Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus KOR/KNIH/002_05_2015, Isolated in South Korea
Identifieur interne : 000694 ( Pmc/Corpus ); précédent : 000693; suivant : 000695Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus KOR/KNIH/002_05_2015, Isolated in South Korea
Auteurs : You-Jin Kim ; Yong-Joon Cho ; Dae-Won Kim ; Jeong-Sun Yang ; Hak Kim ; Sunghan Park ; Young Woo Han ; Mi-Ran Yun ; Han Saem Lee ; A-Reum Kim ; Deok Rim Heo ; Joo Ae Kim ; Su Jin Kim ; Hee-Dong Jung ; Namil Kim ; Seok-Hwan Yoon ; Jeong-Gu Nam ; Hae Ji Kang ; Hyang-Min Cheong ; Joo-Shil Lee ; Jongsik Chun ; Sung Soon KimSource :
- Genome Announcements [ 2169-8287 ] ; 2015.
Abstract
The full genome sequence of a Middle East respiratory syndrome coronavirus (MERS-CoV) was identified from cultured and isolated in Vero cells. The viral genome sequence has high similarity to 53 human MERS-CoVs, ranging from 99.5% to 99.8% at the nucleotide level.
Url:
DOI: 10.1128/genomeA.00787-15
PubMed: 26272558
PubMed Central: 4536669
Links to Exploration step
PMC:4536669Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus KOR/KNIH/002_05_2015, Isolated in South Korea</title><author><name sortKey="Kim, You Jin" sort="Kim, You Jin" uniqKey="Kim Y" first="You-Jin" last="Kim">You-Jin Kim</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Cho, Yong Joon" sort="Cho, Yong Joon" uniqKey="Cho Y" first="Yong-Joon" last="Cho">Yong-Joon Cho</name><affiliation><nlm:aff id="aff2"><addr-line>ChunLab, Inc., Seoul National University, Seoul, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Dae Won" sort="Kim, Dae Won" uniqKey="Kim D" first="Dae-Won" last="Kim">Dae-Won Kim</name><affiliation><nlm:aff id="aff3"><addr-line>Division of Biosafety Evaluation and Control, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Yang, Jeong Sun" sort="Yang, Jeong Sun" uniqKey="Yang J" first="Jeong-Sun" last="Yang">Jeong-Sun Yang</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Hak" sort="Kim, Hak" uniqKey="Kim H" first="Hak" last="Kim">Hak Kim</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Park, Sunghan" sort="Park, Sunghan" uniqKey="Park S" first="Sunghan" last="Park">Sunghan Park</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Han, Young Woo" sort="Han, Young Woo" uniqKey="Han Y" first="Young Woo" last="Han">Young Woo Han</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Yun, Mi Ran" sort="Yun, Mi Ran" uniqKey="Yun M" first="Mi-Ran" last="Yun">Mi-Ran Yun</name><affiliation><nlm:aff id="aff3"><addr-line>Division of Biosafety Evaluation and Control, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Lee, Han Saem" sort="Lee, Han Saem" uniqKey="Lee H" first="Han Saem" last="Lee">Han Saem Lee</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, A Reum" sort="Kim, A Reum" uniqKey="Kim A" first="A-Reum" last="Kim">A-Reum Kim</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Heo, Deok Rim" sort="Heo, Deok Rim" uniqKey="Heo D" first="Deok Rim" last="Heo">Deok Rim Heo</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Joo Ae" sort="Kim, Joo Ae" uniqKey="Kim J" first="Joo Ae" last="Kim">Joo Ae Kim</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Su Jin" sort="Kim, Su Jin" uniqKey="Kim S" first="Su Jin" last="Kim">Su Jin Kim</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Jung, Hee Dong" sort="Jung, Hee Dong" uniqKey="Jung H" first="Hee-Dong" last="Jung">Hee-Dong Jung</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Namil" sort="Kim, Namil" uniqKey="Kim N" first="Namil" last="Kim">Namil Kim</name><affiliation><nlm:aff id="aff2"><addr-line>ChunLab, Inc., Seoul National University, Seoul, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Yoon, Seok Hwan" sort="Yoon, Seok Hwan" uniqKey="Yoon S" first="Seok-Hwan" last="Yoon">Seok-Hwan Yoon</name><affiliation><nlm:aff id="aff2"><addr-line>ChunLab, Inc., Seoul National University, Seoul, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Nam, Jeong Gu" sort="Nam, Jeong Gu" uniqKey="Nam J" first="Jeong-Gu" last="Nam">Jeong-Gu Nam</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kang, Hae Ji" sort="Kang, Hae Ji" uniqKey="Kang H" first="Hae Ji" last="Kang">Hae Ji Kang</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Cheong, Hyang Min" sort="Cheong, Hyang Min" uniqKey="Cheong H" first="Hyang-Min" last="Cheong">Hyang-Min Cheong</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Lee, Joo Shil" sort="Lee, Joo Shil" uniqKey="Lee J" first="Joo-Shil" last="Lee">Joo-Shil Lee</name><affiliation><nlm:aff id="aff4"><addr-line>Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chun, Jongsik" sort="Chun, Jongsik" uniqKey="Chun J" first="Jongsik" last="Chun">Jongsik Chun</name><affiliation><nlm:aff id="aff2"><addr-line>ChunLab, Inc., Seoul National University, Seoul, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Sung Soon" sort="Kim, Sung Soon" uniqKey="Kim S" first="Sung Soon" last="Kim">Sung Soon Kim</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26272558</idno><idno type="pmc">4536669</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4536669</idno><idno type="RBID">PMC:4536669</idno><idno type="doi">10.1128/genomeA.00787-15</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000694</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000694</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus KOR/KNIH/002_05_2015, Isolated in South Korea</title><author><name sortKey="Kim, You Jin" sort="Kim, You Jin" uniqKey="Kim Y" first="You-Jin" last="Kim">You-Jin Kim</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Cho, Yong Joon" sort="Cho, Yong Joon" uniqKey="Cho Y" first="Yong-Joon" last="Cho">Yong-Joon Cho</name><affiliation><nlm:aff id="aff2"><addr-line>ChunLab, Inc., Seoul National University, Seoul, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Dae Won" sort="Kim, Dae Won" uniqKey="Kim D" first="Dae-Won" last="Kim">Dae-Won Kim</name><affiliation><nlm:aff id="aff3"><addr-line>Division of Biosafety Evaluation and Control, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Yang, Jeong Sun" sort="Yang, Jeong Sun" uniqKey="Yang J" first="Jeong-Sun" last="Yang">Jeong-Sun Yang</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Hak" sort="Kim, Hak" uniqKey="Kim H" first="Hak" last="Kim">Hak Kim</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Park, Sunghan" sort="Park, Sunghan" uniqKey="Park S" first="Sunghan" last="Park">Sunghan Park</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Han, Young Woo" sort="Han, Young Woo" uniqKey="Han Y" first="Young Woo" last="Han">Young Woo Han</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Yun, Mi Ran" sort="Yun, Mi Ran" uniqKey="Yun M" first="Mi-Ran" last="Yun">Mi-Ran Yun</name><affiliation><nlm:aff id="aff3"><addr-line>Division of Biosafety Evaluation and Control, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Lee, Han Saem" sort="Lee, Han Saem" uniqKey="Lee H" first="Han Saem" last="Lee">Han Saem Lee</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, A Reum" sort="Kim, A Reum" uniqKey="Kim A" first="A-Reum" last="Kim">A-Reum Kim</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Heo, Deok Rim" sort="Heo, Deok Rim" uniqKey="Heo D" first="Deok Rim" last="Heo">Deok Rim Heo</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Joo Ae" sort="Kim, Joo Ae" uniqKey="Kim J" first="Joo Ae" last="Kim">Joo Ae Kim</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Su Jin" sort="Kim, Su Jin" uniqKey="Kim S" first="Su Jin" last="Kim">Su Jin Kim</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Jung, Hee Dong" sort="Jung, Hee Dong" uniqKey="Jung H" first="Hee-Dong" last="Jung">Hee-Dong Jung</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Namil" sort="Kim, Namil" uniqKey="Kim N" first="Namil" last="Kim">Namil Kim</name><affiliation><nlm:aff id="aff2"><addr-line>ChunLab, Inc., Seoul National University, Seoul, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Yoon, Seok Hwan" sort="Yoon, Seok Hwan" uniqKey="Yoon S" first="Seok-Hwan" last="Yoon">Seok-Hwan Yoon</name><affiliation><nlm:aff id="aff2"><addr-line>ChunLab, Inc., Seoul National University, Seoul, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Nam, Jeong Gu" sort="Nam, Jeong Gu" uniqKey="Nam J" first="Jeong-Gu" last="Nam">Jeong-Gu Nam</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kang, Hae Ji" sort="Kang, Hae Ji" uniqKey="Kang H" first="Hae Ji" last="Kang">Hae Ji Kang</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Cheong, Hyang Min" sort="Cheong, Hyang Min" uniqKey="Cheong H" first="Hyang-Min" last="Cheong">Hyang-Min Cheong</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Lee, Joo Shil" sort="Lee, Joo Shil" uniqKey="Lee J" first="Joo-Shil" last="Lee">Joo-Shil Lee</name><affiliation><nlm:aff id="aff4"><addr-line>Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chun, Jongsik" sort="Chun, Jongsik" uniqKey="Chun J" first="Jongsik" last="Chun">Jongsik Chun</name><affiliation><nlm:aff id="aff2"><addr-line>ChunLab, Inc., Seoul National University, Seoul, South Korea</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Sung Soon" sort="Kim, Sung Soon" uniqKey="Kim S" first="Sung Soon" last="Kim">Sung Soon Kim</name><affiliation><nlm:aff id="aff1"><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">Genome Announcements</title><idno type="eISSN">2169-8287</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The full genome sequence of a Middle East respiratory syndrome coronavirus (MERS-CoV) was identified from cultured and isolated in Vero cells. The viral genome sequence has high similarity to 53 human MERS-CoVs, ranging from 99.5% to 99.8% at the nucleotide level.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Coleman, Cm" uniqKey="Coleman C">CM Coleman</name></author><author><name sortKey="Frieman, Mb" uniqKey="Frieman M">MB Frieman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mackay, Im" uniqKey="Mackay I">IM Mackay</name></author><author><name sortKey="Arden, Ke" uniqKey="Arden K">KE Arden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cotten, M" uniqKey="Cotten M">M Cotten</name></author><author><name sortKey="Lam, Tt" uniqKey="Lam T">TT Lam</name></author><author><name sortKey="Watson, Sj" uniqKey="Watson S">SJ Watson</name></author><author><name sortKey="Palser, Al" uniqKey="Palser A">AL Palser</name></author><author><name sortKey="Petrova, V" uniqKey="Petrova V">V Petrova</name></author><author><name sortKey="Grant, P" uniqKey="Grant P">P Grant</name></author><author><name sortKey="Pybus, Og" uniqKey="Pybus O">OG Pybus</name></author><author><name sortKey="Rambaut, A" uniqKey="Rambaut A">A Rambaut</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Pillay, D" uniqKey="Pillay D">D Pillay</name></author><author><name sortKey="Kellam, P" uniqKey="Kellam P">P Kellam</name></author><author><name sortKey="Nastouli, E" uniqKey="Nastouli E">E Nastouli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Langmead, B" uniqKey="Langmead B">B Langmead</name></author><author><name sortKey="Salzberg, Sl" uniqKey="Salzberg S">SL Salzberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tamura, K" uniqKey="Tamura K">K Tamura</name></author><author><name sortKey="Stecher, G" uniqKey="Stecher G">G Stecher</name></author><author><name sortKey="Peterson, D" uniqKey="Peterson D">D Peterson</name></author><author><name sortKey="Filipski, A" uniqKey="Filipski A">A Filipski</name></author><author><name sortKey="Kumar, S" uniqKey="Kumar S">S Kumar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author><author><name sortKey="Shi, X" uniqKey="Shi X">X Shi</name></author><author><name sortKey="Jiang, L" uniqKey="Jiang L">L Jiang</name></author><author><name sortKey="Zhang, S" uniqKey="Zhang S">S Zhang</name></author><author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name></author><author><name sortKey="Tong, P" uniqKey="Tong P">P Tong</name></author><author><name sortKey="Guo, D" uniqKey="Guo D">D Guo</name></author><author><name sortKey="Fu, L" uniqKey="Fu L">L Fu</name></author><author><name sortKey="Cui, Y" uniqKey="Cui Y">Y Cui</name></author><author><name sortKey="Liu, X" uniqKey="Liu X">X Liu</name></author><author><name sortKey="Arledge, Kc" uniqKey="Arledge K">KC Arledge</name></author><author><name sortKey="Chen, Yh" uniqKey="Chen Y">YH Chen</name></author><author><name sortKey="Zhang, L" uniqKey="Zhang L">L Zhang</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="brief-report"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Genome Announc</journal-id><journal-id journal-id-type="iso-abbrev">Genome Announc</journal-id><journal-id journal-id-type="hwp">ga</journal-id><journal-id journal-id-type="pmc">ga</journal-id><journal-id journal-id-type="publisher-id">GA</journal-id><journal-title-group><journal-title>Genome Announcements</journal-title></journal-title-group><issn pub-type="epub">2169-8287</issn><publisher><publisher-name>American Society for Microbiology</publisher-name><publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26272558</article-id><article-id pub-id-type="pmc">4536669</article-id><article-id pub-id-type="publisher-id">genomeA00787-15</article-id><article-id pub-id-type="doi">10.1128/genomeA.00787-15</article-id><article-categories><subj-group subj-group-type="heading"><subject>Viruses</subject></subj-group></article-categories><title-group><article-title>Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus KOR/KNIH/002_05_2015, Isolated in South Korea</article-title><alt-title alt-title-type="running-head">Genome Announcement</alt-title><alt-title alt-title-type="short-authors">Kim et al.</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Kim</surname><given-names>You-Jin</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Cho</surname><given-names>Yong-Joon</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Dae-Won</given-names></name><xref ref-type="aff" rid="aff3"><sup>c</sup></xref></contrib><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Jeong-Sun</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Hak</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Park</surname><given-names>SungHan</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Han</surname><given-names>Young Woo</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Yun</surname><given-names>Mi-ran</given-names></name><xref ref-type="aff" rid="aff3"><sup>c</sup></xref></contrib><contrib contrib-type="author"><name><surname>Lee</surname><given-names>Han Saem</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>A-Reum</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Heo</surname><given-names>Deok Rim</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Joo Ae</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Su Jin</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Jung</surname><given-names>Hee-Dong</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Namil</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Yoon</surname><given-names>Seok-Hwan</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Nam</surname><given-names>Jeong-Gu</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kang</surname><given-names>Hae Ji</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Cheong</surname><given-names>Hyang-Min</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Lee</surname><given-names>Joo-Shil</given-names></name><xref ref-type="aff" rid="aff4"><sup>d</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Chun</surname><given-names>Jongsik</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Kim</surname><given-names>Sung Soon</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><aff id="aff1"><label>a</label><addr-line>Division of Respiratory Viruses, Center for Infectious Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheonju-si, South Korea</addr-line></aff><aff id="aff2"><label>b</label><addr-line>ChunLab, Inc., Seoul National University, Seoul, South Korea</addr-line></aff><aff id="aff3"><label>c</label><addr-line>Division of Biosafety Evaluation and Control, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, South Korea</addr-line></aff><aff id="aff4"><label>d</label><addr-line>Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju-si, South Korea</addr-line></aff></contrib-group><author-notes><corresp id="cor1">Address correspondence to Sung Soon Kim, <email>sungskim63@gmail.com</email>, or Jongsik Chun, <email>jchun@chunlab.com</email>.</corresp></author-notes><pub-date pub-type="epub"><day>13</day><month>8</month><year>2015</year></pub-date><pub-date pub-type="collection"><season>Jul-Aug</season><year>2015</year></pub-date><volume>3</volume><issue>4</issue><elocation-id>e00787-15</elocation-id><history><date date-type="received"><day>22</day><month>6</month><year>2015</year></date><date date-type="accepted"><day>22</day><month>6</month><year>2015</year></date></history><permissions><copyright-statement>Copyright © 2015 Kim et al.</copyright-statement><copyright-year>2015</copyright-year><copyright-holder>Kim et al.</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/3.0/"><license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/3.0/">Creative Commons Attribution 3.0 Unported license</ext-link>.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="jga004152774001.pdf"></self-uri><abstract><p>The full genome sequence of a Middle East respiratory syndrome coronavirus (MERS-CoV) was identified from cultured and isolated in Vero cells. The viral genome sequence has high similarity to 53 human MERS-CoVs, ranging from 99.5% to 99.8% at the nucleotide level.</p></abstract><counts><fig-count count="0"></fig-count><table-count count="0"></table-count><equation-count count="0"></equation-count><ref-count count="6"></ref-count><page-count count="2"></page-count><word-count count="994"></word-count></counts><custom-meta-group><custom-meta><meta-name>cover-date</meta-name><meta-value>July/August 2015</meta-value></custom-meta></custom-meta-group></article-meta></front><body><sec id="h0.0"><title>GENOME ANNOUNCEMENT</title><p>Middle East respiratory syndrome coronavirus (MERS-CoV) is the first betacoronavirus lineage C member isolated from humans. It has been assumed that MERS-CoV was transmitted from bats and spread to humans through intermediate hosts (<xref rid="B1" ref-type="bibr">1</xref>). The genome structure is a single-stranded RNA (ssRNA) encoding 10 proteins; two replicase polyproteins (open reading frames [ORFs] 1ab and 1a), three structural proteins (E, N, and M), a surface (spike) glycoprotein (S), and five nonstructural proteins (ORFs 3, 4a, 4b, and 5) (<xref rid="B2" ref-type="bibr">2</xref>).</p><p>A sputum sample was collected from a second patient on 20 May 2015. The MERS-CoV was inoculated to Vero cells and passed three times. The RNA was isolated from the third viral culture solution with the QIAamp viral RNA mini kit (QIAGEN, Germany). Reverse transcription was performed with the Superscript III first-strand synthesis system (Life Technologies, the Netherlands) with specific-reverse primers. The cDNA was amplified by overlapping PCR primers based on a previous study (<xref rid="B3" ref-type="bibr">3</xref>). Additional PCR primers were designed for nonamplified regions. The resulting PCR amplicons were pooled and fragmented to an average 300-bp length, and the sequencing library was constructed with an Illumina TruSeq Nano DNA sample prep kit (Illumina, USA). The sequencing was performed with an Illumina MiSeq 50-bp single-end platform (Illumina). A total of 2,814,805 sequence reads were generated, and 2,617,936 reads (93.01%) were mapped to the consensus sequence from human-origin MERS-CoV genome sequences retrieved from GenBank. Mapping was accomplished by Bowtie version 2.2.4 (<xref rid="B4" ref-type="bibr">4</xref>) with default parameters. Finally, the whole viral genome sequence was obtained from the mapped result with an average coverage of 3,605.95×. Based on the assembly, the genome size was estimated to be 30,108 bp with a GC content of 41.15%.</p><p>The sequence analysis of South Korean MERS-CoV was performed with 53 complete genomes of human MERS-CoV available from GenBank using MUSCLE in the MEGA version 6 package (<xref rid="B5" ref-type="bibr">5</xref>). The full-genome sequence of MERS-CoV/KOR/KNIH/002_05_2015 showed overall nucleotide identities of 99.5% to 99.8% with 53 human MERS-CoVs. The overall identity to EMC/2012 (accession no. JX869059), the reference genome, was 99.5%. The closest strain was Hafr-Al-Batin_1 (accession no. KF600628) with 99.8% similarity.</p><p>In this analysis, the Korean MERS-CoV includes 29 nucleotides and 12 amino acid variants, compared to 53 full-genome sequences for human MERS-CoV. Two specific variations, Arg137Ser in the N-terminal domain and Leu530Val in the receptor-binding domain, whose spike proteins mediate virus entry and affect the viral host range, were identified only in the cell-cultured MERS-CoV/KOR/KNIH/002_05_2015 (compare with other variation studies of the receptor-binding domain of the spike protein [<xref rid="B6" ref-type="bibr">6</xref>]).</p><sec id="s1"><title>Nucleotide sequence accession number.</title><p>The complete genome sequence of the MERS-CoV/KOR/KNIH/002_05_2015 isolate was deposited in GenBank under the accession number <ext-link ext-link-type="NCBI:nucleotide" xlink:href="KT029139">KT029139</ext-link>.</p></sec></sec></body><back><fn-group><fn fn-type="other"><p><bold>Citation</bold> Kim Y-J, Cho Y-J, Kim D-W, Yang J-S, Kim H, Park SH, Han YW, Yun M-R, Lee HS, Kim A-R, Heo DR, Kim JA, Kim SJ, Jung H-D, Kim N, Yoon S-H, Nam J-G, Kang HJ, Cheong H-M, Lee J-S, Chun J, Kim SS. 2015. Complete genome sequence of Middle East respiratory syndrome coronavirus KOR/KNIH/002_05_2015, isolated in South Korea. Genome Announc 3(4):e00787-15. doi:10.1128/genomeA.00787-15.</p></fn></fn-group><ack><title>ACKNOWLEDGMENTS</title><p>This work was supported by grants from the Intramural Research Fund (no. 2014-N47001-00) of the Korea National Institute of Health and the Intramural Fund (no. 4834-300-210-13) of the Korea Centers for Disease Control and Prevention.</p></ack><ref-list><title>REFERENCES</title><ref id="B1"><label>1.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name name-style="western"><surname>Coleman</surname><given-names>CM</given-names></name>, <name name-style="western"><surname>Frieman</surname><given-names>MB</given-names></name></person-group><year>2014</year><article-title>Coronaviruses: important emerging human pathogens</article-title>. <source>J Virol</source><volume>88</volume>:<fpage>5209</fpage>–<lpage>5212</lpage>. doi:<pub-id pub-id-type="doi">10.1128/JVI.03488-13</pub-id>.<pub-id pub-id-type="pmid">24600003</pub-id></mixed-citation></ref><ref id="B2"><label>2.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name name-style="western"><surname>Mackay</surname><given-names>IM</given-names></name>, <name name-style="western"><surname>Arden</surname><given-names>KE</given-names></name></person-group><year>2015</year><article-title>Middle East respiratory syndrome: an emerging coronavirus infection tracked by the crowd</article-title>. <source>Virus Res</source><volume>202</volume>:<fpage>60</fpage>–<lpage>88</lpage>. doi:<pub-id pub-id-type="doi">10.1016/j.virusres.2015.01.021</pub-id>.<pub-id pub-id-type="pmid">25656066</pub-id></mixed-citation></ref><ref id="B3"><label>3.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name name-style="western"><surname>Cotten</surname><given-names>M</given-names></name>, <name name-style="western"><surname>Lam</surname><given-names>TT</given-names></name>, <name name-style="western"><surname>Watson</surname><given-names>SJ</given-names></name>, <name name-style="western"><surname>Palser</surname><given-names>AL</given-names></name>, <name name-style="western"><surname>Petrova</surname><given-names>V</given-names></name>, <name name-style="western"><surname>Grant</surname><given-names>P</given-names></name>, <name name-style="western"><surname>Pybus</surname><given-names>OG</given-names></name>, <name name-style="western"><surname>Rambaut</surname><given-names>A</given-names></name>, <name name-style="western"><surname>Guan</surname><given-names>Y</given-names></name>, <name name-style="western"><surname>Pillay</surname><given-names>D</given-names></name>, <name name-style="western"><surname>Kellam</surname><given-names>P</given-names></name>, <name name-style="western"><surname>Nastouli</surname><given-names>E</given-names></name></person-group><year>2013</year><article-title>Full-genome deep sequencing and phylogenetic analysis of novel human betacoronavirus</article-title>. <source>Emerg Infect Dis</source><volume>19</volume>:<fpage>736B</fpage>–<lpage>742B</lpage>. doi:<pub-id pub-id-type="doi">10.3201/eid1905.130057</pub-id>.<pub-id pub-id-type="pmid">23693015</pub-id></mixed-citation></ref><ref id="B4"><label>4.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name name-style="western"><surname>Langmead</surname><given-names>B</given-names></name>, <name name-style="western"><surname>Salzberg</surname><given-names>SL</given-names></name></person-group><year>2012</year><article-title>Fast gapped-read alignment with Bowtie 2</article-title>. <source>Nat Methods</source><volume>9</volume>:<fpage>357</fpage>–<lpage>359</lpage>. doi:<pub-id pub-id-type="doi">10.1038/nmeth.1923</pub-id>.<pub-id pub-id-type="pmid">22388286</pub-id></mixed-citation></ref><ref id="B5"><label>5.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name name-style="western"><surname>Tamura</surname><given-names>K</given-names></name>, <name name-style="western"><surname>Stecher</surname><given-names>G</given-names></name>, <name name-style="western"><surname>Peterson</surname><given-names>D</given-names></name>, <name name-style="western"><surname>Filipski</surname><given-names>A</given-names></name>, <name name-style="western"><surname>Kumar</surname><given-names>S</given-names></name></person-group><year>2013</year><article-title>MEGA6: molecular evolutionary genetics analysis version 6.0</article-title>. <source>Mol Biol Evol</source><volume>30</volume>:<fpage>2725</fpage>–<lpage>2729</lpage>. doi:<pub-id pub-id-type="doi">10.1093/molbev/mst197</pub-id>.<pub-id pub-id-type="pmid">24132122</pub-id></mixed-citation></ref><ref id="B6"><label>6.</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><name name-style="western"><surname>Wang</surname><given-names>N</given-names></name>, <name name-style="western"><surname>Shi</surname><given-names>X</given-names></name>, <name name-style="western"><surname>Jiang</surname><given-names>L</given-names></name>, <name name-style="western"><surname>Zhang</surname><given-names>S</given-names></name>, <name name-style="western"><surname>Wang</surname><given-names>D</given-names></name>, <name name-style="western"><surname>Tong</surname><given-names>P</given-names></name>, <name name-style="western"><surname>Guo</surname><given-names>D</given-names></name>, <name name-style="western"><surname>Fu</surname><given-names>L</given-names></name>, <name name-style="western"><surname>Cui</surname><given-names>Y</given-names></name>, <name name-style="western"><surname>Liu</surname><given-names>X</given-names></name>, <name name-style="western"><surname>Arledge</surname><given-names>KC</given-names></name>, <name name-style="western"><surname>Chen</surname><given-names>YH</given-names></name>, <name name-style="western"><surname>Zhang</surname><given-names>L</given-names></name>, <name name-style="western"><surname>Wang</surname><given-names>X</given-names></name></person-group><year>2013</year><article-title>Structure of MERS-CoV spike receptor-binding domain complexed with human receptor DPP4</article-title>. <source>Cell Res</source><volume>23</volume>:<fpage>986</fpage>–<lpage>993</lpage>. doi:<pub-id pub-id-type="doi">10.1038/cr.2013.92</pub-id>.<pub-id pub-id-type="pmid">23835475</pub-id></mixed-citation></ref></ref-list></back></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000694 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000694 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé= PMC:4536669
|texte= Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus KOR/KNIH/002_05_2015, Isolated in South Korea
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:26272558" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |