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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">External Quality Assessment of MERS-CoV Molecular Diagnostics During the 2015 Korean Outbreak</title><author><name sortKey="Seong, Moon Woo" sort="Seong, Moon Woo" uniqKey="Seong M" first="Moon-Woo" last="Seong">Moon-Woo Seong</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Lee, Seung Jun" sort="Lee, Seung Jun" uniqKey="Lee S" first="Seung Jun" last="Lee">Seung Jun Lee</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Cho, Sung Im" sort="Cho, Sung Im" uniqKey="Cho S" first="Sung Im" last="Cho">Sung Im Cho</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Ko, Kyungphil" sort="Ko, Kyungphil" uniqKey="Ko K" first="Kyungphil" last="Ko">Kyungphil Ko</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Kim, Mi Na" sort="Kim, Mi Na" uniqKey="Kim M" first="Mi-Na" last="Kim">Mi-Na Kim</name><affiliation><nlm:aff id="A2">Department of Laboratory Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Sung, Heungsub" sort="Sung, Heungsub" uniqKey="Sung H" first="Heungsub" last="Sung">Heungsub Sung</name><affiliation><nlm:aff id="A2">Department of Laboratory Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Kim, Jae Seok" sort="Kim, Jae Seok" uniqKey="Kim J" first="Jae-Seok" last="Kim">Jae-Seok Kim</name><affiliation><nlm:aff id="A3">Department of Laboratory Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Ahn, Ji Soo" sort="Ahn, Ji Soo" uniqKey="Ahn J" first="Ji Soo" last="Ahn">Ji Soo Ahn</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Yu, Byung Su" sort="Yu, Byung Su" uniqKey="Yu B" first="Byung Su" last="Yu">Byung Su Yu</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Kim, Taek Soo" sort="Kim, Taek Soo" uniqKey="Kim T" first="Taek Soo" last="Kim">Taek Soo Kim</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Kim, Eui Chong" sort="Kim, Eui Chong" uniqKey="Kim E" first="Eui Chong" last="Kim">Eui Chong Kim</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Park, Sung Sup" sort="Park, Sung Sup" uniqKey="Park S" first="Sung Sup" last="Park">Sung Sup Park</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26915611</idno><idno type="pmc">4773263</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773263</idno><idno type="RBID">PMC:4773263</idno><idno type="doi">10.3343/alm.2016.36.3.230</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000079</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000079</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">External Quality Assessment of MERS-CoV Molecular Diagnostics During the 2015 Korean Outbreak</title><author><name sortKey="Seong, Moon Woo" sort="Seong, Moon Woo" uniqKey="Seong M" first="Moon-Woo" last="Seong">Moon-Woo Seong</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Lee, Seung Jun" sort="Lee, Seung Jun" uniqKey="Lee S" first="Seung Jun" last="Lee">Seung Jun Lee</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Cho, Sung Im" sort="Cho, Sung Im" uniqKey="Cho S" first="Sung Im" last="Cho">Sung Im Cho</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Ko, Kyungphil" sort="Ko, Kyungphil" uniqKey="Ko K" first="Kyungphil" last="Ko">Kyungphil Ko</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Kim, Mi Na" sort="Kim, Mi Na" uniqKey="Kim M" first="Mi-Na" last="Kim">Mi-Na Kim</name><affiliation><nlm:aff id="A2">Department of Laboratory Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Sung, Heungsub" sort="Sung, Heungsub" uniqKey="Sung H" first="Heungsub" last="Sung">Heungsub Sung</name><affiliation><nlm:aff id="A2">Department of Laboratory Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Kim, Jae Seok" sort="Kim, Jae Seok" uniqKey="Kim J" first="Jae-Seok" last="Kim">Jae-Seok Kim</name><affiliation><nlm:aff id="A3">Department of Laboratory Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Ahn, Ji Soo" sort="Ahn, Ji Soo" uniqKey="Ahn J" first="Ji Soo" last="Ahn">Ji Soo Ahn</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Yu, Byung Su" sort="Yu, Byung Su" uniqKey="Yu B" first="Byung Su" last="Yu">Byung Su Yu</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Kim, Taek Soo" sort="Kim, Taek Soo" uniqKey="Kim T" first="Taek Soo" last="Kim">Taek Soo Kim</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Kim, Eui Chong" sort="Kim, Eui Chong" uniqKey="Kim E" first="Eui Chong" last="Kim">Eui Chong Kim</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author><author><name sortKey="Park, Sung Sup" sort="Park, Sung Sup" uniqKey="Park S" first="Sung Sup" last="Park">Sung Sup Park</name><affiliation><nlm:aff id="A1">Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</nlm:aff></affiliation></author></analytic><series><title level="j">Annals of Laboratory Medicine</title><idno type="ISSN">2234-3806</idno><idno type="eISSN">2234-3814</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>The largest outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) infection outside Middle East Asia in 2015 has necessitated the rapid expansion of laboratories that conduct MERS-CoV molecular testing in Korea, together with external quality assessment (EQA) to evaluate the assays used.</p></sec><sec><title>Methods</title><p>The EQA program consisted of two phases; self-validation and blind assessment. For the first EQA phase, <italic>in vitro</italic> transcribed upstream region of the envelope gene (upE) and the open reading frame (ORF)1a RNAs were used at a concentration of 1,000 copies/µL. The test panel for the second EQA phase consisted of RNA extracts from three samples, which were obtained from two MERS-CoV positive patients and one MERS-CoV negative patient.</p></sec><sec><title>Results</title><p>The first EQA phase results for 46 participants showed a linear relationship between the threshold cycle (C<sub>T</sub>) values of RNA materials and the logarithmic concentrations for both upE and ORF1a gene targets (R<sup>2</sup>=0.73 and 0.75, respectively). The mean C<sub>T</sub> value for each concentration was different depending on which commercial kit was used for the assay. Among the three commonly used kits, PowerChek MERS Real-Time PCR kit (KogeneBiotech, Korea) showed the lowest C<sub>T</sub> values at all concentrations of upE and most concentrations of ORF1a. The second EQA phase results for 47 participants were 100% correct for all tested samples.</p></sec><sec><title>Conclusions</title><p>This EQA survey demonstrates that the MERS-CoV molecular testing performed in Korea during the 2015 outbreak is of robust capability. However, careful establishment and validation of a cut-off value are recommended to ensure good analytical sensitivity.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="De Graaf, M" uniqKey="De Graaf M">M de Graaf</name></author><author><name sortKey="Lauber, C" uniqKey="Lauber C">C Lauber</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Raj, Vs" uniqKey="Raj V">VS Raj</name></author><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Eckerle, I" uniqKey="Eckerle I">I Eckerle</name></author><author><name sortKey="Bleicker, T" uniqKey="Bleicker T">T Bleicker</name></author><author><name sortKey="Zaki, A" uniqKey="Zaki A">A Zaki</name></author><author><name sortKey="Landt, O" uniqKey="Landt O">O Landt</name></author><author><name sortKey="Eschbach Bludau, M" uniqKey="Eschbach Bludau M">M Eschbach-Bludau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Muller, Ma" uniqKey="Muller M">MA Muller</name></author><author><name sortKey="Costabel, U" uniqKey="Costabel U">U Costabel</name></author><author><name sortKey="Timm, J" uniqKey="Timm J">J Timm</name></author><author><name sortKey="Binger, T" uniqKey="Binger T">T Binger</name></author><author><name sortKey="Meyer, B" uniqKey="Meyer B">B Meyer</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Pas, Sd" uniqKey="Pas S">SD Pas</name></author><author><name sortKey="Patel, P" uniqKey="Patel P">P Patel</name></author><author><name sortKey="Reusken, C" uniqKey="Reusken C">C Reusken</name></author><author><name sortKey="Domingo, C" uniqKey="Domingo C">C Domingo</name></author><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Ann Lab Med</journal-id><journal-id journal-id-type="iso-abbrev">Ann Lab Med</journal-id><journal-id journal-id-type="publisher-id">ALM</journal-id><journal-title-group><journal-title>Annals of Laboratory Medicine</journal-title></journal-title-group><issn pub-type="ppub">2234-3806</issn><issn pub-type="epub">2234-3814</issn><publisher><publisher-name>The Korean Society for Laboratory Medicine</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26915611</article-id><article-id pub-id-type="pmc">4773263</article-id><article-id pub-id-type="doi">10.3343/alm.2016.36.3.230</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject><subj-group subj-group-type="subheading"><subject>Clinical Microbiology</subject></subj-group></subj-group></article-categories><title-group><article-title>External Quality Assessment of MERS-CoV Molecular Diagnostics During the 2015 Korean Outbreak</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Seong</surname><given-names>Moon-Woo</given-names></name><degrees>M.D.</degrees><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Lee</surname><given-names>Seung Jun</given-names></name><degrees>M.D.</degrees><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Cho</surname><given-names>Sung Im</given-names></name><degrees>M.S.</degrees><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Ko</surname><given-names>Kyungphil</given-names></name><degrees>M.T.</degrees><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Mi-Na</given-names></name><degrees>M.D.</degrees><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Sung</surname><given-names>Heungsub</given-names></name><degrees>M.D.</degrees><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Jae-Seok</given-names></name><degrees>M.D.</degrees><xref ref-type="aff" rid="A3">3</xref></contrib><contrib contrib-type="author"><name><surname>Ahn</surname><given-names>Ji Soo</given-names></name><degrees>M.T.</degrees><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Yu</surname><given-names>Byung Su</given-names></name><degrees>M.T.</degrees><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Taek Soo</given-names></name><degrees>M.D.</degrees><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Eui Chong</given-names></name><degrees>M.D.</degrees><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Park</surname><given-names>Sung Sup</given-names></name><degrees>M.D.</degrees><xref ref-type="aff" rid="A1">1</xref></contrib></contrib-group><aff id="A1"><label>1</label>Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</aff><aff id="A2"><label>2</label>Department of Laboratory Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.</aff><aff id="A3"><label>3</label>Department of Laboratory Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea.</aff><author-notes><corresp>Corresponding author: Sung Sup Park. Department of Laboratory Medicine, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea. Tel: +82-2-2072-3206, Fax: +82-2-747-0359, <email>sparkle@snu.ac.kr</email></corresp></author-notes><pub-date pub-type="ppub"><month>5</month><year>2016</year></pub-date><pub-date pub-type="epub"><day>23</day><month>2</month><year>2015</year></pub-date><volume>36</volume><issue>3</issue><fpage>230</fpage><lpage>234</lpage><history><date date-type="received"><day>04</day><month>9</month><year>2015</year></date><date date-type="rev-recd"><day>11</day><month>11</month><year>2015</year></date><date date-type="accepted"><day>29</day><month>1</month><year>2016</year></date></history><permissions><copyright-statement>© The Korean Society for Laboratory Medicine.</copyright-statement><copyright-year>2016</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">http://creativecommons.org/licenses/by-nc/3.0/</ext-link>) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><sec><title>Background</title><p>The largest outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) infection outside Middle East Asia in 2015 has necessitated the rapid expansion of laboratories that conduct MERS-CoV molecular testing in Korea, together with external quality assessment (EQA) to evaluate the assays used.</p></sec><sec><title>Methods</title><p>The EQA program consisted of two phases; self-validation and blind assessment. For the first EQA phase, <italic>in vitro</italic> transcribed upstream region of the envelope gene (upE) and the open reading frame (ORF)1a RNAs were used at a concentration of 1,000 copies/µL. The test panel for the second EQA phase consisted of RNA extracts from three samples, which were obtained from two MERS-CoV positive patients and one MERS-CoV negative patient.</p></sec><sec><title>Results</title><p>The first EQA phase results for 46 participants showed a linear relationship between the threshold cycle (C<sub>T</sub>) values of RNA materials and the logarithmic concentrations for both upE and ORF1a gene targets (R<sup>2</sup>=0.73 and 0.75, respectively). The mean C<sub>T</sub> value for each concentration was different depending on which commercial kit was used for the assay. Among the three commonly used kits, PowerChek MERS Real-Time PCR kit (KogeneBiotech, Korea) showed the lowest C<sub>T</sub> values at all concentrations of upE and most concentrations of ORF1a. The second EQA phase results for 47 participants were 100% correct for all tested samples.</p></sec><sec><title>Conclusions</title><p>This EQA survey demonstrates that the MERS-CoV molecular testing performed in Korea during the 2015 outbreak is of robust capability. However, careful establishment and validation of a cut-off value are recommended to ensure good analytical sensitivity.</p></sec></abstract><kwd-group><kwd>External quality assessment</kwd><kwd>MERS-CoV</kwd><kwd>Molecular diagnostics</kwd><kwd>upE</kwd><kwd>ORF1a</kwd><kwd>Real-time PCR</kwd></kwd-group><funding-group><award-group><funding-source country="KR"><institution-wrap><institution>Korea Centers of Disease Control and Prevention</institution></institution-wrap></funding-source><award-id>2015-P48001-00</award-id></award-group></funding-group></article-meta></front><body><sec sec-type="intro"><title>INTRODUCTION</title><p>Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel human pathogen associated with severe respiratory illness and was first detected in Saudi Arabia in 2012 [<xref rid="B1" ref-type="bibr">1</xref><xref rid="B2" ref-type="bibr">2</xref>]. The confirmatory MERS-CoV test recommended by WHO is based on the real-time reverse transcriptase-PCR (rRT-PCR) of two different targets—an upstream region of the envelope gene (upE; the first-line screening) and a region within the open reading frame (ORF)1a—plus sequencing of the nucleocapsid (N) gene or the RNA-dependent RNA polymerase (RdRP) gene for confirmation [<xref rid="B3" ref-type="bibr">3</xref><xref rid="B4" ref-type="bibr">4</xref><xref rid="B5" ref-type="bibr">5</xref>].</p><p>The first case of MERS-CoV infection in Korea was identified recently, in a 68-yr-old man who had travelled to the Middle East two weeks prior to his diagnosis [<xref rid="B6" ref-type="bibr">6</xref>]. Subsequently, 185 Koreans were infected during a four-week period through contact in hospital or in the household environment [<xref rid="B7" ref-type="bibr">7</xref>]. This was the largest outbreak of MERS-CoV infections outside Middle East Asia, and necessitated the rapid expansion of laboratories to conduct MERS-CoV testing in a short period, as well as the use of assays that had not been approved for diagnostic purposes. Therefore, an urgent external quality assessment (EQA) was proposed in order to assess the quality of the assays used.</p><p>Here, we report our experience in EQA carried out during this MERS-CoV outbreak.</p></sec><sec sec-type="methods"><title>METHODS</title><p>Owing to the rapid introduction of molecular diagnostics for MERS-CoV, the EQA program consisted of two phases. In the first phase, the participants performed a self-validation of their methods using known concentrations of distributed viral material; in the second phase, the participants performed a blind assessment using unknown concentrations of the distributed viral material.</p><sec><title>1. Participants</title><p>Information regarding the MERS-CoV EQA was sent to all institutional members of the Korean Society of Laboratory Medicine. Applications were received via the Korean Society of Laboratory Medicine and the Korean Hospital Association. In total, the number of laboratories that participated in the two phases of this EQA from June 12 2015 to July 10 2015 was 51 and 53, respectively.</p></sec><sec><title>2. Specimen preparation</title><p>For the first EQA phase, <italic>in vitro</italic> transcribed upE and ORF1a RNAs, provided by the University of Bonn Medical Center, Germany, were used at a concentration of 1,000 copies/µL. The test panel for the second EQA phase consisted of RNA extracts from three samples (Sample A, B, and C), two from MERS-CoV-positive patients (representing high positive and low positive levels), and one from a patient who was MERS-CoV-negative. All materials were assayed by using a rRT-PCR test for MERS-CoV RNA as previously described [<xref rid="B3" ref-type="bibr">3</xref><xref rid="B4" ref-type="bibr">4</xref>]. Briefly, a 25-µL reaction was prepared with 5 µL of RNA, 1 µL of each primer, 0.5 µL of probe, 1 µL of enzyme mix, and 12.5 µL of 2× RT-PCR buffer. Reverse transcription was done at 55℃ for 20 min, and PCR cycling conditions were 94℃ for 3 min followed by 45 cycles of 94℃ for 15 sec, 58℃ for 30 sec. The EQA samples were shipped frozen on dry ice by a specialist courier. The following information was collected from the participants: assay kit and platform used, cut-off C<sub>T</sub> value, and the results for each material.</p></sec><sec><title>3. Statistical analysis</title><p>Comparisons of threshold cycle (C<sub>T</sub>) values between assay kits or platforms were performed by using the Mann-Whitney test using MedCalc software package v12.2.1.0 (MedCalc Software, Ostend, Belgium). <italic>P</italic> values <0.05 were considered statistically significant.</p></sec></sec><sec sec-type="results"><title>RESULTS</title><p>The first EQA materials were distributed to 51 laboratories and 46 results were returned (78.4% response rate), and the second EQA materials were distributed to 53 laboratories and 47 results were returned (88.6% response rate). Most of the participants used commercial rRT-PCR kits; of these, PowerChek MERS Real-Time PCR kit (KogeneBiotech, Daejon, Korea) was the most common, followed by DiaPlexQ MERS Virus Detection kit (SolGent, Seoul, Korea), and AccuPower MERS-CoV Real-Time RT-PCR kit (Bioneer, Daejon, Korea) (<xref ref-type="table" rid="T1">Tables 1</xref> and <xref ref-type="table" rid="T2">2</xref>). Only two participants in the first phase and one participant in the second phase of EQA used in-house rRT-PCR methods. All participants reported both upE and ORF1a results in a semi-quantitative manner (C<sub>T</sub> value). Different cut-off values were employed, depending on the kit used: 39, 35, and 38 for AccuPower, PowerChek, and DiaPlexQ, respectively.</p><sec><title>1. The first EQA phase</title><p>In the first phase of EQA, the participants performed a self-validation of their own protocols. The 46 participating laboratories used various concentrations, such as 1,000, 100, 10, and 1 copies/µL. Of these, we excluded validation results at 2×10<sup>2</sup> copies/µL for convenience of interpretation, and thus used only 43 self-validation results in the final analysis.</p><p>The results of first phase EQA are summarized in <xref ref-type="table" rid="T1">Table 1</xref>. The mean C<sub>T</sub> value for each concentration was different, depending on the commercial kit. Among the three commonly used kits, PowerChek showed the lowest C<sub>T</sub> values for all concentrations of upE and for most concentrations of ORF1a. The difference in C<sub>T</sub> values between PowerChek and non-PowerChek kits was significant for upE at concentrations ranging from 1,000 to 10 copies/µL (<italic>P</italic>=0.0003, 0.0002, and 0.0393, respectively). However, in the case of ORF1a, it was significant only at 1,000 and 100 copies/µL but not at 10 copies/µL (<italic>P</italic>=0.0007, 0.0263, and 1.0000, respectively).</p><p>The C<sub>T</sub> values of RNAs had a linear relationship with the logarithmic concentrations for both upE and ORF1a in a boxplot (<xref ref-type="fig" rid="F1">Fig. 1</xref>) (R<sup>2</sup>=0.73 and 0.75, respectively). A linear relationship was also observed for the two common commercial kits, PowerChek and DiaPlexQ: R<sup>2</sup>=0.83 and 0.91 for upE; R<sup>2</sup>=0.86 and 0.71 for ORF1a, respectively.</p><p>Among the results obtained using the PowerChek kit, C<sub>T</sub> values from the ABI 7500 system (Applied Biosystems, Forster City, CA, USA) showed a tendency to be lower than those from BioRad CFX96 (Bio-Rad, Carlsbad, CA, USA), across concentrations, but were not statistically significant (<xref ref-type="table" rid="T3">Table 3</xref>). In addition, the C<sub>T</sub> values of upE were mostly lower than those of ORF1a, but these were also not statistically significant (<xref ref-type="table" rid="T3">Table 3</xref>).</p></sec><sec><title>2. The second EQA phase</title><p>In the second phase EQA, panel results for 47 participants were 100% correct for all three samples tested (<xref ref-type="table" rid="T2">Table 2</xref>). The difference in mean C<sub>T</sub> values between commercial kits in the second EQA phase was similar to that for the first EQA phase. The PowerChek kit showed the lowest C<sub>T</sub> values for upE and ORF1a, and these differences were statistically significant except for ORF1a in sample A (Sample A: <italic>P</italic><0.0001 and <italic>P</italic>=0.0527 for upE and ORF1a, respectively; sample B: <italic>P</italic><0.0001 and <italic>P</italic>=0.0486 for upE and ORF1a, respectively).</p><p>We analyzed the effect of real-time platform on the study results. Among the results obtained using the PowerChek kit, C<sub>T</sub> values from the ABI 7500 system were found to be lower than those from BioRad CFX96 in the two MERS-CoV-positive samples (<xref ref-type="table" rid="T3">Table 3</xref>). These differences were statistically significant except for ORF1a in sample B.</p><p>The C<sub>T</sub> values of upE were also found to be lower than those of ORF1a in the two MERS-CoV-positive samples in the second EQA phase, but were not statistically significant (<italic>P</italic>=0.1742 and 0.1552, respectively).</p></sec></sec><sec sec-type="discussion"><title>DISCUSSION</title><p>Recently, an international EQA of molecular diagnostics for MERS-CoV was conducted across 106 laboratories from six continents; this is the only published report on EQA of MERS-CoV testing [<xref rid="B8" ref-type="bibr">8</xref>]. To the best of our knowledge, this study is the first EQA of MERS-CoV testing conducted in an affected region during an outbreak worldwide. The Korean outbreak of 2015 was the largest outbreak of MERS-CoV infection outside Middle East Asia, which demanded a rapid expansion of MERS-CoV testing laboratories. Unapproved commercial kits for <italic>in vitro</italic> diagnostic use were introduced to clinical laboratories without formal validation as a diagnostic test, which raised an issue of their quality. In this study, more than 46 laboratories participated in EQA over two phases and showed overall good results. Nevertheless, our EQA scheme has some limitations. First, this scheme could not reflect important practical steps, such as sample preparation or nucleic acid extraction, because both <italic>in vitro</italic> transcribed RNA and extracted RNA were used for EQA. Second, for the first phase, the analytical validation was set by using the participants' own dilution scheme and there was no limitation on repeating the tests to obtain the final results. Therefore, not all laboratories provided results for all of the concentrations analyzed in this study, and some positive results for the lower concentrations could be from various test repeats. This means that the results from this EQA phase may be not suitable for inter-laboratory comparison. Third, the second phase assessment comprised only three samples, including one each of strong positive and weak positive, but did not include diverse concentrations especially on the lower end of the analytical sensitivity of rRT-PCR. Lastly, other coronaviruses, such as HCoV-NL63 or HCoV-229E, were not included in the assessment of the analytical specificity of the diagnostic assays.</p><p>In this survey, most participants used commercial rRT-PCR kits. Our results showed that significantly different C<sub>T</sub> values could be produced depending on the kit used. Though these kits are for semi-quantitative purposes and a difference in C<sub>T</sub> values does not correlate with differences in analytical sensitivity, a large difference in the C<sub>T</sub> values for the same material may influence the analytical sensitivity of the kit. Therefore, cut-off values should be carefully validated for each method in order to have optimal sensitivity for the detection of MERS-CoV in clinical settings. In addition, our study demonstrates that C<sub>T</sub> values might be different depending on the platform used for performing real-time PCR, even when using the same commercial kit. Therefore, the platform as well as the kit should be considered when establishing or validating the cut-off value of a kit.</p><p>It is known that the analytical sensitivity of MERS-CoV RT-PCR depends on the target gene tested; the ORF1a assay has a lower sensitivity compared with the upE assay [<xref rid="B3" ref-type="bibr">3</xref>]. In this survey, all participants reported both upE and ORF1a results. Both assays gave comparable results, and no significant difference between them was observed.</p><p>In summary, this EQA survey demonstrates that MERS-CoV molecular testing carried out in Korea during the 2015 outbreak was based on robust methods. However, careful establishment and validation of the cut-off value is recommended to ensure analytical sensitivity.</p></sec></body><back><ack><title>Acknowledgments</title><p>We thank all participating laboratories for their effort and contribution in maintaining good laboratory practice in MERS-CoV testing. We also thank Dr. Christian Drosten from the Institute of Virology, University of Bonn, Germany for providing <italic>in vitro</italic> transcribed RNAs of MERS-CoV genes. 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The central line shows the median and the box covers the interquartile range. The four clusters for the upE gene and ORF1a gene represent 1,000, 100, 10, and 1 copies/µL, respectively. Each cluster denotes results from all methods, PowerChek kit (KogeneBiotech), and DiaPlexQ kit (Solgent), respectively, from left to right.</title></caption><graphic xlink:href="alm-36-230-g001"></graphic></fig><table-wrap id="T1" orientation="portrait" position="float"><label>Table 1</label><caption><title>Summary of results from the first phase of EQA</title></caption><alternatives><graphic xlink:href="alm-36-230-i001"></graphic><table frame="hsides" rules="rows"><thead><tr><th valign="middle" align="left" rowspan="2" colspan="1">Sample (copies/µL)</th><th valign="middle" align="center" rowspan="2" colspan="1">Kit</th><th valign="middle" align="center" rowspan="1" colspan="2">C<sub>T</sub> (Mean±SD)</th><th valign="middle" align="center" rowspan="2" colspan="1">N of participants<sup>*</sup></th></tr><tr><th valign="middle" align="center" rowspan="1" colspan="1">upE</th><th valign="middle" align="center" rowspan="1" colspan="1">ORF1a</th></tr></thead><tbody><tr><td valign="top" align="left" rowspan="5" colspan="1">1,000</td><td valign="top" align="left" rowspan="1" colspan="1">AccuPower</td><td valign="top" align="center" rowspan="1" colspan="1">31.7 ± 1.5</td><td valign="top" align="center" rowspan="1" colspan="1">32.3 ± 0.6</td><td valign="top" align="right" rowspan="1" colspan="1">4</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">PowerChek</td><td valign="top" align="center" rowspan="1" colspan="1">28.6 ± 1.1</td><td valign="top" align="center" rowspan="1" colspan="1">28.6 ± 1.1</td><td valign="top" align="right" rowspan="1" colspan="1">24</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">DiaPlexQ</td><td valign="top" align="center" rowspan="1" colspan="1">29.6 ± 0.5</td><td valign="top" align="center" rowspan="1" colspan="1">30.0 ± 1.2</td><td valign="top" align="right" rowspan="1" colspan="1">5</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Others<sup>†</sup></td><td valign="top" align="center" rowspan="1" colspan="1">30.1 ± 1.0</td><td valign="top" align="center" rowspan="1" colspan="1">29.5 ± 0.9</td><td valign="top" align="right" rowspan="1" colspan="1">4</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Total</td><td valign="top" align="center" rowspan="1" colspan="1">29.3 ± 1.5</td><td valign="top" align="center" rowspan="1" colspan="1">29.3 ± 1.6</td><td valign="top" align="right" rowspan="1" colspan="1">37</td></tr><tr><td valign="top" align="left" rowspan="5" colspan="1">100</td><td valign="top" align="left" rowspan="1" colspan="1">AccuPower</td><td valign="top" align="center" rowspan="1" colspan="1">35.9 ± 1.0</td><td valign="top" align="center" rowspan="1" colspan="1">35.9 ± 1.0</td><td valign="top" align="right" rowspan="1" colspan="1">3</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">PowerChek</td><td valign="top" align="center" rowspan="1" colspan="1">31.8 ± 1.0</td><td valign="top" align="center" rowspan="1" colspan="1">32.2 ± 1.2</td><td valign="top" align="right" rowspan="1" colspan="1">24</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">DiaPlexQ</td><td valign="top" align="center" rowspan="1" colspan="1">33.1 ± 0.9</td><td valign="top" align="center" rowspan="1" colspan="1">32.5 ± 1.1</td><td valign="top" align="right" rowspan="1" colspan="1">5</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Others<sup>‡</sup></td><td valign="top" align="center" rowspan="1" colspan="1">33.9 ± 1.4</td><td valign="top" align="center" rowspan="1" colspan="1">33.5 ± 1.9</td><td valign="top" align="right" rowspan="1" colspan="1">2</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Total</td><td valign="top" align="center" rowspan="1" colspan="1">32.5 ± 1.6</td><td valign="top" align="center" rowspan="1" colspan="1">32.7 ± 1.6</td><td valign="top" align="right" rowspan="1" colspan="1">34</td></tr><tr><td valign="top" align="left" rowspan="5" colspan="1">10</td><td valign="top" align="left" rowspan="1" colspan="1">AccuPower</td><td valign="top" align="center" rowspan="1" colspan="1">NA</td><td valign="top" align="center" rowspan="1" colspan="1">NA</td><td valign="top" align="right" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">PowerChek</td><td valign="top" align="center" rowspan="1" colspan="1">35.3 ± 1.7</td><td valign="top" align="center" rowspan="1" colspan="1">35.9 ± 1.9</td><td valign="top" align="right" rowspan="1" colspan="1">13</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">DiaPlexQ</td><td valign="top" align="center" rowspan="1" colspan="1">38.0 ± 0.0</td><td valign="top" align="center" rowspan="1" colspan="1">35.0 ± 0.0</td><td valign="top" align="right" rowspan="1" colspan="1">1</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Others<sup>‡</sup></td><td valign="top" align="center" rowspan="1" colspan="1">NA</td><td valign="top" align="center" rowspan="1" colspan="1">36.5 ± 2.0</td><td valign="top" align="right" rowspan="1" colspan="1">2</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Total</td><td valign="top" align="center" rowspan="1" colspan="1">35.7 ± 1.8</td><td valign="top" align="center" rowspan="1" colspan="1">36.0 ± 1.8</td><td valign="top" align="right" rowspan="1" colspan="1">16</td></tr><tr><td valign="top" align="left" rowspan="5" colspan="1">1</td><td valign="top" align="left" rowspan="1" colspan="1">AccuPower</td><td valign="top" align="center" rowspan="1" colspan="1">NA</td><td valign="top" align="center" rowspan="1" colspan="1">NA</td><td valign="top" align="right" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">PowerChek</td><td valign="top" align="center" rowspan="1" colspan="1">37.0 ± 1.5</td><td valign="top" align="center" rowspan="1" colspan="1">38.1 ± 0.9</td><td valign="top" align="right" rowspan="1" colspan="1">6</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">DiaPlexQ</td><td valign="top" align="center" rowspan="1" colspan="1">NA</td><td valign="top" align="center" rowspan="1" colspan="1">NA</td><td valign="top" align="right" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Others<sup>‡</sup></td><td valign="top" align="center" rowspan="1" colspan="1">37.8 ± 1.5</td><td valign="top" align="center" rowspan="1" colspan="1">37.5 ± 2.4</td><td valign="top" align="right" rowspan="1" colspan="1">2</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Total</td><td valign="top" align="center" rowspan="1" colspan="1">37.3 ± 1.4</td><td valign="top" align="center" rowspan="1" colspan="1">37.9 ± 1.3</td><td valign="top" align="right" rowspan="1" colspan="1">8</td></tr></tbody></table></alternatives><table-wrap-foot><fn><p><sup>*</sup>The number of participants was different for each concentration because the first EQA phase was based on self-validation; <sup>†</sup>This includes two laboratory-developed tests, MERS-CoV Detection kit (Nanobiosys, Seoul, Korea) and ModularDx kit MERS-CoV (TIB MOLBIOL, Berlin, Germany); <sup>‡</sup>This includes two laboratory-developed tests.</p><p>Abbreviations: EQA, external quality assessment; C<sub>T</sub>, threshold cycle; NA, not applicable.</p></fn></table-wrap-foot></table-wrap><table-wrap id="T2" orientation="portrait" position="float"><label>Table 2</label><caption><title>Summary of results from the second phase of EQA</title></caption><alternatives><graphic xlink:href="alm-36-230-i002"></graphic><table frame="hsides" rules="rows"><thead><tr><th valign="middle" align="left" rowspan="2" colspan="1">Sample</th><th valign="middle" align="center" rowspan="2" colspan="1">Correct results</th><th valign="middle" align="center" rowspan="2" colspan="1">Kit</th><th valign="middle" align="center" rowspan="1" colspan="2">C<sub>T</sub> (Mean±SD)</th><th valign="middle" align="center" rowspan="2" colspan="1">N of participants<sup>*</sup></th></tr><tr><th valign="middle" align="center" rowspan="1" colspan="1">upE</th><th valign="middle" align="center" rowspan="1" colspan="1">ORF1a</th></tr></thead><tbody><tr><td valign="top" align="left" rowspan="5" colspan="1">A</td><td valign="bottom" align="center" rowspan="5" colspan="1">100%</td><td valign="top" align="left" rowspan="1" colspan="1">AccuPower</td><td valign="top" align="center" rowspan="1" colspan="1">28.1 ± 0.6</td><td valign="top" align="center" rowspan="1" colspan="1">29.8 ± 0.2</td><td valign="top" align="right" rowspan="1" colspan="1">4</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">PowerChek</td><td valign="top" align="center" rowspan="1" colspan="1">25.8 ± 0.9</td><td valign="top" align="center" rowspan="1" colspan="1">26.5 ± 0.7</td><td valign="top" align="right" rowspan="1" colspan="1">31</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">DiaPlexQ</td><td valign="top" align="center" rowspan="1" colspan="1">28.0 ± 1.9</td><td valign="top" align="center" rowspan="1" colspan="1">26.8 ± 2.2</td><td valign="top" align="right" rowspan="1" colspan="1">9</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Others<sup>*</sup></td><td valign="top" align="center" rowspan="1" colspan="1">26.5 ± 1.3</td><td valign="top" align="center" rowspan="1" colspan="1">27.1 ± 1.4</td><td valign="top" align="right" rowspan="1" colspan="1">3</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Total</td><td valign="top" align="center" rowspan="1" colspan="1">26.6 ± 1.6</td><td valign="top" align="center" rowspan="1" colspan="1">27.0 ± 1.6</td><td valign="top" align="right" rowspan="1" colspan="1">47</td></tr><tr><td valign="top" align="left" rowspan="5" colspan="1">B</td><td valign="bottom" align="center" rowspan="5" colspan="1">100%</td><td valign="top" align="left" rowspan="1" colspan="1">AccuPower</td><td valign="top" align="center" rowspan="1" colspan="1">31.6 ± 0.7</td><td valign="top" align="center" rowspan="1" colspan="1">33.3 ± 0.2</td><td valign="top" align="right" rowspan="1" colspan="1">4</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">PowerChek</td><td valign="top" align="center" rowspan="1" colspan="1">29.5 ± 1.0</td><td valign="top" align="center" rowspan="1" colspan="1">30.2 ± 0.9</td><td valign="top" align="right" rowspan="1" colspan="1">31</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">DiaPlexQ</td><td valign="top" align="center" rowspan="1" colspan="1">30.5 ± 1.8</td><td valign="top" align="center" rowspan="1" colspan="1">30.6 ± 2.4</td><td valign="top" align="right" rowspan="1" colspan="1">9</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Others<sup>*</sup></td><td valign="top" align="center" rowspan="1" colspan="1">30.1 ± 1.3</td><td valign="top" align="center" rowspan="1" colspan="1">30.7 ± 1.4</td><td valign="top" align="right" rowspan="1" colspan="1">3</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Total</td><td valign="top" align="center" rowspan="1" colspan="1">30.2 ± 1.5</td><td valign="top" align="center" rowspan="1" colspan="1">30.7 ± 1.6</td><td valign="top" align="right" rowspan="1" colspan="1">47</td></tr><tr><td valign="top" align="left" rowspan="5" colspan="1">C</td><td valign="bottom" align="center" rowspan="5" colspan="1">100%</td><td valign="top" align="left" rowspan="1" colspan="1">AccuPower</td><td valign="top" align="center" rowspan="1" colspan="1">Negative</td><td valign="top" align="center" rowspan="1" colspan="1">Negative</td><td valign="top" align="right" rowspan="1" colspan="1">4</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">PowerChek</td><td valign="top" align="center" rowspan="1" colspan="1">Negative</td><td valign="top" align="center" rowspan="1" colspan="1">Negative</td><td valign="top" align="right" rowspan="1" colspan="1">31</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">DiaPlexQ</td><td valign="top" align="center" rowspan="1" colspan="1">Negative</td><td valign="top" align="center" rowspan="1" colspan="1">Negative</td><td valign="top" align="right" rowspan="1" colspan="1">9</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Others<sup>*</sup></td><td valign="top" align="center" rowspan="1" colspan="1">Negative</td><td valign="top" align="center" rowspan="1" colspan="1">Negative</td><td valign="top" align="right" rowspan="1" colspan="1">3</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Total</td><td valign="top" align="center" rowspan="1" colspan="1">Negative</td><td valign="top" align="center" rowspan="1" colspan="1">Negative</td><td valign="top" align="right" rowspan="1" colspan="1">47</td></tr></tbody></table></alternatives><table-wrap-foot><fn><p><sup>*</sup>This includes laboratory-developed test, MERS-CoV Detection kit (Nanobiosys, Seoul, Korea) and ModularDx kit MERS-CoV (TIB MOLBIOL, Berlin, Germany).</p><p>Abbreviations: EQA, external quality assessment; C<sub>T</sub>, threshold cycle.</p></fn></table-wrap-foot></table-wrap><table-wrap id="T3" orientation="portrait" position="float"><label>Table 3</label><caption><title>Comparison of C<sub>T</sub> values between two real-time PCR platforms using PowerChek kit</title></caption><alternatives><graphic xlink:href="alm-36-230-i003"></graphic><table frame="hsides" rules="rows"><thead><tr><th valign="middle" align="left" rowspan="2" colspan="1">EQA phase</th><th valign="middle" align="center" rowspan="2" colspan="1">Materials</th><th valign="middle" align="center" rowspan="2" colspan="1">Target</th><th valign="middle" align="center" rowspan="1" colspan="4">C<sub>T</sub> (Mean±SD)</th><th valign="middle" align="center" rowspan="2" colspan="1"><italic>P</italic> value<sup>*</sup></th></tr><tr><th valign="middle" align="center" rowspan="1" colspan="1">ABI</th><th valign="middle" align="center" rowspan="1" colspan="1">N</th><th valign="middle" align="center" rowspan="1" colspan="1">BioRad</th><th valign="middle" align="center" rowspan="1" colspan="1">N</th></tr></thead><tbody><tr><td valign="top" align="left" rowspan="6" colspan="1">1st</td><td valign="top" align="center" rowspan="2" colspan="1">1,000</td><td valign="top" align="left" rowspan="1" colspan="1">upE</td><td valign="top" align="center" rowspan="1" colspan="1">28.2 ± 1.3</td><td valign="top" align="right" rowspan="1" colspan="1">10</td><td valign="top" align="center" rowspan="1" colspan="1">29.0±0.8</td><td valign="top" align="right" rowspan="1" colspan="1">13</td><td valign="top" align="center" rowspan="1" colspan="1">NS</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">ORF1a</td><td valign="top" align="center" rowspan="1" colspan="1">28.3 ± 1.1</td><td valign="top" align="right" rowspan="1" colspan="1">8</td><td valign="top" align="center" rowspan="1" colspan="1">28.8±1.1</td><td valign="top" align="right" rowspan="1" colspan="1">11</td><td valign="top" align="center" rowspan="1" colspan="1">NS</td></tr><tr><td valign="top" align="center" rowspan="2" colspan="1">100</td><td valign="top" align="left" rowspan="1" colspan="1">upE</td><td valign="top" align="center" rowspan="1" colspan="1">31.5 ± 1.2</td><td valign="top" align="right" rowspan="1" colspan="1">10</td><td valign="top" align="center" rowspan="1" colspan="1">32.0±1.0</td><td valign="top" align="right" rowspan="1" colspan="1">13</td><td valign="top" align="center" rowspan="1" colspan="1">NS</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">ORF1a</td><td valign="top" align="center" rowspan="1" colspan="1">31.7 ± 0.7</td><td valign="top" align="right" rowspan="1" colspan="1">10</td><td valign="top" align="center" rowspan="1" colspan="1">32.4 ±1.4</td><td valign="top" align="right" rowspan="1" colspan="1">12</td><td valign="top" align="center" rowspan="1" colspan="1">NS</td></tr><tr><td valign="top" align="center" rowspan="2" colspan="1">10</td><td valign="top" align="left" rowspan="1" colspan="1">upE</td><td valign="top" align="center" rowspan="1" colspan="1">34.5 ± 1.2</td><td valign="top" align="right" rowspan="1" colspan="1">5</td><td valign="top" align="center" rowspan="1" colspan="1">36.1±1.2</td><td valign="top" align="right" rowspan="1" colspan="1">7</td><td valign="top" align="center" rowspan="1" colspan="1">NS</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">ORF1a</td><td valign="top" align="center" rowspan="1" colspan="1">35.4 ± 1.2</td><td valign="top" align="right" rowspan="1" colspan="1">5</td><td valign="top" align="center" rowspan="1" colspan="1">36.9±2.2</td><td valign="top" align="right" rowspan="1" colspan="1">6</td><td valign="top" align="center" rowspan="1" colspan="1">NS</td></tr><tr><td valign="top" align="left" rowspan="4" colspan="1">2nd</td><td valign="top" align="center" rowspan="2" colspan="1">A</td><td valign="top" align="left" rowspan="1" colspan="1">upE</td><td valign="top" align="center" rowspan="1" colspan="1">25.5 ± 0.7</td><td valign="top" align="right" rowspan="1" colspan="1">14</td><td valign="top" align="center" rowspan="1" colspan="1">26.3±0.6</td><td valign="top" align="right" rowspan="1" colspan="1">15</td><td valign="top" align="center" rowspan="1" colspan="1">0.0011</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">ORF1a</td><td valign="top" align="center" rowspan="1" colspan="1">26.4 ± 0.7</td><td valign="top" align="right" rowspan="1" colspan="1">14</td><td valign="top" align="center" rowspan="1" colspan="1">26.8±0.7</td><td valign="top" align="right" rowspan="1" colspan="1">15</td><td valign="top" align="center" rowspan="1" colspan="1">0.0447</td></tr><tr><td valign="top" align="center" rowspan="2" colspan="1">B</td><td valign="top" align="left" rowspan="1" colspan="1">upE</td><td valign="top" align="center" rowspan="1" colspan="1">29.2 ± 0.7</td><td valign="top" align="right" rowspan="1" colspan="1">14</td><td valign="top" align="center" rowspan="1" colspan="1">30.0±0.7</td><td valign="top" align="right" rowspan="1" colspan="1">15</td><td valign="top" align="center" rowspan="1" colspan="1">0.0035</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">ORF1a</td><td valign="top" align="center" rowspan="1" colspan="1">30.0 ± 0.9</td><td valign="top" align="right" rowspan="1" colspan="1">14</td><td valign="top" align="center" rowspan="1" colspan="1">30.5±0.8</td><td valign="top" align="right" rowspan="1" colspan="1">15</td><td valign="top" align="center" rowspan="1" colspan="1">NS</td></tr></tbody></table></alternatives><table-wrap-foot><fn><p><sup>*</sup>The comparison of C<sub>T</sub> values between the two groups was performed by using the Mann-Whitney test because sample sizes of some groups were small.</p><p>Abbrviations: EQA, external quality assessment; C<sub>T</sub>, threshold cycle; NS, not 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