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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of Different Animal Models for Middle East Respiratory Syndrome-Coronavirus (MERS-CoV) Infection</title><author><name sortKey="Vergara Alert, J" sort="Vergara Alert, J" uniqKey="Vergara Alert J" first="J." last="Vergara-Alert">J. Vergara-Alert</name><affiliation><nlm:aff id="aff1">Centre de Recerca en Sanitat Animal (CReSA–IRTA), Spain</nlm:aff></affiliation></author><author><name sortKey="Van Den Brand, J" sort="Van Den Brand, J" uniqKey="Van Den Brand J" first="J." last="Van Den Brand">J. Van Den Brand</name><affiliation><nlm:aff id="aff2">Department of Viroscience, Erasmus Medical Centre, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Widagdo, W" sort="Widagdo, W" uniqKey="Widagdo W" first="W." last="Widagdo">W. Widagdo</name><affiliation><nlm:aff id="aff2">Department of Viroscience, Erasmus Medical Centre, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Mu Oz, M" sort="Mu Oz, M" uniqKey="Mu Oz M" first="M." last="Mu Oz">M. Mu Oz</name><affiliation><nlm:aff id="aff1">Centre de Recerca en Sanitat Animal (CReSA–IRTA), Spain</nlm:aff></affiliation></author><author><name sortKey="Solanes, D" sort="Solanes, D" uniqKey="Solanes D" first="D." last="Solanes">D. Solanes</name><affiliation><nlm:aff id="aff1">Centre de Recerca en Sanitat Animal (CReSA–IRTA), Spain</nlm:aff></affiliation></author><author><name sortKey="Bensaid, A" sort="Bensaid, A" uniqKey="Bensaid A" first="A." last="Bensaid">A. Bensaid</name><affiliation><nlm:aff id="aff1">Centre de Recerca en Sanitat Animal (CReSA–IRTA), Spain</nlm:aff></affiliation></author><author><name sortKey="Haagmans, B" sort="Haagmans, B" uniqKey="Haagmans B" first="B." last="Haagmans">B. Haagmans</name><affiliation><nlm:aff id="aff2">Department of Viroscience, Erasmus Medical Centre, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Segales, J" sort="Segales, J" uniqKey="Segales J" first="J." last="Segalés">J. Segalés</name><affiliation><nlm:aff id="aff1">Centre de Recerca en Sanitat Animal (CReSA–IRTA), Spain</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Spain</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">7130430</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130430</idno><idno type="RBID">PMC:7130430</idno><idno type="doi">10.1016/j.jcpa.2016.11.035</idno><idno type="pmid">NONE</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000604</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000604</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development of Different Animal Models for Middle East Respiratory Syndrome-Coronavirus (MERS-CoV) Infection</title><author><name sortKey="Vergara Alert, J" sort="Vergara Alert, J" uniqKey="Vergara Alert J" first="J." last="Vergara-Alert">J. Vergara-Alert</name><affiliation><nlm:aff id="aff1">Centre de Recerca en Sanitat Animal (CReSA–IRTA), Spain</nlm:aff></affiliation></author><author><name sortKey="Van Den Brand, J" sort="Van Den Brand, J" uniqKey="Van Den Brand J" first="J." last="Van Den Brand">J. Van Den Brand</name><affiliation><nlm:aff id="aff2">Department of Viroscience, Erasmus Medical Centre, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Widagdo, W" sort="Widagdo, W" uniqKey="Widagdo W" first="W." last="Widagdo">W. Widagdo</name><affiliation><nlm:aff id="aff2">Department of Viroscience, Erasmus Medical Centre, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Mu Oz, M" sort="Mu Oz, M" uniqKey="Mu Oz M" first="M." last="Mu Oz">M. Mu Oz</name><affiliation><nlm:aff id="aff1">Centre de Recerca en Sanitat Animal (CReSA–IRTA), Spain</nlm:aff></affiliation></author><author><name sortKey="Solanes, D" sort="Solanes, D" uniqKey="Solanes D" first="D." last="Solanes">D. Solanes</name><affiliation><nlm:aff id="aff1">Centre de Recerca en Sanitat Animal (CReSA–IRTA), Spain</nlm:aff></affiliation></author><author><name sortKey="Bensaid, A" sort="Bensaid, A" uniqKey="Bensaid A" first="A." last="Bensaid">A. Bensaid</name><affiliation><nlm:aff id="aff1">Centre de Recerca en Sanitat Animal (CReSA–IRTA), Spain</nlm:aff></affiliation></author><author><name sortKey="Haagmans, B" sort="Haagmans, B" uniqKey="Haagmans B" first="B." last="Haagmans">B. Haagmans</name><affiliation><nlm:aff id="aff2">Department of Viroscience, Erasmus Medical Centre, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Segales, J" sort="Segales, J" uniqKey="Segales J" first="J." last="Segalés">J. Segalés</name><affiliation><nlm:aff id="aff1">Centre de Recerca en Sanitat Animal (CReSA–IRTA), Spain</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Spain</nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Comparative Pathology</title><idno type="ISSN">0021-9975</idno><idno