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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Respiratory Infection Routes of MERS-CoV in Rabbits</title><author><name sortKey="Van Den Brand, J M A" sort="Van Den Brand, J M A" uniqKey="Van Den Brand J" first="J. M. A." last="Van Den Brand">J. M. A. Van Den Brand</name><affiliation><nlm:aff id="aff1">Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Widagdo, W" sort="Widagdo, W" uniqKey="Widagdo W" first="W." last="Widagdo">W. Widagdo</name><affiliation><nlm:aff id="aff1">Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="De Waal, L" sort="De Waal, L" uniqKey="De Waal L" first="L." last="De Waal">L. De Waal</name><affiliation><nlm:aff id="aff2">Viroclinics, Biosciences BV, Rotterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Schipper, D" sort="Schipper, D" uniqKey="Schipper D" first="D." last="Schipper">D. Schipper</name><affiliation><nlm:aff id="aff1">Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Van Amerongen, G" sort="Van Amerongen, G" uniqKey="Van Amerongen G" first="G." last="Van Amerongen">G. Van Amerongen</name><affiliation><nlm:aff id="aff2">Viroclinics, Biosciences BV, Rotterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Stittelaar, K" sort="Stittelaar, K" uniqKey="Stittelaar K" first="K." last="Stittelaar">K. Stittelaar</name><affiliation><nlm:aff id="aff2">Viroclinics, Biosciences BV, Rotterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Haagmans, B" sort="Haagmans, B" uniqKey="Haagmans B" first="B." last="Haagmans">B. Haagmans</name><affiliation><nlm:aff id="aff1">Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">7130173</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130173</idno><idno type="RBID">PMC:7130173</idno><idno type="doi">10.1016/j.jcpa.2016.11.076</idno><idno type="pmid">NONE</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000600</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000600</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Respiratory Infection Routes of MERS-CoV in Rabbits</title><author><name sortKey="Van Den Brand, J M A" sort="Van Den Brand, J M A" uniqKey="Van Den Brand J" first="J. M. A." last="Van Den Brand">J. M. A. Van Den Brand</name><affiliation><nlm:aff id="aff1">Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Widagdo, W" sort="Widagdo, W" uniqKey="Widagdo W" first="W." last="Widagdo">W. Widagdo</name><affiliation><nlm:aff id="aff1">Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="De Waal, L" sort="De Waal, L" uniqKey="De Waal L" first="L." last="De Waal">L. De Waal</name><affiliation><nlm:aff id="aff2">Viroclinics, Biosciences BV, Rotterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Schipper, D" sort="Schipper, D" uniqKey="Schipper D" first="D." last="Schipper">D. Schipper</name><affiliation><nlm:aff id="aff1">Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Van Amerongen, G" sort="Van Amerongen, G" uniqKey="Van Amerongen G" first="G." last="Van Amerongen">G. Van Amerongen</name><affiliation><nlm:aff id="aff2">Viroclinics, Biosciences BV, Rotterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Stittelaar, K" sort="Stittelaar, K" uniqKey="Stittelaar K" first="K." last="Stittelaar">K. Stittelaar</name><affiliation><nlm:aff id="aff2">Viroclinics, Biosciences BV, Rotterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Haagmans, B" sort="Haagmans, B" uniqKey="Haagmans B" first="B." last="Haagmans">B. Haagmans</name><affiliation><nlm:aff id="aff1">Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands</nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Comparative Pathology</title><idno type="ISSN">0021-9975</idno><idno type="eISSN">1532-3129</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader></TEI><pmc article-type="abstract"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Comp Pathol</journal-id><journal-id journal-id-type="iso-abbrev">J. Comp. Pathol</journal-id><journal-title-group><journal-title>Journal of Comparative Pathology</journal-title></journal-title-group><issn pub-type="ppub">0021-9975</issn><issn pub-type="epub">1532-3129</issn><publisher><publisher-name>Published by Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmc">7130173</article-id><article-id pub-id-type="publisher-id">S0021-9975(16)30265-1</article-id><article-id pub-id-type="doi">10.1016/j.jcpa.2016.11.076</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Respiratory Infection Routes of MERS-CoV in Rabbits</article-title></title-group><contrib-group><contrib contrib-type="author" id="au1"><name><surname>Van den Brand</surname><given-names>J.M.A.</given-names></name><xref rid="aff1" ref-type="aff">∗</xref></contrib><contrib contrib-type="author" id="au2"><name><surname>Widagdo</surname><given-names>W.</given-names></name><xref rid="aff1" ref-type="aff">∗</xref></contrib><contrib contrib-type="author" id="au3"><name><surname>de Waal</surname><given-names>L.</given-names></name><xref rid="aff2" ref-type="aff">†</xref></contrib><contrib contrib-type="author" id="au4"><name><surname>Schipper</surname><given-names>D.</given-names></name><xref rid="aff1" ref-type="aff">∗</xref></contrib><contrib contrib-type="author" id="au5"><name><surname>van Amerongen</surname><given-names>G.</given-names></name><xref rid="aff2" ref-type="aff">†</xref></contrib><contrib contrib-type="author" id="au6"><name><surname>Stittelaar</surname><given-names>K.</given-names></name><xref rid="aff2" ref-type="aff">†</xref></contrib><contrib contrib-type="author" id="au7"><name><surname>Haagmans</surname><given-names>B.</given-names></name><xref rid="aff1" ref-type="aff">∗</xref></contrib></contrib-group><aff id="aff1"><label>∗</label>Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands</aff><aff id="aff2"><label>†</label>Viroclinics, Biosciences BV, Rotterdam, The Netherlands</aff><pub-date pub-type="pmc-release"><day>31</day><month>1</month><year>2017</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>1</month><year>2017</year></pub-date><pub-date pub-type="epub"><day>31</day><month>1</month><year>2017</year></pub-date><volume>156</volume><issue>1</issue><fpage>79</fpage><lpage>79</lpage><permissions><copyright-statement>Copyright © 2016 Published by Elsevier Ltd.</copyright-statement><copyright-year>2016</copyright-year><copyright-holder></copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions></article-meta></front><body><p id="p0010"><bold>Introduction</bold>: Middle East respiratory coronavirus (MERS-CoV) causes respiratory disease in man, often resulting in severe or even fatal pneumonia. Rabbits are susceptible to MERS-CoV after simultaneous intratracheal and intranasal inoculation. Therefore, rabbits are a useful animal model for study of the pathogenesis of infection and preventive and therapeutic countermeasures for human patients. However, virus tropism and the course of disease in rabbits infected only via the nose are unknown.</p><p id="p0015"><bold>Materials and Methods</bold>: To compare different inoculation routes, 12 rabbits were divided into four groups and inoculated with MERS-CoV as follows: intratracheal with 3 ml and intranasal with 0.2 ml, intranasal with 1 ml or with 0.2 ml of MERS-CoV, or with 1 ml of PBS. Swabs were taken daily and at 4 days after inoculation the animals were killed, subjected to necropsy examination and sampled for histopathology, immunohistochemistry and virology.</p><p id="p0020"><bold>Results</bold>: Rabbits inoculated via the intranasal route with 1 ml of virus had infectious virus titres and high viral loads by PCR in nose swabs from 1 to 4 days after inoculation and in nasal turbinates and lungs at necropsy examination. Immunohistochemistry demonstrated virus antigen in nasal and pulmonary epithelial cells associated with inflammation.</p><p id="p0025"><bold>Conclusions</bold>: Rabbits can be infected via the intranasal route and have comparable virus tropism, virus load and inflammation as is seen in rabbits with additional intratracheal inoculation. Therefore, rabbits inoculated via the intranasal route can be used as a model to study the pathogenesis and intervention strategies for human disease.</p></body></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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