Zika Virus: the Latest Newcomer
Identifieur interne : 000111 ( Pmc/Corpus ); précédent : 000110; suivant : 000112Zika Virus: the Latest Newcomer
Auteurs : Juan-Carlos Saiz ; Ángela Vázquez-Calvo ; Ana B. Blázquez ; Teresa Merino-Ramos ; Estela Escribano-Romero ; Miguel A. Martín-AcebesSource :
- Frontiers in Microbiology [ 1664-302X ] ; 2016.
Abstract
Since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: West Nile, Influenza A, avian flu, dengue, Chikungunya, SARS, MERS, Ebola, and now Zika, the latest newcomer. Zika virus (ZIKV), a flavivirus transmitted by
Url:
DOI: 10.3389/fmicb.2016.00496
PubMed: 27148186
PubMed Central: 4835484
Links to Exploration step
PMC:4835484Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Zika Virus: the Latest Newcomer</title><author><name sortKey="Saiz, Juan Carlos" sort="Saiz, Juan Carlos" uniqKey="Saiz J" first="Juan-Carlos" last="Saiz">Juan-Carlos Saiz</name></author><author><name sortKey="Vazquez Calvo, Angela" sort="Vazquez Calvo, Angela" uniqKey="Vazquez Calvo A" first="Ángela" last="Vázquez-Calvo">Ángela Vázquez-Calvo</name></author><author><name sortKey="Blazquez, Ana B" sort="Blazquez, Ana B" uniqKey="Blazquez A" first="Ana B." last="Blázquez">Ana B. Blázquez</name></author><author><name sortKey="Merino Ramos, Teresa" sort="Merino Ramos, Teresa" uniqKey="Merino Ramos T" first="Teresa" last="Merino-Ramos">Teresa Merino-Ramos</name></author><author><name sortKey="Escribano Romero, Estela" sort="Escribano Romero, Estela" uniqKey="Escribano Romero E" first="Estela" last="Escribano-Romero">Estela Escribano-Romero</name></author><author><name sortKey="Martin Acebes, Miguel A" sort="Martin Acebes, Miguel A" uniqKey="Martin Acebes M" first="Miguel A." last="Martín-Acebes">Miguel A. Martín-Acebes</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27148186</idno><idno type="pmc">4835484</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835484</idno><idno type="RBID">PMC:4835484</idno><idno type="doi">10.3389/fmicb.2016.00496</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000111</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000111</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Zika Virus: the Latest Newcomer</title><author><name sortKey="Saiz, Juan Carlos" sort="Saiz, Juan Carlos" uniqKey="Saiz J" first="Juan-Carlos" last="Saiz">Juan-Carlos Saiz</name></author><author><name sortKey="Vazquez Calvo, Angela" sort="Vazquez Calvo, Angela" uniqKey="Vazquez Calvo A" first="Ángela" last="Vázquez-Calvo">Ángela Vázquez-Calvo</name></author><author><name sortKey="Blazquez, Ana B" sort="Blazquez, Ana B" uniqKey="Blazquez A" first="Ana B." last="Blázquez">Ana B. Blázquez</name></author><author><name sortKey="Merino Ramos, Teresa" sort="Merino Ramos, Teresa" uniqKey="Merino Ramos T" first="Teresa" last="Merino-Ramos">Teresa Merino-Ramos</name></author><author><name sortKey="Escribano Romero, Estela" sort="Escribano Romero, Estela" uniqKey="Escribano Romero E" first="Estela" last="Escribano-Romero">Estela Escribano-Romero</name></author><author><name sortKey="Martin Acebes, Miguel A" sort="Martin Acebes, Miguel A" uniqKey="Martin Acebes M" first="Miguel A." last="Martín-Acebes">Miguel A. Martín-Acebes</name></author></analytic><series><title level="j">Frontiers in Microbiology</title><idno type="eISSN">1664-302X</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: West Nile, Influenza A, avian flu, dengue, Chikungunya, SARS, MERS, Ebola, and now Zika, the latest newcomer. Zika virus (ZIKV), a flavivirus transmitted by <italic>Aedes</italic> mosquitoes, was identified in 1947 in a sentinel monkey in Uganda, and later on in humans in Nigeria. The virus was mainly confined to the African continent until it was detected in south-east Asia the 1980’s, then in the Micronesia in 2007 and, more recently in the Americas in 2014, where it has displayed an explosive spread, as advised by the World Health Organization, which resulted in the infection of hundreds of thousands of people. ZIKV infection was characterized by causing a mild disease presented with fever, headache, rash, arthralgia, and conjunctivitis, with exceptional reports of an association with Guillain–Barre syndrome (GBS) and microcephaly. However, since the end of 2015, an increase in the number of GBS associated cases and an astonishing number of microcephaly in fetus and new-borns in Brazil have been related to ZIKV infection, raising serious worldwide public health concerns. Clarifying such worrisome relationships is, thus, a current unavoidable goal. Here, we extensively review what is currently known about ZIKV, from molecular biology, transmission routes, ecology, and epidemiology, to clinical manifestations, pathogenesis, diagnosis, prophylaxis, and public health.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Acosta, E G" uniqKey="Acosta E">E. G. Acosta</name></author><author><name sortKey="Kumar, A" uniqKey="Kumar A">A. Kumar</name></author><author><name sortKey="Bartenschlager, R" uniqKey="Bartenschlager R">R. Bartenschlager</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Adams, S C" uniqKey="Adams S">S. C. Adams</name></author><author><name sortKey="Broom, A K" uniqKey="Broom A">A. K. Broom</name></author><author><name sortKey="Sammels, L M" uniqKey="Sammels L">L. M. Sammels</name></author><author><name sortKey="Hartnett, A C" uniqKey="Hartnett A">A. C. Hartnett</name></author><author><name sortKey="Howard, M J" uniqKey="Howard M">M. J. Howard</name></author><author><name sortKey="Coelen, R J" uniqKey="Coelen R">R. J. Coelen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Akoua Koffi, C" uniqKey="Akoua Koffi C">C. Akoua-Koffi</name></author><author><name sortKey="Diarrassouba, S" uniqKey="Diarrassouba S">S. Diarrassouba</name></author><author><name sortKey="Benie, V B" uniqKey="Benie V">V. B. Benie</name></author><author><name sortKey="Ngbichi, J M" uniqKey="Ngbichi J">J. M. Ngbichi</name></author><author><name sortKey="Bozoua, T" uniqKey="Bozoua T">T. Bozoua</name></author><author><name sortKey="Bosson, A" uniqKey="Bosson A">A. Bosson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alera, M T" uniqKey="Alera M">M. T. Alera</name></author><author><name sortKey="Hermann, L" uniqKey="Hermann L">L. Hermann</name></author><author><name sortKey="Tac An, I A" uniqKey="Tac An I">I. A. Tac-An</name></author><author><name sortKey="Klungthong, C" uniqKey="Klungthong C">C. Klungthong</name></author><author><name sortKey="Rutvisuttinunt, W" uniqKey="Rutvisuttinunt W">W. Rutvisuttinunt</name></author><author><name sortKey="Manasatienkij, W" uniqKey="Manasatienkij W">W. Manasatienkij</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Althouse, B M" uniqKey="Althouse B">B. M. Althouse</name></author><author><name sortKey="Hanley, K A" uniqKey="Hanley K">K. A. Hanley</name></author><author><name sortKey="Diallo, M" uniqKey="Diallo M">M. Diallo</name></author><author><name sortKey="Sall, A A" uniqKey="Sall A">A. A. Sall</name></author><author><name sortKey="Ba, Y" uniqKey="Ba Y">Y. Ba</name></author><author><name sortKey="Faye, O" uniqKey="Faye O">O. Faye</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Apte Sengupta, S" uniqKey="Apte Sengupta S">S. Apte-Sengupta</name></author><author><name sortKey="Sirohi, D" uniqKey="Sirohi D">D. Sirohi</name></author><author><name sortKey="Kuhn, R J" uniqKey="Kuhn R">R. J. Kuhn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aubry, M" uniqKey="Aubry M">M. Aubry</name></author><author><name sortKey="Richard, V" uniqKey="Richard V">V. Richard</name></author><author><name sortKey="Green, J" uniqKey="Green J">J. Green</name></author><author><name sortKey="Broult, J" uniqKey="Broult J">J. Broult</name></author><author><name sortKey="Musso, D" uniqKey="Musso D">D. Musso</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aubry, M" uniqKey="Aubry M">M. Aubry</name></author><author><name sortKey="Teissier, A" uniqKey="Teissier A">A. Teissier</name></author><author><name sortKey="Roche, C" uniqKey="Roche C">C. Roche</name></author><author><name sortKey="Richard, V" uniqKey="Richard V">V. Richard</name></author><author><name sortKey="Yan, A S" uniqKey="Yan A">A. S. Yan</name></author><author><name sortKey="Zisou, K" uniqKey="Zisou K">K. Zisou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Balm, M N" uniqKey="Balm M">M. N. Balm</name></author><author><name sortKey="Lee, C K" uniqKey="Lee C">C. K. Lee</name></author><author><name sortKey="Lee, H K" uniqKey="Lee H">H. K. Lee</name></author><author><name sortKey="Chiu, L" uniqKey="Chiu L">L. Chiu</name></author><author><name sortKey="Koay, E S" uniqKey="Koay E">E. S. Koay</name></author><author><name sortKey="Tang, J W" uniqKey="Tang J">J. W. Tang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baronti, C" uniqKey="Baronti C">C. Baronti</name></author><author><name sortKey="Piorkowski, G" uniqKey="Piorkowski G">G. Piorkowski</name></author><author><name sortKey="Charrel, R N" uniqKey="Charrel R">R. N. Charrel</name></author><author><name sortKey="Boubis, L" uniqKey="Boubis L">L. Boubis</name></author><author><name sortKey="Leparc Goffart, I" uniqKey="Leparc Goffart I">I. Leparc-Goffart</name></author><author><name sortKey="De Lamballerie, X" uniqKey="De Lamballerie X">X. de Lamballerie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bearcroft, W G" uniqKey="Bearcroft W">W. G. Bearcroft</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bell, T M" uniqKey="Bell T">T. M. Bell</name></author><author><name sortKey="Field, E J" uniqKey="Field E">E. J. Field</name></author><author><name sortKey="Narang, H K" uniqKey="Narang H">H. K. Narang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ben Nathan, D" uniqKey="Ben Nathan D">D. Ben-Nathan</name></author><author><name sortKey="Lustig, S" uniqKey="Lustig S">S. Lustig</name></author><author><name sortKey="Tam, G" uniqKey="Tam G">G. Tam</name></author><author><name sortKey="Robinzon, S" uniqKey="Robinzon S">S. Robinzon</name></author><author><name sortKey="Segal, S" uniqKey="Segal S">S. Segal</name></author><author><name sortKey="Rager Zisman, B" uniqKey="Rager Zisman B">B. Rager-Zisman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Berthet, F X" uniqKey="Berthet F">F. X. Berthet</name></author><author><name sortKey="Zeller, H G" uniqKey="Zeller H">H. G. Zeller</name></author><author><name sortKey="Drouet, M T" uniqKey="Drouet M">M. T. Drouet</name></author><author><name sortKey="Rauzier, J" uniqKey="Rauzier J">J. Rauzier</name></author><author><name sortKey="Digoutte, J P" uniqKey="Digoutte J">J. P. Digoutte</name></author><author><name sortKey="Deubel, V" uniqKey="Deubel V">V. Deubel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Berthet, N" uniqKey="Berthet N">N. Berthet</name></author><author><name sortKey="Nakoune, E" uniqKey="Nakoune E">E. Nakoune</name></author><author><name sortKey="Kamgang, B" uniqKey="Kamgang B">B. Kamgang</name></author><author><name sortKey="Selekon, B" uniqKey="Selekon B">B. Selekon</name></author><author><name sortKey="Descorps Declere, S" uniqKey="Descorps Declere S">S. Descorps-Declere</name></author><author><name sortKey="Gessain, A" uniqKey="Gessain A">A. Gessain</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Besnard, M" uniqKey="Besnard M">M. Besnard</name></author><author><name sortKey="Lastere, S" uniqKey="Lastere S">S. Lastere</name></author><author><name sortKey="Teissier, A" uniqKey="Teissier A">A. Teissier</name></author><author><name sortKey="Cao Lormeau, V" uniqKey="Cao Lormeau V">V. Cao-Lormeau</name></author><author><name sortKey="Musso, D" uniqKey="Musso D">D. Musso</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Blazquez, A B" uniqKey="Blazquez A">A. B. Blazquez</name></author><author><name sortKey="Escribano Romero, E" uniqKey="Escribano Romero E">E. Escribano-Romero</name></author><author><name sortKey="Martin Acebes, M A" uniqKey="Martin Acebes M">M. A. Martin-Acebes</name></author><author><name sortKey="Petrovic, T" uniqKey="Petrovic T">T. Petrovic</name></author><author><name sortKey="Saiz, J C" uniqKey="Saiz J">J. C. Saiz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Blazquez, A B" uniqKey="Blazquez A">A. B. Blazquez</name></author><author><name sortKey="Escribano Romero, E" uniqKey="Escribano Romero E">E. Escribano-Romero</name></author><author><name sortKey="Merino Ramos, T" uniqKey="Merino Ramos T">T. Merino-Ramos</name></author><author><name sortKey="Saiz, J C" uniqKey="Saiz J">J. C. Saiz</name></author><author><name sortKey="Martin Acebes, M A" uniqKey="Martin Acebes M">M. A. Martin-Acebes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Buathong, R" uniqKey="Buathong R">R. Buathong</name></author><author><name sortKey="Hermann, L" uniqKey="Hermann L">L. Hermann</name></author><author><name sortKey="Thaisomboonsuk, B" uniqKey="Thaisomboonsuk B">B. Thaisomboonsuk</name></author><author><name sortKey="Rutvisuttinunt, W" uniqKey="Rutvisuttinunt W">W. Rutvisuttinunt</name></author><author><name sortKey="Klungthong, C" uniqKey="Klungthong C">C. Klungthong</name></author><author><name sortKey="Chinnawirotpisan, P" uniqKey="Chinnawirotpisan P">P. Chinnawirotpisan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Buckley, A" uniqKey="Buckley A">A. Buckley</name></author><author><name sortKey="Gould, E A" uniqKey="Gould E">E. A. Gould</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Butler, D" uniqKey="Butler D">D. Butler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Calvet, G A" uniqKey="Calvet G">G. A. Calvet</name></author><author><name sortKey="Filippis, A M" uniqKey="Filippis A">A. M. Filippis</name></author><author><name sortKey="Mendonca, M C" uniqKey="Mendonca M">M. C. Mendonca</name></author><author><name sortKey="Sequeira, P C" uniqKey="Sequeira P">P. C. Sequeira</name></author><author><name sortKey="Siqueira, A M" uniqKey="Siqueira A">A. M. Siqueira</name></author><author><name sortKey="Veloso, V G" uniqKey="Veloso V">V. G. Veloso</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Campos, G S" uniqKey="Campos G">G. S. Campos</name></author><author><name sortKey="Bandeira, A C" uniqKey="Bandeira A">A. C. Bandeira</name></author><author><name sortKey="Sardi, S I" uniqKey="Sardi S">S. I. Sardi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cao Lormeau, V M" uniqKey="Cao Lormeau V">V. M. Cao-Lormeau</name></author><author><name sortKey="Roche, C" uniqKey="Roche C">C. Roche</name></author><author><name sortKey="Teissier, A" uniqKey="Teissier A">A. Teissier</name></author><author><name sortKey="Robin, E" uniqKey="Robin E">E. Robin</name></author><author><name sortKey="Berry, A L" uniqKey="Berry A">A. L. Berry</name></author><author><name sortKey="Mallet, H P" uniqKey="Mallet H">H. P. Mallet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Caron, M" uniqKey="Caron M">M. Caron</name></author><author><name sortKey="Paupy, C" uniqKey="Paupy C">C. Paupy</name></author><author><name sortKey="Grard, G" uniqKey="Grard G">G. Grard</name></author><author><name sortKey="Becquart, P" uniqKey="Becquart P">P. Becquart</name></author><author><name sortKey="Mombo, I" uniqKey="Mombo I">I. Mombo</name></author><author><name sortKey="Nso, B B" uniqKey="Nso B">B. B. Nso</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Daffis, S" uniqKey="Daffis S">S. Daffis</name></author><author><name sortKey="Szretter, K J" uniqKey="Szretter K">K. J. Szretter</name></author><author><name sortKey="Schriewer, J" uniqKey="Schriewer J">J. Schriewer</name></author><author><name sortKey="Li, J" uniqKey="Li J">J. Li</name></author><author><name sortKey="Youn, S" uniqKey="Youn S">S. Youn</name></author><author><name sortKey="Errett, J" uniqKey="Errett J">J. Errett</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Darwish, M A" uniqKey="Darwish M">M. A. Darwish</name></author><author><name sortKey="Hoogstraal, H" uniqKey="Hoogstraal H">H. Hoogstraal</name></author><author><name sortKey="Roberts, T J" uniqKey="Roberts T">T. J. Roberts</name></author><author><name sortKey="Ahmed, I P" uniqKey="Ahmed I">I. P. Ahmed</name></author><author><name sortKey="Omar, F" uniqKey="Omar F">F. Omar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dauphin, G" uniqKey="Dauphin G">G. Dauphin</name></author><author><name sortKey="Zientara, S" uniqKey="Zientara S">S. Zientara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Paula Freitas, B" uniqKey="De Paula Freitas B">B. de Paula Freitas</name></author><author><name sortKey="De Oliveira Dias, J R" uniqKey="De Oliveira Dias J">J. R. de Oliveira Dias</name></author><author><name sortKey="Prazeres, J" uniqKey="Prazeres J">J. Prazeres</name></author><author><name sortKey="Sacramento, G A" uniqKey="Sacramento G">G. A. Sacramento</name></author><author><name sortKey="Ko, A I" uniqKey="Ko A">A. I. Ko</name></author><author><name sortKey="Maia, M" uniqKey="Maia M">M. Maia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Delatte, H" uniqKey="Delatte H">H. Delatte</name></author><author><name sortKey="Desvars, A" uniqKey="Desvars A">A. Desvars</name></author><author><name sortKey="Bouetard, A" uniqKey="Bouetard A">A. Bouetard</name></author><author><name sortKey="Bord, S" uniqKey="Bord S">S. Bord</name></author><author><name sortKey="Gimonneau, G" uniqKey="Gimonneau G">G. Gimonneau</name></author><author><name sortKey="Vourc, G" uniqKey="Vourc G">G. Vourc’h</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Delisle, E" uniqKey="Delisle E">E. Delisle</name></author><author><name sortKey="Rousseau, C" uniqKey="Rousseau C">C. Rousseau</name></author><author><name sortKey="Broche, B" uniqKey="Broche B">B. Broche</name></author><author><name sortKey="Leparc Goffart, I" uniqKey="Leparc Goffart I">I. Leparc-Goffart</name></author><author><name sortKey="L Mbert, G" uniqKey="L Mbert G">G. L’Ambert</name></author><author><name sortKey="Cochet, A" uniqKey="Cochet A">A. Cochet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dereeper, A" uniqKey="Dereeper A">A. Dereeper</name></author><author><name sortKey="Guignon, V" uniqKey="Guignon V">V. Guignon</name></author><author><name sortKey="Blanc, G" uniqKey="Blanc G">G. Blanc</name></author><author><name sortKey="Audic, S" uniqKey="Audic S">S. Audic</name></author><author><name sortKey="Buffet, S" uniqKey="Buffet S">S. Buffet</name></author><author><name sortKey="Chevenet, F" uniqKey="Chevenet F">F. Chevenet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Diagne, C T" uniqKey="Diagne C">C. T. Diagne</name></author><author><name sortKey="Diallo, D" uniqKey="Diallo D">D. Diallo</name></author><author><name sortKey="Faye, O" uniqKey="Faye O">O. Faye</name></author><author><name sortKey="Ba, Y" uniqKey="Ba Y">Y. Ba</name></author><author><name sortKey="Gaye, A" uniqKey="Gaye A">A. Gaye</name></author><author><name sortKey="Dia, I" uniqKey="Dia I">I. Dia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Diallo, D" uniqKey="Diallo D">D. Diallo</name></author><author><name sortKey="Sall, A A" uniqKey="Sall A">A. A. Sall</name></author><author><name sortKey="Diagne, C T" uniqKey="Diagne C">C. T. Diagne</name></author><author><name sortKey="Faye, O" uniqKey="Faye O">O. Faye</name></author><author><name sortKey="Ba, Y" uniqKey="Ba Y">Y. Ba</name></author><author><name sortKey="Hanley, K A" uniqKey="Hanley K">K. A. Hanley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dick, G W" uniqKey="Dick G">G. W. Dick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dick, G W" uniqKey="Dick G">G. W. Dick</name></author><author><name sortKey="Kitchen, S F" uniqKey="Kitchen S">S. F. Kitchen</name></author><author><name sortKey="Haddow, A J" uniqKey="Haddow A">A. J. Haddow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dominguez Moreno, R" uniqKey="Dominguez Moreno R">R. Dominguez-Moreno</name></author><author><name sortKey="Tolosa Tort, P" uniqKey="Tolosa Tort P">P. Tolosa-Tort</name></author><author><name sortKey="Patino Tamez, A" uniqKey="Patino Tamez A">A. Patino-Tamez</name></author><author><name sortKey="Quintero Bauman, A" uniqKey="Quintero Bauman A">A. Quintero-Bauman</name></author><author><name sortKey="Collado Frias, D K" uniqKey="Collado Frias D">D. K. Collado-Frias</name></author><author><name sortKey="Miranda Rodriguez, M G" uniqKey="Miranda Rodriguez M">M. G. Miranda-Rodriguez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dong, H" uniqKey="Dong H">H. Dong</name></author><author><name sortKey="Fink, K" uniqKey="Fink K">K. Fink</name></author><author><name sortKey="Zust, R" uniqKey="Zust R">R. Zust</name></author><author><name sortKey="Lim, S P" uniqKey="Lim S">S. P. Lim</name></author><author><name sortKey="Qin, C F" uniqKey="Qin C">C. F. Qin</name></author><author><name sortKey="Shi, P Y" uniqKey="Shi P">P. Y. Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Duffy, M R" uniqKey="Duffy M">M. R. Duffy</name></author><author><name sortKey="Chen, T H" uniqKey="Chen T">T. H. Chen</name></author><author><name sortKey="Hancock, W T" uniqKey="Hancock W">W. T. Hancock</name></author><author><name sortKey="Powers, A M" uniqKey="Powers A">A. M. Powers</name></author><author><name sortKey="Kool, J L" uniqKey="Kool J">J. L. Kool</name></author><author><name sortKey="Lanciotti, R S" uniqKey="Lanciotti R">R. S. Lanciotti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dupont Rouzeyrol, M" uniqKey="Dupont Rouzeyrol M">M. Dupont-Rouzeyrol</name></author><author><name sortKey="O Onnor, O" uniqKey="O Onnor O">O. O’Connor</name></author><author><name sortKey="Calvez, E" uniqKey="Calvez E">E. Calvez</name></author><author><name sortKey="Daures, M" uniqKey="Daures M">M. Daures</name></author><author><name sortKey="John, M" uniqKey="John M">M. John</name></author><author><name sortKey="Grangeon, J P" uniqKey="Grangeon J">J. P. Grangeon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dyer, O" uniqKey="Dyer O">O. Dyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Engle, M J" uniqKey="Engle M">M. J. Engle</name></author><author><name sortKey="Diamond, M S" uniqKey="Diamond M">M. S. Diamond</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fagbami, A H" uniqKey="Fagbami A">A. H. Fagbami</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fauci, A S" uniqKey="Fauci A">A. S. Fauci</name></author><author><name sortKey="Morens, D M" uniqKey="Morens D">D. M. Morens</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Faye, O" uniqKey="Faye O">O. Faye</name></author><author><name sortKey="Diallo, D" uniqKey="Diallo D">D. Diallo</name></author><author><name sortKey="Diallo, M" uniqKey="Diallo M">M. Diallo</name></author><author><name sortKey="Weidmann, M" uniqKey="Weidmann M">M. Weidmann</name></author><author><name sortKey="Sall, A A" uniqKey="Sall A">A. A. Sall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Faye, O" uniqKey="Faye O">O. Faye</name></author><author><name sortKey="Dupressoir, A" uniqKey="Dupressoir A">A. Dupressoir</name></author><author><name sortKey="Weidmann, M" uniqKey="Weidmann M">M. Weidmann</name></author><author><name sortKey="Ndiaye, M" uniqKey="Ndiaye M">M. Ndiaye</name></author><author><name sortKey="Alpha Sall, A" uniqKey="Alpha Sall A">A. Alpha Sall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Faye, O" uniqKey="Faye O">O. Faye</name></author><author><name sortKey="Freire, C C" uniqKey="Freire C">C. C. Freire</name></author><author><name sortKey="Iamarino, A" uniqKey="Iamarino A">A. Iamarino</name></author><author><name sortKey="De Oliveira, J V" uniqKey="De Oliveira J">J. V. de Oliveira</name></author><author><name sortKey="Diallo, M" uniqKey="Diallo M">M. Diallo</name></author><author><name sortKey="Zanotto, P M" uniqKey="Zanotto P">P. M. Zanotto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Filipe, A R" uniqKey="Filipe A">A. R. Filipe</name></author><author><name sortKey="Martins, C M" uniqKey="Martins C">C. M. Martins</name></author><author><name sortKey="Rocha, H" uniqKey="Rocha H">H. Rocha</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Foy, B D" uniqKey="Foy B">B. D. Foy</name></author><author><name sortKey="Kobylinski, K C" uniqKey="Kobylinski K">K. C. Kobylinski</name></author><author><name sortKey="Chilson Foy, J L" uniqKey="Chilson Foy J">J. L. Chilson Foy</name></author><author><name sortKey="Blitvich, B J" uniqKey="Blitvich B">B. J. Blitvich</name></author><author><name sortKey="Travassos Da Rosa, A" uniqKey="Travassos Da Rosa A">A. Travassos da Rosa</name></author><author><name sortKey="Haddow, A D" uniqKey="Haddow A">A. D. Haddow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Freire, C C D M" uniqKey="Freire C">C. C. D. M. Freire</name></author><author><name sortKey="Iamarino, A" uniqKey="Iamarino A">A. Iamarino</name></author><author><name sortKey="Neto, D F D L" uniqKey="Neto D">D. F. D. L. Neto</name></author><author><name sortKey="Sall, A A" uniqKey="Sall A">A. A. Sall</name></author><author><name sortKey="Zanotto, P M D A" uniqKey="Zanotto P">P. M. D. A. Zanotto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gatherer, D" uniqKey="Gatherer D">D. Gatherer</name></author><author><name sortKey="Kohl, A" uniqKey="Kohl A">A. Kohl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Geser, A" uniqKey="Geser A">A. Geser</name></author><author><name sortKey="Henderson, B E" uniqKey="Henderson B">B. E. Henderson</name></author><author><name sortKey="Christensen, S" uniqKey="Christensen S">S. Christensen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gjenero Margan, I" uniqKey="Gjenero Margan I">I. Gjenero-Margan</name></author><author><name sortKey="Aleraj, B" uniqKey="Aleraj B">B. Aleraj</name></author><author><name sortKey="Krajcar, D" uniqKey="Krajcar D">D. Krajcar</name></author><author><name sortKey="Lesnikar, V" uniqKey="Lesnikar V">V. Lesnikar</name></author><author><name sortKey="Klobucar, A" uniqKey="Klobucar A">A. Klobucar</name></author><author><name sortKey="Pem Novosel, I" uniqKey="Pem Novosel I">I. Pem-Novosel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gollins, S W" uniqKey="Gollins S">S. W. Gollins</name></author><author><name sortKey="Porterfield, J S" uniqKey="Porterfield J">J. S. Porterfield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gould, E A" uniqKey="Gould E">E. A. Gould</name></author><author><name sortKey="Solomon, T" uniqKey="Solomon T">T. Solomon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gourinat, A C" uniqKey="Gourinat A">A. C. Gourinat</name></author><author><name sortKey="O Onnor, O" uniqKey="O Onnor O">O. O’Connor</name></author><author><name sortKey="Calvez, E" uniqKey="Calvez E">E. Calvez</name></author><author><name sortKey="Goarant, C" uniqKey="Goarant C">C. Goarant</name></author><author><name sortKey="Dupont Rouzeyrol, M" uniqKey="Dupont Rouzeyrol M">M. Dupont-Rouzeyrol</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grard, G" uniqKey="Grard G">G. Grard</name></author><author><name sortKey="Caron, M" uniqKey="Caron M">M. Caron</name></author><author><name sortKey="Mombo, I M" uniqKey="Mombo I">I. M. Mombo</name></author><author><name sortKey="Nkoghe, D" uniqKey="Nkoghe D">D. Nkoghe</name></author><author><name sortKey="Mboui Ondo, S" uniqKey="Mboui Ondo S">S. Mboui Ondo</name></author><author><name sortKey="Jiolle, D" uniqKey="Jiolle D">D. Jiolle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haddow, A D" uniqKey="Haddow A">A. D. Haddow</name></author><author><name sortKey="Schuh, A J" uniqKey="Schuh A">A. J. Schuh</name></author><author><name sortKey="Yasuda, C Y" uniqKey="Yasuda C">C. Y. Yasuda</name></author><author><name sortKey="Kasper, M R" uniqKey="Kasper M">M. R. Kasper</name></author><author><name sortKey="Heang, V" uniqKey="Heang V">V. Heang</name></author><author><name sortKey="Huy, R" uniqKey="Huy R">R. Huy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haddow, A J" uniqKey="Haddow A">A. J. Haddow</name></author><author><name sortKey="Dick, G W" uniqKey="Dick G">G. W. Dick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haddow, A J" uniqKey="Haddow A">A. J. Haddow</name></author><author><name sortKey="Williams, M C" uniqKey="Williams M">M. C. Williams</name></author><author><name sortKey="Woodall, J P" uniqKey="Woodall J">J. P. Woodall</name></author><author><name sortKey="Simpson, D I" uniqKey="Simpson D">D. I. Simpson</name></author><author><name sortKey="Goma, L K" uniqKey="Goma L">L. K. Goma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hamel, R" uniqKey="Hamel R">R. Hamel</name></author><author><name sortKey="Dejarnac, O" uniqKey="Dejarnac O">O. Dejarnac</name></author><author><name sortKey="Wichit, S" uniqKey="Wichit S">S. Wichit</name></author><author><name sortKey="Ekchariyawat, P" uniqKey="Ekchariyawat P">P. Ekchariyawat</name></author><author><name sortKey="Neyret, A" uniqKey="Neyret A">A. Neyret</name></author><author><name sortKey="Luplertlop, N" uniqKey="Luplertlop N">N. Luplertlop</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hammon, W M" uniqKey="Hammon W">W. M. Hammon</name></author><author><name sortKey="Schrack, W D" uniqKey="Schrack W">W. D. Schrack</name></author><author><name sortKey="Sather, G E" uniqKey="Sather G">G. E. Sather</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Heang, V" uniqKey="Heang V">V. Heang</name></author><author><name sortKey="Yasuda, C Y" uniqKey="Yasuda C">C. Y. Yasuda</name></author><author><name sortKey="Sovann, L" uniqKey="Sovann L">L. Sovann</name></author><author><name sortKey="Haddow, A D" uniqKey="Haddow A">A. D. Haddow</name></author><author><name sortKey="Travassos Da Rosa, A P" uniqKey="Travassos Da Rosa A">A. P. Travassos da Rosa</name></author><author><name sortKey="Tesh, R B" uniqKey="Tesh R">R. B. Tesh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Higgs, S" uniqKey="Higgs S">S. Higgs</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Holmes, E C" uniqKey="Holmes E">E. C. Holmes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ishikawa, T" uniqKey="Ishikawa T">T. Ishikawa</name></author><author><name sortKey="Yamanaka, A" uniqKey="Yamanaka A">A. Yamanaka</name></author><author><name sortKey="Konishi, E" uniqKey="Konishi E">E. Konishi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jan, C" uniqKey="Jan C">C. Jan</name></author><author><name sortKey="Languillat, G" uniqKey="Languillat G">G. Languillat</name></author><author><name sortKey="Renaudet, J" uniqKey="Renaudet J">J. Renaudet</name></author><author><name sortKey="Robin, Y" uniqKey="Robin Y">Y. Robin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Johnson, B K" uniqKey="Johnson B">B. K. Johnson</name></author><author><name sortKey="Chanas, A C" uniqKey="Chanas A">A. C. Chanas</name></author><author><name sortKey="Shockley, P" uniqKey="Shockley P">P. Shockley</name></author><author><name sortKey="Squires, E J" uniqKey="Squires E">E. J. Squires</name></author><author><name sortKey="Gardner, P" uniqKey="Gardner P">P. Gardner</name></author><author><name sortKey="Wallace, C" uniqKey="Wallace C">C. Wallace</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kraemer, M U" uniqKey="Kraemer M">M. U. Kraemer</name></author><author><name sortKey="Sinka, M E" uniqKey="Sinka M">M. E. Sinka</name></author><author><name sortKey="Duda, K A" uniqKey="Duda K">K. A. Duda</name></author><author><name sortKey="Mylne, A Q" uniqKey="Mylne A">A. Q. Mylne</name></author><author><name sortKey="Shearer, F M" uniqKey="Shearer F">F. M. Shearer</name></author><author><name sortKey="Barker, C M" uniqKey="Barker C">C. M. Barker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuhn, R J" uniqKey="Kuhn R">R. J. Kuhn</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W. Zhang</name></author><author><name sortKey="Rossmann, M G" uniqKey="Rossmann M">M. G. Rossmann</name></author><author><name sortKey="Pletnev, S V" uniqKey="Pletnev S">S. V. Pletnev</name></author><author><name sortKey="Corver, J" uniqKey="Corver J">J. Corver</name></author><author><name sortKey="Lenches, E" uniqKey="Lenches E">E. Lenches</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuno, G" uniqKey="Kuno G">G. Kuno</name></author><author><name sortKey="Chang, G J" uniqKey="Chang G">G. J. Chang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuno, G" uniqKey="Kuno G">G. Kuno</name></author><author><name sortKey="Chang, G J" uniqKey="Chang G">G. J. Chang</name></author><author><name sortKey="Tsuchiya, K R" uniqKey="Tsuchiya K">K. R. Tsuchiya</name></author><author><name sortKey="Karabatsos, N" uniqKey="Karabatsos N">N. Karabatsos</name></author><author><name sortKey="Cropp, C B" uniqKey="Cropp C">C. B. Cropp</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kutsuna, S" uniqKey="Kutsuna S">S. Kutsuna</name></author><author><name sortKey="Kato, Y" uniqKey="Kato Y">Y. Kato</name></author><author><name sortKey="Takasaki, T" uniqKey="Takasaki T">T. Takasaki</name></author><author><name sortKey="Moi, M" uniqKey="Moi M">M. Moi</name></author><author><name sortKey="Kotaki, A" uniqKey="Kotaki A">A. Kotaki</name></author><author><name sortKey="Uemura, H" uniqKey="Uemura H">H. Uemura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kwong, J C" uniqKey="Kwong J">J. C. Kwong</name></author><author><name sortKey="Druce, J D" uniqKey="Druce J">J. D. Druce</name></author><author><name sortKey="Leder, K" uniqKey="Leder K">K. Leder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="La Ruche, G" uniqKey="La Ruche G">G. La Ruche</name></author><author><name sortKey="Souares, Y" uniqKey="Souares Y">Y. Souares</name></author><author><name sortKey="Armengaud, A" uniqKey="Armengaud A">A. Armengaud</name></author><author><name sortKey="Peloux Petiot, F" uniqKey="Peloux Petiot F">F. Peloux-Petiot</name></author><author><name sortKey="Delaunay, P" uniqKey="Delaunay P">P. Delaunay</name></author><author><name sortKey="Despres, P" uniqKey="Despres P">P. Despres</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lanciotti, R S" uniqKey="Lanciotti R">R. S. Lanciotti</name></author><author><name sortKey="Kosoy, O L" uniqKey="Kosoy O">O. L. Kosoy</name></author><author><name sortKey="Laven, J J" uniqKey="Laven J">J. J. Laven</name></author><author><name sortKey="Velez, J O" uniqKey="Velez J">J. O. Velez</name></author><author><name sortKey="Lambert, A J" uniqKey="Lambert A">A. J. Lambert</name></author><author><name sortKey="Johnson, A J" uniqKey="Johnson A">A. J. Johnson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, V H" uniqKey="Lee V">V. H. Lee</name></author><author><name sortKey="Moore, D L" uniqKey="Moore D">D. L. Moore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Leung, G H" uniqKey="Leung G">G. H. Leung</name></author><author><name sortKey="Baird, R W" uniqKey="Baird R">R. W. Baird</name></author><author><name sortKey="Druce, J" uniqKey="Druce J">J. Druce</name></author><author><name sortKey="Anstey, N M" uniqKey="Anstey N">N. M. Anstey</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, J" uniqKey="Li J">J. Li</name></author><author><name sortKey="Bhuvanakantham, R" uniqKey="Bhuvanakantham R">R. Bhuvanakantham</name></author><author><name sortKey="Howe, J" uniqKey="Howe J">J. Howe</name></author><author><name sortKey="Ng, M L" uniqKey="Ng M">M. L. Ng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, M I" uniqKey="Li M">M. I. Li</name></author><author><name sortKey="Wong, P S" uniqKey="Wong P">P. S. Wong</name></author><author><name sortKey="Ng, L C" uniqKey="Ng L">L. C. Ng</name></author><author><name sortKey="Tan, C H" uniqKey="Tan C">C. H. Tan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lok, S M" uniqKey="Lok S">S. M. Lok</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luo, D" uniqKey="Luo D">D. Luo</name></author><author><name sortKey="Vasudevan, S G" uniqKey="Vasudevan S">S. G. Vasudevan</name></author><author><name sortKey="Lescar, J" uniqKey="Lescar J">J. Lescar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Macnamara, F N" uniqKey="Macnamara F">F. N. Macnamara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marano, G" uniqKey="Marano G">G. Marano</name></author><author><name sortKey="Pupella, S" uniqKey="Pupella S">S. Pupella</name></author><author><name sortKey="Vaglio, S" uniqKey="Vaglio S">S. Vaglio</name></author><author><name sortKey="Liumbruno, G M" uniqKey="Liumbruno G">G. M. Liumbruno</name></author><author><name sortKey="Grazzini, G" uniqKey="Grazzini G">G. Grazzini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marchette, N J" uniqKey="Marchette N">N. J. Marchette</name></author><author><name sortKey="Garcia, R" uniqKey="Garcia R">R. Garcia</name></author><author><name sortKey="Rudnick, A" uniqKey="Rudnick A">A. Rudnick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marcondes, C B" uniqKey="Marcondes C">C. B. Marcondes</name></author><author><name sortKey="Ximenes, M F" uniqKey="Ximenes M">M. F. Ximenes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martin Acebes, M A" uniqKey="Martin Acebes M">M. A. Martin-Acebes</name></author><author><name sortKey="Blazquez, A B" uniqKey="Blazquez A">A. B. Blazquez</name></author><author><name sortKey="Jimenez De Oya, N" uniqKey="Jimenez De Oya N">N. Jimenez de Oya</name></author><author><name sortKey="Escribano Romero, E" uniqKey="Escribano Romero E">E. Escribano-Romero</name></author><author><name sortKey="Saiz, J C" uniqKey="Saiz J">J. C. Saiz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martin Acebes, M A" uniqKey="Martin Acebes M">M. A. Martin-Acebes</name></author><author><name sortKey="Saiz, J C" uniqKey="Saiz J">J. C. Saiz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martines, R B" uniqKey="Martines R">R. B. Martines</name></author><author><name sortKey="Bhatnagar, J" uniqKey="Bhatnagar J">J. Bhatnagar</name></author><author><name sortKey="Keating, M K" uniqKey="Keating M">M. K. Keating</name></author><author><name sortKey="Silva Flannery, L" uniqKey="Silva Flannery L">L. Silva-Flannery</name></author><author><name sortKey="Muehlenbachs, A" uniqKey="Muehlenbachs A">A. Muehlenbachs</name></author><author><name sortKey="Gary, J" uniqKey="Gary J">J. Gary</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mccarthy, M" uniqKey="Mccarthy M">M. McCarthy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mccrae, A W" uniqKey="Mccrae A">A. W. McCrae</name></author><author><name sortKey="Kirya, B G" uniqKey="Kirya B">B. G. Kirya</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Medlock, J M" uniqKey="Medlock J">J. M. Medlock</name></author><author><name sortKey="Hansford, K M" uniqKey="Hansford K">K. M. Hansford</name></author><author><name sortKey="Schaffner, F" uniqKey="Schaffner F">F. Schaffner</name></author><author><name sortKey="Versteirt, V" uniqKey="Versteirt V">V. Versteirt</name></author><author><name sortKey="Hendrickx, G" uniqKey="Hendrickx G">G. Hendrickx</name></author><author><name sortKey="Zeller, H" uniqKey="Zeller H">H. Zeller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miorin, L" uniqKey="Miorin L">L. Miorin</name></author><author><name sortKey="Romero Brey, I" uniqKey="Romero Brey I">I. Romero-Brey</name></author><author><name sortKey="Maiuri, P" uniqKey="Maiuri P">P. Maiuri</name></author><author><name sortKey="Hoppe, S" uniqKey="Hoppe S">S. Hoppe</name></author><author><name sortKey="Krijnse Locker, J" uniqKey="Krijnse Locker J">J. Krijnse-Locker</name></author><author><name sortKey="Bartenschlager, R" uniqKey="Bartenschlager R">R. Bartenschlager</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mlakar, J" uniqKey="Mlakar J">J. Mlakar</name></author><author><name sortKey="Korva, M" uniqKey="Korva M">M. Korva</name></author><author><name sortKey="Tul, N" uniqKey="Tul N">N. Tul</name></author><author><name sortKey="Popovic, M" uniqKey="Popovic M">M. Popovic</name></author><author><name sortKey="Poljsak Prijatelj, M" uniqKey="Poljsak Prijatelj M">M. Poljsak-Prijatelj</name></author><author><name sortKey="Mraz, J" uniqKey="Mraz J">J. Mraz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Monath, T P" uniqKey="Monath T">T. P. Monath</name></author><author><name sortKey="Vasconcelos, P F" uniqKey="Vasconcelos P">P. F. Vasconcelos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Monath, T P" uniqKey="Monath T">T. P. Monath</name></author><author><name sortKey="Wilson, D C" uniqKey="Wilson D">D. C. Wilson</name></author><author><name sortKey="Lee, V H" uniqKey="Lee V">V. H. Lee</name></author><author><name sortKey="Stroh, G" uniqKey="Stroh G">G. Stroh</name></author><author><name sortKey="Kuteyi, K" uniqKey="Kuteyi K">K. Kuteyi</name></author><author><name sortKey="Smith, E A" uniqKey="Smith E">E. A. Smith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Monlun, E" uniqKey="Monlun E">E. Monlun</name></author><author><name sortKey="Zeller, H" uniqKey="Zeller H">H. Zeller</name></author><author><name sortKey="Le Guenno, B" uniqKey="Le Guenno B">B. Le Guenno</name></author><author><name sortKey="Traore Lamizana, M" uniqKey="Traore Lamizana M">M. Traore-Lamizana</name></author><author><name sortKey="Hervy, J P" uniqKey="Hervy J">J. P. Hervy</name></author><author><name sortKey="Adam, F" uniqKey="Adam F">F. Adam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mukhopadhyay, S" uniqKey="Mukhopadhyay S">S. Mukhopadhyay</name></author><author><name sortKey="Kim, B S" uniqKey="Kim B">B. S. Kim</name></author><author><name sortKey="Chipman, P R" uniqKey="Chipman P">P. R. Chipman</name></author><author><name sortKey="Rossmann, M G" uniqKey="Rossmann M">M. G. Rossmann</name></author><author><name sortKey="Kuhn, R J" uniqKey="Kuhn R">R. J. Kuhn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mukhopadhyay, S" uniqKey="Mukhopadhyay S">S. Mukhopadhyay</name></author><author><name sortKey="Kuhn, R J" uniqKey="Kuhn R">R. J. Kuhn</name></author><author><name sortKey="Rossmann, M G" uniqKey="Rossmann M">M. G. Rossmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Musso, D" uniqKey="Musso D">D. Musso</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Musso, D" uniqKey="Musso D">D. Musso</name></author><author><name sortKey="Nhan, T" uniqKey="Nhan T">T. Nhan</name></author><author><name sortKey="Robin, E" uniqKey="Robin E">E. Robin</name></author><author><name sortKey="Roche, C" uniqKey="Roche C">C. Roche</name></author><author><name sortKey="Bierlaire, D" uniqKey="Bierlaire D">D. Bierlaire</name></author><author><name sortKey="Zisou, K" uniqKey="Zisou K">K. Zisou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Musso, D" uniqKey="Musso D">D. Musso</name></author><author><name sortKey="Nilles, E J" uniqKey="Nilles E">E. J. Nilles</name></author><author><name sortKey="Cao Lormeau, V M" uniqKey="Cao Lormeau V">V. M. Cao-Lormeau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Musso, D" uniqKey="Musso D">D. Musso</name></author><author><name sortKey="Roche, C" uniqKey="Roche C">C. Roche</name></author><author><name sortKey="Nhan, T X" uniqKey="Nhan T">T. X. Nhan</name></author><author><name sortKey="Robin, E" uniqKey="Robin E">E. Robin</name></author><author><name sortKey="Teissier, A" uniqKey="Teissier A">A. Teissier</name></author><author><name sortKey="Cao Lormeau, V M" uniqKey="Cao Lormeau V">V. M. Cao-Lormeau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Musso, D" uniqKey="Musso D">D. Musso</name></author><author><name sortKey="Roche, C" uniqKey="Roche C">C. Roche</name></author><author><name sortKey="Robin, E" uniqKey="Robin E">E. Robin</name></author><author><name sortKey="Nhan, T" uniqKey="Nhan T">T. Nhan</name></author><author><name sortKey="Teissier, A" uniqKey="Teissier A">A. Teissier</name></author><author><name sortKey="Cao Lormeau, V M" uniqKey="Cao Lormeau V">V. M. Cao-Lormeau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Noble, C G" uniqKey="Noble C">C. G. Noble</name></author><author><name sortKey="Shi, P Y" uniqKey="Shi P">P. Y. Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Oehler, E" uniqKey="Oehler E">E. Oehler</name></author><author><name sortKey="Watrin, L" uniqKey="Watrin L">L. Watrin</name></author><author><name sortKey="Larre, P" uniqKey="Larre P">P. Larre</name></author><author><name sortKey="Leparc Goffart, I" uniqKey="Leparc Goffart I">I. Leparc-Goffart</name></author><author><name sortKey="Lastere, S" uniqKey="Lastere S">S. Lastere</name></author><author><name sortKey="Valour, F" uniqKey="Valour F">F. Valour</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Oliveira Melo, A S" uniqKey="Oliveira Melo A">A. S. Oliveira Melo</name></author><author><name sortKey="Malinger, G" uniqKey="Malinger G">G. Malinger</name></author><author><name sortKey="Ximenes, R" uniqKey="Ximenes R">R. Ximenes</name></author><author><name sortKey="Szejnfeld, P O" uniqKey="Szejnfeld P">P. O. Szejnfeld</name></author><author><name sortKey="Alves Sampaio, S" uniqKey="Alves Sampaio S">S. Alves Sampaio</name></author><author><name sortKey="Bispo De Filippis, A M" uniqKey="Bispo De Filippis A">A. M. Bispo de Filippis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Olson, J G" uniqKey="Olson J">J. G. Olson</name></author><author><name sortKey="Ksiazek, T G" uniqKey="Ksiazek T">T. G. Ksiazek</name></author><author><name sortKey="Gubler, D J" uniqKey="Gubler D">D. J. Gubler</name></author><author><name sortKey="Lubis, S I" uniqKey="Lubis S">S. I. Lubis</name></author><author><name sortKey="Simanjuntak, G" uniqKey="Simanjuntak G">G. Simanjuntak</name></author><author><name sortKey="Lee, V H" uniqKey="Lee V">V. H. Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Olson, J G" uniqKey="Olson J">J. G. Olson</name></author><author><name sortKey="Ksiazek, T G" uniqKey="Ksiazek T">T. G. Ksiazek</name></author><author><name sortKey="Suhandiman" uniqKey="Suhandiman">Suhandiman</name></author><author><name sortKey="Triwibowo" uniqKey="Triwibowo">Triwibowo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paupy, C" uniqKey="Paupy C">C. Paupy</name></author><author><name sortKey="Delatte, H" uniqKey="Delatte H">H. Delatte</name></author><author><name sortKey="Bagny, L" uniqKey="Bagny L">L. Bagny</name></author><author><name sortKey="Corbel, V" uniqKey="Corbel V">V. Corbel</name></author><author><name sortKey="Fontenille, D" uniqKey="Fontenille D">D. Fontenille</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Petersen, E" uniqKey="Petersen E">E. Petersen</name></author><author><name sortKey="Wilson, M E" uniqKey="Wilson M">M. E. Wilson</name></author><author><name sortKey="Touch, S" uniqKey="Touch S">S. Touch</name></author><author><name sortKey="Mccloskey, B" uniqKey="Mccloskey B">B. McCloskey</name></author><author><name sortKey="Mwaba, P" uniqKey="Mwaba P">P. Mwaba</name></author><author><name sortKey="Bates, M" uniqKey="Bates M">M. Bates</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pierson, T C" uniqKey="Pierson T">T. C. Pierson</name></author><author><name sortKey="Diamond, M S" uniqKey="Diamond M">M. S. Diamond</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Plevka, P" uniqKey="Plevka P">P. Plevka</name></author><author><name sortKey="Battisti, A J" uniqKey="Battisti A">A. J. Battisti</name></author><author><name sortKey="Junjhon, J" uniqKey="Junjhon J">J. Junjhon</name></author><author><name sortKey="Winkler, D C" uniqKey="Winkler D">D. C. Winkler</name></author><author><name sortKey="Holdaway, H A" uniqKey="Holdaway H">H. A. Holdaway</name></author><author><name sortKey="Keelapang, P" uniqKey="Keelapang P">P. Keelapang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pond, W L" uniqKey="Pond W">W. L. Pond</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ponlawat, A" uniqKey="Ponlawat A">A. Ponlawat</name></author><author><name sortKey="Harrington, L C" uniqKey="Harrington L">L. C. Harrington</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Puccioni Sohler, M" uniqKey="Puccioni Sohler M">M. Puccioni-Sohler</name></author><author><name sortKey="Orsini, M" uniqKey="Orsini M">M. Orsini</name></author><author><name sortKey="Soares, C N" uniqKey="Soares C">C. N. Soares</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pupella, S" uniqKey="Pupella S">S. Pupella</name></author><author><name sortKey="Pisani, G" uniqKey="Pisani G">G. Pisani</name></author><author><name sortKey="Cristiano, K" uniqKey="Cristiano K">K. Cristiano</name></author><author><name sortKey="Catalano, L" uniqKey="Catalano L">L. Catalano</name></author><author><name sortKey="Grazzini, G" uniqKey="Grazzini G">G. Grazzini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reiter, P" uniqKey="Reiter P">P. Reiter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rezza, G" uniqKey="Rezza G">G. Rezza</name></author><author><name sortKey="Nicoletti, L" uniqKey="Nicoletti L">L. Nicoletti</name></author><author><name sortKey="Angelini, R" uniqKey="Angelini R">R. Angelini</name></author><author><name sortKey="Romi, R" uniqKey="Romi R">R. Romi</name></author><author><name sortKey="Finarelli, A C" uniqKey="Finarelli A">A. C. Finarelli</name></author><author><name sortKey="Panning, M" uniqKey="Panning M">M. Panning</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Robin, Y" uniqKey="Robin Y">Y. Robin</name></author><author><name sortKey="Mouchet, J" uniqKey="Mouchet J">J. Mouchet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roby, J A" uniqKey="Roby J">J. A. Roby</name></author><author><name sortKey="Setoh, Y X" uniqKey="Setoh Y">Y. X. Setoh</name></author><author><name sortKey="Hall, R A" uniqKey="Hall R">R. A. Hall</name></author><author><name sortKey="Khromykh, A A" uniqKey="Khromykh A">A. A. Khromykh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roehrig, J T" uniqKey="Roehrig J">J. T. Roehrig</name></author><author><name sortKey="Staudinger, L A" uniqKey="Staudinger L">L. A. Staudinger</name></author><author><name sortKey="Hunt, A R" uniqKey="Hunt A">A. R. Hunt</name></author><author><name sortKey="Mathews, J H" uniqKey="Mathews J">J. H. Mathews</name></author><author><name sortKey="Blair, C D" uniqKey="Blair C">C. D. Blair</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roth, A" uniqKey="Roth A">A. Roth</name></author><author><name sortKey="Mercier, A" uniqKey="Mercier A">A. Mercier</name></author><author><name sortKey="Lepers, C" uniqKey="Lepers C">C. Lepers</name></author><author><name sortKey="Hoy, D" uniqKey="Hoy D">D. Hoy</name></author><author><name sortKey="Duituturaga, S" uniqKey="Duituturaga S">S. Duituturaga</name></author><author><name sortKey="Benyon, E" uniqKey="Benyon E">E. Benyon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rubin, E J" uniqKey="Rubin E">E. J. Rubin</name></author><author><name sortKey="Greene, M F" uniqKey="Greene M">M. F. Greene</name></author><author><name sortKey="Baden, L R" uniqKey="Baden L">L. R. Baden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saluzzo, J F" uniqKey="Saluzzo J">J. F. Saluzzo</name></author><author><name sortKey="Gonzalez, J P" uniqKey="Gonzalez J">J. P. Gonzalez</name></author><author><name sortKey="Herve, J P" uniqKey="Herve J">J. P. Herve</name></author><author><name sortKey="Georges, A J" uniqKey="Georges A">A. J. Georges</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schuler Faccini, L" uniqKey="Schuler Faccini L">L. Schuler-Faccini</name></author><author><name sortKey="Ribeiro, E M" uniqKey="Ribeiro E">E. M. Ribeiro</name></author><author><name sortKey="Feitosa, I M" uniqKey="Feitosa I">I. M. Feitosa</name></author><author><name sortKey="Horovitz, D D" uniqKey="Horovitz D">D. D. Horovitz</name></author><author><name sortKey="Cavalcanti, D P" uniqKey="Cavalcanti D">D. P. Cavalcanti</name></author><author><name sortKey="Pessoa, A" uniqKey="Pessoa A">A. Pessoa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scott, T W" uniqKey="Scott T">T. W. Scott</name></author><author><name sortKey="Takken, W" uniqKey="Takken W">W. Takken</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shinohara, K" uniqKey="Shinohara K">K. Shinohara</name></author><author><name sortKey="Kutsuna, S" uniqKey="Kutsuna S">S. Kutsuna</name></author><author><name sortKey="Takasaki, T" uniqKey="Takasaki T">T. Takasaki</name></author><author><name sortKey="Moi, M L" uniqKey="Moi M">M. L. Moi</name></author><author><name sortKey="Ikeda, M" uniqKey="Ikeda M">M. Ikeda</name></author><author><name sortKey="Kotaki, A" uniqKey="Kotaki A">A. Kotaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shirato, K" uniqKey="Shirato K">K. Shirato</name></author><author><name sortKey="Miyoshi, H" uniqKey="Miyoshi H">H. Miyoshi</name></author><author><name sortKey="Goto, A" uniqKey="Goto A">A. Goto</name></author><author><name sortKey="Ako, Y" uniqKey="Ako Y">Y. Ako</name></author><author><name sortKey="Ueki, T" uniqKey="Ueki T">T. Ueki</name></author><author><name sortKey="Kariwa, H" uniqKey="Kariwa H">H. Kariwa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Simpson, D I" uniqKey="Simpson D">D. I. Simpson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smithburn, K C" uniqKey="Smithburn K">K. C. Smithburn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smithburn, K C" uniqKey="Smithburn K">K. C. Smithburn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smithburn, K C" uniqKey="Smithburn K">K. C. Smithburn</name></author><author><name sortKey="Kerr, J A" uniqKey="Kerr J">J. A. Kerr</name></author><author><name sortKey="Gatne, P B" uniqKey="Gatne P">P. B. Gatne</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Staples, J E" uniqKey="Staples J">J. E. Staples</name></author><author><name sortKey="Dziuban, E J" uniqKey="Dziuban E">E. J. Dziuban</name></author><author><name sortKey="Fischer, M" uniqKey="Fischer M">M. Fischer</name></author><author><name sortKey="Cragan, J D" uniqKey="Cragan J">J. D. Cragan</name></author><author><name sortKey="Rasmussen, S A" uniqKey="Rasmussen S">S. A. Rasmussen</name></author><author><name sortKey="Cannon, M J" uniqKey="Cannon M">M. J. Cannon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stiasny, K" uniqKey="Stiasny K">K. Stiasny</name></author><author><name sortKey="Fritz, R" uniqKey="Fritz R">R. Fritz</name></author><author><name sortKey="Pangerl, K" uniqKey="Pangerl K">K. Pangerl</name></author><author><name sortKey="Heinz, F X" uniqKey="Heinz F">F. X. Heinz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tappe, D" uniqKey="Tappe D">D. Tappe</name></author><author><name sortKey="Nachtigall, S" uniqKey="Nachtigall S">S. Nachtigall</name></author><author><name sortKey="Kapaun, A" uniqKey="Kapaun A">A. Kapaun</name></author><author><name sortKey="Schnitzler, P" uniqKey="Schnitzler P">P. Schnitzler</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name></author><author><name sortKey="Schmidt Chanasit, J" uniqKey="Schmidt Chanasit J">J. Schmidt-Chanasit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tappe, D" uniqKey="Tappe D">D. Tappe</name></author><author><name sortKey="Rissland, J" uniqKey="Rissland J">J. Rissland</name></author><author><name sortKey="Gabriel, M" uniqKey="Gabriel M">M. Gabriel</name></author><author><name sortKey="Emmerich, P" uniqKey="Emmerich P">P. Emmerich</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name></author><author><name sortKey="Held, G" uniqKey="Held G">G. Held</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tetro, J A" uniqKey="Tetro J">J. A. Tetro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thomas, S M" uniqKey="Thomas S">S. M. Thomas</name></author><author><name sortKey="Obermayr, U" uniqKey="Obermayr U">U. Obermayr</name></author><author><name sortKey="Fischer, D" uniqKey="Fischer D">D. Fischer</name></author><author><name sortKey="Kreyling, J" uniqKey="Kreyling J">J. Kreyling</name></author><author><name sortKey="Beierkuhnlein, C" uniqKey="Beierkuhnlein C">C. Beierkuhnlein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tognarelli, J" uniqKey="Tognarelli J">J. Tognarelli</name></author><author><name sortKey="Ulloa, S" uniqKey="Ulloa S">S. Ulloa</name></author><author><name sortKey="Villagra, E" uniqKey="Villagra E">E. Villagra</name></author><author><name sortKey="Lagos, J" uniqKey="Lagos J">J. Lagos</name></author><author><name sortKey="Aguayo, C" uniqKey="Aguayo C">C. Aguayo</name></author><author><name sortKey="Fasce, R" uniqKey="Fasce R">R. Fasce</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ulbert, S" uniqKey="Ulbert S">S. Ulbert</name></author><author><name sortKey="Magnusson, S E" uniqKey="Magnusson S">S. E. Magnusson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Den Berg, B" uniqKey="Van Den Berg B">B. van den Berg</name></author><author><name sortKey="Bunschoten, C" uniqKey="Bunschoten C">C. Bunschoten</name></author><author><name sortKey="Van Doorn, P A" uniqKey="Van Doorn P">P. A. van Doorn</name></author><author><name sortKey="Jacobs, B C" uniqKey="Jacobs B">B. C. Jacobs</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vazquez Calvo, A" uniqKey="Vazquez Calvo A">A. Vazquez-Calvo</name></author><author><name sortKey="Saiz, J C" uniqKey="Saiz J">J. C. Saiz</name></author><author><name sortKey="Mccullough, K C" uniqKey="Mccullough K">K. C. McCullough</name></author><author><name sortKey="Sobrino, F" uniqKey="Sobrino F">F. Sobrino</name></author><author><name sortKey="Martin Acebes, M A" uniqKey="Martin Acebes M">M. A. Martin-Acebes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ventura, C V" uniqKey="Ventura C">C. V. Ventura</name></author><author><name sortKey="Maia, M" uniqKey="Maia M">M. Maia</name></author><author><name sortKey="Bravo Filho, V" uniqKey="Bravo Filho V">V. Bravo-Filho</name></author><author><name sortKey="Gois, A L" uniqKey="Gois A">A. L. Gois</name></author><author><name sortKey="Belfort, R" uniqKey="Belfort R">R. Belfort</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ventura, C V" uniqKey="Ventura C">C. V. Ventura</name></author><author><name sortKey="Maia, M" uniqKey="Maia M">M. Maia</name></author><author><name sortKey="Ventura, B V" uniqKey="Ventura B">B. V. Ventura</name></author><author><name sortKey="Linden, V V" uniqKey="Linden V">V. V. Linden</name></author><author><name sortKey="Araujo, E B" uniqKey="Araujo E">E. B. Araujo</name></author><author><name sortKey="Ramos, R C" uniqKey="Ramos R">R. C. Ramos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vorndam, V" uniqKey="Vorndam V">V. Vorndam</name></author><author><name sortKey="Mathews, J H" uniqKey="Mathews J">J. H. Mathews</name></author><author><name sortKey="Barrett, A D" uniqKey="Barrett A">A. D. Barrett</name></author><author><name sortKey="Roehrig, J T" uniqKey="Roehrig J">J. T. Roehrig</name></author><author><name sortKey="Trent, D W" uniqKey="Trent D">D. W. Trent</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Waehre, T" uniqKey="Waehre T">T. Waehre</name></author><author><name sortKey="Maagard, A" uniqKey="Maagard A">A. Maagard</name></author><author><name sortKey="Tappe, D" uniqKey="Tappe D">D. Tappe</name></author><author><name sortKey="Cadar, D" uniqKey="Cadar D">D. Cadar</name></author><author><name sortKey="Schmidt Chanasit, J" uniqKey="Schmidt Chanasit J">J. Schmidt-Chanasit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Way, J H" uniqKey="Way J">J. H. Way</name></author><author><name sortKey="Bowen, E T" uniqKey="Bowen E">E. T. Bowen</name></author><author><name sortKey="Platt, G S" uniqKey="Platt G">G. S. Platt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weinbren, M P" uniqKey="Weinbren M">M. P. Weinbren</name></author><author><name sortKey="Williams, M C" uniqKey="Williams M">M. C. Williams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weissenbock, H" uniqKey="Weissenbock H">H. Weissenbock</name></author><author><name sortKey="Hubalek, Z" uniqKey="Hubalek Z">Z. Hubalek</name></author><author><name sortKey="Bakonyi, T" uniqKey="Bakonyi T">T. Bakonyi</name></author><author><name sortKey="Nowotny, N" uniqKey="Nowotny N">N. Nowotny</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Welsch, S" uniqKey="Welsch S">S. Welsch</name></author><author><name sortKey="Miller, S" uniqKey="Miller S">S. Miller</name></author><author><name sortKey="Romero Brey, I" uniqKey="Romero Brey I">I. Romero-Brey</name></author><author><name sortKey="Merz, A" uniqKey="Merz A">A. Merz</name></author><author><name sortKey="Bleck, C K" uniqKey="Bleck C">C. K. Bleck</name></author><author><name sortKey="Walther, P" uniqKey="Walther P">P. Walther</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Winkler, G" uniqKey="Winkler G">G. Winkler</name></author><author><name sortKey="Heinz, F X" uniqKey="Heinz F">F. X. Heinz</name></author><author><name sortKey="Kunz, C" uniqKey="Kunz C">C. Kunz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wolfe, N D" uniqKey="Wolfe N">N. D. Wolfe</name></author><author><name sortKey="Kilbourn, A M" uniqKey="Kilbourn A">A. M. Kilbourn</name></author><author><name sortKey="Karesh, W B" uniqKey="Karesh W">W. B. Karesh</name></author><author><name sortKey="Rahman, H A" uniqKey="Rahman H">H. A. Rahman</name></author><author><name sortKey="Bosi, E J" uniqKey="Bosi E">E. J. Bosi</name></author><author><name sortKey="Cropp, B C" uniqKey="Cropp B">B. C. Cropp</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wong, P S" uniqKey="Wong P">P. S. Wong</name></author><author><name sortKey="Li, M Z" uniqKey="Li M">M. Z. Li</name></author><author><name sortKey="Chong, C S" uniqKey="Chong C">C. S. Chong</name></author><author><name sortKey="Ng, L C" uniqKey="Ng L">L. C. Ng</name></author><author><name sortKey="Tan, C H" uniqKey="Tan C">C. H. Tan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zammarchi, L" uniqKey="Zammarchi L">L. Zammarchi</name></author><author><name sortKey="Stella, G" uniqKey="Stella G">G. Stella</name></author><author><name sortKey="Mantella, A" uniqKey="Mantella A">A. Mantella</name></author><author><name sortKey="Bartolozzi, D" uniqKey="Bartolozzi D">D. Bartolozzi</name></author><author><name sortKey="Tappe, D" uniqKey="Tappe D">D. Tappe</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zanluca, C" uniqKey="Zanluca C">C. Zanluca</name></author><author><name sortKey="De Melo, V C" uniqKey="De Melo V">V. C. de Melo</name></author><author><name sortKey="Mosimann, A L" uniqKey="Mosimann A">A. L. Mosimann</name></author><author><name sortKey="Dos Santos, G I" uniqKey="Dos Santos G">G. I. Dos Santos</name></author><author><name sortKey="Dos Santos, C N" uniqKey="Dos Santos C">C. N. Dos Santos</name></author><author><name sortKey="Luz, K" uniqKey="Luz K">K. Luz</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="review-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Front Microbiol</journal-id><journal-id journal-id-type="iso-abbrev">Front Microbiol</journal-id><journal-id journal-id-type="publisher-id">Front. Microbiol.</journal-id><journal-title-group><journal-title>Frontiers in Microbiology</journal-title></journal-title-group><issn pub-type="epub">1664-302X</issn><publisher><publisher-name>Frontiers Media S.A.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27148186</article-id><article-id pub-id-type="pmc">4835484</article-id><article-id pub-id-type="doi">10.3389/fmicb.2016.00496</article-id><article-categories><subj-group subj-group-type="heading"><subject>Microbiology</subject><subj-group><subject>Review</subject></subj-group></subj-group></article-categories><title-group><article-title>Zika Virus: the Latest Newcomer</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Saiz</surname><given-names>Juan-Carlos</given-names></name><xref ref-type="author-notes" rid="fn001"><sup>*</sup></xref><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/154158/overview"></uri></contrib><contrib contrib-type="author"><name><surname>Vázquez-Calvo</surname><given-names>Ángela</given-names></name><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/333280/overview"></uri></contrib><contrib contrib-type="author"><name><surname>Blázquez</surname><given-names>Ana B.</given-names></name><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/144687/overview"></uri></contrib><contrib contrib-type="author"><name><surname>Merino-Ramos</surname><given-names>Teresa</given-names></name><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/154076/overview"></uri></contrib><contrib contrib-type="author"><name><surname>Escribano-Romero</surname><given-names>Estela</given-names></name><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/162811/overview"></uri></contrib><contrib contrib-type="author"><name><surname>Martín-Acebes</surname><given-names>Miguel A.</given-names></name><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/144343/overview"></uri></contrib></contrib-group><aff id="aff1"><institution>Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria</institution><country>Madrid, Spain</country></aff><author-notes><fn fn-type="edited-by"><p>Edited by: <italic>Abraham L. Brass, University of Massachusetts Medical School, USA</italic></p></fn><fn fn-type="edited-by"><p>Reviewed by: <italic>Sarah Rowland-Jones, University of Oxford, UK; Manoj N. Krishnan, Duke-Nus Graduate Medical School, Singapore</italic></p></fn><corresp id="fn001">*Correspondence: <italic>Juan-Carlos Saiz, <email xlink:type="simple">jcsaiz@inia.es</email></italic></corresp><fn fn-type="other" id="fn002"><p>This article was submitted to Virology, a section of the journal Frontiers in Microbiology</p></fn></author-notes><pub-date pub-type="epub"><day>19</day><month>4</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>7</volume><elocation-id>496</elocation-id><history><date date-type="received"><day>23</day><month>2</month><year>2016</year></date><date date-type="accepted"><day>27</day><month>3</month><year>2016</year></date></history><permissions><copyright-statement>Copyright © 2016 Saiz, Vázquez-Calvo, Blázquez, Merino-Ramos, Escribano-Romero and Martín-Acebes.</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Saiz, Vázquez-Calvo, Blázquez, Merino-Ramos, Escribano-Romero and Martín-Acebes</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</license-p></license></permissions><abstract><p>Since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: West Nile, Influenza A, avian flu, dengue, Chikungunya, SARS, MERS, Ebola, and now Zika, the latest newcomer. Zika virus (ZIKV), a flavivirus transmitted by <italic>Aedes</italic> mosquitoes, was identified in 1947 in a sentinel monkey in Uganda, and later on in humans in Nigeria. The virus was mainly confined to the African continent until it was detected in south-east Asia the 1980’s, then in the Micronesia in 2007 and, more recently in the Americas in 2014, where it has displayed an explosive spread, as advised by the World Health Organization, which resulted in the infection of hundreds of thousands of people. ZIKV infection was characterized by causing a mild disease presented with fever, headache, rash, arthralgia, and conjunctivitis, with exceptional reports of an association with Guillain–Barre syndrome (GBS) and microcephaly. However, since the end of 2015, an increase in the number of GBS associated cases and an astonishing number of microcephaly in fetus and new-borns in Brazil have been related to ZIKV infection, raising serious worldwide public health concerns. Clarifying such worrisome relationships is, thus, a current unavoidable goal. Here, we extensively review what is currently known about ZIKV, from molecular biology, transmission routes, ecology, and epidemiology, to clinical manifestations, pathogenesis, diagnosis, prophylaxis, and public health.</p></abstract><kwd-group><kwd>Zika</kwd><kwd>flavivirus</kwd><kwd>outbreak</kwd><kwd>microcephaly</kwd><kwd>zoonosis</kwd></kwd-group><funding-group><award-group><funding-source id="cn001">Ministerio de Economía y Competitividad<named-content content-type="fundref-id">10.13039/501100003329</named-content></funding-source><award-id rid="cn001">E-RTA2013-0013, S2013/ABI-2906, AGL2014-56518-JIN, ZIKA-BIO</award-id></award-group></funding-group><counts><fig-count count="5"></fig-count><table-count count="1"></table-count><equation-count count="0"></equation-count><ref-count count="156"></ref-count><page-count count="19"></page-count><word-count count="0"></word-count></counts></article-meta></front><body><sec><title>The Virus</title><p>Zika virus (ZIKV) is an arbovirus (arthropod-borne virus) classified into the <italic>Flavivirus</italic> genus within the <italic>Flaviviridae</italic> family<sup><xref ref-type="fn" rid="fn01">1</xref></sup>. Flaviviruses are small enveloped single stranded positive RNA viruses that include important human and animal pathogens such as yellow fever virus (YFV), dengue virus (DENV), West Nile virus (WNV), St. Louis encephalitis virus (SLEV), Japanese encephalitis virus (JEV) or tick-borne encephalitis virus (TBEV) (<xref rid="B55" ref-type="bibr">Gould and Solomon, 2008</xref>). Historically, ZIKV was discovered in the course of investigations designed to study the vector responsible for the non-human cycle of yellow fever in Uganda almost 70 years ago. The first isolation was made in April 1947 from the serum of a febrile sentinel rhesus monkey (named Rhesus 766) that was caged in the canopy of Zika Forest, near Lake Victoria (<xref rid="B36" ref-type="bibr">Dick et al., 1952</xref>). The second isolation was made from <italic>Aedes africanus</italic> mosquitoes caught in the same forest in January 1948 (<xref rid="B36" ref-type="bibr">Dick et al., 1952</xref>). Thus, ZIKV received its name from the geographical area where the initial isolations were made. Both isolations were performed by intracerebral inoculation into <italic>Swiss</italic> albino mice of the samples containing the virus (serum from febrile monkey or mosquito homogenates) demonstrating that ZIKV was a filterable transmissible agent (<xref rid="B36" ref-type="bibr">Dick et al., 1952</xref>). These early filtration studies indicated that the size of ZIKV was in the range of about 30–45 nm in diameter (<xref rid="B35" ref-type="bibr">Dick, 1952</xref>). Further transmission electron microscopy analysis of ZIKV infected cells revealed that the virions were spherical particles with an overall diameter of 40–43 nm and a central electron dense core being 28–30 nm in diameter (<xref rid="B12" ref-type="bibr">Bell et al., 1971</xref>; <xref rid="B61" ref-type="bibr">Hamel et al., 2015</xref>). Although there are still no specific studies on the structure of ZIKV, it can be inferred from other flaviviruses (<xref rid="B99" ref-type="bibr">Mukhopadhyay et al., 2005</xref>) that the viral particles should be about 50 nm in diameter, which is compatible with the observations performed for ZIKV. Cryoelectron microscopy reconstructions of flavivirus particles have shown that virions are composed by a central core that contains the capsid or core (C) protein associated with the viral genomic RNA. This nucleocapsid is enclosed into a lipid bilayer derived from the host cell. The membrane (M) and envelope (E) proteins are anchored into the lipid envelope and conform the smooth outer shell of the virion, which is constituted by 180 copies of the M and E proteins arranged as 90 anti-parallel homodimers (<xref rid="B70" ref-type="bibr">Kuhn et al., 2002</xref>; <xref rid="B98" ref-type="bibr">Mukhopadhyay et al., 2003</xref>). Regarding the stability of the virion, it has been described that ZIKV suspensions were most stable at pH of 6.8–7.4 and particles were inactivated at pH of under 6.2 and over 7.8, by potassium permanganate, ether, and temperatures of 58 °C for 30 min, or 60°C for 15 min, but the infectivity was not effectively neutralized with 10% ethanol (<xref rid="B35" ref-type="bibr">Dick, 1952</xref>).</p><sec><title>Genome</title><p>The flavivirus genome is constituted by a single-stranded RNA molecule of positive polarity that, in a similar manner to cellular mRNAs, includes a cap structure at its 5′ end (<xref rid="B38" ref-type="bibr">Dong et al., 2014</xref>). Proper methylation of this structure is important not only for efficient translation of viral genome, but also for evasion of immune response (<xref rid="B26" ref-type="bibr">Daffis et al., 2010</xref>). The sequence of the prototype strain of ZIKV MR766, which corresponds to a passaged virus derived from the initial ZIKV isolated by intracerebral inoculation of the serum of the febrile monkey (Rhesus 766) into mice in 1947 (<xref rid="B35" ref-type="bibr">Dick, 1952</xref>; <xref rid="B36" ref-type="bibr">Dick et al., 1952</xref>), revealed that the ZIKV genome was 10794 nucleotides in length (<xref rid="B71" ref-type="bibr">Kuno and Chang, 2007</xref>). The genome contains a single open reading frame (ORF) that encodes a polyprotein of about 3400 amino acids (<bold>Figure <xref ref-type="fig" rid="F1">1</xref></bold>) that is expected to be cleaved into the mature viral proteins (see next section for polyprotein processing). The single ORF is flanked by two untraslated regions (UTR) located at the 5′ and 3′ ends of the genome, which in the prototype ZIKV MR766 are of 106 and 428 nucleotides in length, respectively (<xref rid="B71" ref-type="bibr">Kuno and Chang, 2007</xref>). Remarkably, and in contrast to cellular mRNAs, ZIKV genome lacks a 3′ poly(A) tract and ends with CU<sub>OH</sub> in a similar manner to the other flaviviruses. Subsequent studies have confirmed that this basic organization is shared among other isolates of ZIKV, although differences in length and nucleotide sequence have been documented among different isolates, even among ZIKV MR766 isolates with different passage history (<xref rid="B76" ref-type="bibr">Lanciotti et al., 2008</xref>; <xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>; <xref rid="B10" ref-type="bibr">Baronti et al., 2014</xref>; <xref rid="B15" ref-type="bibr">Berthet et al., 2014</xref>). The cyclization of flavivirus genome between 5′ and 3′ terminal regions, which is important for the functionality of the genome, is mediated by the interaction of complementary sequences located with genome regions termed conserved sequences (CSs). These CS (CS1 to CS3) are also present in the ZIKV genome, suggesting that has the potential for cyclization. Nevertheless, it has to be remarked that the organization of the CS in the 3′end of ZIKV is different from that of other mosquito-borne flaviviruses (<xref rid="B71" ref-type="bibr">Kuno and Chang, 2007</xref>).</p><fig id="F1" position="float"><label>FIGURE 1</label><caption><p><bold>Schematic view of Zika virus (ZIKV) genome organization.</bold> The open reading frame (ORF) (boxes) that encodes structural and non-structural proteins is flanked by two untranslated regions (UTR). The proportion of each region was calculated from the ZIKV MR766 sequence available at GenBank (NC_012532.1). Scale bar: 1 kb.</p></caption><graphic xlink:href="fmicb-07-00496-g001"></graphic></fig></sec><sec><title>Viral Proteins</title><p>The viral polyprotein encoded by the single ORF in ZIKV (<bold>Figure <xref ref-type="fig" rid="F1">1</xref></bold>), as in other related flaviviruses, is supposed to be cleaved by cellular and viral proteases into three structural proteins: the capsid (C), premembrane/membrane (prM/M), and envelope (E), and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). The predicted cleavage sites of ZIKV basically follow the patterns established for other mosquito-borne viruses, and, likewise, cysteine residues within the polyprotein are well conserved relative to other mosquito-borne flavivirus (<xref rid="B71" ref-type="bibr">Kuno and Chang, 2007</xref>). Different proteases participate in the processing of the viral polyprotein: the host cellular signalase which cleaves M/E, E/NS1, and the C-terminal hydrophobic region of NS4A (termed 2K peptide)/NS4B. The viral serine protease (NS3) is expected to cleave the junctions between the virion capsid protein (C<sub>v</sub>) and the C-terminal hydrophobic domain of capsid protein (C<sub>i</sub>) [C<sub>v</sub>/C<sub>i</sub>], NS2A/NS2B, NS2B/NS3, NS3/NS4A, NS4A/2K peptide, and NS4B/NS5. The NS1/NS2A is believed to be cleaved by an unknown cellular signalase (<xref rid="B71" ref-type="bibr">Kuno and Chang, 2007</xref>). Of key importance is the proteolytic cleavage of prM to give the pr peptide and M protein, which is produced by furin-like protease located in the <italic>trans</italic>-Golgi network during the egress of the particles and promote the maturation of the virions (<xref rid="B99" ref-type="bibr">Mukhopadhyay et al., 2005</xref>).</p><p>The analysis of the polyprotein sequence predicts the presence of potential N-glycosylation sites in the ZIKV proteins prM, E, and NS1 (<xref rid="B71" ref-type="bibr">Kuno and Chang, 2007</xref>; <xref rid="B10" ref-type="bibr">Baronti et al., 2014</xref>; <xref rid="B15" ref-type="bibr">Berthet et al., 2014</xref>). However, the functional significance of the <italic>N</italic>-glycosylations is not clear in related flaviviruses, since deglycosylated flaviviruses can maintain the same antigenicity, suggesting that carbohydrate does not play a major role in the antigenic properties of the virus (<xref rid="B152" ref-type="bibr">Winkler et al., 1987</xref>), and that glycosylation does not alter epitope recognition (<xref rid="B146" ref-type="bibr">Vorndam et al., 1993</xref>). On the other hand, glycosylation could be important for replication and maturation (<xref rid="B79" ref-type="bibr">Li et al., 2006</xref>). In the case of ZIKV, there are differences among strains due to a 12 nucleotides deletion on the glycosylation motif located at position 154 in the E protein (E-154), which is present in many flaviviruses (<xref rid="B76" ref-type="bibr">Lanciotti et al., 2008</xref>; <xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>; <xref rid="B10" ref-type="bibr">Baronti et al., 2014</xref>). Remarkably, there are differences on this site even between ZIKV isolates with different passage history, such as those of the prototypic strain ZIKV MR766, indicating that passage history influences glycosylation sites (<xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>). However, the loss of glycosylation on the E-154 residue is not unique to ZIKV and has also been observed in other flaviviruses (<xref rid="B2" ref-type="bibr">Adams et al., 1995</xref>; <xref rid="B14" ref-type="bibr">Berthet et al., 1997</xref>). Although the functional role of glycosylation in the E protein is not clear, the presence of this glycosylation in other flaviviruses has been associated with the ability to cause significant human outbreaks (<xref rid="B129" ref-type="bibr">Shirato et al., 2004</xref>). Moreover, it has been suggested that the fact that ZIKV strains isolated during the recent human outbreak in Oceania contain this N-linked glycosylation signal, whereas the majority of other strains does not, could indicate that the N-linked glycosylation of the E protein plays a role on the pathogenicity of ZIKV (<xref rid="B10" ref-type="bibr">Baronti et al., 2014</xref>; <xref rid="B15" ref-type="bibr">Berthet et al., 2014</xref>). Nevertheless, functional studies are required to provide an experimental confirmation of this hypothesis.</p><p>Regarding the functions of the flaviviral proteins, the three structural proteins participate in the assembly of the virions. As commented above, the C protein associates with the genomic RNA to conform the core of the virions, and the E protein should mediate the binding to the cellular receptor of the virus and promotes the fusion of the virions with the endosomal membranes of the target cell during viral entry (<xref rid="B99" ref-type="bibr">Mukhopadhyay et al., 2005</xref>; <xref rid="B121" ref-type="bibr">Roby et al., 2015</xref>). Relative to prM function, this protein assists the folding of E protein as a sort of chaperone and prevents premature fusion of the particles prior to be released from the infected cell, and the cleavage of prM into M protein also promotes the maturation of the viral particles (<xref rid="B99" ref-type="bibr">Mukhopadhyay et al., 2005</xref>; <xref rid="B121" ref-type="bibr">Roby et al., 2015</xref>).</p><p>To our knowledge there are currently no specific studies addressing the function of non-structural proteins of ZIKV, but it is expected that some functions could be inferred from related flaviviruses (<xref rid="B88" ref-type="bibr">Martin-Acebes and Saiz, 2012</xref>; <xref rid="B1" ref-type="bibr">Acosta et al., 2014</xref>), such as the induction of membrane rearrangements associated with flavivirus replication (NS4A), and the immunomodulation (NS1, NS2A) or regulation of RNA replication and viral assembly (NS2A). Furthermore, NS2B acts as a cofactor for the viral trypsin-like serine protease NS3, which can also act as a helicase. NS5 is the viral RNA dependent RNA polymerase that is in charge of genome replication, and that also displays a methyltransferase domain necessary for capping the 5′ end of the viral genomic RNA. Since flavivirus non-structural proteins constitute major targets for antiviral research (<xref rid="B105" ref-type="bibr">Noble and Shi, 2012</xref>; <xref rid="B82" ref-type="bibr">Luo et al., 2015</xref>), deciphering the specific role of the non-structural proteins in ZIKV infection could greatly contribute to the development of antiviral strategies against this pathogen.</p></sec><sec><title>Host Cell-Virus Interactions</title><p>Zika virus can infect a broad range of cells from different tissues and species. For instance, experimental infection by blood meal has revealed that ZIKV replicates in the midgut and salivary glands of diverse <italic>Aedes</italic> mosquitoes (<xref rid="B80" ref-type="bibr">Li et al., 2012</xref>; <xref rid="B154" ref-type="bibr">Wong et al., 2013</xref>), and also <italic>in vitro</italic> in cultured mosquito cells C6/36 (<xref rid="B61" ref-type="bibr">Hamel et al., 2015</xref>). ZIKV also replicates in a wide variety of mammalian cell types. Experimental infection in mice has revealed that the virus replicates mainly in brain cells, including neurons, and astroglial cells (<xref rid="B149" ref-type="bibr">Weinbren and Williams, 1958</xref>; <xref rid="B12" ref-type="bibr">Bell et al., 1971</xref>), and, <italic>in vitro</italic>, it can replicate in cultured monkey cell lines such as LLC- MK2, or Vero, inducing cytopathic effect (<xref rid="B148" ref-type="bibr">Way et al., 1976</xref>). Furthermore, the titer of ZIKV in cultured cells seems to well correlate with the infectivity of the virus <italic>in vivo</italic> (<xref rid="B148" ref-type="bibr">Way et al., 1976</xref>). In addition, it has also been recently reported that ZIKV can replicate in human skin cells and also in immature dendritic cells (<xref rid="B61" ref-type="bibr">Hamel et al., 2015</xref>). This ability of the virus to replicate in cells from different sources could be related to its transmission cycle, which includes replication in mosquito (vector) and mammalian cells (host).</p><p>It has been described that ZIKV enters the cell using adhesion factors such as DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin) and diverse members of the phosphatidylserine receptor family (<xref rid="B61" ref-type="bibr">Hamel et al., 2015</xref>). Once the attached viral particles are internalized into the cell (<bold>Figure <xref ref-type="fig" rid="F2">2</xref></bold>), the viral genome should be released inside the cytoplasm to start translation and replication. The mechanism of penetration of the flavivirus genome into the cytoplasm is initiated by the fusion of the viral envelope with the membranes of the cellular endosomes from the host cell, a process triggered by acidic pH inside cellular endosomes (<xref rid="B135" ref-type="bibr">Stiasny et al., 2011</xref>; <xref rid="B143" ref-type="bibr">Vazquez-Calvo et al., 2012</xref>). This mechanism of penetration is consistent with, as mentioned before, an early observation showing that ZIKV particles were sensible to acidic pH, and were inactivated by treatment with acidic pH lower than 6.2 (<xref rid="B35" ref-type="bibr">Dick, 1952</xref>). Along this line, the sensitivity of ZIKV particles to acidic pH is consistent with the observation performed with other flaviviruses indicating that, in the absence of target membranes, the exposure of flavivirus virions to acidic pH induces rearrangements of the E glycoprotein, which result in a loss of infectivity (<xref rid="B54" ref-type="bibr">Gollins and Porterfield, 1986</xref>). The viral RNA acts as mRNA inside the cytoplasm of the infected cell, and negative-strand viral RNA is synthesized and directs positive-strand RNA synthesis in association with a virus-induced network of membranes derived from the endoplasmic reticulum, ER (<bold>Figure <xref ref-type="fig" rid="F2">2</xref></bold>). Electron microscopy studies of virus infected cells showed that ZIKV virions are found in short chains within tubular elements of the ER, which appeared to be in continuity with distended cisternae (<xref rid="B12" ref-type="bibr">Bell et al., 1971</xref>). These images were similar to membrane rearrangements observed in other flavivirus infected cells (<xref rid="B151" ref-type="bibr">Welsch et al., 2009</xref>; <xref rid="B87" ref-type="bibr">Martin-Acebes et al., 2011</xref>; <xref rid="B93" ref-type="bibr">Miorin et al., 2013</xref>). Although flavivirus replication is thought to occur in the cellular cytoplasm, it should be noted that one study reported that ZIKV antigens could be found in infected cell nuclei (<xref rid="B20" ref-type="bibr">Buckley and Gould, 1988</xref>).</p><fig id="F2" position="float"><label>FIGURE 2</label><caption><p><bold>Schematic view of the ZIKV cellular lifecycle.</bold> See text for details.</p></caption><graphic xlink:href="fmicb-07-00496-g002"></graphic></fig><p><italic>De novo</italic> synthesized positive strand-RNA has to be packaged in progeny virions that bud into the ER to form enveloped immature virions (<bold>Figure <xref ref-type="fig" rid="F2">2</xref></bold>). These virions traffic through the Golgi complex and, then, the prM is cleaved in the <italic>trans</italic>-Golgi network for particle maturation prior to release from the infected cell (<xref rid="B99" ref-type="bibr">Mukhopadhyay et al., 2005</xref>; <xref rid="B121" ref-type="bibr">Roby et al., 2015</xref>). Remarkably, the observations made for other flaviviruses have revealed that not all the copies of the prM protein in the secreted virions are cleaved, and, thus, that a proportion of them remains unprocessed in the secreted virions (<xref rid="B113" ref-type="bibr">Plevka et al., 2011</xref>). Even more, the amount of prM within the flavivirus virions varies with the cell line used for the production of the virus and the infecting flavivirus, and could be of key importance for the antigenicity of the particles (<xref rid="B112" ref-type="bibr">Pierson and Diamond, 2012</xref>; <xref rid="B81" ref-type="bibr">Lok, 2016</xref>).</p><p>Knowledge regarding the cellular response to ZIKV infection is still scarce. However, it has been experimentally probed that replication of ZIKV provokes an innate antiviral response, inducing the transcription of TLR3, RIG-I, and MDA5, as well as that of several interferon stimulated genes, such as OAS2, ISG15, and MX1, characterized by a strongly enhanced beta interferon gene expression (<xref rid="B61" ref-type="bibr">Hamel et al., 2015</xref>). In addition, ZIKV infection is sensitive to interferon (IFN) signaling, as pretreatment of primary skin fibroblasts with IFN-alpha, beta, and gamma reduces ZIKV infection (<xref rid="B61" ref-type="bibr">Hamel et al., 2015</xref>). ZIKV infection also upregulates the autophagic pathway in infected skin fibroblasts (<xref rid="B61" ref-type="bibr">Hamel et al., 2015</xref>), which is consistent with the observations for other related flaviviruses (<xref rid="B18" ref-type="bibr">Blazquez et al., 2014</xref>). Moreover, the autophagic marker LC3 colocalizes with viral proteins within ZIKV-infected cells, and the infection can be reduced by treatment with the autophagy inhibitor 3-methyladenine, whereas upregulation of autophagy using Torin 1 increases ZIKV replication (<xref rid="B61" ref-type="bibr">Hamel et al., 2015</xref>).</p></sec></sec><sec><title>Molecular Classification</title><p>Zika virus is genetically and antigenically related to Spondweni virus. Both viruses form a unique clade (clade X) within the mosquito-borne flavivirus cluster (<xref rid="B72" ref-type="bibr">Kuno et al., 1998</xref>) (<bold>Figure <xref ref-type="fig" rid="F3">3</xref></bold>). Phylogenetic analyses reveal the existence of two major lineages: one includes the African strains, and the other the Asian and American strains (<xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>; <xref rid="B4" ref-type="bibr">Alera et al., 2015</xref>) (<bold>Figure <xref ref-type="fig" rid="F4">4</xref></bold>). The African lineage is further divided into two groups, the East African cluster, containing the genetic variants of the prototypic MR766 strain isolated in Uganda in 1947, and a second group including West African strains (<xref rid="B109" ref-type="bibr">Olson et al., 1981</xref>).</p><fig id="F3" position="float"><label>FIGURE 3</label><caption><p><bold>Representative phylogram showing the relationships between strains of the genus Flavivirus.</bold> Accession numbers are displayed in the tree. The arrow indicates ZIKV. The scale indicates 0.2 substitutions/site. The tree was based on complete NS5 nucleotide sequence, built from a multiple alignment using Clustal omega and Phylogeny.fr (<xref rid="B32" ref-type="bibr">Dereeper et al., 2008</xref>).</p></caption><graphic xlink:href="fmicb-07-00496-g003"></graphic></fig><fig id="F4" position="float"><label>FIGURE 4</label><caption><p><bold>Representative phylogram showing the relationships between ZIKV strains.</bold> The accession number, country of isolation and year is displayed in the tree. The scale indicates 0.03 substitutions/site. The tree was based on complete NS5 nucleotide sequence, built from a multiple alignment using Clustal omega and Phylogeny.fr.</p></caption><graphic xlink:href="fmicb-07-00496-g004"></graphic></fig><p>Phylogenetic studies (<xref rid="B47" ref-type="bibr">Faye et al., 2014</xref>) have established the date of the emergence of ZIKV in east Africa around 1920 (confidence range of 1892–1947). The same study dated the transmission of eastern African ZIKV to Asia around 1945 (confidence range 1920–1960), where the virus was first detected in the late 1960s in Malaysia (<xref rid="B85" ref-type="bibr">Marchette et al., 1969</xref>), and subsequently across south-east Asia. These data indicated a widespread occurrence of ZIKV from Africa to Southeast Asia, west and north of the Wallace line (<xref rid="B76" ref-type="bibr">Lanciotti et al., 2008</xref>).</p><p>Phylogenetic studies have confirmed that Pacific Island ZIKV strains are related to the Asian lineages (<xref rid="B51" ref-type="bibr">Gatherer and Kohl, 2016</xref>). Due to the great geographical distances involved, it seems likely that the virus was introduced to the island either by a viremic person, an enzootic host species, or an infected mosquito transported to the island (<xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>). On the other hand, recent transmission to the Americas appears to have originated in the Pacific Islands (<xref rid="B23" ref-type="bibr">Campos et al., 2015</xref>; <xref rid="B156" ref-type="bibr">Zanluca et al., 2015</xref>). Phylogenetic analysis of the sequences placed the Brazilian and other American strains in a clade with sequences from the Asian lineage, showing a 99% identity with a sequence from a ZIKV isolate from French Polynesia (<xref rid="B10" ref-type="bibr">Baronti et al., 2014</xref>; <xref rid="B23" ref-type="bibr">Campos et al., 2015</xref>) (<bold>Figure <xref ref-type="fig" rid="F4">4</xref></bold>). It has been postulated that two events may have led to the introduction of ZIKV in Brazil, the 2014 FIFA World Cup tournament and an international canoe racing event (<xref rid="B100" ref-type="bibr">Musso, 2015</xref>), but since Pacific nations were only represented among the canoe racers, the latter seems to be a likeliest introduction route.</p><p>As exemplified in <bold>Figure <xref ref-type="fig" rid="F4">4</xref></bold>, ZIKV strains collected in the same geographical region during several years show minimal changes on their sequences, as is the case of strains collected from mosquitoes in a 3 years interval in Central African Republic (<xref rid="B15" ref-type="bibr">Berthet et al., 2014</xref>). In this regard, it has been described that infection and transmission modes of ZIKV allow the accumulation of synonymous mutations and negatively selected certain sites (<xref rid="B47" ref-type="bibr">Faye et al., 2014</xref>). The arbovirus life cycle imposes several barriers to non-synonymous mutations in some important genes as a consequence of the intrinsic constraints associated with dual replication in mammalian and invertebrate hosts, thus driving to a more slowly fixation of mutations of these viruses when compared with RNA viruses transmitted by other routes. In fact, arboviruses, which are able to successfully adapt to diverse cell types, are characterized by a high rate of deleterious mutations (<xref rid="B65" ref-type="bibr">Holmes, 2003</xref>).</p><p>Human-to-human transmission of the Asian ZIKV strains along the Pacific Islands and South America has been associated with significant NS1 codon usage adaptation to human housekeeping genes, which could facilitate viral replication and increase viral titers (<xref rid="B50" ref-type="bibr">Freire et al., 2015</xref>). Furthermore, this report predicted the presence of several epitopes in the NS1 protein that are shared between ZIKV and DENV, pointing to a significant dependence of the recent human ZIKV spread on NS1 translational selection.</p><p>As mentioned above, it is noteworthy to note that several of the ZIKV strains exhibited a 4 amino acid deletion corresponding to the envelope protein 154 glycosylation motif found in many flaviviruses (<xref rid="B15" ref-type="bibr">Berthet et al., 2014</xref>).</p></sec><sec><title>Transmission Cycle</title><sec><title>Mosquitoes</title><p>The arthropod vectors of the ZIKV natural transmission cycle are mosquitoes of the genus <italic>Aedes</italic> (<xref rid="B33" ref-type="bibr">Diagne et al., 2015</xref>). As mentioned early, the virus was first isolated from <italic>A. africanus</italic> (<xref rid="B36" ref-type="bibr">Dick et al., 1952</xref>) and, since then, ZIKV has been isolated from <italic>A. aegypti</italic> (<xref rid="B85" ref-type="bibr">Marchette et al., 1969</xref>), the main vector of the virus, and also from <italic>A. albopictus</italic> (<xref rid="B57" ref-type="bibr">Grard et al., 2014</xref>), confirming that both species are competent vectors.</p><p><italic>Aedes aegypti</italic> is currently distributed in Asia and Oceania, the Americas, and in a few regions of Africa and Europe (Madeira and the north-eastern Black Sea coast)<sup><xref ref-type="fn" rid="fn02">2</xref></sup>; however, it has been recently predicted that this species might soon colonize some southern European regions, as well as temperate North America and Australia (<xref rid="B69" ref-type="bibr">Kraemer et al., 2015</xref>). The first evidence of the role of <italic>A. aegypti</italic> in the urban transmission cycle of ZIKV was suggested after its isolation from a pool of mosquitoes collected in 1966 in Malaysia, in what it was the first isolation from a mosquito other than <italic>A. africanus</italic> (<xref rid="B85" ref-type="bibr">Marchette et al., 1969</xref>).</p><p><italic>Aedes albopictus</italic>, the so-called Asian tiger mosquito, is also widely distributed. This species is currently circulating in Asia, North, Central and South America (<xref rid="B69" ref-type="bibr">Kraemer et al., 2015</xref>), northern Australia, and in some areas of Africa and southern Europe, where it has spread in the past two decades to France, Germany, Italy, and Spain (<xref rid="B110" ref-type="bibr">Paupy et al., 2009</xref>; <xref rid="B41" ref-type="bibr">Dyer, 2016</xref>). Contrary to <italic>A. aegypti, A. albopictus</italic> can hibernate and survive in cool temperature regions (<xref rid="B139" ref-type="bibr">Thomas et al., 2012</xref>). This species can also efficiently transmit the virus, as demonstrated during the outbreak that took place in Gabon in 2007, where, among all species tested (including <italic>A. aegypti</italic>), it was the only one in which the virus was detected, thus confirming that <italic>A. albopictus</italic> may also play an important role in ZIKV transmission (<xref rid="B57" ref-type="bibr">Grard et al., 2014</xref>).</p><p>The ability of ZIKV to be efficiently transmitted by both mosquito species (<italic>A. aegypti</italic> and <italic>A. albopictus</italic>) that feed on humans further complicates their control and, thus, that of ZIKV. Both species grow very close to human populations, but while <italic>A. aegypti</italic> feed almost exclusively on humans in daylight hours and typically rest indoors (<xref rid="B127" ref-type="bibr">Scott and Takken, 2012</xref>), <italic>A. albopictus</italic> is usually exophagic and bites humans and also domestic and livestock animals (<xref rid="B110" ref-type="bibr">Paupy et al., 2009</xref>), although under some circumstances it preferentially feed on humans, hence, confirming that it can also have an anthropophilic behavior similar to <italic>A. aegypti</italic> (<xref rid="B115" ref-type="bibr">Ponlawat and Harrington, 2005</xref>; <xref rid="B30" ref-type="bibr">Delatte et al., 2010</xref>). Therefore, methods of control for a species may not be accurate to control the other one. Furthermore, when the populations of <italic>A. aegypti</italic> is reduced, the opportunistic invasive <italic>A. albopictus</italic> may rapidly move into the area (<xref rid="B64" ref-type="bibr">Higgs, 2016</xref>).</p><p><italic>Aedes aegypti</italic> and, to a lesser extent, <italic>A. albopticus</italic> are clearly involved in ZIKV transmission and spread, but other <italic>Aedes</italic> spp., such as <italic>A. polynesienis</italic>, are suspected to have also contributed to it, as it was the case during the 2013–2014 outbreak in the French Polynesia (<xref rid="B24" ref-type="bibr">Cao-Lormeau et al., 2014</xref>). In fact, ZIKV has been also isolated from, at least, other 15 <italic>Aedes</italic> species in different regions of the world (<bold>Table <xref ref-type="table" rid="T1">1</xref></bold>). Even more, in the Kédougou region of Senegal, ZIKV was also amplified from pools of three mosquito species other than <italic>Aedes: Anopheles coustani, Culex perfuscus</italic>, and <italic>Mansonia uniformis</italic> (<xref rid="B34" ref-type="bibr">Diallo et al., 2014</xref>).</p><table-wrap id="T1" position="float"><label>Table 1</label><caption><p>Zika vector mosquito species.</p></caption><table frame="hsides" rules="groups" cellspacing="5" cellpadding="5"><thead><tr><th valign="top" align="left" rowspan="1" colspan="1">Species</th><th valign="top" align="left" rowspan="1" colspan="1">Arguments in favor of vector status</th><th valign="top" align="left" rowspan="1" colspan="1">Place and year of first report (Reference)</th></tr></thead><tbody><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes africanus</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Zika Forest (Uganda), 1948 (<xref rid="B36" ref-type="bibr">Dick et al., 1952</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes aegypti</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Malaysia, 1966 (<xref rid="B85" ref-type="bibr">Marchette et al., 1969</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes luteocephalus</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Saboya Forest (Senegal), 1968 (<xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>; <xref rid="B34" ref-type="bibr">Diallo et al., 2014</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes vittatus</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Senegal 1968 (<xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes dalzieli</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Senegal 1968 (<xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes metallicus</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Senegal 1968 (<xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Mansonia uniformis</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Senegal 1968 (<xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes fowleri</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Senegal 1968 (<xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes minutus</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Senegal 1968 (<xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes neoafricanus</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Senegal 1968 (<xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes tarsalis</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Senegal 1968 (<xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes apicoargenteus</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Central African Republic, 1969 (<xref rid="B33" ref-type="bibr">Diagne et al., 2015</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes furcifer-taylori</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Bandia (Senegal), 1969 (<xref rid="B34" ref-type="bibr">Diallo et al., 2014</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Annopheles gambiae s.l.</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Bandia (Senegal), 1969 (<xref rid="B34" ref-type="bibr">Diallo et al., 2014</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes opok</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Central African Republic, 1976–1980 (<xref rid="B15" ref-type="bibr">Berthet et al., 2014</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes taylori</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Isolation</td><td valign="top" align="left" rowspan="1" colspan="1">Kédougou region (Senegal), 1988–1991 (<xref rid="B97" ref-type="bibr">Monlun et al., 1993</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes albopictus</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Epidemiology</td><td valign="top" align="left" rowspan="1" colspan="1">Gabon, 2007 (<xref rid="B57" ref-type="bibr">Grard et al., 2014</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes hirsutus</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Epidemiology</td><td valign="top" align="left" rowspan="1" colspan="1">Kédougou region (Senegal), 2011, (<xref rid="B34" ref-type="bibr">Diallo et al., 2014</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Aedes unilinaetus</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Epidemiology</td><td valign="top" align="left" rowspan="1" colspan="1">Kédougou region (Senegal), 2011, (<xref rid="B34" ref-type="bibr">Diallo et al., 2014</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Culex perfuscus</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Epidemiology</td><td valign="top" align="left" rowspan="1" colspan="1">Kédougou region (Senegal), 2011, (<xref rid="B34" ref-type="bibr">Diallo et al., 2014</xref>)</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><italic>Anopheles coustani</italic></td><td valign="top" align="left" rowspan="1" colspan="1">Epidemiology</td><td valign="top" align="left" rowspan="1" colspan="1">Kédougou region (Senegal), 2011, (<xref rid="B34" ref-type="bibr">Diallo et al., 2014</xref>)</td></tr></tbody></table></table-wrap><p>As in other arboviral infections, local overwintering could be an important aspect for maintenance and spread of ZIKV. Detection of the virus in a pool of <italic>A. furcifer</italic> males in 2011 in Senegal, even though no infected <italic>A. furcifer</italic> females were collected, strongly suggested that ZIKV is vertically transmitted, at least in this species, and that this transmission route may be an important mechanism of local maintenance (<xref rid="B34" ref-type="bibr">Diallo et al., 2014</xref>).</p><p>Finally, although most of these data were obtained by analyses of ZIKV naturally infected mosquitoes, their current vector competence still has to be clearly established. In this sense, different <italic>Aedes</italic> species (<italic>A. aegypti, A. unilineatus, A. vittatus</italic>, and <italic>A. luteocephalus</italic>) were recently tested in their susceptibility to ZIKV oral infection, and, although all of them were susceptible, viral genome could be amplified from saliva only in the case of <italic>A. vittatus</italic> and <italic>A. luteocephalus</italic> mosquitoes (<xref rid="B33" ref-type="bibr">Diagne et al., 2015</xref>).</p></sec><sec><title>Humans and Non-human Primates</title><p>During outbreaks, humans are the primary host for ZIKV (<xref rid="B134" ref-type="bibr">Staples et al., 2016</xref>), and both urban (<xref rid="B57" ref-type="bibr">Grard et al., 2014</xref>) and sylvatic (<xref rid="B15" ref-type="bibr">Berthet et al., 2014</xref>) viral transmission have been demonstrated. First ZIKV isolation from humans was reported in 1954 in Nigeria (<xref rid="B83" ref-type="bibr">Macnamara, 1954</xref>), although antibodies to the virus had been previously found during early surveys on human sera in different regions of Africa (<xref rid="B35" ref-type="bibr">Dick, 1952</xref>; <xref rid="B131" ref-type="bibr">Smithburn, 1952</xref>). Recently, and albeit an estimated 80% of ZIKV infected people are asymptomatic<sup><xref ref-type="fn" rid="fn03">3</xref></sup>, during the ongoing outbreak in Brazil, ZIKV RNA has been identified in brain, placenta, and amniotic fluid specimens, and its presence has been associated to microcephaly in infants and miscarries during pregnancy (<xref rid="B89" ref-type="bibr">Martines et al., 2016</xref>; <xref rid="B94" ref-type="bibr">Mlakar et al., 2016</xref>).</p><p>One important aspect that still has to be clarified is whether ZIKV infection in humans drives to viral titers enough to initiate a new cycle when an infected person is bitten by a naïve mosquito. Early studies attempting to infect <italic>A. aegypti</italic> from a ZIKV-infected human volunteer to further transmit the agent to newborns mice were unsuccessful (<xref rid="B11" ref-type="bibr">Bearcroft, 1956</xref>), probably because viremia was too low, even though the volunteer was bitten during the acute phase of the disease (4–6 days after infection). Later on, a recent attempt to isolate circulating virus from infected patients of the 2007 Gabonese outbreak on mammalian (Vero) and insect (C6/36) cell lines was again unsuccessful, presumably because of low viral titers (despite two patients presenting only 1 and 4 days after symptom onset), although the inappropriate initial storage conditions could have also contributed to it (<xref rid="B57" ref-type="bibr">Grard et al., 2014</xref>). In this line, the estimated number of genome copies circulating in ZIKV-infected patients during the 2007 outbreak on the Pacific Island of Yap was reported to be 0.9 × 10<sup>3</sup>–7.2 × 10<sup>5</sup> cDNA copies/ml (<xref rid="B76" ref-type="bibr">Lanciotti et al., 2008</xref>). These relatively low levels of viremia among ZIKV-infected patients are very far from those reported for other arboviruses, as Chikungunya (CHIK) and DENV-2, where an estimated 10<sup>7</sup> and 10<sup>8</sup> cDNA copies/ml were reported, respectively (<xref rid="B25" ref-type="bibr">Caron et al., 2012</xref>), but they are in the range of other dead-end flavivirus infection in humans, such as that of WNV, where viral loads from 50 to 6.9 × 10<sup>5</sup> copies/ml are observed (<xref rid="B117" ref-type="bibr">Pupella et al., 2013</xref>). Therefore, further experiments are needed to clarify this issue.</p><p>In the case of non-human primates, it is known that epizootics occur in them (<xref rid="B91" ref-type="bibr">McCrae and Kirya, 1982</xref>), but it is unclear whether they are an obligatory reservoir in the transmission to humans. In Africa, ZIKV natural transmission cycle involves primarily <italic>Cercopithecus aethiops</italic> and <italic>Erythrocebus patas</italic> monkeys (<xref rid="B45" ref-type="bibr">Faye et al., 2013</xref>). In Asia (Borneo), antibodies against ZIKV have been detected among semi-captive and wild orangutans (<xref rid="B153" ref-type="bibr">Wolfe et al., 2001</xref>). However, this study reported a higher prevalence of anti-ZIKV antibodies in humans than in orangutans, suggesting a possible incidental infection of these animals through contact with mosquitoes infected by viremic people, or from recently established sylvatic cycles. Nonetheless, it is also possible that sylvatic ZIKV transmitting mosquitoes in Borneo have a more narrow distribution, or an ecology that does not lead to frequent exposure by orangutans.</p><p>An acute symptomatic ZIKV infection case after a monkey bite has been recently described (<xref rid="B78" ref-type="bibr">Leung et al., 2015</xref>). Even though transmission throughout mosquito bite could not be completely discharged, the presence of ZIKV in the human pharynx and the previous identification of ZIKV RNA in saliva from asymptomatic infected patients (<xref rid="B16" ref-type="bibr">Besnard et al., 2014</xref>) could be consistent with a potential transmission from primates’ bite (<xref rid="B78" ref-type="bibr">Leung et al., 2015</xref>).</p></sec><sec><title>Other Vertebrates</title><p>Information regarding the possible susceptibility of animals other than human and non-human primates is limited. Antibodies directed against ZIKV have been found in several vertebrates species, such rodents, birds, reptiles, goats, sheep, and cattle in Kenya (<xref rid="B68" ref-type="bibr">Johnson et al., 1977</xref>), and in Pakistan, where some species of rodents were suggested as possible reservoirs of ZIKV (<xref rid="B27" ref-type="bibr">Darwish et al., 1983</xref>). In addition, the rapid periodicity of amplification observed in Senegal along the 2011 outbreak could support that, besides primates, other vertebrates may also play a role in ZIKV circulation (<xref rid="B34" ref-type="bibr">Diallo et al., 2014</xref>).</p></sec><sec><title>Non-vector</title><p>Sporadic reports of direct human-to-human transmission have been reported to occur perinatally, sexually, and through breastfeeding and blood transfusion. Likewise, occupational transmission in the laboratory setting has also been described (<xref rid="B48" ref-type="bibr">Filipe et al., 1973</xref>).</p><p>Perinatal transmission from two mothers to their new-borns during the French Polynesia outbreak has been documented, although contamination during delivery was not completely discarded (<xref rid="B16" ref-type="bibr">Besnard et al., 2014</xref>). Sera from the mothers were positive for ZIKV by reverse transcriptase polymerase chain reaction (RT-PCR) within 2 days post-delivery and those of their newborns within 4 days after birth. In addition, high ZIKV RNA load was detected in breast milk samples from both mothers, albeit the virus could not be multiplied in susceptible cell cultures. Therefore, ZIKV transmission by breastfeeding must be considered and further clarify. Similarly, a first case of perinatal transmission of ZIKV was suspected to have occurred during the same outbreak from a mother that present a ZIKV infection-like syndrome 2 weeks before delivery, with the newborn showing a maculopapular rash at birth; however, virological investigations were not performed in this case (<xref rid="B16" ref-type="bibr">Besnard et al., 2014</xref>).</p><p>Besides these sporadic cases of non-vector transmission, the most surprising phenomenon on ZIKV infection is, without any doubt, the unexpected number of infants supposedly born with microcephaly (see clinical manifestations and pathogenesis section below) in Brazil during the ongoing viral outbreak, apparently as a result of their mothers being infected during pregnancy, since, in some cases, ZIKV-RNA has been detected in the amniotic fluid of the mothers<sup><xref ref-type="fn" rid="fn04">4</xref></sup>. Around 4000 cases of Zika-related microcephaly have been recorded in that country until now since October 2015, accounting for over 40 infants deaths (<xref rid="B64" ref-type="bibr">Higgs, 2016</xref>). Furthermore, ZIKV has been recently found in the fetal brain tissue of a baby with microcephaly after termination of the pregnancy requested by the infected woman (<xref rid="B94" ref-type="bibr">Mlakar et al., 2016</xref>).</p><p>At present (February 2016), three reported cases indicate that ZIKV could be sexually transmitted. In August 2008, a scientist was bitten many times while studying mosquitoes in south-eastern Senegal. Six days after returning to his home in Colorado (U.S.), he felt ill with symptoms of Zika fever and hematospermia. By then, he had had unprotected intercourse with his wife, who had not been outside the U.S. during the previous year, which subsequently developed symptoms of Zika fever. Virus infection was confirmed by serologic testing in both, but the presence of ZIKV in the semen of the patient was not investigated (<xref rid="B49" ref-type="bibr">Foy et al., 2011</xref>). A second report described the presence of replicative ZIKV and a high ZIKV RNA load in semen (1,1–2,9 × 10<sup>7</sup> copies/ml) and urine (3,8 × 10<sup>3</sup> copies/ml) samples of a patient during the 2013 outbreak in Tahiti, but no RT-PCR amplification was obtained from sera collected at the same time (<xref rid="B104" ref-type="bibr">Musso et al., 2015b</xref>). In early February 2016, the Dallas County Health and Human Services Department (U.S.) reported a third case, still under investigation, of a person that apparently contracted Zika fever after sexual contact with an ill person who had recently returned from a ZIKV high risk country<sup><xref ref-type="fn" rid="fn05">5</xref></sup>.</p><p>In addition to perinatal, breastfeeding, and sexual sporadic transmission of ZIKV, the potential for viral transmission through blood transfusion was demonstrated during the French Polynesian outbreak. Almost 3% (42/1505) of blood donors, who were asymptomatic at the time of donation, were found positive for acute ZIKV infection by specific RT-PCR (<xref rid="B101" ref-type="bibr">Musso et al., 2014a</xref>). Further studies are needed to assess the actual risk of ZIKV transmission through blood products and the risk to generate a disease in the recipient, but these data point to the necessity for quickly adapting blood donation safety procedures to the local epidemiological context. In fact, the Pan American Health Organization, PAHO<sup><xref ref-type="fn" rid="fn06">6</xref></sup>, and the European Centre for Disease Prevention and Controls, ECDC<sup><xref ref-type="fn" rid="fn07">7</xref></sup>, have recently issued a bulletins to alert their national health and blood safety authorities on this still poorly recognized viral infection.</p><p>Finally, ZIKV has been also detected in saliva samples (<xref rid="B16" ref-type="bibr">Besnard et al., 2014</xref>) with even higher frequency than in blood samples (<xref rid="B103" ref-type="bibr">Musso et al., 2015a</xref>) and, thus, saliva is another transmission source that have to be considered.</p><p>In resume, although ZIKV transmission by routes other than mosquito bites has been so far sporadic, further studies are mandatory to clearly establish their possible role on ZIKV epidemics.</p></sec></sec><sec><title>Ecology</title><p>Most arboviruses are perpetuated in transmission cycles independent of human hosts, but those with sylvatic cycles often infect people who accidentally intrude on their natural habitats. Nonetheless, in many instances, humans are dead-end hosts in complex transmission cycles that involve different wild and domestic vertebrate hosts, as in the case of JEV (<xref rid="B153" ref-type="bibr">Wolfe et al., 2001</xref>) or WNV (<xref rid="B88" ref-type="bibr">Martin-Acebes and Saiz, 2012</xref>). Less frequently, arboviruses may jump from this sylvatic transmission cycle to a mainly human-mosquito transmission cycle, as it likely was the case of CHIKV.</p><p>In the case of ZIKV, early studies indicated that non-human primates were the primary vertebrate hosts, with occasional participation of humans in the transmission cycle, even in highly enzootic areas. This theory was based on evidences indicating that <italic>A. africanus</italic>, a species with a greater preference for them than for humans (<xref rid="B59" ref-type="bibr">Haddow and Dick, 1948</xref>; <xref rid="B60" ref-type="bibr">Haddow et al., 1964</xref>), was the principal (if not the only) ZIKV vector (<xref rid="B60" ref-type="bibr">Haddow et al., 1964</xref>). However, by now, it seems clear that, as commented before, <italic>A. aegypti</italic> and, to a lesser extent, <italic>A. albopticus</italic> are the main vectors and, thus, that humans probably serve as primary amplification hosts when their viremia is sufficient in duration and magnitude (<xref rid="B58" ref-type="bibr">Haddow et al., 2012</xref>).</p><p>Although it is still unknown if ZIKV overwinters in geographical areas without reports of cases, it appears that, as in other flaviviral infections, such as that caused by WNV (<xref rid="B88" ref-type="bibr">Martin-Acebes and Saiz, 2012</xref>), epidemics are more related to the specific mosquito species, its population density, competence, and behavior in a given area, as this can shape virus dynamics.</p><p><italic>Aedes aegypti</italic>, which, as mentioned before, is found throughout Asia, Oceania, the Americas and in some regions of Africa and Europe<sup>2</sup> (<xref rid="B69" ref-type="bibr">Kraemer et al., 2015</xref>), does not overwinter, but can be sheltered in domestic settings, which provides protection against environmental conditions and numerous aquatic habitats suitable for oviposition. Beside Georgia, this species does not currently circulate in Europe, but there are no climatic reasons to believe that, if re-introduced, it cannot become widely established in southern Europe as before (<xref rid="B118" ref-type="bibr">Reiter, 2010</xref>).</p><p>Meanwhile, <italic>A. albopictus</italic>, the most invasive mosquito species in the world (<xref rid="B92" ref-type="bibr">Medlock et al., 2012</xref>), have spread during the last 30–40 years to North, Central and South America, parts of Africa, southeastern Asia, China, Japan, northern Australia, and southern Europe (<xref rid="B110" ref-type="bibr">Paupy et al., 2009</xref>). This successfully colonization of new regions is due to its ability to adapt to different climates through the production of cold-resistant eggs, with temperate strains surviving cold winters in northern latitudes. On top of that, its preference for container habitats (e.g., tires and vases) in domestic settings has resulted in increased potential for contact with humans (<xref rid="B92" ref-type="bibr">Medlock et al., 2012</xref>).</p><p>ZIKV pandemic is currently in progress, with many important questions still unanswered. However, as seen during recent years with other viral agents, urban overcrowding, constant international travel, disruption of the ecologic balance, and climate changes can favored the unexpected emergence of asleep, or yet unknown, infectious agents (<xref rid="B44" ref-type="bibr">Fauci and Morens, 2016</xref>). Therefore, comprehensive and integrated investigations have to be conducted to better understand the complex ecosystems in which agents of current and future pandemics could be aggressively evolving.</p></sec><sec><title>Epidemiology</title><sec><title>Africa</title><p>Since the first isolation of ZIKV in 1954 from an inhabitant of Nigeria (<xref rid="B83" ref-type="bibr">Macnamara, 1954</xref>), many serological and entomological studies have reported the circulation of the virus across a widespread area of Africa, including Kenya (<xref rid="B52" ref-type="bibr">Geser et al., 1970</xref>), Nigeria (<xref rid="B77" ref-type="bibr">Lee and Moore, 1972</xref>; <xref rid="B96" ref-type="bibr">Monath et al., 1973</xref>; <xref rid="B43" ref-type="bibr">Fagbami, 1979</xref>), Sierra Leone (<xref rid="B120" ref-type="bibr">Robin and Mouchet, 1975</xref>), Gabon (<xref rid="B67" ref-type="bibr">Jan et al., 1978</xref>; <xref rid="B57" ref-type="bibr">Grard et al., 2014</xref>), Uganda (<xref rid="B91" ref-type="bibr">McCrae and Kirya, 1982</xref>), Central African Republic (<xref rid="B125" ref-type="bibr">Saluzzo et al., 1981</xref>), Senegal (<xref rid="B97" ref-type="bibr">Monlun et al., 1993</xref>; <xref rid="B34" ref-type="bibr">Diallo et al., 2014</xref>; <xref rid="B5" ref-type="bibr">Althouse et al., 2015</xref>), and Ivory Coast (<xref rid="B3" ref-type="bibr">Akoua-Koffi et al., 2001</xref>), with prevalence ranging 1.3–52%. At present, Cape Verde is the only African country where, since last year, active viral transmission is currently being reported<sup><xref ref-type="fn" rid="fn08">8</xref></sup>,<sup><xref ref-type="fn" rid="fn09">9</xref></sup> (<bold>Figure <xref ref-type="fig" rid="F5">5</xref></bold>). From the beginning of the outbreak in October 2015 to 7th February of this year, the Health Authorities have reported 7362 cases without associated neurological disorders<sup><xref ref-type="fn" rid="fn010">10</xref></sup>. In any case, and although until now human cases of ZIKV related disease have only been sporadically documented in Africa, it should be kept in mind that this might have been partially due to underdiagnoses, mainly in areas where DENV and CHIKV circulate, as infection with all these viruses presents similar clinical signs (<xref rid="B150" ref-type="bibr">Weissenbock et al., 2010</xref>; <xref rid="B57" ref-type="bibr">Grard et al., 2014</xref>).</p><fig id="F5" position="float"><label>FIGURE 5</label><caption><p><bold>Map showing worldwide autochthonous and imported ZIKV human cases since the last 9 months.</bold> See text for details.</p></caption><graphic xlink:href="fmicb-07-00496-g005"></graphic></fig></sec><sec><title>Asia</title><p>In Asia, distinguishing ZIKV infection from other arboviral infections (dengue, yellow fever, and other tropical diseases) is also difficult, so that, early epidemiological data should be treated with caution. In the early 50’s, seroprevalence of ZIKV in humans was reported in several countries: India, Malaysia, Philippines, Vietnam, and Thailand with variable rates, 8–75% (<xref rid="B132" ref-type="bibr">Smithburn, 1954</xref>; <xref rid="B133" ref-type="bibr">Smithburn et al., 1954</xref>; <xref rid="B62" ref-type="bibr">Hammon et al., 1958</xref>; <xref rid="B114" ref-type="bibr">Pond, 1963</xref>; <xref rid="B85" ref-type="bibr">Marchette et al., 1969</xref>). As the time went by, other countries reported seroprevalence in humans: Indonesia (Java island) from 1977 to 1978 (<xref rid="B109" ref-type="bibr">Olson et al., 1981</xref>, <xref rid="B108" ref-type="bibr">1983</xref>) and Pakistan in 1980 (<xref rid="B27" ref-type="bibr">Darwish et al., 1983</xref>). More recently a few sporadic human cases have been documented: a confirmed case in Cambodia in 2010 (<xref rid="B63" ref-type="bibr">Heang et al., 2012</xref>), an infected traveler who returned from Indonesia in 2013 (<xref rid="B74" ref-type="bibr">Kwong et al., 2013</xref>), and some several ZIKV positive cases from travelers returning from Thailand during 2012–2014 (<xref rid="B19" ref-type="bibr">Buathong et al., 2015</xref>). Very recently, in January 2016, WHO notified a case of ZIKV infection in a traveler coming back to Finland after spending a few months in the Maldives<sup><xref ref-type="fn" rid="fn011">11</xref></sup> (<bold>Figure <xref ref-type="fig" rid="F5">5</xref></bold>).</p></sec><sec><title>Oceania</title><p>In April 2007, a ZIKV outbreak was reported in Yap Island, Micronesia. The way through the virus was introduced is still unknown, but, as commented before, it has been proposed it was due to an infected mosquito or to an asymptomatic person with undetected infection. Serological analysis indicated that 73% of Yap residents had been infected with ZIKV, but not a single hospitalization, hemorrhagic manifestation, or death were reported during the outbreak (<xref rid="B39" ref-type="bibr">Duffy et al., 2009</xref>).</p><p>After detection of the first case of ZIKV infection in the French Polynesia in October 2013, during the following outbreak, up to an estimated 11% of population was affected (<xref rid="B24" ref-type="bibr">Cao-Lormeau et al., 2014</xref>; <xref rid="B102" ref-type="bibr">Musso et al., 2014b</xref>). Most of the clinical cases presented low fever, asthenia, wrist and fingers arthralgia, headache, rash, and only one patient presented Guillain–Barre syndrome (GBS) 7 days after laboratory confirmation of ZIKV infection. It is noteworthy to remark that, after this outbreak, the incidence of GBS in French Polynesia increased 20-fold (<xref rid="B106" ref-type="bibr">Oehler et al., 2014</xref>). The reasons for this increase are not known yet. Analyses of the circulating virus have shown that it was genetically closely related to the 2007 Yap and the 2010 Camboya strains, which were not associated to a noticeable number of GBS cases.</p><p>Following the French Polynesia outbreak in late 2013, subsequent outbreaks occurred in New Caledonia, Eastern Island, and the Cook Islands. In New Caledonia, the first cases of ZIKV infection were imported from French Polynesia in November 2013, and, subsequently, in January 2014, the first autochthonous case was documented, driving the New Caledonia Health Authority to declare an outbreak situation in February 2014. Up to 1385 laboratory confirmed cases were reported (<xref rid="B40" ref-type="bibr">Dupont-Rouzeyrol et al., 2015</xref>). The outbreak in Eastern Island also started in January 2014, accounting for 51 confirmed cases (<xref rid="B140" ref-type="bibr">Tognarelli et al., 2016</xref>).</p><p>In Australia, the first case of ZIKV infection was notified in 2012 in a traveler returning from Indonesia (<xref rid="B74" ref-type="bibr">Kwong et al., 2013</xref>). Since then, all cases have been imported from countries affected by the virus. Nonetheless, as <italic>A. aegypti</italic>, the main ZIKV vector, is present mainly in areas of North Queensland, this region is at risk of an eventual local transmission of ZIKV from infected returning travelers<sup><xref ref-type="fn" rid="fn012">12</xref></sup>. A few imported cases have been confirmed in New Zealand too, but as the mosquito vectors are not commonly found in these territories, the risk of local transmission is so far low<sup><xref ref-type="fn" rid="fn013">13</xref></sup>.</p><p>In the past 9 months, Fiji, New Caledonia, Vanuatu, Solomon Islands, Marshall Islands, American Samoa, Samoa, and Tonga (<bold>Figure <xref ref-type="fig" rid="F5">5</xref></bold>) have reported autochthonous cases<sup>8,9</sup> but only the latter three have ongoing outbreaks<sup><xref ref-type="fn" rid="fn014">14</xref></sup>.</p></sec><sec><title>America</title><p>At the beginning of 2015, first autochthonous transmission of ZIKV in Brazil was reported and, since then, the virus has rapidly spread throughout the Americas (<xref rid="B156" ref-type="bibr">Zanluca et al., 2015</xref>). The Brazilian Ministry of Health estimates that between 440 000 to 1 300 000 cases of ZIKV infections may have occurred in 2015 in the country<sup><xref ref-type="fn" rid="fn015">15</xref></sup>.</p><p>Since the initial report in Brazil, during the last 9 months, and as a 18th of February 2016, 29 countries, or territories, of America have also reported autochthonous cases of ZIKV infection: Aruba, Barbados, Bolivia, Bonaire, Brazil, Colombia, Costa Rica, Curaçao, Dominican Republic, Ecuador, El Salvador, French Guyana, Guadeloupe, Guatemala, Guyana, Haiti, Honduras, Jamaica, Martinique, Mexico, Nicaragua, Panama, Paraguay, Puerto Rico, Saint Martin, Suriname, Trinidad y Tobago, Venezuela, and Virgin Islands<sup>8</sup> (<bold>Figure <xref ref-type="fig" rid="F5">5</xref></bold>). Because the epidemic is still spreading in the Americas, it is reasonable to think that more countries will report autochthonous ZIKV infections in the coming months.</p><p>Despite outbreaks have occurred mainly in Caribbean countries/territories and central and south America, imported confirmed cases are starting to show in North America: USA and Canada (<bold>Figure <xref ref-type="fig" rid="F5">5</xref></bold>). As of today, 82 and 3 imported cases have been reported in these countries, respectively<sup><xref ref-type="fn" rid="fn016">16</xref></sup><sup>,</sup><sup><xref ref-type="fn" rid="fn017">17</xref></sup>. Although the risk of ZIKV establishment in Canada and northern USA is low because of the absence of the vectors, it cannot be discarded that the virus persists and spreads in these regions causing autochthonous infected human cases, as it has happened with other related flaviviruses like WNV (<xref rid="B88" ref-type="bibr">Martin-Acebes and Saiz, 2012</xref>).</p></sec><sec><title>Europe</title><p>Nowadays, there is no evidence of autochthonous ZIKV infection in Europe. All reported cases were imported from people returning from affected countries. The first laboratory confirmed case was notified in November 2013 in Germany (<xref rid="B137" ref-type="bibr">Tappe et al., 2014</xref>). Since then, from 2015, imported cases have been documented in Norway (<xref rid="B147" ref-type="bibr">Waehre et al., 2014</xref>), Italy (<xref rid="B155" ref-type="bibr">Zammarchi et al., 2015</xref>), and Germany (<xref rid="B136" ref-type="bibr">Tappe et al., 2015</xref>). However, after the recent explosive expansion of the virus in the Americas, many European countries have reported imported cases of ZIKV infection in travelers returning home (<bold>Figure <xref ref-type="fig" rid="F5">5</xref></bold>), including Austria, Denmark, Finland, France, Germany, Ireland, Italy, Portugal, the Netherlands, Spain, Slovenia, Sweden, Switzerland, and UK<sup><xref ref-type="fn" rid="fn018">18</xref></sup>.</p><p>As commented above, currently only <italic>A. albopictus</italic> has colonized Europe, mainly the Mediterranean area (<xref rid="B92" ref-type="bibr">Medlock et al., 2012</xref>; <xref rid="B69" ref-type="bibr">Kraemer et al., 2015</xref>). Since <italic>A. aegypti</italic> is the main responsible of the current transmission of ZIKV in other regions of the world, the actual risk of a ZIKV outbreak in Europe seems to be low. Nonetheless, beside the possible reintroduction of <italic>A. aegypti</italic> in the continent, <italic>A. albopticus</italic> is circulating in the southern regions of the continent and, since this species can also efficiently transmit the virus, surveillance programs should be implemented, mainly during warm seasons. In fact, autochthonous DENV and CHIKV infections in France (<xref rid="B75" ref-type="bibr">La Ruche et al., 2010</xref>; <xref rid="B31" ref-type="bibr">Delisle et al., 2015</xref>), Croatia (<xref rid="B53" ref-type="bibr">Gjenero-Margan et al., 2011</xref>), and Italy (<xref rid="B119" ref-type="bibr">Rezza et al., 2007</xref>) have already been documented.</p></sec></sec><sec><title>Clinical Manifestations and Pathogeny</title><sec><title>Humans</title><p>Zika virus infection has been reported to be symptomatic only in around 18% of the cases (<xref rid="B39" ref-type="bibr">Duffy et al., 2009</xref>), in which it causes a mild, self-limiting disease with an incubation period of up to 10 days, often mistaken with other arboviral infections like dengue or chikungunya. Clinical manifestations in symptomatic cases resemble that of an influenza-like syndrome (<xref rid="B83" ref-type="bibr">Macnamara, 1954</xref>), being the most common symptoms fever, rash, arthralgia, and conjunctivitis; with headache, vomiting, edema, and jaundice being reported less frequently (<xref rid="B155" ref-type="bibr">Zammarchi et al., 2015</xref>). Digestive complications (abdominal pain, diarrhea, and constipation), mucous membrane ulcerations (aphthae), and pruritus are rarely observed. The symptoms usually resolve spontaneously after 3–7 days, but arthralgia may persist for up to 1 month (<xref rid="B49" ref-type="bibr">Foy et al., 2011</xref>). A post-infection asthenia seems to be also frequent. In any case, severe disease requiring hospitalization has been uncommon until now.</p><p>First description of clinical manifestations were reported in 1954 by Macnamara (<xref rid="B83" ref-type="bibr">Macnamara, 1954</xref>). In 1956, Bearcroft described the symptoms in an experimentally infected human volunteer (<xref rid="B11" ref-type="bibr">Bearcroft, 1956</xref>). A slight generalized headache and a rise in temperature started at the 3rd day, increasing during day 5. By day 7 the patient felt well and the temperature had fallen to normal. Jaundice did not develop, and no other evidence of hepatic dysfunction was found. The total white blood cells during the first 12 days after inoculation did not differ greatly from pre-inoculation counts. Urine estimations were consistently negative for bile pigments and albumin during this period. Estimations of total serum bilirubin carried out between the time of inoculation and the 17th day showed normal levels, and the site of inoculation appeared healthy.</p><p>In 1964, Simpson described his own acquired ZIKV illness while working with ZIKV strains isolated from <italic>A. africanus</italic> collected during 1962–1963 (<xref rid="B130" ref-type="bibr">Simpson, 1964</xref>). The illness began with a slight frontal headache, showing no other symptoms at the time. During the 2nd day he presented a diffuse pink maculopapular rash which covered the face, neck, trunk, and upper arms that spread gradually to involve all four limbs, and felt slight aching sensations in his back and thighs. Oral temperature at this time was normal in the morning, however he was slightly febrile throughout the day. The temperature returned to normal by the end of the day, and he felt much better, apart from a slight headache. The rash persisted to day 5, when it was fading until completely disappear. No other signs or symptoms were noted during the illness.</p><p>Information on laboratory tested alterations associated with ZIKV infection are limited, but may include transient leucopenia, with (<xref rid="B73" ref-type="bibr">Kutsuna et al., 2014</xref>) or without thrombocytopenia (<xref rid="B74" ref-type="bibr">Kwong et al., 2013</xref>), and slight elevation of serum lactate dehydrogenase, gamma-glutamyl transferase, and of inflammatory parameters (C– reactive protein, fibrinogen and ferritin) (<xref rid="B137" ref-type="bibr">Tappe et al., 2014</xref>). Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) concentrations may or may not be elevated.</p><sec><title>Guillain–Barre Syndrome (GBS)</title><p>An association of ZIKV infection with more severe disease outcomes, such as GBS has been also proposed. GBS is an autoimmune disease causing acute or subacute flaccid paralysis that can even cause death (<xref rid="B142" ref-type="bibr">van den Berg et al., 2013</xref>; <xref rid="B37" ref-type="bibr">Dominguez-Moreno et al., 2014</xref>), and that it has been previously associated with other flaviviral infections (<xref rid="B116" ref-type="bibr">Puccioni-Sohler et al., 2012</xref>). Remarkably, as aforementioned, during ZIKV outbreak reported in French Polynesia the incidence rate of GBS cases was approximately 20-fold higher than expected given the size of the French Polynesia population and its previously established incidence (1–2/100 000 population per year) (<xref rid="B106" ref-type="bibr">Oehler et al., 2014</xref>). Likewise, in Colombia, during the ongoing outbreak, 86 cases of GBS have been associated to ZIKV infection<sup><xref ref-type="fn" rid="fn019">19</xref></sup>. Based on the 600 000 expected Zika infections in the country, up to 1000 cases of GBS could be anticipated. These data point to a worrisome increase in the potential clinical severity of the disease (<xref rid="B123" ref-type="bibr">Roth et al., 2014</xref>).</p></sec><sec><title>Microcephaly</title><p>Similarly to GBS, even more disturbing is the astonishing rise in the number of babies born with microcephaly and neurological disorders that have been suggested to be associated with the current ZIKV outbreak in Brazil (<xref rid="B126" ref-type="bibr">Schuler-Faccini et al., 2016</xref>). These congenital infections presumably due to ZIKV exposure have been also associated with an increase in vision-threatening findings, which include bilateral macular and perimacular lesions, as well as optic nerve abnormalities in most cases (<xref rid="B29" ref-type="bibr">de Paula Freitas et al., 2016</xref>; <xref rid="B144" ref-type="bibr">Ventura et al., 2016a</xref>,<xref rid="B145" ref-type="bibr">b</xref>). By the end of 2015, Brazil’s Health Ministry reported an unusual spike in reported cases of microcephaly in the northeastern state of Pernambuco, where the affected children’s mothers had been in early pregnancy at around the same time as large ZIKV outbreaks occurred. The Ministry subsequently raised the alarm of a possible link to ZIKV. Although most cases have been described in Pernambuco, they have also been diagnosed in other eight Brazilian states so far (<xref rid="B107" ref-type="bibr">Oliveira Melo et al., 2016</xref>). However, it should be noted that neighboring Colombia has reported over 5000 cases of ZIKV in pregnant women and so far, only one documented case of microcephaly in a newborn, and other two with other congenital brain abnormalities have been very recently documented (<xref rid="B21" ref-type="bibr">Butler, 2016</xref>).</p><p>In any case, this possible association of microcephaly with ZIKV is still a matter of controversy among researchers. The Latin American Collaborative Study of Congenital Malformations (ECLAMC) suggested that the rise in reported cases of microcephaly might largely be attributable to the intense search for cases of the birth defect, and to misdiagnoses, that arose from heightened awareness in the wake of the possible link with ZIKV. However, it should be noted that a successful RT-PCR amplification of the complete ZIKV genome from a fetal brain tissue has been recently described (<xref rid="B94" ref-type="bibr">Mlakar et al., 2016</xref>). In this line, an expectant mother presented a febrile illness with rash at the end of the first trimester of pregnancy while she was living in Brazil. Ultrasonography performed at 29 weeks of gestation revealed microcephaly with calcifications in the fetal brain and placenta. After the mother requested termination of the pregnancy, the fetal autopsy revealed microcephaly, with almost complete agyria, hydrocephalus, and multifocal dystrophic calcifications in the cortex and subcortical white matter, with associated cortical displacement and mild focal inflammation. Electron microscopy analysis also revealed spherical virus particles with morphologic characteristics consistent with ZIKV. Likewise, very recently, the ZIKV genome has been detected and sequenced form amniotic fluid samples of two pregnant women whose fetuses where diagnosed with microcephaly, (<xref rid="B22" ref-type="bibr">Calvet et al., 2016</xref>).</p><p>The apparent risk of microcephaly was enough for the World Health Organization (WHO) to declare a public health emergency of international concern on February 1 (<xref rid="B124" ref-type="bibr">Rubin et al., 2016</xref>) that has lately been integrated into risk assessments by the ECDC<sup>4</sup>. Therefore, the most important current milestone in ZIKV investigations is to clearly elucidate the possible relationship of the infection with the development of serious neurological disorders.</p></sec></sec><sec><title>Animal Models</title><p>The pathogenicity of ZIKV has been also evaluated in several animal models. Among these, the most widely used has been the mouse model. Moreover, initial isolation of ZIKV was performed by intracerebral inoculation of viral samples into mice (<xref rid="B35" ref-type="bibr">Dick, 1952</xref>). On these initial experiments, infectious virus was only recovered from infected brain mice, whereas no infectious virus able to replicate when inoculated in other mice was recovered from non-nervous tissues such as kidney, lung, liver, or spleens, highlighting the marked neurotropism of ZIKV. Infected mice displayed detectable signs of infection about 5–6 days post-infection, time at which virus titers peaked (<xref rid="B35" ref-type="bibr">Dick, 1952</xref>). The analysis of the pathological changes observed in ZIKV-infected mice brains revealed various stages of cellular infiltration and degeneration that were also found in the spinal cords. Degeneration of nerve cells, especially in the region of the hippocampus, resulted in an early and marked enlargement of astroglial cells with patchy destruction of the pyriform cells of Ammon’s horn (<xref rid="B149" ref-type="bibr">Weinbren and Williams, 1958</xref>; <xref rid="B12" ref-type="bibr">Bell et al., 1971</xref>). Microscopy analysis confirmed that the virus replicates in both neurons and astrogial cells (<xref rid="B149" ref-type="bibr">Weinbren and Williams, 1958</xref>; <xref rid="B12" ref-type="bibr">Bell et al., 1971</xref>). Remarkably, while mice of all ages tested were susceptible to intracerebral inoculations, mice of 2 week of age and over could rarely be infected by the intraperitoneal route. In contrast, mice younger than 2 weeks were highly susceptible to intraperitoneal inoculation of the virus (<xref rid="B35" ref-type="bibr">Dick, 1952</xref>). This finding is similar to that reported for other flaviviruses, such as Usutu virus (<xref rid="B17" ref-type="bibr">Blazquez et al., 2015</xref>). ZIKV-induced pathological changes are confined to the central nervous system in older animals, but myocarditis (and associated pulmonary oedema) and skeletal myositis can be also found in young (1–5 days old) animals infected with ZIKV (<xref rid="B149" ref-type="bibr">Weinbren and Williams, 1958</xref>). However, it has to be considered that only non-mouse-adapted strain of ZIKV seems to induce myocarditis (<xref rid="B149" ref-type="bibr">Weinbren and Williams, 1958</xref>). Besides mice, the susceptibility to ZIKV has been also evaluated in other non-primate small animal models, demonstrating that cotton-rats, guinea pigs, and rabbits did not show clinical signs of infection after intracerebral inoculation of a late passage mouse brain virus. Nevertheless, inoculation of low passage ZIKV in guinea pigs resulted in death at 6 days post-infection (<xref rid="B35" ref-type="bibr">Dick, 1952</xref>).</p><p>The pathogenicity of ZIKV in monkeys seems to be also mild, except from the sentinel Rhesus 766 from which ZIKV was initially isolated that exhibited a slight pyrexia (<xref rid="B36" ref-type="bibr">Dick et al., 1952</xref>). Experimentally infected monkeys developed an unapparent infection after subcutaneous inoculation with mouse recovered ZIKV. Only one of five monkeys tested showed a mild pyrexia after intracerebral inoculation, whereas the others showed no signs of infection (<xref rid="B35" ref-type="bibr">Dick, 1952</xref>). In any case, it should be noted that all infected monkeys showed viremia during the 1st week after inoculation, as well as induction of specific antibodies about 14 days post-inoculation (<xref rid="B35" ref-type="bibr">Dick, 1952</xref>). This induction of viremia in monkeys is supposed to play an essential role for the establishment of the enzootic transmission cycle between non-human primates and mosquitoes.</p></sec></sec><sec><title>Diagnosis</title><p>Different arboviral infections can have similar clinical presentations and, thus, their circulation may be underreported if specific diagnostic tools have not been implemented. In the case of ZIKV, diagnosis presents several drawbacks; there is no “gold standard” diagnosis tool, antibodies are frequently cross-reactive between flaviviruses, which limits the use of serology, viral culture is not routinely performed, and, so far, there is no antigenic detection test available (<xref rid="B103" ref-type="bibr">Musso et al., 2015a</xref>). At present, the diagnosis of ZIKV infection is mainly made through “in house” molecular (RT-PCR) and serologic [Ig M ELISA and plaque reduction neutralization test (PRNT)] assays, as only very recently molecular commercial tests for ZIKV have been made available<sup><xref ref-type="fn" rid="fn020">20</xref></sup>,<sup><xref ref-type="fn" rid="fn021">21</xref></sup>.</p><sec><title>Antibody</title><p>An IgM ELISA was developed at the Arboviral Diagnostic and Reference Laboratory of the CDC to detect ZIKV using samples from Yap Island outbreak (<xref rid="B76" ref-type="bibr">Lanciotti et al., 2008</xref>). IgM was detectable as early as 3 days after onset of illness, but cross-reaction with other flaviviruses was observed. This cross-reactivity of sera from convalescent-phase patients was more frequent in those from patients with evidence of previous flavivirus infections than among those with apparent primary ZIKV infections, mainly in the case of previous DENV infection (<xref rid="B76" ref-type="bibr">Lanciotti et al., 2008</xref>; <xref rid="B39" ref-type="bibr">Duffy et al., 2009</xref>). This IgM cross-reactivity has been further corroborated (<xref rid="B155" ref-type="bibr">Zammarchi et al., 2015</xref>; <xref rid="B128" ref-type="bibr">Shinohara et al., 2016</xref>), so, as in many other flaviviral infections, ELISA positive results should be confirmed, either by testing an acute-phase serum sample collected as early as possible after onset of illness and a second sample collected 2–3 weeks later, or by additional assays, such as PRNT, that have to result in at least a fourfold increase in ZIKV neutralizing antibody titers when compared with that of the other viruses tested<sup><xref ref-type="fn" rid="fn022">22</xref></sup>.</p><p>Recently, a report analyzing the presence of IgG against different flaviviruses among blood donors in French Polynesia, based on the use of recombinant antigens comprising the domain III of the envelope protein of each virus strain, claimed to differentiate between them, as an overall seropositivity rates of 80.3% for at least one DENV serotype, 0.8% for ZIKV, 1.3% for JEV, and 1.5% for WNV were recorded (<xref rid="B8" ref-type="bibr">Aubry et al., 2015</xref>). In any case, it should be noticed that ELISA cross-reactivity can also be the result of co-infections with more than one flavivirus that co-circulate in the same regions, as recently reported for two cases (<xref rid="B40" ref-type="bibr">Dupont-Rouzeyrol et al., 2015</xref>).</p></sec><sec><title>Nucleic Acid</title><p>The diagnosis of ZIKV is at present primarily based on the detection of viral RNA from specimens by means of RT-PCR (<xref rid="B46" ref-type="bibr">Faye et al., 2008</xref>; <xref rid="B24" ref-type="bibr">Cao-Lormeau et al., 2014</xref>; <xref rid="B57" ref-type="bibr">Grard et al., 2014</xref>; <xref rid="B103" ref-type="bibr">Musso et al., 2015a</xref>; <xref rid="B155" ref-type="bibr">Zammarchi et al., 2015</xref>; <xref rid="B86" ref-type="bibr">Marcondes and Ximenes, 2016</xref>; <xref rid="B128" ref-type="bibr">Shinohara et al., 2016</xref>). However, as the viremic period is short, direct virus detection should be performed in samples took during the first 3–5 days after the onset of symptoms (<xref rid="B9" ref-type="bibr">Balm et al., 2012</xref>).</p><p>A specific ZIKV nucleic acid test (NAT) was implemented in routine practice during the French Polynesian outbreak (<xref rid="B101" ref-type="bibr">Musso et al., 2014a</xref>) on the basis of protocols implemented to prevent WNV transmission by transfusion in North America. Additional NATs have been developed based on specific Asian and African ZIKV strains targeting either the envelope or the NS5 regions (<xref rid="B39" ref-type="bibr">Duffy et al., 2009</xref>; <xref rid="B45" ref-type="bibr">Faye et al., 2013</xref>). Likewise, a one-step RT-PCR assay to detect ZIKV in human serum has also been developed (<xref rid="B46" ref-type="bibr">Faye et al., 2008</xref>). The assay showed to be rapid, sensitive, and specific to detect ZIKV in cell culture or serum. Nevertheless, since experimentally infected samples were used, it needs to be validated for diagnosis using clinical samples.</p><p>All these NATs have several limitations. Because of its nucleotide sequence specificity, NAT cannot be used to screen a wide range of pathogens with one run, being necessary the use of multiple assays if several pathogens are co-circulating in the same area, but multiple testing is expensive and time-consuming (<xref rid="B7" ref-type="bibr">Aubry et al., 2016</xref>), and it does not detect all infected blood donations, especially when nucleic acid loads are low and when sera are tested in large pools (<xref rid="B103" ref-type="bibr">Musso et al., 2015a</xref>). Therefore, use of alternative source of samples has been proposed. In this sense, the suitability of urine samples for diagnosing ZIKV infection has been recently confirmed, as RNA of the virus is detectable in urine at a higher load and with a longer duration than in serum (10 to >20 days, and >7 days once it become undetectable in serum) (<xref rid="B56" ref-type="bibr">Gourinat et al., 2015</xref>; <xref rid="B128" ref-type="bibr">Shinohara et al., 2016</xref>). Saliva has also been used as an alternative sample for routine ZIKV RNA detection, showing positivity more frequently than blood samples, but it did not increase the window of detection in contrast to what was reported for urine. Since ZIKV RNA detection was found negative in some saliva samples while positive in blood, saliva cannot replace blood samples, but just help on detection (<xref rid="B103" ref-type="bibr">Musso et al., 2015a</xref>). As a result of these data, it has been suggested to collect both blood and saliva samples to increase the sensitivity of molecular detection of viral RNA for Zika fever diagnosis in acute phase. On the other hand, urine sample can be associated at the late stages of the disease. Thus, when detection of ZIKV is of particular importance, using a combination of samples (blood/saliva/urine) is recommended (<xref rid="B103" ref-type="bibr">Musso et al., 2015a</xref>).</p></sec></sec><sec><title>Prophylaxis</title><p>Currently there are no specific antiviral agents, vaccine, or prophylaxis for ZIKV. Treatment is generally directed to symptom relief with analgesics and anti-pyretics. Therefore, developing an effective, safe, and affordable ZIKV vaccine, and search for antiviral effective compounds for disease treatment is a current challenge for Zika disease.</p><p>Vaccines for various flaviviruses have been produced during the past years, some of them being already in the market, such as those for YFV or WNV (<xref rid="B28" ref-type="bibr">Dauphin and Zientara, 2007</xref>; <xref rid="B141" ref-type="bibr">Ulbert and Magnusson, 2014</xref>; <xref rid="B95" ref-type="bibr">Monath and Vasconcelos, 2015</xref>). These vaccines have been produced using different strategies, inactivated or live-attenuated viruses, recombinant proteins or peptides expressed in different heterologous systems, recombinant subviral particles, chimeric backbone viruses, or naked cDNA (<xref rid="B66" ref-type="bibr">Ishikawa et al., 2014</xref>). Thus, it seems reasonable to think that similar strategies can be applied to ZIKV. For instances, a DNA based vaccine (SynCon, Phamaceutical, U.S.) has been recently produced, which it is expected to go into Phase I trials before the end of 2016. In addition, a global patent of two vaccine candidates (a recombinant vaccine and an inactivated vaccine) for ZIKV has been just filled (Bharat Biotech, India).</p><p>Developing effective specific therapies for ZIKV seems much more difficult, as so far, and despite several attempts have been made in the past years, none of such compounds are available against any flaviviral infection (<xref rid="B6" ref-type="bibr">Apte-Sengupta et al., 2014</xref>). Along with drugs, antibody mediated protection against ZIKV should also be addressed, as in other flaviviral infections (YF, WN, or dengue) experimental specific antibodies treatment has been some time successful (<xref rid="B122" ref-type="bibr">Roehrig et al., 2001</xref>; <xref rid="B13" ref-type="bibr">Ben-Nathan et al., 2003</xref>; <xref rid="B42" ref-type="bibr">Engle and Diamond, 2003</xref>).</p><p>Nevertheless, and despite the great effort that probably will be made within the scientific community in the coming years, it will take time until any drugs or vaccines against ZIKV will be commercially available.</p><sec><title>Public Health and Preventive Measures</title><p>As previously detailed, ZIKV infection generally causes a non-severe disease (<xref rid="B155" ref-type="bibr">Zammarchi et al., 2015</xref>), but some areas newly affected by the virus are providing worrisome information on the already mentioned potential association of neonatal malformations (microcephaly) and GBS with ZIKV (<xref rid="B106" ref-type="bibr">Oehler et al., 2014</xref>; <xref rid="B94" ref-type="bibr">Mlakar et al., 2016</xref>; <xref rid="B138" ref-type="bibr">Tetro, 2016</xref>). As a consequence, and in the absence of another explanation for these connections, the WHO declared a Public Health Emergency of International Concern on the 1st of February of 2016<sup><xref ref-type="fn" rid="fn023">23</xref></sup>, highlighting the importance of enhance the measures to reduce the ZIKV infection, particularly among pregnant women and women of childbearing age. In this way, as previously remarked, additional investigations are absolutely necessary to determine whether there is a casual link between ZIKV and microcephaly and GBS.</p><p>Currently, no vaccine or treatment exists to prevent ZIKV disease, although more probably several vaccine prototypes will be obtained soon. Thus, to facilitate the surveillance and control measures, research and development efforts should be intensified for ZIKV vaccines, therapeutics, and improved new diagnostics<sup>23</sup>, especially if the connection between ZIKV and microcephaly is confirmed.</p><p>In the meantime, prevention, and control of ZIKV infection are mainly focused on avoiding the bites of carrying mosquitoes responsible for disease transmission. These measures are the same as those recommended to prevent other diseases transmitted by mosquitoes bite (<xref rid="B88" ref-type="bibr">Martin-Acebes and Saiz, 2012</xref>), and include the use of insect repellent, wearing long-sleeved shirts and long pants, the elimination of standing water where mosquitoes can lay eggs, the minimization of outdoor activities coincident with the maximum activity of mosquitoes, the installation of window and door screens, and the implementation of accurate mosquito control programs<sup><xref ref-type="fn" rid="fn024">24</xref></sup>. ECDC and CDC recommend those pregnant women and those who are trying to get pregnant consider not travel to areas affected by the virus24,<sup><xref ref-type="fn" rid="fn025">25</xref></sup>. Furthermore, some American countries, including Colombia<sup><xref ref-type="fn" rid="fn026">26</xref></sup>, Honduras<sup><xref ref-type="fn" rid="fn027">27</xref></sup>, Ecuador<sup><xref ref-type="fn" rid="fn028">28</xref></sup>, El Salvador<sup><xref ref-type="fn" rid="fn029">29</xref></sup>, and Jamaica<sup><xref ref-type="fn" rid="fn030">30</xref></sup> have recommended delay pregnancy during the ZIKV outbreak.</p><p>On the other hand, in territories where autochthonous ZIKV infection has not yet been detected, but potential vectors are present, the public health institutions should have a quick response to prevent local transmission when an imported case is confirmed. In this context, WHO called for health authorities to collaborate with the transport sector to ensure disinfection of aircraft from affected areas<sup>23</sup>.</p><p>As described before, a few cases of sexual transmission of ZIKV has been reported (<xref rid="B104" ref-type="bibr">Musso et al., 2015b</xref>; <xref rid="B90" ref-type="bibr">McCarthy, 2016</xref>). Even though further studies are needed to confirm the actual sexual risk of ZIKV transmission, the CDC recommend the proper use of preventive measures, such as condoms, if having sex (vaginal, anal, or oral) with a male partner while traveling, or if he has just comeback from an area where the virus is actively circulating; even more, in this latter case, sexual abstinence is recommended if the partner is pregnant<sup><xref ref-type="fn" rid="fn031">31</xref></sup>.</p><p>Likewise, and since spread of the virus through blood transfusion has been reported (<xref rid="B101" ref-type="bibr">Musso et al., 2014a</xref>), the countries affected by the virus should take measures to prevent this way of infection (<xref rid="B84" ref-type="bibr">Marano et al., 2016</xref>). Moreover, in territories free of ZIKV, people returning from affected areas should delay blood donations<sup><xref ref-type="fn" rid="fn032">32</xref></sup>.</p><p>The rapid spread of ZIKV in Brazil and the Americas, with the suspected neonatal malformations associated, is being cause for alarm and the mass media are even questioning if the 2016 Olympic Games in Rio should be delayed or even canceled. In fact, the Brazilian authorities are performing intense vector control programs to eliminate mosquito vector that transmit ZIKV and other arboviruses (<xref rid="B111" ref-type="bibr">Petersen et al., 2016</xref>).</p></sec></sec><sec><title>Final Remarks</title><p>After the discovery of ZIKV in 1947, several investigations were performed during the 1950s and 1960s to characterize the new pathogen but, since then, little more was done until the spread of the virus to the Micronesia in 2007. After that, with the explosive invasion of the Americas in 2015, and the possible association of ZIKV with severe neurological diseases, these investigations have significantly increased, and it should be expected that much more information regarding the biology and the clinical consequences of ZIKV infection will be available soon. Several issues have to be resolved. For instance, most of the virus molecular biology and the virus-host cell interactions have been inferred from other related flaviviruses, and, thus, they should be specifically analyzed. Likewise, the elucidation of the presence of determinants of virulence/pathogenicity, and the role that protein glycosylations can play on it will be of much interest. On the other hand, the role of humans in the spread of ZIKV, and whether vertical and sexual transmission routes are important factors for human-to-human spread also need to be clearly determined. No least is the improvement of more accurate diagnostic tools, the development of vaccines, and the design of antiviral therapies. Finally, without any doubt, currently the most important and pressing issue is to reveal whether ZIKV infection is the cause of the increase in the number of GBS and microcephaly cases lately reported.</p></sec><sec><title>Author Contributions</title><p>All authors listed, have made substantial, direct and intellectual contribution to the work, and approved it for publication.</p></sec><sec><title>Conflict of Interest Statement</title><p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p></sec></body><back><fn-group><fn fn-type="financial-disclosure"><p><bold>Funding.</bold> This work was supported by grant ZIKA-BIO from INIA to J-CS, and AGL2014-56518-JIN from MINECO to MM-A, AV-C is a recipient of a “Contrato de formación postodoctoral” from MINECO. TM-R is a recipient of a “Formación de Personal Investigador (FPI)” pre-doctoral fellowship from INIA.</p></fn></fn-group><fn-group><fn id="fn01"><label>1</label><p><ext-link ext-link-type="uri" xlink:href="http://www.ictvonline.org/virustaxonomy.asp">http://www.ictvonline.org/virustaxonomy.asp</ext-link></p></fn><fn id="fn02"><label>2</label><p><ext-link ext-link-type="uri" xlink:href="http://ecdc.europa.eu/en/healthtopics/vectors/vector-maps/Pages/VBORNET_maps.aspx">http://ecdc.europa.eu/en/healthtopics/vectors/vector-maps/Pages/VBORNET_maps.aspx</ext-link></p></fn><fn id="fn03"><label>3</label><p><ext-link ext-link-type="uri" xlink:href="http://ecdc.europa.eu/en/healthtopics/zika_virus_infection/factsheet-health-professionals/Pages/factsheet_health_professionals.aspx">http://ecdc.europa.eu/en/healthtopics/zika_virus_infection/factsheet-health-professionals/Pages/factsheet_health_professionals.aspx</ext-link></p></fn><fn id="fn04"><label>4</label><p><ext-link ext-link-type="uri" xlink:href="http://ecdc.europa.eu/en/publications/Publications/rapid-risk-assessment-zika-virus-first-update-jan-2016.pdf">http://ecdc.europa.eu/en/publications/Publications/rapid-risk-assessment-zika-virus-first-update-jan-2016.pdf</ext-link></p></fn><fn id="fn05"><label>5</label><p><ext-link ext-link-type="uri" xlink:href="http://www.dallascounty.org/department/hhs/press/documents/PR2-2-16DCHHSReportsFirstCaseofZikaVirusThroughSexualTransmission.pdf">http://www.dallascounty.org/department/hhs/press/documents/PR2-2-16DCHHSReportsFirstCaseofZikaVirusThroughSexualTransmission.pdf</ext-link></p></fn><fn id="fn06"><label>6</label><p><ext-link ext-link-type="uri" xlink:href="http://www.paho.org/hq/index.php?option=com_content&view=article&id=11605:2016-paho-statement-on-zika-transmission-prevention-&Itemid=41716&lang=en">http://www.paho.org/hq/index.php?option=com_content&view=article&id=11605:2016-paho-statement-on-zika-transmission-prevention-&Itemid=41716&lang=en</ext-link></p></fn><fn id="fn07"><label>7</label><p><ext-link ext-link-type="uri" xlink:href="http://ecdc.europa.eu/en/press/news/_layouts/forms/News_DispForm.aspx?List=8db7286c-fe2d-476c-9133-18ff4cb1b568&ID=1348">http://ecdc.europa.eu/en/press/news/_layouts/forms/News_DispForm.aspx?List=8db7286c-fe2d-476c-9133-18ff4cb1b568&ID=1348</ext-link></p></fn><fn id="fn08"><label>8</label><p><ext-link ext-link-type="uri" xlink:href="http://ecdc.europa.eu/en/healthtopics/zika_virus_infection/zika-outbreak/Pages/Zika-countries-with-transmission.aspx">http://ecdc.europa.eu/en/healthtopics/zika_virus_infection/zika-outbreak/Pages/Zika-countries-with-transmission.aspx</ext-link></p></fn><fn id="fn09"><label>9</label><p><ext-link ext-link-type="uri" xlink:href="http://www.cdc.gov/zika/geo/active-countries.html">http://www.cdc.gov/zika/geo/active-countries.html</ext-link></p></fn><fn id="fn010"><label>10</label><p><ext-link ext-link-type="uri" xlink:href="http://www.minsaude.gov.cv/index.php/documentos/cat_view/34-documentacao/72-zika-virus">http://www.minsaude.gov.cv/index.php/documentos/cat_view/34-documentacao/72-zika-virus</ext-link></p></fn><fn id="fn011"><label>11</label><p><ext-link ext-link-type="uri" xlink:href="http://www.who.int/csr/don/8-february-2016-zika-maldives/en/">http://www.who.int/csr/don/8-february-2016-zika-maldives/en/</ext-link></p></fn><fn id="fn012"><label>12</label><p><ext-link ext-link-type="uri" xlink:href="http://www.health.gov.au/internet/main/publishing.nsf/Content/ohp-zika-health-practitioners.htm">http://www.health.gov.au/internet/main/publishing.nsf/Content/ohp-zika-health-practitioners.htm</ext-link></p></fn><fn id="fn013"><label>13</label><p><ext-link ext-link-type="uri" xlink:href="http://www.arphs.govt.nz/health-information/communicable-disease/dengue-fever-zika-chikungunya#.VsLrOyuG-WM">http://www.arphs.govt.nz/health-information/communicable-disease/dengue-fever-zika-chikungunya#.VsLrOyuG-WM</ext-link></p></fn><fn id="fn014"><label>14</label><p><ext-link ext-link-type="uri" xlink:href="http://smartraveller.gov.au/bulletins/zika_virus">http://smartraveller.gov.au/bulletins/zika_virus</ext-link></p></fn><fn id="fn015"><label>15</label><p><ext-link ext-link-type="uri" xlink:href="http://portalsaude.saude.gov.br/images/pdf/2015/dezembro/09/Microcefalia---Protocolo-de-vigil--ncia-eresposta---vers--o-1----09dez2015-8h.pdf">http://portalsaude.saude.gov.br/images/pdf/2015/dezembro/09/Microcefalia---Protocolo-de-vigil--ncia-eresposta---vers--o-1----09dez2015-8h.pdf</ext-link></p></fn><fn id="fn016"><label>16</label><p><ext-link ext-link-type="uri" xlink:href="http://www.cdc.gov/zika/geo/united-states.html">http://www.cdc.gov/zika/geo/united-states.html</ext-link></p></fn><fn id="fn017"><label>17</label><p><ext-link ext-link-type="uri" xlink:href="http://www.healthycanadians.gc.ca/publications/diseases-conditions-maladies-affections/risks-zika-virus-risques/index-eng.php">http://www.healthycanadians.gc.ca/publications/diseases-conditions-maladies-affections/risks-zika-virus-risques/index-eng.php</ext-link></p></fn><fn id="fn018"><label>18</label><p><ext-link ext-link-type="uri" xlink:href="http://ecdc.europa.eu/en/healthtopics/zika_virus_infection/zika-outbreak/Pages/epidemiological-situation.aspx">http://ecdc.europa.eu/en/healthtopics/zika_virus_infection/zika-outbreak/Pages/epidemiological-situation.aspx</ext-link></p></fn><fn id="fn019"><label>19</label><p><ext-link ext-link-type="uri" xlink:href="http://www.who.int/csr/don/12-february-2016-gbs-colombia-venezuela/en/">http://www.who.int/csr/don/12-february-2016-gbs-colombia-venezuela/en/</ext-link></p></fn><fn id="fn020"><label>20</label><p><ext-link ext-link-type="uri" xlink:href="http://www.euroimmun.co.uk/recent-news/first-commercial-antibody-tests-for-zika-virus-diagnostics">http://www.euroimmun.co.uk/recent-news/first-commercial-antibody-tests-for-zika-virus-diagnostics</ext-link></p></fn><fn id="fn021"><label>21</label><p><ext-link ext-link-type="uri" xlink:href="http://www.genesig.com/assets/files/zikv.pdf">www.genesig.com/assets/files/zikv.pdf</ext-link></p></fn><fn id="fn022"><label>22</label><p><ext-link ext-link-type="uri" xlink:href="http://www.cdc.gov/mmwr/volumes/65/wr/mm6503e3.htm">http://www.cdc.gov/mmwr/volumes/65/wr/mm6503e3.htm</ext-link></p></fn><fn id="fn023"><label>23</label><p><ext-link ext-link-type="uri" xlink:href="http://www.who.int/mediacentre/news/statements/2016/1st-emergency-committee-zika/en/">http://www.who.int/mediacentre/news/statements/2016/1st-emergency-committee-zika/en/</ext-link></p></fn><fn id="fn024"><label>24</label><p><ext-link ext-link-type="uri" xlink:href="http://www.cdc.gov/zika/prevention/index.html">http://www.cdc.gov/zika/prevention/index.html</ext-link></p></fn><fn id="fn025"><label>25</label><p><ext-link ext-link-type="uri" xlink:href="http://ecdc.europa.eu/en/healthtopics/zika_virus_infection/factsheet-health-professionals/Pages/factsheet_health_professionals.aspx">http://ecdc.europa.eu/en/healthtopics/zika_virus_infection/factsheet-health-professionals/Pages/factsheet_health_professionals.aspx</ext-link></p></fn><fn id="fn026"><label>26</label><p><ext-link ext-link-type="uri" xlink:href="https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/DE/DIJ/Circular-02-de-2016.pdf">https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/DE/DIJ/Circular-02-de-2016.pdf</ext-link></p></fn><fn id="fn027"><label>27</label><p><ext-link ext-link-type="uri" xlink:href="http://www.salud.gob.hn/">http://www.salud.gob.hn/</ext-link></p></fn><fn id="fn028"><label>28</label><p><ext-link ext-link-type="uri" xlink:href="http://www.salud.gob.ec/ministerio-de-salud-refuerza-recomendaciones-a-mujeres-embarazadas-por-virus-zika/">http://www.salud.gob.ec/ministerio-de-salud-refuerza-recomendaciones-a-mujeres-embarazadas-por-virus-zika/</ext-link></p></fn><fn id="fn029"><label>29</label><p><ext-link ext-link-type="uri" xlink:href="http://www.salud.gob.sv/novedades/noticias/noticias-ciudadanosas/348-enero-2016/3275--25-01-2016-sistema-nacional-de-proteccion-civil-realiza-jornada-municipal-contra-zancudo-transmisor-del-dengue-chikv-y-zika.html">http://www.salud.gob.sv/novedades/noticias/noticias-ciudadanosas/348-enero-2016/3275--25-01-2016-sistema-nacional-de-proteccion-civil-realiza-jornada-municipal-contra-zancudo-transmisor-del-dengue-chikv-y-zika.html</ext-link></p></fn><fn id="fn030"><label>30</label><p><ext-link ext-link-type="uri" xlink:href="http://moh.gov.jm/presentation/notes-for-minister-of-health-hon-horace-dalley-post-cabinet-press-briefing-january-20-2016-at-11a-m-office-of-the-prime-minister/">http://moh.gov.jm/presentation/notes-for-minister-of-health-hon-horace-dalley-post-cabinet-press-briefing-january-20-2016-at-11a-m-office-of-the-prime-minister/</ext-link></p></fn><fn id="fn031"><label>31</label><p><ext-link ext-link-type="uri" xlink:href="http://wwwnc.cdc.gov/travel/notices/alert/zika-virus-central-america">http://wwwnc.cdc.gov/travel/notices/alert/zika-virus-central-america</ext-link></p></fn><fn id="fn032"><label>32</label><p><ext-link ext-link-type="uri" xlink:href="http://www.msssi.gob.es/gabinete/notasPrensa.do?id=3900">http://www.msssi.gob.es/gabinete/notasPrensa.do?id=3900</ext-link></p></fn></fn-group><ref-list><title>References</title><ref id="B1"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Acosta</surname><given-names>E. G.</given-names></name><name><surname>Kumar</surname><given-names>A.</given-names></name><name><surname>Bartenschlager</surname><given-names>R.</given-names></name></person-group> (<year>2014</year>). <article-title>Revisiting dengue virus-host cell interaction: new insights into molecular and cellular virology.</article-title><source><italic>Adv. Virus Res.</italic></source><volume>88</volume><fpage>1</fpage>–<lpage>109</lpage>. <pub-id pub-id-type="doi">10.1016/B978-0-12-800098-4.00001-5</pub-id><pub-id pub-id-type="pmid">24373310</pub-id></mixed-citation></ref><ref id="B2"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Adams</surname><given-names>S. C.</given-names></name><name><surname>Broom</surname><given-names>A. K.</given-names></name><name><surname>Sammels</surname><given-names>L. M.</given-names></name><name><surname>Hartnett</surname><given-names>A. C.</given-names></name><name><surname>Howard</surname><given-names>M. J.</given-names></name><name><surname>Coelen</surname><given-names>R. J.</given-names></name><etal></etal></person-group> (<year>1995</year>). <article-title>Glycosylation and antigenic variation among Kunjin virus isolates.</article-title><source><italic>Virology</italic></source><volume>206</volume><fpage>49</fpage>–<lpage>56</lpage>. <pub-id pub-id-type="doi">10.1016/S0042-6822(95)80018-2</pub-id><pub-id pub-id-type="pmid">7530394</pub-id></mixed-citation></ref><ref id="B3"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Akoua-Koffi</surname><given-names>C.</given-names></name><name><surname>Diarrassouba</surname><given-names>S.</given-names></name><name><surname>Benie</surname><given-names>V. B.</given-names></name><name><surname>Ngbichi</surname><given-names>J. M.</given-names></name><name><surname>Bozoua</surname><given-names>T.</given-names></name><name><surname>Bosson</surname><given-names>A.</given-names></name><etal></etal></person-group> (<year>2001</year>). <article-title>[Investigation surrounding a fatal case of yellow fever in Cote d’Ivoire in 1999].</article-title><source><italic>Bull. Soc. Pathol. Exot.</italic></source><volume>94</volume><fpage>227</fpage>–<lpage>230</lpage>.<pub-id pub-id-type="pmid">11681215</pub-id></mixed-citation></ref><ref id="B4"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Alera</surname><given-names>M. T.</given-names></name><name><surname>Hermann</surname><given-names>L.</given-names></name><name><surname>Tac-An</surname><given-names>I. A.</given-names></name><name><surname>Klungthong</surname><given-names>C.</given-names></name><name><surname>Rutvisuttinunt</surname><given-names>W.</given-names></name><name><surname>Manasatienkij</surname><given-names>W.</given-names></name><etal></etal></person-group> (<year>2015</year>). <article-title>Zika virus infection, Philippines, 2012.</article-title><source><italic>Emerg. Infect. Dis.</italic></source><volume>21</volume><fpage>722</fpage>–<lpage>724</lpage>. <pub-id pub-id-type="doi">10.3201/eid2104.141707</pub-id><pub-id pub-id-type="pmid">25811410</pub-id></mixed-citation></ref><ref id="B5"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Althouse</surname><given-names>B. M.</given-names></name><name><surname>Hanley</surname><given-names>K. A.</given-names></name><name><surname>Diallo</surname><given-names>M.</given-names></name><name><surname>Sall</surname><given-names>A. A.</given-names></name><name><surname>Ba</surname><given-names>Y.</given-names></name><name><surname>Faye</surname><given-names>O.</given-names></name><etal></etal></person-group> (<year>2015</year>). <article-title>Impact of climate and mosquito vector abundance on sylvatic arbovirus circulation dynamics in Senegal.</article-title><source><italic>Am. J. Trop. Med. Hyg.</italic></source><volume>92</volume><fpage>88</fpage>–<lpage>97</lpage>. <pub-id pub-id-type="doi">10.4269/ajtmh.13-0617</pub-id><pub-id pub-id-type="pmid">25404071</pub-id></mixed-citation></ref><ref id="B6"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Apte-Sengupta</surname><given-names>S.</given-names></name><name><surname>Sirohi</surname><given-names>D.</given-names></name><name><surname>Kuhn</surname><given-names>R. J.</given-names></name></person-group> (<year>2014</year>). <article-title>Coupling of replication and assembly in flaviviruses.</article-title><source><italic>Curr. Opin. Virol.</italic></source><volume>9</volume><fpage>134</fpage>–<lpage>142</lpage>. <pub-id pub-id-type="doi">10.1016/j.coviro.2014.09.020S1879-6257(14)00202-8</pub-id><pub-id pub-id-type="pmid">25462445</pub-id></mixed-citation></ref><ref id="B7"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Aubry</surname><given-names>M.</given-names></name><name><surname>Richard</surname><given-names>V.</given-names></name><name><surname>Green</surname><given-names>J.</given-names></name><name><surname>Broult</surname><given-names>J.</given-names></name><name><surname>Musso</surname><given-names>D.</given-names></name></person-group> (<year>2016</year>). <article-title>Inactivation of Zika virus in plasma with amotosalen and ultraviolet A illumination.</article-title><source><italic>Transfusion</italic></source><volume>56</volume><fpage>33</fpage>–<lpage>40</lpage>. <pub-id pub-id-type="doi">10.1111/trf.13271</pub-id><pub-id pub-id-type="pmid">26283013</pub-id></mixed-citation></ref><ref id="B8"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Aubry</surname><given-names>M.</given-names></name><name><surname>Teissier</surname><given-names>A.</given-names></name><name><surname>Roche</surname><given-names>C.</given-names></name><name><surname>Richard</surname><given-names>V.</given-names></name><name><surname>Yan</surname><given-names>A. S.</given-names></name><name><surname>Zisou</surname><given-names>K.</given-names></name><etal></etal></person-group> (<year>2015</year>). <article-title>Chikungunya outbreak, French Polynesia, 2014.</article-title><source><italic>Emerg. Infect. Dis.</italic></source><volume>21</volume><fpage>724</fpage>–<lpage>726</lpage>. <pub-id pub-id-type="doi">10.3201/eid2104.141741</pub-id><pub-id pub-id-type="pmid">25811534</pub-id></mixed-citation></ref><ref id="B9"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Balm</surname><given-names>M. N.</given-names></name><name><surname>Lee</surname><given-names>C. K.</given-names></name><name><surname>Lee</surname><given-names>H. K.</given-names></name><name><surname>Chiu</surname><given-names>L.</given-names></name><name><surname>Koay</surname><given-names>E. S.</given-names></name><name><surname>Tang</surname><given-names>J. W.</given-names></name></person-group> (<year>2012</year>). <article-title>A diagnostic polymerase chain reaction assay for Zika virus.</article-title><source><italic>J. Med. Virol.</italic></source><volume>84</volume><fpage>1501</fpage>–<lpage>1505</lpage>. <pub-id pub-id-type="doi">10.1002/jmv.23241</pub-id><pub-id pub-id-type="pmid">22825831</pub-id></mixed-citation></ref><ref id="B10"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Baronti</surname><given-names>C.</given-names></name><name><surname>Piorkowski</surname><given-names>G.</given-names></name><name><surname>Charrel</surname><given-names>R. N.</given-names></name><name><surname>Boubis</surname><given-names>L.</given-names></name><name><surname>Leparc-Goffart</surname><given-names>I.</given-names></name><name><surname>de Lamballerie</surname><given-names>X.</given-names></name></person-group> (<year>2014</year>). <article-title>Complete coding sequence of zika virus from a French polynesia outbreak in 2013.</article-title><source><italic>Genome Announc</italic></source><volume>2</volume><issue>e500-14</issue><pub-id pub-id-type="doi">10.1128/genomeA.00500-14</pub-id></mixed-citation></ref><ref id="B11"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Bearcroft</surname><given-names>W. G.</given-names></name></person-group> (<year>1956</year>). <article-title>Zika virus infection experimentally induced in a human volunteer.</article-title><source><italic>Trans. R. Soc. Trop. Med. Hyg.</italic></source><volume>50</volume><fpage>442</fpage>–<lpage>448</lpage>. <pub-id pub-id-type="doi">10.1016/0035-9203(56)90090-6</pub-id><pub-id pub-id-type="pmid">13380987</pub-id></mixed-citation></ref><ref id="B12"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Bell</surname><given-names>T. M.</given-names></name><name><surname>Field</surname><given-names>E. J.</given-names></name><name><surname>Narang</surname><given-names>H. K.</given-names></name></person-group> (<year>1971</year>). <article-title>Zika virus infection of the central nervous system of mice.</article-title><source><italic>Arch. Gesamte Virusforsch</italic></source><volume>35</volume><fpage>183</fpage>–<lpage>193</lpage>. <pub-id pub-id-type="doi">10.1007/BF01249709</pub-id><pub-id pub-id-type="pmid">5002906</pub-id></mixed-citation></ref><ref id="B13"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ben-Nathan</surname><given-names>D.</given-names></name><name><surname>Lustig</surname><given-names>S.</given-names></name><name><surname>Tam</surname><given-names>G.</given-names></name><name><surname>Robinzon</surname><given-names>S.</given-names></name><name><surname>Segal</surname><given-names>S.</given-names></name><name><surname>Rager-Zisman</surname><given-names>B.</given-names></name></person-group> (<year>2003</year>). <article-title>Prophylactic and therapeutic efficacy of human intravenous immunoglobulin in treating West Nile virus infection in mice.</article-title><source><italic>J. Infect. Dis.</italic></source><volume>188</volume><fpage>5</fpage>–<lpage>12</lpage>. <pub-id pub-id-type="doi">10.1086/376870</pub-id><pub-id pub-id-type="pmid">12825165</pub-id></mixed-citation></ref><ref id="B14"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Berthet</surname><given-names>F. X.</given-names></name><name><surname>Zeller</surname><given-names>H. G.</given-names></name><name><surname>Drouet</surname><given-names>M. T.</given-names></name><name><surname>Rauzier</surname><given-names>J.</given-names></name><name><surname>Digoutte</surname><given-names>J. P.</given-names></name><name><surname>Deubel</surname><given-names>V.</given-names></name></person-group> (<year>1997</year>). <article-title>Extensive nucleotide changes and deletions within the envelope glycoprotein gene of Euro-African West Nile viruses.</article-title><source><italic>J. Gen. Virol.</italic></source><volume>78(Pt 9)</volume><fpage>2293</fpage>–<lpage>2297</lpage>. <pub-id pub-id-type="doi">10.1099/0022-1317-78-9-2293</pub-id><pub-id pub-id-type="pmid">9292017</pub-id></mixed-citation></ref><ref id="B15"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Berthet</surname><given-names>N.</given-names></name><name><surname>Nakoune</surname><given-names>E.</given-names></name><name><surname>Kamgang</surname><given-names>B.</given-names></name><name><surname>Selekon</surname><given-names>B.</given-names></name><name><surname>Descorps-Declere</surname><given-names>S.</given-names></name><name><surname>Gessain</surname><given-names>A.</given-names></name><etal></etal></person-group> (<year>2014</year>). <article-title>Molecular characterization of three Zika flaviviruses obtained from sylvatic mosquitoes in the Central African Republic.</article-title><source><italic>Vector Borne Zoonotic Dis.</italic></source><volume>14</volume><fpage>862</fpage>–<lpage>865</lpage>. <pub-id pub-id-type="doi">10.1089/vbz.2014.1607</pub-id><pub-id pub-id-type="pmid">25514122</pub-id></mixed-citation></ref><ref id="B16"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Besnard</surname><given-names>M.</given-names></name><name><surname>Lastere</surname><given-names>S.</given-names></name><name><surname>Teissier</surname><given-names>A.</given-names></name><name><surname>Cao-Lormeau</surname><given-names>V.</given-names></name><name><surname>Musso</surname><given-names>D.</given-names></name></person-group> (<year>2014</year>). <article-title>Evidence of perinatal transmission of Zika virus, French Polynesia, December 2013 and February 2014.</article-title><source><italic>Euro. Surveill.</italic></source><volume>19</volume>:<issue>20751</issue><pub-id pub-id-type="doi">10.2807/1560-7917.ES2014.19.13.20751</pub-id></mixed-citation></ref><ref id="B17"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Blazquez</surname><given-names>A. B.</given-names></name><name><surname>Escribano-Romero</surname><given-names>E.</given-names></name><name><surname>Martin-Acebes</surname><given-names>M. A.</given-names></name><name><surname>Petrovic</surname><given-names>T.</given-names></name><name><surname>Saiz</surname><given-names>J. C.</given-names></name></person-group> (<year>2015</year>). <article-title>Limited susceptibility of mice to Usutu virus (USUV) infection and induction of flavivirus cross-protective immunity.</article-title><source><italic>Virology</italic></source><volume>482</volume><fpage>67</fpage>–<lpage>71</lpage>. <pub-id pub-id-type="doi">10.1016/j.virol.2015.03.020</pub-id><pub-id pub-id-type="pmid">25827530</pub-id></mixed-citation></ref><ref id="B18"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Blazquez</surname><given-names>A. B.</given-names></name><name><surname>Escribano-Romero</surname><given-names>E.</given-names></name><name><surname>Merino-Ramos</surname><given-names>T.</given-names></name><name><surname>Saiz</surname><given-names>J. C.</given-names></name><name><surname>Martin-Acebes</surname><given-names>M. A.</given-names></name></person-group> (<year>2014</year>). <article-title>Stress responses in flavivirus-infected cells: activation of unfolded protein response and autophagy.</article-title><source><italic>Front. Microbiol.</italic></source><volume>5</volume>:<issue>266</issue><pub-id pub-id-type="doi">10.3389/fmicb.2014.00266</pub-id></mixed-citation></ref><ref id="B19"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Buathong</surname><given-names>R.</given-names></name><name><surname>Hermann</surname><given-names>L.</given-names></name><name><surname>Thaisomboonsuk</surname><given-names>B.</given-names></name><name><surname>Rutvisuttinunt</surname><given-names>W.</given-names></name><name><surname>Klungthong</surname><given-names>C.</given-names></name><name><surname>Chinnawirotpisan</surname><given-names>P.</given-names></name><etal></etal></person-group> (<year>2015</year>). <article-title>Detection of Zika Virus Infection in Thailand, 2012-2014.</article-title><source><italic>Am. J. Trop. Med. Hyg.</italic></source><volume>93</volume><fpage>380</fpage>–<lpage>383</lpage>. <pub-id pub-id-type="doi">10.4269/ajtmh.15-0022</pub-id><pub-id pub-id-type="pmid">26101272</pub-id></mixed-citation></ref><ref id="B20"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Buckley</surname><given-names>A.</given-names></name><name><surname>Gould</surname><given-names>E. A.</given-names></name></person-group> (<year>1988</year>). <article-title>Detection of virus-specific antigen in the nuclei or nucleoli of cells infected with Zika or Langat virus.</article-title><source><italic>J. Gen. Virol.</italic></source><volume>69(Pt 8)</volume><fpage>1913</fpage>–<lpage>1920</lpage>. <pub-id pub-id-type="doi">10.1099/0022-1317-69-8-1913</pub-id><pub-id pub-id-type="pmid">2841406</pub-id></mixed-citation></ref><ref id="B21"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Butler</surname><given-names>D.</given-names></name></person-group> (<year>2016</year>). <article-title>First Zika-linked birth defects detected in Colombia.</article-title><source><italic>Nature</italic></source><volume>531</volume>:<issue>153</issue><pub-id pub-id-type="doi">10.1038/nature.2016.19502</pub-id></mixed-citation></ref><ref id="B22"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Calvet</surname><given-names>G. A.</given-names></name><name><surname>Filippis</surname><given-names>A. M.</given-names></name><name><surname>Mendonca</surname><given-names>M. C.</given-names></name><name><surname>Sequeira</surname><given-names>P. C.</given-names></name><name><surname>Siqueira</surname><given-names>A. M.</given-names></name><name><surname>Veloso</surname><given-names>V. G.</given-names></name><etal></etal></person-group> (<year>2016</year>). <article-title>First detection of autochthonous Zika virus transmission in a HIV-infected patient in Rio de Janeiro.</article-title><source><italic>Brazil. J. Clin. Virol.</italic></source><volume>74</volume><fpage>1</fpage>–<lpage>3</lpage>. <pub-id pub-id-type="doi">10.1016/j.jcv.2015.11.014</pub-id><pub-id pub-id-type="pmid">26615388</pub-id></mixed-citation></ref><ref id="B23"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Campos</surname><given-names>G. S.</given-names></name><name><surname>Bandeira</surname><given-names>A. C.</given-names></name><name><surname>Sardi</surname><given-names>S. I.</given-names></name></person-group> (<year>2015</year>). <article-title>Zika Virus outbreak, Bahia, Brazil.</article-title><source><italic>Emerg. Infect. Dis.</italic></source><volume>21</volume><fpage>1885</fpage>–<lpage>1886</lpage>. <pub-id pub-id-type="doi">10.3201/eid2110.150847</pub-id><pub-id pub-id-type="pmid">26401719</pub-id></mixed-citation></ref><ref id="B24"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Cao-Lormeau</surname><given-names>V. M.</given-names></name><name><surname>Roche</surname><given-names>C.</given-names></name><name><surname>Teissier</surname><given-names>A.</given-names></name><name><surname>Robin</surname><given-names>E.</given-names></name><name><surname>Berry</surname><given-names>A. L.</given-names></name><name><surname>Mallet</surname><given-names>H. P.</given-names></name><etal></etal></person-group> (<year>2014</year>). <article-title>Zika virus, French polynesia, South pacific, 2013.</article-title><source><italic>Emerg. Infect. Dis.</italic></source><volume>20</volume><fpage>1085</fpage>–<lpage>1086</lpage>. <pub-id pub-id-type="doi">10.3201/eid2006.140138</pub-id><pub-id pub-id-type="pmid">24856001</pub-id></mixed-citation></ref><ref id="B25"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Caron</surname><given-names>M.</given-names></name><name><surname>Paupy</surname><given-names>C.</given-names></name><name><surname>Grard</surname><given-names>G.</given-names></name><name><surname>Becquart</surname><given-names>P.</given-names></name><name><surname>Mombo</surname><given-names>I.</given-names></name><name><surname>Nso</surname><given-names>B. B.</given-names></name><etal></etal></person-group> (<year>2012</year>). <article-title>Recent introduction and rapid dissemination of Chikungunya virus and Dengue virus serotype 2 associated with human and mosquito coinfections in Gabon, central Africa.</article-title><source><italic>Clin. Infect. Dis.</italic></source><volume>55</volume><fpage>e45</fpage>–<lpage>e53</lpage>. <pub-id pub-id-type="doi">10.1093/cid/cis530cis530</pub-id><pub-id pub-id-type="pmid">22670036</pub-id></mixed-citation></ref><ref id="B26"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Daffis</surname><given-names>S.</given-names></name><name><surname>Szretter</surname><given-names>K. J.</given-names></name><name><surname>Schriewer</surname><given-names>J.</given-names></name><name><surname>Li</surname><given-names>J.</given-names></name><name><surname>Youn</surname><given-names>S.</given-names></name><name><surname>Errett</surname><given-names>J.</given-names></name><etal></etal></person-group> (<year>2010</year>). <article-title>2’-O methylation of the viral mRNA cap evades host restriction by IFIT family members.</article-title><source><italic>Nature</italic></source><volume>468</volume><fpage>452</fpage>–<lpage>456</lpage>. <pub-id pub-id-type="doi">10.1038/nature09489</pub-id><pub-id pub-id-type="pmid">21085181</pub-id></mixed-citation></ref><ref id="B27"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Darwish</surname><given-names>M. A.</given-names></name><name><surname>Hoogstraal</surname><given-names>H.</given-names></name><name><surname>Roberts</surname><given-names>T. J.</given-names></name><name><surname>Ahmed</surname><given-names>I. P.</given-names></name><name><surname>Omar</surname><given-names>F.</given-names></name></person-group> (<year>1983</year>). <article-title>A sero-epidemiological survey for certain arboviruses (Togaviridae) in Pakistan.</article-title><source><italic>Trans. R. Soc. Trop. Med. Hyg.</italic></source><volume>77</volume><fpage>442</fpage>–<lpage>445</lpage>. <pub-id pub-id-type="doi">10.1016/0035-9203(83)90108-6</pub-id><pub-id pub-id-type="pmid">6314612</pub-id></mixed-citation></ref><ref id="B28"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Dauphin</surname><given-names>G.</given-names></name><name><surname>Zientara</surname><given-names>S.</given-names></name></person-group> (<year>2007</year>). <article-title>West Nile virus: recent trends in diagnosis and vaccine development.</article-title><source><italic>Vaccine</italic></source><volume>25</volume><fpage>5563</fpage>–<lpage>5576</lpage>. <pub-id pub-id-type="doi">10.1016/j.vaccine.2006.12.005</pub-id><pub-id pub-id-type="pmid">17292514</pub-id></mixed-citation></ref><ref id="B29"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>de Paula Freitas</surname><given-names>B.</given-names></name><name><surname>de Oliveira Dias</surname><given-names>J. R.</given-names></name><name><surname>Prazeres</surname><given-names>J.</given-names></name><name><surname>Sacramento</surname><given-names>G. A.</given-names></name><name><surname>Ko</surname><given-names>A. I.</given-names></name><name><surname>Maia</surname><given-names>M.</given-names></name><etal></etal></person-group> (<year>2016</year>). <article-title>Ocular findings in infants with microcephaly associated with presumed zika virus congenital infection in salvador, brazil.</article-title><source><italic>JAMA Ophthalmol.</italic></source><pub-id pub-id-type="doi">10.1001/jamaophthalmol.2016.0267</pub-id><comment>[Epub ahead of print].</comment></mixed-citation></ref><ref id="B30"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Delatte</surname><given-names>H.</given-names></name><name><surname>Desvars</surname><given-names>A.</given-names></name><name><surname>Bouetard</surname><given-names>A.</given-names></name><name><surname>Bord</surname><given-names>S.</given-names></name><name><surname>Gimonneau</surname><given-names>G.</given-names></name><name><surname>Vourc’h</surname><given-names>G.</given-names></name><etal></etal></person-group> (<year>2010</year>). <article-title>Blood-feeding behavior of <italic>Aedes albopictus</italic>, a vector of chikungunya on la reunion.</article-title><source><italic>Vector Borne Zoonotic Dis.</italic></source><volume>10</volume><fpage>249</fpage>–<lpage>258</lpage>. <pub-id pub-id-type="doi">10.1089/vbz.2009.0026</pub-id><pub-id pub-id-type="pmid">19589060</pub-id></mixed-citation></ref><ref id="B31"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Delisle</surname><given-names>E.</given-names></name><name><surname>Rousseau</surname><given-names>C.</given-names></name><name><surname>Broche</surname><given-names>B.</given-names></name><name><surname>Leparc-Goffart</surname><given-names>I.</given-names></name><name><surname>L’Ambert</surname><given-names>G.</given-names></name><name><surname>Cochet</surname><given-names>A.</given-names></name><etal></etal></person-group> (<year>2015</year>). <article-title>Chikungunya outbreak in Montpellier, France, September to October 2014.</article-title><source><italic>Euro Surveill.</italic></source><volume>20</volume>:<issue>21108</issue><pub-id pub-id-type="doi">10.2807/1560-7917.ES2015.20.17.21108</pub-id></mixed-citation></ref><ref id="B32"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Dereeper</surname><given-names>A.</given-names></name><name><surname>Guignon</surname><given-names>V.</given-names></name><name><surname>Blanc</surname><given-names>G.</given-names></name><name><surname>Audic</surname><given-names>S.</given-names></name><name><surname>Buffet</surname><given-names>S.</given-names></name><name><surname>Chevenet</surname><given-names>F.</given-names></name><etal></etal></person-group> (<year>2008</year>). <article-title>Phylogeny.fr: robust phylogenetic analysis for the non-specialist.</article-title><source><italic>Nucleic Acids Res.</italic></source><volume>36</volume><fpage>W465</fpage>–<lpage>W469</lpage>. <pub-id pub-id-type="doi">10.1093/nar/gkn180</pub-id><pub-id pub-id-type="pmid">18424797</pub-id></mixed-citation></ref><ref id="B33"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Diagne</surname><given-names>C. T.</given-names></name><name><surname>Diallo</surname><given-names>D.</given-names></name><name><surname>Faye</surname><given-names>O.</given-names></name><name><surname>Ba</surname><given-names>Y.</given-names></name><name><surname>Gaye</surname><given-names>A.</given-names></name><name><surname>Dia</surname><given-names>I.</given-names></name><etal></etal></person-group> (<year>2015</year>). <article-title>Potential of selected senegalese <italic>Aedes</italic> spp. mosquitoes (Diptera: Culicidae) to transmit Zika virus.</article-title><source><italic>BMC Infect. Dis.</italic></source><volume>15</volume>:<issue>492</issue><pub-id pub-id-type="doi">10.1186/s12879-015-1231-2</pub-id></mixed-citation></ref><ref id="B34"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Diallo</surname><given-names>D.</given-names></name><name><surname>Sall</surname><given-names>A. A.</given-names></name><name><surname>Diagne</surname><given-names>C. T.</given-names></name><name><surname>Faye</surname><given-names>O.</given-names></name><name><surname>Ba</surname><given-names>Y.</given-names></name><name><surname>Hanley</surname><given-names>K. A.</given-names></name><etal></etal></person-group> (<year>2014</year>). <article-title>Zika virus emergence in mosquitoes in southeastern Senegal, 2011.</article-title><source><italic>PLoS ONE</italic></source><volume>9</volume>:<issue>e109442</issue><pub-id pub-id-type="doi">10.1371/journal.pone.0109442</pub-id></mixed-citation></ref><ref id="B35"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Dick</surname><given-names>G. W.</given-names></name></person-group> (<year>1952</year>). <article-title>Zika virus. II. Pathogenicity and physical properties.</article-title><source><italic>Trans. R. Soc. Trop. Med. Hyg.</italic></source><volume>46</volume><fpage>521</fpage>–<lpage>534</lpage>. <pub-id pub-id-type="doi">10.1016/0035-9203(52)90043-6</pub-id><pub-id pub-id-type="pmid">12995441</pub-id></mixed-citation></ref><ref id="B36"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Dick</surname><given-names>G. W.</given-names></name><name><surname>Kitchen</surname><given-names>S. F.</given-names></name><name><surname>Haddow</surname><given-names>A. J.</given-names></name></person-group> (<year>1952</year>). <article-title>Zika virus. I. Isolations and serological specificity.</article-title><source><italic>Trans. R. Soc. Trop. Med. Hyg.</italic></source><volume>46</volume><fpage>509</fpage>–<lpage>520</lpage>. <pub-id pub-id-type="doi">10.1016/0035-9203(52)90042-4</pub-id><pub-id pub-id-type="pmid">12995440</pub-id></mixed-citation></ref><ref id="B37"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Dominguez-Moreno</surname><given-names>R.</given-names></name><name><surname>Tolosa-Tort</surname><given-names>P.</given-names></name><name><surname>Patino-Tamez</surname><given-names>A.</given-names></name><name><surname>Quintero-Bauman</surname><given-names>A.</given-names></name><name><surname>Collado-Frias</surname><given-names>D. K.</given-names></name><name><surname>Miranda-Rodriguez</surname><given-names>M. G.</given-names></name><etal></etal></person-group> (<year>2014</year>). <article-title>[Mortality associated with a diagnosis of Guillain-Barre syndrome in adults of Mexican health institutions].</article-title><source><italic>Rev. Neurol.</italic></source><volume>58</volume><fpage>4</fpage>–<lpage>10</lpage>.<pub-id pub-id-type="pmid">24343535</pub-id></mixed-citation></ref><ref id="B38"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Dong</surname><given-names>H.</given-names></name><name><surname>Fink</surname><given-names>K.</given-names></name><name><surname>Zust</surname><given-names>R.</given-names></name><name><surname>Lim</surname><given-names>S. P.</given-names></name><name><surname>Qin</surname><given-names>C. F.</given-names></name><name><surname>Shi</surname><given-names>P. Y.</given-names></name></person-group> (<year>2014</year>). <article-title>Flavivirus RNA methylation.</article-title><source><italic>J. Gen. Virol.</italic></source><volume>95</volume><fpage>763</fpage>–<lpage>778</lpage>. <pub-id pub-id-type="doi">10.1099/vir.0.062208-0</pub-id><pub-id pub-id-type="pmid">24486628</pub-id></mixed-citation></ref><ref id="B39"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Duffy</surname><given-names>M. R.</given-names></name><name><surname>Chen</surname><given-names>T. H.</given-names></name><name><surname>Hancock</surname><given-names>W. T.</given-names></name><name><surname>Powers</surname><given-names>A. M.</given-names></name><name><surname>Kool</surname><given-names>J. L.</given-names></name><name><surname>Lanciotti</surname><given-names>R. S.</given-names></name><etal></etal></person-group> (<year>2009</year>). <article-title>Zika virus outbreak on Yap Island, Federated States of Micronesia.</article-title><source><italic>N. Engl. J. Med.</italic></source><volume>360</volume><fpage>2536</fpage>–<lpage>2543</lpage>. <pub-id pub-id-type="doi">10.1056/NEJMoa0805715</pub-id><pub-id pub-id-type="pmid">19516034</pub-id></mixed-citation></ref><ref id="B40"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Dupont-Rouzeyrol</surname><given-names>M.</given-names></name><name><surname>O’Connor</surname><given-names>O.</given-names></name><name><surname>Calvez</surname><given-names>E.</given-names></name><name><surname>Daures</surname><given-names>M.</given-names></name><name><surname>John</surname><given-names>M.</given-names></name><name><surname>Grangeon</surname><given-names>J. P.</given-names></name><etal></etal></person-group> (<year>2015</year>). <article-title>Co-infection with Zika and dengue viruses in 2 patients, New Caledonia, 2014.</article-title><source><italic>Emerg. Infect. Dis.</italic></source><volume>21</volume><fpage>381</fpage>–<lpage>382</lpage>. <pub-id pub-id-type="doi">10.3201/eid2102.141553</pub-id><pub-id pub-id-type="pmid">25625687</pub-id></mixed-citation></ref><ref id="B41"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Dyer</surname><given-names>O.</given-names></name></person-group> (<year>2016</year>). <article-title>Sixty seconds on, Zika virus.</article-title><source><italic>BMJ</italic></source><volume>352</volume>:<issue>467</issue><pub-id pub-id-type="doi">10.1136/bmj.i467</pub-id></mixed-citation></ref><ref id="B42"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Engle</surname><given-names>M. J.</given-names></name><name><surname>Diamond</surname><given-names>M. S.</given-names></name></person-group> (<year>2003</year>). <article-title>Antibody prophylaxis and therapy against West Nile virus infection in wild-type and immunodeficient mice.</article-title><source><italic>J. Virol.</italic></source><volume>77</volume><fpage>12941</fpage>–<lpage>12949</lpage>. <pub-id pub-id-type="doi">10.1128/JVI.77.24.12941-12949.2003</pub-id><pub-id pub-id-type="pmid">14645550</pub-id></mixed-citation></ref><ref id="B43"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Fagbami</surname><given-names>A. H.</given-names></name></person-group> (<year>1979</year>). <article-title>Zika virus infections in Nigeria: virological and seroepidemiological investigations in Oyo State.</article-title><source><italic>J. Hyg.</italic></source><volume>83</volume><fpage>213</fpage>–<lpage>219</lpage>. <pub-id pub-id-type="doi">10.1017/S0022172400025997</pub-id><pub-id pub-id-type="pmid">489960</pub-id></mixed-citation></ref><ref id="B44"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Fauci</surname><given-names>A. S.</given-names></name><name><surname>Morens</surname><given-names>D. M.</given-names></name></person-group> (<year>2016</year>). <article-title>Zika virus in the americas–yet another arbovirus threat.</article-title><source><italic>N. Engl. J. Med.</italic></source><volume>374</volume><fpage>601</fpage>–<lpage>604</lpage>. <pub-id pub-id-type="doi">10.1056/NEJMp1600297</pub-id><pub-id pub-id-type="pmid">26761185</pub-id></mixed-citation></ref><ref id="B45"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Faye</surname><given-names>O.</given-names></name><name><surname>Diallo</surname><given-names>D.</given-names></name><name><surname>Diallo</surname><given-names>M.</given-names></name><name><surname>Weidmann</surname><given-names>M.</given-names></name><name><surname>Sall</surname><given-names>A. A.</given-names></name></person-group> (<year>2013</year>). <article-title>Quantitative real-time PCR detection of Zika virus and evaluation with field-caught mosquitoes.</article-title><source><italic>Virol. J.</italic></source><volume>10</volume>:<issue>311</issue><pub-id pub-id-type="doi">10.1186/1743-422X-10-311</pub-id></mixed-citation></ref><ref id="B46"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Faye</surname><given-names>O.</given-names></name><name><surname>Dupressoir</surname><given-names>A.</given-names></name><name><surname>Weidmann</surname><given-names>M.</given-names></name><name><surname>Ndiaye</surname><given-names>M.</given-names></name><name><surname>Alpha Sall</surname><given-names>A.</given-names></name></person-group> (<year>2008</year>). <article-title>One-step RT-PCR for detection of Zika virus.</article-title><source><italic>J. Clin. Virol.</italic></source><volume>43</volume><fpage>96</fpage>–<lpage>101</lpage>. <pub-id pub-id-type="doi">10.1016/j.jcv.2008.05.005</pub-id><pub-id pub-id-type="pmid">18674965</pub-id></mixed-citation></ref><ref id="B47"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Faye</surname><given-names>O.</given-names></name><name><surname>Freire</surname><given-names>C. C.</given-names></name><name><surname>Iamarino</surname><given-names>A.</given-names></name><name><surname>de Oliveira</surname><given-names>J. V.</given-names></name><name><surname>Diallo</surname><given-names>M.</given-names></name><name><surname>Zanotto</surname><given-names>P. M.</given-names></name><etal></etal></person-group> (<year>2014</year>). <article-title>Molecular evolution of Zika virus during its emergence in the 20(th) century.</article-title><source><italic>PLoS Negl. Trop. Dis.</italic></source><volume>8</volume>:<issue>e2636</issue><pub-id pub-id-type="doi">10.1371/journal.pntd.0002636</pub-id></mixed-citation></ref><ref id="B48"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Filipe</surname><given-names>A. R.</given-names></name><name><surname>Martins</surname><given-names>C. M.</given-names></name><name><surname>Rocha</surname><given-names>H.</given-names></name></person-group> (<year>1973</year>). <article-title>Laboratory infection with Zika virus after vaccination against yellow fever.</article-title><source><italic>Arch. Gesamte Virusforsch</italic></source><volume>43</volume><fpage>315</fpage>–<lpage>319</lpage>. <pub-id pub-id-type="doi">10.1007/BF01556147</pub-id><pub-id pub-id-type="pmid">4799154</pub-id></mixed-citation></ref><ref id="B49"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Foy</surname><given-names>B. D.</given-names></name><name><surname>Kobylinski</surname><given-names>K. C.</given-names></name><name><surname>Chilson Foy</surname><given-names>J. L.</given-names></name><name><surname>Blitvich</surname><given-names>B. J.</given-names></name><name><surname>Travassos da Rosa</surname><given-names>A.</given-names></name><name><surname>Haddow</surname><given-names>A. D.</given-names></name><etal></etal></person-group> (<year>2011</year>). <article-title>Probable non-vector-borne transmission of Zika virus, Colorado, USA.</article-title><source><italic>Emerg. Infect. Dis.</italic></source><volume>17</volume><fpage>880</fpage>–<lpage>882</lpage>. <pub-id pub-id-type="doi">10.3201/eid1705.101939</pub-id><pub-id pub-id-type="pmid">21529401</pub-id></mixed-citation></ref><ref id="B50"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Freire</surname><given-names>C. C. D. M.</given-names></name><name><surname>Iamarino</surname><given-names>A.</given-names></name><name><surname>Neto</surname><given-names>D. F. D. L.</given-names></name><name><surname>Sall</surname><given-names>A. A.</given-names></name><name><surname>Zanotto</surname><given-names>P. M. D. A.</given-names></name></person-group> (<year>2015</year>). <article-title>Spread of the pandemic Zika virus lineage is associated with NS1 codon usage adaptation in humans.</article-title><source><italic>bioRxiv</italic></source><pub-id pub-id-type="doi">10.1101/032839</pub-id></mixed-citation></ref><ref id="B51"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gatherer</surname><given-names>D.</given-names></name><name><surname>Kohl</surname><given-names>A.</given-names></name></person-group> (<year>2016</year>). <article-title>Zika virus: a previously slow pandemic spreads rapidly through the Americas.</article-title><source><italic>J. Gen. Virol.</italic></source><volume>97</volume><fpage>269</fpage>–<lpage>273</lpage>. <pub-id pub-id-type="doi">10.1099/jgv.0.000381</pub-id><pub-id pub-id-type="pmid">26684466</pub-id></mixed-citation></ref><ref id="B52"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Geser</surname><given-names>A.</given-names></name><name><surname>Henderson</surname><given-names>B. E.</given-names></name><name><surname>Christensen</surname><given-names>S.</given-names></name></person-group> (<year>1970</year>). <article-title>A multipurpose serological survey in Kenya. 2. Results of arbovirus serological tests.</article-title><source><italic>Bull. World Health Organ.</italic></source><volume>43</volume><fpage>539</fpage>–<lpage>552</lpage>.<pub-id pub-id-type="pmid">5313066</pub-id></mixed-citation></ref><ref id="B53"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gjenero-Margan</surname><given-names>I.</given-names></name><name><surname>Aleraj</surname><given-names>B.</given-names></name><name><surname>Krajcar</surname><given-names>D.</given-names></name><name><surname>Lesnikar</surname><given-names>V.</given-names></name><name><surname>Klobucar</surname><given-names>A.</given-names></name><name><surname>Pem-Novosel</surname><given-names>I.</given-names></name><etal></etal></person-group> (<year>2011</year>). <article-title>Autochthonous dengue fever in Croatia, August-September 2010.</article-title><source><italic>Euro Surveill.</italic></source><volume>16</volume>:<issue>19805</issue>.</mixed-citation></ref><ref id="B54"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gollins</surname><given-names>S. W.</given-names></name><name><surname>Porterfield</surname><given-names>J. S.</given-names></name></person-group> (<year>1986</year>). <article-title>The uncoating and infectivity of the flavivirus West Nile on interaction with cells: effects of pH and ammonium chloride.</article-title><source><italic>J. Gen. Virol.</italic></source><volume>67(Pt 9)</volume><fpage>1941</fpage>–<lpage>1950</lpage>. <pub-id pub-id-type="doi">10.1099/0022-1317-67-9-1941</pub-id><pub-id pub-id-type="pmid">3746254</pub-id></mixed-citation></ref><ref id="B55"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gould</surname><given-names>E. A.</given-names></name><name><surname>Solomon</surname><given-names>T.</given-names></name></person-group> (<year>2008</year>). <article-title>Pathogenic flaviviruses.</article-title><source><italic>Lancet</italic></source><volume>371</volume><fpage>500</fpage>–<lpage>509</lpage>. <pub-id pub-id-type="doi">10.1016/S0140-6736(08)60238-X</pub-id><pub-id pub-id-type="pmid">18262042</pub-id></mixed-citation></ref><ref id="B56"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gourinat</surname><given-names>A. C.</given-names></name><name><surname>O’Connor</surname><given-names>O.</given-names></name><name><surname>Calvez</surname><given-names>E.</given-names></name><name><surname>Goarant</surname><given-names>C.</given-names></name><name><surname>Dupont-Rouzeyrol</surname><given-names>M.</given-names></name></person-group> (<year>2015</year>). <article-title>Detection of Zika virus in urine.</article-title><source><italic>Emerg. Infect. Dis.</italic></source><volume>21</volume><fpage>84</fpage>–<lpage>86</lpage>. <pub-id pub-id-type="doi">10.3201/eid2101.140894</pub-id><pub-id pub-id-type="pmid">25530324</pub-id></mixed-citation></ref><ref id="B57"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Grard</surname><given-names>G.</given-names></name><name><surname>Caron</surname><given-names>M.</given-names></name><name><surname>Mombo</surname><given-names>I. M.</given-names></name><name><surname>Nkoghe</surname><given-names>D.</given-names></name><name><surname>Mboui Ondo</surname><given-names>S.</given-names></name><name><surname>Jiolle</surname><given-names>D.</given-names></name><etal></etal></person-group> (<year>2014</year>). <article-title>Zika virus in Gabon (Central Africa)–2007: a new threat from <italic>Aedes albopictus</italic>?</article-title><source><italic>PLoS Negl. Trop. Dis.</italic></source><volume>8</volume>:<issue>e2681</issue><pub-id pub-id-type="doi">10.1371/journal.pntd.0002681</pub-id></mixed-citation></ref><ref id="B58"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Haddow</surname><given-names>A. D.</given-names></name><name><surname>Schuh</surname><given-names>A. J.</given-names></name><name><surname>Yasuda</surname><given-names>C. Y.</given-names></name><name><surname>Kasper</surname><given-names>M. R.</given-names></name><name><surname>Heang</surname><given-names>V.</given-names></name><name><surname>Huy</surname><given-names>R.</given-names></name><etal></etal></person-group> (<year>2012</year>). <article-title>Genetic characterization of Zika virus strains: geographic expansion of the Asian lineage.</article-title><source><italic>PLoS Negl. Trop. Dis.</italic></source><volume>6</volume>:<issue>e1477</issue><pub-id pub-id-type="doi">10.1371/journal.pntd.0001477</pub-id></mixed-citation></ref><ref id="B59"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Haddow</surname><given-names>A. J.</given-names></name><name><surname>Dick</surname><given-names>G. W.</given-names></name></person-group> (<year>1948</year>). <article-title>Catches of biting Diptera in Uganda, with anaesthetized monkeys as bait.</article-title><source><italic>Ann. Trop. Med. Parasitol.</italic></source><volume>42</volume><fpage>271</fpage>–<lpage>277</lpage>. <pub-id pub-id-type="doi">10.1080/00034983.1948.11685376</pub-id><pub-id pub-id-type="pmid">18110343</pub-id></mixed-citation></ref><ref id="B60"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Haddow</surname><given-names>A. J.</given-names></name><name><surname>Williams</surname><given-names>M. C.</given-names></name><name><surname>Woodall</surname><given-names>J. P.</given-names></name><name><surname>Simpson</surname><given-names>D. I.</given-names></name><name><surname>Goma</surname><given-names>L. K.</given-names></name></person-group> (<year>1964</year>). <article-title>Twelve isolations of Zika virus from <italic>Aedes</italic> (<italic>Stegomyia</italic>) Africanus (Theobald) Taken in and above a Uganda Forest.</article-title><source><italic>Bull. World Health Organ.</italic></source><volume>31</volume><fpage>57</fpage>–<lpage>69</lpage>.<pub-id pub-id-type="pmid">14230895</pub-id></mixed-citation></ref><ref id="B61"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hamel</surname><given-names>R.</given-names></name><name><surname>Dejarnac</surname><given-names>O.</given-names></name><name><surname>Wichit</surname><given-names>S.</given-names></name><name><surname>Ekchariyawat</surname><given-names>P.</given-names></name><name><surname>Neyret</surname><given-names>A.</given-names></name><name><surname>Luplertlop</surname><given-names>N.</given-names></name><etal></etal></person-group> (<year>2015</year>). <article-title>Biology of Zika virus infection in human skin cells.</article-title><source><italic>J. Virol.</italic></source><volume>89</volume><fpage>8880</fpage>–<lpage>8896</lpage>. <pub-id pub-id-type="doi">10.1128/JVI.00354-15</pub-id><pub-id pub-id-type="pmid">26085147</pub-id></mixed-citation></ref><ref id="B62"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hammon</surname><given-names>W. M.</given-names></name><name><surname>Schrack</surname><given-names>W. D.</given-names><suffix>Jr.</suffix></name><name><surname>Sather</surname><given-names>G. E.</given-names></name></person-group> (<year>1958</year>). <article-title>Serological survey for a arthropod-borne virus infections in the Philippines.</article-title><source><italic>Am. J. Trop. Med. Hyg.</italic></source><volume>7</volume><fpage>323</fpage>–<lpage>328</lpage>.<pub-id pub-id-type="pmid">13533740</pub-id></mixed-citation></ref><ref id="B63"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Heang</surname><given-names>V.</given-names></name><name><surname>Yasuda</surname><given-names>C. Y.</given-names></name><name><surname>Sovann</surname><given-names>L.</given-names></name><name><surname>Haddow</surname><given-names>A. D.</given-names></name><name><surname>Travassos da Rosa</surname><given-names>A. P.</given-names></name><name><surname>Tesh</surname><given-names>R. B.</given-names></name><etal></etal></person-group> (<year>2012</year>). <article-title>Zika virus infection, Cambodia, 2010.</article-title><source><italic>Emerg. Infect. Dis.</italic></source><volume>18</volume><fpage>349</fpage>–<lpage>351</lpage>. <pub-id pub-id-type="doi">10.3201/eid1802.111224</pub-id><pub-id pub-id-type="pmid">22305269</pub-id></mixed-citation></ref><ref id="B64"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Higgs</surname><given-names>S.</given-names></name></person-group> (<year>2016</year>). <article-title>Zika virus: emergence and emergency.</article-title><source><italic>Vector Borne Zoonotic Dis.</italic></source><volume>16</volume><fpage>75</fpage>–<lpage>76</lpage>. <pub-id pub-id-type="doi">10.1089/vbz.2016.29001.hig</pub-id><pub-id pub-id-type="pmid">26824625</pub-id></mixed-citation></ref><ref id="B65"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Holmes</surname><given-names>E. C.</given-names></name></person-group> (<year>2003</year>). <article-title>Patterns of intra- and interhost nonsynonymous variation reveal strong purifying selection in dengue virus.</article-title><source><italic>J. Virol.</italic></source><volume>77</volume><fpage>11296</fpage>–<lpage>11298</lpage>. <pub-id pub-id-type="doi">10.1128/JVI.77.20.11296-11298.2003</pub-id><pub-id pub-id-type="pmid">14512579</pub-id></mixed-citation></ref><ref id="B66"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ishikawa</surname><given-names>T.</given-names></name><name><surname>Yamanaka</surname><given-names>A.</given-names></name><name><surname>Konishi</surname><given-names>E.</given-names></name></person-group> (<year>2014</year>). <article-title>A review of successful flavivirus vaccines and the problems with those flaviviruses for which vaccines are not yet available.</article-title><source><italic>Vaccine</italic></source><volume>32</volume><fpage>1326</fpage>–<lpage>1337</lpage>. <pub-id pub-id-type="doi">10.1016/j.vaccine.2014.01.040S0264-410X(14)00074-7</pub-id><pub-id pub-id-type="pmid">24486372</pub-id></mixed-citation></ref><ref id="B67"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jan</surname><given-names>C.</given-names></name><name><surname>Languillat</surname><given-names>G.</given-names></name><name><surname>Renaudet</surname><given-names>J.</given-names></name><name><surname>Robin</surname><given-names>Y.</given-names></name></person-group> (<year>1978</year>). <article-title>[A serological survey of arboviruses in Gabon].</article-title><source><italic>Bull. Soc. Pathol. Exot. Filiales</italic></source><volume>71</volume><fpage>140</fpage>–<lpage>146</lpage>.<pub-id pub-id-type="pmid">743766</pub-id></mixed-citation></ref><ref id="B68"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Johnson</surname><given-names>B. K.</given-names></name><name><surname>Chanas</surname><given-names>A. C.</given-names></name><name><surname>Shockley</surname><given-names>P.</given-names></name><name><surname>Squires</surname><given-names>E. J.</given-names></name><name><surname>Gardner</surname><given-names>P.</given-names></name><name><surname>Wallace</surname><given-names>C.</given-names></name><etal></etal></person-group> (<year>1977</year>). <article-title>Arbovirus isolations from, and serological studies on, wild and domestic vertebrates from Kano Plain, Kenya.</article-title><source><italic>Trans. R. Soc. Trop. Med. Hyg.</italic></source><volume>71</volume><fpage>512</fpage>–<lpage>517</lpage>. <pub-id pub-id-type="doi">10.1016/0035-9203(77)90146-8</pub-id><pub-id pub-id-type="pmid">605465</pub-id></mixed-citation></ref><ref id="B69"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kraemer</surname><given-names>M. U.</given-names></name><name><surname>Sinka</surname><given-names>M. E.</given-names></name><name><surname>Duda</surname><given-names>K. A.</given-names></name><name><surname>Mylne</surname><given-names>A. Q.</given-names></name><name><surname>Shearer</surname><given-names>F. M.</given-names></name><name><surname>Barker</surname><given-names>C. M.</given-names></name><etal></etal></person-group> (<year>2015</year>). <article-title>The global distribution of the arbovirus vectors <italic>Aedes aegypti</italic> and <italic>A. albopictus</italic>.</article-title><source><italic>eLife</italic></source><volume>4</volume>:<issue>e08347</issue><pub-id pub-id-type="doi">10.7554/eLife.08347</pub-id></mixed-citation></ref><ref id="B70"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kuhn</surname><given-names>R. J.</given-names></name><name><surname>Zhang</surname><given-names>W.</given-names></name><name><surname>Rossmann</surname><given-names>M. G.</given-names></name><name><surname>Pletnev</surname><given-names>S. V.</given-names></name><name><surname>Corver</surname><given-names>J.</given-names></name><name><surname>Lenches</surname><given-names>E.</given-names></name><etal></etal></person-group> (<year>2002</year>). <article-title>Structure of dengue virus: implications for flavivirus organization, maturation, and fusion.</article-title><source><italic>Cell</italic></source><volume>108</volume><fpage>717</fpage>–<lpage>725</lpage>. <pub-id pub-id-type="doi">10.1016/S0092-8674(02)00660-8</pub-id><pub-id pub-id-type="pmid">11893341</pub-id></mixed-citation></ref><ref id="B71"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kuno</surname><given-names>G.</given-names></name><name><surname>Chang</surname><given-names>G. J.</given-names></name></person-group> (<year>2007</year>). <article-title>Full-length sequencing and genomic characterization of Bagaza, Kedougou, and Zika viruses.</article-title><source><italic>Arch. Virol.</italic></source><volume>152</volume><fpage>687</fpage>–<lpage>696</lpage>. <pub-id pub-id-type="doi">10.1007/s00705-006-0903-z</pub-id><pub-id pub-id-type="pmid">17195954</pub-id></mixed-citation></ref><ref id="B72"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kuno</surname><given-names>G.</given-names></name><name><surname>Chang</surname><given-names>G. J.</given-names></name><name><surname>Tsuchiya</surname><given-names>K. R.</given-names></name><name><surname>Karabatsos</surname><given-names>N.</given-names></name><name><surname>Cropp</surname><given-names>C. B.</given-names></name></person-group> (<year>1998</year>). <article-title>Phylogeny of the genus Flavivirus.</article-title><source><italic>J. Virol.</italic></source><volume>72</volume><fpage>73</fpage>–<lpage>83</lpage>.<pub-id pub-id-type="pmid">9420202</pub-id></mixed-citation></ref><ref id="B73"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kutsuna</surname><given-names>S.</given-names></name><name><surname>Kato</surname><given-names>Y.</given-names></name><name><surname>Takasaki</surname><given-names>T.</given-names></name><name><surname>Moi</surname><given-names>M.</given-names></name><name><surname>Kotaki</surname><given-names>A.</given-names></name><name><surname>Uemura</surname><given-names>H.</given-names></name><etal></etal></person-group> (<year>2014</year>). <article-title>Two cases of Zika fever imported from French Polynesia to Japan, December 2013 to January 2014 [corrected].</article-title><source><italic>Euro Surveill.</italic></source><volume>19</volume>:<issue>20683</issue><pub-id pub-id-type="doi">10.2807/1560-7917.ES2014.19.4.20683</pub-id></mixed-citation></ref><ref id="B74"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kwong</surname><given-names>J. C.</given-names></name><name><surname>Druce</surname><given-names>J. D.</given-names></name><name><surname>Leder</surname><given-names>K.</given-names></name></person-group> (<year>2013</year>). <article-title>Zika virus infection acquired during brief travel to Indonesia.</article-title><source><italic>Am. J. Trop. Med. Hyg.</italic></source><volume>89</volume><fpage>516</fpage>–<lpage>517</lpage>. <pub-id pub-id-type="doi">10.4269/ajtmh.13-0029</pub-id><pub-id pub-id-type="pmid">23878182</pub-id></mixed-citation></ref><ref id="B75"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>La Ruche</surname><given-names>G.</given-names></name><name><surname>Souares</surname><given-names>Y.</given-names></name><name><surname>Armengaud</surname><given-names>A.</given-names></name><name><surname>Peloux-Petiot</surname><given-names>F.</given-names></name><name><surname>Delaunay</surname><given-names>P.</given-names></name><name><surname>Despres</surname><given-names>P.</given-names></name><etal></etal></person-group> (<year>2010</year>). <article-title>First two autochthonous dengue virus infections in metropolitan France, September 2010.</article-title><source><italic>Euro Surveill.</italic></source><volume>15</volume>:<issue>19676</issue>.</mixed-citation></ref><ref id="B76"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lanciotti</surname><given-names>R. S.</given-names></name><name><surname>Kosoy</surname><given-names>O. L.</given-names></name><name><surname>Laven</surname><given-names>J. J.</given-names></name><name><surname>Velez</surname><given-names>J. O.</given-names></name><name><surname>Lambert</surname><given-names>A. J.</given-names></name><name><surname>Johnson</surname><given-names>A. J.</given-names></name><etal></etal></person-group> (<year>2008</year>). <article-title>Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007.</article-title><source><italic>Emerg. Infect. Dis.</italic></source><volume>14</volume><fpage>1232</fpage>–<lpage>1239</lpage>. <pub-id pub-id-type="doi">10.3201/eid1408.080287</pub-id><pub-id pub-id-type="pmid">18680646</pub-id></mixed-citation></ref><ref id="B77"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lee</surname><given-names>V. H.</given-names></name><name><surname>Moore</surname><given-names>D. L.</given-names></name></person-group> (<year>1972</year>). <article-title>Vectors of the 1969 yellow fever epidemic on the Jos Plateau, Nigeria.</article-title><source><italic>Bull. World Health Organ.</italic></source><volume>46</volume><fpage>669</fpage>–<lpage>673</lpage>.<pub-id pub-id-type="pmid">4403105</pub-id></mixed-citation></ref><ref id="B78"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Leung</surname><given-names>G. H.</given-names></name><name><surname>Baird</surname><given-names>R. W.</given-names></name><name><surname>Druce</surname><given-names>J.</given-names></name><name><surname>Anstey</surname><given-names>N. M.</given-names></name></person-group> (<year>2015</year>). <article-title>Zika virus infection in australia following a monkey bite in indonesia.</article-title><source><italic>Southeast Asian J. Trop. Med. Public Health</italic></source><volume>46</volume><fpage>460</fpage>–<lpage>464</lpage>.<pub-id pub-id-type="pmid">26521519</pub-id></mixed-citation></ref><ref id="B79"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Li</surname><given-names>J.</given-names></name><name><surname>Bhuvanakantham</surname><given-names>R.</given-names></name><name><surname>Howe</surname><given-names>J.</given-names></name><name><surname>Ng</surname><given-names>M. L.</given-names></name></person-group> (<year>2006</year>). <article-title>The glycosylation site in the envelope protein of West Nile virus (Sarafend) plays an important role in replication and maturation processes.</article-title><source><italic>J. Gen. Virol.</italic></source><volume>87</volume><fpage>613</fpage>–<lpage>622</lpage>. <pub-id pub-id-type="doi">10.1099/vir.0.81320-0</pub-id><pub-id pub-id-type="pmid">16476982</pub-id></mixed-citation></ref><ref id="B80"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Li</surname><given-names>M. I.</given-names></name><name><surname>Wong</surname><given-names>P. S.</given-names></name><name><surname>Ng</surname><given-names>L. C.</given-names></name><name><surname>Tan</surname><given-names>C. H.</given-names></name></person-group> (<year>2012</year>). <article-title>Oral susceptibility of Singapore <italic>Aedes</italic> (<italic>Stegomyia</italic>) aegypti (<italic>Linnaeus</italic>) to Zika virus.</article-title><source><italic>PLoS Negl. Trop. Dis.</italic></source><volume>6</volume>:<issue>e1792</issue><pub-id pub-id-type="doi">10.1371/journal.pntd.0001792PNTD-D-12-00044</pub-id></mixed-citation></ref><ref id="B81"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lok</surname><given-names>S. M.</given-names></name></person-group> (<year>2016</year>). <article-title>The interplay of dengue virus morphological diversity and human antibodies.</article-title><source><italic>Trends Microbiol.</italic></source><volume>24</volume><fpage>284</fpage>–<lpage>293</lpage>. <pub-id pub-id-type="doi">10.1016/j.tim.2015.12.004</pub-id><pub-id pub-id-type="pmid">26747581</pub-id></mixed-citation></ref><ref id="B82"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Luo</surname><given-names>D.</given-names></name><name><surname>Vasudevan</surname><given-names>S. G.</given-names></name><name><surname>Lescar</surname><given-names>J.</given-names></name></person-group> (<year>2015</year>). <article-title>The flavivirus NS2B-NS3 protease-helicase as a target for antiviral drug development.</article-title><source><italic>Antiviral Res.</italic></source><volume>118</volume><fpage>148</fpage>–<lpage>158</lpage>. <pub-id pub-id-type="doi">10.1016/j.antiviral.2015.03.014</pub-id><pub-id pub-id-type="pmid">25842996</pub-id></mixed-citation></ref><ref id="B83"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Macnamara</surname><given-names>F. N.</given-names></name></person-group> (<year>1954</year>). <article-title>Zika virus: a report on three cases of human infection during an epidemic of jaundice in Nigeria.</article-title><source><italic>Trans. R. Soc. Trop. Med. Hyg.</italic></source><volume>48</volume><fpage>139</fpage>–<lpage>145</lpage>. <pub-id pub-id-type="doi">10.1016/0035-9203(54)90006-1</pub-id><pub-id pub-id-type="pmid">13157159</pub-id></mixed-citation></ref><ref id="B84"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Marano</surname><given-names>G.</given-names></name><name><surname>Pupella</surname><given-names>S.</given-names></name><name><surname>Vaglio</surname><given-names>S.</given-names></name><name><surname>Liumbruno</surname><given-names>G. M.</given-names></name><name><surname>Grazzini</surname><given-names>G.</given-names></name></person-group> (<year>2016</year>). <article-title>Zika virus and the never-ending story of emerging pathogens and transfusion medicine.</article-title><source><italic>Blood Transfus.</italic></source><volume>14</volume><fpage>95</fpage>–<lpage>100</lpage>. <pub-id pub-id-type="doi">10.2450/2015.0066-15</pub-id><pub-id pub-id-type="pmid">26674815</pub-id></mixed-citation></ref><ref id="B85"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Marchette</surname><given-names>N. J.</given-names></name><name><surname>Garcia</surname><given-names>R.</given-names></name><name><surname>Rudnick</surname><given-names>A.</given-names></name></person-group> (<year>1969</year>). <article-title>Isolation of Zika virus from <italic>Aedes aegypti</italic> mosquitoes in Malaysia.</article-title><source><italic>Am. J. Trop. Med. Hyg.</italic></source><volume>18</volume><fpage>411</fpage>–<lpage>415</lpage>.<pub-id pub-id-type="pmid">4976739</pub-id></mixed-citation></ref><ref id="B86"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Marcondes</surname><given-names>C. B.</given-names></name><name><surname>Ximenes</surname><given-names>M. F.</given-names></name></person-group> (<year>2016</year>). <article-title>Zika virus in Brazil and the danger of infestation by <italic>Aedes</italic> (<italic>Stegomyia</italic>) mosquitoes.</article-title><source><italic>Rev. Soc. Bras. Med. Trop.</italic></source><pub-id pub-id-type="doi">10.1590/0037-8682-0220-2015</pub-id><comment>[Epub ahead of print]</comment>.</mixed-citation></ref><ref id="B87"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Martin-Acebes</surname><given-names>M. A.</given-names></name><name><surname>Blazquez</surname><given-names>A. B.</given-names></name><name><surname>Jimenez de Oya</surname><given-names>N.</given-names></name><name><surname>Escribano-Romero</surname><given-names>E.</given-names></name><name><surname>Saiz</surname><given-names>J. C.</given-names></name></person-group> (<year>2011</year>). <article-title>West nile virus replication requires Fatty Acid synthesis but is independent on phosphatidylinositol-4-phosphate lipids.</article-title><source><italic>PLoS ONE</italic></source><volume>6</volume>:<issue>e24970</issue><pub-id pub-id-type="doi">10.1371/journal.pone.0024970</pub-id></mixed-citation></ref><ref id="B88"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Martin-Acebes</surname><given-names>M. A.</given-names></name><name><surname>Saiz</surname><given-names>J. C.</given-names></name></person-group> (<year>2012</year>). <article-title>West Nile virus: a re-emerging pathogen revisited.</article-title><source><italic>World J. Virol.</italic></source><volume>1</volume><fpage>51</fpage>–<lpage>70</lpage>. <pub-id pub-id-type="doi">10.5501/wjv.v1.i2.51</pub-id><pub-id pub-id-type="pmid">24175211</pub-id></mixed-citation></ref><ref id="B89"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Martines</surname><given-names>R. B.</given-names></name><name><surname>Bhatnagar</surname><given-names>J.</given-names></name><name><surname>Keating</surname><given-names>M. K.</given-names></name><name><surname>Silva-Flannery</surname><given-names>L.</given-names></name><name><surname>Muehlenbachs</surname><given-names>A.</given-names></name><name><surname>Gary</surname><given-names>J.</given-names></name><etal></etal></person-group> (<year>2016</year>). <article-title>Notes from the field: evidence of zika virus infection in brain and placental tissues from two congenitally infected newborns and two fetal losses - Brazil, 2015.</article-title><source><italic>Morb. Mortal. Wkly. Rep.</italic></source><volume>65</volume><fpage>159</fpage>–<lpage>160</lpage>. <pub-id pub-id-type="doi">10.15585/mmwr.mm6506e1</pub-id></mixed-citation></ref><ref id="B90"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>McCarthy</surname><given-names>M.</given-names></name></person-group> (<year>2016</year>). <article-title>Zika virus was transmitted by sexual contact in Texas, health officials report.</article-title><source><italic>BMJ</italic></source><volume>352</volume><issue>i720</issue><pub-id pub-id-type="doi">10.1136/bmj.i720</pub-id></mixed-citation></ref><ref id="B91"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>McCrae</surname><given-names>A. W.</given-names></name><name><surname>Kirya</surname><given-names>B. G.</given-names></name></person-group> (<year>1982</year>). <article-title>Yellow fever and Zika virus epizootics and enzootics in Uganda.</article-title><source><italic>Trans. R. Soc. Trop. Med. Hyg.</italic></source><volume>76</volume><fpage>552</fpage>–<lpage>562</lpage>. <pub-id pub-id-type="doi">10.1016/0035-9203(82)90161-4</pub-id><pub-id pub-id-type="pmid">6304948</pub-id></mixed-citation></ref><ref id="B92"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Medlock</surname><given-names>J. M.</given-names></name><name><surname>Hansford</surname><given-names>K. M.</given-names></name><name><surname>Schaffner</surname><given-names>F.</given-names></name><name><surname>Versteirt</surname><given-names>V.</given-names></name><name><surname>Hendrickx</surname><given-names>G.</given-names></name><name><surname>Zeller</surname><given-names>H.</given-names></name><etal></etal></person-group> (<year>2012</year>). <article-title>A review of the invasive mosquitoes in Europe: ecology, public health risks, and control options.</article-title><source><italic>Vector Borne Zoonotic Dis.</italic></source><volume>12</volume><fpage>435</fpage>–<lpage>447</lpage>. <pub-id pub-id-type="doi">10.1089/vbz.2011.0814</pub-id><pub-id pub-id-type="pmid">22448724</pub-id></mixed-citation></ref><ref id="B93"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Miorin</surname><given-names>L.</given-names></name><name><surname>Romero-Brey</surname><given-names>I.</given-names></name><name><surname>Maiuri</surname><given-names>P.</given-names></name><name><surname>Hoppe</surname><given-names>S.</given-names></name><name><surname>Krijnse-Locker</surname><given-names>J.</given-names></name><name><surname>Bartenschlager</surname><given-names>R.</given-names></name><etal></etal></person-group> (<year>2013</year>). <article-title>Three-dimensional architecture of tick-borne encephalitis virus replication sites and trafficking of the replicated RNA.</article-title><source><italic>J. Virol.</italic></source><volume>87</volume><fpage>6469</fpage>–<lpage>6481</lpage>. <pub-id pub-id-type="doi">10.1128/JVI.03456-12</pub-id><pub-id pub-id-type="pmid">23552408</pub-id></mixed-citation></ref><ref id="B94"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Mlakar</surname><given-names>J.</given-names></name><name><surname>Korva</surname><given-names>M.</given-names></name><name><surname>Tul</surname><given-names>N.</given-names></name><name><surname>Popovic</surname><given-names>M.</given-names></name><name><surname>Poljsak-Prijatelj</surname><given-names>M.</given-names></name><name><surname>Mraz</surname><given-names>J.</given-names></name><etal></etal></person-group> (<year>2016</year>). <article-title>Zika Virus associated with microcephaly.</article-title><source><italic>N. Eng. J. Med.</italic></source><volume>374</volume><fpage>951</fpage>–<lpage>958</lpage>. <pub-id pub-id-type="doi">10.1056/NEJMoa1600651</pub-id></mixed-citation></ref><ref id="B95"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Monath</surname><given-names>T. P.</given-names></name><name><surname>Vasconcelos</surname><given-names>P. F.</given-names></name></person-group> (<year>2015</year>). <article-title>Yellow fever.</article-title><source><italic>J. Clin. Virol.</italic></source><volume>64</volume><fpage>160</fpage>–<lpage>173</lpage>. <pub-id pub-id-type="doi">10.1016/j.jcv.2014.08.030S1386-6532(14)00369-2</pub-id><pub-id pub-id-type="pmid">25453327</pub-id></mixed-citation></ref><ref id="B96"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Monath</surname><given-names>T. P.</given-names></name><name><surname>Wilson</surname><given-names>D. C.</given-names></name><name><surname>Lee</surname><given-names>V. H.</given-names></name><name><surname>Stroh</surname><given-names>G.</given-names></name><name><surname>Kuteyi</surname><given-names>K.</given-names></name><name><surname>Smith</surname><given-names>E. A.</given-names></name></person-group> (<year>1973</year>). <article-title>The 1970 yellow fever epidemic in Okwoga District, Benue Plateau State, Nigeria. I. Epidemiological observations.</article-title><source><italic>Bull. World Health Organ.</italic></source><volume>49</volume><fpage>113</fpage>–<lpage>121</lpage>.<pub-id pub-id-type="pmid">4545318</pub-id></mixed-citation></ref><ref id="B97"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Monlun</surname><given-names>E.</given-names></name><name><surname>Zeller</surname><given-names>H.</given-names></name><name><surname>Le Guenno</surname><given-names>B.</given-names></name><name><surname>Traore-Lamizana</surname><given-names>M.</given-names></name><name><surname>Hervy</surname><given-names>J. P.</given-names></name><name><surname>Adam</surname><given-names>F.</given-names></name><etal></etal></person-group> (<year>1993</year>). <article-title>[Surveillance of the circulation of arbovirus of medical interest in the region of eastern Senegal].</article-title><source><italic>Bull. Soc. Pathol. Exot.</italic></source><volume>86</volume><fpage>21</fpage>–<lpage>28</lpage>.<pub-id pub-id-type="pmid">8099299</pub-id></mixed-citation></ref><ref id="B98"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Mukhopadhyay</surname><given-names>S.</given-names></name><name><surname>Kim</surname><given-names>B. S.</given-names></name><name><surname>Chipman</surname><given-names>P. R.</given-names></name><name><surname>Rossmann</surname><given-names>M. G.</given-names></name><name><surname>Kuhn</surname><given-names>R. J.</given-names></name></person-group> (<year>2003</year>). <article-title>Structure of West Nile virus.</article-title><source><italic>Science</italic></source><volume>302</volume><issue>248</issue><pub-id pub-id-type="doi">10.1126/science.1089316</pub-id></mixed-citation></ref><ref id="B99"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Mukhopadhyay</surname><given-names>S.</given-names></name><name><surname>Kuhn</surname><given-names>R. J.</given-names></name><name><surname>Rossmann</surname><given-names>M. G.</given-names></name></person-group> (<year>2005</year>). <article-title>A structural perspective of the flavivirus life cycle.</article-title><source><italic>Nat. Rev. Microbiol.</italic></source><volume>3</volume><fpage>13</fpage>–<lpage>22</lpage>. <pub-id pub-id-type="doi">10.1038/nrmicro1067</pub-id><pub-id pub-id-type="pmid">15608696</pub-id></mixed-citation></ref><ref id="B100"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Musso</surname><given-names>D.</given-names></name></person-group> (<year>2015</year>). <article-title>Zika virus transmission from french polynesia to Brazil.</article-title><source><italic>Emerg. Infect. Dis.</italic></source><volume>21</volume>:<issue>1887</issue><pub-id pub-id-type="doi">10.3201/eid2110.151125</pub-id></mixed-citation></ref><ref id="B101"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Musso</surname><given-names>D.</given-names></name><name><surname>Nhan</surname><given-names>T.</given-names></name><name><surname>Robin</surname><given-names>E.</given-names></name><name><surname>Roche</surname><given-names>C.</given-names></name><name><surname>Bierlaire</surname><given-names>D.</given-names></name><name><surname>Zisou</surname><given-names>K.</given-names></name><etal></etal></person-group> (<year>2014a</year>). <article-title>Potential for Zika virus transmission through blood transfusion demonstrated during an outbreak in French Polynesia, November 2013 to February 2014.</article-title><source><italic>Euro Surveill.</italic></source><volume>19</volume>:<issue>20761</issue><pub-id pub-id-type="doi">10.2807/1560-7917.ES2014.19.14.20761</pub-id></mixed-citation></ref><ref id="B102"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Musso</surname><given-names>D.</given-names></name><name><surname>Nilles</surname><given-names>E. J.</given-names></name><name><surname>Cao-Lormeau</surname><given-names>V. M.</given-names></name></person-group> (<year>2014b</year>). <article-title>Rapid spread of emerging Zika virus in the Pacific area.</article-title><source><italic>Clin. Microbiol. Infect.</italic></source><volume>20</volume><fpage>O595</fpage>–<lpage>O596</lpage>. <pub-id pub-id-type="doi">10.1111/1469-0691.12707</pub-id><pub-id pub-id-type="pmid">24909208</pub-id></mixed-citation></ref><ref id="B103"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Musso</surname><given-names>D.</given-names></name><name><surname>Roche</surname><given-names>C.</given-names></name><name><surname>Nhan</surname><given-names>T. X.</given-names></name><name><surname>Robin</surname><given-names>E.</given-names></name><name><surname>Teissier</surname><given-names>A.</given-names></name><name><surname>Cao-Lormeau</surname><given-names>V. M.</given-names></name></person-group> (<year>2015a</year>). <article-title>Detection of Zika virus in saliva.</article-title><source><italic>J. Clin. Virol.</italic></source><volume>68</volume><fpage>53</fpage>–<lpage>55</lpage>. <pub-id pub-id-type="doi">10.1016/j.jcv.2015.04.021S1386-6532(15)00133-X</pub-id><pub-id pub-id-type="pmid">26071336</pub-id></mixed-citation></ref><ref id="B104"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Musso</surname><given-names>D.</given-names></name><name><surname>Roche</surname><given-names>C.</given-names></name><name><surname>Robin</surname><given-names>E.</given-names></name><name><surname>Nhan</surname><given-names>T.</given-names></name><name><surname>Teissier</surname><given-names>A.</given-names></name><name><surname>Cao-Lormeau</surname><given-names>V. M.</given-names></name></person-group> (<year>2015b</year>). <article-title>Potential sexual transmission of Zika virus.</article-title><source><italic>Emerg. Infect. Dis.</italic></source><volume>21</volume><fpage>359</fpage>–<lpage>361</lpage>. <pub-id pub-id-type="doi">10.3201/eid2102.141363</pub-id><pub-id pub-id-type="pmid">25625872</pub-id></mixed-citation></ref><ref id="B105"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Noble</surname><given-names>C. G.</given-names></name><name><surname>Shi</surname><given-names>P. Y.</given-names></name></person-group> (<year>2012</year>). <article-title>Structural biology of dengue virus enzymes: towards rational design of therapeutics.</article-title><source><italic>Antiviral Res.</italic></source><volume>96</volume><fpage>115</fpage>–<lpage>126</lpage>. <pub-id pub-id-type="doi">10.1016/j.antiviral.2012.09.007</pub-id><pub-id pub-id-type="pmid">22995600</pub-id></mixed-citation></ref><ref id="B106"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Oehler</surname><given-names>E.</given-names></name><name><surname>Watrin</surname><given-names>L.</given-names></name><name><surname>Larre</surname><given-names>P.</given-names></name><name><surname>Leparc-Goffart</surname><given-names>I.</given-names></name><name><surname>Lastere</surname><given-names>S.</given-names></name><name><surname>Valour</surname><given-names>F.</given-names></name><etal></etal></person-group> (<year>2014</year>). <article-title>Zika virus infection complicated by Guillain-Barre syndrome–case report, French Polynesia, December 2013.</article-title><source><italic>Euro Surveill.</italic></source><volume>19</volume>:<issue>20720</issue><pub-id pub-id-type="doi">10.2807/1560-7917.ES2014.19.9.20720</pub-id></mixed-citation></ref><ref id="B107"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Oliveira Melo</surname><given-names>A. S.</given-names></name><name><surname>Malinger</surname><given-names>G.</given-names></name><name><surname>Ximenes</surname><given-names>R.</given-names></name><name><surname>Szejnfeld</surname><given-names>P. O.</given-names></name><name><surname>Alves Sampaio</surname><given-names>S.</given-names></name><name><surname>Bispo de Filippis</surname><given-names>A. M.</given-names></name></person-group> (<year>2016</year>). <article-title>Zika virus intrauterine infection causes fetal brain abnormality and microcephaly: tip of the iceberg?</article-title><source><italic>Ultrasound Obstet Gynecol.</italic></source><volume>47</volume><fpage>6</fpage>–<lpage>7</lpage>. <pub-id pub-id-type="doi">10.1002/uog.15831</pub-id><pub-id pub-id-type="pmid">26731034</pub-id></mixed-citation></ref><ref id="B108"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Olson</surname><given-names>J. G.</given-names></name><name><surname>Ksiazek</surname><given-names>T. G.</given-names></name><name><surname>Gubler</surname><given-names>D. J.</given-names></name><name><surname>Lubis</surname><given-names>S. I.</given-names></name><name><surname>Simanjuntak</surname><given-names>G.</given-names></name><name><surname>Lee</surname><given-names>V. H.</given-names></name><etal></etal></person-group> (<year>1983</year>). <article-title>A survey for arboviral antibodies in sera of humans and animals in Lombok, Republic of Indonesia.</article-title><source><italic>Ann. Trop. Med. Parasitol.</italic></source><volume>77</volume><fpage>131</fpage>–<lpage>137</lpage>.<pub-id pub-id-type="pmid">6309104</pub-id></mixed-citation></ref><ref id="B109"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Olson</surname><given-names>J. G.</given-names></name><name><surname>Ksiazek</surname><given-names>T. G.</given-names></name><name><surname>Suhandiman</surname></name><name><surname>Triwibowo</surname></name></person-group> (<year>1981</year>). <article-title>Zika virus, a cause of fever in Central Java, Indonesia.</article-title><source><italic>Trans. R. Soc. Trop. Med. Hyg.</italic></source><volume>75</volume><fpage>389</fpage>–<lpage>393</lpage>. <pub-id pub-id-type="doi">10.1016/0035-9203(81)90100-0</pub-id><pub-id pub-id-type="pmid">6275577</pub-id></mixed-citation></ref><ref id="B110"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Paupy</surname><given-names>C.</given-names></name><name><surname>Delatte</surname><given-names>H.</given-names></name><name><surname>Bagny</surname><given-names>L.</given-names></name><name><surname>Corbel</surname><given-names>V.</given-names></name><name><surname>Fontenille</surname><given-names>D.</given-names></name></person-group> (<year>2009</year>). <article-title><italic>Aedes albopictus</italic>, an arbovirus vector: from the darkness to the light.</article-title><source><italic>Microbes Infect.</italic></source><volume>11</volume><fpage>1177</fpage>–<lpage>1185</lpage>. <pub-id pub-id-type="doi">10.1016/j.micinf.2009.05.005S1286-4579(09)00105-1</pub-id><pub-id pub-id-type="pmid">19450706</pub-id></mixed-citation></ref><ref id="B111"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Petersen</surname><given-names>E.</given-names></name><name><surname>Wilson</surname><given-names>M. E.</given-names></name><name><surname>Touch</surname><given-names>S.</given-names></name><name><surname>McCloskey</surname><given-names>B.</given-names></name><name><surname>Mwaba</surname><given-names>P.</given-names></name><name><surname>Bates</surname><given-names>M.</given-names></name><etal></etal></person-group> (<year>2016</year>). <article-title>Rapid spread of Zika virus in the Americas – implications for public health preparedness for mass gatherings at the 2016 Brazil Olympic Games.</article-title><source><italic>Int. J. Infect. Dis.</italic></source><volume>44</volume><fpage>11</fpage>–<lpage>15</lpage>. <pub-id pub-id-type="doi">10.1016/j.ijid.2016.02.001</pub-id><pub-id pub-id-type="pmid">26854199</pub-id></mixed-citation></ref><ref id="B112"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pierson</surname><given-names>T. C.</given-names></name><name><surname>Diamond</surname><given-names>M. S.</given-names></name></person-group> (<year>2012</year>). <article-title>Degrees of maturity: the complex structure and biology of flaviviruses.</article-title><source><italic>Curr. Opin. Virol.</italic></source><volume>2</volume><fpage>168</fpage>–<lpage>175</lpage>. <pub-id pub-id-type="doi">10.1016/j.coviro.2012.02.011</pub-id><pub-id pub-id-type="pmid">22445964</pub-id></mixed-citation></ref><ref id="B113"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Plevka</surname><given-names>P.</given-names></name><name><surname>Battisti</surname><given-names>A. J.</given-names></name><name><surname>Junjhon</surname><given-names>J.</given-names></name><name><surname>Winkler</surname><given-names>D. C.</given-names></name><name><surname>Holdaway</surname><given-names>H. A.</given-names></name><name><surname>Keelapang</surname><given-names>P.</given-names></name><etal></etal></person-group> (<year>2011</year>). <article-title>Maturation of flaviviruses starts from one or more icosahedrally independent nucleation centres.</article-title><source><italic>EMBO Rep.</italic></source><volume>12</volume><fpage>602</fpage>–<lpage>606</lpage>. <pub-id pub-id-type="doi">10.1038/embor.2011.75</pub-id><pub-id pub-id-type="pmid">21566648</pub-id></mixed-citation></ref><ref id="B114"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pond</surname><given-names>W. L.</given-names></name></person-group> (<year>1963</year>). <article-title>Arthropod-borne virus antibodies in sera from residents of South-East Asia.</article-title><source><italic>Trans. R. Soc. Trop. Med. Hyg.</italic></source><volume>57</volume><fpage>364</fpage>–<lpage>371</lpage>. <pub-id pub-id-type="doi">10.1016/0035-9203(63)90100-7</pub-id><pub-id pub-id-type="pmid">14062273</pub-id></mixed-citation></ref><ref id="B115"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ponlawat</surname><given-names>A.</given-names></name><name><surname>Harrington</surname><given-names>L. C.</given-names></name></person-group> (<year>2005</year>). <article-title>Blood feeding patterns of <italic>Aedes aegypti</italic> and <italic>Aedes albopictus</italic> in Thailand.</article-title><source><italic>J. Med. Entomol.</italic></source><volume>42</volume><fpage>844</fpage>–<lpage>849</lpage>. <pub-id pub-id-type="doi">10.1603/0022-2585(2005)042[0821:ISOAAA]2.0.CO;2</pub-id><pub-id pub-id-type="pmid">16363170</pub-id></mixed-citation></ref><ref id="B116"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Puccioni-Sohler</surname><given-names>M.</given-names></name><name><surname>Orsini</surname><given-names>M.</given-names></name><name><surname>Soares</surname><given-names>C. N.</given-names></name></person-group> (<year>2012</year>). <article-title>Dengue: a new challenge for neurology.</article-title><source><italic>Neurol. Int.</italic></source><volume>4</volume>:<issue>e15</issue><pub-id pub-id-type="doi">10.4081/ni.2012.e15ni.2012.e15</pub-id></mixed-citation></ref><ref id="B117"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pupella</surname><given-names>S.</given-names></name><name><surname>Pisani</surname><given-names>G.</given-names></name><name><surname>Cristiano</surname><given-names>K.</given-names></name><name><surname>Catalano</surname><given-names>L.</given-names></name><name><surname>Grazzini</surname><given-names>G.</given-names></name></person-group> (<year>2013</year>). <article-title>West Nile virus in the transfusion setting with a special focus on Italian preventive measures adopted in 2008-2012 and their impact on blood safety.</article-title><source><italic>Blood Transfus.</italic></source><volume>11</volume><fpage>563</fpage>–<lpage>574</lpage>. <pub-id pub-id-type="doi">10.2450/2013.0077-132013.0077-13</pub-id><pub-id pub-id-type="pmid">24120610</pub-id></mixed-citation></ref><ref id="B118"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Reiter</surname><given-names>P.</given-names></name></person-group> (<year>2010</year>). <article-title>Yellow fever and dengue: a threat to Europe?</article-title><source><italic>Euro Surveill.</italic></source><volume>15</volume>:<issue>19509</issue>.</mixed-citation></ref><ref id="B119"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rezza</surname><given-names>G.</given-names></name><name><surname>Nicoletti</surname><given-names>L.</given-names></name><name><surname>Angelini</surname><given-names>R.</given-names></name><name><surname>Romi</surname><given-names>R.</given-names></name><name><surname>Finarelli</surname><given-names>A. C.</given-names></name><name><surname>Panning</surname><given-names>M.</given-names></name><etal></etal></person-group> (<year>2007</year>). <article-title>Infection with chikungunya virus in Italy: an outbreak in a temperate region.</article-title><source><italic>Lancet</italic></source><volume>370</volume><fpage>1840</fpage>–<lpage>1846</lpage>. <pub-id pub-id-type="doi">10.1016/S0140-6736(07)61779-6</pub-id><pub-id pub-id-type="pmid">18061059</pub-id></mixed-citation></ref><ref id="B120"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Robin</surname><given-names>Y.</given-names></name><name><surname>Mouchet</surname><given-names>J.</given-names></name></person-group> (<year>1975</year>). <article-title>[Serological and entomological study on yellow fever in Sierra Leone].</article-title><source><italic>Bull. Soc. Pathol. Exot. Fil.</italic></source><volume>68</volume><fpage>249</fpage>–<lpage>258</lpage>.</mixed-citation></ref><ref id="B121"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Roby</surname><given-names>J. A.</given-names></name><name><surname>Setoh</surname><given-names>Y. X.</given-names></name><name><surname>Hall</surname><given-names>R. A.</given-names></name><name><surname>Khromykh</surname><given-names>A. A.</given-names></name></person-group> (<year>2015</year>). <article-title>Post-translational regulation and modifications of flavivirus structural proteins.</article-title><source><italic>J. Gen. Virol.</italic></source><volume>96</volume><fpage>1551</fpage>–<lpage>1569</lpage>. <pub-id pub-id-type="doi">10.1099/vir.0.000097vir.0.000097</pub-id><pub-id pub-id-type="pmid">25711963</pub-id></mixed-citation></ref><ref id="B122"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Roehrig</surname><given-names>J. T.</given-names></name><name><surname>Staudinger</surname><given-names>L. A.</given-names></name><name><surname>Hunt</surname><given-names>A. R.</given-names></name><name><surname>Mathews</surname><given-names>J. H.</given-names></name><name><surname>Blair</surname><given-names>C. D.</given-names></name></person-group> (<year>2001</year>). <article-title>Antibody prophylaxis and therapy for flavivirus encephalitis infections.</article-title><source><italic>Ann. N. Y. Acad. Sci.</italic></source><volume>951</volume><fpage>286</fpage>–<lpage>297</lpage>. <pub-id pub-id-type="doi">10.1111/j.1749-6632.2001.tb02704.x</pub-id><pub-id pub-id-type="pmid">11797785</pub-id></mixed-citation></ref><ref id="B123"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Roth</surname><given-names>A.</given-names></name><name><surname>Mercier</surname><given-names>A.</given-names></name><name><surname>Lepers</surname><given-names>C.</given-names></name><name><surname>Hoy</surname><given-names>D.</given-names></name><name><surname>Duituturaga</surname><given-names>S.</given-names></name><name><surname>Benyon</surname><given-names>E.</given-names></name><etal></etal></person-group> (<year>2014</year>). <article-title>Concurrent outbreaks of dengue, chikungunya and Zika virus infections - an unprecedented epidemic wave of mosquito-borne viruses in the Pacific 2012-2014.</article-title><source><italic>Euro Surveill.</italic></source><volume>19</volume>:<issue>20929</issue><pub-id pub-id-type="doi">10.2807/1560-7917.ES2014.19.41.20929</pub-id></mixed-citation></ref><ref id="B124"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rubin</surname><given-names>E. J.</given-names></name><name><surname>Greene</surname><given-names>M. F.</given-names></name><name><surname>Baden</surname><given-names>L. R.</given-names></name></person-group> (<year>2016</year>). <article-title>Zika virus and microcephaly.</article-title><source><italic>N. Engl. J. Med.</italic></source><pub-id pub-id-type="doi">10.1056/NEJMe1601862</pub-id><comment>[Epub ahead of print]</comment>.</mixed-citation></ref><ref id="B125"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Saluzzo</surname><given-names>J. F.</given-names></name><name><surname>Gonzalez</surname><given-names>J. P.</given-names></name><name><surname>Herve</surname><given-names>J. P.</given-names></name><name><surname>Georges</surname><given-names>A. J.</given-names></name></person-group> (<year>1981</year>). <article-title>[Serological survey for the prevalence of certain arboviruses in the human population of the south-east area of Central African Republic (author’s transl)].</article-title><source><italic>Bull. Soc. Pathol. Exot. Fil.</italic></source><volume>74</volume><fpage>490</fpage>–<lpage>499</lpage>.</mixed-citation></ref><ref id="B126"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Schuler-Faccini</surname><given-names>L.</given-names></name><name><surname>Ribeiro</surname><given-names>E. M.</given-names></name><name><surname>Feitosa</surname><given-names>I. M.</given-names></name><name><surname>Horovitz</surname><given-names>D. D.</given-names></name><name><surname>Cavalcanti</surname><given-names>D. P.</given-names></name><name><surname>Pessoa</surname><given-names>A.</given-names></name><etal></etal></person-group> (<year>2016</year>). <article-title>Possible association between zika virus infection and microcephaly - Brazil, 2015.</article-title><source><italic>Morb. Mortal. Wkly. Rep.</italic></source><volume>65</volume><fpage>59</fpage>–<lpage>62</lpage>. <pub-id pub-id-type="doi">10.15585/mmwr.mm6503e2</pub-id></mixed-citation></ref><ref id="B127"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Scott</surname><given-names>T. W.</given-names></name><name><surname>Takken</surname><given-names>W.</given-names></name></person-group> (<year>2012</year>). <article-title>Feeding strategies of anthropophilic mosquitoes result in increased risk of pathogen transmission.</article-title><source><italic>Trends Parasitol.</italic></source><volume>28</volume><fpage>114</fpage>–<lpage>121</lpage>. <pub-id pub-id-type="doi">10.1016/j.pt.2012.01.001S1471-4922(12)00004-9</pub-id><pub-id pub-id-type="pmid">22300806</pub-id></mixed-citation></ref><ref id="B128"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Shinohara</surname><given-names>K.</given-names></name><name><surname>Kutsuna</surname><given-names>S.</given-names></name><name><surname>Takasaki</surname><given-names>T.</given-names></name><name><surname>Moi</surname><given-names>M. L.</given-names></name><name><surname>Ikeda</surname><given-names>M.</given-names></name><name><surname>Kotaki</surname><given-names>A.</given-names></name><etal></etal></person-group> (<year>2016</year>). <article-title>Zika fever imported from Thailand to Japan, and diagnosed by PCR in the urines.</article-title><source><italic>J. Travel Med.</italic></source><volume>23</volume>:<issue>tav011</issue><pub-id pub-id-type="doi">10.1093/jtm/tav011tav011</pub-id></mixed-citation></ref><ref id="B129"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Shirato</surname><given-names>K.</given-names></name><name><surname>Miyoshi</surname><given-names>H.</given-names></name><name><surname>Goto</surname><given-names>A.</given-names></name><name><surname>Ako</surname><given-names>Y.</given-names></name><name><surname>Ueki</surname><given-names>T.</given-names></name><name><surname>Kariwa</surname><given-names>H.</given-names></name><etal></etal></person-group> (<year>2004</year>). <article-title>Viral envelope protein glycosylation is a molecular determinant of the neuroinvasiveness of the New York strain of West Nile virus.</article-title><source><italic>J. Gen. Virol.</italic></source><volume>85</volume><fpage>3637</fpage>–<lpage>3645</lpage>. <pub-id pub-id-type="doi">10.1099/vir.0.80247-0</pub-id><pub-id pub-id-type="pmid">15557236</pub-id></mixed-citation></ref><ref id="B130"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Simpson</surname><given-names>D. I.</given-names></name></person-group> (<year>1964</year>). <article-title>Zika virus Infection in Man.</article-title><source><italic>Trans. R. Soc. Trop. Med. Hyg.</italic></source><volume>58</volume><fpage>335</fpage>–<lpage>338</lpage>. <pub-id pub-id-type="doi">10.1016/0035-9203(64)90201-9</pub-id><pub-id pub-id-type="pmid">14175744</pub-id></mixed-citation></ref><ref id="B131"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Smithburn</surname><given-names>K. C.</given-names></name></person-group> (<year>1952</year>). <article-title>Neutralizing antibodies against certain recently isolated viruses in the sera of human beings residing in East Africa.</article-title><source><italic>J. Immunol.</italic></source><volume>69</volume><fpage>223</fpage>–<lpage>234</lpage>.<pub-id pub-id-type="pmid">14946416</pub-id></mixed-citation></ref><ref id="B132"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Smithburn</surname><given-names>K. C.</given-names></name></person-group> (<year>1954</year>). <article-title>Neutralizing antibodies against arthropod-borne viruses in the sera of long-time residents of Malaya and Borneo.</article-title><source><italic>Am. J. Hyg.</italic></source><volume>59</volume><fpage>157</fpage>–<lpage>163</lpage>.<pub-id pub-id-type="pmid">13138582</pub-id></mixed-citation></ref><ref id="B133"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Smithburn</surname><given-names>K. C.</given-names></name><name><surname>Kerr</surname><given-names>J. A.</given-names></name><name><surname>Gatne</surname><given-names>P. B.</given-names></name></person-group> (<year>1954</year>). <article-title>Neutralizing antibodies against certain viruses in the sera of residents of India.</article-title><source><italic>J. Immunol.</italic></source><volume>72</volume><fpage>248</fpage>–<lpage>257</lpage>.<pub-id pub-id-type="pmid">13163397</pub-id></mixed-citation></ref><ref id="B134"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Staples</surname><given-names>J. E.</given-names></name><name><surname>Dziuban</surname><given-names>E. J.</given-names></name><name><surname>Fischer</surname><given-names>M.</given-names></name><name><surname>Cragan</surname><given-names>J. D.</given-names></name><name><surname>Rasmussen</surname><given-names>S. A.</given-names></name><name><surname>Cannon</surname><given-names>M. J.</given-names></name><etal></etal></person-group> (<year>2016</year>). <article-title>Interim guidelines for the evaluation and testing of infants with possible congenital zika virus infection - United States, 2016.</article-title><source><italic>Morb. Mortal. Wkly. Rep.</italic></source><volume>65</volume><fpage>63</fpage>–<lpage>67</lpage>. <pub-id pub-id-type="doi">10.15585/mmwr.mm6503e3</pub-id></mixed-citation></ref><ref id="B135"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Stiasny</surname><given-names>K.</given-names></name><name><surname>Fritz</surname><given-names>R.</given-names></name><name><surname>Pangerl</surname><given-names>K.</given-names></name><name><surname>Heinz</surname><given-names>F. X.</given-names></name></person-group> (<year>2011</year>). <article-title>Molecular mechanisms of flavivirus membrane fusion.</article-title><source><italic>Amino Acids</italic></source><volume>41</volume><fpage>1159</fpage>–<lpage>1163</lpage>. <pub-id pub-id-type="doi">10.1007/s00726-009-0370-4</pub-id><pub-id pub-id-type="pmid">19882217</pub-id></mixed-citation></ref><ref id="B136"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tappe</surname><given-names>D.</given-names></name><name><surname>Nachtigall</surname><given-names>S.</given-names></name><name><surname>Kapaun</surname><given-names>A.</given-names></name><name><surname>Schnitzler</surname><given-names>P.</given-names></name><name><surname>Gunther</surname><given-names>S.</given-names></name><name><surname>Schmidt-Chanasit</surname><given-names>J.</given-names></name></person-group> (<year>2015</year>). <article-title>Acute Zika virus infection after travel to Malaysian Borneo, September 2014.</article-title><source><italic>Emerg. Infect. Dis.</italic></source><volume>21</volume><fpage>911</fpage>–<lpage>913</lpage>. <pub-id pub-id-type="doi">10.3201/eid2105.141960</pub-id><pub-id pub-id-type="pmid">25898277</pub-id></mixed-citation></ref><ref id="B137"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tappe</surname><given-names>D.</given-names></name><name><surname>Rissland</surname><given-names>J.</given-names></name><name><surname>Gabriel</surname><given-names>M.</given-names></name><name><surname>Emmerich</surname><given-names>P.</given-names></name><name><surname>Gunther</surname><given-names>S.</given-names></name><name><surname>Held</surname><given-names>G.</given-names></name><etal></etal></person-group> (<year>2014</year>). <article-title>First case of laboratory-confirmed Zika virus infection imported into Europe, November 2013.</article-title><source><italic>Euro Surveill.</italic></source><volume>19</volume>:<issue>20685</issue><pub-id pub-id-type="doi">10.2807/1560-7917.ES2014.19.4.20685</pub-id></mixed-citation></ref><ref id="B138"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tetro</surname><given-names>J. A.</given-names></name></person-group> (<year>2016</year>). <article-title>Zika and microcephaly: causation, correlation, or coincidence?</article-title><source><italic>Microb. Infect.</italic></source><volume>18</volume><fpage>167</fpage>–<lpage>168</lpage>. <pub-id pub-id-type="doi">10.1016/j.micinf.2015.12.010</pub-id></mixed-citation></ref><ref id="B139"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Thomas</surname><given-names>S. M.</given-names></name><name><surname>Obermayr</surname><given-names>U.</given-names></name><name><surname>Fischer</surname><given-names>D.</given-names></name><name><surname>Kreyling</surname><given-names>J.</given-names></name><name><surname>Beierkuhnlein</surname><given-names>C.</given-names></name></person-group> (<year>2012</year>). <article-title>Low-temperature threshold for egg survival of a post-diapause and non-diapause European aedine strain, <italic>Aedes albopictus</italic> (Diptera: Culicidae).</article-title><source><italic>Parasit. Vectors</italic></source><volume>5</volume>:<issue>100</issue><pub-id pub-id-type="doi">10.1186/1756-3305-5-100</pub-id></mixed-citation></ref><ref id="B140"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tognarelli</surname><given-names>J.</given-names></name><name><surname>Ulloa</surname><given-names>S.</given-names></name><name><surname>Villagra</surname><given-names>E.</given-names></name><name><surname>Lagos</surname><given-names>J.</given-names></name><name><surname>Aguayo</surname><given-names>C.</given-names></name><name><surname>Fasce</surname><given-names>R.</given-names></name><etal></etal></person-group> (<year>2016</year>). <article-title>A report on the outbreak of Zika virus on Easter Island, South Pacific, 2014.</article-title><source><italic>Arch. Virol.</italic></source><volume>161</volume><fpage>665</fpage>–<lpage>668</lpage>. <pub-id pub-id-type="doi">10.1007/s00705-015-2695-5</pub-id><pub-id pub-id-type="pmid">26611910</pub-id></mixed-citation></ref><ref id="B141"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ulbert</surname><given-names>S.</given-names></name><name><surname>Magnusson</surname><given-names>S. E.</given-names></name></person-group> (<year>2014</year>). <article-title>Technologies for the development of West Nile virus vaccines.</article-title><source><italic>Future Microbiol.</italic></source><volume>9</volume><fpage>1221</fpage>–<lpage>1232</lpage>. <pub-id pub-id-type="doi">10.2217/fmb.14.67</pub-id><pub-id pub-id-type="pmid">25405890</pub-id></mixed-citation></ref><ref id="B142"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>van den Berg</surname><given-names>B.</given-names></name><name><surname>Bunschoten</surname><given-names>C.</given-names></name><name><surname>van Doorn</surname><given-names>P. A.</given-names></name><name><surname>Jacobs</surname><given-names>B. C.</given-names></name></person-group> (<year>2013</year>). <article-title>Mortality in guillain-barre syndrome.</article-title><source><italic>Neurology</italic></source><volume>80</volume><fpage>1650</fpage>–<lpage>1654</lpage>. <pub-id pub-id-type="doi">10.1212/WNL.0b013e3182904fcc</pub-id><pub-id pub-id-type="pmid">23576619</pub-id></mixed-citation></ref><ref id="B143"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Vazquez-Calvo</surname><given-names>A.</given-names></name><name><surname>Saiz</surname><given-names>J. C.</given-names></name><name><surname>McCullough</surname><given-names>K. C.</given-names></name><name><surname>Sobrino</surname><given-names>F.</given-names></name><name><surname>Martin-Acebes</surname><given-names>M. A.</given-names></name></person-group> (<year>2012</year>). <article-title>Acid-dependent viral entry.</article-title><source><italic>Virus Res.</italic></source><volume>167</volume><fpage>125</fpage>–<lpage>137</lpage>. <pub-id pub-id-type="doi">10.1016/j.virusres.2012.05.024</pub-id><pub-id pub-id-type="pmid">22683298</pub-id></mixed-citation></ref><ref id="B144"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ventura</surname><given-names>C. V.</given-names></name><name><surname>Maia</surname><given-names>M.</given-names></name><name><surname>Bravo-Filho</surname><given-names>V.</given-names></name><name><surname>Gois</surname><given-names>A. L.</given-names></name><name><surname>Belfort</surname><given-names>R.</given-names><suffix>Jr.</suffix></name></person-group> (<year>2016a</year>). <article-title>Zika virus in Brazil and macular atrophy in a child with microcephaly.</article-title><source><italic>Lancet</italic></source><volume>387</volume>:<issue>228</issue><pub-id pub-id-type="doi">10.1016/S0140-6736(16)00006-4</pub-id></mixed-citation></ref><ref id="B145"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ventura</surname><given-names>C. V.</given-names></name><name><surname>Maia</surname><given-names>M.</given-names></name><name><surname>Ventura</surname><given-names>B. V.</given-names></name><name><surname>Linden</surname><given-names>V. V.</given-names></name><name><surname>Araujo</surname><given-names>E. B.</given-names></name><name><surname>Ramos</surname><given-names>R. C.</given-names></name><etal></etal></person-group> (<year>2016b</year>). <article-title>Ophthalmological findings in infants with microcephaly and presumable intra-uterus Zika virus infection.</article-title><source><italic>Arq. Bras. Oftalmol.</italic></source><volume>79</volume><fpage>1</fpage>–<lpage>3</lpage>. <pub-id pub-id-type="doi">10.5935/0004-2749.20160002</pub-id><pub-id pub-id-type="pmid">26840156</pub-id></mixed-citation></ref><ref id="B146"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Vorndam</surname><given-names>V.</given-names></name><name><surname>Mathews</surname><given-names>J. H.</given-names></name><name><surname>Barrett</surname><given-names>A. D.</given-names></name><name><surname>Roehrig</surname><given-names>J. T.</given-names></name><name><surname>Trent</surname><given-names>D. W.</given-names></name></person-group> (<year>1993</year>). <article-title>Molecular and biological characterization of a non-glycosylated isolate of St Louis encephalitis virus.</article-title><source><italic>J. Gen. Virol.</italic></source><volume>74(Pt 12)</volume><fpage>2653</fpage>–<lpage>2660</lpage>. <pub-id pub-id-type="doi">10.1099/0022-1317-74-12-2653</pub-id><pub-id pub-id-type="pmid">7506301</pub-id></mixed-citation></ref><ref id="B147"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Waehre</surname><given-names>T.</given-names></name><name><surname>Maagard</surname><given-names>A.</given-names></name><name><surname>Tappe</surname><given-names>D.</given-names></name><name><surname>Cadar</surname><given-names>D.</given-names></name><name><surname>Schmidt-Chanasit</surname><given-names>J.</given-names></name></person-group> (<year>2014</year>). <article-title>Zika virus infection after travel to Tahiti, December 2013.</article-title><source><italic>Emerg. Infect. Dis.</italic></source><volume>20</volume><fpage>1412</fpage>–<lpage>1414</lpage>. <pub-id pub-id-type="doi">10.3201/eid2008.140302</pub-id><pub-id pub-id-type="pmid">25062427</pub-id></mixed-citation></ref><ref id="B148"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Way</surname><given-names>J. H.</given-names></name><name><surname>Bowen</surname><given-names>E. T.</given-names></name><name><surname>Platt</surname><given-names>G. S.</given-names></name></person-group> (<year>1976</year>). <article-title>Comparative studies of some African arboviruses in cell culture and in mice.</article-title><source><italic>J. Gen. Virol.</italic></source><volume>30</volume><fpage>123</fpage>–<lpage>130</lpage>. <pub-id pub-id-type="doi">10.1099/0022-1317-30-1-123</pub-id><pub-id pub-id-type="pmid">1245842</pub-id></mixed-citation></ref><ref id="B149"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Weinbren</surname><given-names>M. P.</given-names></name><name><surname>Williams</surname><given-names>M. C.</given-names></name></person-group> (<year>1958</year>). <article-title>Zika virus: further isolations in the Zika area, and some studies on the strains isolated.</article-title><source><italic>Trans. R. Soc. Trop. Med. Hyg.</italic></source><volume>52</volume><fpage>263</fpage>–<lpage>268</lpage>. <pub-id pub-id-type="doi">10.1016/0035-9203(58)90085-3</pub-id><pub-id pub-id-type="pmid">13556872</pub-id></mixed-citation></ref><ref id="B150"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Weissenbock</surname><given-names>H.</given-names></name><name><surname>Hubalek</surname><given-names>Z.</given-names></name><name><surname>Bakonyi</surname><given-names>T.</given-names></name><name><surname>Nowotny</surname><given-names>N.</given-names></name></person-group> (<year>2010</year>). <article-title>Zoonotic mosquito-borne flaviviruses: worldwide presence of agents with proven pathogenicity and potential candidates of future emerging diseases.</article-title><source><italic>Vet. Microbiol.</italic></source><volume>140</volume><fpage>271</fpage>–<lpage>280</lpage>. <pub-id pub-id-type="doi">10.1016/j.vetmic.2009.08.025</pub-id><pub-id pub-id-type="pmid">19762169</pub-id></mixed-citation></ref><ref id="B151"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Welsch</surname><given-names>S.</given-names></name><name><surname>Miller</surname><given-names>S.</given-names></name><name><surname>Romero-Brey</surname><given-names>I.</given-names></name><name><surname>Merz</surname><given-names>A.</given-names></name><name><surname>Bleck</surname><given-names>C. K.</given-names></name><name><surname>Walther</surname><given-names>P.</given-names></name><etal></etal></person-group> (<year>2009</year>). <article-title>Composition and three-dimensional architecture of the dengue virus replication and assembly sites.</article-title><source><italic>Cell Host Microbe</italic></source><volume>5</volume><fpage>365</fpage>–<lpage>375</lpage>. <pub-id pub-id-type="doi">10.1016/j.chom.2009.03.007</pub-id><pub-id pub-id-type="pmid">19380115</pub-id></mixed-citation></ref><ref id="B152"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Winkler</surname><given-names>G.</given-names></name><name><surname>Heinz</surname><given-names>F. X.</given-names></name><name><surname>Kunz</surname><given-names>C.</given-names></name></person-group> (<year>1987</year>). <article-title>Studies on the glycosylation of flavivirus E proteins and the role of carbohydrate in antigenic structure.</article-title><source><italic>Virology</italic></source><volume>159</volume><fpage>237</fpage>–<lpage>243</lpage>. <pub-id pub-id-type="doi">10.1016/0042-6822(87)90460-0</pub-id><pub-id pub-id-type="pmid">2441520</pub-id></mixed-citation></ref><ref id="B153"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wolfe</surname><given-names>N. D.</given-names></name><name><surname>Kilbourn</surname><given-names>A. M.</given-names></name><name><surname>Karesh</surname><given-names>W. B.</given-names></name><name><surname>Rahman</surname><given-names>H. A.</given-names></name><name><surname>Bosi</surname><given-names>E. J.</given-names></name><name><surname>Cropp</surname><given-names>B. C.</given-names></name><etal></etal></person-group> (<year>2001</year>). <article-title>Sylvatic transmission of arboviruses among Bornean orangutans.</article-title><source><italic>Am. J. Trop. Med. Hyg.</italic></source><volume>64</volume><fpage>310</fpage>–<lpage>316</lpage>.<pub-id pub-id-type="pmid">11463123</pub-id></mixed-citation></ref><ref id="B154"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wong</surname><given-names>P. S.</given-names></name><name><surname>Li</surname><given-names>M. Z.</given-names></name><name><surname>Chong</surname><given-names>C. S.</given-names></name><name><surname>Ng</surname><given-names>L. C.</given-names></name><name><surname>Tan</surname><given-names>C. H.</given-names></name></person-group> (<year>2013</year>). <article-title><italic>Aedes</italic> (<italic>Stegomyia</italic>) albopictus (Skuse): a potential vector of Zika virus in Singapore.</article-title><source><italic>PLoS Negl. Trop. Dis.</italic></source><volume>7</volume>:<issue>e2348</issue><pub-id pub-id-type="doi">10.1371/journal.pntd.0002348PNTD-D-13-00504</pub-id></mixed-citation></ref><ref id="B155"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zammarchi</surname><given-names>L.</given-names></name><name><surname>Stella</surname><given-names>G.</given-names></name><name><surname>Mantella</surname><given-names>A.</given-names></name><name><surname>Bartolozzi</surname><given-names>D.</given-names></name><name><surname>Tappe</surname><given-names>D.</given-names></name><name><surname>Gunther</surname><given-names>S.</given-names></name><etal></etal></person-group> (<year>2015</year>). <article-title>Zika virus infections imported to Italy: clinical, immunological and virological findings, and public health implications.</article-title><source><italic>J. Clin. Virol.</italic></source><volume>63</volume><fpage>32</fpage>–<lpage>35</lpage>. <pub-id pub-id-type="doi">10.1016/j.jcv.2014.12.005</pub-id><pub-id pub-id-type="pmid">25600600</pub-id></mixed-citation></ref><ref id="B156"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zanluca</surname><given-names>C.</given-names></name><name><surname>de Melo</surname><given-names>V. C.</given-names></name><name><surname>Mosimann</surname><given-names>A. L.</given-names></name><name><surname>Dos Santos</surname><given-names>G. I.</given-names></name><name><surname>Dos Santos</surname><given-names>C. N.</given-names></name><name><surname>Luz</surname><given-names>K.</given-names></name></person-group> (<year>2015</year>). <article-title>First report of autochthonous transmission of Zika virus in Brazil.</article-title><source><italic>Mem. Inst. Oswaldo Cruz</italic></source><volume>110</volume><fpage>569</fpage>–<lpage>572</lpage>. <pub-id pub-id-type="doi">10.1590/0074-02760150192</pub-id><pub-id pub-id-type="pmid">26061233</pub-id></mixed-citation></ref></ref-list></back></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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