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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) shows poor replication and weak induction of antiviral responses in human monocyte-derived macrophages and dendritic cells</title><author><name sortKey="Tynell, J" sort="Tynell, J" uniqKey="Tynell J" first="J." last="Tynell">J. Tynell</name><affiliation><nlm:aff id="aff0005">National Institute for Health and Welfare (THL), Helsinki, Finland</nlm:aff></affiliation></author><author><name sortKey="Westenius, V" sort="Westenius, V" uniqKey="Westenius V" first="V." last="Westenius">V. Westenius</name><affiliation><nlm:aff id="aff0005">National Institute for Health and Welfare (THL), Helsinki, Finland</nlm:aff></affiliation></author><author><name sortKey="Ronkko, E" sort="Ronkko, E" uniqKey="Ronkko E" first="E." last="Rönkkö">E. Rönkkö</name><affiliation><nlm:aff id="aff0005">National Institute for Health and Welfare (THL), Helsinki, Finland</nlm:aff></affiliation></author><author><name sortKey="Munster, V J" sort="Munster, V J" uniqKey="Munster V" first="V. J." last="Munster">V. J. Munster</name><affiliation><nlm:aff id="aff0010">National Institute of Allergy and Infectious Diseases, Hamilton, MT, USA</nlm:aff></affiliation></author><author><name sortKey="Melen, K" sort="Melen, K" uniqKey="Melen K" first="K." last="Melén">K. Melén</name><affiliation><nlm:aff id="aff0005">National Institute for Health and Welfare (THL), Helsinki, Finland</nlm:aff></affiliation></author><author><name sortKey="Osterlund, P" sort="Osterlund, P" uniqKey="Osterlund P" first="P." last="Österlund">P. Österlund</name><affiliation><nlm:aff id="aff0005">National Institute for Health and Welfare (THL), Helsinki, Finland</nlm:aff></affiliation></author><author><name sortKey="Julkunen, I" sort="Julkunen, I" uniqKey="Julkunen I" first="I." last="Julkunen">I. Julkunen</name><affiliation><nlm:aff id="aff0005">National Institute for Health and Welfare (THL), Helsinki, Finland</nlm:aff></affiliation><affiliation><nlm:aff id="aff0015">University of Turku, Turku, Finland</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">7128158</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128158</idno><idno type="RBID">PMC:7128158</idno><idno type="doi">10.1016/j.jcv.2015.07.134</idno><idno type="pmid">NONE</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000595</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000595</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Middle East respiratory syndrome coronavirus (MERS-CoV) shows poor replication and weak induction of antiviral responses in human monocyte-derived macrophages and dendritic cells</title><author><name sortKey="Tynell, J" sort="Tynell, J" uniqKey="Tynell J" first="J." last="Tynell">J. Tynell</name><affiliation><nlm:aff id="aff0005">National Institute for Health and Welfare (THL), Helsinki, Finland</nlm:aff></affiliation></author><author><name sortKey="Westenius, V" sort="Westenius, V" uniqKey="Westenius V" first="V." last="Westenius">V. Westenius</name><affiliation><nlm:aff id="aff0005">National Institute for Health and Welfare (THL), Helsinki, Finland</nlm:aff></affiliation></author><author><name sortKey="Ronkko, E" sort="Ronkko, E" uniqKey="Ronkko E" first="E." last="Rönkkö">E. Rönkkö</name><affiliation><nlm:aff id="aff0005">National Institute for Health and Welfare (THL), Helsinki, Finland</nlm:aff></affiliation></author><author><name sortKey="Munster, V J" sort="Munster, V J" uniqKey="Munster V" first="V. J." last="Munster">V. J. Munster</name><affiliation><nlm:aff id="aff0010">National Institute of Allergy and Infectious Diseases, Hamilton, MT, USA</nlm:aff></affiliation></author><author><name sortKey="Melen, K" sort="Melen, K" uniqKey="Melen K" first="K." last="Melén">K. Melén</name><affiliation><nlm:aff id="aff0005">National Institute for Health and Welfare (THL), Helsinki, Finland</nlm:aff></affiliation></author><author><name sortKey="Osterlund, P" sort="Osterlund, P" uniqKey="Osterlund P" first="P." last="Österlund">P. Österlund</name><affiliation><nlm:aff id="aff0005">National Institute for Health and Welfare (THL), Helsinki, Finland</nlm:aff></affiliation></author><author><name sortKey="Julkunen, I" sort="Julkunen, I" uniqKey="Julkunen I" first="I." last="Julkunen">I. Julkunen</name><affiliation><nlm:aff id="aff0005">National Institute for Health and Welfare (THL), Helsinki, Finland</nlm:aff></affiliation><affiliation><nlm:aff id="aff0015">University of Turku, Turku, Finland</nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Clinical Virology</title><idno type="ISSN">1386-6532</idno><idno type="eISSN">1873-5967</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader></TEI><pmc article-type="abstract"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Virol</journal-id><journal-id journal-id-type="iso-abbrev">J. Clin. Virol</journal-id><journal-title-group><journal-title>Journal of Clinical Virology</journal-title></journal-title-group><issn pub-type="ppub">1386-6532</issn><issn pub-type="epub">1873-5967</issn><publisher><publisher-name>Published by Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmc">7128158</article-id><article-id pub-id-type="publisher-id">S1386-6532(15)00434-5</article-id><article-id pub-id-type="doi">10.1016/j.jcv.2015.07.134</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Middle East respiratory syndrome coronavirus (MERS-CoV) shows poor replication and weak induction of antiviral responses in human monocyte-derived macrophages and dendritic cells</article-title></title-group><contrib-group><contrib contrib-type="author" id="aut0005"><name><surname>Tynell</surname><given-names>J.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author" id="aut0010"><name><surname>Westenius</surname><given-names>V.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="aut0015"><name><surname>Rönkkö</surname><given-names>E.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="aut0020"><name><surname>Munster</surname><given-names>V.J.</given-names></name><xref rid="aff0010" ref-type="aff">2</xref></contrib><contrib contrib-type="author" id="aut0025"><name><surname>Melén</surname><given-names>K.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="aut0030"><name><surname>Österlund</surname><given-names>P.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="aut0035"><name><surname>Julkunen</surname><given-names>I.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref><xref rid="aff0015" ref-type="aff">3</xref></contrib></contrib-group><aff id="aff0005"><label>1</label>National Institute for Health and Welfare (THL), Helsinki, Finland</aff><aff id="aff0010"><label>2</label>National Institute of Allergy and Infectious Diseases, Hamilton, MT, USA</aff><aff id="aff0015"><label>3</label>University of Turku, Turku, Finland</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author.</corresp></author-notes><pub-date pub-type="pmc-release"><day>18</day><month>8</month><year>2015</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>9</month><year>2015</year></pub-date><pub-date pub-type="epub"><day>18</day><month>8</month><year>2015</year></pub-date><volume>70</volume><fpage>S56</fpage><lpage>S57</lpage><permissions><copyright-statement>Copyright © 2015 Published by Elsevier B.V.</copyright-statement><copyright-year>2015</copyright-year><copyright-holder></copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions></article-meta></front><body><p id="par0005"><bold>Background:</bold> The Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel coronavirus causing severe acute respiratory infections associated with high mortality. In the present study we assessed the ability of MERS-CoV to replicate and induce innate immunity in human monocyte-derived macrophages and dendritic cells (DCs) and compared these responses to those of severe acute respiratory syndrome coronavirus (SARS-CoV).</p><p id="par0010"><bold>Methods:</bold> Human monocyte-derived macrophages and DCs as well as A549, Calu-3 and Vero E6 cells were infected with MERS-CoV and SARS-CoV at MOI 1. Viral replication and cytokine production were measured by endpoint dilution assay, qRT-PCR and Western blot.</p><p id="par0015"><bold>Results:</bold> Assessments of viral RNA levels by quantitative reverse transcription polymerase chain reaction (qRT-PCR) from infected cells during a 48 h infection indicated that like SARS-CoV, MERS-CoV can infect macrophages and DCs, but both viruses showed an impaired ability to replicate in these cells. This observation was supported by Western blot analysis of cell lysates showing no increase in viral N protein amounts during infection. qRT-PCR analysis of cytokine and MxA mRNA levels showed that MERS-CoV infection results in some induction of interferon lambda 1, CXCL10 and MxA mRNAs in both macrophages and DCs, whereas almost no such induction was observed by SARS-CoV. However, both viruses replicated very well in human lung epithelial Calu-3 cells followed by strong induction of interferon lambda 1, CXCL10 and MxA mRNAs.</p><p id="par0020"><bold>Conclusions:</bold> Our data suggest that SARS-CoV and MERS-CoV replicate extremely poorly in human macrophages and DCs and thus the viruses are incapable of inducing a strong host innate immune response. However, we observed a clear difference between MERS-CoV and SARS-CoV in their ability to activate host innate immune responses in human macrophages and DCs, which may contribute to the pathogenesis of infection. This work was funded by the Sigrid Juselius Foundation and the Academy of Finland.</p></body></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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