Heat inactivation of the Middle East respiratory syndrome coronavirus
Identifieur interne : 001071 ( Pmc/Checkpoint ); précédent : 001070; suivant : 001072Heat inactivation of the Middle East respiratory syndrome coronavirus
Auteurs : India Leclercq [France] ; Christophe Batéjat [France] ; Ana M. Burguière [France] ; Jean-Claude Manuguerra [France]Source :
- Influenza and Other Respiratory Viruses [ 1750-2640 ] ; 2014.
Abstract
The culture supernatants of the emerging Middle East respiratory syndrome coronavirus (MERS-CoV) were submitted to three temperatures over time and tested for infectivity by TCID50 method on Vero E6 cells. At 56°C, almost 25 minutes were necessary to reduce the initial titre by 4 log10. Increasing temperature to 65°C had a strong negative effect on viral infectivity as virucidy dropped significantly to 1 minute. On the contrary, no significant decrease in titre was observed after 2 hours at 25°C. These data might be useful in establishing biosafety measures in laboratories against MERS-CoV.
Url:
DOI: 10.1111/irv.12261
PubMed: 25074677
PubMed Central: 4181824
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Burguière</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks Unit, Institut Pasteur</institution><addr-line>Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Paris</wicri:regionArea></affiliation></author><author><name sortKey="Manuguerra, Jean Claude" sort="Manuguerra, Jean Claude" uniqKey="Manuguerra J" first="Jean-Claude" last="Manuguerra">Jean-Claude Manuguerra</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks Unit, Institut Pasteur</institution><addr-line>Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Paris</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25074677</idno><idno type="pmc">4181824</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181824</idno><idno type="RBID">PMC:4181824</idno><idno type="doi">10.1111/irv.12261</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000D30</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000D30</idno><idno type="wicri:Area/Pmc/Curation">000D30</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000D30</idno><idno type="wicri:Area/Pmc/Checkpoint">001071</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001071</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Heat inactivation of the Middle East respiratory syndrome coronavirus</title><author><name sortKey="Leclercq, India" sort="Leclercq, India" uniqKey="Leclercq I" first="India" last="Leclercq">India Leclercq</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks Unit, Institut Pasteur</institution><addr-line>Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Paris</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Université Paris Diderot, Sorbonne Paris Cité (Cellule Pasteur)</institution><addr-line>Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Paris</wicri:regionArea></affiliation></author><author><name sortKey="Batejat, Christophe" sort="Batejat, Christophe" uniqKey="Batejat C" first="Christophe" last="Batéjat">Christophe Batéjat</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks Unit, Institut Pasteur</institution><addr-line>Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Paris</wicri:regionArea></affiliation></author><author><name sortKey="Burguiere, Ana M" sort="Burguiere, Ana M" uniqKey="Burguiere A" first="Ana M" last="Burguière">Ana M. Burguière</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks Unit, Institut Pasteur</institution><addr-line>Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Paris</wicri:regionArea></affiliation></author><author><name sortKey="Manuguerra, Jean Claude" sort="Manuguerra, Jean Claude" uniqKey="Manuguerra J" first="Jean-Claude" last="Manuguerra">Jean-Claude Manuguerra</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks Unit, Institut Pasteur</institution><addr-line>Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Paris</wicri:regionArea></affiliation></author></analytic><series><title level="j">Influenza and Other Respiratory Viruses</title><idno type="ISSN">1750-2640</idno><idno type="eISSN">1750-2659</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The culture supernatants of the emerging Middle East respiratory syndrome coronavirus (MERS-CoV) were submitted to three temperatures over time and tested for infectivity by TCID<sub>50</sub> method on Vero E6 cells. At 56°C, almost 25 minutes were necessary to reduce the initial titre by 4 log<sub>10</sub>. Increasing temperature to 65°C had a strong negative effect on viral infectivity as virucidy dropped significantly to 1 minute. On the contrary, no significant decrease in titre was observed after 2 hours at 25°C. These data might be useful in establishing biosafety measures in laboratories against MERS-CoV.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Memish, Za" uniqKey="Memish Z">ZA Memish</name></author><author><name sortKey="Mishra, N" uniqKey="Mishra N">N Mishra</name></author><author><name sortKey="Olival, Kj" uniqKey="Olival K">KJ Olival</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reusken, Cb" uniqKey="Reusken C">CB Reusken</name></author><author><name sortKey="Haagmans, Bl" uniqKey="Haagmans B">BL Haagmans</name></author><author><name sortKey="Muller, Ma" uniqKey="Muller M">MA Müller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dublineau, A" uniqKey="Dublineau A">A Dublineau</name></author><author><name sortKey="Batejat, C" uniqKey="Batejat C">C Batéjat</name></author><author><name sortKey="Pinon, A" uniqKey="Pinon A">A Pinon</name></author><author><name sortKey="Burguiere, Am" uniqKey="Burguiere A">AM Burguière</name></author><author><name sortKey="Leclercq, I" uniqKey="Leclercq I">I Leclercq</name></author><author><name sortKey="Manuguerra, J C" uniqKey="Manuguerra J">J-C Manuguerra</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guery, B" uniqKey="Guery B">B Guery</name></author><author><name sortKey="Poissy, J" uniqKey="Poissy J">J Poissy</name></author><author><name sortKey="El Mansouf, L" uniqKey="El Mansouf L">L el Mansouf</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Influenza Other Respir Viruses</journal-id><journal-id journal-id-type="iso-abbrev">Influenza Other Respir Viruses</journal-id><journal-id journal-id-type="publisher-id">irv</journal-id><journal-title-group><journal-title>Influenza and Other Respiratory Viruses</journal-title></journal-title-group><issn pub-type="ppub">1750-2640</issn><issn pub-type="epub">1750-2659</issn><publisher><publisher-name>Blackwell Publishing Ltd</publisher-name><publisher-loc>Oxford, UK</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25074677</article-id><article-id pub-id-type="pmc">4181824</article-id><article-id pub-id-type="doi">10.1111/irv.12261</article-id><article-categories><subj-group subj-group-type="heading"><subject>Short Articles</subject></subj-group></article-categories><title-group><article-title>Heat inactivation of the Middle East respiratory syndrome coronavirus</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Leclercq</surname><given-names>India</given-names></name><xref ref-type="aff" rid="au1">a</xref><xref ref-type="aff" rid="au2">b</xref></contrib><contrib contrib-type="author"><name><surname>Batéjat</surname><given-names>Christophe</given-names></name><xref ref-type="aff" rid="au1">a</xref></contrib><contrib contrib-type="author"><name><surname>Burguière</surname><given-names>Ana M</given-names></name><xref ref-type="aff" rid="au1">a</xref></contrib><contrib contrib-type="author"><name><surname>Manuguerra</surname><given-names>Jean-Claude</given-names></name><xref ref-type="aff" rid="au1">a</xref></contrib><aff id="au1"><label>a</label><institution>Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks Unit, Institut Pasteur</institution><addr-line>Paris, France</addr-line></aff><aff id="au2"><label>b</label><institution>Université Paris Diderot, Sorbonne Paris Cité (Cellule Pasteur)</institution><addr-line>Paris, France</addr-line></aff></contrib-group><author-notes><corresp id="cor1">India Leclercq, Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks Unit, Institut Pasteur, 28 rue du docteur Roux, 75724 Paris, Cedex 15, France. E-mail: <email>india.leclercq@pasteur.fr</email></corresp></author-notes><pub-date pub-type="ppub"><month>9</month><year>2014</year></pub-date><pub-date pub-type="epub"><day>24</day><month>6</month><year>2014</year></pub-date><volume>8</volume><issue>5</issue><fpage>585</fpage><lpage>586</lpage><history><date date-type="accepted"><day>08</day><month>5</month><year>2014</year></date></history><permissions><copyright-statement>© 2014 The Authors. <italic>Influenza and Other Respiratory Viruses</italic> Published by John Wiley & Sons Ltd.</copyright-statement><copyright-year>2014</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/3.0/"><license-p>This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><p>The culture supernatants of the emerging Middle East respiratory syndrome coronavirus (MERS-CoV) were submitted to three temperatures over time and tested for infectivity by TCID<sub>50</sub> method on Vero E6 cells. At 56°C, almost 25 minutes were necessary to reduce the initial titre by 4 log<sub>10</sub>. Increasing temperature to 65°C had a strong negative effect on viral infectivity as virucidy dropped significantly to 1 minute. On the contrary, no significant decrease in titre was observed after 2 hours at 25°C. These data might be useful in establishing biosafety measures in laboratories against MERS-CoV.</p></abstract><kwd-group><kwd>Heat</kwd><kwd>inactivation</kwd><kwd>MERS-CoV</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>France</li></country></list><tree><country name="France"><noRegion><name sortKey="Leclercq, India" sort="Leclercq, India" uniqKey="Leclercq I" first="India" last="Leclercq">India Leclercq</name></noRegion><name sortKey="Batejat, Christophe" sort="Batejat, Christophe" uniqKey="Batejat C" first="Christophe" last="Batéjat">Christophe Batéjat</name><name sortKey="Burguiere, Ana M" sort="Burguiere, Ana M" uniqKey="Burguiere A" first="Ana M" last="Burguière">Ana M. Burguière</name><name sortKey="Leclercq, India" sort="Leclercq, India" uniqKey="Leclercq I" first="India" last="Leclercq">India Leclercq</name><name sortKey="Manuguerra, Jean Claude" sort="Manuguerra, Jean Claude" uniqKey="Manuguerra J" first="Jean-Claude" last="Manuguerra">Jean-Claude Manuguerra</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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