type="eISSN">1532-3129</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader></TEI><pmc article-type="abstract"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Comp Pathol</journal-id><journal-id journal-id-type="iso-abbrev">J. Comp. Pathol</journal-id><journal-title-group><journal-title>Journal of Comparative Pathology</journal-title></journal-title-group><issn pub-type="ppub">0021-9975</issn><issn pub-type="epub">1532-3129</issn><publisher><publisher-name>Published by Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmc">7130430</article-id><article-id pub-id-type="publisher-id">S0021-9975(16)30224-9</article-id><article-id pub-id-type="doi">10.1016/j.jcpa.2016.11.035</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Development of Different Animal Models for Middle East Respiratory Syndrome-Coronavirus (MERS-CoV) Infection</article-title></title-group><contrib-group><contrib contrib-type="author" id="au1"><name><surname>Vergara-Alert</surname><given-names>J.</given-names></name><xref rid="aff1" ref-type="aff">∗</xref></contrib><contrib contrib-type="author" id="au2"><name><surname>Van den Brand</surname><given-names>J.</given-names></name><xref rid="aff2" ref-type="aff">†</xref></contrib><contrib contrib-type="author" id="au3"><name><surname>Widagdo</surname><given-names>W.</given-names></name><xref rid="aff2" ref-type="aff">†</xref></contrib><contrib contrib-type="author" id="au4"><name><surname>Muñoz</surname><given-names>M.</given-names></name><xref rid="aff1" ref-type="aff">∗</xref></contrib><contrib contrib-type="author" id="au5"><name><surname>Solanes</surname><given-names>D.</given-names></name><xref rid="aff1" ref-type="aff">∗</xref></contrib><contrib contrib-type="author" id="au6"><name><surname>Bensaid</surname><given-names>A.</given-names></name><xref rid="aff1" ref-type="aff">∗</xref></contrib><contrib contrib-type="author" id="au7"><name><surname>Haagmans</surname><given-names>B.</given-names></name><xref rid="aff2" ref-type="aff">†</xref></contrib><contrib contrib-type="author" id="au8"><name><surname>Segalés</surname><given-names>J.</given-names></name><xref rid="aff1" ref-type="aff">∗</xref><xref rid="aff3" ref-type="aff">‡</xref></contrib></contrib-group><aff id="aff1"><label>∗</label>Centre de Recerca en Sanitat Animal (CReSA–IRTA), Spain</aff><aff id="aff2"><label>†</label>Department of Viroscience, Erasmus Medical Centre, The Netherlands</aff><aff id="aff3"><label>‡</label>Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Spain</aff><pub-date pub-type="pmc-release"><day>31</day><month>1</month><year>2017</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>1</month><year>2017</year></pub-date><pub-date pub-type="epub"><day>31</day><month>1</month><year>2017</year></pub-date><volume>156</volume><issue>1</issue><fpage>66</fpage><lpage>66</lpage><permissions><copyright-statement>Copyright © 2016 Published by Elsevier Ltd.</copyright-statement><copyright-year>2016</copyright-year><copyright-holder></copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions></article-meta></front><body><p id="p0010"><bold>Introduction:</bold> Middle East respiratory syndrome-coronavirus (MERS-CoV) emerged in 2012 as a causative agent of severe pneumonia and mortality in man. Further research indicated that an animal reservoir for MERS-CoV did exist (dromedary camels). Since other animal species susceptible to this viral infection cannot be ruled out, the aim of this work was to test four different species for their susceptibility to MERS-CoV and potential as an animal model.</p><p id="p0015"><bold>Materials and Methods:</bold> A total of 14 pigs, 14 sheep, eight horses (ponies) and eight llamas were inoculated intranasally with 10<sup>7</sup> TCID<sub>50</sub>/animal. Four pigs and four sheep were killed on day 2 post inoculation (pi) and four animals of each species were killed on days 4 and 24 pi. A complete necropsy examination was performed on all animals and histopathology and immunohistochemistry was performed on a set of tissues, with emphasis on the respiratory tract. Virus isolation and RT-PCR to detect MERS-CoV was performed on nasal swabs collected at 1, 2, 3, 5, 7, 10, 15 and 26 days pi.</p><p id="p0020"><bold>Results:</bold> Only llamas showed clinical signs consisting of moderate nostril mucus secretion. Inflammation and necrosis was predominantly seen in the nasal respiratory epithelium of llamas and pigs. Only pigs and llamas had positive virus isolation and RT-PCR results in nasal swabs variably from days 1 to 10.</p><p id="p0025"><bold>Conclusions:</bold> Pigs and llamas are susceptible to infection with MERS-CoV and might be used as potential models of subclinical infection with this virus.</p></body></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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