195.ref012" ref-type="bibr">12</xref>, <xref rid="ppat.1006195.ref013" ref-type="bibr">13</xref>]. In addition, a number of accessory gene functions, although less conserved, have been described to target downstream events of innate immune activation, such as a phosphodiesterase (PDE) activity encoded by some coronavirus strains, which degrades 2’,5’-oligoadenylate messenger molecules essential for RNase L activation [<xref rid="ppat.1006195.ref014" ref-type="bibr">14</xref>, <xref rid="ppat.1006195.ref015" ref-type="bibr">15</xref>].</p><p>Here we addressed a possible role of the highly conserved coronavirus EndoU activity in innate immune evasion. The EndoU domain is harboured in non-structural protein (nsp) 15 that is considered as an integral component of the coronaviral replicase-transcriptase complex (RTC)[<xref rid="ppat.1006195.ref016" ref-type="bibr">16</xref>–<xref rid="ppat.1006195.ref019" ref-type="bibr">19</xref>]. By using immunofluorescence microscopy analyses in HCoV-229E-infected cells with a HCoV-229E-nsp15-specific monoclonal antibody the characteristic perinuclear staining pattern known from various other CoV nsps was reported [<xref rid="ppat.1006195.ref019" ref-type="bibr">19</xref>]. For MHV-A59, a similar study reports MHV-nsp15-specific perinuclear puncta that were detected using an MHV-nsp15-specific rabbit antiserum that partially overlapped with MHV nucleocapsid staining in MHV-A59-infected cells [<xref rid="ppat.1006195.ref020" ref-type="bibr">20</xref>, <xref rid="ppat.1006195.ref021" ref-type="bibr">21</xref>]. Moreover, MHV nsp15 was shown to co-localize with viral RNA and to fractionate in similar fractions as other nsps following MHV infection [<xref rid="ppat.1006195.ref021" ref-type="bibr">21</xref>, <xref rid="ppat.1006195.ref022" ref-type="bibr">22</xref>]. Notably, upon ectopic expression of a fusion protein comprised of the green fluorescent protein (GFP) and MHV-nsp15, a pattern of cytoplasmic speckles, distinct from the characteristic pattern of the CoV replicase complex was observed, suggesting that the localization of ectopically expressed nsp15 or GFP-nsp15 fusion proteins may differ from the localization of nsp15 that is expressed in the context of the CoV polyprotein 1ab [<xref rid="ppat.1006195.ref020" ref-type="bibr">20</xref>]. The CoV EndoU has uridylate-specific endonucleolytic activity on single-stranded and dsRNA[<xref rid="ppat.1006195.ref017" ref-type="bibr">17</xref>] and is related to (i) cellular enzymes prototyped by XendoU[<xref rid="ppat.1006195.ref016" ref-type="bibr">16</xref>, <xref rid="ppat.1006195.ref023" ref-type="bibr">23</xref>] and (ii) viral homologs conserved in all nidoviruses known to infect vertebrate hosts including fish, birds and mammals, suggesting an important role for this enzyme in an ancient cellular pathway. Over the past years, a wealth of structural and biochemical information has been obtained for EndoU. However, the precise role of this virus-encoded nucleolytic activity in coronavirus/nidovirus replication remains enigmatic [<xref rid="ppat.1006195.ref017" ref-type="bibr">17</xref>, <xref rid="ppat.1006195.ref018" ref-type="bibr">18</xref>, <xref rid="ppat.1006195.ref024" ref-type="bibr">24</xref>–<xref rid="ppat.1006195.ref026" ref-type="bibr">26</xref>]. Surprisingly, although EndoU is coexpressed with other key replicative proteins as part of the viral replicase polyprotein, its enzymatic activity is not essential for viral RNA synthesis in most cell culture systems[<xref rid="ppat.1006195.ref026" ref-type="bibr">26</xref>]. In this work we illustrate a pronounced impact of the coronavirus EndoU activity on innate immune evasion. Specifically, we show that genetically engineered mutants of mouse hepatitis virus (MHV) and human coronavirus 229E (HCoV-229E), respectively, that encode an EndoU active-site substitution known to abolish nucleolytic activity, were severely attenuated and elicited an immediate and simultaneous activation of host cell dsRNA sensors.</p></sec><sec sec-type="results" id="sec002"><title>Results</title><sec id="sec003"><title>Severe attenuation of EndoU-deficient coronaviruses</title><p>Based on biochemical and structural information on coronavirus EndoU active-site residues[<xref rid="ppat.1006195.ref017" ref-type="bibr">17</xref>, <xref rid="ppat.1006195.ref027" ref-type="bibr">27</xref>, <xref rid="ppat.1006195.ref028" ref-type="bibr">28</xref>], we constructed EndoU-deficient mutants of HCoV-229E and MHV (HCoV-229E<sub>H250A</sub> and MHV<sub>H277A</sub>) and assessed their replication characteristics <italic>in vitro</italic> and <italic>in vivo</italic> (<xref ref-type="fig" rid="ppat.1006195.g001">Fig 1A</xref>). Replication of MHV<sub>H277A</sub> was reduced in L929 cells, but peak titers almost reached those of wild-type MHV-A59, confirming that the coronavirus EndoU activity is dispensable for virus replication <italic>in vitro</italic> (<xref ref-type="fig" rid="ppat.1006195.g001">Fig 1B</xref>)[<xref rid="ppat.1006195.ref025" ref-type="bibr">25</xref>, <xref rid="ppat.1006195.ref026" ref-type="bibr">26</xref>]. In sharp contrast, compared to wild-type MHV-A59, the EndoU-deficient MHV<sub>H277A</sub> was severely attenuated <italic>in vivo</italic> (<xref ref-type="fig" rid="ppat.1006195.g001">Fig 1C</xref>). MHV<sub>H277A</sub> replication was not detectable in spleen and liver of C57BL/6 mice at two days post intraperitoneal infection with 500 plaque-forming units (pfu), demonstrating that the EndoU activity is required for efficient replication and spread <italic>in vivo</italic>. Notably, replication and spread of MHV<sub>H277A</sub> was partly restored in mice deficient for the IFN-I receptor (IFNAR), with viral titers of MHV<sub>H277A</sub> in the spleen and liver of IFNAR-deficient mice that did not reach those of MHV-A59. Interestingly, concerning the role of Mda5 and TLR7, which are known as main cytoplasmic and endosomal PRRs for coronaviral RNA, respectively, MHV<sub>H277A</sub> replication was not restored in Mda5-deficient, TLR7-deficient, or Mda5- and TLR7-deficient mice. This phenotype clearly differs from that described for coronaviruses that lack ribose-2’-O methyl-transferase (OMT) activity [<xref rid="ppat.1006195.ref009" ref-type="bibr">9</xref>]. Thus, in experiments reported previously, we found that replication of OMT-deficient MHV (MHV<sub>D130A</sub>) is restored in mice that are deficient for Mda5 and TLR7, suggesting that lack of ribose-2’-O methylation is tolerated if these two RNA sensors are absent. The lack of any detectable replication of the EndoU-deficient MHV<sub>H277A</sub> in Mda5- and TLR7-deficient mice therefore indicates that Mda5- and TLR7-mediated IFN-I expression may not exclusively restrict MHV<sub>H277A</sub> replication and that other mechanisms contribute to the observed attenuation of MHV<sub>H277A</sub> replication.</p><fig id="ppat.1006195.g001" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.ppat.1006195.g001</object-id><label>Fig 1</label><caption><title>The CoV endoribonuclease is essential for replication and spread in vivo.</title><p>(<bold>a</bold>) Genome organization of the EndoU-deficient murine hepatitis virus (MHV) with an active site His to Ala substitution (MHV<sub>H277A</sub>) and a corresponding human coronavirus 229E mutant (HCoV-229E<sub>H250A</sub>) in the non-structural protein 15. (<bold>b</bold>) Replication kinetics of MHV-A59 and MHV<sub>H277A</sub> in murine L929 fibroblasts after infection at a MOI of 1 and 0.1, presented as viral titer in plaque forming units (pfu). Data represent two independent experiments, each performed in duplicates. Mean and SEM are depicted. The 95% confidence band is highlighted in grey. The differences in peak levels of viral titers were calculated by using the non-linear regression model described in Material and Methods (peak MHV-A59: 6.0, MHV<sub>H277A</sub>: 5.6, p = 0.024, left panel; peak MHV-A59: 6.6, MHV<sub>H277A</sub>: 6.0, p = 0.016, right panel) and significance is displayed as * p < 0.05. (<bold>c</bold>) Viral titers of MHV-A59 and MHV<sub>H277A</sub> in liver and spleen of C57BL/6, IFNAR<sup>-/-</sup>, Mda5<sup>-/-</sup>, TLR7<sup>-/-</sup>, and Mda5<sup>-/-</sup>/TLR7<sup>-/-</sup> mice at two days post intraperitoneal infection (500 pfu). Data represent three to four independent experiments, each based on two to three mice per strain and virus. Mean and SEM are depicted. Data points that show significant differences in a two-sided, unpaired Student’s t-test are displayed; * p < 0.05, ** < 0.01, *** < 0.001. ND, not detected.</p></caption><graphic xlink:href="ppat.1006195.g001"></graphic></fig></sec><sec id="sec004"><title>EndoU-deficiency results in increased IFN-β expression</title><p>The severe attenuation of MHV<sub>H277A</sub> growth <italic>in vivo</italic> prompted us to assess MHV<sub>H277A</sub> and HCoV-229E<sub>H250A</sub> replication in primary target cells. As shown in <xref ref-type="fig" rid="ppat.1006195.g002">Fig 2A</xref>, MHV<sub>H277A</sub> replication in primary murine embryonic fibroblasts (MEFs) was comparable to that of MHV-A59 until 9–12 hours post infection (h.p.i.), but was significantly restricted later during the infection. Moreover, replication of MHV<sub>H277A</sub> was even more severely reduced in bone marrow-derived murine macrophages, and accompanied by early induction of IFN-β expression (<xref ref-type="fig" rid="ppat.1006195.g002">Fig 2B and 2C</xref>). Notably, levels of IFN-β mRNA were only transiently (6 to 12 h.p.i.) elevated in MHV<sub>H277A</sub> compared to MHV-A59 infected macrophages, and declined along with viral titers and viral RNA during the late phase of infection. Likewise, replication of the EndoU-deficient HCoV-229E<sub>H250A</sub> was severely restricted in human blood-derived macrophages (<xref ref-type="fig" rid="ppat.1006195.g002">Fig 2D</xref>). We observed significantly elevated IFN-I expression in a panel of human macrophages derived from seven individual donors after infection with HCoV-229E<sub>H250A</sub> compared to wild-type HCoV-229E infection (<xref ref-type="fig" rid="ppat.1006195.g002">Fig 2D</xref>), consistent with reduced viral replication.</p><fig id="ppat.1006195.g002" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.ppat.1006195.g002</object-id><label>Fig 2</label><caption><title>EndoU-deficient coronaviruses are severely attenuated in primary macrophages and trigger an elevated IFN-I response.</title><p>(<bold>a</bold>) Replication kinetics of MHV-A59 and MHV<sub>H277A</sub> in C57BL/6 mouse embryonic fibroblasts (MEFs) after infection at a MOI of 1, presented as viral titer in pfu. Data represent four independent experiments, each performed in two to four replicas. The difference in peak levels of viral titers (peak MHV-A59: 5.9, peak MHV<sub>H277A</sub>: 4.8) was statistically significant (***, p<0.001). (<bold>b</bold>) Replication kinetics of MHV-A59 and MHV<sub>H277A</sub> (left panel; titers in pfu) and cell-associated viral RNA (right panel; qRT-PCR) following infection of C57BL/6 bone marrow-derived macrophages (MOI = 1). Data represent eight (left panel) or five (right panel) independent experiments, each performed in two to three replicas. The difference in peak levels of viral titers (left panel: peak MHV-A59: 5.3, MHV<sub>H277A</sub>: 3.3) and the difference in peak levels of RNA copies (right panel: peak MHV-A59: 9.5, MHV<sub>H277A</sub>: 8.5) were statistically significant (p = 0.002, p = 0.018, respectively). (<bold>c</bold>) Expression of IFN-β mRNA (left panel; qRT-PCR) and protein (right panel; ELISA) in C57BL/6 macrophages following infection of MHV-A59 and MHV<sub>H277A</sub> (MOI = 1). Expression of IFN-β mRNA was normalized to levels of the household genes GAPDH and Tbp and is displayed relative to mock as ΔΔC<sub>T</sub>. The IFN-β ELISA detection limit is indicated with a dashed line. Data represent seven (left panel) or eight (right panel) independent experiments, each performed in two to three replicas. The difference in peak levels of IFN-β mRNA (MHV-A59: 13.4, MHV<sub>H277A</sub>: 15.8) was statistically significant (p = 0.04). Significance of IFN-β protein at 9 h.p.i. was assessed by a two-sided, Wilcoxon matched-pairs test (p = 0.016). <bold>(a-c)</bold> Mean and SEM are depicted. The 95% confidence band is highlighted in grey. Statistically significant comparisons are displayed; * p < 0.05, ** < 0.01, *** < 0.001. (<bold>d</bold>) Titers (pfu) of HCoV-229E wild type and HCoV-229E<sub>H250A</sub> (left panel) and expression of IFN-I (right panel; IFN-I bioassay) in human blood-derived macrophages, 24 hours after infection (MOI = 1). Data represent six (left panel) or seven (right panel) independent experiments, each performed in three to four replicas. Significance was assessed by a two-sided, unpaired Student’s t-test (left panel, p<0.001) and a Wilcoxon matched-pairs test (right panel; p = 0.016). <bold>(c-d)</bold> Mean and SEM are depicted. Statistically significant comparisons are displayed; * p < 0.05, *** < 0.001.</p></caption><graphic xlink:href="ppat.1006195.g002"></graphic></fig><p>Next, we addressed if, and to what extent, the growth defects observed for EndoU-deficient coronaviruses correlate with the induction of IFN-β expression. Mda5 has been described as the main RNA sensor of coronavirus infection in murine macrophages [<xref rid="ppat.1006195.ref029" ref-type="bibr">29</xref>]. Compared to wild-type macrophages, IFN-β expression was reduced in Mda5-deficient macrophages following MHV<sub>H277A</sub> infection, as shown by qRT-PCR and IFN-β ELISA (<xref ref-type="fig" rid="ppat.1006195.g003">Fig 3A and 3D</xref>). Surprisingly, and again in contrast to the phenotype of the OMT-deficient MHV<sub>D130A</sub> [<xref rid="ppat.1006195.ref009" ref-type="bibr">9</xref>], MHV<sub>H277A</sub> replication was not restored in Mda5-deficient macrophages (<xref ref-type="fig" rid="ppat.1006195.g003">Fig 3A</xref>). Similarly, although IFN-β expression was likewise reduced in MAVS-deficient macrophages, MHV<sub>H277A</sub> replication was not restored in MAVS-deficient macrophages (<xref ref-type="fig" rid="ppat.1006195.g003">Fig 3B and 3D</xref>). Even in IRF3/IRF5/IRF7 (IRF3/5/7 <sup>-/-</sup>) triple-knockout macrophages, that display an almost negligible induction of IFN-β expression, MHV<sub>H277A</sub> replication was not fully restored (<xref ref-type="fig" rid="ppat.1006195.g003">Fig 3C and 3D</xref>). IFN-β protein assessed by IFN-β ELISA was below detection in all three macrophage genotypes (Mda5<sup>-/-</sup>, MAVS<sup>-/-</sup>, IRF3/5/7<sup>-/-</sup>; <xref ref-type="fig" rid="ppat.1006195.g003">Fig 3D</xref>). These results indicate that MHV<sub>H277A</sub> replication is either highly sensitive to already marginal amounts of IFN-I, or that other antiviral host cell responses may contribute to the attenuation of MHV<sub>H277A</sub>.</p><fig id="ppat.1006195.g003" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.ppat.1006195.g003</object-id><label>Fig 3</label><caption><title>Replication of MHV<sub>H277A</sub> in primary macrophages with deficiencies in the IFN-I induction pathway.</title><p>Kinetics of viral replication (left panels) and IFN-β mRNA (right panels) in bone marrow-derived macrophages deficient for Mda5 <bold>(a)</bold>, MAVS <bold>(b)</bold> and in macrophages triple knockout for IRF3, IRF5 and IRF7 <bold>(c)</bold> following infection with MHV-A59 and MHV<sub>H277A</sub> (MOI = 1). (<bold>d</bold>) IFN-β in the supernatant of infected macrophages was measured using an ELISA. All values were outside of the detection limit of 15.6 pg/ml (dashed line) and thus depicted as ND (not detected). (<bold>a-d</bold>) Data represent three independent experiments, each performed in two to three replicas. <bold>(a)</bold> The difference in peak levels of viral titers (MHV-A59: 4.9, MHV<sub>H277A</sub>: 3.0) was statistically significant (p<0.001), the difference in peak levels of IFN-β expression (MHV-A59: 9.2, MHV<sub>H277A</sub>: 7.8) was statistically not significant (p = 0.44). <bold>(b)</bold> The differences in peak levels of viral titers (MHV-A59: 5.5, MHV<sub>H277A</sub>: 3.9) and IFN-β expression (MHV-A59: 9.1, MHV<sub>H277A</sub>: 12.1) were statistically significant (p<0.001, p = 0.024, respectively). <bold>(c)</bold> The difference in peak levels of viral titers (MHV-A59: 5.5, MHV<sub>H277A</sub>: 4.9) was statistically significant (p = 0.002). The difference in peak levels of IFN-β expression (MHV-A59: 6.5, MHV<sub>H277A</sub>: 5.1) was statistically not significant (p = 0.368). Mean and SEM are depicted. The 95% confidence band is highlighted in grey. Statistically significant comparisons are displayed; * p < 0.05, ** < 0.01, *** < 0.001.</p></caption><graphic xlink:href="ppat.1006195.g003"></graphic></fig></sec><sec id="sec005"><title>EndoU-deficient coronaviruses display a pronounced sensitivity to IFN-I treatment</title><p>In order to address the sensitivity of EndoU-deficient coronaviruses to IFN-I, we first assessed MHV<sub>H277A</sub> replication in IFNAR-deficient macrophages. As shown in <xref ref-type="fig" rid="ppat.1006195.g004">Fig 4A</xref>, MHV<sub>H277A</sub> replication was partially restored to levels that almost reached those of wild-type MHV-A59 replication. Importantly, IFN-β expression was elevated in IFNAR-deficient macrophages that had been infected with MHV<sub>H277A</sub> compared to those infected with wild-type MHV-A59, demonstrating that increased expression of IFN-β can be uncoupled from attenuation of MHV<sub>H277A</sub> (<xref ref-type="fig" rid="ppat.1006195.g004">Fig 4B</xref>). We also noted that IFN-β expression was delayed in IFNAR-deficient macrophages following MHV<sub>H277A</sub> infection compared to wild-type macrophages. This observation is in agreement with previous reports that suggested a macrophage-specific autocrine IFN-β priming loop in wild-type macrophages enhances cytokine and chemokine expression following MHV infection [<xref rid="ppat.1006195.ref030" ref-type="bibr">30</xref>].</p><fig id="ppat.1006195.g004" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.ppat.1006195.g004</object-id><label>Fig 4</label><caption><title>Replication of EndoU-deficient MHV is partially restored in IFNAR<sup>-/-</sup> macrophages and EndoU mutants display a pronounced sensitivity to IFN-I treatment.</title><p>(<bold>a</bold>) Replication kinetics of MHV-A59 and MHV<sub>H277A</sub> (left panel; titers in pfu) and cell-associated viral RNA (right panel; qRT-PCR) following infection of IFNAR<sup>-/-</sup> bone marrow-derived macrophages (MOI = 1). Data represent four independent experiments, each performed in two to three replicas. Mean and SEM are depicted. The 95% confidence band is highlighted in grey. The differences in peak levels of viral titers (MHV-A59: 6.0, MHV<sub>H277A</sub>: 5.2) and RNA copies (MHV-A59: 9.7, MHV<sub>H277A</sub>: 9.3) were statistically significant (p<0.001, p = 0.032, respectively). (<bold>b</bold>) Expression of IFN-β mRNA (left panel; qRT-PCR) and protein (right panel; ELISA) in IFNAR<sup>-/-</sup> macrophages following infection of MHV-A59 and MHV<sub>H277A</sub> (MOI = 1). Data represent four (left panel) and three (right panel) independent experiments, each performed in two to three replicas. Median and the 1–99 percentiles are displayed. Dashed line depicts limit of detection (right panel). The difference in peak levels of IFN-β expression (MHV-A59: 9.4, MHV<sub>H277A</sub>: 13.8) was statistically significant (p = 0.002). Significance of IFN-β expression was assesses by a Wilcoxon matched-pairs test, * p < 0.05. ND, not detected. (<bold>c</bold>) Sensitivity of wild type and EndoU-deficient MHV (left panel) and HCoV-229E (right panel) viruses to IFN-I pre-treatment (4 h) in L929 cells (left panel) and MRC-5 cells (right panel) with various dosages of IFN-I (MOI = 1). Virus replication was measured at 24 h.p.i. by plaque assay (MHV) and at 48 h.p.i. by qRT-PCR (HCoV-229E), respectively. Data represent three independent experiments, each performed in two to three replicas. Data are displayed as differences to untreated controls and statistical comparisons between wild type and EndoU-deficient viruses were performed for each concentration. Mean and SEM are displayed. Data points that show significant differences in a two-sided, unpaired Student’s t-test are depicted. * p < 0.05, ** p < 0.01 and *** p < 0001.</p></caption><graphic xlink:href="ppat.1006195.g004"></graphic></fig><p>The severe attenuation of MHV<sub>H277A</sub> and HCoV-229E<sub>H250A</sub> replication in wild-type murine and human macrophages, respectively, precluded the use of primary macrophages to assess the sensitivity of EndoU-deficient coronaviruses to IFN-I pre-treatment. Therefore, we infected murine L929 cells and human MRC5 lung fibroblasts with MHV<sub>H277A</sub> and HCoV-229E<sub>H250A</sub>, respectively, and applied different dosages of IFN-I for 4 hours prior to infection. Compared to wild type MHV and HCoV-229E, respectively, both EndoU-deficient viruses indeed displayed a pronounced sensitivity to IFN-I pre-treatment (<xref ref-type="fig" rid="ppat.1006195.g004">Fig 4C</xref>). Remarkably, MHV<sub>H277A</sub> displayed a sensitivity to IFN-I treatment that is comparable to that of the highly IFN-I sensitive OMT-deficient mutant MHV<sub>D130A</sub> (<xref ref-type="supplementary-material" rid="ppat.1006195.s001">S1 Fig</xref>). However, compared to the OMT-deficient mutant MHV<sub>D130A</sub> the phenotype of the EndoU-deficient MHV<sub>H277A</sub> differs mainly in the lack of restoration of replication under conditions with strongly reduced IFN-I expression (e.g. in Mda5<sup>-/-</sup> macrophages; <xref ref-type="fig" rid="ppat.1006195.g003">Fig 3A</xref>), suggesting that other, most likely IFN-I inducible, antiviral effector mechanisms account for restriction of MHV<sub>H277A</sub> replication. Collectively, these results demonstrate that the coronavirus EndoU activity plays a pivotal role in innate immune evasion in the context of the IFN-I system.</p></sec><sec id="sec006"><title>EndoU-deficient coronaviruses induce activation of the OAS/RNase L pathway</title><p>As noted above, coronavirus EndoU-deficiency results in a pronounced sensitivity to IFN-I treatment that is comparable to that of the highly IFN-I sensitive OMT-deficient mutant MHV<sub>D130A</sub>. However, replication of MHV<sub>H277A</sub> was not restored in Mda5-deficient macrophages. This observation prompted us to consider that replication of EndoU-deficient coronaviruses may activate additional dsRNA-triggered antiviral pathways. We therefore assessed the integrity of ribosomal RNA (rRNA), a marker for the activation of the OAS/RNase L pathway [<xref rid="ppat.1006195.ref031" ref-type="bibr">31</xref>], during MHV<sub>H277A</sub> infection in primary murine macrophages. Indeed, the breakdown of rRNA in MHV<sub>H277A</sub> infected wild-type macrophages was readily detectable as early as 6–12 h.p.i., thus coinciding with the induction of IFN-β expression during the early phase of the infection (compare Figs <xref ref-type="fig" rid="ppat.1006195.g005">5A</xref> and <xref ref-type="fig" rid="ppat.1006195.g002">2C</xref>). This finding is highly surprising since MHV-A59 encodes a PDE activity that has been shown to degrade 2’,5’-oligoadenylate messenger molecules essential for RNase L activation [<xref rid="ppat.1006195.ref014" ref-type="bibr">14</xref>]. However, the PDE activity was apparently not sufficient to prevent RNase L activation in macrophages that had been infected with EndoU-deficient MHV<sub>H277A</sub>. To exclude that the lack of EndoU activity may directly impact on viral RNA synthesis and lead to reduced levels of subgenomic mRNAs, we assessed the level of genomic and subgenomic mRNA2 (encoding the PDE activity) by qRT-PCR. As shown in <xref ref-type="supplementary-material" rid="ppat.1006195.s002">S2 Fig</xref>, genomic RNA and subgenomic mRNA2 were equally reduced in MHV<sub>H277A</sub> infected wild-type macrophages, suggesting that the lack of EndoU activity does not result in selective reduction of subgenomic mRNAs. Importantly, while breakdown of rRNA was also readily detectable in Mda5- and MAVS-deficient macrophages, rRNA remained intact in RNase L-deficient macrophages, demonstrating that infection of EndoU-deficient MHV<sub>H277A</sub> indeed results in the activation of the OAS-RNase L pathway and subsequent degradation of rRNA (<xref ref-type="fig" rid="ppat.1006195.g005">Fig 5A</xref>; <xref ref-type="supplementary-material" rid="ppat.1006195.s002">S2 Fig</xref>).</p><fig id="ppat.1006195.g005" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.ppat.1006195.g005</object-id><label>Fig 5</label><caption><title>EndoU-deficient MHV induces activation of the OAS-RNase L pathway, resulting in early breakdown of ribosomal RNA.</title><p>(<bold>a</bold>) Analysis of rRNA integrity in bone marrow-derived macrophages derived from wild type C57BL/6, Mda5<sup>-/-</sup>, RNase L<sup>-/-</sup>, and IFNAR<sup>-/-</sup> mice following infection with MHV-A59 and MHV<sub>H277A</sub> (MOI = 1). Total RNA was isolated at indicated time points and degradation of ribosomal RNA as marker for RNase L activation was assessed with a Fragment Analyzer. One representative picture and migration of 18S and 28S ribosomal RNA is displayed. The RNA Quality Number (RQN) is indicated. (<bold>b</bold>) The integrity of rRNA from MHV-A59 and MHV<sub>H277A</sub> infected (MOI = 1) L929 cells, with or without IFN-I pre-treatment (12.5 U of IFN-I 16h prior to infection). Analysis was performed as in panel (<bold>a</bold>) and one representative image out of five is displayed.</p></caption><graphic xlink:href="ppat.1006195.g005"></graphic></fig><p>Notably, a breakdown of rRNA was not detected in MHV<sub>H277A</sub>–infected IFNAR-deficient macrophages (<xref ref-type="fig" rid="ppat.1006195.g005">Fig 5A</xref>) concurring with partial restoration of MHV<sub>H277A</sub> replication in these cells. Accordingly, and as previously published [<xref rid="ppat.1006195.ref032" ref-type="bibr">32</xref>, <xref rid="ppat.1006195.ref033" ref-type="bibr">33</xref>], the degree of RNase L activation correlates with levels of OAS expression and we noted indeed reduced baseline expression of OAS 1a, 2 and 3 in IFNAR-deficient compared to wild-type C57BL/6 macrophages (<xref ref-type="supplementary-material" rid="ppat.1006195.s003">S3 Fig</xref>). Likewise, we did not observe breakdown of rRNA in L929 cells (<xref ref-type="fig" rid="ppat.1006195.g005">Fig 5B</xref>). We therefore assessed the levels of OAS 1a, 2, and 3 expression in L929 with or without IFN-I treatment (12.5 U). As expected, expression of IFN-β was elevated in MHV<sub>H277A</sub>–, but not in MHV-A59-infected L929 cells, irrespectively of IFN-I pre-treatment (<xref ref-type="supplementary-material" rid="ppat.1006195.s004">S4 Fig</xref>). Importantly however, expression of OAS 1a, 2 and 3 in L929 cells was significantly elevated following IFN-I treatment (<xref ref-type="supplementary-material" rid="ppat.1006195.s004">S4 Fig</xref>), and accordingly, rRNA breakdown was readily detectable in IFN-I treated L929 cells that had been infected with MHV<sub>H277A</sub> (<xref ref-type="fig" rid="ppat.1006195.g005">Fig 5B</xref>). This data provide evidence for a functional link between the observed pronounced IFN-I sensitivity of MHV<sub>H277A</sub> and restriction of MHV<sub>H277A</sub> replication by the OAS/RNase L pathway.</p></sec><sec id="sec007"><title>Involvement of PKR during early replication</title><p>Surprisingly however, MHV<sub>H277A</sub> replication was not restored in RNase L-deficient macrophages despite the fact that rRNA remained intact during the entire replication cycle (compare <xref ref-type="fig" rid="ppat.1006195.g005">Fig 5A</xref> and <xref ref-type="fig" rid="ppat.1006195.g006">Fig 6A</xref>). This strongly suggests that yet another antiviral pathway, in addition to OAS/RNase L, is activated during MHV<sub>H277A</sub> infection. One obvious candidate is PKR, a kinase that can be directly activated by dsRNA to phosphorylate the eukaryotic initiation factor 2α (eIF2α), resulting in translation inhibition of cellular and viral mRNAs. Indeed, as shown in <xref ref-type="fig" rid="ppat.1006195.g006">Fig 6B</xref> (left panel), we readily detected phosphorylated eIF2α at 9 h.p.i.. In addition, we assessed the extent of translational inhibition at 9 h.p.i. by using puromycin and subsequent FACS analysis. As shown in <xref ref-type="fig" rid="ppat.1006195.g006">Fig 6B</xref>, wild-type MHV-A59 infected cells showed active translation comparable to mock infected macrophages, while translational inhibition was observed in MHV<sub>H277A</sub>–infected macrophages. Finally, we assessed replication of MHV-A59 and MHV<sub>H277A</sub> in PKR-deficient macrophages, and as shown in <xref ref-type="fig" rid="ppat.1006195.g006">Fig 6C</xref>, PKR-deficiency alone was also not sufficient for the restoration of MHV<sub>H277A</sub> replication. Importantly, however, we observed elevated replication of MHV<sub>H277A</sub> in primary macrophages that are deficient for both, PKR and RNase L that almost reached that of MHV-A59 (<xref ref-type="fig" rid="ppat.1006195.g006">Fig 6D</xref>) [<xref rid="ppat.1006195.ref034" ref-type="bibr">34</xref>]. In order to more precisely analyse the degree of restoration of MHV<sub>H277A</sub> replication in IFNAR- and in RNase L/PKR-deficient macrophages, we performed a statistical analysis and compared the differences of calculated MHV-A59 and MHV<sub>H277A</sub> peak titers between C57BL/6 and IFNAR<sup>-/-</sup> (p = 0.008), between C57BL/6 and RNase L<sup>-/-</sup>/PKR<sup>-/-</sup> (p = 0.004) and between IFNAR<sup>-/-</sup> and RNase L<sup>-/-</sup>/PKR<sup>-/-</sup> (p = 0.612) (<xref ref-type="fig" rid="ppat.1006195.g006">Fig 6E</xref>). This result shows that MHV<sub>H277A</sub> replication is restored to a comparable degree in IFNAR<sup>-/-</sup> and RNase L<sup>-/-</sup>/PKR<sup>-/-</sup> macrophages.</p><fig id="ppat.1006195.g006" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.ppat.1006195.g006</object-id><label>Fig 6</label><caption><title>Involvement of RNase L and PKR in restricting replication of EndoU-deficient MHV.</title><p><bold>(a)</bold> Replication kinetics of MHV-A59 and MHV<sub>H277A</sub> following infection (MOI = 1) of bone marrow-derived RNase L<sup>-/-</sup> macrophages. Mean and SEM are shown. The 95% confidence bands are highlighted in grey. Data represent four independent experiments, each performed in two to three replicas. The difference in peak levels of viral titers (MHV-A59: 5.5, MHV<sub>H277A</sub>: 3.5) was statistically significant (***, p<0.001). <bold>(b)</bold> Intracellular staining of p-eif2α (left panel) and puromycin (right panel) and FACS analysis of MHV-A59 and MHV<sub>H277A</sub> infected (MOI = 1) C57BL/6 macrophages. One representative histogram out of three is shown. Phosphorylation of eif2α was determined using an antibody directed against p-eif2α. Cells without virus infection (mock) were used as controls (left panel). To label active translation (right panel), puromycin was added to the cells 15min prior to harvesting. Cells without puromycin-treatment (no puromycin) as well as cells without virus infection (mock) were used as controls. <bold>(c, d)</bold> Replication kinetics of MHV-A59 and MHV<sub>H277A</sub> following infection (MOI = 1) of bone marrow-derived PKR<sup>-/-</sup><bold>(c)</bold> and RNase L<sup>-/-</sup>/PKR<sup>-/-</sup><bold>(d)</bold> macrophages. Mean and SEM are shown. The 95% confidence bands are highlighted in grey. Data in <bold>(c)</bold> represent three independent experiments, each performed in two to three replicas. The difference in peak levels of viral titers (MHV-A59: 4.6, MHV<sub>H277A</sub>: 2.7) was statistically significant (**, p = 0.004). Data in <bold>(d)</bold> represent three independent experiments, each performed in two to three replicas. The difference in peak levels of viral titers (MHV-A59: 6.1, MHV<sub>H277A</sub>: 5.4) was statistically significant (*, p = 0.036). <bold>(e)</bold> Comparison of differences in peak titers calculated by using the non-linear regression model. Mean and 95% confidence intervals of calculated peak titers of MHV-A59 and MHV<sub>H277A</sub> following infection (MOI = 1) of bone marrow-derived C57BL/6 (data correspond to <xref ref-type="fig" rid="ppat.1006195.g002">Fig 2B</xref>), IFNAR<sup>-/-</sup> (data correspond to <xref ref-type="fig" rid="ppat.1006195.g004">Fig 4A</xref>) and RNase L<sup>-/-</sup>/PKR<sup>-/-</sup> (data correspond to <xref ref-type="fig" rid="ppat.1006195.g006">Fig 6C</xref>) macrophages are displayed. Statistical analysis was performed to compare differences of calculated MHV-A59 and MHV<sub>H277A</sub> peak titers between C57BL/6 and IFNAR<sup>-/-</sup> (**, p = 0.008), between C57BL/6 and RNase L<sup>-/-</sup>/PKR<sup>-/-</sup> (**, p = 0.004) and between IFNAR<sup>-/-</sup> and RNase L<sup>-/-</sup>/PKR<sup>-/-</sup> (p = 0.612; ns) macrophages following MHV-A59 and MHV<sub>H277A</sub> infection.</p></caption><graphic xlink:href="ppat.1006195.g006"></graphic></fig><p>Collectively, these results suggest that MHV<sub>H277A</sub> replication results in the early and simultaneous activation of at least three dsRNA-triggered pathways, namely IFN-β expression via Mda5, and antiviral effectors PKR and OAS/RNase L.</p></sec><sec id="sec008"><title>Infection with EndoU-deficient MHV results in increased cytosolic dsRNA</title><p>Since activation of Mda5, PKR and OAS/RNase L are triggered by dsRNA, we assessed if MHV<sub>H277A</sub> infection results in increased appearance of cytosolic dsRNA. By using the dsRNA-specific antibody J2 for intracellular staining and FACS analysis we assessed the level of dsRNA in MHV-A59- and MHV<sub>H277A</sub>-infected wild-type and IFNAR-deficient macrophages at 4, 6, 9, and 12 h.p.i.. At 4 h.p.i. dsRNA was not yet convincingly detectable in both MHV-A59- and MHV<sub>H277A</sub>-infected wild-type macrophages (<xref ref-type="fig" rid="ppat.1006195.g007">Fig 7A</xref>). At 6 h.p.i. dsRNA peaks are clearly separated over mock and we observed a slightly stronger dsRNA signal in MHV<sub>H277A</sub>- than in MHV-A59-infected cells. This difference became convincingly apparent and statistically significant at 9 and 12 h.p.i. with dsRNA peaks of MHV<sub>H277A</sub>-infected cells that clearly separated from dsRNA peaks of MHV-A59-infected cells (<xref ref-type="fig" rid="ppat.1006195.g007">Fig 7A and 7C</xref>, right panel). Importantly, we also controlled for virus infection by staining for the replicase complex (nsp2/3), and as shown in <xref ref-type="fig" rid="ppat.1006195.g007">Fig 7C</xref> (left panel) the peaks for nsp2/3 from in MHV<sub>H277A</sub>-infected wild-type macrophages did not exceed those of MHV-A59-infected cells. We obtained essentially the same result when we assessed dsRNA and nsp2/3 by FACS analysis following infection of IFNAR<sup>-/-</sup> macrophages (<xref ref-type="fig" rid="ppat.1006195.g007">Fig 7B and 7D</xref>), suggesting that dsRNA is also increased in MHV<sub>H277A</sub>-infection under conditions of reduced host cell responses. Collectively, these results demonstrate that cytosolic dsRNA is increased in EndoU-mutant virus infection and suggest that elevated dsRNA is the trigger for the activation of Mda5, PKR, and OAS.</p><fig id="ppat.1006195.g007" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.ppat.1006195.g007</object-id><label>Fig 7</label><caption><title>Infection with EndoU-deficient MHV results in increased cytosolic dsRNA.</title><p><bold>(a-b)</bold> Intracellular staining of dsRNA and FACS analysis of MHV-A59 and MHV<sub>H277A</sub> infected (MOI = 1) C57BL/6 <bold>(a)</bold> and IFNAR<sup>-/-</sup><bold>(b)</bold> macrophages at 4, 6, 9 and 12 h.p.i.. One representative histogram out of two (a) and three (b) is shown for each time point. Cells without virus infection (mock) were used as controls. <bold>(c-d)</bold> The left panels show cells that were co-stained for MHV-nsp2/3 to control for MHV-A59 and MHV<sub>H277A</sub> infection. The right panels display the median fluorescent intensity (MFI) of dsRNA peaks detected in <bold>(a-b).</bold> The left panels show data from two (c) and three (d) independent experiments. Cells without virus infection (mock) were used as controls. Mean and SEM are depicted. The 95% confidence band is highlighted in grey. Statistically significant comparisons are displayed (**, p< 0.01).</p></caption><graphic xlink:href="ppat.1006195.g007"></graphic></fig></sec></sec><sec sec-type="conclusions" id="sec009"><title>Discussion</title><p>Coronaviruses have long been known to efficiently evade host innate immune responses during the early phase of the infection. However, a defined viral function accounting for the apparent lack of efficient sensing of coronavirus infection has remained elusive. Here we show that the highly conserved coronavirus EndoU activity within the viral RTC plays a major role in providing a first line of innate immune evasion during the early phase of coronavirus infection.</p><p>We show that at least three dsRNA-triggered antiviral pathways are involved in restricting replication of EndoU-deficient coronaviruses (<xref ref-type="fig" rid="ppat.1006195.g008">Fig 8</xref>). First, infection with EndoU-deficient MHV and HCoV-229E results in rapid Mda5-mediated induction of IFN-β expression. Second, we observe breakdown of ribosomal RNA indicative of activation of the OAS/RNase L pathway that temporally coincides with IFN-β expression. Third, we show that efficient restriction of EndoU-deficient coronaviruses is furthermore dependent on PKR since restoration of EndoU-deficient MHV<sub>H277A</sub> replication required the absence of both, PKR and RNase L. Our data suggest that direct restriction of replication of EndoU-deficient coronaviruses is mediated by RNase L-mediated RNA degradation and inhibition of host cell translation through activation of PKR. In contrast, the effect of IFN-I appears to be indirect through the induction of ISG expression, that includes OAS/RNase L and PKR. Whether other ISGs may contribute to the restriction of EndoU-deficient coronavirus replication remains to be determined. Finally, we show that MHV<sub>H277A</sub> replication is associated with increased dsRNA levels during the early phase of the infection, providing a likely PAMP for the observed simultaneous activation of multiple cytoplasmic dsRNA-sensors in cells infected with EndoU-deficient coronaviruses.</p><fig id="ppat.1006195.g008" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.ppat.1006195.g008</object-id><label>Fig 8</label><caption><title>Coronavirus EndoU-mediated innate immune evasion.</title><p>Following coronavirus infection, the EndoU activity residing in the coronavirus replication complex prevents simultaneous activation of dsRNA sensors Mda5, OAS, and PKR. This strategy allows coronaviruses to efficiently evade antiviral innate host responses such as induction of IFN-I expression, RNase L-mediated RNA degradation, and inhibition of host cell translation.</p></caption><graphic xlink:href="ppat.1006195.g008"></graphic></fig><p>The concerted activation of multiple cytoplasmic antiviral pathways strongly suggests sensing of the same PAMP during replication of EndoU-deficient coronaviruses. All three types of sensors, MDA5, OAS1-3, and PKR, are known to recognize dsRNA, suggesting that the PAMP(s) relevant for their activation during infection is/are of viral origin. Notably, RNase L activation can be triggered by different OAS proteins and may depend on particular cell types and virus infections[<xref rid="ppat.1006195.ref035" ref-type="bibr">35</xref>]. For example overexpression of OAS 3 was shown to provide RNase L-dependent activity against dengue virus and chikungunya virus infection [<xref rid="ppat.1006195.ref036" ref-type="bibr">36</xref>, <xref rid="ppat.1006195.ref037" ref-type="bibr">37</xref>], while OAS 1 and OAS 3 have been implicated in antiviral activity against hepatitis C virus [<xref rid="ppat.1006195.ref038" ref-type="bibr">38</xref>]. It recently has been shown that among the human OAS proteins 1, 2, and 3, OAS 3 seems to be mainly responsible for mediating RNase L activation following either polyI:C transfection or virus infection, suggesting a superior role of human OAS 3 over OAS 1 and OAS 2 in restricting virus replication [<xref rid="ppat.1006195.ref033" ref-type="bibr">33</xref>]. Interestingly, structural and biochemical studies revealed that OAS 3 is selective for binding of long dsRNA (>50 bp) by involvement of an RNA-binding, but non-catalytic domain, and that OAS 3 is weakly or not activated by short dsRNA or single-stranded RNA, respectively [<xref rid="ppat.1006195.ref039" ref-type="bibr">39</xref>]. Likewise, PKR preferentially dimerizes upon binding to dsRNA of similar length (>60 bp) [<xref rid="ppat.1006195.ref040" ref-type="bibr">40</xref>, <xref rid="ppat.1006195.ref041" ref-type="bibr">41</xref>] and Mda5 is actually most efficiently activated by even longer dsRNA (>2 kbp) [<xref rid="ppat.1006195.ref042" ref-type="bibr">42</xref>] and higher-order structured RNA containing single-stranded and dsRNA [<xref rid="ppat.1006195.ref043" ref-type="bibr">43</xref>]. It is thus tempting to propose that viral dsRNA represents the natural substrate of the coronavirus EndoU. However, it remains to be determined which kind of viral dsRNA is cleaved by the EndoU or triggers Mda5, OAS, and PKR activation. Compared to MHV wild-type infection we observed a slight but reproducible increase of dsRNA in MHV<sub>H277A</sub>-infected macrophages by FACS analysis during the early phase of the infection (4–6 h.p.i.) that became more prominent at 9–12 h.p.i., suggesting that the majority of dsRNA is not cleaved by EndoU. This likely includes dsRNA being shielded within double-membrane vesicles and replication intermediates actively involved in viral RNA synthesis that are likely protected by the RTC and the nucleocapsid protein. We therefore speculate that coronaviruses may have evolved a viral RNA quality control mechanism to evade dsRNAs sensing, and that EndoU substrates may comprise dsRNA intermediates within stalled RTCs engaged in genome replication or transcription that are no longer active in viral RNA synthesis.</p><p>Within the order Nidovirales, the EndoU domain is highly conserved within the families <italic>Coronaviridae</italic> (comprising two subfamilies <italic>Coronavirinae</italic> and <italic>Torovirinae</italic>) and <italic>Arteriviridae</italic> and has been considered a major genetic marker that discriminates nidoviruses from all other RNA viruses [<xref rid="ppat.1006195.ref016" ref-type="bibr">16</xref>–<xref rid="ppat.1006195.ref018" ref-type="bibr">18</xref>]. However, the recent discovery of insect nidoviruses (family <italic>Mesoniviridae</italic>) [<xref rid="ppat.1006195.ref044" ref-type="bibr">44</xref>, <xref rid="ppat.1006195.ref045" ref-type="bibr">45</xref>] and re-analysis of the ronivirus genome (family <italic>Roniviridae</italic>; infecting crustaceans) revealed that these two nidovirus families do not encode an EndoU domain [<xref rid="ppat.1006195.ref044" ref-type="bibr">44</xref>]. In the light of our results it is tempting to speculate that the EndoU domain has evolved in vertebrate nidoviruses (<italic>Corona-</italic> and <italic>Arteriviridae</italic>) to counteract vertebrate-specific innate immune sensing of viral RNA in the context of the type-I interferon system, while the absence of the EndoU domain in roni- and mesoniviruses is indicative of fundamentally different mechanisms of RNA virus innate immune sensing and antiviral effector pathways in invertebrates (e.g. crustaceans and insects) [<xref rid="ppat.1006195.ref001" ref-type="bibr">1</xref>].</p><p>MHV as a natural mouse pathogen has been instrumental to understand the delicate balance between host IFN-I responses to infection and counteracting mechanisms of coronavirus innate immune evasion. While MHV evades innate immune sensing in most target cells, plasmacytoid dendritic cells (pDCs) remain as major IFN-I producer cells during early coronavirus replication to ensure protection of MHV target cells and control of potentially lethal coronavirus infections [<xref rid="ppat.1006195.ref046" ref-type="bibr">46</xref>, <xref rid="ppat.1006195.ref047" ref-type="bibr">47</xref>]. While pDCs sense coronaviral RNA within endosomes through TLR7, our data demonstrate that the coronaviral EndoU delays Mda5-mediated cytoplasmic sensing in macrophages and likely other cell types. This enables coronaviruses to establish robust replication and spread at the entry port of infection. However, in the case of highly pathogenic strains or newly emerging zoonotic coronaviruses, delayed IFN-I responses can have detrimental consequences as recently demonstrated in a murine model of SARS-CoV infection [<xref rid="ppat.1006195.ref003" ref-type="bibr">3</xref>]. Early and rapid SARS-CoV replication in the respiratory tract combined with a delayed IFN-I response can result in dysregulated innate immune responses and inflammatory cytokine-driven extensive lung damage. Therefore, antiviral intervention aiming at inhibiting the coronavirus EndoU activity may be a promising approach to restore efficient sensing of coronaviral RNA and thereby activating IFN-I expression as well as antiviral effector pathways such as PKR and OAS/RNase L.</p><p>There is a growing number of virus-encoded ribonucleases that have been reported to execute diverse steps in the context of cellular mRNA quality control and mRNA decay [<xref rid="ppat.1006195.ref048" ref-type="bibr">48</xref>, <xref rid="ppat.1006195.ref049" ref-type="bibr">49</xref>]. For example, herpesvirus-encoded endonucleases are known to broadly target cellular and also viral mRNAs that are subsequently further processed by cellular exonucleases, such as Xrn1, in order to broadly restrict host cell gene expression [<xref rid="ppat.1006195.ref050" ref-type="bibr">50</xref>]. While this strategy indirectly impacts host cell innate immune responses, a more specific interaction of RNA decay and host cell dsRNA responses has recently been described for vaccinia virus decapping enzymes D9 and D10 [<xref rid="ppat.1006195.ref051" ref-type="bibr">51</xref>, <xref rid="ppat.1006195.ref052" ref-type="bibr">52</xref>]. They remove 5’-cap structures of partially overlapping vaccinia virus mRNAs that arise during the late phase of infection in order to preclude any accumulation of viral dsRNA and subsequent activation of dsRNA sensors such as PKR and OAS. Notably, also in this case Xrn1 is required to further process the de-capped mRNAs. Our data show that early during coronavirus infection the EndoU activity conceptually fulfils the same task, namely the removal of dsRNA that would otherwise trigger host cell dsRNA responses, such as IFN-β expression and activation of PKR and OAS/RNase L. It will be interesting to address whether Xrn1 is involved in further degrading the coronavirus EndoU cleavage products or if this function could be fulfilled by the coronavirus-encoded exonuclease (ExoN) activity residing in nsp14. Interestingly, like the EndoU, the coronavirus ExoN is an integral component of the coronaviral RTC, and ExoN has been demonstrated to provide an RNA proofreading function that permits coronaviruses to stably maintain their extraordinary large RNA genome [<xref rid="ppat.1006195.ref053" ref-type="bibr">53</xref>–<xref rid="ppat.1006195.ref055" ref-type="bibr">55</xref>]. A functional link between the two coronaviral ribonucleases, ExoN and EndoU, would suggest an unprecedented concept of viral RNA quality/decay control that goes beyond RNA proofreading and includes the removal of dsRNA-based PAMPs at the site of RNA synthesis to efficiently evade innate and intrinsic antiviral host cell responses.</p></sec><sec sec-type="materials|methods" id="sec010"><title>Materials and methods</title><sec id="sec011"><title>Viruses</title><p>Recombinant MHV strain A59, HCoV-229E, MHV<sub>H277A</sub>, HCoV-229E<sub>H250A</sub>, and MHV<sub>D130A</sub> were generated using the vaccinia virus-based reverse genetic system as previously described [<xref rid="ppat.1006195.ref009" ref-type="bibr">9</xref>, <xref rid="ppat.1006195.ref056" ref-type="bibr">56</xref>–<xref rid="ppat.1006195.ref058" ref-type="bibr">58</xref>]. Viruses were propagated on 17Cl1 mouse fibroblasts (MHV) and on Huh-7 hepacarcinoma cells (HCoV-229E) and their identity was confirmed by sequencing.</p></sec><sec id="sec012"><title>Mice and viral infection</title><p>C57BL/6 mice were purchased from Charles River, from Jackson Laboratories and from the Department of Clinical Research, Bern, Switzerland (BE103/15). All genetically modified mice were produced on an inbred C57BL/6 background. Mice were bred and maintained at the Cantonal Hospital in St. Gallen (Mda5<sup>-/-</sup>, TLR7<sup>-/-</sup>, Mda5<sup>-/-</sup> / TLR7<sup>-/-</sup>, IFNAR<sup>-/-</sup>); at the Heimholtz Centre for Infection Research, Braunschweig or at the Twincore, Centre for Experimental and Infection Research, Hannover (MAVS<sup>-/-</sup>, IRF3/5/7<sup>-/-</sup>); Cleveland Clinic Lerner Research Institute, Cleveland (RNase L<sup>-/-</sup>, [<xref rid="ppat.1006195.ref059" ref-type="bibr">59</xref>]); at the Friedrich-Loeffler Institut, Greifswald (PKR<sup>-/-</sup>); at the Department of Cancer Biology, Lerner Research Institute, Cleveland (RNase L <sup>-/-</sup> / PKR<sup>-/-</sup>, [<xref rid="ppat.1006195.ref034" ref-type="bibr">34</xref>]). Mice were maintained in single ventilated cages in groups of maximally 5 individuals, were fed ad libitum and observed daily. All animal experiments were carried out in accordance with the animal legislation of the respective countries and their approval from the animal studies committees.</p><p>To monitor viral spread <italic>in vivo</italic>, mice at the age of 8–10 weeks were injected intraperitoneally with 500 plaque-forming units (pfu) of MHV-A59 and MHV<sub>H277A</sub>, diluted in MEM2% (2% heat-inactivated fetal calf serum, penicillin (60 μg/ml) and streptomycin (100 μg/ml)). Mice were euthanized two days post infection (d.p.i.) and liver and spleen were harvested, weighed and homogenized. Viral load (pfu/g organ) was determined by plaque assay.</p></sec><sec id="sec013"><title>Cells</title><p>Murine L929 fibroblasts (Sigma), 17Cl1 cells (gift from S.G. Sawicki) were cultured in MEM10%. Murine embryonic fibroblasts (C57BL/6 MEFs) were maintained at low passage in DMEM10% (Dulbecco’s Modified Eagle Medium-GlutaMAX). Huh-7 cells (gift from V. Lohnmann) were cultured in DMEM5% and 0.5mM sodium pyruvate. MRC-5 cells (human lung fibroblast-like cells; Sigma) were maintained at low passage in MEM10%, supplemented with 1% non-essential amino acids (NEAA). HEK293-Mx1-Luc cells (gift from G. Kochs) were maintained in DMEM10%, supplemented with G418 (200 μl/ml)[<xref rid="ppat.1006195.ref060" ref-type="bibr">60</xref>].</p></sec><sec id="sec014"><title>Isolation of murine and human primary macrophages</title><p>Murine bone marrow-derived macrophages were obtained from mice at the age of 8–12 weeks. Progenitor cells were isolated from hind limbs, passed through a cell strainer and red blood cell lysis was carried out in 1 ml lysis buffer/mouse (0.15 M NH<sub>4</sub>Cl, 1 mM KHCO<sub>3</sub>, 0.1 mM EDTA). Cells were washed 3x with PBS and taken up in macrophage medium (IMDM Iscove’s Modified Dulbecco’s Medium, 5–10% M-CSF (L929-supernatant), 0.1% 50 mM 2-mercaptoethanol). New medium was added 3 d.p.i. and adherent cells were harvested 7 d.p.i. Primary human macrophages were obtained from peripheral blood of healthy human donors as previously described[<xref rid="ppat.1006195.ref061" ref-type="bibr">61</xref>]. Peripheral blood mononuclear cells were isolated by centrifugation of buffy coat blood over a Leucosep tube (Greiner Bio One). Cells from the enriched interphase were collected, washed twice with PBS and red blood cells were removed. Cells were taken up in IMDM and plated in 24-well cell culture plates. Non-adherent cells were removed three h.p. seeding and adherent cells were cultured for 14 days in IMDM30%. Medium was changed every second day. All experiments using human blood were in accordance with the Swiss federal legislation and the institutional guidelines of the Cantonal Hospital St. Gallen and the Blutspendedienst SRK Bern.</p></sec><sec id="sec015"><title>Viral infections and measurement of viral burden</title><p>Murine cells were infected with MHV-A59 and MHV<sub>H277A</sub> (MOI = 0.1 L929 cells; or 1 MEFs, L929 cells, macrophages) at 37°C. Virus inoculum was removed 2 h.p.i., cells were washed with PBS and fresh medium was added. Virus supernatant was harvested and cellular RNA was collected in TRIzol (Invitrogen). Human macrophages (counted at the day of infection) and MRC5 cells were infected with HCoV-229E and HCoV-229E<sub>H250A</sub> (MOI = 1), virus inoculum was removed 2 h.p.i. (macrophages) and 4 h.p.i (MRC5), cells were washed and supernatant was harvested 24 h.p.i (macropahges) and 48 h.p.i (MRC5). Viral titer in the supernatant was determined by standard plaque titration on L929 cells (MHV) and Huh7 cells (HCoV-229E).</p></sec><sec id="sec016"><title>RNA isolation and quantitative RT-PCR</title><p>Total cellular RNA was isolated from murine macrophages with TRIzol (Life Technologies) and genomic DNA was removed with DNase (Ambion, DNA-free DNase Treatment). RNA concentration was measured by nanodrop and input for cDNA synthesis was standardized to 300 ng. Synthesis of cDNA was carried out using the M-MLV reverse transcriptase from Promega and the 20 μl cDNA were diluted with 80 μl dH<sub>2</sub>O. The FastStart Universal SYBR Green Master (Rox) Mix (Roche) was used for measuring mRNA expression of IFN-β, GAPDH and Tbp (<xref ref-type="supplementary-material" rid="ppat.1006195.s005">S1 Table</xref>). Induction of IFN-β was normalized to levels of the household genes GAPDH and Tbp (geometric mean) and expressed as ΔΔC<sub>T</sub> over mock (ΔC<sub>T</sub> values calculated as C<sub>T</sub> reference—C<sub>T</sub> target) [<xref rid="ppat.1006195.ref062" ref-type="bibr">62</xref>]. Expression of OAS1a, OAS2, OAS3 and RNase L mRNA in mock infected macrophages and L929 cells was normalized to levels of GAPDH [<xref rid="ppat.1006195.ref063" ref-type="bibr">63</xref>] (<xref ref-type="supplementary-material" rid="ppat.1006195.s005">S1 Table</xref>). Expression levels were displayed as ΔC<sub>T</sub> values (C<sub>T</sub> reference—C<sub>T</sub> target). Copy number of cell-associated viral RNA isolated from MHV infected macrophages was determined using the RT TaqMan PCR system (TaqMan Fast Universal PCR Master Mix (2x), No AmpErase UNG, Applied Biosystems) with primers and probe specific to the MHV genome fragment encoding the nucleocapsid (<xref ref-type="supplementary-material" rid="ppat.1006195.s005">S1 Table</xref>). Copy numbers were determined by using a standard curve, consisting of an <italic>in vitro</italic> transcribed RNA of known copy number, obtained from a plasmid comprising the MHV-nucleoprotein sequence.</p></sec><sec id="sec017"><title>Quantitative RT-PCR to determine MHV genomic RNA and subgenomic mRNA</title><p>Two RNA standards encompassing MHV nucleotides (nts) 15–530 (genomic standard), and MHV nts 15-63/21746-22259 (corresponding to the MHV mRNA2 leader-body junction; subgenomic mRNA2 standard) were prepared as follows. One RT-PCR product corresponding to the 5’ region of MHV-A59 genomic RNA was generated by RT-PCR using viral RNA from MHV-A59 infected cells as template and primers T7-MHV-leader15-frw and MHV-ORF1-rev530 (<xref ref-type="supplementary-material" rid="ppat.1006195.s006">S2 Table</xref>). The resulting RT-PCR product comprised the T7-RNA-Polymerase promoter, MHV nts 15–530. A second RT-PCR product corresponding to the 5’ region of MHV-A59 mRNA2 was generated by RT-PCR using viral RNA from MHV-A59 infected cells as template and primers T7-MHV-leader15-frw and MHV-ns2-rev22259 (<xref ref-type="supplementary-material" rid="ppat.1006195.s006">S2 Table</xref>). The resulting RT-PCR product comprised the T7-RNA-Polymerase promoter, MHV nts 15–63, and MHV nts 21746–22259. Both RT-PCR products were separated on an agarose gel and DNA fragments of the appropriate size were excised and purified. <italic>In vitro</italic> transcribed (IVT) RNA was prepared using the RiboMAX Large Scale RNA Production System–T7 (Promega). RQ1 RNase-free DNase was added to the IVT RNA and incubated for 15 minutes at 37°C. The <italic>in vitro</italic> transcribed RNA was purified using the NucleoSpin RNA kit (Macherey-Nagel), its quantity was determined (absorbance at 260nm) and eight 10-fold dilutions were prepared. Synthesis of cDNA was carried out for each dilution using the M-MLV reverse transcriptase and random primers (Promega). The 20 ul of cDNA were diluted with 80 μl dH<sub>2</sub>O and used as a standard for the quantitative RT-PCR reaction.</p><p>Copy numbers of genomic and subgenomic viral RNA were determined for samples obtained from C57BL/6 macrophages infected with MHV-A59 and and MHV<sub>H277A</sub> (MOI = 1). A multiplex reaction (TaqMan Fast Universal PCR Master Mix (2x), No AmpErase UNG, Applied Biosystems) and primers and a probe specific to the genomic or subgenomic sequence (<xref ref-type="supplementary-material" rid="ppat.1006195.s006">S2 Table</xref>), respectively, were used.</p></sec><sec id="sec018"><title>IFN-β Enzyme-Linked Immunosorbent Assay (ELISA), IFN-bioassay and IFN-pre-treatment</title><p>Total type-I IFN in supernatants obtained from MHV infected macrophages was measured by an IFN-β enzyme-linked immunosorbent assay (BPL Assay Science, VeriKine Mouse IFN Beta ELISA Kit, 15.6–1000 pg/ml). Technical replicates were pooled. The level of biologically active human type I IFN in the supernatant of infected human macrophages was measured with HEK293 cells that were stably transfected with a luciferase reporter plasmid under the control of the Mx-promoter [<xref rid="ppat.1006195.ref060" ref-type="bibr">60</xref>, <xref rid="ppat.1006195.ref064" ref-type="bibr">64</xref>]. Recombinant IFNα A/D (Sigma) was used as a cytokine standard and luciferase activity was detected 16 hours p.i. by a Luminometer (Luciferase Assay System, Promega). To assess the sensitivity of MHV and HCoV-229E towards IFN-I, L929 cells (MHV) and MRC5 cells (HCoV-229E) were treated with recombinant IFNα A/D (Sigma, as indicated in the figures) for four hours, and then infected with MHV-A59, MHV<sub>H277A</sub>, MHV<sub>D130A</sub>, HCoV-229E and HCoV<sub>H250A</sub> (MOI = 1). Virus supernatant was harvested 24 h.p.i (MHV) and 48 h.p.i (HCoV-229E). Viral replication was measured by plaque assay (MHV) or by using primers and a probe specific to the HCoV-229E membrane protein (<xref ref-type="supplementary-material" rid="ppat.1006195.s005">S1 Table</xref>) and the QuantiTect Probe OneStep RT-PCR Kit (Qiagen). To assess baseline expression, L929 cells were pre-treated with 12.5 U of IFNα A/D for 16h and then infected with MHV-A59 and MHV<sub>H277A</sub> (MOI = 1). Cellular RNA was isolated with TRIzol, cDNA was prepared and qRT-PCR was performed (<xref ref-type="supplementary-material" rid="ppat.1006195.s005">S1 Table</xref>).</p></sec><sec id="sec019"><title>Measurement of RNA quality with Fragment Analyzer</title><p>Total RNA isolated from MHV-infected macrophages and L929 cells was analysed with a Fragment Analyzer (Labgene) using the DNF-471 standard sensitivity RNA analysis kit (15nt lower marker, Advanced Analytical Technologies).</p></sec><sec id="sec020"><title>Flow Cytometry (FACS)</title><p>To assess the amount of dsRNA-positive cells, C57BL/6 and IFNAR<sup>-/-</sup> macrophages (5x10<sup>6</sup> cells) were infected with MHV-A59 and MHV<sub>H277A</sub> (MOI = 1). For FACS, cells were detached with PBS at 4°C, centrifuged and fixed with 4% formalin. Cells were permeabilized with 0.1% Triton and stained with the mouse monoclonal antibody J2 directed against dsRNA (1:200, English & Scientific Consulting Bt) and the anti-MHV nsp2/3 rabbit antiserum[<xref rid="ppat.1006195.ref065" ref-type="bibr">65</xref>] (1:600) at 4°C for 1h. Cells were washed, stained with a secondary antibody Goat F(ab’)2 anti-mouse IgG2a, human ads-PE (1:200, SouthernBiotech) and a donkey anti-rabbit Alexa-647 (1:400) for 30min. FACS was performed using the BD FACS Canto II and data were analysed using FlowJo v.10. Cell debris was excluded based on a gate of FSCA/SSCA, followed by a doublet discrimination FSCA and FSW. To assess the extent of translation inhibition, C57BL/6 macrophages were infected with MHV-A59 and MHV<sub>H277A</sub> (MOI = 1). 15 min prior to each time point, puromycin (Sigma) was added to the wells at a final concentration of 20μg/ml to label active translation. At the indicated time points, cells were washed twice with PBS, and subsequently detached with PBS at 4C. Cells were fixed in 2% PFA for 30min at RT, and washed once with FACS buffer (PBS + 1% BSA). Cells were incubated in ice-cold methanol for 10min at 4C. After two wash steps in FACS buffer, cells were incubated with a primary mouse antibody directed against puromycin (1:100, Milipore) and a primary rabbit anti-eIF2α-P (Abcam; 1:100) in FACS buffer for 45min at RT. Cells were washed twice with FACS buffer and incubated with the secondary antibody donkey anti-mouse-Alexa488 (1:200), and donkey anti-rabbit-Alexa648 (1:200) in FACS buffer for 45min at RT in the dark. Cells were washed once in FACS buffer, and kept in 1% PFA in the dark until cells were analysed with the FACS Canto (BD) using the BD FACS Diva software.</p></sec><sec id="sec021"><title>Statistical analysis of data</title><p>Kinetics of virus growth, viral RNA and IFN-β mRNA were analyzed using non-linear regression. The regression model is an exponential saturation model (increasing response with constant asymptote) that additionally allows a peak response. It is described by the formula
<disp-formula id="ppat.1006195.e001"><alternatives><graphic xlink:href="ppat.1006195.e001.jpg" id="ppat.1006195.e001g" mimetype="image" position="anchor" orientation="portrait"></graphic><mml:math id="M1"><mml:mrow><mml:mi>Y</mml:mi><mml:mrow><mml:mo>(</mml:mo><mml:mi>T</mml:mi><mml:mo>)</mml:mo></mml:mrow><mml:mo>=</mml:mo><mml:msub><mml:mi>A</mml:mi><mml:mi>G</mml:mi></mml:msub><mml:mo>−</mml:mo><mml:msup><mml:mi>e</mml:mi><mml:mrow><mml:mfrac><mml:mrow><mml:msub><mml:mi>M</mml:mi><mml:mi>G</mml:mi></mml:msub><mml:mo>−</mml:mo><mml:mi>T</mml:mi></mml:mrow><mml:mi>S</mml:mi></mml:mfrac></mml:mrow></mml:msup><mml:mo>+</mml:mo><mml:msub><mml:mi>P</mml:mi><mml:mi>G</mml:mi></mml:msub><mml:mo>⋅</mml:mo><mml:msup><mml:mi>e</mml:mi><mml:mrow><mml:mo>−</mml:mo><mml:msup><mml:mrow><mml:mrow><mml:mo>(</mml:mo><mml:mrow><mml:mfrac><mml:mrow><mml:msub><mml:mi>M</mml:mi><mml:mi>G</mml:mi></mml:msub><mml:mo>−</mml:mo><mml:mi>T</mml:mi></mml:mrow><mml:mi>S</mml:mi></mml:mfrac></mml:mrow><mml:mo>)</mml:mo></mml:mrow></mml:mrow><mml:mn>2</mml:mn></mml:msup></mml:mrow></mml:msup></mml:mrow></mml:math></alternatives></disp-formula>where <italic>Y</italic> is the response value (log virus titer or log expression value), <italic>T</italic> is the time (in hours), <italic>A</italic> is the value of the asymptote, <italic>M</italic> is a „midpoint value”representing the time where the exponential increase has reached half of the asymptotic value and where the peak is located, <italic>P</italic> is a value describing the additional peak height, and <italic>S</italic> is a scale parameter specifying the steepness of the exponential increase and the width of the peak. The coefficients <italic>A</italic>, <italic>M</italic> and <italic>P</italic> were determined individually for the groups to be compared (MHV-A59 and MHV<sub>H277A</sub>), as symbolized by the index <italic>G</italic>. The model was chosen on pragmatic grounds because it was able to describe the time courses of all data very well and the coefficients represent biologically relevant aspects of the kinetics that can be addressed directly by statistical tests. The analyses were performed in R, version 3.2.3 [<xref rid="ppat.1006195.ref066" ref-type="bibr">66</xref>] with the function nls [<xref rid="ppat.1006195.ref067" ref-type="bibr">67</xref>] using the algorithm “Port” restricting the coefficients to positive values. P-values and confidence intervals were determined by parametric bootstrapping, resampling the residuals from a normal distribution with mean 0 and variance estimated from the variance of residuals of the fitted model. Confidence bands were generated by connecting the point-wise 95% confidence intervals of the predictions. The significance of the difference between treatments in differences between maxima of the groups (i.e., the Group-Treatment interaction) was determined by bootstapping the difference-in-difference. The assumption of normally distributed residuals was checked and confirmed with normal-quantile quantile plots.