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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development and validation of different indirect ELISAs for MERS-CoV serological testing</title><author><name sortKey="Al Amri, S" sort="Al Amri, S" uniqKey="Al Amri S" first="S." last="Al-Amri">S. Al-Amri</name><affiliation><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University</nlm:aff></affiliation></author><author><name sortKey="Al Subhi, T" sort="Al Subhi, T" uniqKey="Al Subhi T" first="T." last="Al-Subhi">T. Al-Subhi</name><affiliation><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University</nlm:aff></affiliation></author><author><name sortKey="Hassan, A" sort="Hassan, A" uniqKey="Hassan A" first="A." last="Hassan">A. Hassan</name><affiliation><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University</nlm:aff></affiliation></author><author><name sortKey="Azhar, E" sort="Azhar, E" uniqKey="Azhar E" first="E." last="Azhar">E. Azhar</name><affiliation><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University</nlm:aff></affiliation><affiliation><nlm:aff id="aff0010">Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University</nlm:aff></affiliation></author><author><name sortKey="Hashem, A" sort="Hashem, A" uniqKey="Hashem A" first="A." last="Hashem">A. Hashem</name><affiliation><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University</nlm:aff></affiliation><affiliation><nlm:aff id="aff0015">Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">7128511</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128511</idno><idno type="RBID">PMC:7128511</idno><idno type="doi">10.1016/j.jiph.2018.10.115</idno><idno type="pmid">NONE</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000609</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000609</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development and validation of different indirect ELISAs for MERS-CoV serological testing</title><author><name sortKey="Al Amri, S" sort="Al Amri, S" uniqKey="Al Amri S" first="S." last="Al-Amri">S. Al-Amri</name><affiliation><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University</nlm:aff></affiliation></author><author><name sortKey="Al Subhi, T" sort="Al Subhi, T" uniqKey="Al Subhi T" first="T." last="Al-Subhi">T. Al-Subhi</name><affiliation><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University</nlm:aff></affiliation></author><author><name sortKey="Hassan, A" sort="Hassan, A" uniqKey="Hassan A" first="A." last="Hassan">A. Hassan</name><affiliation><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University</nlm:aff></affiliation></author><author><name sortKey="Azhar, E" sort="Azhar, E" uniqKey="Azhar E" first="E." last="Azhar">E. Azhar</name><affiliation><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University</nlm:aff></affiliation><affiliation><nlm:aff id="aff0010">Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University</nlm:aff></affiliation></author><author><name sortKey="Hashem, A" sort="Hashem, A" uniqKey="Hashem A" first="A." last="Hashem">A. Hashem</name><affiliation><nlm:aff id="aff0005">Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University</nlm:aff></affiliation><affiliation><nlm:aff id="aff0015">Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University</nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Infection and Public Health</title><idno type="ISSN">1876-0341</idno><idno type="eISSN">1876-035X</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader></TEI><pmc article-type="abstract"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Infect Public Health</journal-id><journal-id journal-id-type="iso-abbrev">J Infect Public Health</journal-id><journal-title-group><journal-title>Journal of Infection and Public Health</journal-title></journal-title-group><issn pub-type="ppub">1876-0341</issn><issn pub-type="epub">1876-035X</issn><publisher><publisher-name>Published by Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmc">7128511</article-id><article-id pub-id-type="publisher-id">S1876-0341(18)30285-5</article-id><article-id pub-id-type="doi">10.1016/j.jiph.2018.10.115</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Development and validation of different indirect ELISAs for MERS-CoV serological testing</article-title></title-group><contrib-group><contrib contrib-type="author" id="aut0005"><name><surname>Al-Amri</surname><given-names>S.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author" id="aut0010"><name><surname>Al-subhi</surname><given-names>T.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="aut0015"><name><surname>Hassan</surname><given-names>A.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="aut0020"><name><surname>Azhar</surname><given-names>E.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref><xref rid="aff0010" ref-type="aff">2</xref></contrib><contrib contrib-type="author" id="aut0025"><name><surname>Hashem</surname><given-names>A.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref><xref rid="aff0015" ref-type="aff">3</xref></contrib></contrib-group><aff id="aff0005"><label>1</label>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University</aff><aff id="aff0010"><label>2</label>Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University</aff><aff id="aff0015"><label>3</label>Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author.</corresp></author-notes><pub-date pub-type="pmc-release"><day>8</day><month>1</month><year>2019</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub" iso-8601-date="2019-02-01"><season>January-February</season><year>2019</year></pub-date><pub-date pub-type="epub"><day>8</day><month>1</month><year>2019</year></pub-date><volume>12</volume><issue>1</issue><fpage>142</fpage><lpage>142</lpage><permissions><copyright-statement>Copyright © 2018 Published by Elsevier Ltd.</copyright-statement><copyright-year>2018</copyright-year><copyright-holder></copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions></article-meta></front><body><p id="par0005"><bold>Introduction:</bold> Since 2012, MERS-CoV has caused up to 2,220 cases and 790 deaths in 27 countries with Saudi Arabia being the most affected country with ∼83.1% of the cases and ∼38.8% local death rate. Current serological assays such as microneutralization (MN), plaque reduction neutralization, immunofluorescence, protein microarray or pseudoparticle neutralization assays rely on handling of live MERS-CoV in high containment laboratories or need for expensive and special equipment and reagents and highly trained personnel which represent a technical hurdle for most laboratories in resource-limited MERS-CoV endemic countries. Here, we developed, compared and evaluated three different indirect ELISAs based on different MERS-CoV proteins.</p><p id="par0010"><bold>Materials and Methods:</bold> MERS-CoV nucleocapsid protein (rN), spike (rS) ectodomain (amino acids 1-1297) and rS1 subunit (amino acids 1-725) proteins were expressed, purified and used for ELISA assay development. Assays were developed using checkerboard titration and finally validated with a total of 353 serum samples collected between 2014 and 2017 from high-risk groups (79 seropositive and 274 seronegative samples) in comparison with MN assay.</p><p id="par0015"><bold>Results:</bold> Three different ELISAs were developed using MERS-CoV rN, rS1 and rS, and cut-off values were found to be 0.30 for rN-ELISA, 0.26 for rS1-ELISA, and 0.34 for rS-ELISA. Upon validation, both rS1 and rS ELISAs maintained high sensitivity and specificity (≥90%) across a wider range of OD values compared to rN-ELISA. Moreover, rS1- and rS-based ELISAs showed better agreement and correlation with MN assay in contrast to rN-ELISA.</p><p id="par0020"><bold>Conclusions:</bold> Our data suggest that rS1-ELISA and/or rS-ELISA are more reliable than rN-ELISA as they showed higher sensitivity, specificity, agreement and correlation with MN assay. They could be used independently or in combination in large-scale and high-throughput seroepidemiological screening in MERS-CoV endemic regions as they do not require high containment laboratories and could be adapted easily by any lab.</p></body></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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