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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A poxvirus-based vaccine reduces virus excretion after MERS coronavirus infection in dromedary camels</title><author><name sortKey="Stalin Raj, V" sort="Stalin Raj, V" uniqKey="Stalin Raj V" first="V." last="Stalin Raj">V. Stalin Raj</name><affiliation><nlm:aff id="aff0005">Erasmus Medical center, Rotterdam, Netherlands</nlm:aff></affiliation></author><author><name sortKey="Van Den Brand, J M A" sort="Van Den Brand, J M A" uniqKey="Van Den Brand J" first="J. M. A." last="Van Den Brand">J. M. A. Van Den Brand</name><affiliation><nlm:aff id="aff0005">Erasmus Medical center, Rotterdam, Netherlands</nlm:aff></affiliation></author><author><name sortKey="Volz, A" sort="Volz, A" uniqKey="Volz A" first="A." last="Volz">A. Volz</name><affiliation><nlm:aff id="aff0010">LMU University of Munich, Munich, Germany</nlm:aff></affiliation></author><author><name sortKey="Wohlsein, P" sort="Wohlsein, P" uniqKey="Wohlsein P" first="P." last="Wohlsein">P. Wohlsein</name><affiliation><nlm:aff id="aff0015">University of Veterinary Medicine, Hanover, Germany</nlm:aff></affiliation></author><author><name sortKey="Smits, S L" sort="Smits, S L" uniqKey="Smits S" first="S. L." last="Smits">S. L. Smits</name><affiliation><nlm:aff id="aff0005">Erasmus Medical center, Rotterdam, Netherlands</nlm:aff></affiliation></author><author><name sortKey="Okba, N" sort="Okba, N" uniqKey="Okba N" first="N." last="Okba">N. Okba</name><affiliation><nlm:aff id="aff0005">Erasmus Medical center, Rotterdam, Netherlands</nlm:aff></affiliation></author><author><name sortKey="Fux, R" sort="Fux, R" uniqKey="Fux R" first="R." last="Fux">R. Fux</name><affiliation><nlm:aff id="aff0010">LMU University of Munich, Munich, Germany</nlm:aff></affiliation></author><author><name sortKey="Moise Bensaid, A" sort="Moise Bensaid, A" uniqKey="Moise Bensaid A" first="A." last="Moise Bensaid">A. Moise Bensaid</name><affiliation><nlm:aff id="aff0020">Centre de Recerca en Sanitat Animal, Barcelona, Spain</nlm:aff></affiliation></author><author><name sortKey="Solanes Foz, D" sort="Solanes Foz, D" uniqKey="Solanes Foz D" first="D." last="Solanes Foz">D. Solanes Foz</name><affiliation><nlm:aff id="aff0020">Centre de Recerca en Sanitat Animal, Barcelona, Spain</nlm:aff></affiliation></author><author><name sortKey="Kuiken, T" sort="Kuiken, T" uniqKey="Kuiken T" first="T." last="Kuiken">T. Kuiken</name><affiliation><nlm:aff id="aff0005">Erasmus Medical center, Rotterdam, Netherlands</nlm:aff></affiliation></author><author><name sortKey="Baumg Rtner, W" sort="Baumg Rtner, W" uniqKey="Baumg Rtner W" first="W." last="Baumg Rtner">W. Baumg Rtner</name><affiliation><nlm:aff id="aff0015">University of Veterinary Medicine, Hanover, Germany</nlm:aff></affiliation></author><author><name sortKey="Segales, J" sort="Segales, J" uniqKey="Segales J" first="J." last="Segalés">J. Segalés</name><affiliation><nlm:aff id="aff0020">Centre de Recerca en Sanitat Animal, Barcelona, Spain</nlm:aff></affiliation></author><author><name sortKey="Sutter, G" sort="Sutter, G" uniqKey="Sutter G" first="G." last="Sutter">G. Sutter</name><affiliation><nlm:aff id="aff0010">LMU University of Munich, Munich, Germany</nlm:aff></affiliation></author><author><name sortKey="Osterhaus, A D M E" sort="Osterhaus, A D M E" uniqKey="Osterhaus A" first="A. D. M. E." last="Osterhaus">A. D. M. E. Osterhaus</name><affiliation><nlm:aff id="aff0015">University of Veterinary Medicine, Hanover, Germany</nlm:aff></affiliation></author><author><name sortKey="L Haagmans, B" sort="L Haagmans, B" uniqKey="L Haagmans B" first="B." last="L Haagmans">B. L Haagmans</name><affiliation><nlm:aff id="aff0005">Erasmus Medical center, Rotterdam, Netherlands</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">7128089</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128089</idno><idno type="RBID">PMC:7128089</idno><idno type="doi">10.1016/j.ijid.2016.02.898</idno><idno type="pmid">NONE</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000578</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000578</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A poxvirus-based vaccine reduces virus excretion after MERS coronavirus infection in dromedary camels</title><author><name sortKey="Stalin Raj, V" sort="Stalin Raj, V" uniqKey="Stalin Raj V" first="V." last="Stalin Raj">V. Stalin Raj</name><affiliation><nlm:aff id="aff0005">Erasmus Medical center, Rotterdam, Netherlands</nlm:aff></affiliation></author><author><name sortKey="Van Den Brand, J M A" sort="Van Den Brand, J M A" uniqKey="Van Den Brand J" first="J. M. A." last="Van Den Brand">J. M. A. Van Den Brand</name><affiliation><nlm:aff id="aff0005">Erasmus Medical center, Rotterdam, Netherlands</nlm:aff></affiliation></author><author><name sortKey="Volz, A" sort="Volz, A" uniqKey="Volz A" first="A." last="Volz">A. Volz</name><affiliation><nlm:aff id="aff0010">LMU University of Munich, Munich, Germany</nlm:aff></affiliation></author><author><name sortKey="Wohlsein, P" sort="Wohlsein, P" uniqKey="Wohlsein P" first="P." last="Wohlsein">P. Wohlsein</name><affiliation><nlm:aff id="aff0015">University of Veterinary Medicine, Hanover, Germany</nlm:aff></affiliation></author><author><name sortKey="Smits, S L" sort="Smits, S L" uniqKey="Smits S" first="S. L." last="Smits">S. L. Smits</name><affiliation><nlm:aff id="aff0005">Erasmus Medical center, Rotterdam, Netherlands</nlm:aff></affiliation></author><author><name sortKey="Okba, N" sort="Okba, N" uniqKey="Okba N" first="N." last="Okba">N. Okba</name><affiliation><nlm:aff id="aff0005">Erasmus Medical center, Rotterdam, Netherlands</nlm:aff></affiliation></author><author><name sortKey="Fux, R" sort="Fux, R" uniqKey="Fux R" first="R." last="Fux">R. Fux</name><affiliation><nlm:aff id="aff0010">LMU University of Munich, Munich, Germany</nlm:aff></affiliation></author><author><name sortKey="Moise Bensaid, A" sort="Moise Bensaid, A" uniqKey="Moise Bensaid A" first="A." last="Moise Bensaid">A. Moise Bensaid</name><affiliation><nlm:aff id="aff0020">Centre de Recerca en Sanitat Animal, Barcelona, Spain</nlm:aff></affiliation></author><author><name sortKey="Solanes Foz, D" sort="Solanes Foz, D" uniqKey="Solanes Foz D" first="D." last="Solanes Foz">D. Solanes Foz</name><affiliation><nlm:aff id="aff0020">Centre de Recerca en Sanitat Animal, Barcelona, Spain</nlm:aff></affiliation></author><author><name sortKey="Kuiken, T" sort="Kuiken, T" uniqKey="Kuiken T" first="T." last="Kuiken">T. Kuiken</name><affiliation><nlm:aff id="aff0005">Erasmus Medical center, Rotterdam, Netherlands</nlm:aff></affiliation></author><author><name sortKey="Baumg Rtner, W" sort="Baumg Rtner, W" uniqKey="Baumg Rtner W" first="W." last="Baumg Rtner">W. Baumg Rtner</name><affiliation><nlm:aff id="aff0015">University of Veterinary Medicine, Hanover, Germany</nlm:aff></affiliation></author><author><name sortKey="Segales, J" sort="Segales, J" uniqKey="Segales J" first="J." last="Segalés">J. Segalés</name><affiliation><nlm:aff id="aff0020">Centre de Recerca en Sanitat Animal, Barcelona, Spain</nlm:aff></affiliation></author><author><name sortKey="Sutter, G" sort="Sutter, G" uniqKey="Sutter G" first="G." last="Sutter">G. Sutter</name><affiliation><nlm:aff id="aff0010">LMU University of Munich, Munich, Germany</nlm:aff></affiliation></author><author><name sortKey="Osterhaus, A D M E" sort="Osterhaus, A D M E" uniqKey="Osterhaus A" first="A. D. M. E." last="Osterhaus">A. D. M. E. Osterhaus</name><affiliation><nlm:aff id="aff0015">University of Veterinary Medicine, Hanover, Germany</nlm:aff></affiliation></author><author><name sortKey="L Haagmans, B" sort="L Haagmans, B" uniqKey="L Haagmans B" first="B." last="L Haagmans">B. L Haagmans</name><affiliation><nlm:aff id="aff0005">Erasmus Medical center, Rotterdam, Netherlands</nlm:aff></affiliation></author></analytic><series><title level="j">International Journal of Infectious Diseases</title><idno type="ISSN">1201-9712</idno><idno type="eISSN">1878-3511</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader></TEI><pmc article-type="abstract"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Int J Infect Dis</journal-id><journal-id journal-id-type="iso-abbrev">Int. J. Infect. Dis</journal-id><journal-title-group><journal-title>International Journal of Infectious Diseases</journal-title></journal-title-group><issn pub-type="ppub">1201-9712</issn><issn pub-type="epub">1878-3511</issn><publisher><publisher-name>Published by Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmc">7128089</article-id><article-id pub-id-type="publisher-id">S1201-9712(16)30877-3</article-id><article-id pub-id-type="doi">10.1016/j.ijid.2016.02.898</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>A poxvirus-based vaccine reduces virus excretion after MERS coronavirus infection in dromedary camels</article-title></title-group><contrib-group><contrib contrib-type="author" id="aut0005"><name><surname>Stalin Raj</surname><given-names>V.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author" id="aut0010"><name><surname>van den Brand</surname><given-names>J.M.A.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="aut0015"><name><surname>Volz</surname><given-names>A.</given-names></name><xref rid="aff0010" ref-type="aff">2</xref></contrib><contrib contrib-type="author" id="aut0020"><name><surname>Wohlsein</surname><given-names>P.</given-names></name><xref rid="aff0015" ref-type="aff">3</xref></contrib><contrib contrib-type="author" id="aut0025"><name><surname>Smits</surname><given-names>S.L.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="aut0030"><name><surname>Okba</surname><given-names>N.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="aut0035"><name><surname>Fux</surname><given-names>R.</given-names></name><xref rid="aff0010" ref-type="aff">2</xref></contrib><contrib contrib-type="author" id="aut0040"><name><surname>Moise Bensaid</surname><given-names>A.</given-names></name><xref rid="aff0020" ref-type="aff">4</xref></contrib><contrib contrib-type="author" id="aut0045"><name><surname>Solanes Foz</surname><given-names>D.</given-names></name><xref rid="aff0020" ref-type="aff">4</xref></contrib><contrib contrib-type="author" id="aut0050"><name><surname>Kuiken</surname><given-names>T.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="aut0055"><name><surname>Baumgärtner</surname><given-names>W.</given-names></name><xref rid="aff0015" ref-type="aff">3</xref></contrib><contrib contrib-type="author" id="aut0060"><name><surname>Segalés</surname><given-names>J.</given-names></name><xref rid="aff0020" ref-type="aff">4</xref></contrib><contrib contrib-type="author" id="aut0065"><name><surname>Sutter</surname><given-names>G.</given-names></name><xref rid="aff0010" ref-type="aff">2</xref></contrib><contrib contrib-type="author" id="aut0070"><name><surname>Osterhaus</surname><given-names>A.D.M.E.</given-names></name><xref rid="aff0015" ref-type="aff">3</xref></contrib><contrib contrib-type="author" id="aut0075"><name><surname>L Haagmans</surname><given-names>B.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref></contrib></contrib-group><aff id="aff0005"><label>1</label>Erasmus Medical center, Rotterdam, Netherlands</aff><aff id="aff0010"><label>2</label>LMU University of Munich, Munich, Germany</aff><aff id="aff0015"><label>3</label>University of Veterinary Medicine, Hanover, Germany</aff><aff id="aff0020"><label>4</label>Centre de Recerca en Sanitat Animal, Barcelona, Spain</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author.</corresp></author-notes><pub-date pub-type="pmc-release"><day>29</day><month>3</month><year>2016</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>4</month><year>2016</year></pub-date><pub-date pub-type="epub"><day>29</day><month>3</month><year>2016</year></pub-date><volume>45</volume><fpage>421</fpage><lpage>422</lpage><permissions><copyright-statement>Copyright © 2016 Published by Elsevier Ltd.</copyright-statement><copyright-year>2016</copyright-year><copyright-holder></copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions></article-meta></front><body><p id="par0005"><bold>Background</bold>: The recently emerged Middle East syndrome coronavirus (MERS-CoV) can cause severe and fatal respiratory diseases in humans. Antibodies against MESR-CoV can be found in camels in the Middle East but also outside this regionThe high prevalence of circulating MERS-CoV neutralizing antibodies in dromedary camels from different geographic regions may indicate wide circulation of MERS-CoV in camels. The ongoing MERS-CoV outbreak in the Middle East and the lack of treatment options or licensed vaccines is of great concern. Vaccination of camels could potentially prevent the spread of this virus.</p><p id="par0010"><bold>Methods & Materials</bold>: We vaccinated 4 dromedary camels twice with a 4 week interval with10<sup>8</sup> PFU MVA-S via both nostrils and intramuscularly. Four control animals received non-recombinant MVA (n=2) or PBS (n=2). Three weeks post-boost, all animals were tested for presence specific antibody responses by MERS-CoV ELSA and virus neutralization assay (VNT). Next, all camels were challenged with a high dose of MERS-CoV and to study the pathological changes, two animals per groups were euthanized and necropsies were performed at day 4 and 14 pi. The antibody response, and pathology were analyses by MERS-CoV-S or MVA ELISAs, -VNTs, qRT-PCR, virus titration, immunohistochemistry (IHC) and <italic>in situ</italic> hybridization (ISH).</p><p id="par0015"><bold>Results</bold>: All vaccinated animal developed detectable serum neutralizing MERS-CoV or MVA specific antibody titers 3 weeks post boost vaccination. No clinical signs were observed in MVA-S vaccinated animals but mild clinical sign and a runny nose were observed in control-vaccinated animals after virus challenge. Interestingly, significant reduction of infectious virus excreted and viral RNA transcripts in the vaccinated animals nose after MERS-CoV challenge was observed as compared with control animals, and these protection correlated with presence of neutralizing antibody to MERS-CoV. In addition, in the nose of MVA-S vaccinated animals at day 4 pi, a few MERS-CoV infected cells were detected by IHC and ISH as compared with control camels. Interestingly, sera from MVA-S vaccinated animals cross neutralized camelpox virus.</p><p id="par0020"><bold>Conclusion</bold>: Our results demonstrate that vaccination of camels with MVA-S confers protects against MERS-CoV infection. In addition, induction of MVA specific antibody cross neutralize camelpox virus, suggesting that MVA-MERS-S can be used as a dual vaccine in dromedary camels.</p></body></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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