MERS-CoV diagnosis: An update
Identifieur interne : 000B08 ( Pmc/Checkpoint ); précédent : 000B07; suivant : 000B09MERS-CoV diagnosis: An update
Auteurs : Sameera Al Johani ; Ali H. HajeerSource :
- Journal of Infection and Public Health [ 1876-0341 ] ; 2016.
Abstract
Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient's testings.
Url:
DOI: 10.1016/j.jiph.2016.04.005
PubMed: 27106390
PubMed Central: 7102781
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:7102781Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">MERS-CoV diagnosis: An update</title>
<author><name sortKey="Al Johani, Sameera" sort="Al Johani, Sameera" uniqKey="Al Johani S" first="Sameera" last="Al Johani">Sameera Al Johani</name>
</author>
<author><name sortKey="Hajeer, Ali H" sort="Hajeer, Ali H" uniqKey="Hajeer A" first="Ali H." last="Hajeer">Ali H. Hajeer</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">27106390</idno>
<idno type="pmc">7102781</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102781</idno>
<idno type="RBID">PMC:7102781</idno>
<idno type="doi">10.1016/j.jiph.2016.04.005</idno>
<date when="2016">2016</date>
<idno type="wicri:Area/Pmc/Corpus">000E39</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000E39</idno>
<idno type="wicri:Area/Pmc/Curation">000E39</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000E39</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000B08</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000B08</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">MERS-CoV diagnosis: An update</title>
<author><name sortKey="Al Johani, Sameera" sort="Al Johani, Sameera" uniqKey="Al Johani S" first="Sameera" last="Al Johani">Sameera Al Johani</name>
</author>
<author><name sortKey="Hajeer, Ali H" sort="Hajeer, Ali H" uniqKey="Hajeer A" first="Ali H." last="Hajeer">Ali H. Hajeer</name>
</author>
</analytic>
<series><title level="j">Journal of Infection and Public Health</title>
<idno type="ISSN">1876-0341</idno>
<idno type="eISSN">1876-035X</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>Summary</title>
<p>Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient's testings.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name>
</author>
<author><name sortKey="Al Tawfiq, J A" uniqKey="Al Tawfiq J">J.A. Al-Tawfiq</name>
</author>
<author><name sortKey="Makhdoom, H Q" uniqKey="Makhdoom H">H.Q. Makhdoom</name>
</author>
<author><name sortKey="Assiri, A" uniqKey="Assiri A">A. Assiri</name>
</author>
<author><name sortKey="Alhakeem, R F" uniqKey="Alhakeem R">R.F. Alhakeem</name>
</author>
<author><name sortKey="Albarrak, A" uniqKey="Albarrak A">A. Albarrak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Guery, B" uniqKey="Guery B">B. Guery</name>
</author>
<author><name sortKey="Poissy, J" uniqKey="Poissy J">J. Poissy</name>
</author>
<author><name sortKey="El Mansouf, L" uniqKey="El Mansouf L">L. el Mansouf</name>
</author>
<author><name sortKey="Sejourne, C" uniqKey="Sejourne C">C. Sejourne</name>
</author>
<author><name sortKey="Ettahar, N" uniqKey="Ettahar N">N. Ettahar</name>
</author>
<author><name sortKey="Lemaire, X" uniqKey="Lemaire X">X. Lemaire</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ulmer, A J" uniqKey="Ulmer A">A.J. Ulmer</name>
</author>
<author><name sortKey="Mattern, T" uniqKey="Mattern T">T. Mattern</name>
</author>
<author><name sortKey="Flad, H D" uniqKey="Flad H">H.D. Flad</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Feikin, D R" uniqKey="Feikin D">D.R. Feikin</name>
</author>
<author><name sortKey="Alraddadi, B" uniqKey="Alraddadi B">B. Alraddadi</name>
</author>
<author><name sortKey="Qutub, M" uniqKey="Qutub M">M. Qutub</name>
</author>
<author><name sortKey="Shabouni, O" uniqKey="Shabouni O">O. Shabouni</name>
</author>
<author><name sortKey="Curns, A" uniqKey="Curns A">A. Curns</name>
</author>
<author><name sortKey="Oboho, I K" uniqKey="Oboho I">I.K. Oboho</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name>
</author>
<author><name sortKey="Olschlager, S" uniqKey="Olschlager S">S. Olschlager</name>
</author>
<author><name sortKey="Wendtner, C M" uniqKey="Wendtner C">C.M. Wendtner</name>
</author>
<author><name sortKey="Drexler, J F" uniqKey="Drexler J">J.F. Drexler</name>
</author>
<author><name sortKey="Hess, M" uniqKey="Hess M">M. Hess</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Seong, M W" uniqKey="Seong M">M.W. Seong</name>
</author>
<author><name sortKey="Lee, S J" uniqKey="Lee S">S.J. Lee</name>
</author>
<author><name sortKey="Cho, S I" uniqKey="Cho S">S.I. Cho</name>
</author>
<author><name sortKey="Ko, K" uniqKey="Ko K">K. Ko</name>
</author>
<author><name sortKey="Kim, M N" uniqKey="Kim M">M.N. Kim</name>
</author>
<author><name sortKey="Sung, H" uniqKey="Sung H">H. Sung</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pas, S D" uniqKey="Pas S">S.D. Pas</name>
</author>
<author><name sortKey="Patel, P" uniqKey="Patel P">P. Patel</name>
</author>
<author><name sortKey="Reusken, C" uniqKey="Reusken C">C. Reusken</name>
</author>
<author><name sortKey="Domingo, C" uniqKey="Domingo C">C. Domingo</name>
</author>
<author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. Zaki</name>
</author>
<author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name>
</author>
<author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name>
</author>
<author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name>
</author>
<author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Seong, M W" uniqKey="Seong M">M.W. Seong</name>
</author>
<author><name sortKey="Kim, S Y" uniqKey="Kim S">S.Y. Kim</name>
</author>
<author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name>
</author>
<author><name sortKey="Kim, T S" uniqKey="Kim T">T.S. Kim</name>
</author>
<author><name sortKey="Cho, S I" uniqKey="Cho S">S.I. Cho</name>
</author>
<author><name sortKey="Kim, M J" uniqKey="Kim M">M.J. Kim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Borucki, M K" uniqKey="Borucki M">M.K. Borucki</name>
</author>
<author><name sortKey="Lao, V" uniqKey="Lao V">V. Lao</name>
</author>
<author><name sortKey="Hwang, M" uniqKey="Hwang M">M. Hwang</name>
</author>
<author><name sortKey="Gardner, S" uniqKey="Gardner S">S. Gardner</name>
</author>
<author><name sortKey="Adney, D" uniqKey="Adney D">D. Adney</name>
</author>
<author><name sortKey="Munster, V" uniqKey="Munster V">V. Munster</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Assiri, A" uniqKey="Assiri A">A. Assiri</name>
</author>
<author><name sortKey="Abedi, G R" uniqKey="Abedi G">G.R. Abedi</name>
</author>
<author><name sortKey="Saeed, A A" uniqKey="Saeed A">A.A. Saeed</name>
</author>
<author><name sortKey="Abdalla, M A" uniqKey="Abdalla M">M.A. Abdalla</name>
</author>
<author><name sortKey="Al Masry, M" uniqKey="Al Masry M">M. Al-Masry</name>
</author>
<author><name sortKey="Choudhry, A J" uniqKey="Choudhry A">A.J. Choudhry</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kim, Y" uniqKey="Kim Y">Y. Kim</name>
</author>
<author><name sortKey="Cheon, S" uniqKey="Cheon S">S. Cheon</name>
</author>
<author><name sortKey="Min, C K" uniqKey="Min C">C.K. Min</name>
</author>
<author><name sortKey="Sohn, K M" uniqKey="Sohn K">K.M. Sohn</name>
</author>
<author><name sortKey="Kang, Y J" uniqKey="Kang Y">Y.J. Kang</name>
</author>
<author><name sortKey="Cha, Y J" uniqKey="Cha Y">Y.J. Cha</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Park, W B" uniqKey="Park W">W.B. Park</name>
</author>
<author><name sortKey="Perera, R A" uniqKey="Perera R">R.A. Perera</name>
</author>
<author><name sortKey="Choe, P G" uniqKey="Choe P">P.G. Choe</name>
</author>
<author><name sortKey="Lau, E H" uniqKey="Lau E">E.H. Lau</name>
</author>
<author><name sortKey="Choi, S J" uniqKey="Choi S">S.J. Choi</name>
</author>
<author><name sortKey="Chun, J Y" uniqKey="Chun J">J.Y. Chun</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y. Chen</name>
</author>
<author><name sortKey="Chan, K H" uniqKey="Chan K">K.H. Chan</name>
</author>
<author><name sortKey="Kang, Y" uniqKey="Kang Y">Y. Kang</name>
</author>
<author><name sortKey="Chen, H" uniqKey="Chen H">H. Chen</name>
</author>
<author><name sortKey="Luk, H" uniqKey="Luk H">H. Luk</name>
</author>
<author><name sortKey="Poon, R W" uniqKey="Poon R">R.W. Poon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name>
</author>
<author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Perera, R A" uniqKey="Perera R">R.A. Perera</name>
</author>
<author><name sortKey="Wang, P" uniqKey="Wang P">P. Wang</name>
</author>
<author><name sortKey="Gomaa, M R" uniqKey="Gomaa M">M.R. Gomaa</name>
</author>
<author><name sortKey="El Shesheny, R" uniqKey="El Shesheny R">R. El-Shesheny</name>
</author>
<author><name sortKey="Kandeil, A" uniqKey="Kandeil A">A. Kandeil</name>
</author>
<author><name sortKey="Bagato, O" uniqKey="Bagato O">O. Bagato</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Buchholz, U" uniqKey="Buchholz U">U. Buchholz</name>
</author>
<author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name>
</author>
<author><name sortKey="Nitsche, A" uniqKey="Nitsche A">A. Nitsche</name>
</author>
<author><name sortKey="Sanewski, A" uniqKey="Sanewski A">A. Sanewski</name>
</author>
<author><name sortKey="Wevering, N" uniqKey="Wevering N">N. Wevering</name>
</author>
<author><name sortKey="Bauer Balci, T" uniqKey="Bauer Balci T">T. Bauer-Balci</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name>
</author>
<author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name>
</author>
<author><name sortKey="Costabel, U" uniqKey="Costabel U">U. Costabel</name>
</author>
<author><name sortKey="Timm, J" uniqKey="Timm J">J. Timm</name>
</author>
<author><name sortKey="Binger, T" uniqKey="Binger T">T. Binger</name>
</author>
<author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name>
</author>
<author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name>
</author>
<author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name>
</author>
<author><name sortKey="Al Masri, M" uniqKey="Al Masri M">M. Al-Masri</name>
</author>
<author><name sortKey="Turkestani, A" uniqKey="Turkestani A">A. Turkestani</name>
</author>
<author><name sortKey="Ritz, D" uniqKey="Ritz D">D. Ritz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, Y D" uniqKey="Wang Y">Y.D. Wang</name>
</author>
<author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name>
</author>
<author><name sortKey="Xu, G B" uniqKey="Xu G">G.B. Xu</name>
</author>
<author><name sortKey="Dong, X Y" uniqKey="Dong X">X.Y. Dong</name>
</author>
<author><name sortKey="Yang, X A" uniqKey="Yang X">X.A. Yang</name>
</author>
<author><name sortKey="Feng, Z R" uniqKey="Feng Z">Z.R. Feng</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Infect Public Health</journal-id>
<journal-id journal-id-type="iso-abbrev">J Infect Public Health</journal-id>
<journal-title-group><journal-title>Journal of Infection and Public Health</journal-title>
</journal-title-group>
<issn pub-type="ppub">1876-0341</issn>
<issn pub-type="epub">1876-035X</issn>
<publisher><publisher-name>Elsevier</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">27106390</article-id>
<article-id pub-id-type="pmc">7102781</article-id>
<article-id pub-id-type="publisher-id">S1876-0341(16)30022-3</article-id>
<article-id pub-id-type="doi">10.1016/j.jiph.2016.04.005</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>MERS-CoV diagnosis: An update</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" id="aut0005"><name><surname>Al Johani</surname>
<given-names>Sameera</given-names>
</name>
<email>johanis@ngha.med.sa</email>
<xref rid="cor0005" ref-type="corresp">⁎</xref>
</contrib>
<contrib contrib-type="author" id="aut0010"><name><surname>Hajeer</surname>
<given-names>Ali H.</given-names>
</name>
</contrib>
</contrib-group>
<aff id="aff0005">Department of Pathology and Laboratory Medicine, King Saud University for Health Sciences, Riyadh, Saudi Arabia</aff>
<author-notes><corresp id="cor0005"><label>⁎</label>
Corresponding author at: Division of Microbiology, Pathology and Laboratory Medicine, Mail Code 1122, King Abdulaziz Medical City, P.O. Box 22490, Riyadh 11426, Saudi Arabia. Tel.: +966 558891414. <email>johanis@ngha.med.sa</email>
</corresp>
</author-notes>
<pub-date pub-type="pmc-release"><day>20</day>
<month>4</month>
<year>2016</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><season>May-Jun</season>
<year>2016</year>
</pub-date>
<pub-date pub-type="epub"><day>20</day>
<month>4</month>
<year>2016</year>
</pub-date>
<volume>9</volume>
<issue>3</issue>
<fpage>216</fpage>
<lpage>219</lpage>
<history><date date-type="received"><day>4</day>
<month>4</month>
<year>2016</year>
</date>
<date date-type="accepted"><day>5</day>
<month>4</month>
<year>2016</year>
</date>
</history>
<permissions><copyright-statement>© 2016 King Saud Bin Abdulaziz University for Health Sciences. Production and hosting by Elsevier Limited.</copyright-statement>
<copyright-year>2016</copyright-year>
<copyright-holder>King Saud Bin Abdulaziz University for Health Sciences</copyright-holder>
<license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p>
</license>
</permissions>
<abstract id="abs1005"><title>Summary</title>
<p>Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient's testings.</p>
</abstract>
<kwd-group id="kwd0005"><title>Keywords</title>
<kwd>MERS-COV</kwd>
<kwd>RT-PCR</kwd>
<kwd>Serology</kwd>
<kwd>Sequencing</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Al Johani, Sameera" sort="Al Johani, Sameera" uniqKey="Al Johani S" first="Sameera" last="Al Johani">Sameera Al Johani</name>
<name sortKey="Hajeer, Ali H" sort="Hajeer, Ali H" uniqKey="Hajeer A" first="Ali H." last="Hajeer">Ali H. Hajeer</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B08 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000B08 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Pmc |étape= Checkpoint |type= RBID |clé= PMC:7102781 |texte= MERS-CoV diagnosis: An update }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:27106390" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |