Structural characterization of the N‐terminal part of the MERS‐CoV nucleocapsid by X‐ray diffraction and small‐angle X‐ray scattering
Identifieur interne : 000A00 ( Pmc/Checkpoint ); précédent : 000999; suivant : 000A01Structural characterization of the N‐terminal part of the MERS‐CoV nucleocapsid by X‐ray diffraction and small‐angle X‐ray scattering
Auteurs : Nicolas Papageorgiou ; Julie Lichière ; Amal Baklouti ; François Ferron ; Marion Sévajol ; Bruno Canard ; Bruno CoutardSource :
- Acta Crystallographica. Section D, Structural Biology [ 2059-7983 ] ; 2016.
Abstract
The N protein of coronaviruses is a multifunctional protein that is organized into several domains. The N‐terminal part is composed of an intrinsically disordered region (IDR) followed by a structured domain called the N‐terminal domain (NTD). In this study, the structure determination of the N‐terminal region of the MERS‐CoV N protein
Url:
DOI: 10.1107/S2059798315024328
PubMed: 26894667
PubMed Central: 7159594
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:7159594Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structural characterization of the N‐terminal part of the MERS‐CoV nucleocapsid by X‐ray diffraction and small‐angle X‐ray scattering</title><author><name sortKey="Papageorgiou, Nicolas" sort="Papageorgiou, Nicolas" uniqKey="Papageorgiou N" first="Nicolas" last="Papageorgiou">Nicolas Papageorgiou</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author><author><name sortKey="Lichiere, Julie" sort="Lichiere, Julie" uniqKey="Lichiere J" first="Julie" last="Lichière">Julie Lichière</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author><author><name sortKey="Baklouti, Amal" sort="Baklouti, Amal" uniqKey="Baklouti A" first="Amal" last="Baklouti">Amal Baklouti</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author><author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author><author><name sortKey="Sevajol, Marion" sort="Sevajol, Marion" uniqKey="Sevajol M" first="Marion" last="Sévajol">Marion Sévajol</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author><author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author><author><name sortKey="Coutard, Bruno" sort="Coutard, Bruno" uniqKey="Coutard B" first="Bruno" last="Coutard">Bruno Coutard</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26894667</idno><idno type="pmc">7159594</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7159594</idno><idno type="RBID">PMC:7159594</idno><idno type="doi">10.1107/S2059798315024328</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000977</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000977</idno><idno type="wicri:Area/Pmc/Curation">000977</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000977</idno><idno type="wicri:Area/Pmc/Checkpoint">000A00</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000A00</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Structural characterization of the N‐terminal part of the MERS‐CoV nucleocapsid by X‐ray diffraction and small‐angle X‐ray scattering</title><author><name sortKey="Papageorgiou, Nicolas" sort="Papageorgiou, Nicolas" uniqKey="Papageorgiou N" first="Nicolas" last="Papageorgiou">Nicolas Papageorgiou</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author><author><name sortKey="Lichiere, Julie" sort="Lichiere, Julie" uniqKey="Lichiere J" first="Julie" last="Lichière">Julie Lichière</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author><author><name sortKey="Baklouti, Amal" sort="Baklouti, Amal" uniqKey="Baklouti A" first="Amal" last="Baklouti">Amal Baklouti</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author><author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author><author><name sortKey="Sevajol, Marion" sort="Sevajol, Marion" uniqKey="Sevajol M" first="Marion" last="Sévajol">Marion Sévajol</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author><author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author><author><name sortKey="Coutard, Bruno" sort="Coutard, Bruno" uniqKey="Coutard B" first="Bruno" last="Coutard">Bruno Coutard</name><affiliation><nlm:aff id="AYD2MN5105-aff-a1"></nlm:aff></affiliation><affiliation><nlm:aff id="AYD2MN5105-aff-a2"></nlm:aff></affiliation></author></analytic><series><title level="j">Acta Crystallographica. Section D, Structural Biology</title><idno type="eISSN">2059-7983</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The N protein of coronaviruses is a multifunctional protein that is organized into several domains. The N‐terminal part is composed of an intrinsically disordered region (IDR) followed by a structured domain called the N‐terminal domain (NTD). In this study, the structure determination of the N‐terminal region of the MERS‐CoV N protein <italic>via</italic> X‐ray diffraction measurements is reported at a resolution of 2.4 Å. Since the first 30 amino acids were not resolved by X‐ray diffraction, the structural study was completed by a SAXS experiment to propose a structural model including the IDR. This model presents the N‐terminal region of the MERS‐CoV as a monomer that displays structural features in common with other coronavirus NTDs.</p></div></front></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Acta Crystallogr D Struct Biol</journal-id><journal-id journal-id-type="iso-abbrev">Acta Crystallogr D Struct Biol</journal-id><journal-id journal-id-type="doi">10.1107/S20597983</journal-id><journal-id journal-id-type="publisher-id">AYD2</journal-id><journal-title-group><journal-title>Acta Crystallographica. Section D, Structural Biology</journal-title></journal-title-group><issn pub-type="epub">2059-7983</issn><publisher><publisher-name>International Union of Crystallography</publisher-name><publisher-loc>5 Abbey Square, Chester, Cheshire CH1 2HU, England</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26894667</article-id><article-id pub-id-type="pmc">7159594</article-id><article-id pub-id-type="doi">10.1107/S2059798315024328</article-id><article-id pub-id-type="publisher-id">AYD2MN5105</article-id><article-id pub-id-type="other">MN5105</article-id><article-categories><subj-group subj-group-type="overline"><subject>Research Papers</subject></subj-group><subj-group subj-group-type="heading"><subject>Research Papers</subject></subj-group></article-categories><title-group><article-title>Structural characterization of the N‐terminal part of the MERS‐CoV nucleocapsid by X‐ray diffraction and small‐angle X‐ray scattering</article-title><alt-title alt-title-type="right-running-head">N‐terminal part of the MERS‐CoV nucleocapsid</alt-title></title-group><contrib-group><contrib id="AYD2MN5105-cr-1" contrib-type="author" corresp="yes"><name><surname>Papageorgiou</surname><given-names>Nicolas</given-names></name><xref ref-type="aff" rid="AYD2MN5105-aff-a1"><sup>1</sup></xref><xref ref-type="aff" rid="AYD2MN5105-aff-a2"><sup>2</sup></xref></contrib><contrib id="AYD2MN5105-cr-2" contrib-type="author"><name><surname>Lichière</surname><given-names>Julie</given-names></name><xref ref-type="aff" rid="AYD2MN5105-aff-a1"><sup>1</sup></xref><xref ref-type="aff" rid="AYD2MN5105-aff-a2"><sup>2</sup></xref></contrib><contrib id="AYD2MN5105-cr-3" contrib-type="author"><name><surname>Baklouti</surname><given-names>Amal</given-names></name><xref ref-type="aff" rid="AYD2MN5105-aff-a1"><sup>1</sup></xref><xref ref-type="aff" rid="AYD2MN5105-aff-a2"><sup>2</sup></xref></contrib><contrib id="AYD2MN5105-cr-4" contrib-type="author"><name><surname>Ferron</surname><given-names>François</given-names></name><xref ref-type="aff" rid="AYD2MN5105-aff-a1"><sup>1</sup></xref><xref ref-type="aff" rid="AYD2MN5105-aff-a2"><sup>2</sup></xref></contrib><contrib id="AYD2MN5105-cr-5" contrib-type="author"><name><surname>Sévajol</surname><given-names>Marion</given-names></name><xref ref-type="aff" rid="AYD2MN5105-aff-a1"><sup>1</sup></xref><xref ref-type="aff" rid="AYD2MN5105-aff-a2"><sup>2</sup></xref></contrib><contrib id="AYD2MN5105-cr-6" contrib-type="author"><name><surname>Canard</surname><given-names>Bruno</given-names></name><xref ref-type="aff" rid="AYD2MN5105-aff-a1"><sup>1</sup></xref><xref ref-type="aff" rid="AYD2MN5105-aff-a2"><sup>2</sup></xref></contrib><contrib id="AYD2MN5105-cr-7" contrib-type="author" corresp="yes"><name><surname>Coutard</surname><given-names>Bruno</given-names></name><xref ref-type="aff" rid="AYD2MN5105-aff-a1"><sup>1</sup></xref><xref ref-type="aff" rid="AYD2MN5105-aff-a2"><sup>2</sup></xref></contrib></contrib-group><aff id="AYD2MN5105-aff-a1"><label><sup>1</sup></label>CNRS, AFMB UMR 7257, 13288Marseille, France</aff><aff id="AYD2MN5105-aff-a2"><label><sup>2</sup></label>Aix-Marseille Université, AFMB UMR 7257, 13288Marseille, France</aff><author-notes><corresp id="correspondenceTo"><label>*</label>Nicolas Papageorgiou, e-mail: <email>nicolas.papageorgiou@afmb.univ-mrs.fr</email>; Bruno Coutard, e-mail: <email>bruno.coutard@afmb.univ-mrs.fr</email></corresp></author-notes><pub-date pub-type="epub"><day>04</day><month>2</month><year>2016</year></pub-date><pub-date pub-type="ppub"><month>2</month><year>2016</year></pub-date><volume>72</volume><issue>2</issue><issue-id pub-id-type="doi">10.1107/S20597983720200</issue-id><fpage>192</fpage><lpage>202</lpage><history><date date-type="received"><day>05</day><month>10</month><year>2015</year></date><date date-type="accepted"><day>17</day><month>12</month><year>2015</year></date></history><permissions><copyright-statement content-type="article-copyright">© International Union of Crystallography, 2016</copyright-statement><license><license-p>This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="file:AYD2-72-192.pdf"></self-uri><abstract><p>The N protein of coronaviruses is a multifunctional protein that is organized into several domains. The N‐terminal part is composed of an intrinsically disordered region (IDR) followed by a structured domain called the N‐terminal domain (NTD). In this study, the structure determination of the N‐terminal region of the MERS‐CoV N protein <italic>via</italic> X‐ray diffraction measurements is reported at a resolution of 2.4 Å. Since the first 30 amino acids were not resolved by X‐ray diffraction, the structural study was completed by a SAXS experiment to propose a structural model including the IDR. This model presents the N‐terminal region of the MERS‐CoV as a monomer that displays structural features in common with other coronavirus NTDs.</p></abstract><abstract abstract-type="graphical"><p>The structural characterization of the N‐terminal part of the nucleocapsid from <italic>Middle East respiratory syndrome coronavirus</italic> (MERS‐CoV), a recently emerging virus, is reported. The structure of the N‐terminal region, which includes a disordered tail followed by a globular domain, was obtained by combining X‐ray diffraction and SAXS.<boxed-text position="anchor" content-type="graphic" orientation="portrait"><graphic xlink:href="AYD2-72-192-g001.jpg" position="anchor" id="nlm-graphic-1" orientation="portrait"></graphic></boxed-text></p></abstract><kwd-group><kwd id="AYD2MN5105-kwd-1">MERS‐CoV</kwd><kwd id="AYD2MN5105-kwd-2">nucleocapsid</kwd><kwd id="AYD2MN5105-kwd-3">structure</kwd><kwd id="AYD2MN5105-kwd-4">SAXS</kwd><kwd id="AYD2MN5105-kwd-5">RNA‐binding domain</kwd></kwd-group><counts><page-count count="10"></page-count></counts><custom-meta-group><custom-meta><meta-name>source-schema-version-number</meta-name><meta-value>2.0</meta-value></custom-meta><custom-meta><meta-name>cover-date</meta-name><meta-value>February 2016</meta-value></custom-meta><custom-meta><meta-name>details-of-publishers-convertor</meta-name><meta-value>Converter:WILEY_ML3GV2_TO_JATSPMC version:5.8.0 mode:remove_FC converted:15.04.2020</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Baklouti, Amal" sort="Baklouti, Amal" uniqKey="Baklouti A" first="Amal" last="Baklouti">Amal Baklouti</name><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><name sortKey="Coutard, Bruno" sort="Coutard, Bruno" uniqKey="Coutard B" first="Bruno" last="Coutard">Bruno Coutard</name><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name><name sortKey="Lichiere, Julie" sort="Lichiere, Julie" uniqKey="Lichiere J" first="Julie" last="Lichière">Julie Lichière</name><name sortKey="Papageorgiou, Nicolas" sort="Papageorgiou, Nicolas" uniqKey="Papageorgiou N" first="Nicolas" last="Papageorgiou">Nicolas Papageorgiou</name><name sortKey="Sevajol, Marion" sort="Sevajol, Marion" uniqKey="Sevajol M" first="Marion" last="Sévajol">Marion Sévajol</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A00 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000A00 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:7159594
|texte= Structural characterization of the N‐terminal part of the MERS‐CoV nucleocapsid by X‐ray diffraction and small‐angle X‐ray scattering
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:26894667" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |