Degradation of nanoRNA is performed by multiple redundant RNases in Bacillus subtilis
Identifieur interne : 001379 ( Pmc/Checkpoint ); précédent : 001378; suivant : 001380Degradation of nanoRNA is performed by multiple redundant RNases in Bacillus subtilis
Auteurs : Ming Fang ; Wencke-Maria Zeisberg ; Ciaran Condon [France] ; Vasily Ogryzko [France] ; Antoine Danchin ; Undine MecholdSource :
- Nucleic Acids Research [ 0305-1048 ] ; 2009.
Abstract
Url:
DOI: 10.1093/nar/gkp527
PubMed: 19553197
PubMed Central: 2731908
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:2731908Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Degradation of nanoRNA is performed by multiple redundant RNases in <italic>Bacillus subtilis</italic></title><author><name sortKey="Fang, Ming" sort="Fang, Ming" uniqKey="Fang M" first="Ming" last="Fang">Ming Fang</name><affiliation><nlm:aff id="AFF1">Institut Pasteur, URA 2171, Unité de Génétique des Génomes Bactériens, 75724 Paris Cedex 15,</nlm:aff><wicri:noCountry code="subfield">75724 Paris Cedex 15</wicri:noCountry></affiliation></author><author><name sortKey="Zeisberg, Wencke Maria" sort="Zeisberg, Wencke Maria" uniqKey="Zeisberg W" first="Wencke-Maria" last="Zeisberg">Wencke-Maria Zeisberg</name><affiliation><nlm:aff id="AFF1">Institut Pasteur, URA 2171, Unité de Génétique des Génomes Bactériens, 75724 Paris Cedex 15,</nlm:aff><wicri:noCountry code="subfield">75724 Paris Cedex 15</wicri:noCountry></affiliation></author><author><name sortKey="Condon, Ciaran" sort="Condon, Ciaran" uniqKey="Condon C" first="Ciaran" last="Condon">Ciaran Condon</name><affiliation wicri:level="1"><nlm:aff wicri:cut=" and" id="AFF1">Institut de Biologie Physico-Chimique, CNRS UPR 9073, 75005 Paris</nlm:aff><country>France</country><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName><wicri:regionArea>Institut de Biologie Physico-Chimique, CNRS UPR 9073</wicri:regionArea><wicri:noRegion>CNRS UPR 9073</wicri:noRegion><wicri:noRegion>CNRS UPR 9073</wicri:noRegion></affiliation></author><author><name sortKey="Ogryzko, Vasily" sort="Ogryzko, Vasily" uniqKey="Ogryzko V" first="Vasily" last="Ogryzko">Vasily Ogryzko</name><affiliation wicri:level="3"><nlm:aff id="AFF1">Institut Gustave Roussy, UMR 8126, Unité d’ Interactions Moléculaires et Cancer, 94805 Villejuif, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut Gustave Roussy, UMR 8126, Unité d’ Interactions Moléculaires et Cancer, 94805 Villejuif</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Villejuif</settlement></placeName></affiliation></author><author><name sortKey="Danchin, Antoine" sort="Danchin, Antoine" uniqKey="Danchin A" first="Antoine" last="Danchin">Antoine Danchin</name><affiliation><nlm:aff id="AFF1">Institut Pasteur, URA 2171, Unité de Génétique des Génomes Bactériens, 75724 Paris Cedex 15,</nlm:aff><wicri:noCountry code="subfield">75724 Paris Cedex 15</wicri:noCountry></affiliation></author><author><name sortKey="Mechold, Undine" sort="Mechold, Undine" uniqKey="Mechold U" first="Undine" last="Mechold">Undine Mechold</name><affiliation><nlm:aff id="AFF1">Institut Pasteur, URA 2171, Unité de Génétique des Génomes Bactériens, 75724 Paris Cedex 15,</nlm:aff><wicri:noCountry code="subfield">75724 Paris Cedex 15</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19553197</idno><idno type="pmc">2731908</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731908</idno><idno type="RBID">PMC:2731908</idno><idno type="doi">10.1093/nar/gkp527</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">000F43</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000F43</idno><idno type="wicri:Area/Pmc/Curation">000F43</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000F43</idno><idno type="wicri:Area/Pmc/Checkpoint">001379</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001379</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Degradation of nanoRNA is performed by multiple redundant RNases in <italic>Bacillus subtilis</italic></title><author><name sortKey="Fang, Ming" sort="Fang, Ming" uniqKey="Fang M" first="Ming" last="Fang">Ming Fang</name><affiliation><nlm:aff id="AFF1">Institut Pasteur, URA 2171, Unité de Génétique des Génomes Bactériens, 75724 Paris Cedex 15,</nlm:aff><wicri:noCountry code="subfield">75724 Paris Cedex 15</wicri:noCountry></affiliation></author><author><name sortKey="Zeisberg, Wencke Maria" sort="Zeisberg, Wencke Maria" uniqKey="Zeisberg W" first="Wencke-Maria" last="Zeisberg">Wencke-Maria Zeisberg</name><affiliation><nlm:aff id="AFF1">Institut Pasteur, URA 2171, Unité de Génétique des Génomes Bactériens, 75724 Paris Cedex 15,</nlm:aff><wicri:noCountry code="subfield">75724 Paris Cedex 15</wicri:noCountry></affiliation></author><author><name sortKey="Condon, Ciaran" sort="Condon, Ciaran" uniqKey="Condon C" first="Ciaran" last="Condon">Ciaran Condon</name><affiliation wicri:level="1"><nlm:aff wicri:cut=" and" id="AFF1">Institut de Biologie Physico-Chimique, CNRS UPR 9073, 75005 Paris</nlm:aff><country>France</country><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName><wicri:regionArea>Institut de Biologie Physico-Chimique, CNRS UPR 9073</wicri:regionArea><wicri:noRegion>CNRS UPR 9073</wicri:noRegion><wicri:noRegion>CNRS UPR 9073</wicri:noRegion></affiliation></author><author><name sortKey="Ogryzko, Vasily" sort="Ogryzko, Vasily" uniqKey="Ogryzko V" first="Vasily" last="Ogryzko">Vasily Ogryzko</name><affiliation wicri:level="3"><nlm:aff id="AFF1">Institut Gustave Roussy, UMR 8126, Unité d’ Interactions Moléculaires et Cancer, 94805 Villejuif, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut Gustave Roussy, UMR 8126, Unité d’ Interactions Moléculaires et Cancer, 94805 Villejuif</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Villejuif</settlement></placeName></affiliation></author><author><name sortKey="Danchin, Antoine" sort="Danchin, Antoine" uniqKey="Danchin A" first="Antoine" last="Danchin">Antoine Danchin</name><affiliation><nlm:aff id="AFF1">Institut Pasteur, URA 2171, Unité de Génétique des Génomes Bactériens, 75724 Paris Cedex 15,</nlm:aff><wicri:noCountry code="subfield">75724 Paris Cedex 15</wicri:noCountry></affiliation></author><author><name sortKey="Mechold, Undine" sort="Mechold, Undine" uniqKey="Mechold U" first="Undine" last="Mechold">Undine Mechold</name><affiliation><nlm:aff id="AFF1">Institut Pasteur, URA 2171, Unité de Génétique des Génomes Bactériens, 75724 Paris Cedex 15,</nlm:aff><wicri:noCountry code="subfield">75724 Paris Cedex 15</wicri:noCountry></affiliation></author></analytic><series><title level="j">Nucleic Acids Research</title><idno type="ISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><italic>Escherichia coli</italic> possesses only one essential oligoribonuclease (Orn), an enzyme that can degrade oligoribonucleotides of five residues and shorter in length (nanoRNA). Firmicutes including <italic>Bacillus subtilis</italic> do not have an Orn homolog. We had previously identified YtqI (NrnA) as functional analog of Orn in <italic>B. subtilis</italic>. Screening a genomic library from <italic>B. subtilis</italic> for genes that can complement a conditional <italic>orn</italic> mutant, we identify here YngD (NrnB) as a second nanoRNase in <italic>B. subtilis</italic>. Like NrnA, NrnB is a member of the DHH/DHHA1 protein family of phosphoesterases. NrnB degrades nanoRNA 5-mers <italic>in vitro</italic> similarily to Orn. Low expression levels of NrnB are sufficient for <italic>orn</italic> complementation. YhaM, a known RNase present in <italic>B. subtilis</italic>, degrades nanoRNA efficiently <italic>in vitro</italic> but requires high levels of expression for only partial complementation of the <italic>orn<sup>–</sup></italic> strain. A triple mutant (<italic>nrnA</italic><sup>–</sup>, <italic>nrnB</italic><sup>–</sup>, <italic>yhaM</italic><sup>–</sup>) in <italic>B. subtilis</italic> is viable and shows almost no impairment in growth. Lastly, RNase J1 seems also to have some 5′-to-3′ exoribonuclease activity on nanoRNA and thus can potentially finish degradation of RNA. We conclude that, unlike in <italic>E. coli</italic>, degradation of nanoRNA is performed in a redundant fashion in <italic>B. subtilis</italic>.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Condon, C" uniqKey="Condon C">C Condon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheng, Zf" uniqKey="Cheng Z">ZF Cheng</name></author><author><name sortKey="Deutscher, Mp" uniqKey="Deutscher M">MP Deutscher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Amblar, M" uniqKey="Amblar M">M Amblar</name></author><author><name sortKey="Barbas, A" uniqKey="Barbas A">A Barbas</name></author><author><name sortKey="Fialho, Am" uniqKey="Fialho A">AM Fialho</name></author><author><name sortKey="Arraiano, Cm" uniqKey="Arraiano C">CM Arraiano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Amblar, M" uniqKey="Amblar M">M Amblar</name></author><author><name sortKey="Barbas, A" uniqKey="Barbas A">A Barbas</name></author><author><name sortKey="Gomez Puertas, P" uniqKey="Gomez Puertas P">P Gomez-Puertas</name></author><author><name sortKey="Arraiano, Cm" uniqKey="Arraiano C">CM Arraiano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vincent, Ha" uniqKey="Vincent H">HA Vincent</name></author><author><name sortKey="Deutscher, Mp" uniqKey="Deutscher M">MP Deutscher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mechold, U" uniqKey="Mechold U">U Mechold</name></author><author><name sortKey="Fang, G" uniqKey="Fang G">G Fang</name></author><author><name sortKey="Ngo, S" uniqKey="Ngo S">S Ngo</name></author><author><name sortKey="Ogryzko, V" uniqKey="Ogryzko V">V Ogryzko</name></author><author><name sortKey="Danchin, A" uniqKey="Danchin A">A Danchin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ghosh, S" uniqKey="Ghosh S">S Ghosh</name></author><author><name sortKey="Deutscher, Mp" uniqKey="Deutscher M">MP Deutscher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, D" uniqKey="Yu D">D Yu</name></author><author><name sortKey="Deutscher, Mp" uniqKey="Deutscher M">MP Deutscher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, X" uniqKey="Zhang X">X Zhang</name></author><author><name sortKey="Zhu, L" uniqKey="Zhu L">L Zhu</name></author><author><name sortKey="Deutscher, Mp" uniqKey="Deutscher M">MP Deutscher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mathy, N" uniqKey="Mathy N">N Mathy</name></author><author><name sortKey="Benard, L" uniqKey="Benard L">L Benard</name></author><author><name sortKey="Pellegrini, O" uniqKey="Pellegrini O">O Pellegrini</name></author><author><name sortKey="Daou, R" uniqKey="Daou R">R Daou</name></author><author><name sortKey="Wen, T" uniqKey="Wen T">T Wen</name></author><author><name sortKey="Condon, C" uniqKey="Condon C">C Condon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mechold, U" uniqKey="Mechold U">U Mechold</name></author><author><name sortKey="Ogryzko, V" uniqKey="Ogryzko V">V Ogryzko</name></author><author><name sortKey="Ngo, S" uniqKey="Ngo S">S Ngo</name></author><author><name sortKey="Danchin, A" uniqKey="Danchin A">A Danchin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stragier, P" uniqKey="Stragier P">P Stragier</name></author><author><name sortKey="Bonamy, C" uniqKey="Bonamy C">C Bonamy</name></author><author><name sortKey="Karmazyn Campelli, C" uniqKey="Karmazyn Campelli C">C Karmazyn-Campelli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kobayashi, K" uniqKey="Kobayashi K">K Kobayashi</name></author><author><name sortKey="Ehrlich, Sd" uniqKey="Ehrlich S">SD Ehrlich</name></author><author><name sortKey="Albertini, A" uniqKey="Albertini A">A Albertini</name></author><author><name sortKey="Amati, G" uniqKey="Amati