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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification and Expression of Equine MER-Derived miRNAs</title><author><name sortKey="Gim, Jeong An" sort="Gim, Jeong An" uniqKey="Gim J" first="Jeong-An" last="Gim">Jeong-An Gim</name><affiliation><nlm:aff id="af1-molce-40-4-262">Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 46241,<country>Korea</country></nlm:aff></affiliation><affiliation><nlm:aff id="af2-molce-40-4-262">Genetic Engineering Institute, Pusan National University, Busan 46241,<country>Korea</country></nlm:aff></affiliation><affiliation><nlm:aff id="af3-molce-40-4-262">The Genomics Institute, Life Sciences Department, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919,<country>Korea</country></nlm:aff></affiliation></author><author><name sortKey="Kim, Heui Soo" sort="Kim, Heui Soo" uniqKey="Kim H" first="Heui-Soo" last="Kim">Heui-Soo Kim</name><affiliation><nlm:aff id="af1-molce-40-4-262">Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 46241,<country>Korea</country></nlm:aff></affiliation><affiliation><nlm:aff id="af2-molce-40-4-262">Genetic Engineering Institute, Pusan National University, Busan 46241,<country>Korea</country></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28320202</idno><idno type="pmc">5424272</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5424272</idno><idno type="RBID">PMC:5424272</idno><idno type="doi">10.14348/molcells.2017.2295</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000F26</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000F26</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Identification and Expression of Equine MER-Derived miRNAs</title><author><name sortKey="Gim, Jeong An" sort="Gim, Jeong An" uniqKey="Gim J" first="Jeong-An" last="Gim">Jeong-An Gim</name><affiliation><nlm:aff id="af1-molce-40-4-262">Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 46241,<country>Korea</country></nlm:aff></affiliation><affiliation><nlm:aff id="af2-molce-40-4-262">Genetic Engineering Institute, Pusan National University, Busan 46241,<country>Korea</country></nlm:aff></affiliation><affiliation><nlm:aff id="af3-molce-40-4-262">The Genomics Institute, Life Sciences Department, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919,<country>Korea</country></nlm:aff></affiliation></author><author><name sortKey="Kim, Heui Soo" sort="Kim, Heui Soo" uniqKey="Kim H" first="Heui-Soo" last="Kim">Heui-Soo Kim</name><affiliation><nlm:aff id="af1-molce-40-4-262">Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 46241,<country>Korea</country></nlm:aff></affiliation><affiliation><nlm:aff id="af2-molce-40-4-262">Genetic Engineering Institute, Pusan National University, Busan 46241,<country>Korea</country></nlm:aff></affiliation></author></analytic><series><title level="j">Molecules and Cells</title><idno type="ISSN">1016-8478</idno><idno type="eISSN">0219-1032</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>MicroRNAs (miRNAs) are single-stranded, small RNAs (21–23 nucleotides) that function in gene silencing and translational inhibition via the RNA interference mechanism. Most miRNAs originate from host genomic regions, such as intergenic regions, introns, exons, and transposable elements (TEs). Here, we focused on the palindromic structure of medium reiteration frequencies (MERs), which are similar to precursor miRNAs. Five MER consensus sequences (MER5A1, MER53, MER81, MER91C, and MER117) were matched with paralogous transcripts predicted to be precursor miRNAs in the horse genome (equCab2) and located in either intergenic regions or introns. The MER5A1, MER53, and MER91C sequences obtained from RepeatMasker were matched with the eca-miR-544b, eca-miR-1302, and eca-miR-652 precursor sequences derived from Ensembl transcript database, respectively. Each precursor form was anticipated to yield two mature forms, and we confirmed miRNA expression in six different tissues (cerebrum, cerebellum, lung, spleen, adrenal gland, and duodenum) of one thorough-bred horse. MER5A1-derived miRNAs generally showed significantly higher expression in the lung than in other tissues. MER91C-derived miRNA-5p also showed significantly higher expression in the duodenum than in other tissues (cerebellum, lung, spleen, and adrenal gland). The MER117-overlapped expressed sequence tag generated polycistronic miRNAs, which showed higher expression in the duodenum than other tissues. These data indicate that horse MER transposons encode miR-NAs that are expressed in several tissues and are thought to have biological functions.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Ahn, K" uniqKey="Ahn K">K. Ahn</name></author><author><name sortKey="Gim, J A" uniqKey="Gim J">J.A. Gim</name></author><author><name sortKey="Ha, H S" uniqKey="Ha H">H.S. Ha</name></author><author><name sortKey="Han, K" uniqKey="Han K">K. Han</name></author><author><name sortKey="Kim, H S" uniqKey="Kim H">H.S. Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Altuvia, Y" uniqKey="Altuvia Y">Y. Altuvia</name></author><author><name sortKey="Landgraf, P" uniqKey="Landgraf P">P. Landgraf</name></author><author><name sortKey="Lithwick, G" uniqKey="Lithwick G">G. Lithwick</name></author><author><name sortKey="Elefant, N" uniqKey="Elefant N">N. Elefant</name></author><author><name sortKey="Pfeffer, S" uniqKey="Pfeffer S">S. Pfeffer</name></author><author><name sortKey="Aravin, A" uniqKey="Aravin A">A. Aravin</name></author><author><name sortKey="Brownstein, M J" uniqKey="Brownstein M">M.J. Brownstein</name></author><author><name sortKey="Tuschl, T" uniqKey="Tuschl T">T. Tuschl</name></author><author><name sortKey="Margalit, H" uniqKey="Margalit H">H. Margalit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Balada, E" uniqKey="Balada E">E. Balada</name></author><author><name sortKey="Vilardell Tarres, M" uniqKey="Vilardell Tarres M">M. Vilardell-Tarres</name></author><author><name sortKey="Ordi Ros, J" uniqKey="Ordi Ros J">J. Ordi-Ros</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bartel, D P" uniqKey="Bartel D">D.P. Bartel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Borchert, G M" uniqKey="Borchert G">G.M. Borchert</name></author><author><name sortKey="Holton, N W" uniqKey="Holton N">N.W. Holton</name></author><author><name sortKey="Williams, J D" uniqKey="Williams J">J.D. Williams</name></author><author><name sortKey="Hernan, W L" uniqKey="Hernan W">W.L. Hernan</name></author><author><name sortKey="Bishop, I P" uniqKey="Bishop I">I.P. Bishop</name></author><author><name sortKey="Dembosky, J A" uniqKey="Dembosky J">J.A. Dembosky</name></author><author><name sortKey="Elste, J E" uniqKey="Elste J">J.E. Elste</name></author><author><name sortKey="Gregoire, N S" uniqKey="Gregoire N">N.S. Gregoire</name></author><author><name sortKey="Kim, J A" uniqKey="Kim J">J.A. Kim</name></author><author><name sortKey="Koehler, W W" uniqKey="Koehler W">W.W. Koehler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, K" uniqKey="Chen K">K. Chen</name></author><author><name sortKey="Rajewsky, N" uniqKey="Rajewsky N">N. Rajewsky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Coffin, J M" uniqKey="Coffin J">J.M. Coffin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Doan, R" uniqKey="Doan R">R. Doan</name></author><author><name sortKey="Cohen, N" uniqKey="Cohen N">N. Cohen</name></author><author><name sortKey="Harrington, J" uniqKey="Harrington J">J. Harrington</name></author><author><name sortKey="Veazey, K" uniqKey="Veazey K">K. Veazey</name></author><author><name sortKey="Juras, R" uniqKey="Juras R">R. Juras</name></author><author><name sortKey="Cothran, G" uniqKey="Cothran G">G. Cothran</name></author><author><name sortKey="Mccue, M E" uniqKey="Mccue M">M.E. McCue</name></author><author><name sortKey="Skow, L" uniqKey="Skow L">L. Skow</name></author><author><name sortKey="Dindot, S V" uniqKey="Dindot S">S.V. Dindot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fang, R" uniqKey="Fang R">R. Fang</name></author><author><name sortKey="Olds, L C" uniqKey="Olds L">L.C. Olds</name></author><author><name sortKey="Sibley, E" uniqKey="Sibley E">E. Sibley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Feschotte, C" uniqKey="Feschotte C">C. Feschotte</name></author><author><name sortKey="Swamy, L" uniqKey="Swamy L">L. Swamy</name></author><author><name sortKey="Wessler, S R" uniqKey="Wessler S">S.R. Wessler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fu, H J" uniqKey="Fu H">H.J. Fu</name></author><author><name sortKey="Zhu, J" uniqKey="Zhu J">J. Zhu</name></author><author><name sortKey="Yang, M" uniqKey="Yang M">M. Yang</name></author><author><name sortKey="Zhang, Z Y" uniqKey="Zhang Z">Z.Y. Zhang</name></author><author><name sortKey="Tie, Y" uniqKey="Tie Y">Y. Tie</name></author><author><name sortKey="Jiang, H" uniqKey="Jiang H">H. Jiang</name></author><author><name sortKey="Sun, Z X" uniqKey="Sun Z">Z.X. Sun</name></author><author><name sortKey="Zheng, X F" uniqKey="Zheng X">X.F. Zheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gim, J A" uniqKey="Gim J">J.-A. Gim</name></author><author><name sortKey="Ha, H S" uniqKey="Ha H">H.-S. Ha</name></author><author><name sortKey="Ahn, K" uniqKey="Ahn K">K. Ahn</name></author><author><name sortKey="Kim, D S" uniqKey="Kim D">D.-S. Kim</name></author><author><name sortKey="Kim, H S" uniqKey="Kim H">H.-S. Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gim, J A" uniqKey="Gim J">J.A. Gim</name></author><author><name sortKey="Hong, C P" uniqKey="Hong C">C.P. Hong</name></author><author><name sortKey="Kim, D S" uniqKey="Kim D">D.S. Kim</name></author><author><name sortKey="Moon, J W" uniqKey="Moon J">J.W. Moon</name></author><author><name sortKey="Choi, Y" uniqKey="Choi Y">Y. Choi</name></author><author><name sortKey="Eo, J" uniqKey="Eo J">J. Eo</name></author><author><name sortKey="Kwon, Y J" uniqKey="Kwon Y">Y.J. Kwon</name></author><author><name sortKey="Lee, J R" uniqKey="Lee J">J.R. Lee</name></author><author><name sortKey="Jung, Y D" uniqKey="Jung Y">Y.D. Jung</name></author><author><name sortKey="Bae, J H" uniqKey="Bae J">J.H. Bae</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hayashita, Y" uniqKey="Hayashita Y">Y. Hayashita</name></author><author><name sortKey="Osada, H" uniqKey="Osada H">H. Osada</name></author><author><name sortKey="Tatematsu, Y" uniqKey="Tatematsu Y">Y. Tatematsu</name></author><author><name sortKey="Yamada, H" uniqKey="Yamada H">H. Yamada</name></author><author><name sortKey="Yanagisawa, K" uniqKey="Yanagisawa K">K. Yanagisawa</name></author><author><name sortKey="Tomida, S" uniqKey="Tomida S">S. Tomida</name></author><author><name sortKey="Yatabe, Y" uniqKey="Yatabe Y">Y. Yatabe</name></author><author><name sortKey="Kawahara, K" uniqKey="Kawahara K">K. Kawahara</name></author><author><name sortKey="Sekido, Y" uniqKey="Sekido Y">Y. Sekido</name></author><author><name sortKey="Takahashi, T" uniqKey="Takahashi T">T. Takahashi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hill, E W" uniqKey="Hill E">E.W. Hill</name></author><author><name sortKey="Gu, J" uniqKey="Gu J">J. Gu</name></author><author><name sortKey="Mcgivney, B A" uniqKey="Mcgivney B">B.A. McGivney</name></author><author><name sortKey="Machugh, D E" uniqKey="Machugh D">D.E. MacHugh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hubbard, T" uniqKey="Hubbard T">T. Hubbard</name></author><author><name sortKey="Barker, D" uniqKey="Barker D">D. Barker</name></author><author><name sortKey="Birney, E" uniqKey="Birney E">E. Birney</name></author><author><name sortKey="Cameron, G" uniqKey="Cameron G">G. Cameron</name></author><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y. Chen</name></author><author><name sortKey="Clark, L" uniqKey="Clark L">L. Clark</name></author><author><name sortKey="Cox, T" uniqKey="Cox T">T. Cox</name></author><author><name sortKey="Cuff, J" uniqKey="Cuff J">J. Cuff</name></author><author><name sortKey="Curwen, V" uniqKey="Curwen V">V. Curwen</name></author><author><name sortKey="Down, T" uniqKey="Down T">T. Down</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hutvagner, G" uniqKey="Hutvagner G">G. Hutvagner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jurka, J" uniqKey="Jurka J">J. Jurka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jurka, J" uniqKey="Jurka J">J. Jurka</name></author><author><name sortKey="Kapitonov, V V" uniqKey="Kapitonov V">V.V. Kapitonov</name></author><author><name sortKey="Klonowski, P" uniqKey="Klonowski P">P. Klonowski</name></author><author><name sortKey="Walichiewicz, J" uniqKey="Walichiewicz J">J. Walichiewicz</name></author><author><name sortKey="Smit, A F" uniqKey="Smit A">A.F. Smit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jurka, J" uniqKey="Jurka J">J. Jurka</name></author><author><name sortKey="Kapitonov, V V" uniqKey="Kapitonov V">V.V. Kapitonov</name></author><author><name sortKey="Pavlicek, A" uniqKey="Pavlicek A">A. Pavlicek</name></author><author><name sortKey="Klonowski, P" uniqKey="Klonowski P">P. Klonowski</name></author><author><name sortKey="Kohany, O" uniqKey="Kohany O">O. Kohany</name></author><author><name sortKey="Walichiewicz, J" uniqKey="Walichiewicz J">J. Walichiewicz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, M C" uniqKey="Kim M">M.C. Kim</name></author><author><name sortKey="Lee, S W" uniqKey="Lee S">S.W. Lee</name></author><author><name sortKey="Ryu, D Y" uniqKey="Ryu D">D.Y. Ryu</name></author><author><name sortKey="Cui, F J" uniqKey="Cui F">F.J. Cui</name></author><author><name sortKey="Bhak, J" uniqKey="Bhak J">J. Bhak</name></author><author><name sortKey="Kim, Y" uniqKey="Kim Y">Y. Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kozomara, A" uniqKey="Kozomara A">A. Kozomara</name></author><author><name sortKey="Griffiths Jones, S" uniqKey="Griffiths Jones S">S. Griffiths-Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lamprecht, B" uniqKey="Lamprecht B">B. Lamprecht</name></author><author><name sortKey="Walter, K" uniqKey="Walter K">K. Walter</name></author><author><name sortKey="Kreher, S" uniqKey="Kreher S">S. Kreher</name></author><author><name sortKey="Kumar, R" uniqKey="Kumar R">R. Kumar</name></author><author><name sortKey="Hummel, M" uniqKey="Hummel M">M. Hummel</name></author><author><name sortKey="Lenze, D" uniqKey="Lenze D">D. Lenze</name></author><author><name sortKey="Kochert, K" uniqKey="Kochert K">K. Kochert</name></author><author><name sortKey="Bouhlel, M A" uniqKey="Bouhlel M">M.A. Bouhlel</name></author><author><name sortKey="Richter, J" uniqKey="Richter J">J. Richter</name></author><author><name sortKey="Soler, E" uniqKey="Soler E">E. Soler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lander, E S" uniqKey="Lander E">E.S. Lander</name></author><author><name sortKey="Linton, L M" uniqKey="Linton L">L.M. Linton</name></author><author><name sortKey="Birren, B" uniqKey="Birren B">B. Birren</name></author><author><name sortKey="Nusbaum, C" uniqKey="Nusbaum C">C. Nusbaum</name></author><author><name sortKey="Zody, M C" uniqKey="Zody M">M.C. Zody</name></author><author><name sortKey="Baldwin, J" uniqKey="Baldwin J">J. Baldwin</name></author><author><name sortKey="Devon, K" uniqKey="Devon K">K. Devon</name></author><author><name sortKey="Dewar, K" uniqKey="Dewar K">K. Dewar</name></author><author><name sortKey="Doyle, M" uniqKey="Doyle M">M. Doyle</name></author><author><name sortKey="Fitzhugh, W" uniqKey="Fitzhugh W">W. FitzHugh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, Y" uniqKey="Lee Y">Y. Lee</name></author><author><name sortKey="Kim, M" uniqKey="Kim M">M. Kim</name></author><author><name sortKey="Han, J" uniqKey="Han J">J. Han</name></author><author><name sortKey="Yeom, K H" uniqKey="Yeom K">K.H. Yeom</name></author><author><name sortKey="Lee, S" uniqKey="Lee S">S. Lee</name></author><author><name sortKey="Baek, S H" uniqKey="Baek S">S.H. Baek</name></author><author><name sortKey="Kim, V N" uniqKey="Kim V">V.N. Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lein, E S" uniqKey="Lein E">E.S. Lein</name></author><author><name sortKey="Hawrylycz, M J" uniqKey="Hawrylycz M">M.J. Hawrylycz</name></author><author><name sortKey="Ao, N" uniqKey="Ao N">N. Ao</name></author><author><name sortKey="Ayres, M" uniqKey="Ayres M">M. Ayres</name></author><author><name sortKey="Bensinger, A" uniqKey="Bensinger A">A. Bensinger</name></author><author><name sortKey="Bernard, A" uniqKey="Bernard A">A. Bernard</name></author><author><name sortKey="Boe, A F" uniqKey="Boe A">A.F. Boe</name></author><author><name sortKey="Boguski, M S" uniqKey="Boguski M">M.S. Boguski</name></author><author><name sortKey="Brockway, K S" uniqKey="Brockway K">K.S. Brockway</name></author><author><name sortKey="Byrnes, E J" uniqKey="Byrnes E">E.J. Byrnes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Xie, J" uniqKey="Xie J">J. Xie</name></author><author><name sortKey="Xu, X" uniqKey="Xu X">X. Xu</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name></author><author><name sortKey="Ao, F" uniqKey="Ao F">F. Ao</name></author><author><name sortKey="Wan, Y" uniqKey="Wan Y">Y. Wan</name></author><author><name sortKey="Zhu, Y" uniqKey="Zhu Y">Y. Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liang, T" uniqKey="Liang T">T. Liang</name></author><author><name sortKey="Guo, L" uniqKey="Guo L">L. Guo</name></author><author><name sortKey="Liu, C" uniqKey="Liu C">C. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lund, E" uniqKey="Lund E">E. Lund</name></author><author><name sortKey="Dahlberg, J E" uniqKey="Dahlberg J">J.E. Dahlberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nishimura, M" uniqKey="Nishimura M">M. Nishimura</name></author><author><name sortKey="Naito, S" uniqKey="Naito S">S. Naito</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nozawa, M" uniqKey="Nozawa M">M. Nozawa</name></author><author><name sortKey="Miura, S" uniqKey="Miura S">S. Miura</name></author><author><name sortKey="Nei, M" uniqKey="Nei M">M. Nei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ota, A" uniqKey="Ota A">A. Ota</name></author><author><name sortKey="Tagawa, H" uniqKey="Tagawa H">H. Tagawa</name></author><author><name sortKey="Karnan, S" uniqKey="Karnan S">S. Karnan</name></author><author><name sortKey="Tsuzuki, S" uniqKey="Tsuzuki S">S. Tsuzuki</name></author><author><name sortKey="Karpas, A" uniqKey="Karpas A">A. Karpas</name></author><author><name sortKey="Kira, S" uniqKey="Kira S">S. Kira</name></author><author><name sortKey="Yoshida, Y" uniqKey="Yoshida Y">Y. Yoshida</name></author><author><name sortKey="Seto, M" uniqKey="Seto M">M. Seto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ou Yang, F" uniqKey="Ou Yang F">F. Ou-Yang</name></author><author><name sortKey="Luo, Q J" uniqKey="Luo Q">Q.-J. Luo</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y. Zhang</name></author><author><name sortKey="Richardson, C R" uniqKey="Richardson C">C.R. Richardson</name></author><author><name sortKey="Jiang, Y" uniqKey="Jiang Y">Y. Jiang</name></author><author><name sortKey="Rock, C D" uniqKey="Rock C">C.D. Rock</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pace, J K" uniqKey="Pace J">J.K. Pace</name></author><author><name sortKey="Feschotte, C" uniqKey="Feschotte C">C. Feschotte</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Park, S J" uniqKey="Park S">S.J. Park</name></author><author><name sortKey="Kim, Y H" uniqKey="Kim Y">Y.H. Kim</name></author><author><name sortKey="Lee, S R" uniqKey="Lee S">S.R. Lee</name></author><author><name sortKey="Choe, S H" uniqKey="Choe S">S.H. Choe</name></author><author><name sortKey="Kim, M J" uniqKey="Kim M">M.J. Kim</name></author><author><name sortKey="Kim, S U" uniqKey="Kim S">S.U. Kim</name></author><author><name sortKey="Kim, J S" uniqKey="Kim J">J.S. Kim</name></author><author><name sortKey="Sim, B W" uniqKey="Sim B">B.W. Sim</name></author><author><name sortKey="Song, B S" uniqKey="Song B">B.S. Song</name></author><author><name sortKey="Jeong, K J" uniqKey="Jeong K">K.J. Jeong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Petersen, J L" uniqKey="Petersen J">J.L. Petersen</name></author><author><name sortKey="Mickelson, J R" uniqKey="Mickelson J">J.R. Mickelson</name></author><author><name sortKey="Rendahl, A K" uniqKey="Rendahl A">A.K. Rendahl</name></author><author><name sortKey="Valberg, S J" uniqKey="Valberg S">S.J. Valberg</name></author><author><name sortKey="Andersson, L S" uniqKey="Andersson L">L.S. Andersson</name></author><author><name sortKey="Axelsson, J" uniqKey="Axelsson J">J. Axelsson</name></author><author><name sortKey="Bailey, E" uniqKey="Bailey E">E. Bailey</name></author><author><name sortKey="Bannasch, D" uniqKey="Bannasch D">D. Bannasch</name></author><author><name sortKey="Binns, M M" uniqKey="Binns M">M.M. Binns</name></author><author><name sortKey="Borges, A S" uniqKey="Borges A">A.S. Borges</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Piriyapongsa, J" uniqKey="Piriyapongsa J">J. Piriyapongsa</name></author><author><name sortKey="Jordan, I K" uniqKey="Jordan I">I.K. Jordan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Piriyapongsa, J" uniqKey="Piriyapongsa J">J. Piriyapongsa</name></author><author><name sortKey="Jordan, I K" uniqKey="Jordan I">I.K. Jordan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Piriyapongsa, J" uniqKey="Piriyapongsa J">J. Piriyapongsa</name></author><author><name sortKey="Marino Ramirez, L" uniqKey="Marino Ramirez L">L. Marino-Ramirez</name></author><author><name sortKey="Jordan, I K" uniqKey="Jordan I">I.K. Jordan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ruike, Y" uniqKey="Ruike Y">Y. Ruike</name></author><author><name sortKey="Ichimura, A" uniqKey="Ichimura A">A. Ichimura</name></author><author><name sortKey="Tsuchiya, S" uniqKey="Tsuchiya S">S. Tsuchiya</name></author><author><name sortKey="Shimizu, K" uniqKey="Shimizu K">K. Shimizu</name></author><author><name sortKey="Kunimoto, R" uniqKey="Kunimoto R">R. Kunimoto</name></author><author><name sortKey="Okuno, Y" uniqKey="Okuno Y">Y. Okuno</name></author><author><name sortKey="Tsujimoto, G" uniqKey="Tsujimoto G">G. Tsujimoto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shukla, G C" uniqKey="Shukla G">G.C. Shukla</name></author><author><name sortKey="Singh, J" uniqKey="Singh J">J. Singh</name></author><author><name sortKey="Barik, S" uniqKey="Barik S">S. Barik</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smalheiser, N R" uniqKey="Smalheiser N">N.R. Smalheiser</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smalheiser, N R" uniqKey="Smalheiser N">N.R. Smalheiser</name></author><author><name sortKey="Torvik, V I" uniqKey="Torvik V">V.I. Torvik</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smit, A" uniqKey="Smit A">A. Smit</name></author><author><name sortKey="Hubley, R" uniqKey="Hubley R">R. Hubley</name></author><author><name sortKey="Green, P" uniqKey="Green P">P. Green</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smit, A F" uniqKey="Smit A">A.F. Smit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wade, C M" uniqKey="Wade C">C.M. Wade</name></author><author><name sortKey="Giulotto, E" uniqKey="Giulotto E">E. Giulotto</name></author><author><name sortKey="Sigurdsson, S" uniqKey="Sigurdsson S">S. Sigurdsson</name></author><author><name sortKey="Zoli, M" uniqKey="Zoli M">M. Zoli</name></author><author><name sortKey="Gnerre, S" uniqKey="Gnerre S">S. Gnerre</name></author><author><name sortKey="Imsland, F" uniqKey="Imsland F">F. Imsland</name></author><author><name sortKey="Lear, T L" uniqKey="Lear T">T.L. Lear</name></author><author><name sortKey="Adelson, D L" uniqKey="Adelson D">D.L. Adelson</name></author><author><name sortKey="Bailey, E" uniqKey="Bailey E">E. Bailey</name></author><author><name sortKey="Bellone, R R" uniqKey="Bellone R">R.R. Bellone</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, Z" uniqKey="Yuan Z">Z. Yuan</name></author><author><name sortKey="Sun, X" uniqKey="Sun X">X. Sun</name></author><author><name sortKey="Jiang, D" uniqKey="Jiang D">D. Jiang</name></author><author><name sortKey="Ding, Y" uniqKey="Ding Y">Y. Ding</name></author><author><name sortKey="Lu, Z" uniqKey="Lu Z">Z. Lu</name></author><author><name sortKey="Gong, L" uniqKey="Gong L">L. Gong</name></author><author><name sortKey="Liu, H" uniqKey="Liu H">H. Liu</name></author><author><name sortKey="Xie, J" uniqKey="Xie J">J. Xie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, Z" uniqKey="Yuan Z">Z. Yuan</name></author><author><name sortKey="Sun, X" uniqKey="Sun X">X. Sun</name></author><author><name sortKey="Liu, H" uniqKey="Liu H">H. Liu</name></author><author><name sortKey="Xie, J" uniqKey="Xie J">J. Xie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q. Zhang</name></author><author><name sortKey="Arbuckle, J" uniqKey="Arbuckle J">J. Arbuckle</name></author><author><name sortKey="Wessler, S R" uniqKey="Wessler S">S.R. Wessler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, H" uniqKey="Zhang H">H. Zhang</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y. Liu</name></author><author><name sortKey="Su, D" uniqKey="Su D">D. Su</name></author><author><name sortKey="Yang, Y" uniqKey="Yang Y">Y. Yang</name></author><author><name sortKey="Bai, G" uniqKey="Bai G">G. Bai</name></author><author><name sortKey="Tao, D" uniqKey="Tao D">D. Tao</name></author><author><name sortKey="Ma, Y" uniqKey="Ma Y">Y. Ma</name></author><author><name sortKey="Zhang, S" uniqKey="Zhang S">S. Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, M" uniqKey="Zhou M">M. Zhou</name></author><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q. Wang</name></author><author><name sortKey="Sun, J" uniqKey="Sun J">J. Sun</name></author><author><name sortKey="Li, X" uniqKey="Li X">X. Li</name></author><author><name sortKey="Xu, L" uniqKey="Xu L">L. Xu</name></author><author><name sortKey="Yang, H" uniqKey="Yang H">H. Yang</name></author><author><name sortKey="Shi, H" uniqKey="Shi H">H. Shi</name></author><author><name sortKey="Ning, S" uniqKey="Ning S">S. Ning</name></author><author><name sortKey="Chen, L" uniqKey="Chen L">L. Chen</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Mol Cells</journal-id><journal-id journal-id-type="iso-abbrev">Mol. Cells</journal-id><journal-id journal-id-type="publisher-id">ksmcb</journal-id><journal-title-group><journal-title>Molecules and Cells</journal-title></journal-title-group><issn pub-type="ppub">1016-8478</issn><issn pub-type="epub">0219-1032</issn><publisher><publisher-name>Korean Society for Molecular and Cellular Biology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28320202</article-id><article-id pub-id-type="pmc">5424272</article-id><article-id pub-id-type="doi">10.14348/molcells.2017.2295</article-id><article-id pub-id-type="publisher-id">molce-40-4-262</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Identification and Expression of Equine MER-Derived miRNAs</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Gim</surname><given-names>Jeong-An</given-names></name><xref ref-type="aff" rid="af1-molce-40-4-262">1</xref><xref ref-type="aff" rid="af2-molce-40-4-262">2</xref><xref ref-type="aff" rid="af3-molce-40-4-262">3</xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Heui-Soo</given-names></name><xref ref-type="aff" rid="af1-molce-40-4-262">1</xref><xref ref-type="aff" rid="af2-molce-40-4-262">2</xref><xref rid="c1-molce-40-4-262" ref-type="corresp">*</xref></contrib></contrib-group><aff id="af1-molce-40-4-262"><label>1</label>Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 46241,<country>Korea</country></aff><aff id="af2-molce-40-4-262"><label>2</label>Genetic Engineering Institute, Pusan National University, Busan 46241,<country>Korea</country></aff><aff id="af3-molce-40-4-262"><label>3</label>The Genomics Institute, Life Sciences Department, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919,<country>Korea</country></aff><author-notes><corresp id="c1-molce-40-4-262"><label>*</label>Correspondence: <email>khs307@pusan.ac.kr</email></corresp></author-notes><pub-date pub-type="ppub"><day>30</day><month>4</month><year>2017</year></pub-date><pub-date pub-type="epub"><day>21</day><month>3</month><year>2017</year></pub-date><pub-date pub-type="pmc-release"><day>21</day><month>3</month><year>2017</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>40</volume><issue>4</issue><fpage>262</fpage><lpage>270</lpage><history><date date-type="received"><day>04</day><month>12</month><year>2016</year></date><date date-type="rev-recd"><day>06</day><month>3</month><year>2017</year></date><date date-type="accepted"><day>15</day><month>3</month><year>2017</year></date></history><permissions><copyright-statement>© The Korean Society for Molecular and Cellular Biology. All rights reserved.</copyright-statement><copyright-year>2017</copyright-year><license><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0/">http://creativecommons.org/licenses/by-nc-sa/3.0/</ext-link>.</license-p></license></permissions><abstract><p>MicroRNAs (miRNAs) are single-stranded, small RNAs (21–23 nucleotides) that function in gene silencing and translational inhibition via the RNA interference mechanism. Most miRNAs originate from host genomic regions, such as intergenic regions, introns, exons, and transposable elements (TEs). Here, we focused on the palindromic structure of medium reiteration frequencies (MERs), which are similar to precursor miRNAs. Five MER consensus sequences (MER5A1, MER53, MER81, MER91C, and MER117) were matched with paralogous transcripts predicted to be precursor miRNAs in the horse genome (equCab2) and located in either intergenic regions or introns. The MER5A1, MER53, and MER91C sequences obtained from RepeatMasker were matched with the eca-miR-544b, eca-miR-1302, and eca-miR-652 precursor sequences derived from Ensembl transcript database, respectively. Each precursor form was anticipated to yield two mature forms, and we confirmed miRNA expression in six different tissues (cerebrum, cerebellum, lung, spleen, adrenal gland, and duodenum) of one thorough-bred horse. MER5A1-derived miRNAs generally showed significantly higher expression in the lung than in other tissues. MER91C-derived miRNA-5p also showed significantly higher expression in the duodenum than in other tissues (cerebellum, lung, spleen, and adrenal gland). The MER117-overlapped expressed sequence tag generated polycistronic miRNAs, which showed higher expression in the duodenum than other tissues. These data indicate that horse MER transposons encode miR-NAs that are expressed in several tissues and are thought to have biological functions.</p></abstract><kwd-group><kwd>medium reiteration frequency transposon</kwd><kwd>MicroRNA</kwd><kwd>palindromic structure</kwd><kwd>thoroughbred horse</kwd></kwd-group></article-meta></front><body><sec sec-type="intro"><title>INTRODUCTION</title><p>MicroRNAs (miRNAs) are non-coding small RNAs (21–23 nucleotides) that play a key role in inhibiting target genes by binding their 3′ UTRs, which are complementary to the miR-NAs’ seed regions (<xref rid="b4-molce-40-4-262" ref-type="bibr">Bartel, 2009</xref>; <xref rid="b6-molce-40-4-262" ref-type="bibr">Chen and Rajewsky, 2007</xref>; <xref rid="b41-molce-40-4-262" ref-type="bibr">Shukla et al., 2011</xref>). These sequences are known to influence various biological processes in numerous animals and plants (<xref rid="b4-molce-40-4-262" ref-type="bibr">Bartel, 2009</xref>). miRNAs undergo a series of changes from the transcript stage to maturity. First, primary miRNAs are transcribed by polymerase II in the nucleus (<xref rid="b25-molce-40-4-262" ref-type="bibr">Lee et al., 2004</xref>). Next, they are cleaved into precursor miRNAs (70 nucleotides), with a double-stranded hairpin structure, by Drosha. Finally, precursor miRNAs are exported into the cytoplasm and converted to mature miRNAs by Dicer (<xref rid="b29-molce-40-4-262" ref-type="bibr">Lund and Dahlberg, 2006</xref>). In a genome, miRNA-encoding genes are located in various characterized regions such as intergenic regions, exons, introns, and transposable elements (TEs) (<xref rid="b39-molce-40-4-262" ref-type="bibr">Piriyapongsa et al., 2007</xref>). TEs contribute to miRNA sequences in various ways. For example, two adjacent TEs, as well as genomic sequences and one TE, yield one precursor miRNA. Palindrome sequences containing TEs such as miniature inverted repeat transposable elements (MITE) and medium reiteration frequency (MER) generate one precursor form and two mature miRNAs (<xref rid="b1-molce-40-4-262" ref-type="bibr">Ahn et al., 2013</xref>; <xref rid="b47-molce-40-4-262" ref-type="bibr">Yuan et al., 2010</xref>). Two mature miRNAs are derived from one precursor miRNA and present asymmetric expression or selective expression patterns (<xref rid="b17-molce-40-4-262" ref-type="bibr">Hutvagner, 2005</xref>; <xref rid="b40-molce-40-4-262" ref-type="bibr">Ruike et al., 2008</xref>).</p><p>MERs are non-autonomous mammalian DNA transposons (<xref rid="b19-molce-40-4-262" ref-type="bibr">Jurka et al., 1996</xref>). A total of 240,000 copies of MERs are present in humans, accounting for 2.4% of the human genome (<xref rid="b24-molce-40-4-262" ref-type="bibr">Lander et al., 2001</xref>). Most of these sequences were fossilized, such as long terminal repeats (LTRs) or endogenous retroviruses (ERVs) (<xref rid="b7-molce-40-4-262" ref-type="bibr">Coffin, 2004</xref>; <xref rid="b45-molce-40-4-262" ref-type="bibr">Smit, 1993</xref>), although some cause diseases in host organisms (<xref rid="b3-molce-40-4-262" ref-type="bibr">Balada et al., 2010</xref>; <xref rid="b23-molce-40-4-262" ref-type="bibr">Lamprecht et al., 2010</xref>). The genomes of primates, rodentia, and lagomorpha contain MER repeats inserted before the <italic>Alu</italic> family genes (<xref rid="b19-molce-40-4-262" ref-type="bibr">Jurka et al., 1996</xref>). MITEs are a truncated form of autonomous DNA transposons (<xref rid="b10-molce-40-4-262" ref-type="bibr">Feschotte et al., 2003</xref>; <xref rid="b49-molce-40-4-262" ref-type="bibr">Zhang et al., 2000</xref>). Notably, MER or MITE sequences have palindromic structures, which form hairpins, similar to precursor miRNAs. Therefore, palindromic MERs or MITEs can give rise to miRNAs when they are located in transcriptionally active genomic regions. The MER53 transposon is predicted to give rise to miRNAs by forming a palindromic structure in mammalian genomes (<xref rid="b47-molce-40-4-262" ref-type="bibr">Yuan et al., 2010</xref>). The expression of MER-derived miRNAs was experimentally confirmed in human cell lines (<xref rid="b1-molce-40-4-262" ref-type="bibr">Ahn et al., 2013</xref>). MITE-derived miRNAs were predicted (<xref rid="b37-molce-40-4-262" ref-type="bibr">Piriyapongsa and Jordan, 2007</xref>), and their expression confirmed in rice (<xref rid="b33-molce-40-4-262" ref-type="bibr">Ou-Yang et al., 2013</xref>).</p><p>The reference genome sequence for horse was determined in 2007, and the horse genetic map has been available since 2009 (<xref rid="b46-molce-40-4-262" ref-type="bibr">Wade et al., 2009</xref>). The horse genome contains numerous genetic traits linked to racing ability, such as single-nucleotide polymorphisms (SNPs), specific transcripts, and copy number variation (CNV) (<xref rid="b8-molce-40-4-262" ref-type="bibr">Doan et al., 2012</xref>; <xref rid="b15-molce-40-4-262" ref-type="bibr">Hill et al., 2010</xref>; <xref rid="b36-molce-40-4-262" ref-type="bibr">Petersen et al., 2013</xref>). Based on the horse reference genome sequence, a total of 1397 horse miRNAs have been identified in the miRBase database (<xref rid="b22-molce-40-4-262" ref-type="bibr">Kozomara and Griffiths-Jones, 2014</xref>). Subsequently, the functional study and characterization of horse miRNAs could be available (<xref rid="b21-molce-40-4-262" ref-type="bibr">Kim et al., 2014</xref>; <xref rid="b51-molce-40-4-262" ref-type="bibr">Zhou et al., 2009</xref>). However, horse miR-NAs have not been as relatively well studied as other genetic traits, such as SNPs or CNV (<xref rid="b8-molce-40-4-262" ref-type="bibr">Doan et al., 2012</xref>; <xref rid="b13-molce-40-4-262" ref-type="bibr">Gim et al., 2015</xref>; <xref rid="b15-molce-40-4-262" ref-type="bibr">Hill et al., 2010</xref>).</p><p>Many identified expressed sequence tags (ESTs) are available in databases, and ESTs can encode miRNAs and miRNA clusters (<xref rid="b42-molce-40-4-262" ref-type="bibr">Smalheiser, 2003</xref>). The miRNA cluster transcripts are formed from ESTs, which show polycistronic expression patterns containing multiple, distinct loops separated by Drosha and Dicer. Forty-eight percent of all human mature miRNAs are derived from primary miRNAs of polycistronic transcripts (<xref rid="b2-molce-40-4-262" ref-type="bibr">Altuvia et al., 2005</xref>). A well-known miRNA cluster, previously known as <italic>C13orf25</italic>, miR-17-92, can express six precursor miRNAs and is more highly expressed in B-cell lymphoma cells derived from both cell lines or patients than in normal individuals (<xref rid="b32-molce-40-4-262" ref-type="bibr">Ota et al., 2004</xref>). In the present study, we predicted and experimentally confirmed the expression of equine MER-derived miRNAs, including a miRNA cluster in a previously identified EST. We searched consensus MER elements predicted to have palindromic structures and compared the MER sequences to horse transcripts. Next, we experimentally confirmed the expression of horse transcripts showing overlap with MER sequences. Finally, we compared the expression levels of MER-derived miRNAs in various tissues of one thoroughbred horse.</p></sec><sec sec-type="methods"><title>MATERIALS AND METHODS</title><sec><title>Prediction of MER-derived miRNA sequences by bioinformatics tools</title><p>MER consensus sequences that form palindromic sequences were downloaded from the Repbase database version 21.05 of the Genetic Information Research Institute (<ext-link ext-link-type="uri" xlink:href="http://www.girinst.org/repbase/">http://www.girinst.org/repbase/</ext-link>) (<xref rid="b20-molce-40-4-262" ref-type="bibr">Jurka et al., 2005</xref>). The genomic locations of MER sequences were confirmed in the horse genome (Broad/equCab2), using UCSC Genome Browser (<ext-link ext-link-type="uri" xlink:href="http://genome.ucsc.edu">http://genome.ucsc.edu</ext-link>), and their sequences were analyzed to identify regions that overlapped with transcripts, using Ensembl 84 (<ext-link ext-link-type="uri" xlink:href="http://www.ensembl.org">http://www.ensembl.org</ext-link>). In the UCSC table Browser, MER sequences and their genomic regions were compared using RepeatMasker 3.3 (<ext-link ext-link-type="uri" xlink:href="http://www.repeatmasker.org">http://www.repeatmasker.org</ext-link>). The sequences of horse precursor miRNAs and mature miRNAs were downloaded from miRBase v21.0 (<ext-link ext-link-type="uri" xlink:href="http://www.mirbase.org">http://www.mirbase.org</ext-link>), then their sequences were mapped and localized to the horse genome (equCab2).</p><p>MER consensus sequences forming palindromic sequences were predicted by RNAfold (<ext-link ext-link-type="uri" xlink:href="http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi">http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi</ext-link>). To identify potential MER-derived miRNA genes, we predicted the miRNA precursor forms originating from MER elements, using the miRNAfold tool (<ext-link ext-link-type="uri" xlink:href="http://evryrna.ibisc.univ-evry.fr/miRNAFold/">http://evryrna.ibisc.univ-evry.fr/miRNAFold/</ext-link>) for the horse genome. Next, mature miRNAs derived from MER precursor forms (selected for having minimum free energies) were predicted using MatureBayes program (<ext-link ext-link-type="uri" xlink:href="http://mirna.imbb.forth.gr/Mature-Bayes.html">http://mirna.imbb.forth.gr/Mature-Bayes.html</ext-link>). MatureBayes is a tool to predict mature miRNA from miRNA precursor palindromic hairpin structures. Mature forms were confirmed by RT-PCR, we used these miRNAs for expression profiling in six tissues of one horse. Strategy for transcripts analysis in MER-derived horse miRNAs were described in <xref rid="s1-molce-40-4-262" ref-type="supplementary-material">Supplementary Fig. 1</xref>.</p></sec><sec><title>RNA samples</title><p>The animal protocol and sample extraction method in this study were reviewed by the Pusan National University-Institutional Animal Care and Use Committee (PNU-IACUC) for the ethical procedures and scientific care (approval number PNU-2013-0411). Small RNA from 100 mg of different tissues from one thoroughbred horse, sacrificed for biological research, was extracted from the cerebrum, cerebellum, lung, spleen, adrenal gland, and duodenum, using Hybrid-R™ miRNA (GeneAll Biotechnology Co., Ltd., Korea) according to the manufacturer’s protocol. Hybrid-R™ kit is available to simultaneously isolate large RNA and small RNA, and to separate total RNA and small RNA by using small RNA specific binding column tube. In order to exclude short RNA sequences from degreaded longer transcripts, a portion (2–3μl) of total RNA was loaded onto an agarose gel, then only detected 28S and 18S samples were used.</p><p>Next, the turbo DNA-free™ kit (Ambion, USA) was used according to the manufacturer’s protocol to remove DNA contamination from the small RNAs. A portion (2–3 μl) of isolated small RNA was loaded onto an agarose gel, and only samples in which small-RNA (< 200 nt) was detected were used in subsequent experiments. We measured the small RNA concentrations, which ranged between 130–180 ng/μl in the samples. Each small RNA sample was quantitated to 500 ng using a NanoDrop® ND-1000 UV–vis Spectrophotometer (NanoDrop Technologies, USA). After quantifying small RNA, adenine was added to the small RNA transcripts at their 3′ ends, using poly (A) tailing kits (Ambion). Samples were incubated in E-PAP buffer at 37°C for 30 min for the poly (A) tailing reactions. After the reactions, we performed reverse transcription reactions using Moloney murine leukemia virus reverse transcriptase with an RNase inhibitor (Promega, USA) at an annealing temperature of 42°C. To select target miRNAs in the reverse transcription reactions, we used an oligo-dT adaptor (5′-CTGTGAATGCTG CGACTACGAT-18dTs-3′) according to the methods of previous study (<xref rid="b11-molce-40-4-262" ref-type="bibr">Fu et al., 2006</xref>). The miRNA sequence is used as forward primer, and adaptor sequences excluded poly T in the 3′ terminal is used as reverse primer (<xref rid="s1-molce-40-4-262" ref-type="supplementary-material">Supplementary Table S1</xref>).</p></sec><sec><title>Quantitative RT-PCR amplification</title><p>RT-PCR was conducted in a thermocycler (Eppendorf, Germany) and SYBR green quantitative RT-PCR was conducted using a Roter Gene Q (Qiagen, Germany). Each reaction was performed in a final volume of 20 μl containing 1 μl of cDNA sample as a template, 10 μl of Quantitect® SYBR® Green PCR MasterMix (Qiagen), 7 μl of nuclease-free water, and 1μl each of sense primer and antisense primer (each 10 pmol). RT-PCR of genes was performed at 94°C for 5 min, 35 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 30 s, and a final elongation at 72°C for 7 min. Next, quantitative RT-PCR of genes was performed at 94°C for 15 min, 55 cycles of 94°C for 10 s, 55°C for 15 s, and 72°C for 15 s using the primers shown in <xref rid="s1-molce-40-4-262" ref-type="supplementary-material">Supplementary Table 1</xref>. Melting curves were determined by increasing the temperature from 55°C to 99°C over a period of 30 s. Each sample was amplified in triplicate to confirm reproducibility. U6 was used as a positive control in RT-PCR analysis and as a standard control for normalization in qRT-PCR. The relative quantitative levels of MER-derived miRNAs were normalized to U6 small-RNA expression levels. The expression values were determined by the 2<sup>−ΔC</sup><sub>t</sub> method, where ΔC<sub>t</sub> = C<sub>t(miRNA)</sub>−C<sub>t(U6)</sub>.</p></sec></sec><sec sec-type="results"><title>RESULTS</title><sec><title>Prediction of MER-derived miRNAs</title><p>Some of consensus sequences of MER have palindromic structures, which are similar to those of precursor miRNAs. A total of 238 MER elements were screened from the Repbase database, and 24 MER elements were identified to have palindromic structures. In the 24 MER elements, only five MER-derived mature miRNAs were confirmed as RT-PCR (<xref rid="s1-molce-40-4-262" ref-type="supplementary-material">Supplementary Fig. 2</xref>). The others, 19 MER elements were not matched with horse transcripts, or horse genome (equCab2). Therefore, we selected five palindromic MER consensus sequences that matched with horse transcripts (<xref rid="f1-molce-40-4-262" ref-type="fig">Fig. 1</xref>). Each five actual MER sequences is provided as <xref rid="s1-molce-40-4-262" ref-type="supplementary-material">Supplementary Table 2</xref>. Numerous paralogs families matched the MER consensus sequences in horse genomes, based on UCSC BLAT searching (<xref rid="t1-molce-40-4-262" ref-type="table">Table 1</xref>). Six transcripts overlapped with MER5A1 consensus sequences and were matched with eca-miR-544b (<xref rid="b16-molce-40-4-262" ref-type="bibr">Hubbard et al., 2002</xref>; <xref rid="b18-molce-40-4-262" ref-type="bibr">Jurka, 2000</xref>; <xref rid="b44-molce-40-4-262" ref-type="bibr">Smit et al., 2004</xref>). The E-values of all transcripts were below 6.00E-06 based on miRBase BLAST searching and the transcripts contained constructed palindromic structures. Three of six miR-NAs were located in the intronic region, whereas the others were located in the intergenic region. MER53 consensus sequences were matched to two transcripts related to eca-miR-1302-1 and eca-miR-1302c-5. Both miRNAs were located in the intron. Their E-values were 6.00E-08 and 1.00E-15, respectively. One transcript matched a MER91C consensus sequence in the horse transcript and express eca-miR-652 (E-value = 2.00E-34). MER117 was matched to horse EST BM734541.1 (<xref rid="t2-molce-40-4-262" ref-type="table">Table 2</xref>). The BM734541.1 transcript was located in the intron of two transcripts, was predicted to contain many palindromic structures, and may generate 10 precursor miRNAs as putative polycistronic clustered miRNAs (<ext-link ext-link-type="uri" xlink:href="http://www.ncbi.nlm.nih.gov/nucest/BM734541">http://www.ncbi.nlm.nih.gov/nucest/BM734541</ext-link>).</p></sec><sec><title>Validation and quantitative expression patterns of MER-derived miRNAs in a thoroughbred horse</title><p>To confirm that the mature miRNAs were derived from MER transposons, all mature miRNAs were extracted from the tissues of one thoroughbred horse. Adenine and adaptor sequences were added to the 3′ end of each mature miRNA (see “Materials and Methods”). Next, RT-PCR was performed to identify the expression of 24 MER transposon-derived miRNA, and only five miRNAs were detected. Then, we performed qRT-PCR of five miRNAs in the six tissues of one horse (Cerebrum, Cerebellum, Lung, Spleen, Adrenal gland, Duodenum), and MER-derived miRNA showed tissue-specific expression patterns. As shown in <xref rid="f2-molce-40-4-262" ref-type="fig">Fig. 2</xref>, two mature miRNAs derived from one primary miRNA showed similar expression patterns. The MER5A1-derived ENSECAT000000 29718 transcript yielded two miRNAs showing lung-dominant expression patterns than other tissues (<xref rid="f2-molce-40-4-262" ref-type="fig">Fig. 2A</xref>). The MER53-derived ENSECAT00000027640 transcript yielded two miRNAs showing different expression patterns between the -5p and -3p forms. The -5p miRNA showed higher expression value in the cerebrum and duodenum than in other tissues, but no significant expression patterns were detected (<xref rid="f2-molce-40-4-262" ref-type="fig">Fig. 2B</xref>, left). The -3p miRNA showed dominant expression patterns in the duodenum, compared to cerebrum, cerebellum, lung, and adrenal gland (<xref rid="f2-molce-40-4-262" ref-type="fig">Fig. 2B</xref>, right). The MER81-derived ENSECAT00000029221 transcript yielded two miRNAs, that were no significant expression patterns (<xref rid="f2-molce-40-4-262" ref-type="fig">Fig. 2C</xref>). The MER91C-derived ENSECAT00000027964 transcript yielded two miRNAs, which showed different expression patterns. The -5p miRNA was enriched in the duodenum than other tissues (<xref rid="f2-molce-40-4-262" ref-type="fig">Fig. 2D</xref>, left), whereas the -3p miRNA was enriched in the cerebrum than cerebellum, lung, and duodenum (<xref rid="f2-molce-40-4-262" ref-type="fig">Fig. 2D</xref>, right).</p></sec><sec><title>Identification of MER117-derived polycistronic miRNA cluster</title><p>We confirmed that the MER117 sequence overlapped with horse EST of BM734541.1, the transcript of which was predicted to contain many palindromic structures. The BM734541.1 transcript was predicted to generate five precursor forms of polycistronic miRNAs. As shown in <xref rid="f3-molce-40-4-262" ref-type="fig">Fig. 3</xref>, the expression patterns of five different mature forms of the BM734541.1 were confirmed. We detected these mature forms in six different tissues, indicating that total mature forms except EST-2-derived mature form have significantly low expression patterns in the cerebellum than adrenal gland (<xref rid="f3-molce-40-4-262" ref-type="fig">Figs. 3A, 3C–3E</xref>). Interestingly, EST-1-derived mature form was dominantly expressed in other tissues than cerebellum, except cerebrum (<xref rid="f3-molce-40-4-262" ref-type="fig">Fig. 3A</xref>). Only adrenal gland had higher expression pattern than cerebellum in the EST-4-derived mature form (<xref rid="f3-molce-40-4-262" ref-type="fig">Fig. 3D</xref>). The location of mature miR-NAs in the BM734541.1 transcript is indicated for each corresponding region in <xref rid="f3-molce-40-4-262" ref-type="fig">Fig. 3F</xref>.</p></sec></sec><sec sec-type="discussion"><title>DISCUSSION</title><p>MER-sequences are non-autonomous DNA transposons distributed throughout the host genome in a fossilized state. According to pre-RepeatMasking data, the horse genome possesses 3.61% DNA transposons containing MER-sequences (<xref rid="b44-molce-40-4-262" ref-type="bibr">Smit et al., 2004</xref>). MER sequences can form palindromic stem-loop structures, which are similar to miR-NA precursor sequences. This suggests a mode for miRNA sequence formation (<xref rid="b1-molce-40-4-262" ref-type="bibr">Ahn et al., 2013</xref>; <xref rid="b12-molce-40-4-262" ref-type="bibr">Gim et al., 2014</xref>). To date, miRNAs that are derived from repetitive elements have been identified in the genomes of various species (<xref rid="b31-molce-40-4-262" ref-type="bibr">Nozawa et al., 2010</xref>; <xref rid="b48-molce-40-4-262" ref-type="bibr">Yuan et al., 2011</xref>). In this study, we identified 13 MER-derived miRNAs and confirmed their expression in six tissues of one thoroughbred horse.</p><p>During miRNA maturation, two mature miRNAs are processed from one precursor miRNA. According to previous studies, the two mature miRNAs are expressed via asymmetric selection of each miRNA strand by the processing of precursor miRNA (<xref rid="b17-molce-40-4-262" ref-type="bibr">Hutvagner, 2005</xref>; <xref rid="b40-molce-40-4-262" ref-type="bibr">Ruike et al., 2008</xref>). In this study, we also observed that two miRNAs presented different expression patterns. In the MER5A1-derived miRNAs, similar expression patterns were observed for the two miR-NAs from one precursor miRNA (<xref rid="f2-molce-40-4-262" ref-type="fig">Fig. 2A</xref>), whereas the MER53- and MER91C-derived miRNAs showed slightly different expression patterns (<xref rid="f2-molce-40-4-262" ref-type="fig">Figs. 2C and 2D</xref>). MER5A1-derived miRNAs showed dominant expression in the lung; thus, it may be a good target for future studies on exercise, cardiopulmonary fitness, or any lung-related process (<xref rid="f2-molce-40-4-262" ref-type="fig">Fig. 2A</xref>). As a part of brain, cerebrum and cerebellum are related to central nervous system (CNS). However, they expression patterns have different in MER81 and MER91C-derived miRNAs (<xref rid="f2-molce-40-4-262" ref-type="fig">Figs. 2C and 2D</xref>). Cerebrum is most important region of the CNS, and controls all voluntary operation in the body. Thus, these different expression patterns could be related to the cerebrum-specific roles in the CNS. Moreover, these results could provide a clue for cognitive function of the horse (<xref rid="b26-molce-40-4-262" ref-type="bibr">Lein et al., 2007</xref>). Spleen is related to immune response, and adrenal gland produces a variety of hormones. In our data, spleen and adrenal gland have lower expressed patterns. Therefore, these two organs provide the important points in the immune and internal secretion pathways in the organisms (<xref rid="b30-molce-40-4-262" ref-type="bibr">Nishimura and Naito, 2005</xref>). Most chemical digestion takes place in duodenum, therefore MER53-derived ENSECAT00000027640-3p miRNA, MER91C-derived ENSEC AT00000027964-5p miRNA and BM734541.1-derived miR-NAs could be crucial roles in digestion (<xref rid="b9-molce-40-4-262" ref-type="bibr">Fang et al., 2006</xref>). Our six tissue expression patterns could be involved in exercise, cognition, and the physiological pathway.</p><p>MER5A1-derived miRNA precursor sequences were well-matched with eca-miR-544b (<xref rid="t2-molce-40-4-262" ref-type="table">Table 2</xref>); therefore, they may be good targets for future studies on exercise and cardio-pulmonary fitness. miR-544b has, to date, been identified in only three species (human, cow, and horse), and additional studies are required to determine the species- or tissue-specific roles of miR-544b. MER53 has been predicted to encode miR-1302 (<xref rid="b47-molce-40-4-262" ref-type="bibr">Yuan et al., 2010</xref>). MER53-derived miR-NAs did not show consistent expression patterns among their families. The miR-1302 subfamily derived from MER53 showed tissue-specific expression and identified in human, chimpanzee, orangutan (<xref rid="b22-molce-40-4-262" ref-type="bibr">Kozomara and Griffiths-Jones, 2014</xref>). Therefore, it may be interesting to analyze their functions and evolutionary mechanisms in various animals in further studies. MER81-derived miRNAs have no significantly expressed patterns in all tissues to the other miRNAs. Three of four MER81-derived precursor sequences were not matched with previously identified precursor sequences. Although only one sequence matched with eca-miR-8990, its E-value was not significant. According to the latest version of miRBase (version 21.0), a total of 1397 horse miR-NAs have been identified. However, when compared to the total of 4523 human miRNAs, the number of horse miRNAs is small (<xref rid="b22-molce-40-4-262" ref-type="bibr">Kozomara and Griffiths-Jones, 2014</xref>). This means that additional miRNAs may be identified in the horse genome; our data identified some of these miRNAs. Two MER91C-derived precursor miRNAs and four mature miR-NAs were identified, and their expression in human cell lines has been validated (<xref rid="b1-molce-40-4-262" ref-type="bibr">Ahn et al., 2013</xref>). MER91C-derived precursor sequences were matched to eca-miR-652 in the horse (<xref rid="t2-molce-40-4-262" ref-type="table">Table 2</xref>) and to hsa-miR-652 in humans (<xref rid="b1-molce-40-4-262" ref-type="bibr">Ahn et al., 2013</xref>). Many studies have identified TE-derived miRNAs (<xref rid="b5-molce-40-4-262" ref-type="bibr">Borchert et al., 2011</xref>; <xref rid="b39-molce-40-4-262" ref-type="bibr">Piriyapongsa et al., 2007</xref>; <xref rid="b43-molce-40-4-262" ref-type="bibr">Smalheiser and Torvik, 2005</xref>). Specifically, palindromic structures of TEs show the potential to generate miRNA precursor forms; thus, MITE- and MER-derived miRNAs are well-known (<xref rid="b1-molce-40-4-262" ref-type="bibr">Ahn et al., 2013</xref>; <xref rid="b12-molce-40-4-262" ref-type="bibr">Gim et al., 2014</xref>; <xref rid="b38-molce-40-4-262" ref-type="bibr">Piriyapongsa and Jordan, 2008</xref>; <xref rid="b39-molce-40-4-262" ref-type="bibr">Piriyapongsa et al., 2007</xref>; <xref rid="b47-molce-40-4-262" ref-type="bibr">Yuan et al., 2010</xref>). However, few studies have examined the functions of TE-derived miRNAs. We determined the expression patterns in several tissues in a horse, and additional studies are needed to evaluate the functions of these miRNAs.</p><p>We also predicted the miRNA cluster, identified as BM734541.1, in the horse EST <italic>SLC28A3</italic> intron. This EST overlapped with MER117, and we predicted five precursor miRNAs. In eukaryotic protein coding, the gene expression pattern is monocistronic; therefore, one transcript encodes one amino acid chain. However, the polycistronic miRNA cluster forms multiple miRNA components, each of which has a distinct role. miRNA clusters have been identified in the human genome, and miR-17-92 is a well-known polycistronic miRNA that acts as an onco-miRNA. The <italic>C13orf25</italic> gene is chiefly known to show high expression in several hematopoietic malignancies (<xref rid="b32-molce-40-4-262" ref-type="bibr">Ota et al., 2004</xref>). The miR-17-92 cluster is composed of seven mature miRNAs located in the third intron of the <italic>C13orf25</italic> gene in human chromosome 13 and is significantly expressed in lung cancer patients (<xref rid="b14-molce-40-4-262" ref-type="bibr">Hayashita et al., 2005</xref>; <xref rid="b32-molce-40-4-262" ref-type="bibr">Ota et al., 2004</xref>). To date, little is known about the function of the identified short ESTs in the intergenic regions or intron regions, and these ESTs may generate new polycistronic miRNA. In this study, we demonstrated that the EST sequence forms the miRNA cluster and confirmed their polycistronic expression of the miRNA. These results provide a basis for identifying new polycistronic miRNAs from ESTs.</p><p>As an invader of host genomes, TE sequences have undergone rapid evolution compared to other genomic sequences (<xref rid="b35-molce-40-4-262" ref-type="bibr">Park et al., 2015</xref>). As a class II TE, DNA transposons, including MER repeats, underwent arrangement and were then conserved in the host genome (<xref rid="b34-molce-40-4-262" ref-type="bibr">Pace and Feschotte, 2007</xref>). MER-derived miRNAs, detected in humans and horses, may be present in other mammals, including rodentia and lagomorpha. MER5A1-derived miRNAs were matched with miR-544b, which was previously identified in humans, horses, and cows. MER53-matched miR-1302 and MER91C-derived miR-652 were identified in four and fourteen species, respectively (<xref rid="b22-molce-40-4-262" ref-type="bibr">Kozomara and Griffiths-Jones, 2014</xref>). Similarly, many cases of TE-derived miRNAs have been linked to phylogeny-specific miRNAs (<xref rid="b39-molce-40-4-262" ref-type="bibr">Piriyapongsa et al., 2007</xref>). For instance, MITE-derived miR-548 was principally identified in primates (<xref rid="b28-molce-40-4-262" ref-type="bibr">Liang et al., 2012</xref>).</p><p>We predict that additional miRNAs, as well as ESTs, derived from palindrome sequence TEs will be identified. In the host, these miRNAs were reported to have various roles. In a human study, the SNP in miR-1302-binding sites impaired spermatogenesis (<xref rid="b50-molce-40-4-262" ref-type="bibr">Zhang et al., 2011</xref>), and miR-652 expression was related to heart disease. miR-548 is involved in the host antiviral response by targeting interferon λ1, and thus may be related to the immune system (<xref rid="b27-molce-40-4-262" ref-type="bibr">Li et al., 2013</xref>). Based on these results, further studies are required to determine the functions of these miRNAs in horse and other mammalian species.</p><p>Owing to the development of next-generation sequencing technology, more precise genome sequencing data and transcript data is expected to be available. This study provides insights into novel functional transcripts. Future studies are required to understand how TE-derived miRNAs manipulate biological functions.</p></sec><sec sec-type="supplementary-material"><title>Supplementary data</title><supplementary-material content-type="local-data" id="s1-molce-40-4-262"><media xlink:href="molce-40-4-262-supple.pdf" mimetype="application" mime-subtype="pdf" orientation="portrait" id="d35e590" position="anchor"></media></supplementary-material></sec></body><back><ack><title>ACKNOWLEDGMENTS</title><p>This work was supported by a grant from the ministry of Education, Republic of Korea (NRF-2015R1D1A1A01056650).</p></ack><fn-group><fn id="fn1-molce-40-4-262"><p>Note: <xref rid="s1-molce-40-4-262" ref-type="supplementary-material">Supplementary information</xref> is available on the Molecules and Cells website (<ext-link ext-link-type="uri" xlink:href="www.molcells.org">www.molcells.org</ext-link>).</p></fn></fn-group><ref-list><title>REFERENCES</title><ref id="b1-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ahn</surname><given-names>K.</given-names></name><name><surname>Gim</surname><given-names>J.A.</given-names></name><name><surname>Ha</surname><given-names>H.S.</given-names></name><name><surname>Han</surname><given-names>K.</given-names></name><name><surname>Kim</surname><given-names>H.S.</given-names></name></person-group><year>2013</year><article-title>The novel MER transposon-derived miRNAs in human genome</article-title><source>Gene</source><volume>512</volume><fpage>422</fpage><lpage>428</lpage><pub-id pub-id-type="pmid">22926102</pub-id></element-citation></ref><ref id="b2-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Altuvia</surname><given-names>Y.</given-names></name><name><surname>Landgraf</surname><given-names>P.</given-names></name><name><surname>Lithwick</surname><given-names>G.</given-names></name><name><surname>Elefant</surname><given-names>N.</given-names></name><name><surname>Pfeffer</surname><given-names>S.</given-names></name><name><surname>Aravin</surname><given-names>A.</given-names></name><name><surname>Brownstein</surname><given-names>M.J.</given-names></name><name><surname>Tuschl</surname><given-names>T.</given-names></name><name><surname>Margalit</surname><given-names>H.</given-names></name></person-group><year>2005</year><article-title>Clustering and conservation patterns of human microRNAs</article-title><source>Nucleic Acids Res</source><volume>33</volume><fpage>2697</fpage><lpage>2706</lpage><pub-id pub-id-type="pmid">15891114</pub-id></element-citation></ref><ref id="b3-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Balada</surname><given-names>E.</given-names></name><name><surname>Vilardell-Tarres</surname><given-names>M.</given-names></name><name><surname>Ordi-Ros</surname><given-names>J.</given-names></name></person-group><year>2010</year><article-title>Implication of human endogenous retroviruses in the development of autoimmune diseases</article-title><source>Int Rev Immunol</source><volume>29</volume><fpage>351</fpage><lpage>370</lpage><pub-id pub-id-type="pmid">20635879</pub-id></element-citation></ref><ref id="b4-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Bartel</surname><given-names>D.P.</given-names></name></person-group><year>2009</year><article-title>MicroRNAs: target recognition and regulatory functions</article-title><source>Cell</source><volume>136</volume><fpage>215</fpage><lpage>233</lpage><pub-id pub-id-type="pmid">19167326</pub-id></element-citation></ref><ref id="b5-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Borchert</surname><given-names>G.M.</given-names></name><name><surname>Holton</surname><given-names>N.W.</given-names></name><name><surname>Williams</surname><given-names>J.D.</given-names></name><name><surname>Hernan</surname><given-names>W.L.</given-names></name><name><surname>Bishop</surname><given-names>I.P.</given-names></name><name><surname>Dembosky</surname><given-names>J.A.</given-names></name><name><surname>Elste</surname><given-names>J.E.</given-names></name><name><surname>Gregoire</surname><given-names>N.S.</given-names></name><name><surname>Kim</surname><given-names>J.A.</given-names></name><name><surname>Koehler</surname><given-names>W.W.</given-names></name><etal></etal></person-group><year>2011</year><article-title>Comprehensive analysis of microRNA genomic loci identifies pervasive repetitive-element origins</article-title><source>Mob Genet Elements</source><volume>1</volume><fpage>8</fpage><lpage>17</lpage><pub-id pub-id-type="pmid">22016841</pub-id></element-citation></ref><ref id="b6-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>K.</given-names></name><name><surname>Rajewsky</surname><given-names>N.</given-names></name></person-group><year>2007</year><article-title>The evolution of gene regulation by transcription factors and microRNAs</article-title><source>Nat Rev Genet</source><volume>8</volume><fpage>93</fpage><lpage>103</lpage><pub-id pub-id-type="pmid">17230196</pub-id></element-citation></ref><ref id="b7-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Coffin</surname><given-names>J.M.</given-names></name></person-group><year>2004</year><article-title>Evolution of retroviruses: fossils in our DNA</article-title><source>Proc Am Philos Soc</source><volume>148</volume><fpage>264</fpage><lpage>280</lpage><pub-id pub-id-type="pmid">15551500</pub-id></element-citation></ref><ref id="b8-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Doan</surname><given-names>R.</given-names></name><name><surname>Cohen</surname><given-names>N.</given-names></name><name><surname>Harrington</surname><given-names>J.</given-names></name><name><surname>Veazey</surname><given-names>K.</given-names></name><name><surname>Juras</surname><given-names>R.</given-names></name><name><surname>Cothran</surname><given-names>G.</given-names></name><name><surname>McCue</surname><given-names>M.E.</given-names></name><name><surname>Skow</surname><given-names>L.</given-names></name><name><surname>Dindot</surname><given-names>S.V.</given-names></name></person-group><year>2012</year><article-title>Identification of copy number variants in horses</article-title><source>Genome Res</source><volume>22</volume><fpage>899</fpage><lpage>907</lpage><pub-id pub-id-type="pmid">22383489</pub-id></element-citation></ref><ref id="b9-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Fang</surname><given-names>R.</given-names></name><name><surname>Olds</surname><given-names>L.C.</given-names></name><name><surname>Sibley</surname><given-names>E.</given-names></name></person-group><year>2006</year><article-title>Spatio-temporal patterns of intestine-specific transcription factor expression during postnatal mouse gut development</article-title><source>Gene Expr Patterns</source><volume>6</volume><fpage>426</fpage><lpage>432</lpage><pub-id pub-id-type="pmid">16377257</pub-id></element-citation></ref><ref id="b10-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Feschotte</surname><given-names>C.</given-names></name><name><surname>Swamy</surname><given-names>L.</given-names></name><name><surname>Wessler</surname><given-names>S.R.</given-names></name></person-group><year>2003</year><article-title>Genome-wide analysis of mariner-like transposable elements in rice reveals complex relationships with stowaway miniature inverted repeat transposable elements (MITEs)</article-title><source>Genetics</source><volume>163</volume><fpage>747</fpage><lpage>758</lpage><pub-id pub-id-type="pmid">12618411</pub-id></element-citation></ref><ref id="b11-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Fu</surname><given-names>H.J.</given-names></name><name><surname>Zhu</surname><given-names>J.</given-names></name><name><surname>Yang</surname><given-names>M.</given-names></name><name><surname>Zhang</surname><given-names>Z.Y.</given-names></name><name><surname>Tie</surname><given-names>Y.</given-names></name><name><surname>Jiang</surname><given-names>H.</given-names></name><name><surname>Sun</surname><given-names>Z.X.</given-names></name><name><surname>Zheng</surname><given-names>X.F.</given-names></name></person-group><year>2006</year><article-title>A novel method to monitor the expression of microRNAs</article-title><source>Mol Biotechnol</source><volume>32</volume><fpage>197</fpage><lpage>204</lpage><pub-id pub-id-type="pmid">16632886</pub-id></element-citation></ref><ref id="b12-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gim</surname><given-names>J.-A.</given-names></name><name><surname>Ha</surname><given-names>H.-S.</given-names></name><name><surname>Ahn</surname><given-names>K.</given-names></name><name><surname>Kim</surname><given-names>D.-S.</given-names></name><name><surname>Kim</surname><given-names>H.-S.</given-names></name></person-group><year>2014</year><article-title>Genome-wide identification and classification of microRNAs derived from repetitive elements</article-title><source>Genomics Inform</source><volume>12</volume><fpage>261</fpage><lpage>267</lpage><pub-id pub-id-type="pmid">25705168</pub-id></element-citation></ref><ref id="b13-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gim</surname><given-names>J.A.</given-names></name><name><surname>Hong</surname><given-names>C.P.</given-names></name><name><surname>Kim</surname><given-names>D.S.</given-names></name><name><surname>Moon</surname><given-names>J.W.</given-names></name><name><surname>Choi</surname><given-names>Y.</given-names></name><name><surname>Eo</surname><given-names>J.</given-names></name><name><surname>Kwon</surname><given-names>Y.J.</given-names></name><name><surname>Lee</surname><given-names>J.R.</given-names></name><name><surname>Jung</surname><given-names>Y.D.</given-names></name><name><surname>Bae</surname><given-names>J.H.</given-names></name><etal></etal></person-group><year>2015</year><article-title>Genome-wide analysis of DNA methylation before-and after exercise in the thoroughbred horse with MeDIP-Seq</article-title><source>Mol Cells</source><volume>38</volume><fpage>210</fpage><lpage>220</lpage><pub-id pub-id-type="pmid">25666347</pub-id></element-citation></ref><ref id="b14-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hayashita</surname><given-names>Y.</given-names></name><name><surname>Osada</surname><given-names>H.</given-names></name><name><surname>Tatematsu</surname><given-names>Y.</given-names></name><name><surname>Yamada</surname><given-names>H.</given-names></name><name><surname>Yanagisawa</surname><given-names>K.</given-names></name><name><surname>Tomida</surname><given-names>S.</given-names></name><name><surname>Yatabe</surname><given-names>Y.</given-names></name><name><surname>Kawahara</surname><given-names>K.</given-names></name><name><surname>Sekido</surname><given-names>Y.</given-names></name><name><surname>Takahashi</surname><given-names>T.</given-names></name></person-group><year>2005</year><article-title>A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation</article-title><source>Cancer Res</source><volume>65</volume><fpage>9628</fpage><lpage>9632</lpage><pub-id pub-id-type="pmid">16266980</pub-id></element-citation></ref><ref id="b15-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hill</surname><given-names>E.W.</given-names></name><name><surname>Gu</surname><given-names>J.</given-names></name><name><surname>McGivney</surname><given-names>B.A.</given-names></name><name><surname>MacHugh</surname><given-names>D.E.</given-names></name></person-group><year>2010</year><article-title>Targets of selection in the Thoroughbred genome contain exercise-relevant gene SNPs associated with elite racecourse performance</article-title><source>Anim Genet</source><volume>41</volume><issue>Suppl 2</issue><fpage>56</fpage><lpage>63</lpage><pub-id pub-id-type="pmid">21070277</pub-id></element-citation></ref><ref id="b16-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hubbard</surname><given-names>T.</given-names></name><name><surname>Barker</surname><given-names>D.</given-names></name><name><surname>Birney</surname><given-names>E.</given-names></name><name><surname>Cameron</surname><given-names>G.</given-names></name><name><surname>Chen</surname><given-names>Y.</given-names></name><name><surname>Clark</surname><given-names>L.</given-names></name><name><surname>Cox</surname><given-names>T.</given-names></name><name><surname>Cuff</surname><given-names>J.</given-names></name><name><surname>Curwen</surname><given-names>V.</given-names></name><name><surname>Down</surname><given-names>T.</given-names></name></person-group><year>2002</year><article-title>The Ensembl genome database project</article-title><source>Nucleic Acids Res</source><volume>30</volume><fpage>38</fpage><lpage>41</lpage><pub-id pub-id-type="pmid">11752248</pub-id></element-citation></ref><ref id="b17-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hutvagner</surname><given-names>G.</given-names></name></person-group><year>2005</year><article-title>Small RNA asymmetry in RNAi: function in RISC assembly and gene regulation</article-title><source>FEBS Lett</source><volume>579</volume><fpage>5850</fpage><lpage>5857</lpage><pub-id pub-id-type="pmid">16199039</pub-id></element-citation></ref><ref id="b18-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jurka</surname><given-names>J.</given-names></name></person-group><year>2000</year><article-title>Repbase update: a database and an electronic journal of repetitive elements</article-title><source>Trends Genet</source><volume>16</volume><fpage>418</fpage><lpage>420</lpage><pub-id pub-id-type="pmid">10973072</pub-id></element-citation></ref><ref id="b19-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jurka</surname><given-names>J.</given-names></name><name><surname>Kapitonov</surname><given-names>V.V.</given-names></name><name><surname>Klonowski</surname><given-names>P.</given-names></name><name><surname>Walichiewicz</surname><given-names>J.</given-names></name><name><surname>Smit</surname><given-names>A.F.</given-names></name></person-group><year>1996</year><article-title>Identification of new medium reiteration frequency repeats in the genomes of Primates, Rodentia and Lagomorpha</article-title><source>Genetica</source><volume>98</volume><fpage>235</fpage><lpage>247</lpage><pub-id pub-id-type="pmid">9204548</pub-id></element-citation></ref><ref id="b20-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jurka</surname><given-names>J.</given-names></name><name><surname>Kapitonov</surname><given-names>V.V.</given-names></name><name><surname>Pavlicek</surname><given-names>A.</given-names></name><name><surname>Klonowski</surname><given-names>P.</given-names></name><name><surname>Kohany</surname><given-names>O.</given-names></name><name><surname>Walichiewicz</surname><given-names>J.</given-names></name></person-group><year>2005</year><article-title>Repbase Update, a database of eukaryotic repetitive elements</article-title><source>Cytogenet Genome Res</source><volume>110</volume><fpage>462</fpage><lpage>467</lpage><pub-id pub-id-type="pmid">16093699</pub-id></element-citation></ref><ref id="b21-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kim</surname><given-names>M.C.</given-names></name><name><surname>Lee</surname><given-names>S.W.</given-names></name><name><surname>Ryu</surname><given-names>D.Y.</given-names></name><name><surname>Cui</surname><given-names>F.J.</given-names></name><name><surname>Bhak</surname><given-names>J.</given-names></name><name><surname>Kim</surname><given-names>Y.</given-names></name></person-group><year>2014</year><article-title>Identification and characterization of microRNAs in normal equine tissues by next generation sequencing</article-title><source>PLoS One</source><volume>9</volume><fpage>e93662</fpage><pub-id pub-id-type="pmid">24695583</pub-id></element-citation></ref><ref id="b22-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kozomara</surname><given-names>A.</given-names></name><name><surname>Griffiths-Jones</surname><given-names>S.</given-names></name></person-group><year>2014</year><article-title>miRBase: annotating high confidence microRNAs using deep sequencing data</article-title><source>Nucleic Acids Res</source><volume>42</volume><fpage>D68</fpage><lpage>73</lpage><pub-id pub-id-type="pmid">24275495</pub-id></element-citation></ref><ref id="b23-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lamprecht</surname><given-names>B.</given-names></name><name><surname>Walter</surname><given-names>K.</given-names></name><name><surname>Kreher</surname><given-names>S.</given-names></name><name><surname>Kumar</surname><given-names>R.</given-names></name><name><surname>Hummel</surname><given-names>M.</given-names></name><name><surname>Lenze</surname><given-names>D.</given-names></name><name><surname>Kochert</surname><given-names>K.</given-names></name><name><surname>Bouhlel</surname><given-names>M.A.</given-names></name><name><surname>Richter</surname><given-names>J.</given-names></name><name><surname>Soler</surname><given-names>E.</given-names></name><etal></etal></person-group><year>2010</year><article-title>Derepression of an endogenous long terminal repeat activates the CSF1R proto-oncogene in human lymphoma</article-title><source>Nat Med</source><volume>16</volume><fpage>571</fpage><lpage>579</lpage><pub-id pub-id-type="pmid">20436485</pub-id></element-citation></ref><ref id="b24-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lander</surname><given-names>E.S.</given-names></name><name><surname>Linton</surname><given-names>L.M.</given-names></name><name><surname>Birren</surname><given-names>B.</given-names></name><name><surname>Nusbaum</surname><given-names>C.</given-names></name><name><surname>Zody</surname><given-names>M.C.</given-names></name><name><surname>Baldwin</surname><given-names>J.</given-names></name><name><surname>Devon</surname><given-names>K.</given-names></name><name><surname>Dewar</surname><given-names>K.</given-names></name><name><surname>Doyle</surname><given-names>M.</given-names></name><name><surname>FitzHugh</surname><given-names>W.</given-names></name><etal></etal></person-group><year>2001</year><article-title>Initial sequencing and analysis of the human genome</article-title><source>Nature</source><volume>409</volume><fpage>860</fpage><lpage>921</lpage><pub-id pub-id-type="pmid">11237011</pub-id></element-citation></ref><ref id="b25-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lee</surname><given-names>Y.</given-names></name><name><surname>Kim</surname><given-names>M.</given-names></name><name><surname>Han</surname><given-names>J.</given-names></name><name><surname>Yeom</surname><given-names>K.H.</given-names></name><name><surname>Lee</surname><given-names>S.</given-names></name><name><surname>Baek</surname><given-names>S.H.</given-names></name><name><surname>Kim</surname><given-names>V.N.</given-names></name></person-group><year>2004</year><article-title>MicroRNA genes are transcribed by RNA polymerase II</article-title><source>EMBO J</source><volume>23</volume><fpage>4051</fpage><lpage>4060</lpage><pub-id pub-id-type="pmid">15372072</pub-id></element-citation></ref><ref id="b26-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lein</surname><given-names>E.S.</given-names></name><name><surname>Hawrylycz</surname><given-names>M.J.</given-names></name><name><surname>Ao</surname><given-names>N.</given-names></name><name><surname>Ayres</surname><given-names>M.</given-names></name><name><surname>Bensinger</surname><given-names>A.</given-names></name><name><surname>Bernard</surname><given-names>A.</given-names></name><name><surname>Boe</surname><given-names>A.F.</given-names></name><name><surname>Boguski</surname><given-names>M.S.</given-names></name><name><surname>Brockway</surname><given-names>K.S.</given-names></name><name><surname>Byrnes</surname><given-names>E.J.</given-names></name></person-group><year>2007</year><article-title>Genome-wide atlas of gene expression in the adult mouse brain</article-title><source>Nature</source><volume>445</volume><fpage>168</fpage><lpage>176</lpage><pub-id pub-id-type="pmid">17151600</pub-id></element-citation></ref><ref id="b27-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Li</surname><given-names>Y.</given-names></name><name><surname>Xie</surname><given-names>J.</given-names></name><name><surname>Xu</surname><given-names>X.</given-names></name><name><surname>Wang</surname><given-names>J.</given-names></name><name><surname>Ao</surname><given-names>F.</given-names></name><name><surname>Wan</surname><given-names>Y.</given-names></name><name><surname>Zhu</surname><given-names>Y.</given-names></name></person-group><year>2013</year><article-title>MicroRNA-548 down-regulates host antiviral response via direct targeting of IFN-λ1</article-title><source>Protein Cell</source><volume>4</volume><fpage>130</fpage><lpage>141</lpage><pub-id pub-id-type="pmid">23150165</pub-id></element-citation></ref><ref id="b28-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Liang</surname><given-names>T.</given-names></name><name><surname>Guo</surname><given-names>L.</given-names></name><name><surname>Liu</surname><given-names>C.</given-names></name></person-group><year>2012</year><article-title>Genome-wide analysis of mir-548 gene family reveals evolutionary and functional implications</article-title><source>J Biomed Biotechnol</source><volume>2012</volume><fpage>679563</fpage><pub-id pub-id-type="pmid">23091353</pub-id></element-citation></ref><ref id="b29-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lund</surname><given-names>E.</given-names></name><name><surname>Dahlberg</surname><given-names>J.E.</given-names></name></person-group><year>2006</year><article-title>Substrate selectivity of exportin 5 and Dicer in the biogenesis of microRNAs</article-title><source>Cold Spring Harb Symp Quant Biol</source><volume>71</volume><fpage>59</fpage><lpage>66</lpage><pub-id pub-id-type="pmid">17381281</pub-id></element-citation></ref><ref id="b30-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nishimura</surname><given-names>M.</given-names></name><name><surname>Naito</surname><given-names>S.</given-names></name></person-group><year>2005</year><article-title>Tissue-specific mRNA expression profiles of human toll-like receptors and related genes</article-title><source>Biol Pharm Bull</source><volume>28</volume><fpage>886</fpage><lpage>892</lpage><pub-id pub-id-type="pmid">15863899</pub-id></element-citation></ref><ref id="b31-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nozawa</surname><given-names>M.</given-names></name><name><surname>Miura</surname><given-names>S.</given-names></name><name><surname>Nei</surname><given-names>M.</given-names></name></person-group><year>2010</year><article-title>Origins and evolution of microRNA genes in Drosophila species</article-title><source>Genome Biol Evol</source><volume>2</volume><fpage>180</fpage><lpage>189</lpage><pub-id pub-id-type="pmid">20624724</pub-id></element-citation></ref><ref id="b32-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ota</surname><given-names>A.</given-names></name><name><surname>Tagawa</surname><given-names>H.</given-names></name><name><surname>Karnan</surname><given-names>S.</given-names></name><name><surname>Tsuzuki</surname><given-names>S.</given-names></name><name><surname>Karpas</surname><given-names>A.</given-names></name><name><surname>Kira</surname><given-names>S.</given-names></name><name><surname>Yoshida</surname><given-names>Y.</given-names></name><name><surname>Seto</surname><given-names>M.</given-names></name></person-group><year>2004</year><article-title>Identification and characterization of a novel gene, C13orf25, as a target for 13q31–q32 amplification in malignant lymphoma</article-title><source>Cancer Res</source><volume>64</volume><fpage>3087</fpage><lpage>3095</lpage><pub-id pub-id-type="pmid">15126345</pub-id></element-citation></ref><ref id="b33-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ou-Yang</surname><given-names>F.</given-names></name><name><surname>Luo</surname><given-names>Q.-J.</given-names></name><name><surname>Zhang</surname><given-names>Y.</given-names></name><name><surname>Richardson</surname><given-names>C.R.</given-names></name><name><surname>Jiang</surname><given-names>Y.</given-names></name><name><surname>Rock</surname><given-names>C.D.</given-names></name></person-group><year>2013</year><article-title>Transposable element-associated microRNA hairpins produce 21-nt sRNAs integrated into typical microRNA pathways in rice</article-title><source>Funct Integr Genomics</source><volume>13</volume><fpage>207</fpage><lpage>216</lpage><pub-id pub-id-type="pmid">23420033</pub-id></element-citation></ref><ref id="b34-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pace</surname><given-names>J.K.</given-names></name>2nd<name><surname>Feschotte</surname><given-names>C.</given-names></name></person-group><year>2007</year><article-title>The evolutionary history of human DNA transposons: evidence for intense activity in the primate lineage</article-title><source>Genome Res</source><volume>17</volume><fpage>422</fpage><lpage>432</lpage><pub-id pub-id-type="pmid">17339369</pub-id></element-citation></ref><ref id="b35-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Park</surname><given-names>S.J.</given-names></name><name><surname>Kim</surname><given-names>Y.H.</given-names></name><name><surname>Lee</surname><given-names>S.R.</given-names></name><name><surname>Choe</surname><given-names>S.H.</given-names></name><name><surname>Kim</surname><given-names>M.J.</given-names></name><name><surname>Kim</surname><given-names>S.U.</given-names></name><name><surname>Kim</surname><given-names>J.S.</given-names></name><name><surname>Sim</surname><given-names>B.W.</given-names></name><name><surname>Song</surname><given-names>B.S.</given-names></name><name><surname>Jeong</surname><given-names>K.J.</given-names></name><etal></etal></person-group><year>2015</year><article-title>Gain of a new exon by a lineage-specific alu element-integration event in the BCS1L gene during primate evolution</article-title><source>Mol Cells</source><volume>38</volume><fpage>950</fpage><lpage>958</lpage><pub-id pub-id-type="pmid">26537194</pub-id></element-citation></ref><ref id="b36-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Petersen</surname><given-names>J.L.</given-names></name><name><surname>Mickelson</surname><given-names>J.R.</given-names></name><name><surname>Rendahl</surname><given-names>A.K.</given-names></name><name><surname>Valberg</surname><given-names>S.J.</given-names></name><name><surname>Andersson</surname><given-names>L.S.</given-names></name><name><surname>Axelsson</surname><given-names>J.</given-names></name><name><surname>Bailey</surname><given-names>E.</given-names></name><name><surname>Bannasch</surname><given-names>D.</given-names></name><name><surname>Binns</surname><given-names>M.M.</given-names></name><name><surname>Borges</surname><given-names>A.S.</given-names></name><etal></etal></person-group><year>2013</year><article-title>Genome-wide analysis reveals selection for important traits in domestic horse breeds</article-title><source>PLoS Genet</source><volume>9</volume><fpage>e1003211</fpage><pub-id pub-id-type="pmid">23349635</pub-id></element-citation></ref><ref id="b37-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Piriyapongsa</surname><given-names>J.</given-names></name><name><surname>Jordan</surname><given-names>I.K.</given-names></name></person-group><year>2007</year><article-title>A family of human microRNA genes from miniature inverted-repeat transposable elements</article-title><source>PLoS One</source><volume>2</volume><fpage>e203</fpage><pub-id pub-id-type="pmid">17301878</pub-id></element-citation></ref><ref id="b38-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Piriyapongsa</surname><given-names>J.</given-names></name><name><surname>Jordan</surname><given-names>I.K.</given-names></name></person-group><year>2008</year><article-title>Dual coding of siRNAs and miRNAs by plant transposable elements</article-title><source>RNA</source><volume>14</volume><fpage>814</fpage><lpage>821</lpage><pub-id pub-id-type="pmid">18367716</pub-id></element-citation></ref><ref id="b39-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Piriyapongsa</surname><given-names>J.</given-names></name><name><surname>Marino-Ramirez</surname><given-names>L.</given-names></name><name><surname>Jordan</surname><given-names>I.K.</given-names></name></person-group><year>2007</year><article-title>Origin and evolution of human microRNAs from transposable elements</article-title><source>Genetics</source><volume>176</volume><fpage>1323</fpage><lpage>1337</lpage><pub-id pub-id-type="pmid">17435244</pub-id></element-citation></ref><ref id="b40-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ruike</surname><given-names>Y.</given-names></name><name><surname>Ichimura</surname><given-names>A.</given-names></name><name><surname>Tsuchiya</surname><given-names>S.</given-names></name><name><surname>Shimizu</surname><given-names>K.</given-names></name><name><surname>Kunimoto</surname><given-names>R.</given-names></name><name><surname>Okuno</surname><given-names>Y.</given-names></name><name><surname>Tsujimoto</surname><given-names>G.</given-names></name></person-group><year>2008</year><article-title>Global correlation analysis for micro-RNA and mRNA expression profiles in human cell lines</article-title><source>J Hum Genet</source><volume>53</volume><fpage>515</fpage><lpage>523</lpage><pub-id pub-id-type="pmid">18465083</pub-id></element-citation></ref><ref id="b41-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Shukla</surname><given-names>G.C.</given-names></name><name><surname>Singh</surname><given-names>J.</given-names></name><name><surname>Barik</surname><given-names>S.</given-names></name></person-group><year>2011</year><article-title>MicroRNAs: processing, maturation, target recognition and regulatory functions</article-title><source>Mol Cell Pharmacol</source><volume>3</volume><fpage>83</fpage><lpage>92</lpage><pub-id pub-id-type="pmid">22468167</pub-id></element-citation></ref><ref id="b42-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Smalheiser</surname><given-names>N.R.</given-names></name></person-group><year>2003</year><article-title>EST analyses predict the existence of a population of chimeric microRNA precursor-mRNA transcripts expressed in normal human and mouse tissues</article-title><source>Genome Biol</source><volume>4</volume><fpage>403</fpage><pub-id pub-id-type="pmid">12844357</pub-id></element-citation></ref><ref id="b43-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Smalheiser</surname><given-names>N.R.</given-names></name><name><surname>Torvik</surname><given-names>V.I.</given-names></name></person-group><year>2005</year><article-title>Mammalian microRNAs derived from genomic repeats</article-title><source>Trends Genet</source><volume>21</volume><fpage>322</fpage><lpage>326</lpage><pub-id pub-id-type="pmid">15922829</pub-id></element-citation></ref><ref id="b44-molce-40-4-262"><element-citation publication-type="book"><person-group person-group-type="author"><name><surname>Smit</surname><given-names>A.</given-names></name><name><surname>Hubley</surname><given-names>R.</given-names></name><name><surname>Green</surname><given-names>P.</given-names></name></person-group><year>2004</year><source>RepeatMasker Open-3.0. 2004</source><publisher-loc>Seattle (WA)</publisher-loc><publisher-name>Institute for Systems Biology</publisher-name></element-citation></ref><ref id="b45-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Smit</surname><given-names>A.F.</given-names></name></person-group><year>1993</year><article-title>Identification of a new, abundant superfamily of mammalian LTR-transposons</article-title><source>Nucleic Acids Res</source><volume>21</volume><fpage>1863</fpage><lpage>1872</lpage><pub-id pub-id-type="pmid">8388099</pub-id></element-citation></ref><ref id="b46-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wade</surname><given-names>C.M.</given-names></name><name><surname>Giulotto</surname><given-names>E.</given-names></name><name><surname>Sigurdsson</surname><given-names>S.</given-names></name><name><surname>Zoli</surname><given-names>M.</given-names></name><name><surname>Gnerre</surname><given-names>S.</given-names></name><name><surname>Imsland</surname><given-names>F.</given-names></name><name><surname>Lear</surname><given-names>T.L.</given-names></name><name><surname>Adelson</surname><given-names>D.L.</given-names></name><name><surname>Bailey</surname><given-names>E.</given-names></name><name><surname>Bellone</surname><given-names>R.R.</given-names></name><etal></etal></person-group><year>2009</year><article-title>Genome sequence, comparative analysis, and population genetics of the domestic horse</article-title><source>Science</source><volume>326</volume><fpage>865</fpage><lpage>867</lpage><pub-id pub-id-type="pmid">19892987</pub-id></element-citation></ref><ref id="b47-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Yuan</surname><given-names>Z.</given-names></name><name><surname>Sun</surname><given-names>X.</given-names></name><name><surname>Jiang</surname><given-names>D.</given-names></name><name><surname>Ding</surname><given-names>Y.</given-names></name><name><surname>Lu</surname><given-names>Z.</given-names></name><name><surname>Gong</surname><given-names>L.</given-names></name><name><surname>Liu</surname><given-names>H.</given-names></name><name><surname>Xie</surname><given-names>J.</given-names></name></person-group><year>2010</year><article-title>Origin and evolution of a placental-specific microRNA family in the human genome</article-title><source>BMC Evol Biol</source><volume>10</volume><fpage>346</fpage><pub-id pub-id-type="pmid">21067568</pub-id></element-citation></ref><ref id="b48-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Yuan</surname><given-names>Z.</given-names></name><name><surname>Sun</surname><given-names>X.</given-names></name><name><surname>Liu</surname><given-names>H.</given-names></name><name><surname>Xie</surname><given-names>J.</given-names></name></person-group><year>2011</year><article-title>MicroRNA genes derived from repetitive elements and expanded by segmental duplication events in mammalian genomes</article-title><source>PLoS One</source><volume>6</volume><fpage>e17666</fpage><pub-id pub-id-type="pmid">21436881</pub-id></element-citation></ref><ref id="b49-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zhang</surname><given-names>Q.</given-names></name><name><surname>Arbuckle</surname><given-names>J.</given-names></name><name><surname>Wessler</surname><given-names>S.R.</given-names></name></person-group><year>2000</year><article-title>Recent, extensive, and preferential insertion of members of the miniature inverted-repeat transposable element family Heartbreaker into genic regions of maize</article-title><source>Proc Natl Acad Sci USA</source><volume>97</volume><fpage>1160</fpage><lpage>1165</lpage><pub-id pub-id-type="pmid">10655501</pub-id></element-citation></ref><ref id="b50-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zhang</surname><given-names>H.</given-names></name><name><surname>Liu</surname><given-names>Y.</given-names></name><name><surname>Su</surname><given-names>D.</given-names></name><name><surname>Yang</surname><given-names>Y.</given-names></name><name><surname>Bai</surname><given-names>G.</given-names></name><name><surname>Tao</surname><given-names>D.</given-names></name><name><surname>Ma</surname><given-names>Y.</given-names></name><name><surname>Zhang</surname><given-names>S.</given-names></name></person-group><year>2011</year><article-title>A single nucleotide polymorphism in a miR-1302 binding site in CGA increases the risk of idiopathic male infertility</article-title><source>Fertil Steril</source><volume>96</volume><fpage>34</fpage><lpage>39 e37</lpage><pub-id pub-id-type="pmid">21601192</pub-id></element-citation></ref><ref id="b51-molce-40-4-262"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zhou</surname><given-names>M.</given-names></name><name><surname>Wang</surname><given-names>Q.</given-names></name><name><surname>Sun</surname><given-names>J.</given-names></name><name><surname>Li</surname><given-names>X.</given-names></name><name><surname>Xu</surname><given-names>L.</given-names></name><name><surname>Yang</surname><given-names>H.</given-names></name><name><surname>Shi</surname><given-names>H.</given-names></name><name><surname>Ning</surname><given-names>S.</given-names></name><name><surname>Chen</surname><given-names>L.</given-names></name><name><surname>Li</surname><given-names>Y.</given-names></name><etal></etal></person-group><year>2009</year><article-title>In silico detection and characteristics of novel microRNA genes in the Equus caballus genome using an integrated ab initio and comparative genomic approach</article-title><source>Genomics</source><volume>94</volume><fpage>125</fpage><lpage>131</lpage><pub-id pub-id-type="pmid">19406225</pub-id></element-citation></ref></ref-list></back><floats-group><fig id="f1-molce-40-4-262" orientation="portrait" position="float"><label>Fig. 1</label><caption><p>MER consensus sequences matched with horse transcripts from Repbase version 21.05.</p><p>MER5A1 was identified in Eutheria. MER53, MER81, MER91C, and MER117 were identified in human.</p></caption><graphic xlink:href="molce-40-4-262f1"></graphic></fig><fig id="f2-molce-40-4-262" orientation="portrait" position="float"><label>Fig. 2</label><caption><p>Quantitative expression patterns of MER-derived transcript miRNAs.</p><p>(A) MER5A1-derived transcript ENSECAT00000029718 5p (left) and 3p (right) miRNA. (B) MER53-derived transcript ENSECAT00000027640 5p (left) and 3p (right) miRNA. (C) MER81-derived transcript ENSECAT00000029221 5p (left) and 3p (right) miRNA. (D) MER91C-derived transcript ENSECAT00000027964 5p (left) and 3p (right) miRNA. Each samples was examined in triplicate (Bar: mean; Whisker: standard deviation). Paired Student’s <italic>t</italic>-test was performed to verify statistical significance between the expression levels of two samples (*p < 0.05; **p < 0.01; ***p < 0.005). Two MER5A1-derived miRNAs were compared lung (A), MER91C-derived 5p miRNAs were compared duodenum (D, left), MER91C-derived 3p miRNAs were compared cerebrum (D, right), with other tissues.</p></caption><graphic xlink:href="molce-40-4-262f2"></graphic></fig><fig id="f3-molce-40-4-262" orientation="portrait" position="float"><label>Fig. 3</label><caption><p>Quantitative expression patterns of EST polycistronic miRNAs.</p><p>(A–E) Five mature miRNAs from horse EST. (F) Secondary structure prediction of BM734541.1, indicated are each mature sequence. Each samples was examined in triplicate (Bar: mean; Whisker: standard deviation). Paired Student’s <italic>t</italic>-test was performed to verify statistical significance between the expression levels of two samples (*p < 0.05). All miRNAs were compared cerebellum with other tissues.</p></caption><graphic xlink:href="molce-40-4-262f3"></graphic></fig><table-wrap id="t1-molce-40-4-262" orientation="portrait" position="float"><label>Table 1</label><caption><p>Palindromic MER consensus sequences in horse genome</p></caption><table frame="hsides" rules="groups"><thead><tr><th valign="middle" align="center" rowspan="1" colspan="1">Repeat name</th><th valign="middle" align="center" rowspan="1" colspan="1">Repeat length (bp)</th><th valign="middle" align="center" rowspan="1" colspan="1">Structure</th><th valign="middle" align="center" rowspan="1" colspan="1">Number of paralogs in horse genome (equCab2)</th></tr></thead><tbody><tr><td valign="top" align="center" rowspan="1" colspan="1">MER5A1</td><td valign="top" align="center" rowspan="1" colspan="1">160</td><td valign="top" align="center" rowspan="1" colspan="1">Palindrome</td><td valign="top" align="center" rowspan="1" colspan="1">196</td></tr><tr><td valign="top" align="center" rowspan="1" colspan="1">MER53</td><td valign="top" align="center" rowspan="1" colspan="1">189</td><td valign="top" align="center" rowspan="1" colspan="1">Palindrome</td><td valign="top" align="center" rowspan="1" colspan="1">202</td></tr><tr><td valign="top" align="center" rowspan="1" colspan="1">MER81</td><td valign="top" align="center" rowspan="1" colspan="1">114</td><td valign="top" align="center" rowspan="1" colspan="1">Palindrome</td><td valign="top" align="center" rowspan="1" colspan="1">161</td></tr><tr><td valign="top" align="center" rowspan="1" colspan="1">MER91C</td><td valign="top" align="center" rowspan="1" colspan="1">140</td><td valign="top" align="center" rowspan="1" colspan="1">Palindrome</td><td valign="top" align="center" rowspan="1" colspan="1">168</td></tr><tr><td valign="top" align="center" rowspan="1" colspan="1">MER117</td><td valign="top" align="center" rowspan="1" colspan="1">197</td><td valign="top" align="center" rowspan="1" colspan="1">Palindrome</td><td valign="top" align="center" rowspan="1" colspan="1">160</td></tr></tbody></table></table-wrap><table-wrap id="t2-molce-40-4-262" orientation="portrait" position="float"><label>Table 2</label><caption><p>Comparison between miRBase database sequences and palindromic MER-derived transcripts.</p><p>Matched precursor sequences and E-values were the results of the miRBase BLAST seaching.</p></caption><table frame="hsides" rules="groups"><thead><tr><th valign="top" align="left" rowspan="1" colspan="1">Repeat name</th><th valign="top" align="left" rowspan="1" colspan="1">Transcript assession no.</th><th valign="top" align="left" rowspan="1" colspan="1">Position</th><th valign="top" align="center" rowspan="1" colspan="1">Strand</th><th valign="top" align="center" rowspan="1" colspan="1">Transcript length (bp)</th><th valign="top" align="left" rowspan="1" colspan="1">Matched precursor sequence</th><th valign="top" align="left" rowspan="1" colspan="1">E-value</th><th valign="top" align="left" rowspan="1" colspan="1">Genomic region (Gene or transcript accession no.)</th></tr></thead><tbody><tr><td valign="top" align="left" rowspan="1" colspan="1">MER5A1</td><td valign="top" align="left" rowspan="1" colspan="1">ENSECAT00000029344.1</td><td valign="top" align="left" rowspan="1" colspan="1">chr14:89,097,198-89,097,294</td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1">97</td><td valign="top" align="left" rowspan="1" colspan="1">eca-miR-544b</td><td valign="top" align="left" rowspan="1" colspan="1">9.00E-15</td><td valign="top" align="left" rowspan="1" colspan="1">Intergenic</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"></td><td valign="top" align="left" rowspan="1" colspan="1">ENSECAT00000029411.1</td><td valign="top" align="left" rowspan="1" colspan="1">chr21:31,003,689-31,003,780</td><td valign="top" align="center" rowspan="1" colspan="1">−</td><td valign="top" align="center" rowspan="1" colspan="1">92</td><td valign="top" align="left" rowspan="1" colspan="1">eca-miR-544b</td><td valign="top" align="left" rowspan="1" colspan="1">6.00E-06</td><td valign="top" align="left" rowspan="1" colspan="1">Intron (ADAMTS12)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"></td><td valign="top" align="left" rowspan="1" colspan="1">ENSECAT00000029517.1</td><td valign="top" align="left" rowspan="1" colspan="1">chrX:10,032,472-10,032,568</td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1">97</td><td valign="top" align="left" rowspan="1" colspan="1">eca-miR-544b</td><td valign="top" align="left" rowspan="1" colspan="1">5.00E-14</td><td valign="top" align="left" rowspan="1" colspan="1">Intergenic</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"></td><td valign="top" align="left" rowspan="1" colspan="1">ENSECAT00000029551.1</td><td valign="top" align="left" rowspan="1" colspan="1">chr15:74,838,110-74,838,184</td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1">93</td><td valign="top" align="left" rowspan="1" colspan="1">eca-miR-544b</td><td valign="top" align="left" rowspan="1" colspan="1">1.00E-14</td><td valign="top" align="left" rowspan="1" colspan="1">Intergenic</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"></td><td valign="top" align="left" rowspan="1" colspan="1">ENSECAT00000029718.1</td><td valign="top" align="left" rowspan="1" colspan="1">chr11:53,066,395-53,066,487</td><td valign="top" align="center" rowspan="1" colspan="1">−</td><td valign="top" align="center" rowspan="1" colspan="1">93</td><td valign="top" align="left" rowspan="1" colspan="1">eca-miR-544b</td><td valign="top" align="left" rowspan="1" colspan="1">4.00E-08</td><td valign="top" align="left" rowspan="1" colspan="1">Intron (MYH1, MYH2, MYH4, MYH6, MYH7, MYH7B, MYH8, MYH13)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"></td><td valign="top" align="left" rowspan="1" colspan="1">ENSECAT00000029813.1</td><td valign="top" align="left" rowspan="1" colspan="1">chrX:19,077,964-19,078,058</td><td valign="top" align="center" rowspan="1" colspan="1">−</td><td valign="top" align="center" rowspan="1" colspan="1">95</td><td valign="top" align="left" rowspan="1" colspan="1">eca-miR-544b</td><td valign="top" align="left" rowspan="1" colspan="1">3.00E-09</td><td valign="top" align="left" rowspan="1" colspan="1">Intron (POLA)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">MER53</td><td valign="top" align="left" rowspan="1" colspan="1">ENSECAT00000027451.1</td><td valign="top" align="left" rowspan="1" colspan="1">chr16:58,884,211-58,884,360</td><td valign="top" align="center" rowspan="1" colspan="1">−</td><td valign="top" align="center" rowspan="1" colspan="1">150</td><td valign="top" align="left" rowspan="1" colspan="1">eca-miR-1302c-5</td><td valign="top" align="left" rowspan="1" colspan="1">1.00E-15</td><td valign="top" align="left" rowspan="1" colspan="1">Intron (JL635408)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"></td><td valign="top" align="left" rowspan="1" colspan="1">ENSECAT00000027640.1</td><td valign="top" align="left" rowspan="1" colspan="1">chr14:29,187,011-29,187,112</td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1">102</td><td valign="top" align="left" rowspan="1" colspan="1">eca-miR-1302-1</td><td valign="top" align="left" rowspan="1" colspan="1">6.00E-08</td><td valign="top" align="left" rowspan="1" colspan="1">Intron (HTR4, GU289397, AY647163)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">MER81</td><td valign="top" align="left" rowspan="1" colspan="1">ENSECAT00000029217.1</td><td valign="top" align="left" rowspan="1" colspan="1">chr10:36,160,777-36,160,851</td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1">75</td><td valign="top" align="left" rowspan="1" colspan="1">Not detected</td><td valign="top" align="left" rowspan="1" colspan="1"></td><td valign="top" align="left" rowspan="1" colspan="1">Intron (IBTK), UTR (JL626932)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"></td><td valign="top" align="left" rowspan="1" colspan="1">ENSECAT00000029221.1</td><td valign="top" align="left" rowspan="1" colspan="1">chrX:50,972,151-50,972,233</td><td valign="top" align="center" rowspan="1" colspan="1">−</td><td valign="top" align="center" rowspan="1" colspan="1">83</td><td valign="top" align="left" rowspan="1" colspan="1">Not detected</td><td valign="top" align="left" rowspan="1" colspan="1"></td><td valign="top" align="left" rowspan="1" colspan="1">Intergenic</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"></td><td valign="top" align="left" rowspan="1" colspan="1">ENSECAT00000029305.1</td><td valign="top" align="left" rowspan="1" colspan="1">chr21:8,851,113-8,851,188</td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1">76</td><td valign="top" align="left" rowspan="1" colspan="1">Not detected</td><td valign="top" align="left" rowspan="1" colspan="1"></td><td valign="top" align="left" rowspan="1" colspan="1">Intron (JL635478, JL639598, JL624325)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"></td><td valign="top" align="left" rowspan="1" colspan="1">ENSECAT00000029634.1</td><td valign="top" align="left" rowspan="1" colspan="1">chr2:37,821,443-37,821,510</td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1">68</td><td valign="top" align="left" rowspan="1" colspan="1">eca-miR-8990</td><td valign="top" align="left" rowspan="1" colspan="1">Not significant</td><td valign="top" align="left" rowspan="1" colspan="1">Intergenic</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">MER91C</td><td valign="top" align="left" rowspan="1" colspan="1">ENSECAT00000027964.1</td><td valign="top" align="left" rowspan="1" colspan="1">chrX:86,921,316-86,921,413</td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1">98</td><td valign="top" align="left" rowspan="1" colspan="1">eca-miR-652</td><td valign="top" align="left" rowspan="1" colspan="1">2.00E-34</td><td valign="top" align="left" rowspan="1" colspan="1">Intergenic</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">MER117</td><td valign="top" align="left" rowspan="1" colspan="1">BM734541.1</td><td valign="top" align="left" rowspan="1" colspan="1">chr23:7,068,821-7,073,667</td><td valign="top" align="center" rowspan="1" colspan="1">−</td><td valign="top" align="center" rowspan="1" colspan="1">655</td><td valign="top" align="left" rowspan="1" colspan="1">Not detected</td><td valign="top" align="left" rowspan="1" colspan="1"></td><td valign="top" align="left" rowspan="1" colspan="1">Intron (JL633484, JL641603)</td></tr></tbody></table></table-wrap></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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