</p><p>All other data were analysed using R v. 3.0.3 (R Development Core team, 2008) and displayed using GraphPad Prism v.5 and CorelDraw Graphic Suite X4. The types of statistical tests used are indicated in the corresponding figure legends.</p></sec><sec id="sec022"><title>Ethics statement</title><p>C57BL/6 mice, Mda5<sup>−/−</sup> mice, TLR7<sup>−/−</sup> mice, Mda5<sup>−/−</sup>/TLR7<sup>−/−</sup> mice, IFNAR<sup>−/−</sup> mice, were bred and maintained in the Kantonsspital St.Gallen animal facility and the University of Bern animal facility in accordance with federal and cantonal guidelines (Tierschutzgesetz). The protocols were approved by the Kantonal Veterinary Offices of the Kanton St.Gallen (SG09/87 and SG09/14 and SG10/14) and Kanton Bern (BE103/15).</p><p>C57BL/6 mice, and RNase L<sup>−/−</sup>, and RNase L<sup>−/−</sup>/PKR<sup>−/−</sup> mice were bred and maintained in the University of Pennsylvania and the Cleveland Clinic animal facilities. Protocols were in accordance with the National Institutes of Health (United States) Public Health Service (PHS) Policy on Humane Care and Use of Laboratory Animals, revised 2015, and approved by the Federal Government Animal Welfare Assurance for the University of Pennsylvania (Reference Assurance # A3079-01) and Institutional Animal Care and Use Committee at the University of Pennsylvania and the Federal Government Animal Welfare Assurance for <italic>the</italic> Cleveland Clinic (Reference Assurance # A3145-01).</p><p>C57BL/6 mice, MAVS<sup>-/-</sup>, and mice, IRF3/5/7<sup>-/-</sup> mice were bred and maintained in the animal facility of the TWINCORE, Hannover, Germany, and the protocols were approved by the Niedersächsisches Landesamt für Verbraucherschutz und Lebensmittelsicherheit.</p><p>PKR<sup>−/−</sup> mice were bred and maintained in the animal facility of the Friedrich-Loeffler-Institute, Insel Riems, and the protocols were approved by the Landesamt für Landwirtschaft, Lebensmittelsicherheit und Fischerei (LALLF) Mecklenburg-Vorpommern (LALLF 7221.3–2.1-011/13.).</p><p>All experiments using human blood (buffy coat) were in accordance with the Swiss federal legislation and the institutional guidelines (including informed consent) of the Cantonal Hospital St. Gallen and the Blutspendedienst SRK Bern.</p></sec></sec><sec sec-type="supplementary-material" id="sec023"><title>Supporting information</title><supplementary-material content-type="local-data" id="ppat.1006195.s001"><label>S1 Fig</label><caption><title>The sensitivity of MHV<sub>H277A</sub> towards IFN-I is in the range observed for the highly sensitive 2’O-methyltransferase mutant.</title><p>Comparative analysis of the sensitivity of MHV-A59, MHV<sub>H277A</sub> and MHV<sub>D130A</sub> (lacking the 2’-O methyltransferase activity) to IFN-I pre-treatment. L929 cells were pre-treated with various dosages of type-I IFN for four hours. Thereafter, cells were infected at an MOI of 1 and virus titers in the supernatant were measured at 24 h.p.i. by plaque assay. Data represent two independent experiments. Data from MHV-A59 and MHV<sub>H277A</sub> were already displayed in <xref ref-type="fig" rid="ppat.1006195.g004">Fig 4C</xref>. Data are displayed as differences to untreated controls. Mean and SEM are displayed.</p><p>(TIF)</p></caption><media xlink:href="ppat.1006195.s001.tif"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material><supplementary-material content-type="local-data" id="ppat.1006195.s002"><label>S2 Fig</label><caption><title>EndoU deficient mutant triggers rRNA breakdown in MAVS<sup>-/-</sup> and IRF3/5/7<sup>-/-</sup> macrophages.</title><p><bold>(a)</bold> Degradation of rRNA in MHV-A59 and MHV<sub>H277A</sub> infected (MOI = 1) MAVS<sup>-/-</sup> and IRF3/5/7<sup>-/-</sup> bone marrow-derived macrophages. Total RNA was isolated at indicated time points and degradation of ribosomal RNA was assessed with a Fragment Analyzer. One representative picture and migration of 18S and 28S ribosomal RNA is displayed. The RNA Quality Number (RQN) is indicated. <bold>(b)</bold> Cell-associated viral RNA following infection of C57BL/6 bone marrow-derived macrophages (MOI = 1) with MHV-A59 and MHV<sub>H277A</sub>. Copy numbers of genomic RNA (gRNA) and subgenomic RNA (mRNA2) are indicated. Data represent five independent experiments with mean and SEM displayed.</p><p>(TIF)</p></caption><media xlink:href="ppat.1006195.s002.tif"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material><supplementary-material content-type="local-data" id="ppat.1006195.s003"><label>S3 Fig</label><caption><title>Assessment of OAS1a, OAS2, OAS3 and RNase L mRNA baseline expression.</title><p>Baseline expression levels of OAS1a, OAS2, OAS3, RNase L mRNA in C57BL/6, Mda5<sup>-/-</sup>, MAVS<sup>-/-</sup>, RNase L<sup>-/-</sup>, PKR<sup>-/-</sup>, IRF3/5/7<sup>-/-</sup>, IFNAR<sup>-/-</sup>, and RNase L<sup>-/-</sup>/PKR<sup>-/-</sup>macrophages was assessed by qRT-PCR. Levels of mRNA expression relative to GAPDH are shown as log<sub>10</sub>(2<sup>ΔCT</sup>), where ΔC<sub>T</sub> = (C<sub>T</sub> reference—C<sub>T</sub> target). Bars in black highlight macrophage genotypes that restrict the replication of MHV<sub>H277A</sub>, while white bars highlight macrophage genotypes that at least partially permit MHV<sub>H277A</sub> replication. Statistical comparisons were carried out between mRNA expression levels measured in C57BL/6 cells versus knockout cells. Mean and SEM are displayed. Data points that show significance differences in a two-sided, unpaired Student’s t-test are depicted: * p < 0.05, ** p < 0.01, *** < 0.001. ND, not detected.</p><p>(TIF)</p></caption><media xlink:href="ppat.1006195.s003.tif"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material><supplementary-material content-type="local-data" id="ppat.1006195.s004"><label>S4 Fig</label><caption><title>EndoU-deficient MHV induces elevated expression of IFN-β and assessment of OAS1a-3 and RNase L mRNA expression levels in L929 cells.</title><p>(<bold>a</bold>) Replication kinetics of MHV-A59 and MHV<sub>H277A</sub> following infection (MOI = 1) of L929 cells, which were left untreated (left panel) or were pre-treated for 16h with 12.5 U of IFN-I (right panel). Data represent four independent experiments, each performed in duplicates. Mean and SEM are depicted. The 95% confidence bands are highlighted in grey. Statistically significant comparisons are displayed; *** p < 0.001. The differences in peak levels of IFN-β expression (left panel: MHV-A59: 6.2, MHV<sub>H277A</sub>: 13.6; right panel: MHV-A59: 7.9, MHV<sub>H277A</sub>: 15.9) were statistically significant (***; p = <0.001). (<bold>b</bold>) Analysis of baseline expression levels of OAS1a, OAS2, OAS3, and RNase L mRNA by qRT-PCR of L929 cells that were left untreated (black bars) or were pre-treated with 12.5 U of IFN-I for 16h (white bars). Levels of mRNA expression relative to GAPDH are shown as log<sub>10</sub>(2<sup>ΔCT</sup>), where ΔC<sub>T</sub> = (C<sub>T</sub> reference—C<sub>T</sub> target). Data represent four independent experiments, each performed in duplicates. Mean and SEM are displayed. Data points that show significant differences in a two-sided unpaired Student’s t-test are depicted: * p < 0.05, ** < 0.01.</p><p>(TIF)</p></caption><media xlink:href="ppat.1006195.s004.tif"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material><supplementary-material content-type="local-data" id="ppat.1006195.s005"><label>S1 Table</label><caption><title>Primers and probes used in qRT-PCR.</title><p>(PDF)</p></caption><media xlink:href="ppat.1006195.s005.pdf"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material><supplementary-material content-type="local-data" id="ppat.1006195.s006"><label>S2 Table</label><caption><p><bold>(A)</bold> Preparation of genomic and subgenomic RNA standards. <bold>(B)</bold> Primers and probes used in the multiplex qRT-PCR reaction.</p><p>(PDF)</p></caption><media xlink:href="ppat.1006195.s006.pdf"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material></sec></body><back><ack><p>We would like to thank Muriel Fragnière, Arnaud Baumann, and Ronald Dijkman for various technical assistance. We are grateful to Susan Baker for providing the anti-MHV nsp2/3 rabbit antiserum. 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