G">G Amati</name></author><author><name sortKey="Andersen, Kk" uniqKey="Andersen K">KK Andersen</name></author><author><name sortKey="Arnaud, M" uniqKey="Arnaud M">M Arnaud</name></author><author><name sortKey="Asai, K" uniqKey="Asai K">K Asai</name></author><author><name sortKey="Ashikaga, S" uniqKey="Ashikaga S">S Ashikaga</name></author><author><name sortKey="Aymerich, S" uniqKey="Aymerich S">S Aymerich</name></author><author><name sortKey="Bessieres, P" uniqKey="Bessieres P">P Bessieres</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Oussenko, Ia" uniqKey="Oussenko I">IA Oussenko</name></author><author><name sortKey="Sanchez, R" uniqKey="Sanchez R">R Sanchez</name></author><author><name sortKey="Bechhofer, Dh" uniqKey="Bechhofer D">DH Bechhofer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bechhofer, Dh" uniqKey="Bechhofer D">DH Bechhofer</name></author><author><name sortKey="Oussenko, Ia" uniqKey="Oussenko I">IA Oussenko</name></author><author><name sortKey="Deikus, G" uniqKey="Deikus G">G Deikus</name></author><author><name sortKey="Yao, S" uniqKey="Yao S">S Yao</name></author><author><name sortKey="Mathy, N" uniqKey="Mathy N">N Mathy</name></author><author><name sortKey="Condon, C" uniqKey="Condon C">C Condon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aravind, L" uniqKey="Aravind L">L Aravind</name></author><author><name sortKey="Koonin, Ev" uniqKey="Koonin E">EV Koonin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sutera, V A" uniqKey="Sutera V">V.A. Sutera</name></author><author><name sortKey="Han, Es" uniqKey="Han E">ES Han</name></author><author><name sortKey="Rajman, La" uniqKey="Rajman L">LA Rajman</name></author><author><name sortKey="Lovett, St" uniqKey="Lovett S">ST Lovett</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yamagata, A" uniqKey="Yamagata A">A Yamagata</name></author><author><name sortKey="Kakuta, Y" uniqKey="Kakuta Y">Y Kakuta</name></author><author><name sortKey="Masui, R" uniqKey="Masui R">R Masui</name></author><author><name sortKey="Fukuyama, K" uniqKey="Fukuyama K">K Fukuyama</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tammenkoski, M" uniqKey="Tammenkoski M">M Tammenkoski</name></author><author><name sortKey="Moiseev, Vm" uniqKey="Moiseev V">VM Moiseev</name></author><author><name sortKey="Lahti, M" uniqKey="Lahti M">M Lahti</name></author><author><name sortKey="Ugochukwu, E" uniqKey="Ugochukwu E">E Ugochukwu</name></author><author><name sortKey="Brondijk, Th" uniqKey="Brondijk T">TH Brondijk</name></author><author><name sortKey="White, Sa" uniqKey="White S">SA White</name></author><author><name sortKey="Lahti, R" uniqKey="Lahti R">R Lahti</name></author><author><name sortKey="Baykov, Aa" uniqKey="Baykov A">AA Baykov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="D Angelo, A" uniqKey="D Angelo A">A D'Angelo</name></author><author><name sortKey="Garzia, L" uniqKey="Garzia L">L Garzia</name></author><author><name sortKey="Andre, A" uniqKey="Andre A">A Andre</name></author><author><name sortKey="Carotenuto, P" uniqKey="Carotenuto P">P Carotenuto</name></author><author><name sortKey="Aglio, V" uniqKey="Aglio V">V Aglio</name></author><author><name sortKey="Guardiola, O" uniqKey="Guardiola O">O Guardiola</name></author><author><name sortKey="Arrigoni, G" uniqKey="Arrigoni G">G Arrigoni</name></author><author><name sortKey="Cossu, A" uniqKey="Cossu A">A Cossu</name></author><author><name sortKey="Palmieri, G" uniqKey="Palmieri G">G Palmieri</name></author><author><name sortKey="Aravind, L" uniqKey="Aravind L">L Aravind</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Feucht, A" uniqKey="Feucht A">A Feucht</name></author><author><name sortKey="Evans, L" uniqKey="Evans L">L Evans</name></author><author><name sortKey="Errington, J" uniqKey="Errington J">J Errington</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Oussenko, Ia" uniqKey="Oussenko I">IA Oussenko</name></author><author><name sortKey="Abe, T" uniqKey="Abe T">T Abe</name></author><author><name sortKey="Ujiie, H" uniqKey="Ujiie H">H Ujiie</name></author><author><name sortKey="Muto, A" uniqKey="Muto A">A Muto</name></author><author><name sortKey="Bechhofer, Dh" uniqKey="Bechhofer D">DH Bechhofer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Au, N" uniqKey="Au N">N Au</name></author><author><name sortKey="Kuester Schoeck, E" uniqKey="Kuester Schoeck E">E Kuester-Schoeck</name></author><author><name sortKey="Mandava, V" uniqKey="Mandava V">V Mandava</name></author><author><name sortKey="Bothwell, Le" uniqKey="Bothwell L">LE Bothwell</name></author><author><name sortKey="Canny, Sp" uniqKey="Canny S">SP Canny</name></author><author><name sortKey="Chachu, K" uniqKey="Chachu K">K Chachu</name></author><author><name sortKey="Colavito, Sa" uniqKey="Colavito S">SA Colavito</name></author><author><name sortKey="Fuller, Sn" uniqKey="Fuller S">SN Fuller</name></author><author><name sortKey="Groban, Es" uniqKey="Groban E">ES Groban</name></author><author><name sortKey="Hensley, La" uniqKey="Hensley L">LA Hensley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marchler Bauer, A" uniqKey="Marchler Bauer A">A Marchler-Bauer</name></author><author><name sortKey="Anderson, Jb" uniqKey="Anderson J">JB Anderson</name></author><author><name sortKey="Derbyshire, Mk" uniqKey="Derbyshire M">MK Derbyshire</name></author><author><name sortKey="Deweese Scott, C" uniqKey="Deweese Scott C">C DeWeese-Scott</name></author><author><name sortKey="Gonzales, Nr" uniqKey="Gonzales N">NR Gonzales</name></author><author><name sortKey="Gwadz, M" uniqKey="Gwadz M">M Gwadz</name></author><author><name sortKey="Hao, L" uniqKey="Hao L">L Hao</name></author><author><name sortKey="He, S" uniqKey="He S">S He</name></author><author><name sortKey="Hurwitz, Di" uniqKey="Hurwitz D">DI Hurwitz</name></author><author><name sortKey="Jackson, Jd" uniqKey="Jackson J">JD Jackson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Noirot Gros, Mf" uniqKey="Noirot Gros M">MF Noirot-Gros</name></author><author><name sortKey="Dervyn, E" uniqKey="Dervyn E">E Dervyn</name></author><author><name sortKey="Wu, Lj" uniqKey="Wu L">LJ Wu</name></author><author><name sortKey="Mervelet, P" uniqKey="Mervelet P">P Mervelet</name></author><author><name sortKey="Errington, J" uniqKey="Errington J">J Errington</name></author><author><name sortKey="Ehrlich, Sd" uniqKey="Ehrlich S">SD Ehrlich</name></author><author><name sortKey="Noirot, P" uniqKey="Noirot P">P Noirot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Deikus, G" uniqKey="Deikus G">G Deikus</name></author><author><name sortKey="Condon, C" uniqKey="Condon C">C Condon</name></author><author><name sortKey="Bechhofer, Dh" uniqKey="Bechhofer D">DH Bechhofer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mader, U" uniqKey="Mader U">U Mader</name></author><author><name sortKey="Zig, L" uniqKey="Zig L">L Zig</name></author><author><name sortKey="Kretschmer, J" uniqKey="Kretschmer J">J Kretschmer</name></author><author><name sortKey="Homuth, G" uniqKey="Homuth G">G Homuth</name></author><author><name sortKey="Putzer, H" uniqKey="Putzer H">H Putzer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pushker, R" uniqKey="Pushker R">R Pushker</name></author><author><name sortKey="Mira, A" uniqKey="Mira A">A Mira</name></author><author><name sortKey="Rodriguez Valera, F" uniqKey="Rodriguez Valera F">F Rodriguez-Valera</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, D" uniqKey="Yu D">D Yu</name></author><author><name sortKey="Ellis, Hm" uniqKey="Ellis H">HM Ellis</name></author><author><name sortKey="Lee, Ec" uniqKey="Lee E">EC Lee</name></author><author><name sortKey="Jenkins, Na" uniqKey="Jenkins N">NA Jenkins</name></author><author><name sortKey="Copeland, Ng" uniqKey="Copeland N">NG Copeland</name></author><author><name sortKey="Court, Dl" uniqKey="Court D">DL Court</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guzman, Lm" uniqKey="Guzman L">LM Guzman</name></author><author><name sortKey="Belin, D" uniqKey="Belin D">D Belin</name></author><author><name sortKey="Carson, Mj" uniqKey="Carson M">MJ Carson</name></author><author><name sortKey="Beckwith, J" uniqKey="Beckwith J">J Beckwith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Petit, Ma" uniqKey="Petit M">MA Petit</name></author><author><name sortKey="Dervyn, E" uniqKey="Dervyn E">E Dervyn</name></author><author><name sortKey="Rose, M" uniqKey="Rose M">M Rose</name></author><author><name sortKey="Entian, Kd" uniqKey="Entian K">KD Entian</name></author><author><name sortKey="Mcgovern, S" uniqKey="Mcgovern S">S McGovern</name></author><author><name sortKey="Ehrlich, Sd" uniqKey="Ehrlich S">SD Ehrlich</name></author><author><name sortKey="Bruand, C" uniqKey="Bruand C">C Bruand</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vagner, V" uniqKey="Vagner V">V Vagner</name></author><author><name sortKey="Dervyn, E" uniqKey="Dervyn E">E Dervyn</name></author><author><name sortKey="Ehrlich, Sd" uniqKey="Ehrlich S">SD Ehrlich</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sullivan, Ma" uniqKey="Sullivan M">MA Sullivan</name></author><author><name sortKey="Yasbin, Re" uniqKey="Yasbin R">RE Yasbin</name></author><author><name sortKey="Young, Fe" uniqKey="Young F">FE Young</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Nucleic Acids Res</journal-id><journal-id journal-id-type="iso-abbrev">Nucleic Acids Res</journal-id><journal-id journal-id-type="publisher-id">nar</journal-id><journal-id journal-id-type="hwp">nar</journal-id><journal-title-group><journal-title>Nucleic Acids Research</journal-title></journal-title-group><issn pub-type="ppub">0305-1048</issn><issn pub-type="epub">1362-4962</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">19553197</article-id><article-id pub-id-type="pmc">2731908</article-id><article-id pub-id-type="doi">10.1093/nar/gkp527</article-id><article-id pub-id-type="publisher-id">gkp527</article-id><article-categories><subj-group subj-group-type="heading"><subject>Nucleic Acid Enzymes</subject></subj-group></article-categories><title-group><article-title>Degradation of nanoRNA is performed by multiple redundant RNases in <italic>Bacillus subtilis</italic></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Fang</surname><given-names>Ming</given-names></name><xref ref-type="aff" rid="AFF1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zeisberg</surname><given-names>Wencke-Maria</given-names></name><xref ref-type="aff" rid="AFF1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Condon</surname><given-names>Ciaran</given-names></name><xref ref-type="aff" rid="AFF1"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ogryzko</surname><given-names>Vasily</given-names></name><xref ref-type="aff" rid="AFF1"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Danchin</surname><given-names>Antoine</given-names></name><xref ref-type="aff" rid="AFF1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Mechold</surname><given-names>Undine</given-names></name><xref ref-type="aff" rid="AFF1"><sup>1</sup></xref><xref ref-type="corresp" rid="COR1">*</xref></contrib></contrib-group><aff id="AFF1"><sup>1</sup>Institut Pasteur, URA 2171, Unité de Génétique des Génomes Bactériens, 75724 Paris Cedex 15,<sup>2</sup>Institut de Biologie Physico-Chimique, CNRS UPR 9073, 75005 Paris and<sup>3</sup>Institut Gustave Roussy, UMR 8126, Unité d’ Interactions Moléculaires et Cancer, 94805 Villejuif, France</aff><author-notes><corresp id="COR1">*To whom correspondence should be addressed. Tel: <phone>+33 14 0613870</phone>; Fax: <fax>+33 14 5688948</fax>; Email: <email>undine.mechold@pasteur.fr</email></corresp></author-notes><pub-date pub-type="ppub"><month>8</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>24</day><month>6</month><year>2009</year></pub-date><pub-date pub-type="pmc-release"><day>24</day><month>6</month><year>2009</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>37</volume><issue>15</issue><fpage>5114</fpage><lpage>5125</lpage><history><date date-type="received"><day>28</day><month>1</month><year>2009</year></date><date date-type="rev-recd"><day>30</day><month>4</month><year>2009</year></date><date date-type="accepted"><day>3</day><month>6</month><year>2009</year></date></history><permissions><copyright-statement>© 2009 The Author(s)</copyright-statement><copyright-year>2009</copyright-year><license license-type="creative-commons" xlink:href="http://creativecommons.org/licenses/by-nc/2.0/uk/"><license-p><pmc-comment>CREATIVE COMMONS</pmc-comment>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/2.0/uk/">http://creativecommons.org/licenses/by-nc/2.0/uk/</ext-link>) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><p><italic>Escherichia coli</italic> possesses only one essential oligoribonuclease (Orn), an enzyme that can degrade oligoribonucleotides of five residues and shorter in length (nanoRNA). Firmicutes including <italic>Bacillus subtilis</italic> do not have an Orn homolog. We had previously identified YtqI (NrnA) as functional analog of Orn in <italic>B. subtilis</italic>. Screening a genomic library from <italic>B. subtilis</italic> for genes that can complement a conditional <italic>orn</italic> mutant, we identify here YngD (NrnB) as a second nanoRNase in <italic>B. subtilis</italic>. Like NrnA, NrnB is a member of the DHH/DHHA1 protein family of phosphoesterases. NrnB degrades nanoRNA 5-mers <italic>in vitro</italic> similarily to Orn. Low expression levels of NrnB are sufficient for <italic>orn</italic> complementation. YhaM, a known RNase present in <italic>B. subtilis</italic>, degrades nanoRNA efficiently <italic>in vitro</italic> but requires high levels of expression for only partial complementation of the <italic>orn<sup>–</sup></italic> strain. A triple mutant (<italic>nrnA</italic><sup>–</sup>, <italic>nrnB</italic><sup>–</sup>, <italic>yhaM</italic><sup>–</sup>) in <italic>B. subtilis</italic> is viable and shows almost no impairment in growth. Lastly, RNase J1 seems also to have some 5′-to-3′ exoribonuclease activity on nanoRNA and thus can potentially finish degradation of RNA. We conclude that, unlike in <italic>E. coli</italic>, degradation of nanoRNA is performed in a redundant fashion in <italic>B. subtilis</italic>.</p></abstract></article-meta></front></pmc><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li><li>Villejuif</li></settlement></list><tree><noCountry><name sortKey="Danchin, Antoine" sort="Danchin, Antoine" uniqKey="Danchin A" first="Antoine" last="Danchin">Antoine Danchin</name><name sortKey="Fang, Ming" sort="Fang, Ming" uniqKey="Fang M" first="Ming" last="Fang">Ming Fang</name><name sortKey="Mechold, Undine" sort="Mechold, Undine" uniqKey="Mechold U" first="Undine" last="Mechold">Undine Mechold</name><name sortKey="Zeisberg, Wencke Maria" sort="Zeisberg, Wencke Maria" uniqKey="Zeisberg W" first="Wencke-Maria" last="Zeisberg">Wencke-Maria Zeisberg</name></noCountry><country name="France"><region name="Île-de-France"><name sortKey="Condon, Ciaran" sort="Condon, Ciaran" uniqKey="Condon C" first="Ciaran" last="Condon">Ciaran Condon</name></region><name sortKey="Ogryzko, Vasily" sort="Ogryzko, Vasily" uniqKey="Ogryzko V" first="Vasily" last="Ogryzko">Vasily Ogryzko</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001379 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 001379 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:2731908
|texte= Degradation of nanoRNA is performed by multiple redundant RNases in Bacillus subtilis
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:19553197" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |